- Title
- In vitro cytotoxic effects of chemical constituents of Euphorbia grandicornis Blanc against breast cancer cells
- Creator
- Kemboi, Douglas, Peter, Xolani, Langat, Moses K, Mhlanga, Richwell, Vukea, Nyeleti, de la Mare, Jo-Anne, Siwe-Noundou, Xavier, Krause, Rui W M, Tembu, Vuyelwa J
- Subject
- To be catalogued
- Date
- 2021
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/191747
- Identifier
- vital:45160
- Identifier
- xlink:href="https://doi.org/10.1016/j.sciaf.2021.e01002"
- Description
- Euphorbia grandicornis Blanc is widely utilized in traditional medicine for a variety of ailments including body pains associated with skin irritations, inflammation, and snake or scorpion bites. Compounds from E. grandicornis were characterized using spectroscopic techniques, NMR, IR, MS, and melting points and alongside the extracts were evaluated for in vitro anticancer activity against several cancer cell lines. The root extract afforded known, β-glutinol (1), β-amyrin (2), 24-methylenetirucalla-8-en-3β-ol (3), tirucalla-8,25-diene-3β,24R-diol (4), stigmasterol (5), sitosterol (6), and hexyl (E)-3-(4-hydroxy-3-methoxyphenyl)-2-propenoate (7) based on their NMR spectroscopic data for the first report in E. grandicornis. The extracts and isolated compounds were evaluated for anticancer activities against hormone receptor-positive breast cancer (MCF-7), triple-negative breast cancer (HCC70), and non-tumorigenic mammary epithelial (MCF-12A) cell lines. The CH2Cl2 extract exhibited potent, cytotoxicity against MCF-7, HCC70, and MCF-12A cells. The aerial extract exhibited IC50 values of 1.03, 0.301, and 1.68 µg/mL, and root extract displayed IC50 values of 0.83, 0.83 and 3.98 µg/mL against MCF-7, HCC70, and MCF-12A cells respectively. The root extract thus showed selectivity for the cancer cell lines over the non-cancerous control cell line (SI = 4.80). Hexyl (E)-3-(4-hydroxy-3-methoxyphenyl)-2-propenoate (7) showed significant activity with IC50 values of 23.41, 29.45 and 27.01 µM against MCF-7, HCC70 and MCF-12A cells respectively, suggesting non-specific cytotoxicity.
- Format
- computer, online resource, application/pdf, 1 online resource (18 pages), pdf
- Publisher
- Elsevier
- Language
- English
- Relation
- Scien American, Magozwi, D.K., Peter, X., Langat, M.K., Mhlanga, R., Vukea, N., de la Mare, J.A., Siwe-Noundou, X., Krause, R.W. and Tembu, V.J., 2021. In vitro cytotoxic effects of chemical constituents of Euphorbia grandicornis Blanc against breast cancer cells. Scientific African, p.e01002, Scien American p e01002 2021 2468-2276
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the Elsevier Terms and Conditions Statement (https://www.elsevier.com/legal/elsevier-website-terms-and-conditions)
- Rights
- Open Access
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Collections
De la Mare, Jo-Anne (Dr)
Krause, Rui Werner Maçedo (Prof)
RU Department of Chemistry
SDG 03. Good Health and Well-Being
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details | SOURCE1 | In vitro cytotoxic effects of chemical constituents of Euphorbiagrandicornis Blanc against breast cancer cells.pdf | 1 MB | Adobe Acrobat PDF | View Details |