- Title
- Detection of the in vitro modulation of Plasmodium falciparum Arf1 by Sec7 and ArfGAP domains using a colorimetric plate-based assay:
- Creator
- Swart, Tarryn, Khan, Farrah D, Ntlantsana, Apelele, Laming, Dustin, Veale, Clinton G L, Przyborski, Jude M, Edkins, Adrienne L, Hoppe, Heinrich C
- Date
- 2020
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/165418
- Identifier
- vital:41242
- Identifier
- https://0-doi.org.wam.seals.ac.za/10.1038/s41598-020-61101-3
- Description
- The regulation of human Arf1 GTPase activity by ArfGEFs that stimulate GDP/GTP exchange and ArfGAPs that mediate GTP hydrolysis has attracted attention for the discovery of Arf1 inhibitors as potential anti-cancer agents. The malaria parasite Plasmodium falciparum encodes a Sec7 domain-containing protein - presumably an ArfGEF - and two putative ArfGAPs, as well as an Arf1 homologue (PfArf1) that is essential for blood-stage parasite viability. However, ArfGEF and ArfGAP-mediated activation/deactivation of PfArf1 has not been demonstrated.
- Format
- 12 pages, pdf
- Language
- English
- Relation
- Scientific reports, Swart, T., Khan, F.D., Ntlantsana, A., Laming, D., Veale, C.G., Przyborski, J.M., Edkins, A.L. and Hoppe, H.C., 2020. Detection of the in vitro modulation of Plasmodium falciparum Arf1 by Sec7 and ArfGAP domains using a colorimetric plate-based assay. Scientific reports, 10(1), pp.1-11, Scientific reports volume 10 number 1 1 11 March 2020 2045-2322
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the Nature Terms and Conditions Statement (https://www.nature.com/info/terms-and-conditions)
- Hits: 3180
- Visitors: 3355
- Downloads: 216
Collections
RU DST/NRF SARChI for Molecular and Cellular Biology of the Eukaryotic Stress Response
Edkins, Adrienne Lesley (Associate Prof)
RU Centre for Chemico- and Biomedicinal Research (CCBR)
Hoppe, Heinrich C (Prof)
RU Department of Chemistry
RU Department of Biochemistry, Microbiology and Bioinformatics
SDG 03. Good Health and Well-Being
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | SOURCE1 | Detection of the in vitro modulation of Plasmodium falciparum Arf1.pdf | 1 MB | Adobe Acrobat PDF | View Details Download |