Predicting geographic distribution of seven blowfly species (Diptera: Calliphoridae) in South Africa
- Richards, Cameron S, Williams, Kirstin A, Villet, Martin H
- Authors: Richards, Cameron S , Williams, Kirstin A , Villet, Martin H
- Date: 2009
- Language: English
- Type: text , Article
- Identifier: vital:6854 , http://hdl.handle.net/10962/d1011135
- Description: The predicted geographic distributions of seven forensically important blowfly species are modelled using the computer program Maxent, based on selected climatic variables for South Africa, a country with large climatic and environmental gradients. It is shown that although temperature was hypothesized to most influence the distributions of these ectotherms, moisture, and particularly humidity, was in fact usually paramount. Chrysomya albiceps (Wiedemann) and C. marginalis (Robineau-Desvoidy) had the most widespread geographic and climatic distribution, while the forest-associated C. inclinata(Walker) was the least widespread. Chrysomya putoria (Wiedemann) and C. megacephala (Fabricius) had very similar predicted distributions that were restricted mainly to Limpopo, Mpumalanga, KwaZulu-Natal and the coast of the Eastern Cape. Chrysomya chloropyga (Wiedemann) and Calliphora croceipalpis (Jaennicke) were the only species predicted to occur at high altitudes. Blowfly distributions restricted to part of the map area were predicted better than those that were more widespread in the region, presumably because species with extremely widespread distributions in a study area occupy nearly the whole range of variation of most predictor variables, leaving little variation with which themaximumentropy modelling method can discriminate between presence and absence of the organism.
- Full Text:
Predicting geographic distribution of seven blowfly species (Diptera: Calliphoridae) in South Africa
- Authors: Richards, Cameron S , Williams, Kirstin A , Villet, Martin H
- Date: 2009
- Language: English
- Type: text , Article
- Identifier: vital:6854 , http://hdl.handle.net/10962/d1011135
- Description: The predicted geographic distributions of seven forensically important blowfly species are modelled using the computer program Maxent, based on selected climatic variables for South Africa, a country with large climatic and environmental gradients. It is shown that although temperature was hypothesized to most influence the distributions of these ectotherms, moisture, and particularly humidity, was in fact usually paramount. Chrysomya albiceps (Wiedemann) and C. marginalis (Robineau-Desvoidy) had the most widespread geographic and climatic distribution, while the forest-associated C. inclinata(Walker) was the least widespread. Chrysomya putoria (Wiedemann) and C. megacephala (Fabricius) had very similar predicted distributions that were restricted mainly to Limpopo, Mpumalanga, KwaZulu-Natal and the coast of the Eastern Cape. Chrysomya chloropyga (Wiedemann) and Calliphora croceipalpis (Jaennicke) were the only species predicted to occur at high altitudes. Blowfly distributions restricted to part of the map area were predicted better than those that were more widespread in the region, presumably because species with extremely widespread distributions in a study area occupy nearly the whole range of variation of most predictor variables, leaving little variation with which themaximumentropy modelling method can discriminate between presence and absence of the organism.
- Full Text:
Preservation or exploitation? : a study of the development of the mining rights legislation on the Witwatersrand goldfields from 1886 to 2008
- Authors: Stott, Joan
- Date: 2009
- Subjects: Ostrom, Elinor Chamber of Mines of South Africa Gold mines and mining -- South Africa -- Witwatersrand -- History Mineral industries -- Finance -- South Africa Mining law -- South Africa
- Language: English
- Type: Thesis , Masters , MEcon
- Identifier: vital:988 , http://hdl.handle.net/10962/d1002723
- Description: Elinor Ostrom (2005: 238) assumes that in understanding the make up and behaviour of institutional systems governing natural resources: “Resource users are explicitly thought of as rational egoists who plunder local resources so as to maximise their own short-term benefits. Government officials are implicitly depicted, on the other hand, as seeking, the more general public interest, having the relevant information at hand and the capability of designing optimal policies.” This thesis examines the validity of this assumption through an historical analysis of the deep-level gold mining industry of the Witwatersrand, South Africa. The main focus of the assessment is on the institutions of ownership – that is, the development of mining rights and title legislation between 1886 and 2008. The study looks at the legislations’ transformation and implementation from the perspective of the gold mining industry – made up of the mining finance houses and the Chamber of Mines of South Africa – and that of the state. The transformation of the mining industry’s institutional framework was both a choice by government as well as that of the firms in the mining industry. The theoretical framework is constructed from four areas of economic thought. These include: the neoclassical and Keynesian schools of macroeconomic thought; industrial organisation and its relevance to the relationship between firms and the market; institutional and new institutional economics; and finally property rights. The determinants of policy design and the impact of such design on firms and industry is examined. The development, implementation and use of the aforementioned legislation is examined from two perspectives, namely, that of preserver or exploiter. Throughout the history of this prominent South African industry, the motivation for action from the industry or government has oscillated between the two extremes of preserver or exploiter over the time period examined. The conclusion is drawn on an overall and broad focus of actions – with a strong focus on the most recent developments in mining legislation – post-1992.
- Full Text:
- Authors: Stott, Joan
- Date: 2009
- Subjects: Ostrom, Elinor Chamber of Mines of South Africa Gold mines and mining -- South Africa -- Witwatersrand -- History Mineral industries -- Finance -- South Africa Mining law -- South Africa
- Language: English
- Type: Thesis , Masters , MEcon
- Identifier: vital:988 , http://hdl.handle.net/10962/d1002723
- Description: Elinor Ostrom (2005: 238) assumes that in understanding the make up and behaviour of institutional systems governing natural resources: “Resource users are explicitly thought of as rational egoists who plunder local resources so as to maximise their own short-term benefits. Government officials are implicitly depicted, on the other hand, as seeking, the more general public interest, having the relevant information at hand and the capability of designing optimal policies.” This thesis examines the validity of this assumption through an historical analysis of the deep-level gold mining industry of the Witwatersrand, South Africa. The main focus of the assessment is on the institutions of ownership – that is, the development of mining rights and title legislation between 1886 and 2008. The study looks at the legislations’ transformation and implementation from the perspective of the gold mining industry – made up of the mining finance houses and the Chamber of Mines of South Africa – and that of the state. The transformation of the mining industry’s institutional framework was both a choice by government as well as that of the firms in the mining industry. The theoretical framework is constructed from four areas of economic thought. These include: the neoclassical and Keynesian schools of macroeconomic thought; industrial organisation and its relevance to the relationship between firms and the market; institutional and new institutional economics; and finally property rights. The determinants of policy design and the impact of such design on firms and industry is examined. The development, implementation and use of the aforementioned legislation is examined from two perspectives, namely, that of preserver or exploiter. Throughout the history of this prominent South African industry, the motivation for action from the industry or government has oscillated between the two extremes of preserver or exploiter over the time period examined. The conclusion is drawn on an overall and broad focus of actions – with a strong focus on the most recent developments in mining legislation – post-1992.
- Full Text:
Promoting indigenous vegetables in urban agriculture & livelihoods : policy lessons from Sub-Saharan Africa
- Shackleton, Charlie M, Pasquini, Margaret W, Ambrose-Oji, Bianca, Drescher, Axel W
- Authors: Shackleton, Charlie M , Pasquini, Margaret W , Ambrose-Oji, Bianca , Drescher, Axel W
- Date: 2009
- Language: English
- Type: Article
- Identifier: vital:6619 , http://hdl.handle.net/10962/d1016227
- Description: [From Introduction] Globally, the growth of urbanised areas continues at an exponential rate, and most spectacularly in the developing world. The global urban population will increase from 2.9 billion in 2000 to 5.0 billion by 2030. The mean rate of urban growth in non-OECD countries between 2000 and 2005 was just under 3% per annum, compared to 0.5 % for rural regions of the same countries (UN-Habitat 2006). Although the proportion of Africans currently living in urban areas is the lowest in the world (+ 40%), because of this low base it is not unsurprising that the rates of urbanisation are among the highest at approximately 4.3% per annum. Projections vary, but sometime in the mid- 2020s over 50% of Africa's population will be living in urban areas, as compared to just 15% in 1950 and 34% in 1994. As urbanisation takes place another important trend is revealed, namely the locus of poverty in Africa is slowly shifting from rural to urban areas. For example, it is estimated that more than 56% of the world's absolute or chronic poor will be concentrated in urban areas (WRI 1996). Since as much as 60-80% of the income of the urban poor is spent on the purchase of food (Maxwell et al. 2000), the issue of food supply, both its quantity and quality, is increasingly a central issue in poverty reduction debates and strategies. In rural areas, a common strategy to alleviate poverty is to call for measures to boost small-holder food production. Surprisingly, this is less common in urban poverty alleviation programmes, despite the widespread promise of urban and household agriculture in contributing to improved food security.
