- Title
- Quantitation of zolpidem in biological fluids by electro-driven microextraction combined with HPLC-UV analysis
- Creator
- Yaripour, Saeid, Mohammadi, Ali, Esfanjani, Isa, Walker, Roderick B, Nojavan, Saeed
- Subject
- To be catalogued
- Date
- 2018
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/184723
- Identifier
- vital:44266
- Identifier
- xlink:href="http://dx.doi.org/10.17179/excli2018-1140"
- Description
- In this study, for the first time, an electro-driven microextraction method named electromembrane extraction combined with a simple high performance liquid chromatography and ultraviolet detection was developed and validated for the quantitation of zolpidem in biological samples. Parameters influencing electromembrane extraction were evaluated and optimized. The membrane consisted of 2-ethylhexanol immobilized in the pores of a hollow fiber. As a driving force, a 150 V electric field was applied to facilitate the analyte migration from the sample matrix to an acceptor solution through a supported liquid membrane. The pHs of donor and acceptor solutions were optimized to 6.0 and 2.0, respectively. The enrichment factor was obtained >75 within 15 minutes. The effect of carbon nanotubes (as solid nano-sorbents) on the membrane performance and EME efficiency was evaluated. The method was linear over the range of 10-1000 ng/mL for zolpidem (R2 >0.9991) with repeatability (%RSD) between 0.3 % and 7.3 % (n = 3). The limits of detection and quantitation were 3 and 10 ng/mL, respectively. The sensitivity of HPLC-UV for the determination of zolpidem was enhanced by electromembrane extraction. Finally, the method was employed for the quantitation of zolpidem in biological samples with relative recoveries in the range of 60-79 %.
- Format
- computer, online resource, application/pdf, 1 online resource (13 pages), pdf
- Publisher
- University of Mainz
- Language
- English
- Relation
- EXCLI Journal, Yaripour, S., Mohammadi, A., Esfanjani, I., Walker, R.B. and Nojavan, S., 2018. Quantitation of zolpidem in biological fluids by electro-driven microextraction combined with HPLC-UV analysis. EXCLI journal, 17, p.349, EXCLI Journal volume 17 p.349 2018 1611-2156
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the Creative Commons CC BY 4.0 License (Attribution 4.0 International) https://creativecommons.org/licenses/by/4.0/
- Rights
- Open Access
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Collections
Walker, Roderick Bryan (Prof)
RU Department of Pharmacy
SDG 03. Good Health and Well-Being
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | SOURCE1 | Quantitation of zolpidem in biological fluids by electro-driven microextraction combined with HPLC-UV analysis.pdf | 463 KB | Adobe Acrobat PDF | View Details Download |