- Title
- Real-time monitoring of 3T3-L1 preadipocyte differentiation using a commercially available electric cell-substrate impedance sensor system:
- Creator
- Kramer, Adam H, Joos-Vandewalle, Julia, Edkins, Adrienne L, Frost, Carminita L, Prinsloo, Earl
- Date
- 2014
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/164830
- Identifier
- vital:41176
- Identifier
- DOI: 10.1016/j.bbrc.2013.12.123
- Description
- Real-time analysis offers multiple benefits over traditional end point assays. Here, we present a method of monitoring the optimisation of the growth and differentiation of murine 3T3-L1 preadipocytes to adipocytes using the commercially available ACEA xCELLigence Real-Time Cell Analyser Single Plate (RTCA SP) system. Our findings indicate that the ACEA xCELLigence RTCA SP can reproducibly monitor the primary morphological changes in pre- and post-confluent 3T3-L1 fibroblasts induced to differentiate using insulin, dexamethasone, 3-isobutyl-1-methylxanthine and rosiglitazone; and may be a viable primary method of screening compounds for adipogenic factors.
- Format
- 6 pages, pdf
- Language
- English
- Relation
- Biochemical and biophysical research communications, Kramer, A.H., Joos-Vandewalle, J., Edkins, A.L., Frost, C.L. and Prinsloo, E., 2014. Real-time monitoring of 3T3-L1 preadipocyte differentiation using a commercially available electric cell-substrate impedance sensor system. Biochemical and biophysical research communications, 443(4), pp.1245-1250, Biochemical and biophysical research communications volume 443 number 4 1245 1250 January 2014 1090-2104
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the Elsevier Terms and Conditions Statement (https://www.elsevier.com/legal/elsevier-website-terms-and-conditions)
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Prinsloo, Earl (Dr)
RU DST/NRF SARChI for Molecular and Cellular Biology of the Eukaryotic Stress Response
Edkins, Adrienne Lesley (Associate Prof)
SDG 03. Good Health and Well-Being
RU Department of Biochemistry, Microbiology and Bioinformatics
SDG 09. Industry, Innovation and Infrastructure
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details | SOURCE1 | Real-time monitoring of 3T3-L1 preadipocyte differentiation.pdf | 683 KB | Adobe Acrobat PDF | View Details |