Using four different methods to reach a taxonomic conclusion in dung beetles (Scarabaeinae)
- Authors: Deschodt, Christian Michel
- Date: 2019
- Subjects: Dung beetles -- Classification , Scarabaeidae -- Classification
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/67845 , vital:29156
- Description: Four different techniques to make taxonomic decisions concerning different species in Scarabaeinae are being investigated. Firstly, I made measurements of the body dimensions which are plotted on a two dimensional graph. This method is successfully used to erect one new species, Copris crassus Deschodt and Davis, 2015, and to establish the synonymy of Copris bihamatus Balthasar, 1965 with Copris fidius (Olivier, 1789). Thereafter the classical or traditional comparative method is used to propose seven new species Scarabaeolus soutpansbergensis (Deschodt and Davis 2015), Scarabaeolus megaparvulus (Davis and Deschodt 2015), Scarabaeolus niemandi (Deschodt and Davis 2015), Scarabaeolus carniphilus (Davis and Deschodt 2015), Scarabaeolus ermienae (Deschodt and Davis 2015), Scarabaeolus planipennis (Davis and Deschodt 2015) and Scarabaeolus afronitidus (Davis and Deschodt 2015) and formally synonymise Scarabaeolus vansoni (Ferreira, 1958) with Scarabaeolus lucidulus (Boheman, 1860) and Scarabaeolus xavieri (Ferreira, 1968) with Scarabaeolus andreaei (zur Strassen, 1963). Morphometric measurements of external structures of a group of flightless relict beetles in the tribe Canthonini are used to compile a nexus file which is analysed with computer software. The interpretation of these results is used here to support the erection of a new genus Drogo Deschodt, Davis & Scholtz 2016, Lastly I analysed the DNA sequences of specimens from different species belonging to a species complex in the genus Epirinus Reiche, 1841 occurring over a wide geographic range. These sequences are used together with external morphological characters to propose the synonymy of Epirinus hluhluwensis Medina & Scholtz 2005 and Epirinus ngomae Medina & Scholtz 2005 with Epirinus davisi Scholtz & Howden 1987.
- Full Text:
- Authors: Deschodt, Christian Michel
- Date: 2019
- Subjects: Dung beetles -- Classification , Scarabaeidae -- Classification
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/67845 , vital:29156
- Description: Four different techniques to make taxonomic decisions concerning different species in Scarabaeinae are being investigated. Firstly, I made measurements of the body dimensions which are plotted on a two dimensional graph. This method is successfully used to erect one new species, Copris crassus Deschodt and Davis, 2015, and to establish the synonymy of Copris bihamatus Balthasar, 1965 with Copris fidius (Olivier, 1789). Thereafter the classical or traditional comparative method is used to propose seven new species Scarabaeolus soutpansbergensis (Deschodt and Davis 2015), Scarabaeolus megaparvulus (Davis and Deschodt 2015), Scarabaeolus niemandi (Deschodt and Davis 2015), Scarabaeolus carniphilus (Davis and Deschodt 2015), Scarabaeolus ermienae (Deschodt and Davis 2015), Scarabaeolus planipennis (Davis and Deschodt 2015) and Scarabaeolus afronitidus (Davis and Deschodt 2015) and formally synonymise Scarabaeolus vansoni (Ferreira, 1958) with Scarabaeolus lucidulus (Boheman, 1860) and Scarabaeolus xavieri (Ferreira, 1968) with Scarabaeolus andreaei (zur Strassen, 1963). Morphometric measurements of external structures of a group of flightless relict beetles in the tribe Canthonini are used to compile a nexus file which is analysed with computer software. The interpretation of these results is used here to support the erection of a new genus Drogo Deschodt, Davis & Scholtz 2016, Lastly I analysed the DNA sequences of specimens from different species belonging to a species complex in the genus Epirinus Reiche, 1841 occurring over a wide geographic range. These sequences are used together with external morphological characters to propose the synonymy of Epirinus hluhluwensis Medina & Scholtz 2005 and Epirinus ngomae Medina & Scholtz 2005 with Epirinus davisi Scholtz & Howden 1987.
