- Title
- Combination of photodynamic antimicrobial chemotherapy and ciprofloxacin to combat S. aureus and E. coli resistant biofilms
- Creator
- Openda, Yolande Ikala, Nyokong, Tebello
- Subject
- To be catalogued
- Date
- 2023
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/360384
- Identifier
- vital:65084
- Identifier
- xlink:href="https://doi.org/10.1016/j.pdpdt.2022.103142"
- Description
- Photodynamic antimicrobial chemotherapy (PACT) coupled with an antibiotic, ciprofloxacin (CIP), was investigated using two indium metallated cationic photosensitizers, a porphyrin (1) and a phthalocyanine (2). Applying PACT followed by the antibiotic treatment led to a remarkable reduction in the biofilm cell survival of two antibiotic-resistant bacterial strains, S. aureus (Gram-positive) and E. coli (Gram-nenative). Treating both bacteria strains with PACT alone showed no significant activity at 32 µM with 15 min irradiation, while CIP alone exhibited a minimum biofilm inhibition concentration (MBIC) at 4 and 8 µg/mL on S. aureus and E. coli, respectively following 24 h incubation. The combined treatment resulted in the complete eradication of the matured biofilms with high log10 reduction values of 7.05 and 7.20 on S. aureus and E. coli, respectively, at low concentrations. It was found that 15 min PACT irradiation of 8 µM of complexes (1 and 2) combined with 2 µg/mL of CIP have a 100% reduction of the resistant S. aureus biofilms. Whereas the total killing of E. coli was obtained when combining 8 µM of complex 1 and 16 µM of complex 2 both combined with 4 µg/mL of CIP.
- Format
- computer, online resource, application/pdf, 1 online resource (10 pages), pdf
- Publisher
- Elsevier
- Language
- English
- Relation
- Photodiagnosis and Photodynamic Therapy, Openda, Y.I. and Nyokong, T., 2023. Combination of photodynamic antimicrobial chemotherapy and ciprofloxacin to combat S. aureus and E. coli resistant biofilms. Photodiagnosis and Photodynamic Therapy, 42, p.103142, Photodiagnosis and Photodynamic Therapy volume 42 p. 103142 2023 1873-1597
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the Elsevier Terms and Conditions Statement (https://www.elsevier.com/legal/elsevier-website-terms-and-conditions)
- Rights
- Closed Access
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Collections
Nyokong, Tebello (Distinguished Professor)
RU DST/NRF SARChI for Medicinal Chemistry and Nanotechnology
RU Department of Chemistry
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | SOURCE1 | Combination of photodynamic antimicrobial chemotherapy and ciprofloxacin.pdf | 821 KB | Adobe Acrobat PDF | View Details Download |