Behavioural activities and chemical composition of fresh leaf essential oil of Plectranthus aegyptiacus from Southwest Nigeria in mice
- Akuegbe, Enimeya Dressman, Oyemitan, Idris Ajayi, Oyedeji, Opeoluwa, Ogunlowo, Ifeoluwa Isaac, Miya, Gugulethu Mathew, Oyedeji, Adebola
- Authors: Akuegbe, Enimeya Dressman , Oyemitan, Idris Ajayi , Oyedeji, Opeoluwa , Ogunlowo, Ifeoluwa Isaac , Miya, Gugulethu Mathew , Oyedeji, Adebola
- Date: 2021-6
- Subjects: Acute toxicity testing , Carvacrol , Essential oil , Behaviour modification , Plectranthus
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/6506 , vital:46434 , ("https://doi.org/10.30574/gscbps.2021.14.2.0030")
- Description: This study determined the chemical composition of essential oil obtained from fresh leaf of Plectranthus aegyptiacus, and evaluated it for novelty-induced behavioural (NIB) and determine its mechanism(s) of action in mice. The oil was hydro-distillated, and analyzed using gas chromatography-mass spectrometry. The effects of the oil (50, 100 and 150 mg/kg; i.p., n=6) on novelty-induced behavioural was assessed using open field test and head dipping on hole board. Probable mechanism(s) were evaluated using antagonists: flumazenil, naloxone and cyproheptadine at 2 mg/kg each, atropine and yohimbine at 5 mg/kg and 1 mg/kg respectively. The LD50 values obtained were 2154 and 490 mg/kg for oral and intraperitoneal routes respectively. The oil (50, 100 and150 mg/kg) significantly (p less 0.05, 0.01 and 0.01) inhibited all NIB and head dips. Flumazenil significantly (p less than 0.05) reversed the effect of the oil on NIB; atropine, naloxone and cyproheptadine significantly (p less than 0.01, 0.01 and 0.001) potentiated inhibitory effect on NIB respectively, while yohimbine showed no significantly effect. The analyzed oil showed 61 compounds, and the major compounds were carvacrol, germacrene-D, p-cymene and [1,1'-Bicyclopentyl]-2,2'-diol. The study concluded that the oil possessed central nervous system depressant activity, which could be mediated mainly through augmentation of GABAergic neurotransmission, while cholinergic-(muscarinic), adrenergic and serotonergic pathways may be involved.
- Full Text:
- Date Issued: 2021-6
- Authors: Akuegbe, Enimeya Dressman , Oyemitan, Idris Ajayi , Oyedeji, Opeoluwa , Ogunlowo, Ifeoluwa Isaac , Miya, Gugulethu Mathew , Oyedeji, Adebola
- Date: 2021-6
- Subjects: Acute toxicity testing , Carvacrol , Essential oil , Behaviour modification , Plectranthus
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/6506 , vital:46434 , ("https://doi.org/10.30574/gscbps.2021.14.2.0030")
- Description: This study determined the chemical composition of essential oil obtained from fresh leaf of Plectranthus aegyptiacus, and evaluated it for novelty-induced behavioural (NIB) and determine its mechanism(s) of action in mice. The oil was hydro-distillated, and analyzed using gas chromatography-mass spectrometry. The effects of the oil (50, 100 and 150 mg/kg; i.p., n=6) on novelty-induced behavioural was assessed using open field test and head dipping on hole board. Probable mechanism(s) were evaluated using antagonists: flumazenil, naloxone and cyproheptadine at 2 mg/kg each, atropine and yohimbine at 5 mg/kg and 1 mg/kg respectively. The LD50 values obtained were 2154 and 490 mg/kg for oral and intraperitoneal routes respectively. The oil (50, 100 and150 mg/kg) significantly (p less 0.05, 0.01 and 0.01) inhibited all NIB and head dips. Flumazenil significantly (p less than 0.05) reversed the effect of the oil on NIB; atropine, naloxone and cyproheptadine significantly (p less than 0.01, 0.01 and 0.001) potentiated inhibitory effect on NIB respectively, while yohimbine showed no significantly effect. The analyzed oil showed 61 compounds, and the major compounds were carvacrol, germacrene-D, p-cymene and [1,1'-Bicyclopentyl]-2,2'-diol. The study concluded that the oil possessed central nervous system depressant activity, which could be mediated mainly through augmentation of GABAergic neurotransmission, while cholinergic-(muscarinic), adrenergic and serotonergic pathways may be involved.
- Full Text:
- Date Issued: 2021-6
Chemical Profiling, Toxicity and Anti-Inflammatory Activities of Essential Oils from Three Grapefruit Cultivars from KwaZulu-Natal in South Africa
- Miya, Gugulethu, Oyedeji, Opeoluwa, Gondwe, Mavuto, Oyedeji, Adebola
- Authors: Miya, Gugulethu , Oyedeji, Opeoluwa , Gondwe, Mavuto , Oyedeji, Adebola
- Date: 2021-6
- Subjects: South Africa Citrus paradisi grapefruit essential oil toxicity anti-inflammatory Article
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/6479 , vital:46408 , ("https://DOI:10.3390/molecules26113387")
- Description: Introduction: This case report discusses the successful use of an improvised bubble continuous positive airway pressure (CPAP) apparatus made using items commonly available in a poorly resourced district hospital. Case presentation: A 64-year-old female with no co-morbidities presented with respiratory failure due to pulmonary tuberculosis and was not accepted into the regional Intensive Care Unit (ICU) on referral. She required 8 days of improvised bubble CPAP to maintain adequate oxygen saturation before weaning and eventual discharge. Discussion: Improvised bubble CPAP is commonly used in neonatal care in developing countries and well described in literature however, there are no reports of its successful use in adult patients. In the absence of access to ICU or other Non-invasive ventilation (NIV) equipment, improvised bubble CPAP may provide some therapeutic benefit.
