- Title
- A stability-indicating high performance liquid chromatographic assay for the determination of orlistat in capsules
- Creator
- Mohammadi, Ali, Haririan, I, Rezanour, Nasrin, Ghiasi, L, Walker, Roderick B
- Subject
- To be catalogued
- Date
- 2006
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/184267
- Identifier
- vital:44195
- Identifier
- xlink:href="https://doi.org/10.1016/j.chroma.2006.03.038"
- Description
- A stability-indicating HPLC method was developed and validated for the quantitative determination of orlistat in capsule dosage forms. An isocratic separation was achieved using a Perfectsil® target ODS-3, 250 mm × 4.6 mm i.d., 5 μm particle size column with a flow rate of 0.7 ml/min and using a UV detector to monitor the eluate at 210 nm. The mobile phase consisted of methanol:acetonitrile:trifluoroacetic acid (82.5:17.5:0.01, v/v/v). The drug was subjected oxidation, hydrolysis, photolysis and heat to apply stress conditions. Complete separation was achieved for the parent compound and all degradation products in an overall analytical run time of approximately 15 min with the parent compound orlistat eluting at approximately 9 min. The method was linear over the concentration range of 0.02–0.75 mg/ml (r = 0.9998) with a limit of detection and quantitation 0.006 and 0.02 mg/ml, respectively. The method has the requisite accuracy, selectivity, sensitivity and precision to assay orlistat in capsules. Degradation products resulting from the stress studies did not interfere with the detection of orlistat and the assay is thus stability-indicating.
- Format
- computer, online resource, application/pdf, 1 online resource (5 pages), pdf
- Publisher
- Elsevier
- Language
- English
- Relation
- Journal of Chromatography A, Mohammadi, A., Haririan, I., Rezanour, N., Ghiasi, L. and Walker, R.B., 2006. A stability-indicating high performance liquid chromatographic assay for the determination of orlistat in capsules. Journal of chromatography A, 1116(1-2), pp.153-157, Journal of Chromatography A volume 1116 number 1 - 2 p.153 2006 0021-9673
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the Elsevier Terms and Conditions Statement (https://www.elsevier.com/legal/elsevier-website-terms-and-conditions)
- Rights
- Closed Access
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Walker, Roderick Bryan (Prof)
RU Department of Pharmacy
SDG 03. Good Health and Well-Being
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details | SOURCE1 | A stability-indicating high performance liquid chromatographic assay for the determination of orlistat in capsules.pdf | 753 KB | Adobe Acrobat PDF | View Details |