A multidisciplinary study to assess the ecology of the Cape sea urchin, Parechinus angulosus, and its emerging use as a bioindicator to monitor coastal resiliency
- Authors: Redelinghuys, Suzanne
- Date: 2024-04-05
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/435691 , vital:73179 , DOI 10.21504/10962/435692
- Description: The resilience of marine species in the face of unpredictable climate change stands as a paramount concern for the maintenance of ecological stability. Under such fluctuating conditions, it is critical to understand how organisms mitigate these effects in physiological, genetic, and morphological terms. To that end, this thesis focused on the Cape sea urchin, Parechinus angulosus, employing a multidisciplinary approach encompassing morphology, genomics, and gut microbial diversity to assess its potential as a bioindicator species and elucidate its adaptive strategies in response to varying environmental conditions along the South African coastline. This was achieved through studying their anatomy in order to link observed variation to prevailing local environmental conditions, aided by the species’ wide distribution range which allows insight into adaptations across broad geographic regions and ecological settings. The first empirical chapter, Chapter 3, focusing on eight key morphometric traits of test, Aristotle’s lantern and spines, revealed distinct variation in the Cape sea urchin's morphology between the east and west coasts of South Africa, suggesting the presence of some level of local adaptation to the prevailing environmental factors found on the east and west coasts of South Africa. This points to potential bioindicator capabilities of the species, reflecting adaptive divergence amidst contrasting environmental conditions. Further analysis is however necessary in order to isolate specific physiological trends that may be associated with these morphometric differences, thereby enhancing and tightening their ecological implications. The second experimental chapter, Chapter 4, delved into the genetic structure of the Cape urchin by investigating genome-wide diversity, the presence of cryptic population structure, and spatial patterns of genomic diversity. Moderate genomic differentiation was detected among populations along the eastern and western coasts of South Africa by outlier loci that may undergo natural selection, which could indicate local adaptation to environmental conditions. This pattern hints at adaptive differentiation and cryptic genetic structures within the Cape sea urchin populations and emphasises the species' potential adaptive responses to localised (in this case regional) environmental pressures. Assigning functional significance to these genetic variations will require a comprehensive annotated reference genome, a limitation acknowledged in the current study. Chapter 5 explored the gut microbial diversity and revealed significant compositional variations between the east and west coast populations of South Africa, confirming regional and inter-regional variation. This chapter also highlighted the essential biochemical pathways critical to the survival of the host which is crucial for assessing the health of the urchin host. Together, the functional content of the gut bacteria and microbial diversity showcases its potential as a bioindicator for coastal ecosystem health. Logistical challenges and confounding factors like host physiology will need to be fully considered for its effective application. Overall, the findings of this doctoral research suggest that the Cape sea urchin displays promising characteristics as a bioindicator species due to its morphological, genetic, and gut microbial variations in response to environmental differences, providing a diverse array of means in which urchins could be used as bioindicators, from their uses to assess water quality and detect pollution, to ecosystem health monitoring and biodiversity studies in which sea urchin abundance, distribution, and presence are monitored. Further research, integrating these multidisciplinary approaches is recommended to validate and refine its bioindicator potential. Additionally, the development of a comprehensive annotated reference genome is imperative to harness the species' genetic information effectively. This study underscores the significance of integrating multiple disciplines in understanding how species respond to environmental change and their potentials contributions to monitor ecological resilience. The original multidisciplinary approach, combined with high computational outputs presents a promising framework for a comprehensive ecological monitoring in marine ecosystems. , Thesis (PhD) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-05
- Authors: Redelinghuys, Suzanne
- Date: 2024-04-05
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/435691 , vital:73179 , DOI 10.21504/10962/435692
- Description: The resilience of marine species in the face of unpredictable climate change stands as a paramount concern for the maintenance of ecological stability. Under such fluctuating conditions, it is critical to understand how organisms mitigate these effects in physiological, genetic, and morphological terms. To that end, this thesis focused on the Cape sea urchin, Parechinus angulosus, employing a multidisciplinary approach encompassing morphology, genomics, and gut microbial diversity to assess its potential as a bioindicator species and elucidate its adaptive strategies in response to varying environmental conditions along the South African coastline. This was achieved through studying their anatomy in order to link observed variation to prevailing local environmental conditions, aided by the species’ wide distribution range which allows insight into adaptations across broad geographic regions and ecological settings. The first empirical chapter, Chapter 3, focusing on eight key morphometric traits of test, Aristotle’s lantern and spines, revealed distinct variation in the Cape sea urchin's morphology between the east and west coasts of South Africa, suggesting the presence of some level of local adaptation to the prevailing environmental factors found on the east and west coasts of South Africa. This points to potential bioindicator capabilities of the