- Title
- Photodynamic antimicrobial chemotherapy against Staphylococcus aureus and Escherichia coli sensitized using indium (III) cationic porphyrins linked to core-shell magnetic nanoparticles
- Creator
- Makola, Lekgowa Collen
- ThesisAdvisor
- Nyokong, Tebello
- Subject
- Photochemotherapy
- Subject
- Photosensitizing compounds
- Subject
- Staphylococcus aureus
- Subject
- Escherichia coli
- Subject
- Indium
- Subject
- Porphyrins
- Subject
- Magnetic nanoparticles
- Subject
- Quaternize
- Date
- 2021-04
- Type
- thesis
- Type
- text
- Type
- Masters
- Type
- MSc
- Identifier
- http://hdl.handle.net/10962/177225
- Identifier
- vital:42801
- Description
- Photodynamic antimicrobial chemotherapy (PACT) is a well-known antimicrobial therapy technique used against multi-drug resistant pathogens. In this study, the syntheses, characterization, photophysicochemical properties, and the applications of symmetrically and asymmetrically substituted cationic indium (III) porphyrins linked to silver/copper ferrite core-shell (Ag/CuFe2O4) magnetic nanoparticles (MNPs) as potential photosensitizers for PACT are reported. The synthesized complexes include axially modified porphyrins quaternized through an axial ligand. All the asymmetrically substituted porphyrins were linked to the NPs via an ester bond and the symmetrically substituted porphyrins were linked (peripherally and /or axially) via self-assembly (Ag-S and/or Ag-N). The impact of axial modification, peripheral substituents, conjugation to the NPs, the number of positive charges, and the chain length of the alkyl halides quaternizing agents on PACT efficacy and photophysicochemical properties of porphyrins were studied. High singlet oxygen quantum yields and antimicrobial log reductions were observed. Lipophilicity and hydrophilicity of the porphyrins were also studies, where the complexes quaternized with methyl iodide showed relatively high hydrophilicity character. Upon in vitro PACT studies, the quaternized complexes were observed to have 0% viable colony, signifying their effectiveness. Moreover, the highest log reductions of 9.27 were observed against S. aureus and 9.58 were observed against E. coli. The findings from this work delineate that singlet oxygen generation alone is not a distinct factor on the PACT efficacy of the porphyrin complexes, since some of the complexes have practically the same singlet oxygen quantum but different PACT activity. However, other contributing factors including water solubility play a significant role.
- Description
- Thesis (MSc) -- Faculty of Science, Department of Chemistry, 2021
- Format
- computer, online resource, application/pdf, 1 online resource (184 pages), pdf
- Publisher
- Rhodes University, Faculty of Science, Department of Chemistry
- Language
- English
- Rights
- Makola, Lekgowa Collen
- Rights
- All Rights Reserved
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| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | SOURCE1 | MAKOLA-MSC-TR21-59.pdf | 5 MB | Adobe Acrobat PDF | View Details Download |