In vitro cytotoxic effects of chemical constituents of Euphorbia grandicornis Blanc against breast cancer cells
- Kemboi, Douglas, Peter, Xolani, Langat, Moses K, Mhlanga, Richwell, Vukea, Nyeleti, de la Mare, Jo-Anne, Siwe-Noundou, Xavier, Krause, Rui W M, Tembu, Vuyelwa J
- Authors: Kemboi, Douglas , Peter, Xolani , Langat, Moses K , Mhlanga, Richwell , Vukea, Nyeleti , de la Mare, Jo-Anne , Siwe-Noundou, Xavier , Krause, Rui W M , Tembu, Vuyelwa J
- Date: 2021
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/191747 , vital:45160 , xlink:href="https://doi.org/10.1016/j.sciaf.2021.e01002"
- Description: Euphorbia grandicornis Blanc is widely utilized in traditional medicine for a variety of ailments including body pains associated with skin irritations, inflammation, and snake or scorpion bites. Compounds from E. grandicornis were characterized using spectroscopic techniques, NMR, IR, MS, and melting points and alongside the extracts were evaluated for in vitro anticancer activity against several cancer cell lines. The root extract afforded known, β-glutinol (1), β-amyrin (2), 24-methylenetirucalla-8-en-3β-ol (3), tirucalla-8,25-diene-3β,24R-diol (4), stigmasterol (5), sitosterol (6), and hexyl (E)-3-(4-hydroxy-3-methoxyphenyl)-2-propenoate (7) based on their NMR spectroscopic data for the first report in E. grandicornis. The extracts and isolated compounds were evaluated for anticancer activities against hormone receptor-positive breast cancer (MCF-7), triple-negative breast cancer (HCC70), and non-tumorigenic mammary epithelial (MCF-12A) cell lines. The CH2Cl2 extract exhibited potent, cytotoxicity against MCF-7, HCC70, and MCF-12A cells. The aerial extract exhibited IC50 values of 1.03, 0.301, and 1.68 µg/mL, and root extract displayed IC50 values of 0.83, 0.83 and 3.98 µg/mL against MCF-7, HCC70, and MCF-12A cells respectively. The root extract thus showed selectivity for the cancer cell lines over the non-cancerous control cell line (SI = 4.80). Hexyl (E)-3-(4-hydroxy-3-methoxyphenyl)-2-propenoate (7) showed significant activity with IC50 values of 23.41, 29.45 and 27.01 µM against MCF-7, HCC70 and MCF-12A cells respectively, suggesting non-specific cytotoxicity.
- Full Text:
- Date Issued: 2021
- Authors: Kemboi, Douglas , Peter, Xolani , Langat, Moses K , Mhlanga, Richwell , Vukea, Nyeleti , de la Mare, Jo-Anne , Siwe-Noundou, Xavier , Krause, Rui W M , Tembu, Vuyelwa J
- Date: 2021
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/191747 , vital:45160 , xlink:href="https://doi.org/10.1016/j.sciaf.2021.e01002"
- Description: Euphorbia grandicornis Blanc is widely utilized in traditional medicine for a variety of ailments including body pains associated with skin irritations, inflammation, and snake or scorpion bites. Compounds from E. grandicornis were characterized using spectroscopic techniques, NMR, IR, MS, and melting points and alongside the extracts were evaluated for in vitro anticancer activity against several cancer cell lines. The root extract afforded known, β-glutinol (1), β-amyrin (2), 24-methylenetirucalla-8-en-3β-ol (3), tirucalla-8,25-diene-3β,24R-diol (4), stigmasterol (5), sitosterol (6), and hexyl (E)-3-(4-hydroxy-3-methoxyphenyl)-2-propenoate (7) based on their NMR spectroscopic data for the first report in E. grandicornis. The extracts and isolated compounds were evaluated for anticancer activities against hormone receptor-positive breast cancer (MCF-7), triple-negative breast cancer (HCC70), and non-tumorigenic mammary epithelial (MCF-12A) cell lines. The CH2Cl2 extract exhibited potent, cytotoxicity against MCF-7, HCC70, and MCF-12A cells. The aerial extract exhibited IC50 values of 1.03, 0.301, and 1.68 µg/mL, and root extract displayed IC50 values of 0.83, 0.83 and 3.98 µg/mL against MCF-7, HCC70, and MCF-12A cells respectively. The root extract thus showed selectivity for the cancer cell lines over the non-cancerous control cell line (SI = 4.80). Hexyl (E)-3-(4-hydroxy-3-methoxyphenyl)-2-propenoate (7) showed significant activity with IC50 values of 23.41, 29.45 and 27.01 µM against MCF-7, HCC70 and MCF-12A cells respectively, suggesting non-specific cytotoxicity.
- Full Text:
- Date Issued: 2021
Cytotoxicity of triterpenoids from Clerodendrum glabrum against triple negative breast cancer cells in vitro:
- Teclegeorgish, Zecarias W, Mokgalaka, Ntebogeng S, Vukea, Nyeleti, de la Mare, Jo-Anne, Tembu, Vuyelwa J
- Authors: Teclegeorgish, Zecarias W , Mokgalaka, Ntebogeng S , Vukea, Nyeleti , de la Mare, Jo-Anne , Tembu, Vuyelwa J
- Date: 2020
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/161511 , vital:40634 , https://doi.org/10.1016/j.sajb.2020.07.009
- Description: Clerodendrum glabrum is an indigenous medicinal plant that is used to treat cough, cold, sore throat and chest complaints. The stem bark of Clerodendrum glabrum afforded four tritepenoids namely, 3β-olean-12-en-3-yl palmitate (β-amyrin palmitate), (1), 3β hydroxy 5-glutinene (glutinol), (2), 3β-lup-20(29)-en-3-palmitate (Lupeol-3-palmitate), (3), 3β-lup-20(29)-en-3-ol (lupeol) (4) and one common phytosterol (stigmasterol) (5). The structures were established on the basis of their spectroscopic analysis. The compounds were screened for cytotoxicity against the HCC70 triple negative breast cancer (TNBC), MCF-7 hormone receptor positive breast cancer and MCF-12A non-cancerous mammary epithelial cell lines.
- Full Text:
- Date Issued: 2020
- Authors: Teclegeorgish, Zecarias W , Mokgalaka, Ntebogeng S , Vukea, Nyeleti , de la Mare, Jo-Anne , Tembu, Vuyelwa J
- Date: 2020
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/161511 , vital:40634 , https://doi.org/10.1016/j.sajb.2020.07.009
- Description: Clerodendrum glabrum is an indigenous medicinal plant that is used to treat cough, cold, sore throat and chest complaints. The stem bark of Clerodendrum glabrum afforded four tritepenoids namely, 3β-olean-12-en-3-yl palmitate (β-amyrin palmitate), (1), 3β hydroxy 5-glutinene (glutinol), (2), 3β-lup-20(29)-en-3-palmitate (Lupeol-3-palmitate), (3), 3β-lup-20(29)-en-3-ol (lupeol) (4) and one common phytosterol (stigmasterol) (5). The structures were established on the basis of their spectroscopic analysis. The compounds were screened for cytotoxicity against the HCC70 triple negative breast cancer (TNBC), MCF-7 hormone receptor positive breast cancer and MCF-12A non-cancerous mammary epithelial cell lines.
- Full Text:
- Date Issued: 2020
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