The fitting of statistical distributions to wind data in coastal areas of South Africa
- Authors: Moodley, Kirshnee
- Date: 2012
- Subjects: Winds -- South Africa -- Port Elizabeth -- Speed -- Measurement , Wind power -- South Africa -- Port Elizabeth , Winds -- South Africa -- Port Elizabeth -- Measurement -- Mathematical models
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10579
- Description: Coastal South African cities like Port Elizabeth are said to have a strong potential for wind energy. This study aims to model wind data in order to be able assess the power potential belonging to a given site. The main challenge in modelling wind direction data is that it is categorized as circular data and therefore requires special techniques for handling that are different from usual statistical samples. Statistical tools such as descriptive measures and distribution fitting, were re-invented for directional data by researchers in this field. The von Mises distribution is a predominant distribution in circular statistics and is commonly used to describe wind directions. In this study, the circular principles described by previous researchers were developed by using the statistical software, Mathematica. Graphical methods to present the wind data were developed to give an overview of the behaviour of the winds in any given area. Data collected at Coega, an area near Port Elizabeth, South Africa, was used to illustrate the models which were established in this study. Circular distributions were fit to the directional data in order to make appropriate conclusions about the prevailing wind directions in this area.
- Full Text:
- Date Issued: 2012
- Authors: Moodley, Kirshnee
- Date: 2012
- Subjects: Winds -- South Africa -- Port Elizabeth -- Speed -- Measurement , Wind power -- South Africa -- Port Elizabeth , Winds -- South Africa -- Port Elizabeth -- Measurement -- Mathematical models
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10579
- Description: Coastal South African cities like Port Elizabeth are said to have a strong potential for wind energy. This study aims to model wind data in order to be able assess the power potential belonging to a given site. The main challenge in modelling wind direction data is that it is categorized as circular data and therefore requires special techniques for handling that are different from usual statistical samples. Statistical tools such as descriptive measures and distribution fitting, were re-invented for directional data by researchers in this field. The von Mises distribution is a predominant distribution in circular statistics and is commonly used to describe wind directions. In this study, the circular principles described by previous researchers were developed by using the statistical software, Mathematica. Graphical methods to present the wind data were developed to give an overview of the behaviour of the winds in any given area. Data collected at Coega, an area near Port Elizabeth, South Africa, was used to illustrate the models which were established in this study. Circular distributions were fit to the directional data in order to make appropriate conclusions about the prevailing wind directions in this area.
- Full Text:
- Date Issued: 2012
The ichthyofauna of the Wilderness Lakes System, Western Cape, with particular emphasis on alien fish species and their establishment success
- Authors: Olds, Alexis Amy
- Date: 2012
- Subjects: Introduced fishes -- South Africa -- Wilderness , Estuarine fishes -- South Africa -- Wilderness , Mozambique tilapia -- South Africa -- Wilderness , Western mosquitofish -- South Africa -- Wilderness , Carp -- South Africa -- Wilderness , Largemouth bass -- South Africa -- Wilderness , Estuarine fishes -- Effect of exotic animals on -- South Africa -- Wilderness , Fish communities -- South Africa -- Wilderness
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5288 , http://hdl.handle.net/10962/d1005132 , Introduced fishes -- South Africa -- Wilderness , Estuarine fishes -- South Africa -- Wilderness , Mozambique tilapia -- South Africa -- Wilderness , Western mosquitofish -- South Africa -- Wilderness , Carp -- South Africa -- Wilderness , Largemouth bass -- South Africa -- Wilderness , Estuarine fishes -- Effect of exotic animals on -- South Africa -- Wilderness , Fish communities -- South Africa -- Wilderness
- Description: Freshwater fish species have been introduced into freshwater systems around the world, primarily for aquaculture, ornamental fish trade and sport fishing. Their introduction into estuarine systems is uncommon however, instances do occur and their establishment success and impacts on these estuarine systems is not well documented. The extent of invasion by four freshwater fishes in a RAMSAR listed estuarine system, the Wilderness Lakes, Western Cape was investigated. This thesis determined the relative abundance and distribution of alien fishes in relation to the native fish biota, their establishment success in the system, what factors inhibited their establishment and whether the introduction of alien fishes negatively impacted the native fish biota. The distribution and abundance of fishes were assessed primarily using fyke nets, seine nets and gill nets in each of the lakes, interconnecting channels and the Touw Estuary. The fish fauna was made up of euryhaline marine species comprising 46%, native estuarine species comprising 18%, catadromous species comprising 7% and freshwater alien species comprising 29% of the total biomass sampled. A total of 26 species were sampled in the system, three of which were considered alien; Oreochromis mossambicus, Gambusia affinis and Cyprinus carpio, and Micropterus salmoides were not sampled but confirmed in the system. Establishment success was determined by evidence of: a sustainable breeding population, a wide distribution, abundant in the sampling area, and all size classes of fish sampled. Gambusia affinis and Oreochromis mossambicus have been recorded in the system for a minimum of 13 and 26 years respectively. They were widely distributed and highly abundant and are established in the system. Micropterus salmoides was first recorded in the system in 1985 but abundances have remained low with fish appearing to be limited to Island Lake and Langvlei. Reproduction appeared to be limited by higher salinity and these factors indicated that this was a casual species which relies on repeated introductions for population maintenance. Cyprinus carpio spawned successfully in 2010 and was widely distributed but abundances were still low with a total of 15 fish being sampled throughout the system, and was thus in the establishing phase. As these are considered freshwater alien species, the physico-chemical parameters in the estuarine environment inhibiting the establishment success of the alien fishes were investigated. Gambusia affinis and O. mossambicus were not limited by the physical environment, and while O. mossambicus cannot tolerate temperatures below 11⁰C, temperatures only dropped below its tolerance for a total of two days between February 2010 and February 2011. Cyprinus carpio and M. salmoides were restricted by salinity in Rondevlei and Langvlei but could tolerate salinity in Island Lake and the Touw Estuary during closed mouth phases. While adults appeared to tolerate the salinity in the system, egg and larval development could be affected thus reducing the viability of the population. The abundance of alien fishes did not negatively impact the abundance of native fish species. The interactions between the native and alien fish biota tended towards biotic acceptance where, as alien abundance increased so did native fish abundance. The impacts of the alien fishes on the native fish biota were assessed by comparing the fish community from a study completed in 1985 to the findings of this study. From these two studies there were no apparent negative impacts on the native fish biota and the fish community composition would most likely be structured by estuarine mouth opening events.
- Full Text:
- Date Issued: 2012
- Authors: Olds, Alexis Amy
- Date: 2012
- Subjects: Introduced fishes -- South Africa -- Wilderness , Estuarine fishes -- South Africa -- Wilderness , Mozambique tilapia -- South Africa -- Wilderness , Western mosquitofish -- South Africa -- Wilderness , Carp -- South Africa -- Wilderness , Largemouth bass -- South Africa -- Wilderness , Estuarine fishes -- Effect of exotic animals on -- South Africa -- Wilderness , Fish communities -- South Africa -- Wilderness
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5288 , http://hdl.handle.net/10962/d1005132 , Introduced fishes -- South Africa -- Wilderness , Estuarine fishes -- South Africa -- Wilderness , Mozambique tilapia -- South Africa -- Wilderness , Western mosquitofish -- South Africa -- Wilderness , Carp -- South Africa -- Wilderness , Largemouth bass -- South Africa -- Wilderness , Estuarine fishes -- Effect of exotic animals on -- South Africa -- Wilderness , Fish communities -- South Africa -- Wilderness
- Description: Freshwater fish species have been introduced into freshwater systems around the world, primarily for aquaculture, ornamental fish trade and sport fishing. Their introduction into estuarine systems is uncommon however, instances do occur and their establishment success and impacts on these estuarine systems is not well documented. The extent of invasion by four freshwater fishes in a RAMSAR listed estuarine system, the Wilderness Lakes, Western Cape was investigated. This thesis determined the relative abundance and distribution of alien fishes in relation to the native fish biota, their establishment success in the system, what factors inhibited their establishment and whether the introduction of alien fishes negatively impacted the native fish biota. The distribution and abundance of fishes were assessed primarily using fyke nets, seine nets and gill nets in each of the lakes, interconnecting channels and the Touw Estuary. The fish fauna was made up of euryhaline marine species comprising 46%, native estuarine species comprising 18%, catadromous species comprising 7% and freshwater alien species comprising 29% of the total biomass sampled. A total of 26 species were sampled in the system, three of which were considered alien; Oreochromis mossambicus, Gambusia affinis and Cyprinus carpio, and Micropterus salmoides were not sampled but confirmed in the system. Establishment success was determined by evidence of: a sustainable breeding population, a wide distribution, abundant in the sampling area, and all size classes of fish sampled. Gambusia affinis and Oreochromis mossambicus have been recorded in the system for a minimum of 13 and 26 years respectively. They were widely distributed and highly abundant and are established in the system. Micropterus salmoides was first recorded in the system in 1985 but abundances have remained low with fish appearing to be limited to Island Lake and Langvlei. Reproduction appeared to be limited by higher salinity and these factors indicated that this was a casual species which relies on repeated introductions for population maintenance. Cyprinus carpio spawned successfully in 2010 and was widely distributed but abundances were still low with a total of 15 fish being sampled throughout the system, and was thus in the establishing phase. As these are considered freshwater alien species, the physico-chemical parameters in the estuarine environment inhibiting the establishment success of the alien fishes were investigated. Gambusia affinis and O. mossambicus were not limited by the physical environment, and while O. mossambicus cannot tolerate temperatures below 11⁰C, temperatures only dropped below its tolerance for a total of two days between February 2010 and February 2011. Cyprinus carpio and M. salmoides were restricted by salinity in Rondevlei and Langvlei but could tolerate salinity in Island Lake and the Touw Estuary during closed mouth phases. While adults appeared to tolerate the salinity in the system, egg and larval development could be affected thus reducing the viability of the population. The abundance of alien fishes did not negatively impact the abundance of native fish species. The interactions between the native and alien fish biota tended towards biotic acceptance where, as alien abundance increased so did native fish abundance. The impacts of the alien fishes on the native fish biota were assessed by comparing the fish community from a study completed in 1985 to the findings of this study. From these two studies there were no apparent negative impacts on the native fish biota and the fish community composition would most likely be structured by estuarine mouth opening events.
- Full Text:
- Date Issued: 2012
The impact of clothing and protective gear on biophysical, physiological, perceptual and performance responses of rugby players during a simulated rugby protocol
- Authors: Cannon, Michael-John
- Date: 2012
- Subjects: Protective clothing -- Rugby football -- Health aspects -- Research , Rugby football injuries -- Prevention -- Research , Sports injuries -- Prevention -- Research
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5124 , http://hdl.handle.net/10962/d1005202 , Protective clothing -- Rugby football -- Health aspects -- Research , Rugby football injuries -- Prevention -- Research , Sports injuries -- Prevention -- Research
- Description: Background: Clothing and protective gear worn during intermittent exercise has shown to increase physiological and perceptual responses, and negatively impact performance capacities, due to increased heat strain, suggested to hasten the onset of fatigue. However, the mechanisms of fatigue experienced in rugby remain unclear. Objectives: The aim of this study was establish whether clothing and protective gear worn during a simulated rugby protocol impacts players‘ biophysical, physiological, perceptual and performance responses. Methods: 15 registered university and school first XV rugby players with a mean (± SD) age of 20.9 years (± 1.9) volunteered to participate in the study. Testing was performed in a controlled laboratory setting, with temperatures having to be within the range of 16º C-22º C. The mean (± SD) ambient temperature was 17.6º C (± 1.6) for the control condition and 17.3º C (1.5) for the experimental condition. The mean (± SD) relative humidity was 65.2 % (± 9.5) for the control condition and 66.3 % (± 10.0) for the experimental condition. Player‘s performed two protocols of 80-minutes; a control condition (minimal clothing and protective gear) and an experimental condition (full clothing and protective gear). Physiological, perceptual and performance responses were measured at set intervals during the protocol, while biophysical responses were measured pre-, at half-time and post-protocol during a 3-minute EMG treadmill protocol. Results: Muscle activity significantly (p< 0.05) increased with increasing running speeds. There were no significant (p> 0.05) differences for muscle activity between conditions, except for the semitendinosus muscle, which was significantly (p< 0.05) higher during the control condition while running at high speeds. Players‘ heart rates, core temperatures and perceptual responses were significantly (p< 0.05) higher during the experimental condition, compared to the control condition. Performance responses were significantly (p< 0.05) lower during the experimental condition. Conclusion: The main driver of physiological and perceptual responses was the exercise itself. However, the additional clothing and protective gear exacerbated the responses, particularly towards the end stages of the protocol. This negatively impacted players‘ performance. Muscle activity appeared to be unaffected by increased body temperatures. However, core temperatures never reached critically high levels during either condition.
