High performance liquid chromatographic analysis of oleandomycin in serum and urine
- Authors: Stubbs, Christopher , Haigh, John M , Kanfer, Isadore
- Date: 1986
- Language: English
- Type: text , Article
- Identifier: vital:6429 , http://hdl.handle.net/10962/d1006590
- Description: The determination of oleandomycin in serum and urine by high-performance liquid chromatography using erythromycin as internal standard is described. The separation was achieved on a reversed-phase C 1 s column employing acetonitrile-0.05 A4 phosphate buffer (30:70), adjusted to pH 7.0, as the mobile phase with UV detection at 200 nm. A solid-phase extraction procedure, combined with a simple phaseseparation step was used prior to chromatographic analysis. Linear calibration curves were obtained in the concentration ranges 0.25-5.0 pg/ml (serum) and 1 .O-25.0 pg/ml (urine). Precise quantitative analysis has been achieved at these levels with relative standard deviations of < 5%.
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Reply to correspondence: P.M. Gaylarde (1986) The human skin blanching assay—use and abuse
- Authors: Haigh, John M , Kanfer, Isadore , Meyer, Eric , Smith, Eric W
- Date: 1986
- Language: English
- Type: Article
- Identifier: vital:6376 , http://hdl.handle.net/10962/d1006293
- Description: Finally, we would like to assure Dr Gaylarde that we do not advocate the use of the human skin blanching assay. There are several other in vivo methods for determining corticosteroid activity which will provide equally meaningful results. What we are advocating is that if the human skin blanching assay is going to be used, then it should be used properly.
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Soft believers and hard unbelievers in the Xhosa cattle-killing
- Authors: Peires, Jeffrey B
- Date: 1986
- Language: English
- Type: Article
- Identifier: vital:6153 , http://hdl.handle.net/10962/d1007064 , http://dx.doi.org/10.1017/S0021853700023264
- Description: A substantial minority, perhaps 15 per cent of all Xhosa, refused to obey the prophetess Nongqawuse's orders to kill their cattle and destroy their corn. This divided Xhosaland into two parties, the amathamba (‘soft’ ones, or believers) and the amagogotya (‘hard’ ones, or unbelievers). The affiliation of individuals was partly determined by a number of factors – lungsickness in cattle, political attitude towards the Cape Colony, religious beliefs, kinship, age and gender – but a systematic analysis of each of these factors in turn suggests that none of them was sufficiently important to constitute the basis of either party. The key to understanding the division lies in an analysis of the indigenous Xhosa terms ‘soft’ and ‘hard’. ‘Softness’ in Xhosa denotes the submissiveness of the individual to the common will of the community, whereas ‘hardness’ denotes the determination of the individual to pursue his own ends, even at communal expense. Translated into social terms, the ‘soft’ believers were those who remained committed to the mutual aid ethic of the declining precolonial society, whereas the ‘hard’ unbelievers were those who sought to seize advantage of the new opportunities offered by the colonial presence to increase their wealth and social prominence. The conflict between the social and personal imperatives was well expressed by Chief Smith Mhala, the unbelieving son of a believing father, when he said, ‘They say I am killing my father – so I would kill him before I would kill my cattle.’ Certainly, the division between amathamba and amagogotya ran much deeper than the division between belief and unbelief, and the Xhosa, in conferring these names, seem to have recognized the fact.
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