- Full Text:
Biotic and abiotic drivers of macroinvertebrate assemblages in a South African river
- Authors: Bellingan, Terence Andrew
- Date: 2018
- Subjects: Aquatic insects -- South Africa -- Eastern Cape , Stream ecology -- South Africa -- Eastern Cape , Freshwater ecology -- South Africa -- Eastern Cape , Riparian areas -- Management , Ecosystem management -- South Africa -- Eastern Cape , Mayflies -- South Africa -- Eastern Cape , Stoneflies -- South Africa -- Eastern Cape , Keiskamma River
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/61839 , vital:28067
- Description: Aquatic insects are the most numerically abundant and diverse group of organisms found in lotic ecosystems in South Africa and the world over. They play vital roles in freshwater ecosystem functioning, processing nutrients and in turn forming integral links in stream food-webs. This thesis focussed on examining the macroinvertebrate fauna within three reaches of headwater streams of the Keiskamma River system: reaches that were considered to be fishless; reaches that were invaded by non-native salmonid species; and reaches that were dominated by native fish. I described the effects of predatory fish presence through detailed examination of macroinvertebrate assemblage composition; macroinvertebrate drift timing and density; and through niche utilisation determined from stable isotope data. Patterns in the macroinvertebrate assemblages of the headwaters of the Keiskamma River appear to be driven more strongly by flow rate and seasonal influences, but fish presence and biotope availability were also significant drivers. Niche shifts due to predator presence were not easy to detect and, while patterns of influence may have been evident, they were not found to be significant. However, I demonstrated that salmonids selectively feed on native fish species when the opportunity is presented, occupying significantly higher trophic levels when co-occurring with native fish than in invaded reaches where native fish are absent. Drift timing and density were demonstrated to be significantly different between reach for specific macroinvertebrate species from the Ephemeroptera and Plecoptera, under differing fish predation regimes, in agreement with what has been observed from studies in rivers elsewhere. In freshwater ecosystems of South Africa and worldwide, mitigation of negative effects of alien fishes through their removal using piscicides may also affect non-target organisms. To better understand the effects of just such a removal operation, employed for the first time in the history of freshwater conservation in South Africa, macroinvertebrate communities were assessed for non-target effects of rotenone. The fish eradication operations were demonstrated to have a short-term negative effect on the macroinvertebrate assemblage, through water quality index measurements and alteration of densities of macroinvertebrate taxa collected from stone surfaces. However, no long-term detrimental impact was observed as macroinvertebrate faunas returned to a comparable pre-treatment state within a year of each rotenone application.
- Full Text:
- Authors: Bellingan, Terence Andrew
- Date: 2018
- Subjects: Aquatic insects -- South Africa -- Eastern Cape , Stream ecology -- South Africa -- Eastern Cape , Freshwater ecology -- South Africa -- Eastern Cape , Riparian areas -- Management , Ecosystem management -- South Africa -- Eastern Cape , Mayflies -- South Africa -- Eastern Cape , Stoneflies -- South Africa -- Eastern Cape , Keiskamma River
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/61839 , vital:28067
- Description: Aquatic insects are the most numerically abundant and diverse group of organisms found in lotic ecosystems in South Africa and the world over. They play vital roles in freshwater ecosystem functioning, processing nutrients and in turn forming integral links in stream food-webs. This thesis focussed on examining the macroinvertebrate fauna within three reaches of headwater streams of the Keiskamma River system: reaches that were considered to be fishless; reaches that were invaded by non-native salmonid species; and reaches that were dominated by native fish. I described the effects of predatory fish presence through detailed examination of macroinvertebrate assemblage composition; macroinvertebrate drift timing and density; and through niche utilisation determined from stable isotope data. Patterns in the macroinvertebrate assemblages of the headwaters of the Keiskamma River appear to be driven more strongly by flow rate and seasonal influences, but fish presence and biotope availability were also significant drivers. Niche shifts due to predator presence were not easy to detect and, while patterns of influence may have been evident, they were not found to be significant. However, I demonstrated that salmonids selectively feed on native fish species when the opportunity is presented, occupying significantly higher trophic levels when co-occurring with native fish than in invaded reaches where native fish are absent. Drift timing and density were demonstrated to be significantly different between reach for specific macroinvertebrate species from the Ephemeroptera and Plecoptera, under differing fish predation regimes, in agreement with what has been observed from studies in rivers elsewhere. In freshwater ecosystems of South Africa and worldwide, mitigation of negative effects of alien fishes through their removal using piscicides may also affect non-target organisms. To better understand the effects of just such a removal operation, employed for the first time in the history of freshwater conservation in South Africa, macroinvertebrate communities were assessed for non-target effects of rotenone. The fish eradication operations were demonstrated to have a short-term negative effect on the macroinvertebrate assemblage, through water quality index measurements and alteration of densities of macroinvertebrate taxa collected from stone surfaces. However, no long-term detrimental impact was observed as macroinvertebrate faunas returned to a comparable pre-treatment state within a year of each rotenone application.