- Full Text:
- Date Issued: 2012
- Authors: Cannon, Michael-John
- Date: 2012
- Subjects: Protective clothing -- Rugby football -- Health aspects -- Research , Rugby football injuries -- Prevention -- Research , Sports injuries -- Prevention -- Research
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5124 , http://hdl.handle.net/10962/d1005202 , Protective clothing -- Rugby football -- Health aspects -- Research , Rugby football injuries -- Prevention -- Research , Sports injuries -- Prevention -- Research
- Description: Background: Clothing and protective gear worn during intermittent exercise has shown to increase physiological and perceptual responses, and negatively impact performance capacities, due to increased heat strain, suggested to hasten the onset of fatigue. However, the mechanisms of fatigue experienced in rugby remain unclear. Objectives: The aim of this study was establish whether clothing and protective gear worn during a simulated rugby protocol impacts players‘ biophysical, physiological, perceptual and performance responses. Methods: 15 registered university and school first XV rugby players with a mean (± SD) age of 20.9 years (± 1.9) volunteered to participate in the study. Testing was performed in a controlled laboratory setting, with temperatures having to be within the range of 16º C-22º C. The mean (± SD) ambient temperature was 17.6º C (± 1.6) for the control condition and 17.3º C (1.5) for the experimental condition. The mean (± SD) relative humidity was 65.2 % (± 9.5) for the control condition and 66.3 % (± 10.0) for the experimental condition. Player‘s performed two protocols of 80-minutes; a control condition (minimal clothing and protective gear) and an experimental condition (full clothing and protective gear). Physiological, perceptual and performance responses were measured at set intervals during the protocol, while biophysical responses were measured pre-, at half-time and post-protocol during a 3-minute EMG treadmill protocol. Results: Muscle activity significantly (p< 0.05) increased with increasing running speeds. There were no significant (p> 0.05) differences for muscle activity between conditions, except for the semitendinosus muscle, which was significantly (p< 0.05) higher during the control condition while running at high speeds. Players‘ heart rates, core temperatures and perceptual responses were significantly (p< 0.05) higher during the experimental condition, compared to the control condition. Performance responses were significantly (p< 0.05) lower during the experimental condition. Conclusion: The main driver of physiological and perceptual responses was the exercise itself. However, the additional clothing and protective gear exacerbated the responses, particularly towards the end stages of the protocol. This negatively impacted players‘ performance. Muscle activity appeared to be unaffected by increased body temperatures. However, core temperatures never reached critically high levels during either condition.
- Full Text:
- Date Issued: 2012
The influence of nutrients on surf-zone phytoplankton
- Authors: Wolmarans, Karien
- Date: 2012
- Subjects: Phytoplankton , Algal blooms , Diatoms
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10608 , http://hdl.handle.net/10948/d1008080 , Phytoplankton , Algal blooms , Diatoms
- Description: The variability in surf-zone phytoplankton community composition together with variability in nutrient concentrations was studied at seven beaches along the coast near Port Elizabeth, South Africa over a one-year sampling period. The nutrient requirements of selected diatoms and dinoflagellates that co-occur at these beaches were studied. The highest nutrient concentrations were recorded at Brighton beach, with phosphate concentration being substantially higher than standards set out for both South African and European waters. Nitrate, ammonium and silicate concentrations were consistent (low variability) at all beaches. The species composition of beaches without surf diatoms was also not variable (except for King’s Beach – an artificial beach). Maitland beach was the only beach that could be identified as a surf diatom beach due to the presence of Anaulus australis. The surf diatom Anaulus australis was found to be a superior competitor compared to all other species tested: the other surf diatom Asterionellopsis glacialis, and the dinoflagellates Gonyaulax spinifera, Prorocentrum micans, and Scrippsiella trochoidea. Gonyaulax spinifera could possibly outcompete Anaulus australis in systems should nitrate become limiting.
- Full Text:
- Date Issued: 2012
- Authors: Wolmarans, Karien
- Date: 2012
- Subjects: Phytoplankton , Algal blooms , Diatoms
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10608 , http://hdl.handle.net/10948/d1008080 , Phytoplankton , Algal blooms , Diatoms
- Description: The variability in surf-zone phytoplankton community composition together with variability in nutrient concentrations was studied at seven beaches along the coast near Port Elizabeth, South Africa over a one-year sampling period. The nutrient requirements of selected diatoms and dinoflagellates that co-occur at these beaches were studied. The highest nutrient concentrations were recorded at Brighton beach, with phosphate concentration being substantially higher than standards set out for both South African and European waters. Nitrate, ammonium and silicate concentrations were consistent (low variability) at all beaches. The species composition of beaches without surf diatoms was also not variable (except for King’s Beach – an artificial beach). Maitland beach was the only beach that could be identified as a surf diatom beach due to the presence of Anaulus australis. The surf diatom Anaulus australis was found to be a superior competitor compared to all other species tested: the other surf diatom Asterionellopsis glacialis, and the dinoflagellates Gonyaulax spinifera, Prorocentrum micans, and Scrippsiella trochoidea. Gonyaulax spinifera could possibly outcompete Anaulus australis in systems should nitrate become limiting.
- Full Text:
- Date Issued: 2012
The influence of project management service provision on role-players within the South African construction industry
- Authors: Hefer, Andre le Roux
- Date: 2012
- Subjects: Project management -- South Africa , Construction industry -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:9663 , http://hdl.handle.net/10948/d1008295 , Project management -- South Africa , Construction industry -- South Africa
- Description: The legitimate existence of the Project Management Profession in the South African construction industry needs to relate to positive project influence on the industry roleplayers. This study assessed the perceived lack of recognition and acceptance of Project Management as a stand-alone profession relating to: · Appointments of Project Managers being questioned due to a perceived lack of influence and impact on project success. · Project Managers not being recognised as an integral part of the industry. · Project Management not being perceived as having a unique and defined function. Interviews were held with 23 industry role-players made up of clients, contractors and consultants. The interviews solicited input on the role-players’ perception and experience related to the influence of Project Management over the past 10 years, not only on a list of success criteria relative to their own role in the industry, but also their perception of the influence on the other defined role-player groups. Included in the interview questionnaire was also a range of general questions to refine the feedback and further test the hypotheses. The data were interpreted and analysed by comparing the feedback of the respondents as a combination and separately as groups. The results of the study indicate that: · Project Management could be seen as a legitimate part of the industry; · The industry role-players perceive Project Management as making an impact and having a growing influence on the industry; A specific but broad set of skills are required by Project Managers; · The Project Management function cannot be fulfilled by other consultants, but there are project related criteria which should be considered before making a final judgment. These criteria relate to project size and complexity; and · Project Management is currently perceived to be more related to a specific person’s skill than to a specific profession. The study’s aim was to influence and inform the views of industry role-players on the appointment of a Project Manager in the construction industry.
- Full Text:
- Date Issued: 2012
- Authors: Hefer, Andre le Roux
- Date: 2012
- Subjects: Project management -- South Africa , Construction industry -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:9663 , http://hdl.handle.net/10948/d1008295 , Project management -- South Africa , Construction industry -- South Africa
- Description: The legitimate existence of the Project Management Profession in the South African construction industry needs to relate to positive project influence on the industry roleplayers. This study assessed the perceived lack of recognition and acceptance of Project Management as a stand-alone profession relating to: · Appointments of Project Managers being questioned due to a perceived lack of influence and impact on project success. · Project Managers not being recognised as an integral part of the industry. · Project Management not being perceived as having a unique and defined function. Interviews were held with 23 industry role-players made up of clients, contractors and consultants. The interviews solicited input on the role-players’ perception and experience related to the influence of Project Management over the past 10 years, not only on a list of success criteria relative to their own role in the industry, but also their perception of the influence on the other defined role-player groups. Included in the interview questionnaire was also a range of general questions to refine the feedback and further test the hypotheses. The data were interpreted and analysed by comparing the feedback of the respondents as a combination and separately as groups. The results of the study indicate that: · Project Management could be seen as a legitimate part of the industry; · The industry role-players perceive Project Management as making an impact and having a growing influence on the industry; A specific but broad set of skills are required by Project Managers; · The Project Management function cannot be fulfilled by other consultants, but there are project related criteria which should be considered before making a final judgment. These criteria relate to project size and complexity; and · Project Management is currently perceived to be more related to a specific person’s skill than to a specific profession. The study’s aim was to influence and inform the views of industry role-players on the appointment of a Project Manager in the construction industry.
- Full Text:
- Date Issued: 2012
The isolation, quantification and synthetic modification of antiplasmodial natural products from sargassum heterophyllum
- Authors: Munedzimwe, Tatenda Carol
- Date: 2012
- Subjects: Malaria -- Developing countries -- Prevention , Antimalarials
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3871 , http://hdl.handle.net/10962/d1018252
- Description: Malaria is one of the most deadly parasitic diseases known to man. Although the number of malaria cases reported each year is decreasing, this disease continues to pose health and economic problems mainly in developing countries. Significant progress has been made in the fight against this disease. This includes the discovery and development of potent antimalarial agents. However, the development of resistance to most of these potent antimalarials has made the development of new antiplasmodial agents of paramount importance. Several promising antiplasmodial agents have been found from the marine environment. Amongst these are the tetraprenylated toluquinols from the brown alga: Sargassum heterophyllum. These metabolites have been reported to exhibit a range of antiplasmodial activity; however, the mechanisms by which these compounds bring about their antiplasmodial activity and the pharmacophoric groups responsible for such activity are unknown. Two species of Sargassum algae were encountered during the course of this project. From the investigation of the geographical and seasonal variation of metabolites of S. heterophyllum and S. elegans we established that there were no significant intra and inter site variations amongst metabolite profiles of both species both within and between the sampled seasons. These results enabled us to establish that the collection of both species from three different sites on the eastern coast of South Africa namely; Kenton on Sea, Port Alfred and Noordhoek in autumn, winter or spring would qualitatively yield the same metabolites. A comparison of metabolite profiles of both species also revealed no qualitative differences between metabolites of S. heterophyllum and S. elegans. The quantities of selected prenylated metabolites extracted from S. heterophyllum using four different extraction techniques was also assessed using qNMR as the method of quantification. This led to the identification of optimal extraction techniques and conditions for the extraction of sargahydroquinoic acid (1.38), sargaquinoic aid (1.39) and sargachromenol (2.10) from S. heterophyllum. From this study, the extraction of algae by soxhlet extraction using EtOH as the extraction solvent led to the extraction of the highest quantities of sargahydroquinoic acid. The potential of other extraction techniques such as microwave assisted extraction, to yield high quantities of the selected metabolites were also identified. With gram quantities of sargahydroquinoic acid (1.38) in hand, this compound was modified by oxidation, reduction, acetylation, methylation and cyclization reactions to yield nine derivatives. The derivatives and four naturally occurring prenylated toluquinols were assessed for antiplasmodial and cytotoxic activity against the FCR-3 Gambian Chloroquine resistant strain of P. falciparum and the MDA-MB-231 breast carcinoma cell line respectively. Comparison of antiplasmodial data for all twelve compounds showed that the hydroquinone moeity of sargahydroquinoic acid (1.38) is important for antiplasmodial activity while esterification of the carboxylic acid group in 1.38 resulted in more potent antiplasmodial compounds. Of all twelve compounds, compound 5.2, the hydroquinone methyl ester of 1.38 was found to be the most potent antiplasmodial compound with an IC₅₀ value of 1.94 μM and a selectivity index of 22.68.
- Full Text:
- Date Issued: 2012
- Authors: Munedzimwe, Tatenda Carol
- Date: 2012
- Subjects: Malaria -- Developing countries -- Prevention , Antimalarials
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3871 , http://hdl.handle.net/10962/d1018252
- Description: Malaria is one of the most deadly parasitic diseases known to man. Although the number of malaria cases reported each year is decreasing, this disease continues to pose health and economic problems mainly in developing countries. Significant progress has been made in the fight against this disease. This includes the discovery and development of potent antimalarial agents. However, the development of resistance to most of these potent antimalarials has made the development of new antiplasmodial agents of paramount importance. Several promising antiplasmodial agents have been found from the marine environment. Amongst these are the tetraprenylated toluquinols from the brown alga: Sargassum heterophyllum. These metabolites have been reported to exhibit a range of antiplasmodial activity; however, the mechanisms by which these compounds bring about their antiplasmodial activity and the pharmacophoric groups responsible for such activity are unknown. Two species of Sargassum algae were encountered during the course of this project. From the investigation of the geographical and seasonal variation of metabolites of S. heterophyllum and S. elegans we established that there were no significant intra and inter site variations amongst metabolite profiles of both species both within and between the sampled seasons. These results enabled us to establish that the collection of both species from three different sites on the eastern coast of South Africa namely; Kenton on Sea, Port Alfred and Noordhoek in autumn, winter or spring would qualitatively yield the same metabolites. A comparison of metabolite profiles of both species also revealed no qualitative differences between metabolites of S. heterophyllum and S. elegans. The quantities of selected prenylated metabolites extracted from S. heterophyllum using four different extraction techniques was also assessed using qNMR as the method of quantification. This led to the identification of optimal extraction techniques and conditions for the extraction of sargahydroquinoic acid (1.38), sargaquinoic aid (1.39) and sargachromenol (2.10) from S. heterophyllum. From this study, the extraction of algae by soxhlet extraction using EtOH as the extraction solvent led to the extraction of the highest quantities of sargahydroquinoic acid. The potential of other extraction techniques such as microwave assisted extraction, to yield high quantities of the selected metabolites were also identified. With gram quantities of sargahydroquinoic acid (1.38) in hand, this compound was modified by oxidation, reduction, acetylation, methylation and cyclization reactions to yield nine derivatives. The derivatives and four naturally occurring prenylated toluquinols were assessed for antiplasmodial and cytotoxic activity against the FCR-3 Gambian Chloroquine resistant strain of P. falciparum and the MDA-MB-231 breast carcinoma cell line respectively. Comparison of antiplasmodial data for all twelve compounds showed that the hydroquinone moeity of sargahydroquinoic acid (1.38) is important for antiplasmodial activity while esterification of the carboxylic acid group in 1.38 resulted in more potent antiplasmodial compounds. Of all twelve compounds, compound 5.2, the hydroquinone methyl ester of 1.38 was found to be the most potent antiplasmodial compound with an IC₅₀ value of 1.94 μM and a selectivity index of 22.68.