- Full Text:
- Authors: Shackleton, Charlie M , Pasquini, Margaret W , Ambrose-Oji, Bianca , Drescher, Axel W
- Date: 2009
- Language: English
- Type: Article
- Identifier: vital:6619 , http://hdl.handle.net/10962/d1016227
- Description: [From Introduction] Globally, the growth of urbanised areas continues at an exponential rate, and most spectacularly in the developing world. The global urban population will increase from 2.9 billion in 2000 to 5.0 billion by 2030. The mean rate of urban growth in non-OECD countries between 2000 and 2005 was just under 3% per annum, compared to 0.5 % for rural regions of the same countries (UN-Habitat 2006). Although the proportion of Africans currently living in urban areas is the lowest in the world (+ 40%), because of this low base it is not unsurprising that the rates of urbanisation are among the highest at approximately 4.3% per annum. Projections vary, but sometime in the mid- 2020s over 50% of Africa's population will be living in urban areas, as compared to just 15% in 1950 and 34% in 1994. As urbanisation takes place another important trend is revealed, namely the locus of poverty in Africa is slowly shifting from rural to urban areas. For example, it is estimated that more than 56% of the world's absolute or chronic poor will be concentrated in urban areas (WRI 1996). Since as much as 60-80% of the income of the urban poor is spent on the purchase of food (Maxwell et al. 2000), the issue of food supply, both its quantity and quality, is increasingly a central issue in poverty reduction debates and strategies. In rural areas, a common strategy to alleviate poverty is to call for measures to boost small-holder food production. Surprisingly, this is less common in urban poverty alleviation programmes, despite the widespread promise of urban and household agriculture in contributing to improved food security.
- Full Text:
Protein secondary structure prediction using neural networks and support vector machines
- Authors: Tsilo, Lipontseng Cecilia
- Date: 2009
- Subjects: Neural networks (Computer science) , Support vector machines , Proteins -- Structure -- Mathematical models
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5569 , http://hdl.handle.net/10962/d1002809 , Neural networks (Computer science) , Support vector machines , Proteins -- Structure -- Mathematical models
- Description: Predicting the secondary structure of proteins is important in biochemistry because the 3D structure can be determined from the local folds that are found in secondary structures. Moreover, knowing the tertiary structure of proteins can assist in determining their functions. The objective of this thesis is to compare the performance of Neural Networks (NN) and Support Vector Machines (SVM) in predicting the secondary structure of 62 globular proteins from their primary sequence. For each NN and SVM, we created six binary classifiers to distinguish between the classes’ helices (H) strand (E), and coil (C). For NN we use Resilient Backpropagation training with and without early stopping. We use NN with either no hidden layer or with one hidden layer with 1,2,...,40 hidden neurons. For SVM we use a Gaussian kernel with parameter fixed at = 0.1 and varying cost parameters C in the range [0.1,5]. 10- fold cross-validation is used to obtain overall estimates for the probability of making a correct prediction. Our experiments indicate for NN and SVM that the different binary classifiers have varying accuracies: from 69% correct predictions for coils vs. non-coil up to 80% correct predictions for stand vs. non-strand. It is further demonstrated that NN with no hidden layer or not more than 2 hidden neurons in the hidden layer are sufficient for better predictions. For SVM we show that the estimated accuracies do not depend on the value of the cost parameter. As a major result, we will demonstrate that the accuracy estimates of NN and SVM binary classifiers cannot distinguish. This contradicts a modern belief in bioinformatics that SVM outperforms other predictors.
- Full Text:
- Authors: Tsilo, Lipontseng Cecilia
- Date: 2009
- Subjects: Neural networks (Computer science) , Support vector machines , Proteins -- Structure -- Mathematical models
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5569 , http://hdl.handle.net/10962/d1002809 , Neural networks (Computer science) , Support vector machines , Proteins -- Structure -- Mathematical models
- Description: Predicting the secondary structure of proteins is important in biochemistry because the 3D structure can be determined from the local folds that are found in secondary structures. Moreover, knowing the tertiary structure of proteins can assist in determining their functions. The objective of this thesis is to compare the performance of Neural Networks (NN) and Support Vector Machines (SVM) in predicting the secondary structure of 62 globular proteins from their primary sequence. For each NN and SVM, we created six binary classifiers to distinguish between the classes’ helices (H) strand (E), and coil (C). For NN we use Resilient Backpropagation training with and without early stopping. We use NN with either no hidden layer or with one hidden layer with 1,2,...,40 hidden neurons. For SVM we use a Gaussian kernel with parameter fixed at = 0.1 and varying cost parameters C in the range [0.1,5]. 10- fold cross-validation is used to obtain overall estimates for the probability of making a correct prediction. Our experiments indicate for NN and SVM that the different binary classifiers have varying accuracies: from 69% correct predictions for coils vs. non-coil up to 80% correct predictions for stand vs. non-strand. It is further demonstrated that NN with no hidden layer or not more than 2 hidden neurons in the hidden layer are sufficient for better predictions. For SVM we show that the estimated accuracies do not depend on the value of the cost parameter. As a major result, we will demonstrate that the accuracy estimates of NN and SVM binary classifiers cannot distinguish. This contradicts a modern belief in bioinformatics that SVM outperforms other predictors.
- Full Text:
Pseudopolydora species associated with mollusc shells on the south coast of South Africa, with the description of Ps. dayii, sp nov
- Authors: Simon, Carol A
- Date: 2009
- Language: English
- Type: text , Article
- Identifier: vital:6875 , http://hdl.handle.net/10962/d1011623
- Description: Two species of the genus Pseudopolydora, Ps. dayii, sp. nov. and Ps. antennata, were associated with gastropods on the south and south-east coasts of South Africa. Pseudopolydora dayii is characterized by prominent post-chaetal notopodial lobes on chaetiger 1 with very long chaetae, a prominent occipital tentacle, having hooded hooks that start on chaetiger 9, branchiae that start on chaetiger 6, stout hooks and lobes placed latero-posteriorly to the hooded hooks in posterior chaetigers. It is a surface-fouler and was found on several species of wild gastropods at four of the five sites sampled and from additional material from the south-west coast and on cultured abalone (Haliotis midae) at a farm on the south-west coast. Three individuals of Ps. antennata were found only with oysters at the easternmost site. This was the first record of this species outside of the Western Cape Province and it is possible that their association with the oysters was fortuitous.
- Full Text:
- Authors: Simon, Carol A
- Date: 2009
- Language: English
- Type: text , Article
- Identifier: vital:6875 , http://hdl.handle.net/10962/d1011623
- Description: Two species of the genus Pseudopolydora, Ps. dayii, sp. nov. and Ps. antennata, were associated with gastropods on the south and south-east coasts of South Africa. Pseudopolydora dayii is characterized by prominent post-chaetal notopodial lobes on chaetiger 1 with very long chaetae, a prominent occipital tentacle, having hooded hooks that start on chaetiger 9, branchiae that start on chaetiger 6, stout hooks and lobes placed latero-posteriorly to the hooded hooks in posterior chaetigers. It is a surface-fouler and was found on several species of wild gastropods at four of the five sites sampled and from additional material from the south-west coast and on cultured abalone (Haliotis midae) at a farm on the south-west coast. Three individuals of Ps. antennata were found only with oysters at the easternmost site. This was the first record of this species outside of the Western Cape Province and it is possible that their association with the oysters was fortuitous.
