- Title
- Electrografting of isophthalic acid monolayer and covalent attachment of antibody onto carbon surfaces
- Creator
- Mwanza, Daniel, Phal, Sereilakhena, Nyokong, Tebello, Tesfalidet, Solomon, Mashazi, Philani N
- Subject
- To be catalogued
- Date
- 2021
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/231345
- Identifier
- vital:49879
- Identifier
- xlink:href="https://doi.org/10.1016/j.electacta.2021.139360"
- Description
- In this study, a 5-diazonium isophthalic acid was synthesized and electrografted onto glassy carbon (GCE) and screen-printed carbon (SPCE) electrodes. SPCE was used to demonstrate fabrication of a miniature device and to compare with conventional glassy carbon electrodes. The isophthalic acid (IPA) electrografted thin film was used for the immobilization of anti-methotrexate antibody (Ab) using carbodiimide activation chemistry to form antibody modified surfaces, GCE-IPA-Ab and SPCE-IPA-Ab. The GCE -IPA-Ab and SPCE-IPA-Ab surfaces were used as capacitive biosensors for the detection of methotrexate (MTX) in phosphate buffer (pH 7.4) using capacitive electrochemical impedance spectroscopy (EIS). The EIS data was analyzed using singular value decomposition (SVD). Principal component regression analysis gave R2 values of 0.99 for both the GCE-IPA-Ab and SPCE-IPA-Ab surfaces. The detection limit from the calibration curve of the GCE-IPA-Ab and SPCE-IPA-Ab was calculated to be 7.0 pmol.L−1 and 5.5 pmol.L−1, respectively.
- Format
- computer, online resource, application/pdf, 1 online resource (9 pages), pdf
- Publisher
- Elsevier
- Language
- English
- Relation
- Electrochimica Acta, Mwanza, D., Phal, S., Nyokong, T., Tesfalidet, S. and Mashazi, P., 2021. Electrografting of isophthalic acid monolayer and covalent attachment of antibody onto carbon surfaces: Construction of capacitive biosensor for methotrexate detection. Electrochimica Acta, 398, p.139360, Electrochimica Acta volume 398 p. 139360 2021 0013-4686
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the Elsevier Terms and Conditions Statement (https://www.elsevier.com/legal/elsevier-website-terms-and-conditions)
- Rights
- Closed Access
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Mashazi, Philani N (Associate Prof)
RU DST/NRF SARChI for Medicinal Chemistry and Nanotechnology
Nyokong, Tebello (Distinguished Professor)
RU Department of Chemistry
SDG 03. Good Health and Well-Being
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details | SOURCE1 | Electrografting of isophthalic acid monolayer.pdf | 765 KB | Adobe Acrobat PDF | View Details |