- Full Text:
The evaluation of potential dietary media, measurement parameters and storage techniques for use in forensic entomotoxicology
- Mbatha, Erica Isabel Tavares Da Silva
- Authors: Mbatha, Erica Isabel Tavares Da Silva
- Date: 2018
- Subjects: Blowflies -- Feeding and feeds , Blowflies -- Larvae , Blowflies -- Physiology , Blowflies -- Collection and preservation , Poisons -- Analysis , Death -- Causes , Forensic pathology , Forensic entomology , Forensic entomotoxicology
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/63323 , vital:28393
- Description: The term forensic entomotoxicology was coined by Pounder and is used to describe the process of using insects to determine the presence or absence of toxicants in decomposing corpses. Forensic entomotoxicology is most applicable when the orthodox sources of evidence (i.e. blood and urine) are no longer available for testing due to the degree of putrefaction as a result of the decomposition process. As the field is relatively new, various authors have conducted studies to determine the effects of different toxicants on different insects. These studies have all been conducted in the absence of a standardised protocol and we hypothesise that this has led to conflicting results (i.e. two different authors will conduct a study using the same toxicant and model insect and the effects on the insects will differ significantly). The aim of this thesis was to identify the areas which might have led to the artefacts in the results and identify ways in which to standardise them. The three areas selected were the feeding substrates and the measures taken to quantify growth rate, as well as the preservation techniques that should be used for preserving larval flies. The recommendation from the literature review was that artificial diets would be the most appropriate dietary media to use for entomotoxicological studies. An artificial diet was selected and modified for potential used in entomotoxicological studies. Four different diets (no meat treatment, fish, beef and pork artificial diets) were used to rear Chrysomya chloropyga larvae and their growth rates were measured using length and width. The fly larvae reared on the fish and no meat treatment diets did not reach pupation stage. The beef and pork diets produced the largest larvae and the flies in these treatments reached adult stage. The recommendation was that the beef and pork treatments be tested with various toxicants to establish their stability in the matrix and the diet that provides the toxicants with the most stability should be used for future entomotoxicological studies. The two other factors selected for standardisation were the parameters used to quantify growth rate, as well as the preservation techniques used to store empty Chrysomya chloropyga pupal casings and Calliphora croceipalpis third instar larvae. Previous authors have suggested that width be used as an alternative to length to quantify growth rate. The results from this thesis show that length should continue to be used as the standard parameter because the incremental change in length is much larger than the change in width, and these larger increments allow for greater resolution when estimating the age of the larvae. Various authors have also suggested that pupal casings should be stored without any preservative, whereas fly larvae should be stored in concentrations of ethanol >70%. The results in this thesis have shown that the concentration of ethanol does not make any significant difference to the proportional change of length and width of the empty pupal casings and the third instar larvae. The recommendation is that when selecting the preservation technique, the integrity of the specimen for examination of other evidence (i.e. DNA or toxicological extraction) should take precedence. Although this thesis has not completely standardised the protocol for forensic entomotoxicology, it has indicated the areas that need to be focused on in order for standardisation to occur. Future studies should focus on standardisation, as this makes studies more comparable and ultimately makes entomotoxicological evidence admissible in the court of law.