- Full Text:
- Date Issued: 2012
The life history and fishery assessment of largespot pompano, Trachinotus botla, in northern KwaZulu-Natal, South Africa
- Authors: Parker, Denham
- Date: 2012
- Subjects: Trachinotus -- South Africa -- KwaZulu-Natal , Carangidae -- South Africa -- KwaZulu-Natal , Fishing -- South Africa -- KwaZulu-Natal , Fishery management -- South Africa -- KwaZulu-Natal , Fishing -- Economic aspects -- South Africa -- KwaZulu-Natal , Fish stock assessment -- South Africa -- KwaZulu-Natal , Trachinotus -- Growth -- South Africa -- KwaZulu-Natal , Trachinotus -- Breeding -- South Africa -- KwaZulu-Natal , Trachinotus -- Food -- South Africa -- KwaZulu-Natal , Host-parasite relationships -- South Africa -- KwaZulu-Natal
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5296 , http://hdl.handle.net/10962/d1005141 , Trachinotus -- South Africa -- KwaZulu-Natal , Carangidae -- South Africa -- KwaZulu-Natal , Fishing -- South Africa -- KwaZulu-Natal , Fishery management -- South Africa -- KwaZulu-Natal , Fishing -- Economic aspects -- South Africa -- KwaZulu-Natal , Fish stock assessment -- South Africa -- KwaZulu-Natal , Trachinotus -- Growth -- South Africa -- KwaZulu-Natal , Trachinotus -- Breeding -- South Africa -- KwaZulu-Natal , Trachinotus -- Food -- South Africa -- KwaZulu-Natal , Host-parasite relationships -- South Africa -- KwaZulu-Natal
- Description: Largespot pompano, Trachinotus botla, is a surf zone carangid with a cosmopolitan distribution in subtropical and tropical waters. Within South Africa, the species occurs along the KwaZulu-Natal coastline where it is a popular recreational fishing target. Recreational fishing in southern Africa has developed significantly in recent years, and is now regarded as an industry with huge economic potential. The long-term contribution of South African recreational fisheries to local economies is reliant upon sustainable exploitation through effective management. Trachinotus botla was found to grow rapidly with maximum observed age of six years. Otolith growth zone deposition was validated using edge analysis. Growth was similar between males and females until ~350 mm FL after which females continued to grow while growth in males slowed. The resulting overall sex ratio was slightly female-biased (1 male: 1.3 females). Trachinotus botla matures early with all fish considered to be mature at 290 mm FL, which corresponds to an age of three years. A protracted spawning season was observed ranging from November to February and there was evidence to suggest that T. botla is a serial spawner. Dietary analysis indicates that T. botla is an opportunistic predator with a catholic diet. The opportunistic utilization of “superabundant” prey items is a fundamental characteristic of the species feeding habits. An ontogenetic dietary shift was observed at approximately 300 mm FL that was linked to a shift in habitat preference. This thesis provided the first evidence that infection by the tongue-replacing isopod, Cymothoa borbonica, reduces the growth rate of wild host fish populations despite not affecting the diet, feeding habits and feeding frequency of their hosts. These results also highlighted the inadequacy of condition factor as a proxy for quantifying the effects of cymothoids on their hosts, and identified the need to incorporate host age when assessing the effects of parasite infection. Information on the life-cycle of C. borbonica, including estimates of the hypothesized “infectious” period and its longevity were obtained through analysis of parasite infection patterns as a function of host age and length. An assessment of the T. botla shore fishery of KwaZulu-Natal using historical catch data revealed that the fishery is stable. Productivity of the T. botla fishery increased towards the north of KwaZulu-Natal. Distinct seasonal variations in the T. botla fishery were also noted with catches peaking in summer months and lowest during winter. A per-recruit assessment revealed that the species is currently underexploited (SBR = 62% of pristine levels), and fishing mortality rate could be doubled before reaching the spawner biomass-per recruit target reference point of FSB₄₀. A combination of the life history characteristics of species, the nature of the recreational shore fishery together with the current management regulation of 5 fish person⁻¹ day⁻¹ has ensured the sustainable utilization of the T. botla resource in KwaZulu-Natal.
- Full Text:
- Date Issued: 2012
- Authors: Parker, Denham
- Date: 2012
- Subjects: Trachinotus -- South Africa -- KwaZulu-Natal , Carangidae -- South Africa -- KwaZulu-Natal , Fishing -- South Africa -- KwaZulu-Natal , Fishery management -- South Africa -- KwaZulu-Natal , Fishing -- Economic aspects -- South Africa -- KwaZulu-Natal , Fish stock assessment -- South Africa -- KwaZulu-Natal , Trachinotus -- Growth -- South Africa -- KwaZulu-Natal , Trachinotus -- Breeding -- South Africa -- KwaZulu-Natal , Trachinotus -- Food -- South Africa -- KwaZulu-Natal , Host-parasite relationships -- South Africa -- KwaZulu-Natal
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5296 , http://hdl.handle.net/10962/d1005141 , Trachinotus -- South Africa -- KwaZulu-Natal , Carangidae -- South Africa -- KwaZulu-Natal , Fishing -- South Africa -- KwaZulu-Natal , Fishery management -- South Africa -- KwaZulu-Natal , Fishing -- Economic aspects -- South Africa -- KwaZulu-Natal , Fish stock assessment -- South Africa -- KwaZulu-Natal , Trachinotus -- Growth -- South Africa -- KwaZulu-Natal , Trachinotus -- Breeding -- South Africa -- KwaZulu-Natal , Trachinotus -- Food -- South Africa -- KwaZulu-Natal , Host-parasite relationships -- South Africa -- KwaZulu-Natal
- Description: Largespot pompano, Trachinotus botla, is a surf zone carangid with a cosmopolitan distribution in subtropical and tropical waters. Within South Africa, the species occurs along the KwaZulu-Natal coastline where it is a popular recreational fishing target. Recreational fishing in southern Africa has developed significantly in recent years, and is now regarded as an industry with huge economic potential. The long-term contribution of South African recreational fisheries to local economies is reliant upon sustainable exploitation through effective management. Trachinotus botla was found to grow rapidly with maximum observed age of six years. Otolith growth zone deposition was validated using edge analysis. Growth was similar between males and females until ~350 mm FL after which females continued to grow while growth in males slowed. The resulting overall sex ratio was slightly female-biased (1 male: 1.3 females). Trachinotus botla matures early with all fish considered to be mature at 290 mm FL, which corresponds to an age of three years. A protracted spawning season was observed ranging from November to February and there was evidence to suggest that T. botla is a serial spawner. Dietary analysis indicates that T. botla is an opportunistic predator with a catholic diet. The opportunistic utilization of “superabundant” prey items is a fundamental characteristic of the species feeding habits. An ontogenetic dietary shift was observed at approximately 300 mm FL that was linked to a shift in habitat preference. This thesis provided the first evidence that infection by the tongue-replacing isopod, Cymothoa borbonica, reduces the growth rate of wild host fish populations despite not affecting the diet, feeding habits and feeding frequency of their hosts. These results also highlighted the inadequacy of condition factor as a proxy for quantifying the effects of cymothoids on their hosts, and identified the need to incorporate host age when assessing the effects of parasite infection. Information on the life-cycle of C. borbonica, including estimates of the hypothesized “infectious” period and its longevity were obtained through analysis of parasite infection patterns as a function of host age and length. An assessment of the T. botla shore fishery of KwaZulu-Natal using historical catch data revealed that the fishery is stable. Productivity of the T. botla fishery increased towards the north of KwaZulu-Natal. Distinct seasonal variations in the T. botla fishery were also noted with catches peaking in summer months and lowest during winter. A per-recruit assessment revealed that the species is currently underexploited (SBR = 62% of pristine levels), and fishing mortality rate could be doubled before reaching the spawner biomass-per recruit target reference point of FSB₄₀. A combination of the life history characteristics of species, the nature of the recreational shore fishery together with the current management regulation of 5 fish person⁻¹ day⁻¹ has ensured the sustainable utilization of the T. botla resource in KwaZulu-Natal.
- Full Text:
- Date Issued: 2012
The medicinal chemistry of Cyclo (D-PHE-4I-PRO) and Cyclo (L-PHE-4I-PRO)
- Authors: Qhola, Lipolelo
- Date: 2012
- Subjects: Cyclic peptides , Pharmaceutical chemistry , Peptide drugs
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10152 , http://hdl.handle.net/10948/d1011619 , Cyclic peptides , Pharmaceutical chemistry , Peptide drugs
- Description: Cyclic dipeptides have been widely used as pharmaceutical agents due to their favourable properties and the fact that they are more stable and membrane permeable than their linear analogues. These characteristics make cyclic dipeptides attractive to protein-based drug developers (Martins & Carvalho, 2007). In this research study, the method of Milne et al. (1992) was used to synthesize the protected linear dipeptide esters. This was followed by boiling the unprotected, linear dipeptide esters under reflux in an oil bath (Sec-butanol: toluene (4:1)). This method gave good yields and pure cyclic dipeptides. Scanning electron microscopy, thermal analysis, X-ray powder diffraction and differential scanning calorimetry were used for evaluation of the physiochemical properties of the cyclic dipeptides. High-performance liquid chromatography and thin layer chromatography were used to determine the purity of the cyclic dipeptides. The structures of the cyclic dipeptides were elucidated using infrared spectroscopy, mass spectrometry, nuclear magnetic resonance spectroscopy and molecular modeling and computational chemistry. The aim of the study was to determine the possible therapeutic activity of cyclo(D-Phe-4I-Pro) and cyclo(L-Phe-4I-Pro) with regard to antimicrobial, anticancer, antidiabetes and haematological effects. Both cyclic dipeptides showed a significant growth inhibition of Gram-positive, Gram-negative and fungal microorganisms in the antimicrobial study. Anticancer studies showed that both cyclic dipeptides caused growth inhibition of the MCF-7, HT-29 and HeLa cancer cell lines. Both cyclic dipeptides showed no antidiabetic activity. Haematological studies revealed that both cyclic dipeptides caused a significant effect on the clotting time and platelet aggregation. They caused an increase in clotting time and also inhibited platelet aggregation.
- Full Text:
- Date Issued: 2012
- Authors: Qhola, Lipolelo
- Date: 2012
- Subjects: Cyclic peptides , Pharmaceutical chemistry , Peptide drugs
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10152 , http://hdl.handle.net/10948/d1011619 , Cyclic peptides , Pharmaceutical chemistry , Peptide drugs
- Description: Cyclic dipeptides have been widely used as pharmaceutical agents due to their favourable properties and the fact that they are more stable and membrane permeable than their linear analogues. These characteristics make cyclic dipeptides attractive to protein-based drug developers (Martins & Carvalho, 2007). In this research study, the method of Milne et al. (1992) was used to synthesize the protected linear dipeptide esters. This was followed by boiling the unprotected, linear dipeptide esters under reflux in an oil bath (Sec-butanol: toluene (4:1)). This method gave good yields and pure cyclic dipeptides. Scanning electron microscopy, thermal analysis, X-ray powder diffraction and differential scanning calorimetry were used for evaluation of the physiochemical properties of the cyclic dipeptides. High-performance liquid chromatography and thin layer chromatography were used to determine the purity of the cyclic dipeptides. The structures of the cyclic dipeptides were elucidated using infrared spectroscopy, mass spectrometry, nuclear magnetic resonance spectroscopy and molecular modeling and computational chemistry. The aim of the study was to determine the possible therapeutic activity of cyclo(D-Phe-4I-Pro) and cyclo(L-Phe-4I-Pro) with regard to antimicrobial, anticancer, antidiabetes and haematological effects. Both cyclic dipeptides showed a significant growth inhibition of Gram-positive, Gram-negative and fungal microorganisms in the antimicrobial study. Anticancer studies showed that both cyclic dipeptides caused growth inhibition of the MCF-7, HT-29 and HeLa cancer cell lines. Both cyclic dipeptides showed no antidiabetic activity. Haematological studies revealed that both cyclic dipeptides caused a significant effect on the clotting time and platelet aggregation. They caused an increase in clotting time and also inhibited platelet aggregation.
