- Title
- Screening of entomopathogenic fungi against citrus mealybug, Plannococcus citri (Hemiptera: Pseudococcidae)
- Creator
- Chartier FitzGerald, Veronique C, Hill, Martin P, Moore, Sean D, Dames, Joanna F
- Subject
- To be catalogued
- Date
- 2016
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/407059
- Identifier
- vital:70333
- Identifier
- xlink:href="https://hdl.handle.net/10520/EJC195093"
- Description
- Planococcus citri (citrus mealybug) is a common and damaging citrus crop pest which has proven difficult to control using conventional methods, such as chemical pesticides and insect growth regulators, particularly late in the citrus growing season. The virulence of two entomopathogenic fungal species was studied in laboratory bioassays against the crawlers and adults of P. citri. Isolates of Metarhizium anisopliae and Beauveria bassiana, collected from citrus orchards in the Eastern Cape Province in South Africa, were verified using and molecular techniques. Mealybug bioassays were performed in 24-well plates. Beauveria bassiana (GAR 17 B3) and M. anisopliae (FCM AR 23 B3) isolates both resulted in 67.5 % mortality of mealybug crawlers and B. bassiana (GB AR 23 13 3) resulted in 64 % crawler mortality with concentrations of 1 x 107 conidia/ml. These three isolates were further tested in multipledose bioassays to determine the median lethal concentration (LC50), which were 5.29 x 105conidia/ml for the M. anisopliae isolate (FCM AR 23 B3), 4.25 x 106 conidia/ml for B. bassiana (GAR 17 B3), and 6.65 x 107 conidia/ml B. bassiana (GB AR 23 13 3) for crawlers, respectively. The results of this study suggested that two isolates (M. anisopliae FCM AR 23 B3 and B. bassiana GAR 17 B3) showed potential for further development as biological control agents against citrus mealybug. Further research would be required to determine their ability to perform under field conditions.
- Format
- computer, online resource, application/pdf, 1 online resource (9 pages), pdf
- Publisher
- Sabinet
- Language
- English
- Relation
- African Entomology, Chartier FitzGerald, V.C., Hill, M.P., Moore, S.D. and Dames, J.F., 2016. Screening of entomopathogenic fungi against citrus mealybug, Plannococcus citri (Hemiptera: Pseudococcidae). African Entomology, 24(2), pp.343-351, African Entomology volume 24 number 2 p. 343 2016 1021-3589
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the Sabinet Terms and Conditions Statement (https://www.sabinet.co.za/terms-conditions)
- Rights
- Closed Access
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Collections
Hill, Martin Patrick (Distinguished Professor)
Dames, Joanna Felicity (Prof)
RU DST/NRF SARChI for Insects in Sustainable Agricultural Ecosystems
RU Department of Biochemistry, Microbiology and Bioinformatics
SDG 15. Life On Land
RU Department of Zoology and Entomology
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | SOURCE1 | Screening of entomopathogenic fungi against citrus mealybug, Plannococcus citri.pdf | 698 KB | Adobe Acrobat PDF | View Details Download |