- Full Text:
- Authors: Mbatha, Erica Isabel Tavares Da Silva
- Date: 2018
- Subjects: Blowflies -- Feeding and feeds , Blowflies -- Larvae , Blowflies -- Physiology , Blowflies -- Collection and preservation , Poisons -- Analysis , Death -- Causes , Forensic pathology , Forensic entomology , Forensic entomotoxicology
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/63323 , vital:28393
- Description: The term forensic entomotoxicology was coined by Pounder and is used to describe the process of using insects to determine the presence or absence of toxicants in decomposing corpses. Forensic entomotoxicology is most applicable when the orthodox sources of evidence (i.e. blood and urine) are no longer available for testing due to the degree of putrefaction as a result of the decomposition process. As the field is relatively new, various authors have conducted studies to determine the effects of different toxicants on different insects. These studies have all been conducted in the absence of a standardised protocol and we hypothesise that this has led to conflicting results (i.e. two different authors will conduct a study using the same toxicant and model insect and the effects on the insects will differ significantly). The aim of this thesis was to identify the areas which might have led to the artefacts in the results and identify ways in which to standardise them. The three areas selected were the feeding substrates and the measures taken to quantify growth rate, as well as the preservation techniques that should be used for preserving larval flies. The recommendation from the literature review was that artificial diets would be the most appropriate dietary media to use for entomotoxicological studies. An artificial diet was selected and modified for potential used in entomotoxicological studies. Four different diets (no meat treatment, fish, beef and pork artificial diets) were used to rear Chrysomya chloropyga larvae and their growth rates were measured using length and width. The fly larvae reared on the fish and no meat treatment diets did not reach pupation stage. The beef and pork diets produced the largest larvae and the flies in these treatments reached adult stage. The recommendation was that the beef and pork treatments be tested with various toxicants to establish their stability in the matrix and the diet that provides the toxicants with the most stability should be used for future entomotoxicological studies. The two other factors selected for standardisation were the parameters used to quantify growth rate, as well as the preservation techniques used to store empty Chrysomya chloropyga pupal casings and Calliphora croceipalpis third instar larvae. Previous authors have suggested that width be used as an alternative to length to quantify growth rate. The results from this thesis show that length should continue to be used as the standard parameter because the incremental change in length is much larger than the change in width, and these larger increments allow for greater resolution when estimating the age of the larvae. Various authors have also suggested that pupal casings should be stored without any preservative, whereas fly larvae should be stored in concentrations of ethanol >70%. The results in this thesis have shown that the concentration of ethanol does not make any significant difference to the proportional change of length and width of the empty pupal casings and the third instar larvae. The recommendation is that when selecting the preservation technique, the integrity of the specimen for examination of other evidence (i.e. DNA or toxicological extraction) should take precedence. Although this thesis has not completely standardised the protocol for forensic entomotoxicology, it has indicated the areas that need to be focused on in order for standardisation to occur. Future studies should focus on standardisation, as this makes studies more comparable and ultimately makes entomotoxicological evidence admissible in the court of law.
- Full Text:
The epidemiology of African horse sickness in the Eastern Cape, South Africa
- Authors: Riddin, Megan Amy
- Date: 2017
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/64460 , vital:28546
- Description: Expected release date-May 2019
- Full Text:
- Authors: Riddin, Megan Amy
- Date: 2017
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/64460 , vital:28546
- Description: Expected release date-May 2019
- Full Text:
Aquatic–terrestrial trophic linkages via riverine invertebrates in a South African catchment
- Authors: Moyo, Sydney
- Date: 2016
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/54427 , vital:26564
- Description: Rivers play a vital role in human livelihoods and are likely to undergo substantial alteration due to climate and land use changes from an increasing human population. Mitigating the pressures facing rivers in the world requires scientists and environmental managers to understand the ecological mechanisms, and ultimately the strength, of connections between ecosystems. This understanding of connections between adjacent habitats will enable environmental managers to predict the consequences of perturbing these linkages in the future. In this thesis, aquatic-terrestrial linkages in rivers were investigated using ecologically meaningful variables including abundances, biomasses, stable isotopes and fatty acids. This study is part of a larger project entitled “Connectivity through allochthony: reciprocal links between adjacent aquatic and terrestrial ecosystems in South Africa”, in which a team of researchers assessed a variety of pathways connecting riverine and estuarine systems to land within a catchment in the Eastern Cape, South Africa. I conceptualised the flow of energy within a temperate southern hemisphere river (the Kowie River) within theoretical models of energy flow such as the River Continuum Concept (RCC; presents lotic systems as being longitudinally linked with food webs in shaded headwaters being principally driven by allochthonous energy, with the addition of autochthonous food as a minor carbon source in the lower reaches) and the Riverine Productivity