- Full Text:
- Date Issued: 2012
The medicinal chemistry of cyclo(Phe-4CI-Pro) and Cyclo(D-Phe-4CI-Pro)
- Authors: Milne, Marnus
- Date: 2012
- Subjects: Cyclic peptides , Pharmaceutical chemistry , Peptide drugs
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10157 , http://hdl.handle.net/10948/d1011848 , Cyclic peptides , Pharmaceutical chemistry , Peptide drugs
- Description: Cyclic dipeptides have limited conformational freedom due to their diketopiperazine backbone and their small size. They are relatively simple to synthesise, making them ideal subjects for investigation into their biological effects. Cyclic dipeptides have also been known for their multitude of biological activities, including antimicrobial, anticancer and haematological properties. In this study the cyclic dipeptides, cyclo(Phe-4Cl-Pro) and cyclo(D-Phe-4Cl-Pro), were synthesised from their corresponding linear precursors using a modified phenol-induced cyclisation procedure. The phenol induced cyclisation procedure resulted in good yields and purity of the cyclic dipeptides. Quantitative analysis and evaluation of the physiochemical properties of the cyclic dipeptides was achieved using high-performance liquid chromatography, scanning electron microscopy, thermal analysis and X-ray powder diffraction. Structural elucidation of the cyclic dipeptides was done by means of infrared spectroscopy, mass spectroscopy, nuclear magnetic resonance spectroscopy and molecular modelling. The study‟s aim was to determine the biological activity of cyclo(Phe-4Cl-Pro) and cyclo(D-Phe-4Cl-Pro) with respect to their anticancer, antimicrobial, haematological and ant-diabetic studies. Anticancer studies revealed that cyclo(Phe-4Cl-Pro) and cyclo(D-Phe-4Cl-Pro) inhibited the growth of HeLa (cervical cancer), HT-29 (colon cancer) and MCF-7 (breast cancer) cancer cell lines. Both cyclic dipeptides also inhibited the growth of certain selected Gram-positive, Gram-negative and fungal microorganisms in the antimicrobial study. Although the inhibition of growth in the anticancer and antimicrobial studies was statistically significant, the clinical relevance is questionable, since the inhibition produced by both cyclic dipeptides was very limited compared to other pre-existing anticancer and antimicrobial agents. Both cyclic dipeptides caused a significant shortening of the APTT and PT clotting times and an increase in the fibrin and D-Dimer formation. Cyclo(D-Phe-4Cl-Pro) at a screening concentration of 12.5 mM and 3.125 mM, showed significant anti-platelet activity. Both cyclic dipeptides failed to produce any inhibition of the α-Glucosidase enzyme and very limited inhibition of the α-Amylase enzyme.
- Full Text:
- Date Issued: 2012
- Authors: Milne, Marnus
- Date: 2012
- Subjects: Cyclic peptides , Pharmaceutical chemistry , Peptide drugs
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10157 , http://hdl.handle.net/10948/d1011848 , Cyclic peptides , Pharmaceutical chemistry , Peptide drugs
- Description: Cyclic dipeptides have limited conformational freedom due to their diketopiperazine backbone and their small size. They are relatively simple to synthesise, making them ideal subjects for investigation into their biological effects. Cyclic dipeptides have also been known for their multitude of biological activities, including antimicrobial, anticancer and haematological properties. In this study the cyclic dipeptides, cyclo(Phe-4Cl-Pro) and cyclo(D-Phe-4Cl-Pro), were synthesised from their corresponding linear precursors using a modified phenol-induced cyclisation procedure. The phenol induced cyclisation procedure resulted in good yields and purity of the cyclic dipeptides. Quantitative analysis and evaluation of the physiochemical properties of the cyclic dipeptides was achieved using high-performance liquid chromatography, scanning electron microscopy, thermal analysis and X-ray powder diffraction. Structural elucidation of the cyclic dipeptides was done by means of infrared spectroscopy, mass spectroscopy, nuclear magnetic resonance spectroscopy and molecular modelling. The study‟s aim was to determine the biological activity of cyclo(Phe-4Cl-Pro) and cyclo(D-Phe-4Cl-Pro) with respect to their anticancer, antimicrobial, haematological and ant-diabetic studies. Anticancer studies revealed that cyclo(Phe-4Cl-Pro) and cyclo(D-Phe-4Cl-Pro) inhibited the growth of HeLa (cervical cancer), HT-29 (colon cancer) and MCF-7 (breast cancer) cancer cell lines. Both cyclic dipeptides also inhibited the growth of certain selected Gram-positive, Gram-negative and fungal microorganisms in the antimicrobial study. Although the inhibition of growth in the anticancer and antimicrobial studies was statistically significant, the clinical relevance is questionable, since the inhibition produced by both cyclic dipeptides was very limited compared to other pre-existing anticancer and antimicrobial agents. Both cyclic dipeptides caused a significant shortening of the APTT and PT clotting times and an increase in the fibrin and D-Dimer formation. Cyclo(D-Phe-4Cl-Pro) at a screening concentration of 12.5 mM and 3.125 mM, showed significant anti-platelet activity. Both cyclic dipeptides failed to produce any inhibition of the α-Glucosidase enzyme and very limited inhibition of the α-Amylase enzyme.
- Full Text:
- Date Issued: 2012
The molecular analysis of the interation surface between sCD23 and the B2-integrins, CD11b & CD11c
- Authors: Pereira, Melanie Claire
- Date: 2012
- Subjects: CD23 antigen , Immune response -- Regulation
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10337 , http://hdl.handle.net/10948/d1014734
- Description: Both CD23 and the β2 integrins (also known as CD11/CD18) have very important immunological functions, especially during the allergic response where the binding of CD23 to β2 integrins contributes to various types of signalling in monocytes which can result in drastic sensitivities experienced by some allergic individuals. CD23, also known as the low affinity receptor for immunoglobulin E or (FcεRII), is a type II transmembrane glycoprotein which is synthesized by haematopoietic cells and has biological activity in both membrane-bound and freely soluble forms. It acts via a number of receptors, including the β2 integrins. β2 integrins are specifically found on leukocytes and they play important roles in cell–cell or cell–matrix adhesion via their ability to bind multiple ligands. These molecules occur as heterodimers consisting of an alpha (α) and beta (β) subunit. The α-subunits of β2 integrins contain an approximately 200-amino-acid inserted domain or I-domain which is implicated in ligand binding function. There are four different types of β2 integrins, namely CD11a, CD11b, CD11c and CD11d, all dimers with the common beta subunit, CD18. CD23 and CD11/18 are natural ligands of each other; however the interaction site for CD23 is unknown. It is postulated that the integrin recognizes a tripeptide motif in a small disulfide-bonded loop at the N-terminus of the lectin head region of CD23, which is focussed around Arg172, Lys173 and Cys174 (RKC). This study thus focused on the interaction between the I-domain of CD11 (b and c) and a recombinant 25kDa construct of sCD23. In order to understand the characteristics of ligand binding between the relevant proteins of interest, alanine substitutions on the RKC motif of CD23 were made via site-directed mutagenesis. Consequently, a recombinant form of the I-domain of CD11 (b and c) as well as a wild type (containing the RKC motif) and mutant form (containing an AAC motif) of sCD23 were expressed and purified. The CD11 recombinant proteins were purified via affinity chromatography and the CD23 recombinant proteins via gel filtration chromatography. In addition, synthetic (CD23 derived) peptides, one containing the RKC sequence and the other the AAC sequence, were designed and custom synthesized. The synthetic peptides as well as the recombinant CD23 proteins were then analyzed for their interaction with the CD11 I-domain via ELISA. Subsequent ELISA analyses showed that the native sCD23 and the RKC peptide were able to bind to the integrin α I-domain whereas the mutant sCD23 and the corresponding synthetic AAC peptide failed to bind. This interaction was also analysed via flow cytometry using differentiated U937 cells, yielding similar results. ELISA analyses for the sCD23-CD11b I-domain interaction showed a Kd of 0.36 ± 0.14 μM whereas the RKC-CD11b I-domain interaction yielded a Kd of 1.75 ± 0.58 μM. Similarly, the sCD23-CD11c I-domain interaction yielded a Kd of 0.39 ± 0.09 μM and 1.53 ± 0.72 μM for the RKC-CD11c I-domain interaction. Peptide inhibitory analysis, analysed via ELISA and flow cytometry, reinforced the fact that the RKC motif on sCD23 is a prerequisite for ligand binding of the CD11b/c I-domain.
- Full Text:
- Date Issued: 2012
- Authors: Pereira, Melanie Claire
- Date: 2012
- Subjects: CD23 antigen , Immune response -- Regulation
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10337 , http://hdl.handle.net/10948/d1014734
- Description: Both CD23 and the β2 integrins (also known as CD11/CD18) have very important immunological functions, especially during the allergic response where the binding of CD23 to β2 integrins contributes to various types of signalling in monocytes which can result in drastic sensitivities experienced by some allergic individuals. CD23, also known as the low affinity receptor for immunoglobulin E or (FcεRII), is a type II transmembrane glycoprotein which is synthesized by haematopoietic cells and has biological activity in both membrane-bound and freely soluble forms. It acts via a number of receptors, including the β2 integrins. β2 integrins are specifically found on leukocytes and they play important roles in cell–cell or cell–matrix adhesion via their ability to bind multiple ligands. These molecules occur as heterodimers consisting of an alpha (α) and beta (β) subunit. The α-subunits of β2 integrins contain an approximately 200-amino-acid inserted domain or I-domain which is implicated in ligand binding function. There are four different types of β2 integrins, namely CD11a, CD11b, CD11c and CD11d, all dimers with the common beta subunit, CD18. CD23 and CD11/18 are natural ligands of each other; however the interaction site for CD23 is unknown. It is postulated that the integrin recognizes a tripeptide motif in a small disulfide-bonded loop at the N-terminus of the lectin head region of CD23, which is focussed around Arg172, Lys173 and Cys174 (RKC). This study thus focused on the interaction between the I-domain of CD11 (b and c) and a recombinant 25kDa construct of sCD23. In order to understand the characteristics of ligand binding between the relevant proteins of interest, alanine substitutions on the RKC motif of CD23 were made via site-directed mutagenesis. Consequently, a recombinant form of the I-domain of CD11 (b and c) as well as a wild type (containing the RKC motif) and mutant form (containing an AAC motif) of sCD23 were expressed and purified. The CD11 recombinant proteins were purified via affinity chromatography and the CD23 recombinant proteins via gel filtration chromatography. In addition, synthetic (CD23 derived) peptides, one containing the RKC sequence and the other the AAC sequence, were designed and custom synthesized. The synthetic peptides as well as the recombinant CD23 proteins were then analyzed for their interaction with the CD11 I-domain via ELISA. Subsequent ELISA analyses showed that the native sCD23 and the RKC peptide were able to bind to the integrin α I-domain whereas the mutant sCD23 and the corresponding synthetic AAC peptide failed to bind. This interaction was also analysed via flow cytometry using differentiated U937 cells, yielding similar results. ELISA analyses for the sCD23-CD11b I-domain interaction showed a Kd of 0.36 ± 0.14 μM whereas the RKC-CD11b I-domain interaction yielded a Kd of 1.75 ± 0.58 μM. Similarly, the sCD23-CD11c I-domain interaction yielded a Kd of 0.39 ± 0.09 μM and 1.53 ± 0.72 μM for the RKC-CD11c I-domain interaction. Peptide inhibitory analysis, analysed via ELISA and flow cytometry, reinforced the fact that the RKC motif on sCD23 is a prerequisite for ligand binding of the CD11b/c I-domain.
- Full Text:
- Date Issued: 2012
The performance of O R Tambo district municipality regarding water service delivery
- Authors: Xala, Zandile
- Date: 2012
- Subjects: Public administration|zSouth Africa , Local government , Government accountability
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:16141 , http://hdl.handle.net/10948/d1009318 , Public administration|zSouth Africa , Local government , Government accountability
- Description: Access to water services in South Africa still remains a dream for most people, especially those who live in remote areas. The poverty stricken communities of the former Transkei in the Eastern Cape are in need of water and sanitation services. The findings of the study reveal that most community members are dissatisfied with the water service delivery. During an interview with ward 6 residents who reside in an urban area of the OR Tambo District Municipality (ORTDM), they revealed that pipe bursting is one of the main causes why from time to time they do not have water. The other factor is when there is no electricity; the water system depends on the availability of electricity. The South African local government has been mandated to consult with its citizenry in respect of the processes relating to water service delivery. South Africa has developed a wide range of legislation that ensures community consultation on a continuous basis with regard to how water services should be delivered. It is the community‟s right to be consulted and to give input on issues that directly affect them. The researcher used a qualitative research method since it would provide the reader with more insight into how water services are delivered in the ORTDM. Both community members and municipal officials were interviewed. A number of findings have been made during the research. Some community members are of the view that constant community consultation could reduce service delivery protests. Political intervention needs to be made by the leadership with regard to service delivery and more resources need to be committed toward those processes. This study was conducted from April 2012 to November 2012 and was aimed at assessing the performance of OR Tambo District Municipality regarding water service delivery. According to OR Tambo DM (2011/12: 3), the communities through the consultation processes conducted throughout five (5) local municipalities made it clear that ensuring increased access to water and sanitation services is still the main priority.