- Full Text:
- Date Issued: 2021-6
- Authors: Miya, Gugulethu , Oyedeji, Opeoluwa , Gondwe, Mavuto , Oyedeji, Adebola
- Date: 2021-6
- Subjects: South Africa Citrus paradisi grapefruit essential oil toxicity anti-inflammatory Article
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/11260/6479 , vital:46408 , ("https://DOI:10.3390/molecules26113387")
- Description: Introduction: This case report discusses the successful use of an improvised bubble continuous positive airway pressure (CPAP) apparatus made using items commonly available in a poorly resourced district hospital. Case presentation: A 64-year-old female with no co-morbidities presented with respiratory failure due to pulmonary tuberculosis and was not accepted into the regional Intensive Care Unit (ICU) on referral. She required 8 days of improvised bubble CPAP to maintain adequate oxygen saturation before weaning and eventual discharge. Discussion: Improvised bubble CPAP is commonly used in neonatal care in developing countries and well described in literature however, there are no reports of its successful use in adult patients. In the absence of access to ICU or other Non-invasive ventilation (NIV) equipment, improvised bubble CPAP may provide some therapeutic benefit.
- Full Text:
- Date Issued: 2021-6
The Essential Oil of Eucalyptus grandis W. Hill ex Maiden Inhibits Microbial Growth by Inducing Membrane Damage
- Soyingbe, Oluwagbemiga Sewanu, Oyedeji, Adebola, Basson, Albert Kortze, Opoku, Andy Rowland
- Authors: Soyingbe, Oluwagbemiga Sewanu , Oyedeji, Adebola , Basson, Albert Kortze , Opoku, Andy Rowland
- Date: 2013
- Language: English
- Type: text , Article
- Identifier: http://hdl.handle.net/11260/731 , vital:29666
- Description: Eucalyptus grandis is a medicinal plant which has been indicated by Zulu traditional healer in the treatment of respira- tory tract infections, bronchial infections, asthma and cough. The investigation of the essential oil of this plant could help to verify the rationale behind the use of the plant as a cure for these illnesses. Essential oil was hydro-distilled from the fresh leaves and characterised for the chemical constituents and bioactivity. The main constituents of the oil of the E. grandis are α-Pinene (29.69%), p-Cymene (19.89%), 1,8-cineole (12.80%), α-Terpineol (6.48%), Borneol (3.48%) and D-Limonene (3.14%). The essential oil of E. grandis showed high scavenging of DPPH and ABTS radicals, and was active against 13 of the 16 organisms tested with the MIC ranging from 0.625 mg - 5.0 mg/ml; the MBC value ranged from 2.5 mg - 10 mg/ml. The essential oil also inhibited the growth of 7 of the 8 antibiotic resistant bacteria tested, with MIC ranging from 5 mg/ml - 10 mg/ml. The DNA extracted from the affected microorganisms did not show any dam- age however, there was an increase of released cytosolic LDH activity. We conclude that the antibacterial activity of the essential oil was exhibited through cell membrane damage rather than the damage of the DNA. It is apparent that the bioactivity of the essential oil of E. grandis plays an important role in the plants’ use in folk medicine for the treatment of respiratory tract illnesses.
- Full Text:
- Date Issued: 2013
- Authors: Soyingbe, Oluwagbemiga Sewanu , Oyedeji, Adebola , Basson, Albert Kortze , Opoku, Andy Rowland
- Date: 2013
- Language: English
- Type: text , Article
- Identifier: http://hdl.handle.net/11260/731 , vital:29666
- Description: Eucalyptus grandis is a medicinal plant which has been indicated by Zulu traditional healer in the treatment of respira- tory tract infections, bronchial infections, asthma and cough. The investigation of the essential oil of this plant could help to verify the rationale behind the use of the plant as a cure for these illnesses. Essential oil was hydro-distilled from the fresh leaves and characterised for the chemical constituents and bioactivity. The main constituents of the oil of the E. grandis are α-Pinene (29.69%), p-Cymene (19.89%), 1,8-cineole (12.80%), α-Terpineol (6.48%), Borneol (3.48%) and D-Limonene (3.14%). The essential oil of E. grandis showed high scavenging of DPPH and ABTS radicals, and was active against 13 of the 16 organisms tested with the MIC ranging from 0.625 mg - 5.0 mg/ml; the MBC value ranged from 2.5 mg - 10 mg/ml. The essential oil also inhibited the growth of 7 of the 8 antibiotic resistant bacteria tested, with MIC ranging from 5 mg/ml - 10 mg/ml. The DNA extracted from the affected microorganisms did not show any dam- age however, there was an increase of released cytosolic LDH activity. We conclude that the antibacterial activity of the essential oil was exhibited through cell membrane damage rather than the damage of the DNA. It is apparent that the bioactivity of the essential oil of E. grandis plays an important role in the plants’ use in folk medicine for the treatment of respiratory tract illnesses.
- Full Text:
- Date Issued: 2013
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