- Title
- The development of an in vitro system for the production of drug metabolites using microsomal enzymes from bovine liver
- Creator
- Morrison, Roxanne
- ThesisAdvisor
- Wilhelmi, Brendan
- ThesisAdvisor
- Tandlich, Roman
- ThesisAdvisor
- Edkins, Adrienne
- Subject
- Drugs -- Metabolism
- Subject
- Xenobiotics -- Metabolism
- Subject
- Metabolites
- Subject
- Drugs -- Testing
- Subject
- Toxicity testing -- In vitro
- Subject
- Doping in horse racing -- Control -- Research
- Date
- 2011
- Type
- Thesis
- Type
- Masters
- Type
- MSc
- Identifier
- vital:4087
- Identifier
- http://hdl.handle.net/10962/d1007698
- Identifier
- Drugs -- Metabolism
- Identifier
- Xenobiotics -- Metabolism
- Identifier
- Metabolites
- Identifier
- Drugs -- Testing
- Identifier
- Toxicity testing -- In vitro
- Identifier
- Doping in horse racing -- Control -- Research
- Description
- Drug metabolism is a specialised subset of xenobiotic metabolism, pertaining to the breakdown and elimination of pharmaceutical drugs. The enzymes involved in these pathways are the cytochrome P450 family of isozymes. Metabolism is an important factor in determining the pharmacological effects of drugs. The main aim of this study was to develop a system whereby the major metabolites of drugs can be produced in vitro. An in vitro system was developed and optimised using commercially prepared microsomes from rat liver and coumarin (by monitoring its conversion to 7-hydroxycoumarin) as a model. The optimum running conditions for the incubations were 50 μM coumarin, 50 μg protein/ml microsomes, 1 mM NADP⁺, 5 mM G6P and 1U/ml G6PDH incubated for 30 minutes at 38℃. The HPLC method for the detection of coumarin and 7-hydroxycoumarin was also validated with respect to linearity, reproducibility, precision, accuracy and lower limits of detection and quantification. The system developed was then tested using microsomes prepared from fresh bovine liver on these ten drugs of interest in doping control in horse racing: diazepam, nordiazepam, oxazepam, promazine, acepromazine, chlorpromazine, morphine, codeine, etoricoxib and lumiracoxib. The bovine liver microsomes were prepared using differential centrifugation and had activity on a par with the commercial preparations. This in vitro system metabolised the drugs and produced both phase I and II metabolites, similar to those observed in humans and horses in vivo. For example, the major metabolites of the benzodiazepine drug, diazepam, nordiazepam, temazepam and oxazepam as well as the glucuronidated phase II products were all found after incubations with the bovine liver microsomes. The metabolism of the drugs was also investigated in silico using the computational procedure, MetaSite. MetaSite was able to successfully predict known metabolites for most of the drugs studied. Differences were observed from the in vitro incubations and this is most likely due to MetaSite using only human cytochrome P450s for analysis.
- Format
- 109 p., pdf
- Publisher
- Rhodes University, Faculty of Science, Biochemistry, Microbiology and Biotechnology
- Language
- English
- Rights
- Morrison, Roxanne
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RU Department of Biochemistry, Microbiology and Biotechnology (end of 2014)
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