Phylogeography and reproductive isolation of the brown mussel, Perna perna, on the South African coastline
- Authors: Barker, Cassandra
- Date: 2021-10
- Subjects: Mexilhao mussel South Africa , Phylogeography South Africa , Mexilhao mussel Reproduction , Mexilhao mussel Genetics , Intertidal organisms South Africa , Mexilhao mussel Climatic factors South Africa , Cytochrome oxidase , Mitochondrial DNA , Haplotype network
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190041 , vital:44958
- Description: Maintenance of a species’ abundance and adaptive potential is partially dependent on its genetic diversity. Distinct genetic lineages within a species can differ significantly in their resistance and resilience to environmental pressure. An assessment of such differences is key to grasp the adaptive potential of a species. Physical environmental conditions are significant determinants of the distribution of species and their genetic lineages. Under climate change scenarios, the assessment of the temporal stability of the spatial distribution of genetic structure has important consequences for conservation as it offers key insights into the adaptive potential and evolutionary capacity of a species. This thesis investigated the phylogeography and reproductive isolation of Perna perna, the brown mussel. This species is an ecologically and economically important intertidal mussel on South Africa’s coastline. It was determined in 2007 that there were two distinct genetic lineages of P. perna present on South Africa’s coastline, the Eastern and Western lineage. This thesis compared mitochondrial DNA between samples collected in 2007 and samples collected in 2019 to determine differences in genetic structure of P. perna over time. This thesis further investigated the reproductive timing of the two lineages to determine if this contributes to the maintenance of the genetic structure of this species. The results show that there is a shift in the distribution of the Eastern lineage since 2007, an increase in the range of the overlap region, and there was a change in genetic diversity in the form of private haplotypes between 2007 and 2019. The lineages have unsynchronised spawning and reproductive patterns are more closely linked to changes in temperature. While the lineages have shown slight distributional changes over time, the influence of environmental conditions and the predicted changes in sea surface temperatures could see a change in future populations’ fitness and dispersal. Thus, changes in sea surface temperature could affect reproductive timing and the future genetic stability of the species. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2021
- Full Text:
- Date Issued: 2021-10
- Authors: Barker, Cassandra
- Date: 2021-10
- Subjects: Mexilhao mussel South Africa , Phylogeography South Africa , Mexilhao mussel Reproduction , Mexilhao mussel Genetics , Intertidal organisms South Africa , Mexilhao mussel Climatic factors South Africa , Cytochrome oxidase , Mitochondrial DNA , Haplotype network
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/190041 , vital:44958
- Description: Maintenance of a species’ abundance and adaptive potential is partially dependent on its genetic diversity. Distinct genetic lineages within a species can differ significantly in their resistance and resilience to environmental pressure. An assessment of such differences is key to grasp the adaptive potential of a species. Physical environmental conditions are significant determinants of the distribution of species and their genetic lineages. Under climate change scenarios, the assessment of the temporal stability of the spatial distribution of genetic structure has important consequences for conservation as it offers key insights into the adaptive potential and evolutionary capacity of a species. This thesis investigated the phylogeography and reproductive isolation of Perna perna, the brown mussel. This species is an ecologically and economically important intertidal mussel on South Africa’s coastline. It was determined in 2007 that there were two distinct genetic lineages of P. perna present on South Africa’s coastline, the Eastern and Western lineage. This thesis compared mitochondrial DNA between samples collected in 2007 and samples collected in 2019 to determine differences in genetic structure of P. perna over time. This thesis further investigated the reproductive timing of the two lineages to determine if this contributes to the maintenance of the genetic structure of this species. The results show that there is a shift in the distribution of the Eastern lineage since 2007, an increase in the range of the overlap region, and there was a change in genetic diversity in the form of private haplotypes between 2007 and 2019. The lineages have unsynchronised spawning and reproductive patterns are more closely linked to changes in temperature. While the lineages have shown slight distributional changes over time, the influence of environmental conditions and the predicted changes in sea surface temperatures could see a change in future populations’ fitness and dispersal. Thus, changes in sea surface temperature could affect reproductive timing and the future genetic stability of the species. , Thesis (MSc) -- Faculty of Science, Zoology and Entomology, 2021