- Full Text:
- Date Issued: 2012
- Authors: Xala, Zandile
- Date: 2012
- Subjects: Public administration|zSouth Africa , Local government , Government accountability
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:16141 , http://hdl.handle.net/10948/d1009318 , Public administration|zSouth Africa , Local government , Government accountability
- Description: Access to water services in South Africa still remains a dream for most people, especially those who live in remote areas. The poverty stricken communities of the former Transkei in the Eastern Cape are in need of water and sanitation services. The findings of the study reveal that most community members are dissatisfied with the water service delivery. During an interview with ward 6 residents who reside in an urban area of the OR Tambo District Municipality (ORTDM), they revealed that pipe bursting is one of the main causes why from time to time they do not have water. The other factor is when there is no electricity; the water system depends on the availability of electricity. The South African local government has been mandated to consult with its citizenry in respect of the processes relating to water service delivery. South Africa has developed a wide range of legislation that ensures community consultation on a continuous basis with regard to how water services should be delivered. It is the community‟s right to be consulted and to give input on issues that directly affect them. The researcher used a qualitative research method since it would provide the reader with more insight into how water services are delivered in the ORTDM. Both community members and municipal officials were interviewed. A number of findings have been made during the research. Some community members are of the view that constant community consultation could reduce service delivery protests. Political intervention needs to be made by the leadership with regard to service delivery and more resources need to be committed toward those processes. This study was conducted from April 2012 to November 2012 and was aimed at assessing the performance of OR Tambo District Municipality regarding water service delivery. According to OR Tambo DM (2011/12: 3), the communities through the consultation processes conducted throughout five (5) local municipalities made it clear that ensuring increased access to water and sanitation services is still the main priority.
- Full Text:
- Date Issued: 2012
The role of Hsp90/Hsp70 organising protein (Hop) in the Proliferation, Survival and Migration of Breast Cancer Cells.
- Authors: Willmer, Tarryn
- Date: 2012
- Subjects: Cancer -- Treatment , Heat shock proteins , Cancer cells , Breast -- Cancer
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4130 , http://hdl.handle.net/10962/d1015720
- Description: Hop (the Hsp90/Hsp70 organising protein) is a co-chaperone that acts as an adapter between the major molecular chaperones Hsp90 and Hsp70 during the cellular assembly of the Hsp90 complex. The Hsp90 complex regulates the stability and conformational maturation of a range of important cellular proteins, many of which are deregulated in cancer. In this study, we hypothesised that Hop knockdown inhibits proliferation and migration of cancer cells. We characterised the expression of Hop in cell models of different cancerous status, and provided evidence that Hop was upregulated in tumour cells compared to normal cell counterparts. Using an RNA interference approach, a 60-90% knockdown of Hop was achieved for up to 144 hours in the MDA-MB-231 and Hs578T breast cancer cell lines. Hop knockdown resulted in downregulation of the Hsp90 client proteins, Akt and Stat3, as well as a change in the expression of other Hsp90 co-chaperones, p23, Cdc37 and Aha1, while no change in the levels of Hsp90 or Hsp70 was observed. Silencing of Hop impaired cell proliferation in Hs578T cells but an increase in proliferation in MDA-MB-231, suggesting that the role of Hop in cancer cell proliferation was dependent on type of cancer cell. Hop knockdown in Hs578T and MDA-MB- 231 cells did not lead to any significant changes in the half maximal inhibitory concentrations (IC50) of selected small molecule inhibitors (paclitaxel, geldanamycin and novobiocin) in these cell lines after 72 hours. Hop knockdown cells were however, more sensitive than control cells to the Hsp90 inhibitors geldanamycin and novobiocin at earlier time points and in the presence of the drug transporter inhibitor, verapamil. Hop knockdown caused a decrease in cell migration as measured by the wound healing assay in both Hs578T and MDA-MB-231 cells. Hop was present in purified pseudopodia fractions of migrating cells, and immunofluorescence analysis showed that Hop colocalised with actin at the leading edges of pseudopodia, points of adhesion and at intercellular junctions of cells that have been stimulated to migrate with the chemokine stromal derived factor-1. Hop was able to bind to actin in vitro using actin cosedimentation assays, and silencing of Hop dramatically reduced the capacity of Hs578T cells to form pseudopodia. These results establish a correlation between Hop and actin dynamics, pseudopodia formation and migration in the context of Hop silencing, and collectively suggest that Hop plays a role in cancer cell migration. This study presents experimental evidence for a promising alternative to targeting Hsp90 and Hsp70 chaperones, a novel drug target in cancer therapy.
- Full Text:
- Date Issued: 2012
- Authors: Willmer, Tarryn
- Date: 2012
- Subjects: Cancer -- Treatment , Heat shock proteins , Cancer cells , Breast -- Cancer
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4130 , http://hdl.handle.net/10962/d1015720
- Description: Hop (the Hsp90/Hsp70 organising protein) is a co-chaperone that acts as an adapter between the major molecular chaperones Hsp90 and Hsp70 during the cellular assembly of the Hsp90 complex. The Hsp90 complex regulates the stability and conformational maturation of a range of important cellular proteins, many of which are deregulated in cancer. In this study, we hypothesised that Hop knockdown inhibits proliferation and migration of cancer cells. We characterised the expression of Hop in cell models of different cancerous status, and provided evidence that Hop was upregulated in tumour cells compared to normal cell counterparts. Using an RNA interference approach, a 60-90% knockdown of Hop was achieved for up to 144 hours in the MDA-MB-231 and Hs578T breast cancer cell lines. Hop knockdown resulted in downregulation of the Hsp90 client proteins, Akt and Stat3, as well as a change in the expression of other Hsp90 co-chaperones, p23, Cdc37 and Aha1, while no change in the levels of Hsp90 or Hsp70 was observed. Silencing of Hop impaired cell proliferation in Hs578T cells but an increase in proliferation in MDA-MB-231, suggesting that the role of Hop in cancer cell proliferation was dependent on type of cancer cell. Hop knockdown in Hs578T and MDA-MB- 231 cells did not lead to any significant changes in the half maximal inhibitory concentrations (IC50) of selected small molecule inhibitors (paclitaxel, geldanamycin and novobiocin) in these cell lines after 72 hours. Hop knockdown cells were however, more sensitive than control cells to the Hsp90 inhibitors geldanamycin and novobiocin at earlier time points and in the presence of the drug transporter inhibitor, verapamil. Hop knockdown caused a decrease in cell migration as measured by the wound healing assay in both Hs578T and MDA-MB-231 cells. Hop was present in purified pseudopodia fractions of migrating cells, and immunofluorescence analysis showed that Hop colocalised with actin at the leading edges of pseudopodia, points of adhesion and at intercellular junctions of cells that have been stimulated to migrate with the chemokine stromal derived factor-1. Hop was able to bind to actin in vitro using actin cosedimentation assays, and silencing of Hop dramatically reduced the capacity of Hs578T cells to form pseudopodia. These results establish a correlation between Hop and actin dynamics, pseudopodia formation and migration in the context of Hop silencing, and collectively suggest that Hop plays a role in cancer cell migration. This study presents experimental evidence for a promising alternative to targeting Hsp90 and Hsp70 chaperones, a novel drug target in cancer therapy.
- Full Text:
- Date Issued: 2012
The symmetry group of a model of hyperbolic plane geometry and some associated invariant optimal control problems
- Authors: Henninger, Helen Clare
- Date: 2012
- Subjects: Geometry , Symmetry groups , Symmetry (Mathematics)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5432 , http://hdl.handle.net/10962/d1018232
- Description: In this thesis we study left-invariant control offine systems on the symmetry group of a. model of hyperbolic plane geometry, the matrix Lie group SO(1, 2)₀. We determine that there are 10 distinct classes of such control systems and for typical elements of two of these classes we provide solutions of the left-invariant optimal wntrol problem with quauratic costs. Under the identification of the Lie allgebra .so(l, 2) with Minkowski spacetime R¹̕'², we construct a controllabilility criterion for all left-invariant control affine systems on 50(1. 2)₀ which in the inhomogeneous case depends only on the presence or absence of an element in the image of the system's trace in R¹̕ ²which is identifiable using the inner product. For the solutions of both the optimal control problems, we provide explicit expressions in terms of Jacobi elliptic functions for the solutions of the reduced extremal equations and determine the nonlinear stability of the equilibrium points.
- Full Text:
- Date Issued: 2012
- Authors: Henninger, Helen Clare
- Date: 2012
- Subjects: Geometry , Symmetry groups , Symmetry (Mathematics)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5432 , http://hdl.handle.net/10962/d1018232
- Description: In this thesis we study left-invariant control offine systems on the symmetry group of a. model of hyperbolic plane geometry, the matrix Lie group SO(1, 2)₀. We determine that there are 10 distinct classes of such control systems and for typical elements of two of these classes we provide solutions of the left-invariant optimal wntrol problem with quauratic costs. Under the identification of the Lie allgebra .so(l, 2) with Minkowski spacetime R¹̕'², we construct a controllabilility criterion for all left-invariant control affine systems on 50(1. 2)₀ which in the inhomogeneous case depends only on the presence or absence of an element in the image of the system's trace in R¹̕ ²which is identifiable using the inner product. For the solutions of both the optimal control problems, we provide explicit expressions in terms of Jacobi elliptic functions for the solutions of the reduced extremal equations and determine the nonlinear stability of the equilibrium points.
- Full Text:
- Date Issued: 2012
The synthesis and breast cancer inhibitory activity of cinnamic acid analogues based on the halogenated monoterpene pharmacophore
- Authors: Chiwakata, Maynard Tendai
- Date: 2012
- Subjects: Halocarbons , Cancer -- Treatment , Breast -- Cancer -- Treatment
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3866 , http://hdl.handle.net/10962/d1016129
- Description: Breast cancer is one of the leading causes of death, with mortality rate estimates of 465 000 deaths per annum. It is estimated that 1.3 million women are diagnosed with the disease each year especially in the developing countries. Current chemotherapy relies on the use of high doses of non-specific toxic agents that possess adverse side effects and compromise patient’s compliance and adherence to treatment. Paclitaxel, one of the common drugs used in breast cancer chemotherapy results in sensory and motor neuropathy, whilst hormonal therapy e.g. Herceptin causes severe cardiovascular, gastrointestinal and cutaneous side effects. There has been a demand in developing newer cancer agents that demonstrate selective cytoxicity with minimal effect on normal body tissue. Numerous studies have shown that marine organisms produce a wide range of halogenated compounds that possess cytotoxic properties, and hence can be a source of new drug hits or leads for cancer therapy. Halomon, a polyhalogenated monoterpene from Portieria hornemannii, displayed interesting activity against brain, renal and lung cancer tumours with selective/differential cytotoxicity. This inspired us to focus our project on halogenated monoterpenes isolated from the same Rhodophyta class as P. hornemannii but with particular attention to Plocamium species. Several metabolites have been isolated from P. cornutum, P. corallorhiza and P. suhrii that possess interesting cytotoxicities against a breast cancer cell line (MCF7) and an oesophageal cancer line (WHCO1). The aim of the project was therefore centred at isolating target compounds for preliminary structure-activity studies against a breast cancer cell line, and use this information to synthesize a series of analogues that are more stable than the natural products and yet as active using a fragment-based type approach to map out pharmacophoric elements. Five metabolites were isolated from P. cornutum and five from P. corallorhiza. Cell-based assays were conducted using an MTT assay kit against MCF7 and MDA-MB-231 breast cancer cell lines and (1E,3E,5S,6R)-1,5,6-trichloro-2-(dichloromethyl)-6-methylocta-1,3,7-triene, isolated from P. cornutum was the most active with IC50 values of 3.0 μM and 6.15 μM respectively. Introduction of a terminal aromatic ring to enhance stability, together with varying substituents (H, CH3, CF3, Br, CN, CHO, CHCl2) on position 7 of the molecule, gave rise to a series of cinnamate ester derivatives inspired by (1E,3E,5S,6R)-1,5,6-trichloro-2-(dichloromethyl)-6-methylocta-1,3,7-triene. The analogues were synthesized from their benzaldehyde precursors via Aldol condensation, esterification and Wittig reactions. Their carboxylic acid counterparts were synthesized by hydrolysis of the parent esters in an attempt to promote water solubilities of the analogues. Biological activity assays were then conducted with the cinnamate analogues against the MDA-MB-231 breast cancer cell line using an MTT assay kit. Ester derivatives with -CHO and -CHCl2 functionalities had IC50 values of 43.45 μM and 100.01 μM respectively whilst the other ester derivatives were inactive. It was concluded that either an aldehyde (-CHO) or gem-dichlorides (-CHCl2) is specifically required for cytotoxic activity to be observed. None of the carboxylic acids were active which could have been due to failure of the compounds to enter the breast cancer cells and reach the target site because of their polar nature. Compounds with -CHO and -CHCl2 functionalities were therefore selected for future SARs studies.
