Polymerized serum albumin beads for use as slow-release adjuvants
- Martin, Michelle Elizabeth Denny
- Authors: Martin, Michelle Elizabeth Denny
- Date: 1988
- Subjects: Serum albumin , Antigens , Vaccines
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3879 , http://hdl.handle.net/10962/d1001613
- Description: Experimental vaccines have been made by covalently bonding virus particles into polymerized rabbit serum albumin beads. Using Nodamura virus as a model antigen, these model vaccines induced specific humoral antibody production, comparable with that achieved using Freund's adjuvants. Virus specific antibodies were also induced when Nodamura virus was covalently attached to the bead surface using different crosslinkers. However, when poliovirus type 2 (Sabin strain) was polymerized into beads, the levels of neutralizing antibodies were insignificant compared with control aqueous vaccines. The synthetic immunostimulator, muramyl dipeptide, was included with bead vaccines in an attempt to potentiate the immune response. Immunostimulation is achieved by a slow release of antigen coinciding with the gradual breakdown of bead structure.
- Full Text:
- Authors: Martin, Michelle Elizabeth Denny
- Date: 1988
- Subjects: Serum albumin , Antigens , Vaccines
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3879 , http://hdl.handle.net/10962/d1001613
- Description: Experimental vaccines have been made by covalently bonding virus particles into polymerized rabbit serum albumin beads. Using Nodamura virus as a model antigen, these model vaccines induced specific humoral antibody production, comparable with that achieved using Freund's adjuvants. Virus specific antibodies were also induced when Nodamura virus was covalently attached to the bead surface using different crosslinkers. However, when poliovirus type 2 (Sabin strain) was polymerized into beads, the levels of neutralizing antibodies were insignificant compared with control aqueous vaccines. The synthetic immunostimulator, muramyl dipeptide, was included with bead vaccines in an attempt to potentiate the immune response. Immunostimulation is achieved by a slow release of antigen coinciding with the gradual breakdown of bead structure.
- Full Text:
Structural studies on some capsular antigens from escherichia coli and klebsiella
- Authors: Anderson, Andrew Nixon
- Date: 1988
- Subjects: Escherichia , Klebsiella , Antigens
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3730 , http://hdl.handle.net/10962/d1001469
- Description: A review of the structural studies of bacterial capsular polysaccharides (K-antigens) from Escherichia coli and Klebsiella, and of the trends in modern chemical and instrumental techniques available for the analysis of carbohydrate material is presented. The structural elucidations of the capsular polysaccharides from E. coli K37 and K55, and Klebsiella K39 are reported with comments on the novelty and possible immunological significance of the structures. The usefulness of the bacteriophage degradation technique has been emphasized using the polysaccharides from E. coli K55, and Klebsiella K30 and K39 to demonstrate the scope of the reaction
- Full Text:
- Authors: Anderson, Andrew Nixon
- Date: 1988
- Subjects: Escherichia , Klebsiella , Antigens
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3730 , http://hdl.handle.net/10962/d1001469
- Description: A review of the structural studies of bacterial capsular polysaccharides (K-antigens) from Escherichia coli and Klebsiella, and of the trends in modern chemical and instrumental techniques available for the analysis of carbohydrate material is presented. The structural elucidations of the capsular polysaccharides from E. coli K37 and K55, and Klebsiella K39 are reported with comments on the novelty and possible immunological significance of the structures. The usefulness of the bacteriophage degradation technique has been emphasized using the polysaccharides from E. coli K55, and Klebsiella K30 and K39 to demonstrate the scope of the reaction
- Full Text:
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