species, reflecting adaptive divergence amidst contrasting environmental conditions. Further analysis is however necessary in order to isolate specific physiological trends that may be associated with these morphometric differences, thereby enhancing and tightening their ecological implications. The second experimental chapter, Chapter 4, delved into the genetic structure of the Cape urchin by investigating genome-wide diversity, the presence of cryptic population structure, and spatial patterns of genomic diversity. Moderate genomic differentiation was detected among populations along the eastern and western coasts of South Africa by outlier loci that may undergo natural selection, which could indicate local adaptation to environmental conditions. This pattern hints at adaptive differentiation and cryptic genetic structures within the Cape sea urchin populations and emphasises the species' potential adaptive responses to localised (in this case regional) environmental pressures. Assigning functional significance to these genetic variations will require a comprehensive annotated reference genome, a limitation acknowledged in the current study. Chapter 5 explored the gut microbial diversity and revealed significant compositional variations between the east and west coast populations of South Africa, confirming regional and inter-regional variation. This chapter also highlighted the essential biochemical pathways critical to the survival of the host which is crucial for assessing the health of the urchin host. Together, the functional content of the gut bacteria and microbial diversity showcases its potential as a bioindicator for coastal ecosystem health. Logistical challenges and confounding factors like host physiology will need to be fully considered for its effective application. Overall, the findings of this doctoral research suggest that the Cape sea urchin displays promising characteristics as a bioindicator species due to its morphological, genetic, and gut microbial variations in response to environmental differences, providing a diverse array of means in which urchins could be used as bioindicators, from their uses to assess water quality and detect pollution, to ecosystem health monitoring and biodiversity studies in which sea urchin abundance, distribution, and presence are monitored. Further research, integrating these multidisciplinary approaches is recommended to validate and refine its bioindicator potential. Additionally, the development of a comprehensive annotated reference genome is imperative to harness the species' genetic information effectively. This study underscores the significance of integrating multiple disciplines in understanding how species respond to environmental change and their potentials contributions to monitor ecological resilience. The original multidisciplinary approach, combined with high computational outputs presents a promising framework for a comprehensive ecological monitoring in marine ecosystems. , Thesis (PhD) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-05
The influence of the physical environment on invertebrate larval transport, settlement and recruitment with insights on early-stage physiological performance
- Authors: Duna, Oliver Olwethu
- Date: 2024-04-05
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/435663 , vital:73177 , DOI 10.21504/10962/435663
- Description: Most marine benthic organisms have a biphasic life cycle whereby the larvae are pelagic, and their distribution, transport and settlement are largely controlled by environmental factors. Among these factors, hydrodynamics have key direct effects. Nearshore water hydrodynamics are strongly influenced by wind. This study investigated whether wind indirectly affects the distribution, settlement and recruitment of invertebrate marine larvae. Additionally, larvae are exposed to a more constant, benign environment than they will experience after settlement, and therefore the possible effects of larval thermal history on the physiological plasticity of settled organisms was also estimated for possible long-term insights into population dynamics. Four sites, two on the western and two on the eastern side of Algoa Bay in Gqeberha (formerly known as Port Elizabeth), South Africa, were chosen for larval collection. Sites at opposite ends of the bay were selected as they were towards either side of the dominant wind directions in this region. At each site, two sampling stations were identified at 300 and 900 metres offshore, with station replicates 300 metres apart. Sampling of marine invertebrate larvae was conducted after two days of westerly or easterly winds at either of the two western or eastern sites. The number of hours of unidirectional wind determined the prevalent wind on the two days prior to sampling. Samples were collected with a plankton pump at three depths; surface, mid-depth and bottom. Simultaneously with plankton sampling, water properties (fluorescence, temperature, zonal and meridional flow, salinity and oxygen) were measured. Settlement/recruitment samples of mussels and barnacles were collected at six sites, two at either edge of the bay and two within the center of the bay. Plastic scouring pads and PVC plastic plates covered with safety-walk, placed at least 30 cm from each other, were used to collect settlers/recruits of mussels and barnacles, respectively. The pads and plates were replaced on a monthly basis for 14 months to quantify monthly settlement/recruitment. At four of the six sites used for quantifying settlement/recruitment, settlers and recruits were collected to measure the physiological responses of these two ontogenetic stages (settlers and recruits) to acute temperature variations which they might experience in the intertidal zone. Three temperature loggers were used to record real time temperature variation at each site over the period (one month) in which the artificial collectors were in position. Factorial ANOVA was used to investigate the effects of wind, depth, site, and distance from the shore on the abundance of larvae in the water column, as well as the effects of month and site on settlement/recruitment. Additionally, multiple regression analyses were used to investigate the effects of physical parameters, including upwelling