- Title
- The inhibitory effects of various substrate pre-treatment by-products and wash liquors on mannanolytic enzymes
- Creator
- Malgas, Samkelo, Van Dyk, J Susan, Abboo, Sagaran, Pletschke, Brett I
- Date
- 2016
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/66156
- Identifier
- vital:28911
- Identifier
- https://doi.org/10.1016/j.molcatb.2015.11.014
- Description
- publisher version
- Description
- Biomass pre-treatment is essential for achieving high levels of bioconversion through increased accessibility of hydrolytic enzymes to hydrolysable carbohydrates. However, pre-treatment by-products, such as sugar and lignin degradation products, can negatively affect the performance of hydrolytic (mannanolytic) enzymes. In this study, two monomeric sugars, five sugar degradation products, five lignin derivatives and four liquors from biomass feedstocks pre-treated by different technologies, were evaluated for their inhibitory effects on mannanolytic enzymes (α-galactosidases, β-mannanases and β-mannosidases). Lignin derivatives elicited the greatest inhibitory effect on the mannanolytic enzymes, followed by organic acids and furan derivatives derived from sugar degradation. Lignin derivative inhibition appeared to be as a result of protein–phenolic complexation, leading to protein precipitating out of solution. The functional groups on the phenolic lignin derivatives appeared to be directly related to the ability of the phenolic to interfere with enzyme activity, with the phenolic containing the highest hydroxyl group content exhibiting the greatest inhibition. It was also demonstrated that various pre-treatment technologies render different pre-treatment soluble by-products which interact in various ways with the mannanolytic enzymes. The different types of biomass (i.e. different plant species) were also shown to release different by-products that interacted with the mannanolytic enzymes in a diverse manner even when the biomass was pre-treated using the same technology. Enzyme inhibition by pre-treatment by-products can be alleviated through the removal of these compounds prior to enzymatic hydrolysis to maximize enzyme activity.
- Format
- 9 pages, pdf
- Language
- English
- Relation
- Journal of Molecular Catalysis B: Enzymatic, Malgas, Samkelo, Van Dyk, J. Susan, Abboo, Sagaran, Pletschke, Brett I. (2016) The inhibitory effects of various substrate pre-treatment by-products and wash liquors on mannanolytic enzymes. Journal of Molecular Catalysis B: Enzymatic, 123, p 132 – 140. SN - 1381-1177. https://doi.org/10.1016/j.molcatb.2015.11.014, Journal of Molecular Catalysis B: Enzymatic volume 123 number 132 140 January 2016 1381-1177
- Rights
- Elsevier B.V.
- Rights
- Use of this resource is governed by the terms and conditions of the Elsevier Copyright Statement (https://www.elsevier.com/about/our-business/policies/copyright)
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Abboo, Sagaran (Dr)
Pletschke, Brett (Prof)
RU Department of Biochemistry, Microbiology and Bioinformatics
SDG 15. Life On Land
SDG 03. Good Health and Well-Being
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