- Full Text:
- Date Issued: 2012
- Authors: Chiwakata, Maynard Tendai
- Date: 2012
- Subjects: Halocarbons , Cancer -- Treatment , Breast -- Cancer -- Treatment
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3866 , http://hdl.handle.net/10962/d1016129
- Description: Breast cancer is one of the leading causes of death, with mortality rate estimates of 465 000 deaths per annum. It is estimated that 1.3 million women are diagnosed with the disease each year especially in the developing countries. Current chemotherapy relies on the use of high doses of non-specific toxic agents that possess adverse side effects and compromise patient’s compliance and adherence to treatment. Paclitaxel, one of the common drugs used in breast cancer chemotherapy results in sensory and motor neuropathy, whilst hormonal therapy e.g. Herceptin causes severe cardiovascular, gastrointestinal and cutaneous side effects. There has been a demand in developing newer cancer agents that demonstrate selective cytoxicity with minimal effect on normal body tissue. Numerous studies have shown that marine organisms produce a wide range of halogenated compounds that possess cytotoxic properties, and hence can be a source of new drug hits or leads for cancer therapy. Halomon, a polyhalogenated monoterpene from Portieria hornemannii, displayed interesting activity against brain, renal and lung cancer tumours with selective/differential cytotoxicity. This inspired us to focus our project on halogenated monoterpenes isolated from the same Rhodophyta class as P. hornemannii but with particular attention to Plocamium species. Several metabolites have been isolated from P. cornutum, P. corallorhiza and P. suhrii that possess interesting cytotoxicities against a breast cancer cell line (MCF7) and an oesophageal cancer line (WHCO1). The aim of the project was therefore centred at isolating target compounds for preliminary structure-activity studies against a breast cancer cell line, and use this information to synthesize a series of analogues that are more stable than the natural products and yet as active using a fragment-based type approach to map out pharmacophoric elements. Five metabolites were isolated from P. cornutum and five from P. corallorhiza. Cell-based assays were conducted using an MTT assay kit against MCF7 and MDA-MB-231 breast cancer cell lines and (1E,3E,5S,6R)-1,5,6-trichloro-2-(dichloromethyl)-6-methylocta-1,3,7-triene, isolated from P. cornutum was the most active with IC50 values of 3.0 μM and 6.15 μM respectively. Introduction of a terminal aromatic ring to enhance stability, together with varying substituents (H, CH3, CF3, Br, CN, CHO, CHCl2) on position 7 of the molecule, gave rise to a series of cinnamate ester derivatives inspired by (1E,3E,5S,6R)-1,5,6-trichloro-2-(dichloromethyl)-6-methylocta-1,3,7-triene. The analogues were synthesized from their benzaldehyde precursors via Aldol condensation, esterification and Wittig reactions. Their carboxylic acid counterparts were synthesized by hydrolysis of the parent esters in an attempt to promote water solubilities of the analogues. Biological activity assays were then conducted with the cinnamate analogues against the MDA-MB-231 breast cancer cell line using an MTT assay kit. Ester derivatives with -CHO and -CHCl2 functionalities had IC50 values of 43.45 μM and 100.01 μM respectively whilst the other ester derivatives were inactive. It was concluded that either an aldehyde (-CHO) or gem-dichlorides (-CHCl2) is specifically required for cytotoxic activity to be observed. None of the carboxylic acids were active which could have been due to failure of the compounds to enter the breast cancer cells and reach the target site because of their polar nature. Compounds with -CHO and -CHCl2 functionalities were therefore selected for future SARs studies.
- Full Text:
- Date Issued: 2012
The synthesis of fructooligosaccharides by the fructofuranosidase FopAp from Aspergillus niger
- Pindura, Mitchell Kingsley Chido
- Authors: Pindura, Mitchell Kingsley Chido
- Date: 2012
- Subjects: Aspergillus niger , Oligosaccharides
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4158 , http://hdl.handle.net/10962/d1018267
- Description: Fructooligosaccharides (FOS) are short-chain fructans with a terminal glucose moiety and are found naturally in many plant species. Besides their wide use as an alternative sweetener in food and beverage industry, FOS have shown great potential as neutraceuticals against diabetes, colon cancer and bowel disease. The uses of FOS are dependent on the degree of polymerisation that they exhibit. β-fructofuranosidase (FFase) and fructosyltransferase (FTase) enzymes are capable of synthesing FOS from carbohydrate raw materials such as chicory and sugar beet. The aim of this study was to investigate the synthesis of FOS of a pre-defined chain length, from sucrose, by the enzyme FopAp; a β-fructofuranosidase from Aspergillus niger. ATCC 20611. The crude enzyme FopAp was successfully purified, with a yield of 78.20 %, by ammonium sulphate precipitation and anion exchange chromatography. Two protein fractions, named FA and FB were shown to exhibit FFase activity. SDS PAGE analysis revealed two proteins with molecular weights of 112 kDa and 78 kDa, which were identified as a FFase and a hydrolase. Temperature and pH optima of 20 ºC and 9, respectively, were observed for the transfructosylation activity in the FFase. The purified FFase exhibited a half life of 1.5 hrs under optimal conditions. Substrate kinetic studies indicated a high hydrolytic activity at low sucrose concentrations, with Vmax and Km of 1.25 μmol/ml/min and 3.28 mM, respectively. Analysis by response surface methodology identified temperature and pH to be significant factors for the production of kestose and nystose, at a 95 % level of confidence. These findings were confirmed by neural networks constructed to identify optimal conditions of FOS synthesis.FOS synthesis was found to be optimal between pH 6 and pH 9 at 25 ºC. The factor of reaction time was found to be insignificant within the selected experimental constraints, for both FOS species. The findings of this investigation are very important as the foundations of a commercially viable synthetic process for the production of FOS.
- Full Text:
- Date Issued: 2012
- Authors: Pindura, Mitchell Kingsley Chido
- Date: 2012
- Subjects: Aspergillus niger , Oligosaccharides
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4158 , http://hdl.handle.net/10962/d1018267
- Description: Fructooligosaccharides (FOS) are short-chain fructans with a terminal glucose moiety and are found naturally in many plant species. Besides their wide use as an alternative sweetener in food and beverage industry, FOS have shown great potential as neutraceuticals against diabetes, colon cancer and bowel disease. The uses of FOS are dependent on the degree of polymerisation that they exhibit. β-fructofuranosidase (FFase) and fructosyltransferase (FTase) enzymes are capable of synthesing FOS from carbohydrate raw materials such as chicory and sugar beet. The aim of this study was to investigate the synthesis of FOS of a pre-defined chain length, from sucrose, by the enzyme FopAp; a β-fructofuranosidase from Aspergillus niger. ATCC 20611. The crude enzyme FopAp was successfully purified, with a yield of 78.20 %, by ammonium sulphate precipitation and anion exchange chromatography. Two protein fractions, named FA and FB were shown to exhibit FFase activity. SDS PAGE analysis revealed two proteins with molecular weights of 112 kDa and 78 kDa, which were identified as a FFase and a hydrolase. Temperature and pH optima of 20 ºC and 9, respectively, were observed for the transfructosylation activity in the FFase. The purified FFase exhibited a half life of 1.5 hrs under optimal conditions. Substrate kinetic studies indicated a high hydrolytic activity at low sucrose concentrations, with Vmax and Km of 1.25 μmol/ml/min and 3.28 mM, respectively. Analysis by response surface methodology identified temperature and pH to be significant factors for the production of kestose and nystose, at a 95 % level of confidence. These findings were confirmed by neural networks constructed to identify optimal conditions of FOS synthesis.FOS synthesis was found to be optimal between pH 6 and pH 9 at 25 ºC. The factor of reaction time was found to be insignificant within the selected experimental constraints, for both FOS species. The findings of this investigation are very important as the foundations of a commercially viable synthetic process for the production of FOS.
- Full Text:
- Date Issued: 2012
The synthesis of α-alkoxy and α-aminostannanes as precursors to Novel Chromium Fischer Carbenes
- Authors: Meyer, Annalene
- Date: 2012
- Subjects: Alkoxides , Organometallic compounds , Carbenes (Methylene compounds) , Chromium , Molybdenum , Tungsten , Organolithium compounds
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4371 , http://hdl.handle.net/10962/d1005036 , Alkoxides , Organometallic compounds , Carbenes (Methylene compounds) , Chromium , Molybdenum , Tungsten , Organolithium compounds
- Description: The present study involves the use of main group organometallics: organostannanes and organolithiums as precursors to chromium Fischer carbene complexes. Fischer carbenes are well stabilized by the π‐donor substituents such as alkoxy and amino groups and low oxidation state metals such as Group 6 (Chromium, Molybdenum or Tungsten). Carbenes are an important intermediate in the synthesis of a range of compounds through cyclopropanations, insertions, coupling and photochemical reactions. Synthesis and successful characterisation of three α‐alkoxystannanes was achieved via nucleophilic addition of tributylstannyllithium to the respective aldehydes, followed by an immediate MOM protection of the resulting alcohol. Six α‐aminostanannes were synthesised, consisting of N‐BOC, N‐acetyl and N‐ethyl derivatives of pyrrolidine and piperidine, via α‐lithiation and subsequent tinlithium transmetallation in the presence of TMEDA. The ¹³C NMR analysis highlighted an interesting phenomenon of tin‐carbon coupling that revealed unique structural information of both types of stannanes. DFT analysis was completed on the series of stannanes; a predicted frequency analysis was obtained which complemented the experimental Infra‐red data in elucidation of the compounds. The α‐alkoxy and α‐aminostannanes provided stable precursors to the organolithiums required for the synthesis of the novel Fischer chromium carbenes. The organolithiums were obtained via tinlithium exchange at low temperatures, followed by the addition of chromium hexacarbonyl to form the acylpentacarbonyl‐chromate salt. Alkylation of this intermediate using a Meerwein salt, Me₃OBF₄, gave rise to the novel Fischer chromium carbene complexes. Fischer chromium carbenes derived from the two isomeric butyl and isobutyl stannanes and the two N‐ethyl α‐aminostannanes were successfully synthesised. The difficulty encountered in the purification of the Fischer carbene complexes hindered the full characterisation, due to the presence of a by‐product, tetrabutyltin.
- Full Text:
- Date Issued: 2012
- Authors: Meyer, Annalene
- Date: 2012
- Subjects: Alkoxides , Organometallic compounds , Carbenes (Methylene compounds) , Chromium , Molybdenum , Tungsten , Organolithium compounds
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4371 , http://hdl.handle.net/10962/d1005036 , Alkoxides , Organometallic compounds , Carbenes (Methylene compounds) , Chromium , Molybdenum , Tungsten , Organolithium compounds
- Description: The present study involves the use of main group organometallics: organostannanes and organolithiums as precursors to chromium Fischer carbene complexes. Fischer carbenes are well stabilized by the π‐donor substituents such as alkoxy and amino groups and low oxidation state metals such as Group 6 (Chromium, Molybdenum or Tungsten). Carbenes are an important intermediate in the synthesis of a range of compounds through cyclopropanations, insertions, coupling and photochemical reactions. Synthesis and successful characterisation of three α‐alkoxystannanes was achieved via nucleophilic addition of tributylstannyllithium to the respective aldehydes, followed by an immediate MOM protection of the resulting alcohol. Six α‐aminostanannes were synthesised, consisting of N‐BOC, N‐acetyl and N‐ethyl derivatives of pyrrolidine and piperidine, via α‐lithiation and subsequent tinlithium transmetallation in the presence of TMEDA. The ¹³C NMR analysis highlighted an interesting phenomenon of tin‐carbon coupling that revealed unique structural information of both types of stannanes. DFT analysis was completed on the series of stannanes; a predicted frequency analysis was obtained which complemented the experimental Infra‐red data in elucidation of the compounds. The α‐alkoxy and α‐aminostannanes provided stable precursors to the organolithiums required for the synthesis of the novel Fischer chromium carbenes. The organolithiums were obtained via tinlithium exchange at low temperatures, followed by the addition of chromium hexacarbonyl to form the acylpentacarbonyl‐chromate salt. Alkylation of this intermediate using a Meerwein salt, Me₃OBF₄, gave rise to the novel Fischer chromium carbene complexes. Fischer chromium carbenes derived from the two isomeric butyl and isobutyl stannanes and the two N‐ethyl α‐aminostannanes were successfully synthesised. The difficulty encountered in the purification of the Fischer carbene complexes hindered the full characterisation, due to the presence of a by‐product, tetrabutyltin.