- Full Text:
- Date Issued: 2021-10
Biogeographic patterns of endolithic cyanobacteria and their negative impacts on mussels along the South African coast
- Authors: Ndhlovu, Aldwin
- Date: 2020
- Subjects: Cyanobacteria -- South Africa , Cyanobacteria -- Geographical distribution , Prokaryotes -- South Africa , Mexilhao mussel -- South Africa , Mytilus galloprovincialis -- South Africa , Coastal biology -- South Africa , Coastal ecology -- South Africa , Mussels -- Geographical distribution , Mussels -- Predators of , Mussels -- Mortality -- South Africa , Mussels -- Ecology -- South Africa , Mussels -- Growth -- South Africa , Mussels -- Fertility -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/144656 , vital:38367
- Description: Endolithic cyanobacterial species occur in a wide range of environments including cold and hot deserts as well as marine systems where they attack biological material such as corals and the shells of molluscs including limpets, mussels and abalone. Endoliths live as parasites in mussel shells, where they erode and extract calcium carbonate leading to shell weakening, creating fracture holes that lead to shell collapse and death, but they also have positive effects when they lead to discolouration of mussel shells hence giving them the ability to reduce stressful heat gain during periods of extreme heat stress. Mussels are ecological engineers on which the abundance and diversity of associated species assemblages depend. Understanding how endolithic cyanobacteria affect mussels will not only help in predicting future patterns of mussel abundances, but also future patterns of the infauna that depend on them. Firstly, I identified endolithic species infesting mussels and assessed the prevalence of endolithic parasitism in two intertidal mussel species in South Africa, the native Perna perna and the invasive Mytilus galloprovincialis. Large-scale surveys of endolithic infestation of mussels were conducted along 2500 km of the South African coast, covering three biogeographic regions: the subtropical east coast, dominated by P. perna, the warm temperate south coast where the indigenous species coexists with M. galloprovincialis, and the cool temperate west coast which is dominated by M. galloprovincialis. The prevalence of endolithic infestation was higher in the cool temperate bioregion than in the warm temperate and subtropical bioregions which did not differ and for P. perna endolithic species assemblages revealed clear groupings by bioregion. Results for endolithic induced mortality followed the same trend, with no significant difference between the two mussel species where they coexist and these results attribute biogeography of endoliths to environmental factors rather than host identity. Secondly, I assessed energy budgets of infested and clean mussels, to evaluate the energetic cost of infestation. This involved measuring energy acquisition, expenditure, calculating scope for growth and lethal temperatures (LT50s). The results revealed that endolithic cyanobacteria have a negative effect on scope for growth due to increased metabolic rates for infested mussels, with no effect of endoliths on the rates or efficiency of energy acquisition through filtration and no effect on lethal temperatures. The effects of infestation were then examined in more detail through a qualitative and quantitative analysis of mussel gonads and byssal attachment strength to the substratum. Endolithic infestation was found to affect reproduction by affecting the size (mass) of gonads, but not the density of eggs within them. Attachment strength was affected by endolithic infestation with very infested mussels requiring much less force to detach them from the substratum compared to mussels with low or no infestation. These results show that endolithic infestation affects mussel fitness by directly affecting attachment strength and by reducing their reproductive output. Thirdly, endolithic succession within mussel shells was examined by assessing endolithic species composition in different regions of the shell and as a function of time. The results on the spatial distribution of endolith species within a shell supported those for temporal succession in shells deployed in the field. Endolithic species that were early colonists of clean shells were similar to those that were found in the distal edge, the new and growing region of the shell and species that arrived late in succession were similar to endolithic species found near the umbo, the oldest region of the shell. Overall, the study shows that endolithic cyanobacteria show the effects of biogeography on species distribution and clear patterns of succession within mussel shells. Cyanobacteria affect mussels negatively; they lead to low scope for growth and hence low growth rates, low reproductive output and reduced attachment strength for infested mussels. This, in turn is expected to have indirect consequences for other species that rely on mussels as ecological engineers for their survival.
