- Title
- Overexpression, Purification and Characterisation of the Plasmodium falciparum Hsp70-z (PfHsp70-z) Protein
- Creator
- Zininga, Tawanda, Achilonu, Ikechukwu, Hoppe, Heinrich C, Prinsloo, Earl, Dirr, Heinrich W, Shonhai, Addmore
- Subject
- To be catalogued
- Date
- 2015
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/431719
- Identifier
- vital:72799
- Identifier
- xlink:href="https://doi.org/10.1371/journal.pone.0129445"
- Description
- Six Hsp70-like genes are represented on the genome of Plasmodium falciparum. Of these two occur in the cytosol: P. falciparum Hsp70-z (PfHsp70-z) and PfHsp70-1. PfHsp70-1 is a well characterised canonical Hsp70 that facilitates protein quality control and is crucial for the development of malaria parasites. There is very little known about PfHsp70-z. However, PfHsp70-z is known to be essential and is implicated in suppressing aggregation of asparagine-rich proteins of P. falciparum. In addition, its expression at the clinical stage of malaria correlates with disease prognosis. Based on structural evidence PfHsp70-z belongs to the Hsp110 family of proteins. Since Hsp110 proteins have been described as nucleotide exchange factors (NEFs) of their canonical Hsp70 counterparts, it has been speculated that PfHsp70-z may serve as a NEF of PfHsp70-1. In the current study, P. falciparum cells cultured in vitro were subjected to heat stress, triggering the enhanced expression of PfHsp70-z. Biochemical assays conducted using recombinant PfHsp70-z protein demonstrated that the protein is heat stable and possesses ATPase activity. Furthermore, we observed that PfHsp70-z is capable of self-association. The structural-functional features of PfHsp70-z provide further evidence for its role as a chaperone and possible nucleotide exchange factor of PfHsp70-1.
- Format
- computer, online resource, application/pdf, 1 online resource (13 pages), pdf
- Publisher
- PLOSOne
- Language
- English
- Relation
- PLoS One, Zininga T, Achilonu I, Hoppe H, Prinsloo E, Dirr HW, Shonhai A (2015) Overexpression, Purification and Characterisation of the Plasmodium falciparum Hsp70-z (PfHsp70-z) Protein. PLoS ONE 10(6): e0129445, PLoS One volume 10 number 6 p.e0129445 2015 1932-6203
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the PLOSOne Terms of Use Statement ( https://www.plos.org/terms-of-use)
- Rights
- Open Access
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Collections
Prinsloo, Earl (Dr)
RU DST/NRF SARChI for Biotechnology Innovation and Engagement
Hoppe, Heinrich C (Prof)
RU Department of Biochemistry, Microbiology and Bioinformatics
SDG 03. Good Health and Well-Being
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details | SOURCE1 | Overexpression, purification and characterisation of the Plasmodium falciparum Hsp70-z (PfHsp70-z) protein.pdf | 1 MB | Adobe Acrobat PDF | View Details |