- Full Text:
Public discourses on choice of termination of pregnancy in a rural area of the Eastern Cape Province in South Africa
- Authors: Sigcau, Nomakhosi
- Date: 2009
- Subjects: Abortion -- South Africa -- Eastern Cape Abortion -- Public opinion Abortion -- Moral and ethical aspects Abortion -- Social aspects Abortion -- Psychological aspects Discourse analysis -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3055 , http://hdl.handle.net/10962/d1002564
- Description: A period of ten years has elapsed since the Choice on Termination of Pregnancy Act came into effect. Little has been done in South Africa to investigate public discourses concerning termination of pregnancy since the implementation of the Act. The social context and the quality of available support systems determine the outcome of the women’s feelings after the abortion. Knowledge about the social context is important, as it will help to understand the complexities and nuances of abortion. The aim of the research is to explore public discourses on Choice on Termination of Pregnancy (CTOP), and the potential implications of these discourses on the use of the CTOP service. The sample consisted of 23 black isiXhosa-speaking participants from the rural area of the Eastern Cape Province in South Africa. Four focus groups coming from different age groups (between the ages 18 and 52) with both men and women participated in the study. Fictitious vignettes that tap into two different scenarios regarding abortion based on women’s stories were used. Discourses that emerged from people’s text are explained, described and interpreted through a discourse analysis. Since the study was interested in public discourses it led to the discovery of 17 interpretative repertoires as follows: social stigma, abortion equated to murder, degradation of society, pregnancy as an irresponsible act, conditional acceptance, TOP in the context of marriage, future potentiality invested in the foetus, dehumanizing foetus into a clot, shared decision making responsibility, gender dynamics interpretative repertoire, negative post abortion consequences, the scolding versus the supportive nurse interpretative repertoire, alternatives interpretative repertoire, rights versus no responsibility interpretative repertoire, more knowledge needed, male and female or generational differentiation repertoire, and the positive effects repertoire. Abortion is opposed on religious and cultural grounds. TOP has been legalized in South Africa but with this a debate and conflicting views have arisen. These variations in people’s discourses may limit access to TOP for women who need the service.
- Full Text:
- Authors: Sigcau, Nomakhosi
- Date: 2009
- Subjects: Abortion -- South Africa -- Eastern Cape Abortion -- Public opinion Abortion -- Moral and ethical aspects Abortion -- Social aspects Abortion -- Psychological aspects Discourse analysis -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3055 , http://hdl.handle.net/10962/d1002564
- Description: A period of ten years has elapsed since the Choice on Termination of Pregnancy Act came into effect. Little has been done in South Africa to investigate public discourses concerning termination of pregnancy since the implementation of the Act. The social context and the quality of available support systems determine the outcome of the women’s feelings after the abortion. Knowledge about the social context is important, as it will help to understand the complexities and nuances of abortion. The aim of the research is to explore public discourses on Choice on Termination of Pregnancy (CTOP), and the potential implications of these discourses on the use of the CTOP service. The sample consisted of 23 black isiXhosa-speaking participants from the rural area of the Eastern Cape Province in South Africa. Four focus groups coming from different age groups (between the ages 18 and 52) with both men and women participated in the study. Fictitious vignettes that tap into two different scenarios regarding abortion based on women’s stories were used. Discourses that emerged from people’s text are explained, described and interpreted through a discourse analysis. Since the study was interested in public discourses it led to the discovery of 17 interpretative repertoires as follows: social stigma, abortion equated to murder, degradation of society, pregnancy as an irresponsible act, conditional acceptance, TOP in the context of marriage, future potentiality invested in the foetus, dehumanizing foetus into a clot, shared decision making responsibility, gender dynamics interpretative repertoire, negative post abortion consequences, the scolding versus the supportive nurse interpretative repertoire, alternatives interpretative repertoire, rights versus no responsibility interpretative repertoire, more knowledge needed, male and female or generational differentiation repertoire, and the positive effects repertoire. Abortion is opposed on religious and cultural grounds. TOP has been legalized in South Africa but with this a debate and conflicting views have arisen. These variations in people’s discourses may limit access to TOP for women who need the service.
- Full Text:
Purification and characterization of fructosyltransferase for the synthesis of short-chain fructo-oligosaccharides and investigation into thier anti-carcinogenic properties
- Authors: Nemukula, Aluwani
- Date: 2009
- Subjects: Oligosaccharides , Polygalacturonase , Aspergillus , Fructose , Inulin , Cancer -- Prevention , Cancer -- Research , Carcinogens , High performance liquid chromatography
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3927 , http://hdl.handle.net/10962/d1003986 , Oligosaccharides , Polygalacturonase , Aspergillus , Fructose , Inulin , Cancer -- Prevention , Cancer -- Research , Carcinogens , High performance liquid chromatography
- Description: There is a growing attention in the synthesis of fructo-oligosaccharides (FOS) due to their excellent bio-functional and health-promoting properties. The current production processes are limited to chemical hydrolysis reactions of plant extracts, which are often associated with several drawbacks. In this study, fructosyltransferase (FTase) and polygalacturonase (PGase) activities, present in a commercial enzyme preparation (Pectinex® Ultra SP-L) sourced from Aspergillus aculeatus, have been separated and fully purified by anion-exchange and sizeexclusion chromatography. The FTase possesses fructosyl transfer activity for FOS synthesis and the PGase has pectin hydrolytic activity. Fructosyltransferase is a single-band protein with a molecular weight of 85 kDa, whereas PGase is a distinct protein of 40 kDa. The temperature and pH optima of FTase were 60 ºC and 6.0, with a half-life of 8 h; while that for PGase were 40 ºC and 6.0, respectively. FTase was slightly inhibited in the presence of Ni²⁺, Mg²⁺ and urea; but PGase was more susceptible to divalent ions such as Ca²⁺, Mg²⁺ and Mn²⁺. The kinetic parameters (Km and Vmax) of FTase for the hydrolysis of β-(2→1) linkages from sucrose were 752.3 mM and 120.5 μmol.min⁻¹.mL⁻¹, respectively; whereas the same parameters for pectin hydrolysis by PGase were 13.0 mg.mL⁻¹ and 263 μmol.min-1.mL⁻¹, respectively. The purified FTase was able to transfer fructosyl residues from sucrose, synthesizing the corresponding chains of FOS. PGase was relatively stable at 40 ºC (t½ > 3 h), depolymerizing the pectin backbone while releasing the inulins from within the chicory roots. Analysis of various mixtures of FOS by mass spectrometry, HPLC and ¹H-NMR was undertaken. Results indicated that MS with electrospray ionization and ¹H-NMR are capable of providing relative quantitative data of the FOS present in the mixtures. The pharmaceutical effects of various sc-FOS (0.5%, v/v) and SCFA (0.3%, v/v) on certain bacterial enzymes (β-glucuronidase, urease and β-glucosidase) associated with the formation of carcinogens were also studied. These enzyme activities were not directly influenced by the sc-FOS, but were found to be remarkably decreased by SCFA, pointing toward the prebiotic effect of FOS in intestinal microflora modulation.
- Full Text:
- Authors: Nemukula, Aluwani
- Date: 2009
- Subjects: Oligosaccharides , Polygalacturonase , Aspergillus , Fructose , Inulin , Cancer -- Prevention , Cancer -- Research , Carcinogens , High performance liquid chromatography
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3927 , http://hdl.handle.net/10962/d1003986 , Oligosaccharides , Polygalacturonase , Aspergillus , Fructose , Inulin , Cancer -- Prevention , Cancer -- Research , Carcinogens , High performance liquid chromatography
- Description: There is a growing attention in the synthesis of fructo-oligosaccharides (FOS) due to their excellent bio-functional and health-promoting properties. The current production processes are limited to chemical hydrolysis reactions of plant extracts, which are often associated with several drawbacks. In this study, fructosyltransferase (FTase) and polygalacturonase (PGase) activities, present in a commercial enzyme preparation (Pectinex® Ultra SP-L) sourced from Aspergillus aculeatus, have been separated and fully purified by anion-exchange and sizeexclusion chromatography. The FTase possesses fructosyl transfer activity for FOS synthesis and the PGase has pectin hydrolytic activity. Fructosyltransferase is a single-band protein with a molecular weight of 85 kDa, whereas PGase is a distinct protein of 40 kDa. The temperature and pH optima of FTase were 60 ºC and 6.0, with a half-life of 8 h; while that for PGase were 40 ºC and 6.0, respectively. FTase was slightly inhibited in the presence of Ni²⁺, Mg²⁺ and urea; but PGase was more susceptible to divalent ions such as Ca²⁺, Mg²⁺ and Mn²⁺. The kinetic parameters (Km and Vmax) of FTase for the hydrolysis of β-(2→1) linkages from sucrose were 752.3 mM and 120.5 μmol.min⁻¹.mL⁻¹, respectively; whereas the same parameters for pectin hydrolysis by PGase were 13.0 mg.mL⁻¹ and 263 μmol.min-1.mL⁻¹, respectively. The purified FTase was able to transfer fructosyl residues from sucrose, synthesizing the corresponding chains of FOS. PGase was relatively stable at 40 ºC (t½ > 3 h), depolymerizing the pectin backbone while releasing the inulins from within the chicory roots. Analysis of various mixtures of FOS by mass spectrometry, HPLC and ¹H-NMR was undertaken. Results indicated that MS with electrospray ionization and ¹H-NMR are capable of providing relative quantitative data of the FOS present in the mixtures. The pharmaceutical effects of various sc-FOS (0.5%, v/v) and SCFA (0.3%, v/v) on certain bacterial enzymes (β-glucuronidase, urease and β-glucosidase) associated with the formation of carcinogens were also studied. These enzyme activities were not directly influenced by the sc-FOS, but were found to be remarkably decreased by SCFA, pointing toward the prebiotic effect of FOS in intestinal microflora modulation.
