Phytochemical investigation and antidiabetic activity of cissampelos capensis and strychnos henningsii the Eastern Cape medicinal plants
- Authors: Latolla, Nehemiah Solomon
- Date: 2022-04
- Subjects: Medicinal plants -- Eastern Cape (South Africa) , Endemic plants -- South Africa
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/56145 , vital:55956
- Description: Diabetes mellitus is recorded as a significant health crisis in South Africa and various medicinal plants are used for the management of diabetes. However, the chemistry and bioactivity associated with these plants' antidiabetic activity are still lacking. Cissampelos capensis L.f. and Strychnos henningsii Gilg are among the plants utilised to manage diabetes in the Eastern Cape Province, South Africa. C. capensis and S. henningsii are known for their isoquinoline – and indole alkaloids, respectively. Other phytochemical groups associated with antidiabetic activity are the phenols, flavonoids, and terpenes. Thus, this study aimed to evaluate in-depth the phytochemical profiling, alkaloidal identification, and structural characterisation of phytochemicals isolated from these plants. Also, subsequent in vitro antidiabetic screening of the crude extracts and isolated compounds from both plants was done. The phytochemical profiling was performed by employing a qualitative and quantitative approach through high-performance thin layer chromatography (HPTLC) and UV-spectrometry. Further analysis for the identification of alkaloids was done by using LCMS. Fractionation and purification of crude extracts were achieved through crystallisation and various chromatographic techniques. NMR, HRMS, UV/Vis, FTIR, and XRD spectroscopic techniques were used to characterise isolated compounds. In vitro antidiabetic activity screening involved the investigation of cytotoxicity, antioxidant activity, alpha-amylase – and/or alpha-glucosidase inhibition (particular to type II diabetes). C. capensis and S. henningsii extracts tested positive for alkaloids, phenolics, flavonoids, and terpenes through HPTLC screening. Quantitative estimation by UV spectrometry of these phytochemical groups detected the highest concentration of alkaloids, followed by flavonoids, phenolics, and terpenes content. The in vitro antidiabetic biological screening of the various crude extracts suggested that the presence of alkaloids, flavonoids, phenolics, and terpenes fostered favourable biological activity. The LCMS of the known alkaloidal constituents from C. capensis detected the presence of ten alkaloids, including glaziovine, lauroscholtzine, pronuciferine and vi salutardine. Seven known alkaloids and one triterpene were detected from S. henningsii, including holtsiine, 23-hdroxyspermostrychnine, henningsiine, and the triterpene, friedelin. These findings suggest that C. capensis and S. henningsii have similar phytochemical constituents compared to the species reported in literature. However, the distribution of the phytochemicals in the various plant parts differed from previous accounts. , Thesis (PhD) -- Faculty of Science,School of Biomecular and Chemical Sciences , 2022
- Full Text:
- Date Issued: 2022-04
- Authors: Latolla, Nehemiah Solomon
- Date: 2022-04
- Subjects: Medicinal plants -- Eastern Cape (South Africa) , Endemic plants -- South Africa
- Language: English
- Type: Doctoral theses , text
- Identifier: http://hdl.handle.net/10948/56145 , vital:55956
- Description: Diabetes mellitus is recorded as a significant health crisis in South Africa and various medicinal plants are used for the management of diabetes. However, the chemistry and bioactivity associated with these plants' antidiabetic activity are still lacking. Cissampelos capensis L.f. and Strychnos henningsii Gilg are among the plants utilised to manage diabetes in the Eastern Cape Province, South Africa. C. capensis and S. henningsii are known for their isoquinoline – and indole alkaloids, respectively. Other phytochemical groups associated with antidiabetic activity are the phenols, flavonoids, and terpenes. Thus, this study aimed to evaluate in-depth the phytochemical profiling, alkaloidal identification, and structural characterisation of phytochemicals isolated from these plants. Also, subsequent in vitro antidiabetic screening of the crude extracts and isolated compounds from both plants was done. The phytochemical profiling was performed by employing a qualitative and quantitative approach through high-performance thin layer chromatography (HPTLC) and UV-spectrometry. Further analysis for the identification of alkaloids was done by using LCMS. Fractionation and purification of crude extracts were achieved through crystallisation and various chromatographic techniques. NMR, HRMS, UV/Vis, FTIR, and XRD spectroscopic techniques were used to characterise isolated compounds. In vitro antidiabetic activity screening involved the investigation of cytotoxicity, antioxidant activity, alpha-amylase – and/or alpha-glucosidase inhibition (particular to type II diabetes). C. capensis and S. henningsii extracts tested positive for alkaloids, phenolics, flavonoids, and terpenes through HPTLC screening. Quantitative estimation by UV spectrometry of these phytochemical groups detected the highest concentration of alkaloids, followed by flavonoids, phenolics, and terpenes content. The in vitro antidiabetic biological screening of the various crude extracts suggested that the presence of alkaloids, flavonoids, phenolics, and terpenes fostered favourable biological activity. The LCMS of the known alkaloidal constituents from C. capensis detected the presence of ten alkaloids, including glaziovine, lauroscholtzine, pronuciferine and vi salutardine. Seven known alkaloids and one triterpene were detected from S. henningsii, including holtsiine, 23-hdroxyspermostrychnine, henningsiine, and the triterpene, friedelin. These findings suggest that C. capensis and S. henningsii have similar phytochemical constituents compared to the species reported in literature. However, the distribution of the phytochemicals in the various plant parts differed from previous accounts. , Thesis (PhD) -- Faculty of Science,School of Biomecular and Chemical Sciences , 2022