and dissipation of turbulent kinetic energy, on the abundance of both larvae, settlers and recruits. Distance-based linear models and redundancy analyses were also carried out on the abundances of invertebrate larvae. Correlation analyses were performed to investigate the effect of the number of hours of directional wind on settlement. Lastly, correlation analyses between wind and currents were carried out. The results revealed a link between winds and currents, with wind speed directly proportional to surface current speed, which, as expected, decreased with depth. Current direction was, however, not perfectly aligned with wind direction. Larval distribution, settlement and recruitment were largely associated with the nearshore dissipation of turbulent kinetic energy and upwelling. Thus, overall larval, settler and recruit abundances were indirectly affected by wind. Physiologically, there was no significant difference in oxygen consumption between the juvenile ontogenetic stages (settlers and recruits) of mussels. Recruits exposed to average and maximum temperatures, however, consumed more oxygen than those exposed to low temperatures. Wind and wind-mediated currents as well as temperature have been shown to shape the distribution and shore supply of larvae, and this study contributes to the broad knowledge of population dynamics and replenishment, and the tight relation of abiotic factors affecting biological processes on the shore. , Thesis (PhD) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-05
- Authors: Duna, Oliver Olwethu
- Date: 2024-04-05
- Subjects: Uncatalogued
- Language: English
- Type: Academic theses , Doctoral theses , text
- Identifier: http://hdl.handle.net/10962/435663 , vital:73177 , DOI 10.21504/10962/435663
- Description: Most marine benthic organisms have a biphasic life cycle whereby the larvae are pelagic, and their distribution, transport and settlement are largely controlled by environmental factors. Among these factors, hydrodynamics have key direct effects. Nearshore water hydrodynamics are strongly influenced by wind. This study investigated whether wind indirectly affects the distribution, settlement and recruitment of invertebrate marine larvae. Additionally, larvae are exposed to a more constant, benign environment than they will experience after settlement, and therefore the possible effects of larval thermal history on the physiological plasticity of settled organisms was also estimated for possible long-term insights into population dynamics. Four sites, two on the western and two on the eastern side of Algoa Bay in Gqeberha (formerly known as Port Elizabeth), South Africa, were chosen for larval collection. Sites at opposite ends of the bay were selected as they were towards either side of the dominant wind directions in this region. At each site, two sampling stations were identified at 300 and 900 metres offshore, with station replicates 300 metres apart. Sampling of marine invertebrate larvae was conducted after two days of westerly or easterly winds at either of the two western or eastern sites. The number of hours of unidirectional wind determined the prevalent wind on the two days prior to sampling. Samples were collected with a plankton pump at three depths; surface, mid-depth and bottom. Simultaneously with plankton sampling, water properties (fluorescence, temperature, zonal and meridional flow, salinity and oxygen) were measured. Settlement/recruitment samples of mussels and barnacles were collected at six sites, two at either edge of the bay and two within the center of the bay. Plastic scouring pads and PVC plastic plates covered with safety-walk, placed at least 30 cm from each other, were used to collect settlers/recruits of mussels and barnacles, respectively. The pads and plates were replaced on a monthly basis for 14 months to quantify monthly settlement/recruitment. At four of the six sites used for quantifying settlement/recruitment, settlers and recruits were collected to measure the physiological responses of these two ontogenetic stages (settlers and recruits) to acute temperature variations which they might experience in the intertidal zone. Three temperature loggers were used to record real time temperature variation at each site over the period (one month) in which the artificial collectors were in position. Factorial ANOVA was used to investigate the effects of wind, depth, site, and distance from the shore on the abundance of larvae in the water column, as well as the effects of month and site on settlement/recruitment. Additionally, multiple regression analyses were used to investigate the effects of physical parameters, including upwelling and dissipation of turbulent kinetic energy, on the abundance of both larvae, settlers and recruits. Distance-based linear models and redundancy analyses were also carried out on the abundances of invertebrate larvae. Correlation analyses were performed to investigate the effect of the number of hours of directional wind on settlement. Lastly, correlation analyses between wind and currents were carried out. The results revealed a link between winds and currents, with wind speed directly proportional to surface current speed, which, as expected, decreased with depth. Current direction was, however, not perfectly aligned with wind direction. Larval distribution, settlement and recruitment were largely associated with the nearshore dissipation of turbulent kinetic energy and upwelling. Thus, overall larval, settler and recruit abundances were indirectly affected by wind. Physiologically, there was no significant difference in oxygen consumption between the juvenile ontogenetic stages (settlers and recruits) of mussels. Recruits exposed to average and maximum temperatures, however, consumed more oxygen than those exposed to low temperatures. Wind and wind-mediated currents as well as temperature have been shown to shape the distribution and shore supply of larvae, and this study contributes to the broad knowledge of population dynamics and replenishment, and the tight relation of abiotic factors affecting biological processes on the shore. , Thesis (PhD) -- Faculty of Science, Zoology and Entomology, 2024
- Full Text:
- Date Issued: 2024-04-05
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