- Full Text:
Putting the ‘T’ into South African human rights: transsexuality in the post-apartheid order
- Vincent, Louise, Camminga, Bianca
- Authors: Vincent, Louise , Camminga, Bianca
- Date: 2009
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/141606 , vital:37989 , DOI: 10.1177/1363460709346108
- Description: Informed by narratives provided by self-identified South African transsexuals, whose lives span different periods of South Africa’s political and social history, this article seeks to explore how South Africa’s medical, legal and military establishments have exerted power over the transsexual body. A variety of studies outline the extent to which the apartheid state was a highly gendered state characterized by inflexible patriarchal norms and the dominance of violent and authoritarian forms of masculine expression. Hyper masculinization and militarization were explicit goals of the apartheid state. Deviance from the state’s prescribed gender norms was not simply socially unacceptable, it was, in many cases, punishable. South Africa’s post-1994 democratic Constitution, in contrast, explicitly outlaws discrimination on the basis of sexual orientation. But the democratic legal framework, which provides significant protections for freedom of sexual expression and freedom from discrimination for homosexuals has arguably had less of an impact on the lives of South Africa’s transsexual community. The state, even the post-apartheid state, has been loathe to move beyond the idea of a necessary correlation between the physical make-up of the body and the gender identity of a person in the way in which it has treated the idea of transsexualism.
- Full Text:
- Authors: Vincent, Louise , Camminga, Bianca
- Date: 2009
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/141606 , vital:37989 , DOI: 10.1177/1363460709346108
- Description: Informed by narratives provided by self-identified South African transsexuals, whose lives span different periods of South Africa’s political and social history, this article seeks to explore how South Africa’s medical, legal and military establishments have exerted power over the transsexual body. A variety of studies outline the extent to which the apartheid state was a highly gendered state characterized by inflexible patriarchal norms and the dominance of violent and authoritarian forms of masculine expression. Hyper masculinization and militarization were explicit goals of the apartheid state. Deviance from the state’s prescribed gender norms was not simply socially unacceptable, it was, in many cases, punishable. South Africa’s post-1994 democratic Constitution, in contrast, explicitly outlaws discrimination on the basis of sexual orientation. But the democratic legal framework, which provides significant protections for freedom of sexual expression and freedom from discrimination for homosexuals has arguably had less of an impact on the lives of South Africa’s transsexual community. The state, even the post-apartheid state, has been loathe to move beyond the idea of a necessary correlation between the physical make-up of the body and the gender identity of a person in the way in which it has treated the idea of transsexualism.
- Full Text:
Quantitative post-release evaluation of biological control of floating fern, Salvinia molesta DS Mitchell (Salviniaceae), with Cyrtobagous salviniae Calder and Sands (Coleoptera Curculionidae) on the Senegal River and Senegal River Delta
- Diop Ousseynou, Hill, Martin P
- Authors: Diop Ousseynou , Hill, Martin P
- Date: 2009
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/451467 , vital:75051 , https://hdl.handle.net/10520/EJC32802
- Description: The floating aquatic fern, Salvinia molesta D.S Mitchell (Salviniaceae) was first recorded in Senegal in 1999 and by 2000 it covered an estimated area of 18 000 ha on the Senegal River Left Bank and tributaries (Senegal) and 7840 ha on the Senegal River Right Bank (Mauritania). The weevil, Cyrtobagous salviniae Calder and Sands (Coleoptera: Curculionidae) was considered the best option for control and some 48 953 weevils were released at 270 sites during 2002. Within one year, weevils had established and were recovered up to 50km from the release sites. At most of the release sites, the S. molesta infestation was reduced from 100 % to less than 5 % within 24 months of release. These results are discussed in the context of the weed's negative impact on aquatic systems.
- Full Text:
- Authors: Diop Ousseynou , Hill, Martin P
- Date: 2009
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/451467 , vital:75051 , https://hdl.handle.net/10520/EJC32802
- Description: The floating aquatic fern, Salvinia molesta D.S Mitchell (Salviniaceae) was first recorded in Senegal in 1999 and by 2000 it covered an estimated area of 18 000 ha on the Senegal River Left Bank and tributaries (Senegal) and 7840 ha on the Senegal River Right Bank (Mauritania). The weevil, Cyrtobagous salviniae Calder and Sands (Coleoptera: Curculionidae) was considered the best option for control and some 48 953 weevils were released at 270 sites during 2002. Within one year, weevils had established and were recovered up to 50km from the release sites. At most of the release sites, the S. molesta infestation was reduced from 100 % to less than 5 % within 24 months of release. These results are discussed in the context of the weed's negative impact on aquatic systems.
- Full Text:
Quantity and significance of wild meat off-take by a rural community in the Eastern Cape, South Africa
- Kaschula, Sarah A H, Shackleton, Charlie M
- Authors: Kaschula, Sarah A H , Shackleton, Charlie M
- Date: 2009
- Language: English
- Type: Article
- Identifier: vital:6638 , http://hdl.handle.net/10962/d1006864
- Description: When compared to tropical forest zones in west and central Africa, off-take of wild meat from savannah and grassland biomes by local rural communities has not been well assessed. This case study of wild meat collection activities within a rural community in the Mount Frere region of the Eastern Cape (South Africa) uses last-catch records derived from 50 wild meat gatherers to calculate average off-take of taxa, species and fresh mass of wild meat per collection event. When per-event off take is overlaid onto household hunting frequency data, annual off-take would be 268.6 kg km−2 yr−1 or 3 kg person−1 yr−1 presuming constant off-take over an annual period. Monetary value of off-take would be South African R 307 (US$ 39) per household annually. For some species, off-take weight per km2 shows similar values to data from tropical forest zones, but high human population densities tend to dilute off-takes to less nutritionally significant amounts at the per person scale. However, unlike many tropical zones, none of the species harvested can be considered high-priority conservation species. Even densely populated and heavily harvested communal lands appear to offer high wild meat off-takes from low conservation priority species.
- Full Text:
- Authors: Kaschula, Sarah A H , Shackleton, Charlie M
- Date: 2009
- Language: English
- Type: Article
- Identifier: vital:6638 , http://hdl.handle.net/10962/d1006864
- Description: When compared to tropical forest zones in west and central Africa, off-take of wild meat from savannah and grassland biomes by local rural communities has not been well assessed. This case study of wild meat collection activities within a rural community in the Mount Frere region of the Eastern Cape (South Africa) uses last-catch records derived from 50 wild meat gatherers to calculate average off-take of taxa, species and fresh mass of wild meat per collection event. When per-event off take is overlaid onto household hunting frequency data, annual off-take would be 268.6 kg km−2 yr−1 or 3 kg person−1 yr−1 presuming constant off-take over an annual period. Monetary value of off-take would be South African R 307 (US$ 39) per household annually. For some species, off-take weight per km2 shows similar values to data from tropical forest zones, but high human population densities tend to dilute off-takes to less nutritionally significant amounts at the per person scale. However, unlike many tropical zones, none of the species harvested can be considered high-priority conservation species. Even densely populated and heavily harvested communal lands appear to offer high wild meat off-takes from low conservation priority species.