- Full Text:
- Date Issued: 2022-04
The investigation of chemical variation in the stems and rhizomes of cissampelos capensis l. f. (menispermaceae) the Eastern Cape medicinal plant
- Authors: Latolla, Nehemiah Solomon
- Date: 2017
- Subjects: Plants -- Composition Medicinal plants Endemic plants
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: http://hdl.handle.net/10948/13970 , vital:27362
- Description: Cissampelos capensis L. f. (Menispermaceae) commonly known as “Mayisake” (Xhosa) and “Dawidjieswortel” (Afrikaans) is the most commonly used medicinal plant of the Menispermaceae family in South Africa. The rhizomes of Cissampelos capensis are the most used part of the plant to treat illnesses. This poses a threat to the ongoing conservation of this valuable endemic species. Thus, we studied the chemical variation and subsequently the bioactivity in the stems and rhizomes of Cissampelos capensis, the Eastern Cape medicinal plant, to suggest the possible use of the stems for medicinal purposes. We studied the variation in the chemical composition in the stems and the rhizomes by employing four different extraction methods; the methanolic-, non-polar-, alkaloidal- and Total Tertiary Alkaloidal (TTA) to extract compounds from Cissampelos capensis. Employing various chromatographic techniques, HPLC, NMR and LC/MS to investigate the chemical variation and the in vitro assays for the crude materials of the stems and rhizomes of Cissampelos capensis. We successfully documented a library of the phytochemicals content in Cissampelos capensis from three different regions in the Eastern Cape. We could profile different extraction methods, with the TTA extraction delivering the best comparative profile. Employing LC/MS and HPLC we identified eleven known alkaloidal compounds in the stems and rhizomes. We also isolated a known alkaloid, pronuciferine, from the TTA extract and a crystal structure of proto-quericitol from the methanolic extract, which was isolated for the first time from Cissampelos capensis. Preliminary in vitro results suggested anti-inflammatory activity in the methanolic extracts of the stems and rhizomes, while the TTA extracts showed some cytotoxicity. Finally, through analytical HPLC analysis this study found as high as 31% similarity between the chemical variation of the stems and rhizomes, but key alkaloids were not always present, indicating more work is needed before the stems can be recommended as a replacement for the rhizomes.
- Full Text:
- Date Issued: 2017
- Authors: Latolla, Nehemiah Solomon
- Date: 2017
- Subjects: Plants -- Composition Medicinal plants Endemic plants
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: http://hdl.handle.net/10948/13970 , vital:27362
- Description: Cissampelos capensis L. f. (Menispermaceae) commonly known as “Mayisake” (Xhosa) and “Dawidjieswortel” (Afrikaans) is the most commonly used medicinal plant of the Menispermaceae family in South Africa. The rhizomes of Cissampelos capensis are the most used part of the plant to treat illnesses. This poses a threat to the ongoing conservation of this valuable endemic species. Thus, we studied the chemical variation and subsequently the bioactivity in the stems and rhizomes of Cissampelos capensis, the Eastern Cape medicinal plant, to suggest the possible use of the stems for medicinal purposes. We studied the variation in the chemical composition in the stems and the rhizomes by employing four different extraction methods; the methanolic-, non-polar-, alkaloidal- and Total Tertiary Alkaloidal (TTA) to extract compounds from Cissampelos capensis. Employing various chromatographic techniques, HPLC, NMR and LC/MS to investigate the chemical variation and the in vitro assays for the crude materials of the stems and rhizomes of Cissampelos capensis. We successfully documented a library of the phytochemicals content in Cissampelos capensis from three different regions in the Eastern Cape. We could profile different extraction methods, with the TTA extraction delivering the best comparative profile. Employing LC/MS and HPLC we identified eleven known alkaloidal compounds in the stems and rhizomes. We also isolated a known alkaloid, pronuciferine, from the TTA extract and a crystal structure of proto-quericitol from the methanolic extract, which was isolated for the first time from Cissampelos capensis. Preliminary in vitro results suggested anti-inflammatory activity in the methanolic extracts of the stems and rhizomes, while the TTA extracts showed some cytotoxicity. Finally, through analytical HPLC analysis this study found as high as 31% similarity between the chemical variation of the stems and rhizomes, but key alkaloids were not always present, indicating more work is needed before the stems can be recommended as a replacement for the rhizomes.
- Full Text:
- Date Issued: 2017
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