Model (RPM; proposes consumers derive most of their energy from local production of phytoplankton, benthic algae and aquatic plants, as well as directly from riparian zones via terrestrial leaf litter). Using the RCC as a starting point, I collected macroinvertebrates (September 2012 to May 2013) along a longitudinal gradient and grouped them into functional feeding groups (FFGs). The results revealed that gatherers and filterers dominated in the Kowie River, and together represented 50 – 83% of the invertebrate assemblages. There was a general paucity of shredders (relative abundance was ≤ 10% across all sites and seasons). The changes in relative abundances of different FFGs did not follow predictions of the RCC along the longitudinal gradient, as there were no correlations of community structure with some physical attributes (stream width, canopy cover, distance of river) that changed along the river continuum. However, FFG abundances were related to water velocity, total dissolved solids and canopy cover. Broadly, the Kowie River data showed that changes in relative abundances of FFGs along the river continuum could not be explained by changes in physical attributes alone, and may be highly influenced by the availability of food and the chemistry of the stream. Analysis of stable carbon (δ13C) and nitrogen (δ15N) isotopes was used to estimate the contributions of algal and land-based production to consumers over space (six sites) and time (November 2012 to September 2013). Carbon contributions determined by the use of mixing models (Stable Isotope Analysis in R) revealed that consumers in the headwater assimilated mainly terrestrially-derived organic matter, with consumers in the middle and lower reaches assimilating autochthonous basal resources (macrophytes and algae). The findings from this river supported aspects of the RCC (at the headwaters; terrestrial organic matter made up 41% of consumer diets), but overall the data supported the predictions of the RPM (local production made the highest contributions of 50 – 86% to all FFGs across all seasons). The carbon isotopes of consumers and their food sources changed substantially every season, indicating that samples of food sources and consumers should be analysed many times throughout the year to capture that variability and to ensure that ephemeral components of the food web are not missed. To validate the findings from the isotope data, fatty acids were used as complementary tracers to determine the contributions of algal versus terrestrial organic matter to the consumers. Fatty acid tracers for terrestrial (Ʃω3/Ʃω6; 18:2ω6; 18:3ω3) vs aquatic (Ʃω3/Ʃω6; 20:5ω3) sources corroborated the findings from the isotope data set, as the mean ratio of Ʃω3/Ʃω6 in consumers was less than one at the headwaters (indicating allochthony), while middle and lower reaches were associated with Ʃω3/Ʃω6 > 1 (indicating autochthony). In addition to the tracer and FFG analyses for examining trophic connections between land and river, the bidirectional exchange of organisms between the riparian zone and the river was assessed using floating pyramidal traps (to measure emergence) and pan traps (for infalling invertebrates) placed at different sites in the river and the biomass in each trap was determined. The exchanges were variable over space and time, with emergence peaking in summer (169 to 1402 mg m-2 day-1) and declining in winter (3 to 28 mg m-2 day-1). Similarly, infalling invertebrates increased in summer (413 to 679 mg m-2 day-1) and declined in winter (11 to 220 mg m-2 day-1). Biomass measurements are indications of quantity that ignore nutritional quality, so I determined the bidirectional flow of invertebrates using absolute concentrations of physiologically important biochemical compounds (essential and polyunsaturated fatty acids). The fluxes of emergent and infalling arthropods peaked in summer (emergence = 0.3 to 18 mg m-2 day-1 and terrestrial infall = 0.3 to 3 mg m-2 day-1) and declined in winter (emergence = 0.01 to 0.51 mg m-2 day-1 and terrestrial infall = 0.01 to 0.03 mg m-2 day-1). However, during some seasons, no significant differences in polyunsaturated fatty acid flux in either direction were observed; this finding indicated the balance of reciprocal subsidisation via reciprocal flows of animals. Factors such as air temperature and algal productivity affected the reciprocal flows between adjacent habitats, with algal productivity being positively related to emergence while air temperature was positively correlated to infalling terrestrial invertebrates. This research enhances the growing body of literature on the function of riverine systems and offers some invaluable information on the flow of energy and the role played by invertebrates in translocating nutrients from terrestrial systems to aquatic systems and vice versa. This study unifies the concepts of the RCC and RPM and shows that these concepts are not limited only to large rivers, but are applicable to small southern temperate rivers too. However, some tenets of the theoretical models were challenged. For example, it challenges the proposition by the RCC that the fine particulate organic matter leaked from upstream breakdown of coarse particulate organic matter is predominantly allochthonous. Additionally, this study showed that in the headwaters, the RPM underestimated the role of autochthony. Overall, the results showed that the Kowie River and its riparian area are intrinsically connected. Once we understand the mechanisms controlling connections and subsidies across ecotones, we can then start to predict the consequences of disruptions to these connections by climate change and/or land use changes. To make predictions about future perturbations to rivers and riparian zones, studies like this, which considers the form and magnitude of subsidies, are needed to provide baseline information. Algal resources (e.g. epiphyton), macrophytes, riparian plants, terrestrial organisms and aquatic organisms all contributed to aquatic and terrestrial linkages in the Kowie River; therefore, it is important to conserve the different components of these ecosystems.