- Full Text:
Racism and law : implementing the right to equality in selected South African equality courts
- Authors: Krüger, Rósaan
- Date: 2009
- Subjects: South Africa Promotion of Equality and Prevention of Unfair Discrimination Act, 2000 , Equality -- South Africa , Discrimination -- Law and legislation -- South Africa , Racism -- Law and legislation -- South Africa , Apartheid -- Law and legislation -- South Africa , Constitutional law -- South Africa , South Africa -- Politics and government -- 1994-
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3677 , http://hdl.handle.net/10962/d1003192 , South Africa Promotion of Equality and Prevention of Unfair Discrimination Act, 2000 , Equality -- South Africa , Discrimination -- Law and legislation -- South Africa , Racism -- Law and legislation -- South Africa , Apartheid -- Law and legislation -- South Africa , Constitutional law -- South Africa , South Africa -- Politics and government -- 1994-
- Description: Racism has informed South African society since colonial times. Racist beliefs found expression in the laws of colonial and apartheid South Africa and shaped both state and society. The constitutional state that South Africa has become since 1994, is based on the values of ‘human dignity’, ‘the achievement of equality’ and ‘nonracialism’, among others. Law formed the basis of the racist state prior to 1994, and now law has a fundamental role to play in the transformation of the state and society in an egalitarian direction by addressing socio-economic inequalities on the one hand, and by changing patterns of behaviour based on racist beliefs forged in the past, on the other. This thesis examines one of the legal instruments that is intended to contribute to transformation in the latter sense, namely the Promotion of Equality and Prevention of Unfair Discrimination Act 4 of 2000 (the Equality Act), with specific reference to the issue of racism. The provisions of this Act and the framework for its operation against the background of South Africa’s racist past, and within the broader framework of international and constitutional law, are examined. These two legal frameworks are analysed for the purpose of determining the standards set by international and constitutional law regarding racial equality in order to determine whether the Equality Act measures up. This thesis also incorporates an analysis of the practical application of the provisions of the Equality Act to complaints of racism in selected equality courts. The theoretical analysis of the Act’s provisions and their application in the equality courts point to various problematic formulations and obstacles which negatively affect the application of the provisions and thus hamper social change. The thesis concludes with recommendations for refining the Act’s provisions and its application.
- Full Text:
- Authors: Krüger, Rósaan
- Date: 2009
- Subjects: South Africa Promotion of Equality and Prevention of Unfair Discrimination Act, 2000 , Equality -- South Africa , Discrimination -- Law and legislation -- South Africa , Racism -- Law and legislation -- South Africa , Apartheid -- Law and legislation -- South Africa , Constitutional law -- South Africa , South Africa -- Politics and government -- 1994-
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3677 , http://hdl.handle.net/10962/d1003192 , South Africa Promotion of Equality and Prevention of Unfair Discrimination Act, 2000 , Equality -- South Africa , Discrimination -- Law and legislation -- South Africa , Racism -- Law and legislation -- South Africa , Apartheid -- Law and legislation -- South Africa , Constitutional law -- South Africa , South Africa -- Politics and government -- 1994-
- Description: Racism has informed South African society since colonial times. Racist beliefs found expression in the laws of colonial and apartheid South Africa and shaped both state and society. The constitutional state that South Africa has become since 1994, is based on the values of ‘human dignity’, ‘the achievement of equality’ and ‘nonracialism’, among others. Law formed the basis of the racist state prior to 1994, and now law has a fundamental role to play in the transformation of the state and society in an egalitarian direction by addressing socio-economic inequalities on the one hand, and by changing patterns of behaviour based on racist beliefs forged in the past, on the other. This thesis examines one of the legal instruments that is intended to contribute to transformation in the latter sense, namely the Promotion of Equality and Prevention of Unfair Discrimination Act 4 of 2000 (the Equality Act), with specific reference to the issue of racism. The provisions of this Act and the framework for its operation against the background of South Africa’s racist past, and within the broader framework of international and constitutional law, are examined. These two legal frameworks are analysed for the purpose of determining the standards set by international and constitutional law regarding racial equality in order to determine whether the Equality Act measures up. This thesis also incorporates an analysis of the practical application of the provisions of the Equality Act to complaints of racism in selected equality courts. The theoretical analysis of the Act’s provisions and their application in the equality courts point to various problematic formulations and obstacles which negatively affect the application of the provisions and thus hamper social change. The thesis concludes with recommendations for refining the Act’s provisions and its application.
- Full Text:
Rapid biological assessment of the fishery potential of Xonxa Dam, near Queenstown, South Africa
- Richardson, T J, Booth, Anthony J, Weyl, Olaf L F
- Authors: Richardson, T J , Booth, Anthony J , Weyl, Olaf L F
- Date: 2009
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/125798 , vital:35818 , https://doi.10.2989/AJAS.2009.34.1.9.734
- Description: In Africa, the harvesting of fish from small reservoirs has been identified as an important food resource for small rural communities, particularly those living close to waterbodies (Kapetsky and Petr 1984, Marshall and Maes 1994, van der Knaap 1994). Development of fisheries to utilize these resources has recently been identified by the African Union as a priority investment area for poverty alleviation and regional economic development (NEPAD 2005). Within a South African context, it is suspected that there will be increased interest in developing these fisheries to address major national policy objectives, which include food security, economic empowerment, optimal economic benefit from water, and poverty eradication (RSA 1998a, 1998b). South Africa, however, presents a somewhat anomalous situation. The lack of a fishing history in communities, the lack of species with a high fisheries potential, inadequate inland fisheries policy and a lack of directed fisheries development have resulted in low utilisation levels of fish resources in South African reservoirs (Weyl et al. 2007).
- Full Text:
- Authors: Richardson, T J , Booth, Anthony J , Weyl, Olaf L F
- Date: 2009
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/125798 , vital:35818 , https://doi.10.2989/AJAS.2009.34.1.9.734
- Description: In Africa, the harvesting of fish from small reservoirs has been identified as an important food resource for small rural communities, particularly those living close to waterbodies (Kapetsky and Petr 1984, Marshall and Maes 1994, van der Knaap 1994). Development of fisheries to utilize these resources has recently been identified by the African Union as a priority investment area for poverty alleviation and regional economic development (NEPAD 2005). Within a South African context, it is suspected that there will be increased interest in developing these fisheries to address major national policy objectives, which include food security, economic empowerment, optimal economic benefit from water, and poverty eradication (RSA 1998a, 1998b). South Africa, however, presents a somewhat anomalous situation. The lack of a fishing history in communities, the lack of species with a high fisheries potential, inadequate inland fisheries policy and a lack of directed fisheries development have resulted in low utilisation levels of fish resources in South African reservoirs (Weyl et al. 2007).
- Full Text:
Rapid method for the quantitative determination of efavirenz in human plasma
- Kanfer, Isadore, Mogatle, Seloi
- Authors: Kanfer, Isadore , Mogatle, Seloi
- Date: 2009
- Language: English
- Type: Article
- Identifier: vital:6388 , http://hdl.handle.net/10962/d1006309
- Description: A pharmacokinetic interaction study between efavirenz (EFV), a non-nucleoside reverse transcriptase inhibitor used in the treatment of HIV-1 infection, and an African traditional medicine, African potato in human subjects was undertaken. This necessitated the development and validation of a quantitative method for the analysis of EFV in plasma. A simple mobile phase consisting of 0.1 M formic acid, acetonitrile and methanol (43:52:5) was pumped at a low flow rate of 0.3 ml/min through a reverse phase Phenomenex® Luna C18 (2) (5 μm, 150 mm × 2.0 mm i.d.) column maintained at 40 °C. Diclofenac sodium was used as an internal standard (IS) and EFV and IS were monitored at 247 nm and 275 nm, respectively. A simple and rapid sample preparation involved the addition of mobile phase to 100 μl of plasma to precipitate plasma proteins followed by direct injection of 10 μl of supernatant onto the column. The procedures were validated according to international standards with good reproducibility and linear response (r = 0.9990). The intra- and inter-day accuracies were between 12.3 and 17.7% at the LLOQ and between −5.8 and 9.1% for the QC samples. The intra- and inter-day precision of EFV determinations were 5.1 or less and 7.2% RSD or less, respectively across the entire QC concentration range. Mean recovery based on high, medium and low quality control standards ranged between 92.7 and 94.1% with %RSD values better than 3%. Plasma samples were evaluated for short-term (ambient temperature for 6 h) and long-term (−10 ± 2 °C for 60 days) storage conditions and were found to be stable. The method described is cost-effective and has the necessary accuracy and precision for the rapid quantitative determination of EFV in human plasma.
