- Title
- Sequence and domain conservation of the coelacanth Hsp40 and Hsp90 chaperones suggests conservation of function
- Creator
- Tastan Bishop, Özlem, Edkins, Adrienne L, Blatch, Gregory L
- Date
- 2014
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/126932
- Identifier
- vital:35936
- Identifier
- https://doi.10.1002/jez.b.22541
- Description
- Molecular chaperones and their associated co‐chaperones play an important role in preserving and regulating the active conformational state of cellular proteins. The chaperone complement of the Indonesian Coelacanth, Latimeria menadoensis, was elucidated using transcriptomic sequences. Heat shock protein 90 (Hsp90) and heat shock protein 40 (Hsp40) chaperones, and associated cochaperones were focused on, and homologous human sequences were used to search the sequence databases. Coelacanth homologs of the cytosolic, mitochondrial and endoplasmic reticulum (ER) homologs of human Hsp90 were identified, as well as all of the major co‐chaperones of the cytosolic isoform. Most of the human Hsp40s were found to have coelacanth homologs, and the data suggested that all of the chaperone machinery for protein folding at the ribosome, protein translocation to cellular compartments such as the ER and protein degradation were conserved. Some interesting similarities and differences were identified when interrogating human, mouse, and zebrafish homologs. For example, DnaJB13 is predicted to be a non‐functional Hsp40 in humans, mouse, and zebrafish due to a corrupted histidine‐proline‐aspartic acid (HPD) motif, while the coelacanth homolog has an intact HPD. These and other comparisons enabled important functional and evolutionary questions to be posed for future experimental studies.
- Format
- 20 pages, pdf
- Language
- English
- Relation
- Journal of experimental zoology part B: molecular and developmental evolution, Tastan Bishop, Ö., Edkins, A.L. and Blatch, G.L., 2014. Sequence and domain conservation of the coelacanth Hsp40 and Hsp90 chaperones suggests conservation of function. Journal of Experimental Zoology Part B: Molecular and Developmental Evolution, 322(6), pp.359-378, Journal of experimental zoology part B: molecular and developmental evolution volume 322 number 6 359 378 2014 1552-5007
- Rights
- Journal of Experimental zoology part B: molecular and developmental evolution
- Rights
- Use of this resource is governed by the terms and conditions of the Wiley Online Library Terms of Use statement (https://onlinelibrary.wiley.com/terms-and-conditions)
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Edkins, Adrienne Lesley (Prof)
Tastan Bishop, Ozlem (Prof)
Blatch, Gregory Lloyd (Prof)
RU Research Unit in Bioinformatics (RUBi)
SDG 03. Good Health and Well-Being
RU Department of Biochemistry, Microbiology and Bioinformatics
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