- Title
- Design and evaluation of an electrochemical immunosensor for measles serodiagnosis using measles-specific Immunoglobulin G antibodies
- Creator
- Mashazi, Philani N, Vilakazi, Sibulelo, Nyokong, Tebello
- Subject
- To be catalogued
- Date
- 2013
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/241705
- Identifier
- vital:50962
- Identifier
- xlink:href="https://doi.org/10.1016/j.talanta.2013.06.036"
- Description
- The design of electrochemical immunosensors for the detection of measles-specific antibodies is reported. The measles-antigen modified surface was used as an antibody capture surface. The detection of measles-specific IgG antibodies was accomplished using the voltammetric method and horse-radish peroxidase (HRP) labeled secondary antibody (anti-IgG) as a detecting antibody. The potential applications of the designed immunosensor were evaluated in buffer and serum solutions. The immunosensor exhibited good linearity at concentrations less than 100 ng mL−1 with R2=0.997 and the limit of detection of 6.60 ng mL−1 at 3σ. The potential application of the immunosensor was evaluated in the deliberately infected human and newborn calf serum samples with measles-IgG antibody mimicking real-life samples. The designed electrochemical immunosensor could differentiate between infected and un-infected serum samples as higher catalytic currents were obtained for infected serum samples.
- Format
- computer, online resource, application/pdf, 1 online resource (8 pages), pdf
- Publisher
- Elsevier
- Language
- English
- Relation
- Talanta, Mashazi, P., Vilakazi, S. and Nyokong, T., 2013. Design and evaluation of an electrochemical immunosensor for measles serodiagnosis using measles-specific Immunoglobulin G antibodies. Talanta, 115, pp.694-701, Talanta volume 115 p. 694 2013 1873-3573
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the Elsevier Terms and Conditions Statement (https://www.elsevier.com/legal/elsevier-website-terms-and-conditions)
- Rights
- Closed Access
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Collections
Mashazi, Philani N (Associate Prof)
Nyokong, Tebello (Distinguished Professor)
RU DST/NRF SARChI for Medicinal Chemistry and Nanotechnology
RU Department of Chemistry
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