- Full Text:
- Authors: Kanfer, Isadore , Mogatle, Seloi
- Date: 2009
- Language: English
- Type: Article
- Identifier: vital:6388 , http://hdl.handle.net/10962/d1006309
- Description: A pharmacokinetic interaction study between efavirenz (EFV), a non-nucleoside reverse transcriptase inhibitor used in the treatment of HIV-1 infection, and an African traditional medicine, African potato in human subjects was undertaken. This necessitated the development and validation of a quantitative method for the analysis of EFV in plasma. A simple mobile phase consisting of 0.1 M formic acid, acetonitrile and methanol (43:52:5) was pumped at a low flow rate of 0.3 ml/min through a reverse phase Phenomenex® Luna C18 (2) (5 μm, 150 mm × 2.0 mm i.d.) column maintained at 40 °C. Diclofenac sodium was used as an internal standard (IS) and EFV and IS were monitored at 247 nm and 275 nm, respectively. A simple and rapid sample preparation involved the addition of mobile phase to 100 μl of plasma to precipitate plasma proteins followed by direct injection of 10 μl of supernatant onto the column. The procedures were validated according to international standards with good reproducibility and linear response (r = 0.9990). The intra- and inter-day accuracies were between 12.3 and 17.7% at the LLOQ and between −5.8 and 9.1% for the QC samples. The intra- and inter-day precision of EFV determinations were 5.1 or less and 7.2% RSD or less, respectively across the entire QC concentration range. Mean recovery based on high, medium and low quality control standards ranged between 92.7 and 94.1% with %RSD values better than 3%. Plasma samples were evaluated for short-term (ambient temperature for 6 h) and long-term (−10 ± 2 °C for 60 days) storage conditions and were found to be stable. The method described is cost-effective and has the necessary accuracy and precision for the rapid quantitative determination of EFV in human plasma.
- Full Text:
Rapid UPLC - MS/MS method for the determination of ketoprofen in human dermal microdialysis samples
- Tettey-Amlalo, Ralph N O, Kanfer, Isadore
- Authors: Tettey-Amlalo, Ralph N O , Kanfer, Isadore
- Date: 2009
- Language: English
- Type: text , Article
- Identifier: vital:6444 , http://hdl.handle.net/10962/d1006631
- Description: Dermal microdialysis (DMD) is a technique capable of determining the percutaneous penetration of drugs from topical formulations intended for local and/or regional activity. Typically, the concentrations of drug collected in dialysates are very low, generally in the ng/ml or even pg/ml range. An additional challenge is the very low volume of sample collected at each collection time and which can range from 1 to 30 μl only. Hence the objective was to develop and validate a rapid, accurate, precise, reproducible and highly sensitive LC–MS/MS method for the quantitative analysis of ketoprofen (KET) in dialystes following application of a topical gel product to the skin of human subjects. UPLC–MS/MS was used and KET was separated on an Acquity™ UPLC BEH C18 column (100 mm × 2.1 mm i.d., 1.7 μm) and analysed in negative-ion (NI) electrospray ionisation (ESI) mode. The mobile phase (MP) consisted of acetonitrile:methanol:water (60:20:20, v/v/v) under isocratic conditions at a flow rate of 0.3 ml/min. Samples were extracted using ethyl acetate with ibuprofen (IBU) as internal standard (IS) and the organic solvent was then evaporated to dryness and the residue re-constituted in methanol. 5 μl samples were injected and analysis was performed at ambient temperature 22 ± 0.5 °C. KET and IBU eluted at 1.07 and 1.49 min, respectively. KET and IBU responses were optimised at the transitions 253.00 > 209.00 and 205.00 > 161.00, respectively. Calibration curves were linear over the range 0.5–500 ng/ml with correlation coefficients > 0.999. The accuracy and precision of the method were found to be between 99.97% and 104.67% (R.S.D. < 2%) and the mean recovery of KET from normal saline was 88.03 ± 0.3% (R.S.D. < 2.20%). The LLOQ and LOD values were found to be 0.5 and 0.1 ng/ml respectively whereas the ULOD was set at 500 ng/ml. The method was successfully applied to determine the bioavailability of KET following application of topical KET gel, Fastum® gel, to the skin of human volunteers.
- Full Text:
- Authors: Tettey-Amlalo, Ralph N O , Kanfer, Isadore
- Date: 2009
- Language: English
- Type: text , Article
- Identifier: vital:6444 , http://hdl.handle.net/10962/d1006631
- Description: Dermal microdialysis (DMD) is a technique capable of determining the percutaneous penetration of drugs from topical formulations intended for local and/or regional activity. Typically, the concentrations of drug collected in dialysates are very low, generally in the ng/ml or even pg/ml range. An additional challenge is the very low volume of sample collected at each collection time and which can range from 1 to 30 μl only. Hence the objective was to develop and validate a rapid, accurate, precise, reproducible and highly sensitive LC–MS/MS method for the quantitative analysis of ketoprofen (KET) in dialystes following application of a topical gel product to the skin of human subjects. UPLC–MS/MS was used and KET was separated on an Acquity™ UPLC BEH C18 column (100 mm × 2.1 mm i.d., 1.7 μm) and analysed in negative-ion (NI) electrospray ionisation (ESI) mode. The mobile phase (MP) consisted of acetonitrile:methanol:water (60:20:20, v/v/v) under isocratic conditions at a flow rate of 0.3 ml/min. Samples were extracted using ethyl acetate with ibuprofen (IBU) as internal standard (IS) and the organic solvent was then evaporated to dryness and the residue re-constituted in methanol. 5 μl samples were injected and analysis was performed at ambient temperature 22 ± 0.5 °C. KET and IBU eluted at 1.07 and 1.49 min, respectively. KET and IBU responses were optimised at the transitions 253.00 > 209.00 and 205.00 > 161.00, respectively. Calibration curves were linear over the range 0.5–500 ng/ml with correlation coefficients > 0.999. The accuracy and precision of the method were found to be between 99.97% and 104.67% (R.S.D. < 2%) and the mean recovery of KET from normal saline was 88.03 ± 0.3% (R.S.D. < 2.20%). The LLOQ and LOD values were found to be 0.5 and 0.1 ng/ml respectively whereas the ULOD was set at 500 ng/ml. The method was successfully applied to determine the bioavailability of KET following application of topical KET gel, Fastum® gel, to the skin of human volunteers.
- Full Text:
Recent progress in electrochemical oxidation of saccharides at gold and copper electrodes in alkaline solutions
- Authors: Torto, Nelson
- Date: 2009
- Language: English
- Type: Article
- Identifier: vital:6599 , http://hdl.handle.net/10962/d1004348
- Description: This article reviews the progress made in the past 10 years, on electrochemical oxidation of saccharides in alkaline media for gold and copper electrodes. The mechanism and processes associated with the electrochemical oxidation of saccharides at native and surface coated electrodes continues to be of great interest. Despite the effort and various mechanisms proposed, still the need for an electrochemically active material that understands the complexity associated with saccharides continues to increase as their detection poses a challenge for bioanalytical chemistry and liquid chromatography.
- Full Text:
- Authors: Torto, Nelson
- Date: 2009
- Language: English
- Type: Article
- Identifier: vital:6599 , http://hdl.handle.net/10962/d1004348
- Description: This article reviews the progress made in the past 10 years, on electrochemical oxidation of saccharides in alkaline media for gold and copper electrodes. The mechanism and processes associated with the electrochemical oxidation of saccharides at native and surface coated electrodes continues to be of great interest. Despite the effort and various mechanisms proposed, still the need for an electrochemically active material that understands the complexity associated with saccharides continues to increase as their detection poses a challenge for bioanalytical chemistry and liquid chromatography.
- Full Text:
Recontextualising issues in the 'NISTCOL" environmental education curriculum module for Primary Diploma by Distance Learning in Zambia
- Authors: Moose, John
- Date: 2009
- Subjects: Environmental education -- Zambia
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1722 , http://hdl.handle.net/10962/d1003605
- Description: This study investigated the recontextualising of the NISTCOL environmental education curriculum module for the Primary Teachers’ Diploma by Distance Learning (PTDDL) in Zambia. It focused on three case sites, the NISTCOL EE curriculum module for PTDDL and the University of Zambia, the National In-Service Teacher’s College (NISTCOL) and four basic schools: chalimbana, Bimbe, Chongwe and Silver Rest. The study looked at teacher professional development in environmental education in Zambia. The aims and goals were to investigate the NISTCOL EE curriculum and identifying issues that were associated with lecturers in the recontextualising process and further examine what issues were associated with curriculum recontextualisation in Zambian schools. For the conceptual constructs, the research drew on Bernstein’s (1990) framework of pedagogic discourse, it traced how the pedagogic discourse was de-located from the field of production and re-located into the pedagogic practice of each stated case above. It further examined the continuities, discontinuities and changes in the official environmental discourse as it was recontextualised. The study particularly focused on Bernstein’s conceptual constructs of selective appropriation and ideological transformation. In each case the Bernstein framework is used to analyze the process to identify recontextualising issues influencing pedagogical practice in the Zambian schools and college. The study revealed that each case under focus was unique but through examining the environmental discourse and pedagogical discourse in each case recontextalising issues were identified. Each case was influenced by different factors, such as lack of policy synergy, lack of EE vision at national level in Zambia, experience and knowledge, ideologies and emphasis, and depth of engagement. These factors provided me with a deeper insight into the curriculum recontextualising processes in Zambian schools.
