- Title
- Development and validation of a stability-indicating method for the quantitation of paclitaxel in pharmaceutical dosage forms
- Creator
- Mohammadi, Ali, Esimaeili, Farnaz, Dinarvand, Rasoul, Atyabi, Fatemeh, Walker, Roderick B
- Subject
- To be catalogued
- Date
- 2009
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/184278
- Identifier
- vital:44196
- Identifier
- xlink:href="https://doi.org/10.1093/chromsci/47.7.599"
- Description
- A simple, rapid stability-indicating isocratic assay has been developed and validated for the determination of Paclitaxel (PTX) in commercial injection formulations. The assay is performed using a Nucleosil RP-18 (5 µm, 250 × 4.0 mm i.d) column protected by a Nucleosil C18 precolumn (5 µm, 4.0 × 4.0 mm i.d.) with a mobile phase of methanol–water (80:20) and UV detection at 230 nm. The method was found to be specific for PTX in the presence of degradation products with an overall analytical run time of ~ 9 min. Accuracy reported as % bias was found to be 0.1–2.5% bias for all samples tested. Intra-assay precision (repeatability) was found to be 0.22–2.65% RSD, while inter-day precision (intermediate precision) was found to be 1.0–3.0% RSD for the samples studied. The calibration curve was found to be linear with the equation y = 29.78x + 7.65, and a linear regression coefficient of 0.9994 over the concentration range 0.05–20 µg/mL. The limits of quantitation and detection were 0.05 and 0.02 µg/mL, respectively. Taxol (30 mg/5 mL), a commercially available dosage form of PTX, was assayed and 100.6–103.6% of the label claim was recovered.
- Format
- computer, online resource, application/pdf, 1 online resource (6 pages), pdf
- Publisher
- Oxford University Press
- Language
- English
- Relation
- Journal of Chromatographic Science, Mohammadi, A., Esmaeili, F., Dinarvand, R., Atyabi, F. and Walker, R.B., 2009. Development and validation of a stability-indicating method for the quantitation of paclitaxel in pharmaceutical dosage forms. Journal of chromatographic science, 47(7), pp.599-604, Journal of Chromatographic Science volume 47 number 7 p.599 2009 1945-239X
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the Oxford University Press https://academic.oup.com/adaptation/pages/Terms_and_Conditions
- Rights
- Open Access
- Hits: 1656
- Visitors: 1756
- Downloads: 109
Collections
Walker, Roderick Bryan (Prof)
RU Department of Pharmacy
SDG 03. Good Health and Well-Being
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | SOURCE1 | Development and validation of a stability-indicating method for the quantitation of paclitaxel in pharmaceutical dosage forms.pdf | 408 KB | Adobe Acrobat PDF | View Details Download |