- Full Text:
- Authors: Moyo, Sydney
- Date: 2016
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/54427 , vital:26564
- Description: Rivers play a vital role in human livelihoods and are likely to undergo substantial alteration due to climate and land use changes from an increasing human population. Mitigating the pressures facing rivers in the world requires scientists and environmental managers to understand the ecological mechanisms, and ultimately the strength, of connections between ecosystems. This understanding of connections between adjacent habitats will enable environmental managers to predict the consequences of perturbing these linkages in the future. In this thesis, aquatic-terrestrial linkages in rivers were investigated using ecologically meaningful variables including abundances, biomasses, stable isotopes and fatty acids. This study is part of a larger project entitled “Connectivity through allochthony: reciprocal links between adjacent aquatic and terrestrial ecosystems in South Africa”, in which a team of researchers assessed a variety of pathways connecting riverine and estuarine systems to land within a catchment in the Eastern Cape, South Africa. I conceptualised the flow of energy within a temperate southern hemisphere river (the Kowie River) within theoretical models of energy flow such as the River Continuum Concept (RCC; presents lotic systems as being longitudinally linked with food webs in shaded headwaters being principally driven by allochthonous energy, with the addition of autochthonous food as a minor carbon source in the lower reaches) and the Riverine Productivity Model (RPM; proposes consumers derive most of their energy from local production of phytoplankton, benthic algae and aquatic plants, as well as directly from riparian zones via terrestrial leaf litter). Using the RCC as a starting point, I collected macroinvertebrates (September 2012 to May 2013) along a longitudinal gradient and grouped them into functional feeding groups (FFGs). The results revealed that gatherers and filterers dominated in the Kowie River, and together represented 50 – 83% of the invertebrate assemblages. There was a general paucity of shredders (relative abundance was ≤ 10% across all sites and seasons). The changes in relative abundances of different FFGs did not follow predictions of the RCC along the longitudinal gradient, as there were no correlations of community structure with some physical attributes (stream width, canopy cover, distance of river) that changed along the river continuum. However, FFG abundances were related to water velocity, total dissolved solids and canopy cover. Broadly, the Kowie River data showed that changes in relative abundances of FFGs along the river continuum could not be explained by changes in physical attributes alone, and may be highly influenced by the availability of food and the chemistry of the stream. Analysis of stable carbon (δ13C) and nitrogen (δ15N) isotopes was used to estimate the contributions of algal and land-based production to consumers over space (six sites) and time (November 2012 to September 2013). Carbon contributions determined by the use of mixing models (Stable Isotope Analysis in R) revealed that consumers in the headwater assimilated mainly terrestrially-derived organic matter, with consumers in the middle and lower reaches assimilating autochthonous basal resources (macrophytes and algae). The findings from this river supported aspects of the RCC (at the headwaters; terrestrial organic matter made up 41% of consumer diets), but overall the data supported the predictions of the RPM (local production made the highest contributions of 50 – 86% to all FFGs across all seasons). The carbon isotopes of consumers and their food sources changed substantially every season, indicating that samples of food sources and consumers should be analysed many times throughout the year to capture that variability and to ensure that ephemeral components of the food web are not missed. To validate the findings from the isotope data, fatty acids were used as complementary tracers to determine the contributions of algal versus terrestrial organic matter to the consumers. Fatty acid tracers for terrestrial (Ʃω3/Ʃω6; 18:2ω6; 18:3ω3) vs aquatic (Ʃω3/Ʃω6; 20:5ω3) sources corroborated the findings from the isotope data set, as the mean ratio of Ʃω3/Ʃω6 in consumers was less than one at the headwaters (indicating allochthony), while middle and lower reaches were associated with Ʃω3/Ʃω6 > 1 (indicating autochthony). In addition to the tracer and FFG analyses for examining trophic connections between land and river, the bidirectional exchange of organisms between the riparian zone and the river was assessed using floating pyramidal traps (to measure emergence) and pan traps (for infalling invertebrates) placed at different sites in the river and the biomass in each trap was determined. The exchanges were variable over space and time, with emergence peaking in summer (169 to 1402 mg m-2 day-1) and declining in winter (3 to 28 mg m-2 day-1). Similarly, infalling invertebrates increased in summer (413 to 679 mg m-2 day-1) and declined in winter (11 to 220 mg m-2 day-1). Biomass measurements are indications