- Full Text:
- Authors: Moose, John
- Date: 2009
- Subjects: Environmental education -- Zambia
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1722 , http://hdl.handle.net/10962/d1003605
- Description: This study investigated the recontextualising of the NISTCOL environmental education curriculum module for the Primary Teachers’ Diploma by Distance Learning (PTDDL) in Zambia. It focused on three case sites, the NISTCOL EE curriculum module for PTDDL and the University of Zambia, the National In-Service Teacher’s College (NISTCOL) and four basic schools: chalimbana, Bimbe, Chongwe and Silver Rest. The study looked at teacher professional development in environmental education in Zambia. The aims and goals were to investigate the NISTCOL EE curriculum and identifying issues that were associated with lecturers in the recontextualising process and further examine what issues were associated with curriculum recontextualisation in Zambian schools. For the conceptual constructs, the research drew on Bernstein’s (1990) framework of pedagogic discourse, it traced how the pedagogic discourse was de-located from the field of production and re-located into the pedagogic practice of each stated case above. It further examined the continuities, discontinuities and changes in the official environmental discourse as it was recontextualised. The study particularly focused on Bernstein’s conceptual constructs of selective appropriation and ideological transformation. In each case the Bernstein framework is used to analyze the process to identify recontextualising issues influencing pedagogical practice in the Zambian schools and college. The study revealed that each case under focus was unique but through examining the environmental discourse and pedagogical discourse in each case recontextalising issues were identified. Each case was influenced by different factors, such as lack of policy synergy, lack of EE vision at national level in Zambia, experience and knowledge, ideologies and emphasis, and depth of engagement. These factors provided me with a deeper insight into the curriculum recontextualising processes in Zambian schools.
- Full Text:
Redress : debates informing exhibitions and acquisitions in selected South African public art galleries (1990-1994)
- Authors: Cook, Shashi Chailey
- Date: 2009
- Subjects: South African National Gallery Exhibitions Durban Art Gallery Exhibitions Joahnnesburg Art Gallery Exhibitions Art -- South Africa -- Exhibitions Art, South African Exhibitions South Africa History Exhibitions
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2399 , http://hdl.handle.net/10962/d1002195
- Description: This thesis centres on the debates informing the progress of three public art galleries in South Africa between 1990 and 1994. This was a period of great change in the country, spanning from the unbanning of left-wing political parties and Nelson Mandela’s release from prison, to the first democratic elections which resulted in his inauguration as President of South Africa. The study focuses specifically on the Johannesburg Art Gallery, the South African National Gallery, and the Durban Art Gallery, delineating the events and exhibitions held, the programmes initiated, and the artists represented by these galleries during this post-apartheid/pre-democracy phase of the country’s history. The debates relevant to these galleries linked to those prevalent in the arts, museology, and politics at the time. Many contemporary South African artists called attention to apartheid oppression and human rights abuses during the 1980s. After 1990, with these pressures alleviating, there was a stage of uncertainty as to the role, responsibility, and focus of visual art in a post-‘struggle’ context, however there was also an unprecedented upswing in interest and investment in it. On a practical level, the administration of the arts was being re-evaluated and contested by both independent and politically-aligned arts groups. Public art museums and sponsored art competitions and exhibitions made increasing efforts to be ‘representative’ of South Africans of all races, cultures, creeds, sexes and genders. The many conferences, committees, and conventions created during this transitional era focused on the creation of policies that would assist in nation-building; historical and cultural redress and regeneration; and the education and representation of previously disadvantaged groups. This coincided with a revolution in museological discourses internationally, from the theorization of a museum as a place of commemoration and conservation, to a forum for discussion and revision between both academic and non-academic communities. With the sharing of the process of constructing history and knowledge, came the challenging dynamics involved in the representation of identity and history. In all of these groups - the arts, museology, and South African politics - the predominant issue seemed to be a negotiation between the bid to open up control to more parties, and the reluctance of some parties to relinquish control. While the emphasis is on significant changes that were implemented in the transitional period, the study locates the changes at the Johannesburg Art Gallery, the South African National Gallery and the Durban Art Gallery within their historical, geographical, and socio-political context. Various artists working in these locations during this era are also discussed, as the changes in their status, and the progressions in their subject matter, materials, and concerns are interesting to examine more nuanced definitions of the ‘political’, probing the politics of identity, sexuality, gender, race, geography, and belief systems. Some artists also focused specifically on post-apartheid preoccupations with territory, trauma, conflict, memory and freedom. This kind of artwork was assiduously acquired during the early ‘90s by public art galleries, whose exhibitions and collecting focus and policies were undergoing considerable revision and redress. This thesis examines these changes in light of their socio-political contexts, as well as in light of shifting national and international imperatives and conceptions of museums and museum practice.
- Full Text:
- Authors: Cook, Shashi Chailey
- Date: 2009
- Subjects: South African National Gallery Exhibitions Durban Art Gallery Exhibitions Joahnnesburg Art Gallery Exhibitions Art -- South Africa -- Exhibitions Art, South African Exhibitions South Africa History Exhibitions
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2399 , http://hdl.handle.net/10962/d1002195
- Description: This thesis centres on the debates informing the progress of three public art galleries in South Africa between 1990 and 1994. This was a period of great change in the country, spanning from the unbanning of left-wing political parties and Nelson Mandela’s release from prison, to the first democratic elections which resulted in his inauguration as President of South Africa. The study focuses specifically on the Johannesburg Art Gallery, the South African National Gallery, and the Durban Art Gallery, delineating the events and exhibitions held, the programmes initiated, and the artists represented by these galleries during this post-apartheid/pre-democracy phase of the country’s history. The debates relevant to these galleries linked to those prevalent in the arts, museology, and politics at the time. Many contemporary South African artists called attention to apartheid oppression and human rights abuses during the 1980s. After 1990, with these pressures alleviating, there was a stage of uncertainty as to the role, responsibility, and focus of visual art in a post-‘struggle’ context, however there was also an unprecedented upswing in interest and investment in it. On a practical level, the administration of the arts was being re-evaluated and contested by both independent and politically-aligned arts groups. Public art museums and sponsored art competitions and exhibitions made increasing efforts to be ‘representative’ of South Africans of all races, cultures, creeds, sexes and genders. The many conferences, committees, and conventions created during this transitional era focused on the creation of policies that would assist in nation-building; historical and cultural redress and regeneration; and the education and representation of previously disadvantaged groups. This coincided with a revolution in museological discourses internationally, from the theorization of a museum as a place of commemoration and conservation, to a forum for discussion and revision between both academic and non-academic communities. With the sharing of the process of constructing history and knowledge, came the challenging dynamics involved in the representation of identity and history. In all of these groups - the arts, museology, and South African politics - the predominant issue seemed to be a negotiation between the bid to open up control to more parties, and the reluctance of some parties to relinquish control. While the emphasis is on significant changes that were implemented in the transitional period, the study locates the changes at the Johannesburg Art Gallery, the South African National Gallery and the Durban Art Gallery within their historical, geographical, and socio-political context. Various artists working in these locations during this era are also discussed, as the changes in their status, and the progressions in their subject matter, materials, and concerns are interesting to examine more nuanced definitions of the ‘political’, probing the politics of identity, sexuality, gender, race, geography, and belief systems. Some artists also focused specifically on post-apartheid preoccupations with territory, trauma, conflict, memory and freedom. This kind of artwork was assiduously acquired during the early ‘90s by public art galleries, whose exhibitions and collecting focus and policies were undergoing considerable revision and redress. This thesis examines these changes in light of their socio-political contexts, as well as in light of shifting national and international imperatives and conceptions of museums and museum practice.
