- Title
- Anisotropic copper oxide nanorods decorated with gold and palladium nanoparticles and their enzymatic properties
- Creator
- Sicwetsha, Simbongile
- ThesisAdvisor
- Mashazi, P
- Subject
- To be added
- Date
- 2021-04
- Type
- thesis
- Type
- text
- Type
- Masters
- Type
- MSc
- Identifier
- http://hdl.handle.net/10962/178547
- Identifier
- vital:42949
- Description
- The synthesis of spherical CuO nanoparticles (CuONPs), copper oxide nanorods (CuONRs), CuONRs decorated with gold (CuONRs@Au1.0NPs), CuONRs decorated with palladium (CuONRs@Pd1.0NPs) and CuONRs decorated with gold and palladium (CuONRs@Au0.5/Pd 0.5NPs) was carried out. The successful preparation of these nanomaterials was confirmed using UV-vis, DLS (zeta potential), XRD, TEM and EDS. The nanoparticles were found to possess intrinsic peroxidase-like activity. The peroxidase-like activity of the nanoparticles was dependent on pH, temperature and enzyme substrate concentration. The investigation of the steady-state kinetic parameters showed that the peroxidase-like activity of the nanomaterials followed the Michaelis-Menten kinetics behaviour. The spherical CuONPs showed the Km = 1.12 mM and 1.14 mM for H2O2 and TMB respectively. The CuONRs showed the Km = 40.04 mM and 2.91 mM for H2O2 and TMB respectively. The CuONRs@Au1.0NPs showed the Km = 3.05 mM and 6.49 mM for H2O2 and TMB respectively. The CuONRs@Pd1.0NPs showed the Km = 0.13 mM and 2.59 mM for H2O2 and TMB respectively. The CuONRs@Au0.5/Pd0.5NPs showed the Km = 2.66 mM and Km = 19.70 mM for H2O2 and TMB respectively. The nanomaterials interact with hydrogen peroxide to produce hydroxyl radicals (OH·). Therefore, the production of reactive oxygen species (ROS) was investigated and detected using 1,3-diphenylisobenzofuran (DPBF) as a radical scavenger. The prepared nanomaterials were used in biosensing for the colorimetric detection of glucose. The LOD and LOQ for spherical CuONPs was 0.73 μM and 2.42 μM, for CuONRs was 0.13 μM and 0.42 μM, CuONRs@Au1.0NPs was 7.19 μM and 21.78 μM, for CuONRs@Pd1.0 NPs was 19.65 μM and 59.54 μM, and for CuONRs@Au0.5/Pd0.5NPs was 10.46 μM and 31.71 μM.
- Description
- Thesis (MSc) -- Faculty of Science, Chemistry, 2021
- Format
- computer, online resource, application/pdf, 1 online resource (228 pages), pdf
- Publisher
- Rhodes University, Faculty of Science, Chemistry
- Language
- English
- Rights
- Sicwetsha, Simbongile
- Rights
- All Rights Reserved
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RU Department of Chemistry
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | SOURCE1 | SICHWETHA-MSC-TR21-51-EMB.pdf | 1 MB | Adobe Acrobat PDF | View Details Download |