- Title
- In Vitro Studies on Antioxidant and AntiParasitic Activities of Compounds Isolated from Rauvolfia caffra Sond
- Creator
- Tlhapi, Dorcas B, Ramaite, Isaiah D, Anokwuru, Chinedu P, van Ree, Teunis, Hoppe, Heinrich C
- Subject
- To be catalogued
- Date
- 2020
- Type
- text
- Type
- article
- Identifier
- http://hdl.handle.net/10962/425993
- Identifier
- vital:72305
- Identifier
- xlink:href="https://doi.org/10.3390/molecules25173781"
- Description
- As part of an ongoing study of natural products from local medicinal plants, the methanol extract of stem bark of Rauvolfia caffra Sond was investigated for biological activity. Column chromatography and preparative thin-layer chromatography were used to isolate lupeol (1), raucaffricine (2), N-methylsarpagine (3), and spegatrine (4). The crude extract, fractions and isolated compounds were tested for anti-oxidant, antitrypanosomal and anti-proliferation activities. Two fractions displayed high DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging activity and reducing power with IC50 (The half maximal inhibitory concentration) and IC0.5 values of 0.022 ± 0.003 mg/mL and 0.036 ± 0.007 mg/mL, and 0.518 ± 0.044 mg/mL and 1.076 ± 0.136 mg/mL, respectively. Spegatrine (4) was identified as the main antioxidant compound in R. caffra with IC50 and IC0.5 values of 0.119 ± 0.067 mg/mL and 0.712 ± 0 mg/mL, respectively. One fraction displayed high antitrypanosomal activity with an IC50 value of 18.50 μg/mL. However, the major constituent of this fraction, raucaffricine (2), was not active. The crude extract, fractions and pure compounds did not display any cytotoxic effect at a concentration of 50 μg/mL against HeLa cells. This study shows directions for further in vitro studies on the antioxidant and antitrypanosomal activities of Rauvolfia caffra Sond.
- Format
- computer, online resource, application/pdf, 1 online resource (10 pages), pdf
- Publisher
- MDPI
- Language
- English
- Relation
- Molecules, Tlhapi, D.B., Ramaite, I.D.I., Anokwuru, C.P., Van Ree, T. and Hoppe, H.C. (2020) In Vitro Studies on Antioxidant and AntiParasitic Activities of Compounds Isolated from Rauvolfia caffra Sond. Molecules. 25: 3781, Molecules volume 25 p. 3781 2020 1420-3049
- Rights
- Publisher
- Rights
- Use of this resource is governed by the terms and conditions of the MDPI Open Access Statement (https://www.int-res.com/journals/terms-of-use/)
- Rights
- Open Access
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Collections
Hoppe, Heinrich C (Prof)
RU Department of Biochemistry, Microbiology and Bioinformatics
SDG 15. Life On Land
SDG 03. Good Health and Well-Being
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details | SOURCE1 | In Vitro Studies on Antioxidant and AntiParasitic Activities of Compounds Isolated from Rauvolfia caffra Sond.pdf | 1 MB | Adobe Acrobat PDF | View Details |