- Full Text:
Reflections on the sine causa requirements and the condictiones in South African law
- Authors: Glover, Graham B
- Date: 2009
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/70668 , vital:29687 , https://hdl.handle.net/10520/EJC54716
- Description: The condictiones present interesting and difficult challenges of classification for the modern South African jurist. Their casuistic development has made them difficult to work with, and for a long time it was argued that a single general enrichment action should take their place. But the Supreme Court of Appeal in McCarthy Retail Ltd v Shortdistance Carriers CC 2001 3 SA 482 (SCA) chose not to follow this path, and instead preferred the idea that a general enrichment action should be subsidiary to the traditional actions. This has breathed new life into debates on the future of the condictiones. This article first examines the current range of application of the condictiones. It then proceeds to show how the courts in the first decade of the 21st century seem to have eschewed the technicalities inherent in pleading the condictiones, and have preferred rather to resolve cases by applying the general requirements of enrichment liability, in contrast to the injunction in McCarthy. The difficulty with this approach is the lack of substance that is given to the sine causa requirement. The article proceeds to examine the various theories about how we in South Africa should understand and give content to the sine causa requirement. This provides a springboard for considering the possible futures of the condictiones. Broadly, it seems that two options are possible: either to collapse the condictiones into one action to deal with cases of enrichment by transfer; or largely to retain the status quo. The most recent decisions of the Supreme Court of Appeal indicate that the latter, more conservative option is likely to be chosen in the short-term. If this approach is to be adopted, it will require a further review of how each of the constituent condictiones fulfils a particular function. A revisionist view of the condictio indebiti is postulated by way of example.
- Full Text: false
- Authors: Glover, Graham B
- Date: 2009
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/70668 , vital:29687 , https://hdl.handle.net/10520/EJC54716
- Description: The condictiones present interesting and difficult challenges of classification for the modern South African jurist. Their casuistic development has made them difficult to work with, and for a long time it was argued that a single general enrichment action should take their place. But the Supreme Court of Appeal in McCarthy Retail Ltd v Shortdistance Carriers CC 2001 3 SA 482 (SCA) chose not to follow this path, and instead preferred the idea that a general enrichment action should be subsidiary to the traditional actions. This has breathed new life into debates on the future of the condictiones. This article first examines the current range of application of the condictiones. It then proceeds to show how the courts in the first decade of the 21st century seem to have eschewed the technicalities inherent in pleading the condictiones, and have preferred rather to resolve cases by applying the general requirements of enrichment liability, in contrast to the injunction in McCarthy. The difficulty with this approach is the lack of substance that is given to the sine causa requirement. The article proceeds to examine the various theories about how we in South Africa should understand and give content to the sine causa requirement. This provides a springboard for considering the possible futures of the condictiones. Broadly, it seems that two options are possible: either to collapse the condictiones into one action to deal with cases of enrichment by transfer; or largely to retain the status quo. The most recent decisions of the Supreme Court of Appeal indicate that the latter, more conservative option is likely to be chosen in the short-term. If this approach is to be adopted, it will require a further review of how each of the constituent condictiones fulfils a particular function. A revisionist view of the condictio indebiti is postulated by way of example.
- Full Text: false
Reform in defence of sovereignty: South Africa in the UN Security Council, 2007–2008
- Authors: Bischoff, Paul, 1954-
- Date: 2009
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/161431 , vital:40626 , https://0-doi.org.wam.seals.ac.za/10.1177/000203970904400205
- Description: After 1994, South Africa became the sine qua non of an internationalist state, willing to promote cooperation amongst a plurality of actors, believing common interests to be more important than their differences. This raised the hopes of constitutionalists, and those who believed in the expansion of a liberal democratic peace. South Africa has acted out two seemingly contradictory roles: those of a reformer and those of a conserver. By 2007–2008 she had shifted towards the latter, conservative-reformist position. Thus, South Africa's voting record at the General Assembly expressed her overriding concern to regionalise African issues and minimise the US and the West shaping political events. This brought her foreign policy into sharper relief. But while in some sense successful, it came at a price: a controversy about her surrendering her internationalism and principles on human rights for African unity and traditional sovereignty. But it also marked the arrival of South Africa in the world of international Realpolitik.
- Full Text:
- Authors: Bischoff, Paul, 1954-
- Date: 2009
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/161431 , vital:40626 , https://0-doi.org.wam.seals.ac.za/10.1177/000203970904400205
- Description: After 1994, South Africa became the sine qua non of an internationalist state, willing to promote cooperation amongst a plurality of actors, believing common interests to be more important than their differences. This raised the hopes of constitutionalists, and those who believed in the expansion of a liberal democratic peace. South Africa has acted out two seemingly contradictory roles: those of a reformer and those of a conserver. By 2007–2008 she had shifted towards the latter, conservative-reformist position. Thus, South Africa's voting record at the General Assembly expressed her overriding concern to regionalise African issues and minimise the US and the West shaping political events. This brought her foreign policy into sharper relief. But while in some sense successful, it came at a price: a controversy about her surrendering her internationalism and principles on human rights for African unity and traditional sovereignty. But it also marked the arrival of South Africa in the world of international Realpolitik.
- Full Text:
Regulating franchise operations in South Africa : a study of the existing legal framework with suggestions for reform.
- Authors: Woker, Tanya Ann
- Date: 2009
- Subjects: Franchise Association of Southern Africa , Franchises (Retail trade) -- Law and legislation -- South Africa , South Africa. Dept. of Trade and Industry , Common law -- South Africa , Commercial law -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3721 , http://hdl.handle.net/10962/d1015719
- Description: This thesis analyses the existing legal framework that applies to franchising in South Africa today. The study begins with an examination of the history and nature of the franchise contract, focusing particularly on the nature of the franchise relationship. This study is undertaken in order to substantiate the argument that franchising is a unique method of doing business. There is a need therefore to recognise that the franchise contract is a special contract in its own right, just like contracts of sale, lease, insurance and suretyship. The study then goes on to examine the problems which are experienced in the sector, as well as the law which must provide solutions to these problems. The research will show that in a modern commercial world the existing legal framework, especially the common law, cannot adequately deal with many of these problems. The complex relationship between franchising and competition law is also explored. A common thread that emerges from franchise disputes is the lack of protection afforded to the interests of franchisees. Franchisees tend to be at the mercy of economically stronger franchisors, hence the belief that there is a need for a stronger regulatory framework. The study then shifts to proposals for reform. In 2000 the Department of Trade and Industry (DTI) established the Franchise Steering Committee in conjunction with the Franchise Association of South Africa (FASA) to review the regulatory environment. This Committee drafted franchise legislation which aimed to bring the regulation of the sector under the control of the DTI. This legislation has not been implemented and the DTI has changed its strategy. Instead of dealing with franchising independently, franchising will fall within the scope of consumer protection legislation. Both the consumer protection legislation and the legislation proposed by the Franchise Steering Committee are thoroughly examined and explained. Shortcomings in the proposals are highlighted and an alternative approach is recommended. It is proposed that franchise-specific legislation should be introduced but that this legislation should establish a system of co-regulation between the government and the franchise sector.
- Full Text:
- Authors: Woker, Tanya Ann
- Date: 2009
- Subjects: Franchise Association of Southern Africa , Franchises (Retail trade) -- Law and legislation -- South Africa , South Africa. Dept. of Trade and Industry , Common law -- South Africa , Commercial law -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:3721 , http://hdl.handle.net/10962/d1015719
- Description: This thesis analyses the existing legal framework that applies to franchising in South Africa today. The study begins with an examination of the history and nature of the franchise contract, focusing particularly on the nature of the franchise relationship. This study is undertaken in order to substantiate the argument that franchising is a unique method of doing business. There is a need therefore to recognise that the franchise contract is a special contract in its own right, just like contracts of sale, lease, insurance and suretyship. The study then goes on to examine the problems which are experienced in the sector, as well as the law which must provide solutions to these problems. The research will show that in a modern commercial world the existing legal framework, especially the common law, cannot adequately deal with many of these problems. The complex relationship between franchising and competition law is also explored. A common thread that emerges from franchise disputes is the lack of protection afforded to the interests of franchisees. Franchisees tend to be at the mercy of economically stronger franchisors, hence the belief that there is a need for a stronger regulatory framework. The study then shifts to proposals for reform. In 2000 the Department of Trade and Industry (DTI) established the Franchise Steering Committee in conjunction with the Franchise Association of South Africa (FASA) to review the regulatory environment. This Committee drafted franchise legislation which aimed to bring the regulation of the sector under the control of the DTI. This legislation has not been implemented and the DTI has changed its strategy. Instead of dealing with franchising independently, franchising will fall within the scope of consumer protection legislation. Both the consumer protection legislation and the legislation proposed by the Franchise Steering Committee are thoroughly examined and explained. Shortcomings in the proposals are highlighted and an alternative approach is recommended. It is proposed that franchise-specific legislation should be introduced but that this legislation should establish a system of co-regulation between the government and the franchise sector.
- Full Text: