- Title
- The biotransformation of phenolic pollutants using polyphenol oxidase
- Creator
- Boshoff, Aileen
- Subject
- Polyphenol oxidase Sewage -- Purification
- Date
- 2002
- Type
- Thesis
- Type
- Doctoral
- Type
- PhD
- Identifier
- vital:3976
- Identifier
- http://hdl.handle.net/10962/d1004035
- Description
- The potential of using mushroom polyphenol oxidase (EC 1.14.18.1) as a biocatalyst for the biotransformation of phenols to produce catechols in an aqueous medium was investigated. Polyphenol oxidase is characterised by two distinct reactions i.e., the ortho-hydroxylation of phenols to catechols (cresolase activity) and the subsequent oxidation of catechols to orthoquinones (catecholase activity). In order to facilitate the development of a process to produce catechols, the accumulation of catechol as a true intermediate product released in the reaction system needed to be investigated, as its release had been disputed due to the oxidation of catechols to o-quinones. Using LC-MS, catechol products were successfully identified as true intermediate products formed during biocatalytic reactions in water.
- Format
- 227 leaves, pdf
- Publisher
- Rhodes University, Faculty of Science, Biochemistry, Microbiology and Biotechnology
- Language
- English
- Rights
- Boshoff, Aileen
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RU Department of Biochemistry, Microbiology and Biotechnology (end of 2014)
Boshoff, Aileen (Assoc Prof)
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