- Full Text:
- Date Issued: 2020
- Authors: Ndhlovu, Aldwin
- Date: 2020
- Subjects: Cyanobacteria -- South Africa , Cyanobacteria -- Geographical distribution , Prokaryotes -- South Africa , Mexilhao mussel -- South Africa , Mytilus galloprovincialis -- South Africa , Coastal biology -- South Africa , Coastal ecology -- South Africa , Mussels -- Geographical distribution , Mussels -- Predators of , Mussels -- Mortality -- South Africa , Mussels -- Ecology -- South Africa , Mussels -- Growth -- South Africa , Mussels -- Fertility -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/144656 , vital:38367
- Description: Endolithic cyanobacterial species occur in a wide range of environments including cold and hot deserts as well as marine systems where they attack biological material such as corals and the shells of molluscs including limpets, mussels and abalone. Endoliths live as parasites in mussel shells, where they erode and extract calcium carbonate leading to shell weakening, creating fracture holes that lead to shell collapse and death, but they also have positive effects when they lead to discolouration of mussel shells hence giving them the ability to reduce stressful heat gain during periods of extreme heat stress. Mussels are ecological engineers on which the abundance and diversity of associated species assemblages depend. Understanding how endolithic cyanobacteria affect mussels will not only help in predicting future patterns of mussel abundances, but also future patterns of the infauna that depend on them. Firstly, I identified endolithic species infesting mussels and assessed the prevalence of endolithic parasitism in two intertidal mussel species in South Africa, the native Perna perna and the invasive Mytilus galloprovincialis. Large-scale surveys of endolithic infestation of mussels were conducted along 2500 km of the South African coast, covering three biogeographic regions: the subtropical east coast, dominated by P. perna, the warm temperate south coast where the indigenous species coexists with M. galloprovincialis, and the cool temperate west coast which is dominated by M. galloprovincialis. The prevalence of endolithic infestation was higher in the cool temperate bioregion than in the warm temperate and subtropical bioregions which did not differ and for P. perna endolithic species assemblages revealed clear groupings by bioregion. Results for endolithic induced mortality followed the same trend, with no significant difference between the two mussel species where they coexist and these results attribute biogeography of endoliths to environmental factors rather than host identity. Secondly, I assessed energy budgets of infested and clean mussels, to evaluate the energetic cost of infestation. This involved measuring energy acquisition, expenditure, calculating scope for growth and lethal temperatures (LT50s). The results revealed that endolithic cyanobacteria have a negative effect on scope for growth due to increased metabolic rates for infested mussels, with no effect of endoliths on the rates or efficiency of energy acquisition through filtration and no effect on lethal temperatures. The effects of infestation were then examined in more detail through a qualitative and quantitative analysis of mussel gonads and byssal attachment strength to the substratum. Endolithic infestation was found to affect reproduction by affecting the size (mass) of gonads, but not the density of eggs within them. Attachment strength was affected by endolithic infestation with very infested mussels requiring much less force to detach them from the substratum compared to mussels with low or no infestation. These results show that endolithic infestation affects mussel fitness by directly affecting attachment strength and by reducing their reproductive output. Thirdly, endolithic succession within mussel shells was examined by assessing endolithic species composition in different regions of the shell and as a function of time. The results on the spatial distribution of endolith species within a shell supported those for temporal succession in shells deployed in the field. Endolithic species that were early colonists of clean shells were similar to those that were found in the distal edge, the new and growing region of the shell and species that arrived late in succession were similar to endolithic species found near the umbo, the oldest region of the shell. Overall, the study shows that endolithic cyanobacteria show the effects of biogeography on species distribution and clear patterns of succession within mussel shells. Cyanobacteria affect mussels negatively; they lead to low scope for growth and hence low growth rates, low reproductive output and reduced attachment strength for infested mussels. This, in turn is expected to have indirect consequences for other species that rely on mussels as ecological engineers for their survival.
- Full Text:
- Date Issued: 2020
The role of upwelling in determining the composition, species distribution and genetic structure of intertidal communities in a time of climate change
- Lourenço, Carla Sofia Emídio Rodrigues
- Authors: Lourenço, Carla Sofia Emídio Rodrigues
- Date: 2018
- Subjects: Upwelling (Oceanography) , Intertidal organisms -- Morocco -- Atlantic Coast , Intertidal organisms -- Canary Current -- Effect of water currents on , Intertidal animals -- Canary Current -- Effect of water currents on , Intertidal animals -- Morocco -- Atlantic Coas , Mytilus galloprovincialis -- Morocco -- Atlantic Coast , Mytilus galloprovincialis -- Canary Current -- Effect of water currents on , Intertidal ecology -- Canary Current , Sea surface microlayer -- Morocco -- Atlantic Coast
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/61614 , vital:28042