- Full Text:
- Date Issued: 2012
The treatment of brewery effluent using an integrated high rate algal ponding system
- Authors: Cilliers, Anneke
- Date: 2012
- Subjects: Water -- Purification -- South Africa , Algae -- Biotechnology , Algae culture , Algae -- Economic aspects
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5331 , http://hdl.handle.net/10962/d1005177 , Water -- Purification -- South Africa , Algae -- Biotechnology , Algae culture , Algae -- Economic aspects
- Description: The application of high rate algal ponds (HRAP) in the treatment of brewery effluent that met the South African Department of Water Affairs and Forestry's (DWAF) general limits for discharge into a natural water resource of 1998 were tested during a lO-month baseline phase, followed by an 11-month optimization phase. The objective of the baseline phase was to monitor the seasonal performance of HRAPs. The hydraulic retention time (HRT) fluctuated between 11.16 d and 12.00 d in HRAPs. The chemical oxygen demand (COD) increased from 130.12 ± 6.94 mg/L (post-AD), to 171.21 ± 7.99 mg/L (post-HRAP) . The presence of algal cells and evaporation contributed towards an increase in post-HRAP COD. The ammonia (NH₄-N) concentration decreased from 46.59 ± 2.47 mg/L (post-AD), to 1.08 ± 0.12 mg/L (post-HRAP). The nitrite (NO₂- N) concentration remained below 1.00 mg/L in post-pilot plant AD, post-PFP and post-HRAP effluent. The phosphate (PO₄-P) concentration decreased from 29.81 ± 1.39 mg/L (post-AD) to 17.30 ± 1.16 mg/L PO₄-P. The objective of the optimization phase was to manipulate the HRT to achieve the maximum treatment rate that met the DWAF general limits for discharge into a natural water resource of 1998. Nitrogen (as NH₄-N, NO₃-N, NO₂-N) removal efficiency was used as an indicator of nutrient removal success. HRT was influenced by season. The optimal HRT for autumn was 4.30 d at a temperature of 20.53ºC in HRAP A2 (heated) and 18.96ºC in HRAP B2 (ambient). The optimal HRT for summer was 2.74 d at 29.90ºC in HRAP A2 (heated) and 26.36ºC in HRAP B2 (ambient). The COD decreased from 152.33 ± 4.85 mg/L (post-AD) to 95 .00 ± 3.75 mg/L (post-HRAP A2), and to 100.82 ± 5.93 mg/L (post-HRAP B2). The incoming NH₄-N concentration decreased from 42.53 ± 1.38 mg/ L (post-AD), to 1.70 ± 0.81 mg/ L (post-HRAP) . The nitrate (NO₃-N) concentration post-HRAP was 12 - 14 mg/L. The main methods for NH₄-N removal were probably NH₄-N volatilization through algal uptake. HRAPs were able to lower nitrogen and phosphorous concentrations to within the DWAF limits under normal operating conditions. It is recommended that HRAP treated brewery wastewater be used for irrigation after salt removal, or alternatively, for groundwater recharge . Regulatory exemptions would be required for higher than permitted COD and EC concentrations to enable these actions.
- Full Text:
- Date Issued: 2012
- Authors: Cilliers, Anneke
- Date: 2012
- Subjects: Water -- Purification -- South Africa , Algae -- Biotechnology , Algae culture , Algae -- Economic aspects
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5331 , http://hdl.handle.net/10962/d1005177 , Water -- Purification -- South Africa , Algae -- Biotechnology , Algae culture , Algae -- Economic aspects
- Description: The application of high rate algal ponds (HRAP) in the treatment of brewery effluent that met the South African Department of Water Affairs and Forestry's (DWAF) general limits for discharge into a natural water resource of 1998 were tested during a lO-month baseline phase, followed by an 11-month optimization phase. The objective of the baseline phase was to monitor the seasonal performance of HRAPs. The hydraulic retention time (HRT) fluctuated between 11.16 d and 12.00 d in HRAPs. The chemical oxygen demand (COD) increased from 130.12 ± 6.94 mg/L (post-AD), to 171.21 ± 7.99 mg/L (post-HRAP) . The presence of algal cells and evaporation contributed towards an increase in post-HRAP COD. The ammonia (NH₄-N) concentration decreased from 46.59 ± 2.47 mg/L (post-AD), to 1.08 ± 0.12 mg/L (post-HRAP). The nitrite (NO₂- N) concentration remained below 1.00 mg/L in post-pilot plant AD, post-PFP and post-HRAP effluent. The phosphate (PO₄-P) concentration decreased from 29.81 ± 1.39 mg/L (post-AD) to 17.30 ± 1.16 mg/L PO₄-P. The objective of the optimization phase was to manipulate the HRT to achieve the maximum treatment rate that met the DWAF general limits for discharge into a natural water resource of 1998. Nitrogen (as NH₄-N, NO₃-N, NO₂-N) removal efficiency was used as an indicator of nutrient removal success. HRT was influenced by season. The optimal HRT for autumn was 4.30 d at a temperature of 20.53ºC in HRAP A2 (heated) and 18.96ºC in HRAP B2 (ambient). The optimal HRT for summer was 2.74 d at 29.90ºC in HRAP A2 (heated) and 26.36ºC in HRAP B2 (ambient). The COD decreased from 152.33 ± 4.85 mg/L (post-AD) to 95 .00 ± 3.75 mg/L (post-HRAP A2), and to 100.82 ± 5.93 mg/L (post-HRAP B2). The incoming NH₄-N concentration decreased from 42.53 ± 1.38 mg/ L (post-AD), to 1.70 ± 0.81 mg/ L (post-HRAP) . The nitrate (NO₃-N) concentration post-HRAP was 12 - 14 mg/L. The main methods for NH₄-N removal were probably NH₄-N volatilization through algal uptake. HRAPs were able to lower nitrogen and phosphorous concentrations to within the DWAF limits under normal operating conditions. It is recommended that HRAP treated brewery wastewater be used for irrigation after salt removal, or alternatively, for groundwater recharge . Regulatory exemptions would be required for higher than permitted COD and EC concentrations to enable these actions.
- Full Text:
- Date Issued: 2012
The trophic ecology of parrotfish of Zanzibar application of stable isotope analysis
- Plass-Johnson, Jeremiah Grahm
- Authors: Plass-Johnson, Jeremiah Grahm
- Date: 2012
- Subjects: Parrotfishes -- Tanzania -- Zanzibar , Parrotfishes , Parrotfishes -- Ecology -- Tanzania -- Zanzibar , Parrotfishes -- Behavior -- Tanzania -- Zanzibar , Parrotfishes -- Food -- Tanzania -- Zanzibar , Scleractinia -- Tanzania -- Zanzibar , Algae -- Tanzania -- Zanzibar , Coral reefs and islands -- Tanzania -- Zanzibar , Coral reef fishes -- Tanzania -- Zanzibar , Stable isotopes
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5789 , http://hdl.handle.net/10962/d1005477 , Parrotfishes -- Tanzania -- Zanzibar , Parrotfishes , Parrotfishes -- Ecology -- Tanzania -- Zanzibar , Parrotfishes -- Behavior -- Tanzania -- Zanzibar , Parrotfishes -- Food -- Tanzania -- Zanzibar , Scleractinia -- Tanzania -- Zanzibar , Algae -- Tanzania -- Zanzibar , Coral reefs and islands -- Tanzania -- Zanzibar , Coral reef fishes -- Tanzania -- Zanzibar , Stable isotopes
- Description: Parrotfish are a critical component of the herbivore functional group on tropical coral reefs around the world because they mediate competition that occurs between algae and scleractinian corals. Also, because of their feeding technique, which consists of rasping at the substratum with their beak-like teeth, they play an important role in carbonate turnover and the clearing of reef surface area for the settlement of new sessile organisms. Because of these roles, parrotfishes are an important structuring component of coral reef communities. However, individual species can play different roles depending on their physiology, behaviour and ecology. Despite the possible ecological differences that may exist amongst species, specific roles of the fishes remain unclear as the group is most often studied at higher community levels. This thesis applied stable isotope analysis to differing levels of organisation within a parrotfish community to help elucidate their trophic ecology on coral reefs in Zanzibar. Firstly, blood and muscle tissues were compared to identify differences in their isotope signatures. In other organisms, blood turns over faster than muscle tissue so that muscle tissue represents the diet as integrated over a longer period of time. In most species of parrotfish the blood and muscle δ¹³C signatures were not found to be significantly different, but the δ¹⁵N signatures were significantly different between tissues. This indicated that the δ¹³C signature of both tissues would reveal similar dietary information. Conversely, differences in the δ¹⁵N signature indicated that the nitrogen relationship between tissues was more complicated. Secondly, spatial variability in parrotfish, coral, detritus and macroalgae isotope signatures was assessed at different scales. In macroalgae and coral tissues (zooxanthellae and polyp treated separately), the δ¹³C signatures were shown to differ with depth, presumably because of changes in photosynthetic processes related to depth-associated changes in light. While δ¹⁵N signatures were not affected by depth, all organisms showed enrichment at the Nyange reef, the closest reef to the capital of Zanzibar, Stone Town, presumably reflecting the effects of sewage outfall. These results show that processes that impact the δ¹⁵N signatures of primary producers (macroalgae and zooxanthellae) can be traced to higher trophic levels (coral polyps and fish). Lastly, δ¹³C and δ¹⁵N signatures were used to identify ontogenetic dietary changes in multiple species of parrotfish. Four of the species showed stages that varied from the diets that are normally assumed on the basis of their dentition and feeding technique. This indicates that functional roles based on taxonomy or morphology may fail to include possible ontogenetic dietary changes, and may also fail to elucidate the full impact a species could have on coral reef communities. The conclusions from these studies indicate that the species-specific ecological role of parrotfish in coral reef communities can be complex within and between species, and may differ amongst reefs. In light of the natural and anthropogenic pressures that affect coral reef systems, management decisions based on a more complete understanding of the role of these fish in coral reef communities will help decisions that maintain resilience in these fragile systems.
- Full Text:
- Date Issued: 2012
- Authors: Plass-Johnson, Jeremiah Grahm
- Date: 2012
- Subjects: Parrotfishes -- Tanzania -- Zanzibar , Parrotfishes , Parrotfishes -- Ecology -- Tanzania -- Zanzibar , Parrotfishes -- Behavior -- Tanzania -- Zanzibar , Parrotfishes -- Food -- Tanzania -- Zanzibar , Scleractinia -- Tanzania -- Zanzibar , Algae -- Tanzania -- Zanzibar , Coral reefs and islands -- Tanzania -- Zanzibar , Coral reef fishes -- Tanzania -- Zanzibar , Stable isotopes
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5789 , http://hdl.handle.net/10962/d1005477 , Parrotfishes -- Tanzania -- Zanzibar , Parrotfishes , Parrotfishes -- Ecology -- Tanzania -- Zanzibar , Parrotfishes -- Behavior -- Tanzania -- Zanzibar , Parrotfishes -- Food -- Tanzania -- Zanzibar , Scleractinia -- Tanzania -- Zanzibar , Algae -- Tanzania -- Zanzibar , Coral reefs and islands -- Tanzania -- Zanzibar , Coral reef fishes -- Tanzania -- Zanzibar , Stable isotopes
- Description: Parrotfish are a critical component of the herbivore functional group on tropical coral reefs around the world because they mediate competition that occurs between algae and scleractinian corals. Also, because of their feeding technique, which consists of rasping at the substratum with their beak-like teeth, they play an important role in carbonate turnover and the clearing of reef surface area for the settlement of new sessile organisms. Because of these roles, parrotfishes are an important structuring component of coral reef communities. However, individual species can play different roles depending on their physiology, behaviour and ecology. Despite the possible ecological differences that may exist amongst species, specific roles of the fishes remain unclear as the group is most often studied at higher community levels. This thesis applied stable isotope analysis to differing levels of organisation within a parrotfish community to help elucidate their trophic ecology on coral reefs in Zanzibar. Firstly, blood and muscle tissues were compared to identify differences in their isotope signatures. In other organisms, blood turns over faster than muscle tissue so that muscle tissue represents the diet as integrated over a longer period of time. In most species of parrotfish the blood and muscle δ¹³C signatures were not found to be significantly different, but the δ¹⁵N signatures were significantly different between tissues. This indicated that the δ¹³C signature of both tissues would reveal similar dietary information. Conversely, differences in the δ¹⁵N signature indicated that the nitrogen relationship between tissues was more complicated. Secondly, spatial variability in parrotfish, coral, detritus and macroalgae isotope signatures was assessed at different scales. In macroalgae and coral tissues (zooxanthellae and polyp treated separately), the δ¹³C signatures were shown to differ with depth, presumably because of changes in photosynthetic processes related to depth-associated changes in light. While δ¹⁵N signatures were not affected by depth, all organisms showed enrichment at the Nyange reef, the closest reef to the capital of Zanzibar, Stone Town, presumably reflecting the effects of sewage outfall. These results show that processes that impact the δ¹⁵N signatures of primary producers (macroalgae and zooxanthellae) can be traced to higher trophic levels (coral polyps and fish). Lastly, δ¹³C and δ¹⁵N signatures were used to identify ontogenetic dietary changes in multiple species of parrotfish. Four of the species showed stages that varied from the diets that are normally assumed on the basis of their dentition and feeding technique. This indicates that functional roles based on taxonomy or morphology may fail to include possible ontogenetic dietary changes, and may also fail to elucidate the full impact a species could have on coral reef communities. The conclusions from these studies indicate that the species-specific ecological role of parrotfish in coral reef communities can be complex within and between species, and may differ amongst reefs. In light of the natural and anthropogenic pressures that affect coral reef systems, management decisions based on a more complete understanding of the role of these fish in coral reef communities will help decisions that maintain resilience in these fragile systems.
