- Title
- Survival of bifidobacteria and their usefulness in faecal source tracking
- Creator
- Luyt, Catherine D, Khamanga, Sandile M, Muller, Wilhelmine J, Tandlich, Roman
- Date
- 2015
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/76117
- Identifier
- vital:30508
- Identifier
- https://doi.org/10.1515/lwr-2015-0001
- Description
- Bifidobacteria have long since been recommended as indicators of human and animal pollution. Concentration ratio (tracking ratio) of the sorbitol-utilising bifidobacteria (SUB) and the total bifidobacteria (TB) can be used to distinguish between animal and human sources of faecal water contamination. The cut-off value needs to be calibrated in a given geographical area. Seven sites with permanent faecal contamination were selected in South Africa. Concentrations of SUB ranged from 10-50000 cells/100 mL, while TB ranged from 0-8000 cells/100 mL. The tracking ratio ranged from 0.10 to 6.25, but no clear cut-off value could be established. The YN-17 agar was replaced for TB with the modified Beerens medium with pH = 5.70, to suppress the growth of faecal streptococci. Tracking ratios observed are most likely the results of different survival rates of SUB and TB. Bifidobacteria die-off due to nutrients was not found to be significant using design of experiment. Thus a lack of continuous input or oxygen levels in water may be major factors. This would limit the ratios used as a faecal source tracking method.
- Format
- Language
- English
- Relation
- Waste Treatment and Recovery, Luyt, C.D., Khamanga, S.M.M., Muller, W.J. and Tandlich, R., 2015. Survival of Bifidobacteria and their usefulness in Faecal Source Tracking. Waste Treatment and Recovery, 1(1)., Waste Treatment and Recovery volume 1 number 1 July 2015 2544-8544
- Rights
- Authors
- Rights
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Muller, Nikite Wilhelmine Josephine (Dr)
Khamanga, Sandile S M (Assoc Prof)
Tandlich, Roman (Dr)
SDG 06. Clean Water and Sanitation
RU Department of Pharmacy
SDG 03. Good Health and Well-Being
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