- Title
- Supplementary Material Synthesis and biological evaluation of (E)-cinnamic acid,(E)-2-styrylthiazole and (E)-2-[2-(naphthalen-1-yl) vinyl] thiazole derivatives
- Creator
- Olawode, Emmanuel O, Tandlich, Roman, Prinsloo, Earl, Isaacs, Michelle, Hoppe, Heinrich C, Seldon, Ronnett, Warner, Digby F, Steenkamp, Vanessa, Kaye, Perry T
- Subject
- To be catalogued
- Date
- 2016
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/431778
- Identifier
- vital:72803
- Identifier
- xlink:href=" https://www.arkat-usa.org/get-file/59868/"
- Description
- The screening was conducted using multi-well plates which are suited for HeLa cells in the log phase of growth with final cell density > 10 cells/cm. Each experiment normally includes a blank control, containing medium without the cells.28,45 Non-contaminated HeLa cells (6.57 x 105 cells per well) in media were allowed to grow in the incubator under an atmosphere of 5% CO2 at 37 0C for 24 h. To each well was dispensed 200 µL of HeLa culture, containing 6.57 x 105 cells under LabEAir laminar flow hood (Vivid Air, South Africa); 20 µL of resazurin dye (Sigma TOX-8) and test compound (50 µL) were added, which were then incubated in the presence of 5% CO2 at 37 0C for 24 hours in a shaker, to enhance the distribution of the dye. The absorbance of each well was measured with Bio-tek Power Wave X fluorometer (Beijing, China), and increases in fluorescence was monitored at a wavelength of 590 nm, using an excitation wavelength of 560 nm.
- Format
- computer, online resource, application/pdf, 1 online resource (14 pages), pdf
- Publisher
- Arkivoc
- Language
- English
- Relation
- ARKIVOC, Olawode, E.O., Tandlich, R., Prinsloo, E., Isaacs, M., Hoppe, H., Seldon, R., Warner, D.F., Steenkamph, V. and Kayec, P.T., 2016. Supplementary Material Synthesis and biological evaluation of (E)-cinnamic acid,(E)-2-styrylthiazole and (E)-2-[2-(naphthalen-1-yl) vinyl] thiazole derivatives. ARKIVOC, 6, pp.S1-S14, ARKIVOC volume 6 p. S1 2016 1551-7012
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the ARKIVOC Open Access Statement (https://www.arkat-usa.org/about-arkivoc/)
- Rights
- Open Access
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View Details Download | SOURCE1 | Supplementary Material Synthesis and biological evaluation of (E)-cinnamic acid,(E)-2-(naphthalen-1-yl)vinyl]thiazole.pdf | 1 MB | Adobe Acrobat PDF | View Details Download |