The biotransformation of phenolic pollutants using polyphenol oxidase

Title: The biotransformation of phenolic pollutants using polyphenol oxidase
Creator: Boshoff, Aileen
Subject: Polyphenol oxidase Sewage -- Purification
Date: 2002
Type: Thesis
Type: Doctoral
Type: PhD
Identifier: vital:3976
Identifier: http://hdl.handle.net/10962/d1004035
Description: The potential of using mushroom polyphenol oxidase (EC 1.14.18.1) as a biocatalyst for the biotransformation of phenols to produce catechols in an aqueous medium was investigated. Polyphenol oxidase is characterised by two distinct reactions i.e., the ortho-hydroxylation of phenols to catechols (cresolase activity) and the subsequent oxidation of catechols to orthoquinones (catecholase activity). In order to facilitate the development of a process to produce catechols, the accumulation of catechol as a true intermediate product released in the reaction system needed to be investigated, as its release had been disputed due to the oxidation of catechols to o-quinones. Using LC-MS, catechol products were successfully identified as true intermediate products formed during biocatalytic reactions in water.
Format: 227 leaves, pdf
Publisher: Rhodes University, Faculty of Science, Biochemistry, Microbiology and Biotechnology
Language: English
Rights: Boshoff, Aileen

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RU Department of Biochemistry, Microbiology and Biotechnology (end of 2014)

Boshoff, Aileen (Assoc Prof)

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