- Description: Upwelling is an oceanographic process that strongly influences coastal species and the communities they belong to. In upwelling areas, colder, denser, nutrient-rich subsurface waters are transported to the nearshore surface, replacing warmer superficial waters that are advected offshore. Such effects influence the composition and dynamics of coastal communities, for example by affecting species abundance, recruitment, dispersal and distribution. Upwelling areas are key model regions to study the responses of coastal species to climate change because they are characterized by cooler conditions and experience lower warming rates than adjacent regions. In particular, intertidal rocky shore species are ideal coastal sentinel organisms to study distributional changes driven by climate warming because they inhabit the interface between marine and terrestrial habitats and are exposed to extremely severe environmental conditions. In fact, sharp distributional shifts have been reported for multiple intertidal species as a response to ocean warming. Although some studies have investigated the role of upwelling in influencing abundance and distribution of intertidal species, little is known about its potential as refugia against climate warming and the degree to which upwelling shapes species genetic structure is yet not fully understood. The aim of this thesis is to understand the influence of the Canary Current upwelling system on intertidal community composition, including species distribution and the genetic structure of intertidal species under current climate change. To do this, I investigated community structure of intertidal assemblages along the Atlantic shores of Morocco and Western Sahara, performed large scale surveys on species distribution, evaluated species abundance and frequency of parasitism and examined species genetic patterns. I further coupled biological data with upwelling indices, sea surface temperatures (SST) and the rate of SST warming. I demonstrate that strong upwelling influences abundance and distribution of intertidal rocky shore species and that upwelling cells can act as refugia from climate change by ameliorating thermal conditions. Upwelling cells also conserve the genetic diversity of the marine macroalga Fucus guiryi, promoting intraspecific genetic diversity by preserving unique genetic lineages. However, no evidence was found that upwelling affects the genetic structure for either F. guiryi or the brown mussel Perna perna. Instead, the genetic patterns presented in this thesis seem to result from a combination of species’ life history traits, population size and habitat suitability. My results also suggest that upwelling intensity affects the frequency of endolithic parasitism on the Mediterranean mussel Mytilus galloprovincialis. In times of climate change, upwelling events provide suitable environmental conditions for species to counter act climatic change. As upwelling is project to intensify in the future, its influence on benthic intertidal species might be greater than previously anticipated.
- Full Text:
- Date Issued: 2018
- Authors: Lourenço, Carla Sofia Emídio Rodrigues
- Date: 2018
- Subjects: Upwelling (Oceanography) , Intertidal organisms -- Morocco -- Atlantic Coast , Intertidal organisms -- Canary Current -- Effect of water currents on , Intertidal animals -- Canary Current -- Effect of water currents on , Intertidal animals -- Morocco -- Atlantic Coas , Mytilus galloprovincialis -- Morocco -- Atlantic Coast , Mytilus galloprovincialis -- Canary Current -- Effect of water currents on , Intertidal ecology -- Canary Current , Sea surface microlayer -- Morocco -- Atlantic Coast
- Language: English
- Type: text , Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/61614 , vital:28042
- Description: Upwelling is an oceanographic process that strongly influences coastal species and the communities they belong to. In upwelling areas, colder, denser, nutrient-rich subsurface waters are transported to the nearshore surface, replacing warmer superficial waters that are advected offshore. Such effects influence the composition and dynamics of coastal communities, for example by affecting species abundance, recruitment, dispersal and distribution. Upwelling areas are key model regions to study the responses of coastal species to climate change because they are characterized by cooler conditions and experience lower warming rates than adjacent regions. In particular, intertidal rocky shore species are ideal coastal sentinel organisms to study distributional changes driven by climate warming because they inhabit the interface between marine and terrestrial habitats and are exposed to extremely severe environmental conditions. In fact, sharp distributional shifts have been reported for multiple intertidal species as a response to ocean warming. Although some studies have investigated the role of upwelling in influencing abundance and distribution of intertidal species, little is known about its potential as refugia against climate warming and the degree to which upwelling shapes species genetic structure is yet not fully understood. The aim of this thesis is to understand the influence of the Canary Current upwelling system on intertidal community composition, including species distribution and the genetic structure of intertidal species under current climate change. To do this, I investigated community structure of intertidal assemblages along the Atlantic shores of Morocco and Western Sahara, performed large scale surveys on species distribution, evaluated species abundance and frequency of parasitism and examined species genetic patterns. I further coupled biological data with upwelling indices, sea surface temperatures (SST) and the rate of SST warming. I demonstrate that strong upwelling influences abundance and distribution of intertidal rocky shore species and that upwelling cells can act as refugia from climate change by ameliorating thermal conditions. Upwelling cells also conserve the genetic diversity of the marine macroalga Fucus guiryi, promoting intraspecific genetic diversity by preserving unique genetic lineages. However, no evidence was found that upwelling affects the genetic structure for either F. guiryi or the brown mussel Perna perna. Instead, the genetic patterns presented in this thesis seem to result from a combination of species’ life history traits, population size and habitat suitability. My results also suggest that upwelling intensity affects the frequency of endolithic parasitism on the Mediterranean mussel Mytilus galloprovincialis. In times of climate change, upwelling events provide suitable environmental conditions for species to counter act climatic change. As upwelling is project to intensify in the future, its influence on benthic intertidal species might be greater than previously anticipated.
- Full Text:
- Date Issued: 2018
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