- Title
- An LC-MS-MS method for the determination of cyclizine in human serum
- Creator
- Mohammadi, Ali, Kanfer, Isadore, Sewram, V, Walker, Roderick B
- Date
- 2005
- Type
- Article
- Identifier
- vital:6408
- Identifier
- http://hdl.handle.net/10962/d1006481
- Description
- Cyclizine is a piperazine derivative with anti-emetic activity that is useful in the prevention and treatment of nausea and vomiting associated with motion sickness. A liquid chromatography-tandem mass spectrometry (LC-MS-MS) method is presented for the quantitation of cyclizine in serum. Sample pretreatment involved liquid-liquid extraction of 200 μl of serum with dichloromethane after the addition of 100 μl each of ammonium hydroxide and internal standard solutions. The extracts were analyzed by HPLC on a Luna ® C18 reversed-phase column and an ion-trap mass spectrometer with an electrospray interface. A limit of detection of 1 ng/ml was determined which allowed for the reliable measurement of cyclizine in the serum of human subjects. The method was found to be linear over the calibration range of 2.5-100 ng/ml. The applicability of this method was demonstrated by the analysis of serum obtained from a human volunteer following administration of a single 50 mg cyclizine hydrochloride tablet. The reported method was observed to have the necessary sensitivity, selectivity, precision and accuracy for monitoring cyclizine concentrations in human subjects following oral administration.
- Format
- 10 pages, pdf
- Language
- English
- Relation
- Mohammadi, A. and Kanfer, I. and Sewram, V. and Walker, R.B. (2005) An LC–MS–MS method for the determination of cyclizine in human serum. Journal of Chromatography B, 824 (1/2). pp. 148-152. Available: http://dx.doi.org/10.1016/j.jchromb.2005.07.015
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Walker, Roderick Bryan (Prof)
Kanfer, Isadore (Prof)
RU Department of Pharmacy
SDG 03. Good Health and Well-Being
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