- Title
- Theiler’s murine encephalomyelitis virus infection induces a redistribution of heat shock proteins 70 and 90 in BHK-21 cells, and is inhibited by novobiocin and geldanamycin:
- Creator
- Mutsvunguma, Lorraine Z, Moetlhoa, Boitumelo, Edkins, Adrienne L, Luke, Garry A, Blatch, Gregory L, Knox, Caroline M
- Date
- 2011
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/165085
- Identifier
- vital:41207
- Identifier
- DOI: 10.1007/s12192-011-0262-x
- Description
- Theiler’s murine encephalomyelitis virus (TMEV) is a positive-sense RNA virus belonging to the Cardiovirus genus in the family Picornaviridae. In addition to other host cellular factors and pathways, picornaviruses utilise heat shock proteins (Hsps) to facilitate their propagation in cells. This study investigated the localisation of Hsps 70 and 90 in TMEV-infected BHK-21 cells by indirect immunofluorescence and confocal microscopy. The effect of Hsp90 inhibitors novobiocin (Nov) and geldanamycin (GA) on the development of cytopathic effect (CPE) induced by infection was also examined. Hsp90 staining was uniformly distributed in the cytoplasm of uninfected cells but was found concentrated in the perinuclear region during late infection where it overlapped with the signal for non-structural protein 2C within the viral replication complex.
- Format
- 11 pages, pdf
- Language
- English
- Relation
- Cell Stress and Chaperones, Mutsvunguma, L.Z., Moetlhoa, B., Edkins, A.L., Luke, G.A., Blatch, G.L. and Knox, C., 2011. Theiler’s murine encephalomyelitis virus infection induces a redistribution of heat shock proteins 70 and 90 in BHK-21 cells, and is inhibited by novobiocin and geldanamycin. Cell Stress and Chaperones, 16(5), pp.505-515, Cell Stress and Chaperones volume 16 number 5 505 515 March 2011 1466-1268
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the SpringerLink Terms of Use Statement ( https://link.springer.com/termsandconditions)
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Edkins, Adrienne Lesley (Associate Prof)
Knox, Caroline M (Prof)
Blatch, Gregory Lloyd (Prof)
RU Department of Biochemistry, Microbiology and Bioinformatics
SDG 03. Good Health and Well-Being
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