- Title
- Unravelling the unfolding mechanism of human integrin linked kinase by GdmCl-induced denaturation
- Creator
- Syed, Sunayana B, Khan, Faez I, Khan, Sabab H, Srivastava, Saurabha, Hasan, Gulam M, Lobb, Kevin A, Islam, Asimul, Hassan, M Imtaiyaz, Ahmad, Faizan
- Subject
- To be catalogued
- Date
- 2018
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/447220
- Identifier
- vital:74593
- Identifier
- xlink:href="https://doi.org/10.1016/j.ijbiomac.2018.06.025"
- Description
- Integrin-linked kinase (ILK) is a ubiquitously expressed Ser/Thr kinase which plays significant role in the cell-matrix interactions and growth factor signalling. In this study, guanidinium chloride (GdmCl)-induced unfolding of kinase domain of ILK (ILK193–446) was carried out at pH 7.5 and 25 °C. Eventually, denaturation curves of mean residue ellipticity at 222 nm ([θ]222) and fluorescence emission spectrum were analysed to estimate stability parameters. The optical properties maximum emission (λmax) and difference absorption coefficient at 292 nm (Δε292) were analysed. The denaturation curve was measured only in the GdmCl molar concentration ranging 3.0–4.2 M because protein was aggregating below 3.0 M of GdmCl concentrations. The denaturation process of ILK193–446 was found as reversible at [GdmCl] ≥ 3.0 M. Moreover, a coincidence of normalized denaturation curves of optical properties ([θ]222, Δε292 and λmax) suggesting that GdmCl-induced denaturation of ILK193–446 is a two-state process. In addition, 100 ns molecular dynamics simulations were performed to see the effects of GdmCl on the structure and stability of ILK193–446. Both the spectroscopic and molecular dynamics ap proaches provided clear insights into the stability and conformational properties of ILK.
- Format
- computer, online resource, application/pdf, 1 online resource (12 pages), pdf
- Publisher
- Elsevier
- Language
- English
- Relation
- International Journal of Biological Macromolecules, Syed, S.B., Khan, F.I., Khan, S.H., Srivastava, S., Hasan, G.M., Lobb, K.A., Islam, A., Hassan, M.I. and Ahmad, F., 2018. Unravelling the unfolding mechanism of human integrin linked kinase by GdmCl-induced denaturation. International journal of biological macromolecules, 117, pp.1252-1263, International Journal of Biological Macromolecules volume 117 p. 1252 2018 0141-8130
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the Elsevier Terms and Conditions Statement (https://www.elsevier.com/legal/elsevier-website-terms-and-conditions)
- Rights
- Closed Access
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Lobb, Kevin Allan (Assoc Prof)
RU Department of Chemistry
SDG 03. Good Health and Well-Being
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