- Full Text:
- Date Issued: 2012
Towards a methodology for identifying potential sites for cemeteries
- Authors: Judge, Richard David Eadie
- Date: 2012
- Subjects: Cemeteries , Environmental impact analysis , Groundwater , Pollution
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10649 , http://hdl.handle.net/10948/d1008048 , Cemeteries , Environmental impact analysis , Groundwater , Pollution
- Description: Due to death being an extremely sensitive issue, the topic of cemeteries and associated environmental impacts is often left outside the mainstream environmental critique. However, this is a topic becoming more prevalent as the population numbers and the death rate increases, while the amount of available land decreases. Recent research has indicated that poorly sited cemeteries may pose a significant threat to groundwater resources with the consequential potential for severe health hazards. This has resulted in the need for a method of determining the acceptability of a given area for the establishment of a cemetery in a South African context. Cemeteries should be sited in such a way as to mitigate potential public health and safety concerns, minimise associated environmental impacts and provide a method of body disposal that is economically viable. This study therefore provides an integrated methodology to identify and assess a given area and rank a number of potential sites, ultimately determining a single cemetery site which proves to be acceptable for the establishment of a cemetery. Cemetery site selection should be based on the factors affecting the pollution potential of a proposed cemetery. These factors were identified and quantified based on research into the mechanisms of cemetery site pollution resulting in a number of fatal flaws and criteria deemed decisive when selecting a potential cemetery site. The assessment of a site with regards to these criteria and fatal flaws is undertaken through the use of GIS analysis software utilising data layers containing information on the site selection criteria, by investigating existing studies, literature or reports relating to the relevant area, or through field investigations. Although these criteria are vital when determining the specific characteristics of a site in terms of its pollution potential, a method of assessing a number of potential sites with regards to these criteria is vital. To this end, a multi-criteria ranking matrix has been developed, allowing for an objective method of assessing individual sites and thus indicating which sites are more suited for the establishment of a cemetery. The ranking matrix identifies a range of values for each criterion, therefore identifying a minimum and maximum allowable value. A site is then assessed with regards to these criteria in relation to the values identified in the ranking matrix. Each criteria is assigned a score according to the site conditions. Once the criteria for each site has been assessed and scored, the results can be tallied allowing the sites to be ranked according to which site proved to be the most acceptable for a cemetery based on the findings of the application of the site selection criteria. The methodology developed in this study is unique to previous studies in that it provides an integrated and staged approach to identifying, assessing and applying the criteria affecting the pollution potential of a cemetery. The methodology also provides a means of ranking a number of potential sites so to determine the most suitable. Furthermore, the criteria deemed as decisive in previous investigations were in most cases not quantified by the relevant authors, therefore leaving many of the criteria values up for interpretation. For this methodology to be affective, all criteria must be quantified therefore identifying maximum and minimum allowable limits for each. This study applies minimum and maximum allowable limits to these criteria, therefore aiding in the ranking process. The integrated methodology developed was then applied to a case study where by the effectiveness in identifying a number of potential cemetery sites could be tested. Subsequent to the application of this methodology to a case study, it was concluded that an additional two criteria, not identified in previous investigations, needed to be assessed to more adequately determine the suitability or otherwise of a site for a cemetery. Ultimately, twelve major criteria have been proposed for use as the basis of the methodology. The methodology and pertinent criteria proposed in this study should be compiled as a standard for planning authorities and consultants to use as a method of determining a number of potential environmentally sound cemetery sites.
- Full Text:
- Date Issued: 2012
- Authors: Judge, Richard David Eadie
- Date: 2012
- Subjects: Cemeteries , Environmental impact analysis , Groundwater , Pollution
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10649 , http://hdl.handle.net/10948/d1008048 , Cemeteries , Environmental impact analysis , Groundwater , Pollution
- Description: Due to death being an extremely sensitive issue, the topic of cemeteries and associated environmental impacts is often left outside the mainstream environmental critique. However, this is a topic becoming more prevalent as the population numbers and the death rate increases, while the amount of available land decreases. Recent research has indicated that poorly sited cemeteries may pose a significant threat to groundwater resources with the consequential potential for severe health hazards. This has resulted in the need for a method of determining the acceptability of a given area for the establishment of a cemetery in a South African context. Cemeteries should be sited in such a way as to mitigate potential public health and safety concerns, minimise associated environmental impacts and provide a method of body disposal that is economically viable. This study therefore provides an integrated methodology to identify and assess a given area and rank a number of potential sites, ultimately determining a single cemetery site which proves to be acceptable for the establishment of a cemetery. Cemetery site selection should be based on the factors affecting the pollution potential of a proposed cemetery. These factors were identified and quantified based on research into the mechanisms of cemetery site pollution resulting in a number of fatal flaws and criteria deemed decisive when selecting a potential cemetery site. The assessment of a site with regards to these criteria and fatal flaws is undertaken through the use of GIS analysis software utilising data layers containing information on the site selection criteria, by investigating existing studies, literature or reports relating to the relevant area, or through field investigations. Although these criteria are vital when determining the specific characteristics of a site in terms of its pollution potential, a method of assessing a number of potential sites with regards to these criteria is vital. To this end, a multi-criteria ranking matrix has been developed, allowing for an objective method of assessing individual sites and thus indicating which sites are more suited for the establishment of a cemetery. The ranking matrix identifies a range of values for each criterion, therefore identifying a minimum and maximum allowable value. A site is then assessed with regards to these criteria in relation to the values identified in the ranking matrix. Each criteria is assigned a score according to the site conditions. Once the criteria for each site has been assessed and scored, the results can be tallied allowing the sites to be ranked according to which site proved to be the most acceptable for a cemetery based on the findings of the application of the site selection criteria. The methodology developed in this study is unique to previous studies in that it provides an integrated and staged approach to identifying, assessing and applying the criteria affecting the pollution potential of a cemetery. The methodology also provides a means of ranking a number of potential sites so to determine the most suitable. Furthermore, the criteria deemed as decisive in previous investigations were in most cases not quantified by the relevant authors, therefore leaving many of the criteria values up for interpretation. For this methodology to be affective, all criteria must be quantified therefore identifying maximum and minimum allowable limits for each. This study applies minimum and maximum allowable limits to these criteria, therefore aiding in the ranking process. The integrated methodology developed was then applied to a case study where by the effectiveness in identifying a number of potential cemetery sites could be tested. Subsequent to the application of this methodology to a case study, it was concluded that an additional two criteria, not identified in previous investigations, needed to be assessed to more adequately determine the suitability or otherwise of a site for a cemetery. Ultimately, twelve major criteria have been proposed for use as the basis of the methodology. The methodology and pertinent criteria proposed in this study should be compiled as a standard for planning authorities and consultants to use as a method of determining a number of potential environmentally sound cemetery sites.
- Full Text:
- Date Issued: 2012
Towards integrated catchment management : challenges surrounding implementation in the Gamtoos River catchment
- Authors: Materechera, Fenji
- Date: 2012
- Subjects: Watershed management -- South Africa , Watershed management -- South Africa -- Citizen participation , Integrated water development -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10664 , http://hdl.handle.net/10948/d1018553
- Description: Water resource management has become a pertinent issue of global environmental concern in response to the conditions of a growing global population, increasing development and a limited freshwater supply. It is against the backdrop of such conditions that effective water resource management has gained popularity in seeking to ensure that the needs of the growing population will be met and secured for future generations. The notion of integrated water resource management (IWRM) is a perspective on water resource management that has evolved out of the global opinion that social and ecological systems are linked and therefore cannot be managed separately. The department of water affairs (DWA) in South Africa highlights the importance of approaching management of water resources from a catchment perspective which forms the basis for a particular integrated approach to management called integrated catchment management (ICM). ICM recognizes the catchment as the correct administrative unit for management. It integrates water resources and the land that forms the catchment area in planning and management. Researchers have described the implementation of ICM as being complicated and difficult. This is no exception to South Africa. Principles of ICM have received widespread prominence in South Africa as they have been incorporated into national water policy. Actual implementation however is still in its infancy. The study is therefore a case study of ICM with respect to factors influencing implementation amongst different stakeholders. The study aims to explore the theme of implementation of ICM within the context of the Gamtoos River Catchment with a view toward identifying and addressing challenges that may be more broadly applicable. The study adopts an inductive, exploratory approach to the connection between theory and practice. A systems-based framework characterized by sequential steps similar to that employed in a case study conducted by Bellamy et al. (2001) in Queensland Australia is used to facilitate the evaluation of ICM in the Gamtoos River Catchment. The evaluation is achieved through a three step process of exploration in the current study. Triangulation is applied to the choice of methods of analysis which involves the use of a global analysis method, the use of learning scenarios and a grounded theory method. Findings reveal seven core themes which help to provide a detailed, contextual understanding relating to the status quo for ICM in the catchment. Results from a grounded theory analysis summarized the main challenges to implementation into five broad categories. Based on this analysis method and the application of the three learning scenarios for the Gamtoos River Catchment, the extent to which these challenges exist was discovered. The state of ICM in the catchment was classified as falling within a condition of a level of success being achieved with room for improvement to a condition of optimal ICM. The study concludes that based on the context of ICM being an example of a Complex Adaptive Systems (CAS), this state of ICM in the Gamtoos River Catchment is subject to change. This therefore necessitates the consideration of approaches to implementation that are adaptive to change. Findings may serve to inform decision making on how ICM can be effectively implemented elsewhere in a South African context.
- Full Text:
- Date Issued: 2012
- Authors: Materechera, Fenji
- Date: 2012
- Subjects: Watershed management -- South Africa , Watershed management -- South Africa -- Citizen participation , Integrated water development -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10664 , http://hdl.handle.net/10948/d1018553
- Description: Water resource management has become a pertinent issue of global environmental concern in response to the conditions of a growing global population, increasing development and a limited freshwater supply. It is against the backdrop of such conditions that effective water resource management has gained popularity in seeking to ensure that the needs of the growing population will be met and secured for future generations. The notion of integrated water resource management (IWRM) is a perspective on water resource management that has evolved out of the global opinion that social and ecological systems are linked and therefore cannot be managed separately. The department of water affairs (DWA) in South Africa highlights the importance of approaching management of water resources from a catchment perspective which forms the basis for a particular integrated approach to management called integrated catchment management (ICM). ICM recognizes the catchment as the correct administrative unit for management. It integrates water resources and the land that forms the catchment area in planning and management. Researchers have described the implementation of ICM as being complicated and difficult. This is no exception to South Africa. Principles of ICM have received widespread prominence in South Africa as they have been incorporated into national water policy. Actual implementation however is still in its infancy. The study is therefore a case study of ICM with respect to factors influencing implementation amongst different stakeholders. The study aims to explore the theme of implementation of ICM within the context of the Gamtoos River Catchment with a view toward identifying and addressing challenges that may be more broadly applicable. The study adopts an inductive, exploratory approach to the connection between theory and practice. A systems-based framework characterized by sequential steps similar to that employed in a case study conducted by Bellamy et al. (2001) in Queensland Australia is used to facilitate the evaluation of ICM in the Gamtoos River Catchment. The evaluation is achieved through a three step process of exploration in the current study. Triangulation is applied to the choice of methods of analysis which involves the use of a global analysis method, the use of learning scenarios and a grounded theory method. Findings reveal seven core themes which help to provide a detailed, contextual understanding relating to the status quo for ICM in the catchment. Results from a grounded theory analysis summarized the main challenges to implementation into five broad categories. Based on this analysis method and the application of the three learning scenarios for the Gamtoos River Catchment, the extent to which these challenges exist was discovered. The state of ICM in the catchment was classified as falling within a condition of a level of success being achieved with room for improvement to a condition of optimal ICM. The study concludes that based on the context of ICM being an example of a Complex Adaptive Systems (CAS), this state of ICM in the Gamtoos River Catchment is subject to change. This therefore necessitates the consideration of approaches to implementation that are adaptive to change. Findings may serve to inform decision making on how ICM can be effectively implemented elsewhere in a South African context.
- Full Text:
- Date Issued: 2012