of quantity that ignore nutritional quality, so I determined the bidirectional flow of invertebrates using absolute concentrations of physiologically important biochemical compounds (essential and polyunsaturated fatty acids). The fluxes of emergent and infalling arthropods peaked in summer (emergence = 0.3 to 18 mg m-2 day-1 and terrestrial infall = 0.3 to 3 mg m-2 day-1) and declined in winter (emergence = 0.01 to 0.51 mg m-2 day-1 and terrestrial infall = 0.01 to 0.03 mg m-2 day-1). However, during some seasons, no significant differences in polyunsaturated fatty acid flux in either direction were observed; this finding indicated the balance of reciprocal subsidisation via reciprocal flows of animals. Factors such as air temperature and algal productivity affected the reciprocal flows between adjacent habitats, with algal productivity being positively related to emergence while air temperature was positively correlated to infalling terrestrial invertebrates. This research enhances the growing body of literature on the function of riverine systems and offers some invaluable information on the flow of energy and the role played by invertebrates in translocating nutrients from terrestrial systems to aquatic systems and vice versa. This study unifies the concepts of the RCC and RPM and shows that these concepts are not limited only to large rivers, but are applicable to small southern temperate rivers too. However, some tenets of the theoretical models were challenged. For example, it challenges the proposition by the RCC that the fine particulate organic matter leaked from upstream breakdown of coarse particulate organic matter is predominantly allochthonous. Additionally, this study showed that in the headwaters, the RPM underestimated the role of autochthony. Overall, the results showed that the Kowie River and its riparian area are intrinsically connected. Once we understand the mechanisms controlling connections and subsidies across ecotones, we can then start to predict the consequences of disruptions to these connections by climate change and/or land use changes. To make predictions about future perturbations to rivers and riparian zones, studies like this, which considers the form and magnitude of subsidies, are needed to provide baseline information. Algal resources (e.g. epiphyton), macrophytes, riparian plants, terrestrial organisms and aquatic organisms all contributed to aquatic and terrestrial linkages in the Kowie River; therefore, it is important to conserve the different components of these ecosystems.
- Full Text:
Predators of aerial insects and riparian cross-boundary trophic dynamics: web-building spiders, dragonflies and damselflies
- Authors: Chari, Lenin Dzibakwe
- Date: 2016
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/55791 , vital:26734
- Description: This thesis characterises the cross-boundary trophic interactions of a relatively small model ecosystem, the Kowie River (Eastern Cape of South Africa), to explore their epistemic implications for systems ecology. Using web-building spiders and odonates (dragonflies and damselflies) as model organisms, I sought to investigate whether the diets of predators of aerial insects could be used to assess the strength of the trophic connectivity between freshwater and terrestrial systems in relation to variables such as stream width, distance from the river and aquatic insect emergence rates and abundances. Predator diet composition was determined by using a combination of diet analysis tools: direct observations of cross-subsidies, naturally-abundant stable (carbon and nitrogen) isotope analysis and fatty acid analysis. I also sought to reveal feeding niches and guilds among riparian aerial predators and investigate how the environment influenced predators’ access to aquatic prey subsidies. As emergent aquatic insect abundances decreased with an increase in distance from the river, and increased with stream width and seasonal changes from winter to summer, stable isotope and fatty acid analyses revealed distinct changes in web-building spider diet composition. Examination of the fatty acid eicosapentaenoic acid, a component commonly used as an indicator of consumer reliance on aquatic nutritional subsidies, showed that aquatic subsidies extended further inland at the wider sections of the river. Spiders and odonates at the wider sections of the Kowie River generally received more subsidies (56 – 70%) than those at the narrower sections (25 – 60%). When terrestrial insect biomass was distinctly low in winter, the benefit of aquatic subsidisation to spiders was relatively lower at the narrower sections of the Kowie River relative to the wide sections. As such, riparian areas adjacent to wide parts of the river were more likely to support larger populations of aerial predators than those at the narrow sections. Apart from the diet changes across time and space, there was evidence of inter-specific niche partitioning in both spiders and odonates, but no differences were observed between males and females of the same species. Results showed odonates of different sizes and hunting strategies had separate dietary niches, hence varied access to aquatic nutritional subsidies. The larger