- Title
- Hsp90α/β associates with the GSK3β/axin1/phospho-β-catenin complex in the human MCF-7 epithelial breast cancer model:
- Creator
- Cooper, Leanne C, Prinsloo, Earl, Edkins, Adrienne L, Blatch, Gregory L
- Date
- 2011
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/165096
- Identifier
- vital:41208
- Identifier
- DOI: 10.1016/j.bbrc.2011.08.136
- Description
- Hsp90α/β, the signal transduction chaperone, maintains intracellular communication in normal, stem, and cancer cells. The well characterised association of Hsp90α/β with its client kinases form the framework of multiple signalling networks. GSK3β, a known Hsp90α/β client, mediates β-catenin phosphorylation as part of a cytoplasmic destruction complex which targets phospho-β-catenin to the 26S proteasome. The canonical Wnt/β-catenin pathway promotes stem cell self-renewal as well as oncogenesis. The degree of Hsp90α/β involvement in Wnt/β-catenin signalling needs clarification. Here, we describe the association of Hsp90α/β with GSK3β, β-catenin, phospho-β-catenin and the molecular scaffold, axin1, in the human MCF-7 epithelial breast cancer cell model using selective inhibition of Hsp90α/β, confocal laser scanning microscopy and immunoprecipitation. Our findings suggest that Hsp90α/β modulates the phosphorylation of β-catenin by interaction in common complex with GSK3β/axin1/β-catenin.
- Format
- 5 pages, pdf
- Language
- English
- Relation
- Biochemical and biophysical research communications, Cooper, L.C., Prinsloo, E., Edkins, A.L. and Blatch, G.L., 2011. Hsp90α/β associates with the GSK3β/axin1/phospho-β-catenin complex in the human MCF-7 epithelial breast cancer model. Biochemical and biophysical research communications, 413(4), pp.550-554, Biochemical and biophysical research communications volume 413 number 4 550 554 October 2011 1090-2104
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the Elsevier Terms and Conditions Statement (https://www.elsevier.com/legal/elsevier-website-terms-and-conditions)
- Hits: 2048
- Visitors: 2150
- Downloads: 115
Collections
Prinsloo, Earl (Dr)
Edkins, Adrienne Lesley (Associate Prof)
Blatch, Gregory Lloyd (Prof)
RU Department of Biochemistry, Microbiology and Bioinformatics
SDG 09. Industry, Innovation and Infrastructure
SDG 03. Good Health and Well-Being
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details | SOURCE1 | Hsp90αβ associates with the GSK3β.pdf | 689 KB | Adobe Acrobat PDF | View Details |