- Title
- Real-time monitoring of 3T3-L1 preadipocyte differentiation using a commercially available electric cell-substrate impedance sensor system
- Creator
- Kramer, Adam H, Joos-Vandewalle, Julia, Edkins, Adrienne L, Frost, Carminita L, Prinsloo, Earl
- Subject
- To be catalogued
- Date
- 2014
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/431171
- Identifier
- vital:72751
- Identifier
- xlink:href="https://doi.org/10.1016/j.bbrc.2013.12.123"
- Description
- Real-time analysis offers multiple benefits over traditional end point assays. Here, we present a method of monitoring the optimisation of the growth and differentiation of murine 3T3-L1 preadipocytes to adipocytes using the commercially available ACEA xCELLigence Real-Time Cell Analyser Single Plate (RTCA SP) system. Our findings indicate that the ACEA xCELLigence RTCA SP can reproducibly monitor the primary morphological changes in pre- and post-confluent 3T3-L1 fibroblasts induced to differentiate using insulin, dexamethasone, 3-isobutyl-1-methylxanthine and rosiglitazone; and may be a viable primary method of screening compounds for adipogenic factors.
- Format
- computer, online resource, application/pdf, 1 online resource (6 pages), pdf
- Publisher
- Elsevier
- Language
- English
- Relation
- Biochemical and Biophysical Research Communications, Kramer, A.H., Joos-Vandewalle, J., Edkins, A.L., Frost, C.L. and Prinsloo, E., 2014. Real-time monitoring of 3T3-L1 preadipocyte differentiation using a commercially available electric cell-substrate impedance sensor system. Biochemical and biophysical research communications, 443(4), pp.1245-1250, Biochemical and Biophysical Research Communications volume 443 number 4 p. 1245 2014 0006-291X
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the Elsevier Terms and Conditions Statement (https://www.elsevier.com/legal/elsevier-website-terms-and-conditions)
- Rights
- Closed Access
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- Visitors: 227
- Downloads: 13
Collections
Prinsloo, Earl (Dr)
Edkins, Adrienne Lesley (Associate Prof)
RU DST/NRF SARChI for Biotechnology Innovation and Engagement
SDG 03. Good Health and Well-Being
RU Department of Biochemistry, Microbiology and Bioinformatics
| Thumbnail | File | Description | Size | Format | |||
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| View Details | SOURCE1 | Real-time monitoring of 3T3-L1 preadipocyte differentiation using a commercially available electric cell-substrate impedance sensor system.pdf | 776 KB | Adobe Acrobat PDF | View Details |