odonate taxa that frequently foraged mid-air had more varied diets and relied less on aquatic emergent insects than the smaller odonates that foraged from perches near the river. There was also evidence of niche partitioning amongst the spiders, as those that built horizontal webs captured more aquatic insects (40 – 78%) than the vertical orb-web builders (20 – 66%). This study showed that the nature and extent of trophic cross-boundary linkages in riparian areas largely depended on the availability of subsidies that varied seasonally and spatially. The width of the stream and seasonal variability emerged as important predictors of emergent insect abundances/biomasses that influenced predator feeding niches. The high mobility of odonates made their reliance on aquatic nutritional subsidies different from the less mobile spiders. The link between the width of the river and the extent of trophic connectivity has implications for riparian area management and definition of riparian buffer zones. However, the variation in diet niches amongst terrestrial consumers makes the results area-specific, and more studies are required that incorporate additional terrestrial predators in other fluvial systems so that we can make some generalizations on the dynamics of riparian trophic cross-boundary links.
- Full Text:
- Authors: Chari, Lenin Dzibakwe
- Date: 2016
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/55791 , vital:26734
- Description: This thesis characterises the cross-boundary trophic interactions of a relatively small model ecosystem, the Kowie River (Eastern Cape of South Africa), to explore their epistemic implications for systems ecology. Using web-building spiders and odonates (dragonflies and damselflies) as model organisms, I sought to investigate whether the diets of predators of aerial insects could be used to assess the strength of the trophic connectivity between freshwater and terrestrial systems in relation to variables such as stream width, distance from the river and aquatic insect emergence rates and abundances. Predator diet composition was determined by using a combination of diet analysis tools: direct observations of cross-subsidies, naturally-abundant stable (carbon and nitrogen) isotope analysis and fatty acid analysis. I also sought to reveal feeding niches and guilds among riparian aerial predators and investigate how the environment influenced predators’ access to aquatic prey subsidies. As emergent aquatic insect abundances decreased with an increase in distance from the river, and increased with stream width and seasonal changes from winter to summer, stable isotope and fatty acid analyses revealed distinct changes in web-building spider diet composition. Examination of the fatty acid eicosapentaenoic acid, a component commonly used as an indicator of consumer reliance on aquatic nutritional subsidies, showed that aquatic subsidies extended further inland at the wider sections of the river. Spiders and odonates at the wider sections of the Kowie River generally received more subsidies (56 – 70%) than those at the narrower sections (25 – 60%). When terrestrial insect biomass was distinctly low in winter, the benefit of aquatic subsidisation to spiders was relatively lower at the narrower sections of the Kowie River relative to the wide sections. As such, riparian areas adjacent to wide parts of the river were more likely to support larger populations of aerial predators than those at the narrow sections. Apart from the diet changes across time and space, there was evidence of inter-specific niche partitioning in both spiders and odonates, but no differences were observed between males and females of the same species. Results showed odonates of different sizes and hunting strategies had separate dietary niches, hence varied access to aquatic nutritional subsidies. The larger odonate taxa that frequently foraged mid-air had more varied diets and relied less on aquatic emergent insects than the smaller odonates that foraged from perches near the river. There was also evidence of niche partitioning amongst the spiders, as those that built horizontal webs captured more aquatic insects (40 – 78%) than the vertical orb-web builders (20 – 66%). This study showed that the nature and extent of trophic cross-boundary linkages in riparian areas largely depended on the availability of subsidies that varied seasonally and spatially. The width of the stream and seasonal variability emerged as important predictors of emergent insect abundances/biomasses that influenced predator feeding niches. The high mobility of odonates made their reliance on aquatic nutritional subsidies different from the less mobile spiders. The link between the width of the river and the extent of trophic connectivity has implications for riparian area management and definition of riparian buffer zones. However, the variation in diet niches amongst terrestrial consumers makes the results area-specific, and more studies are required that incorporate additional terrestrial predators in other fluvial systems so that we can make some generalizations on the dynamics of riparian trophic cross-boundary links.
- Full Text:
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