- Title
- High performance liquid chromatographic analysis of oleandomycin in serum and urine
- Creator
- Stubbs, Christopher, Haigh, John M, Kanfer, Isadore
- Date
- 1986
- Type
- text
- Type
- Article
- Identifier
- vital:6429
- Identifier
- http://hdl.handle.net/10962/d1006590
- Description
- The determination of oleandomycin in serum and urine by high-performance liquid chromatography using erythromycin as internal standard is described. The separation was achieved on a reversed-phase C 1 s column employing acetonitrile-0.05 A4 phosphate buffer (30:70), adjusted to pH 7.0, as the mobile phase with UV detection at 200 nm. A solid-phase extraction procedure, combined with a simple phaseseparation step was used prior to chromatographic analysis. Linear calibration curves were obtained in the concentration ranges 0.25-5.0 pg/ml (serum) and 1 .O-25.0 pg/ml (urine). Precise quantitative analysis has been achieved at these levels with relative standard deviations of < 5%.
- Format
- 5 pages, pdf
- Language
- English
- Relation
- Stubbs, C. and Haigh, J.M. and Kanfer, I. (1986) High performance liquid chromatographic analysis of oleandomycin in serum and urine. Journal of Chromatography, 353 . pp. 33-38. Available: http://dx.doi.org/doi:10.1016/S0021-9673(01)87074-...
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