Detection of the in vitro modulation of Plasmodium falciparum Arf1 by Sec7 and ArfGAP domains using a colorimetric plate-based assay:

Title: Detection of the in vitro modulation of Plasmodium falciparum Arf1 by Sec7 and ArfGAP domains using a colorimetric plate-based assay:
Creator: Swart, Tarryn, Khan, Farrah D, Ntlantsana, Apelele, Laming, Dustin, Veale, Clinton G L, Przyborski, Jude M, Edkins, Adrienne L, Hoppe, Heinrich C
Date: 2020
Type: text
Type: article
Identifier: http://hdl.handle.net/10962/165418
Identifier: vital:41242
Identifier: https://0-doi.org.wam.seals.ac.za/10.1038/s41598-020-61101-3
Description: The regulation of human Arf1 GTPase activity by ArfGEFs that stimulate GDP/GTP exchange and ArfGAPs that mediate GTP hydrolysis has attracted attention for the discovery of Arf1 inhibitors as potential anti-cancer agents. The malaria parasite Plasmodium falciparum encodes a Sec7 domain-containing protein - presumably an ArfGEF - and two putative ArfGAPs, as well as an Arf1 homologue (PfArf1) that is essential for blood-stage parasite viability. However, ArfGEF and ArfGAP-mediated activation/deactivation of PfArf1 has not been demonstrated.
Format: 12 pages, pdf
Language: English
Relation: Scientific reports
Rights: Publisher
Rights: Use of this resource is governed by the terms and conditions of the Nature Terms and Conditions Statement (https://www.nature.com/info/terms-and-conditions)

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RU Centre for Chemico- and Biomedicinal Research (CCBR)

RU DST/NRF SARChI for Molecular and Cellular Biology of the Eukaryotic Stress Response

Hoppe, Heinrich C (Prof)

RU Department of Biochemistry, Microbiology and Biotechnology

Edkins, Adrienne Lesley (Associate Prof)

RU Department of Chemistry

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