Managing an inclusive school: a case study of a pilot school in Swaziland
- Authors: Zimba, Zondani
- Date: 2012
- Subjects: Inclusive education -- Swaziland -- Mbabane Special education -- Swaziland -- Mbabane Learning disabled children -- Education -- Swaziland -- Mbabane Students with disabilities -- Swaziland -- Mbabane School management and organization -- Swaziland -- Mbabane
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1669 , http://hdl.handle.net/10962/d1003552
- Description: Inclusive Education (IE) reflects the values, ethos, and culture of an education system committed to excellence by promoting education opportunities for all learners. IE is about building a more just society and ensuring the right to education for all learners regardless of their individual characteristics or difficulties (UNESCO: 2007). The kingdom of Swaziland has committed itself to high quality basic education which provides equal opportunities for all children and youth. This is evident in the Swaziland National Constitution (2006). To promote Education for All (EFA) as stated in the constitution, an IE Policy has been developed and a draft policy is in place. The programme has been operational since 2006. There are nine pilot schools and four teachers from each school who have been trained on how to handle pupils with disabilities. The programme will be rolled out to 608 primary schools by 2015. In this research, the goal was to investigate how a School Management Board responds to the challenges of managing an Inclusive School. This research is a qualitative interpretive case study based in one of the pilot schools in Mbabane in Swaziland. The study used document analysis, semi‐structured interviews and focus groups to collect data. The study revealed that the school has done much in accommodating IE as there are changes in management structures and approaches, organizational culture and operating procedures. On the other hand, there are still significant challenges such as a lack of knowledge of inclusion and negativity on the part of learners and parents. Other challenges include inadequate training for educators and lack of suitable infrastructure. The study concludes by recommending improved staff development programmes, infrastructure upgrades, acquiring appropriate teaching and learning resources and employing multidisciplinary personnel.
- Full Text:
- Authors: Zimba, Zondani
- Date: 2012
- Subjects: Inclusive education -- Swaziland -- Mbabane Special education -- Swaziland -- Mbabane Learning disabled children -- Education -- Swaziland -- Mbabane Students with disabilities -- Swaziland -- Mbabane School management and organization -- Swaziland -- Mbabane
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1669 , http://hdl.handle.net/10962/d1003552
- Description: Inclusive Education (IE) reflects the values, ethos, and culture of an education system committed to excellence by promoting education opportunities for all learners. IE is about building a more just society and ensuring the right to education for all learners regardless of their individual characteristics or difficulties (UNESCO: 2007). The kingdom of Swaziland has committed itself to high quality basic education which provides equal opportunities for all children and youth. This is evident in the Swaziland National Constitution (2006). To promote Education for All (EFA) as stated in the constitution, an IE Policy has been developed and a draft policy is in place. The programme has been operational since 2006. There are nine pilot schools and four teachers from each school who have been trained on how to handle pupils with disabilities. The programme will be rolled out to 608 primary schools by 2015. In this research, the goal was to investigate how a School Management Board responds to the challenges of managing an Inclusive School. This research is a qualitative interpretive case study based in one of the pilot schools in Mbabane in Swaziland. The study used document analysis, semi‐structured interviews and focus groups to collect data. The study revealed that the school has done much in accommodating IE as there are changes in management structures and approaches, organizational culture and operating procedures. On the other hand, there are still significant challenges such as a lack of knowledge of inclusion and negativity on the part of learners and parents. Other challenges include inadequate training for educators and lack of suitable infrastructure. The study concludes by recommending improved staff development programmes, infrastructure upgrades, acquiring appropriate teaching and learning resources and employing multidisciplinary personnel.
- Full Text:
Mapping the most significant computer hacking events to a temporal computer attack model
- Van Heerden, Renier, Pieterse, Heloise, Irwin, Barry V W
- Authors: Van Heerden, Renier , Pieterse, Heloise , Irwin, Barry V W
- Date: 2012
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/429950 , vital:72654 , https://doi.org/10.1007/978-3-642-33332-3_21
- Description: This paper presents eight of the most significant computer hacking events (also known as computer attacks). These events were selected because of their unique impact, methodology, or other properties. A temporal computer attack model is presented that can be used to model computer based attacks. This model consists of the following stages: Target Identification, Reconnaissance, Attack, and Post-Attack Recon-naissance stages. The Attack stage is separated into: Ramp-up, Dam-age and Residue. This paper demonstrates how our eight significant hacking events are mapped to the temporal computer attack model. The temporal computer attack model becomes a valuable asset in the protection of critical infrastructure by being able to detect similar attacks earlier.
- Full Text:
- Authors: Van Heerden, Renier , Pieterse, Heloise , Irwin, Barry V W
- Date: 2012
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/429950 , vital:72654 , https://doi.org/10.1007/978-3-642-33332-3_21
- Description: This paper presents eight of the most significant computer hacking events (also known as computer attacks). These events were selected because of their unique impact, methodology, or other properties. A temporal computer attack model is presented that can be used to model computer based attacks. This model consists of the following stages: Target Identification, Reconnaissance, Attack, and Post-Attack Recon-naissance stages. The Attack stage is separated into: Ramp-up, Dam-age and Residue. This paper demonstrates how our eight significant hacking events are mapped to the temporal computer attack model. The temporal computer attack model becomes a valuable asset in the protection of critical infrastructure by being able to detect similar attacks earlier.
- Full Text:
Marx, Weber and NGOs:
- Authors: Helliker, Kirk D
- Date: 2012
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/144738 , vital:38375 , DOI: 10.1080/21528586.2007.10419171
- Description: This article offers a sociological understanding of intermediary NGOs in the modern world. In does so by drawing on certain epistemological insights of Marx and Weber, and this entails methodologies of both deconstruction and reconstruction. In arguing against a sociological behaviourism that pervades the NGO literature, the article conceptualises intermediary NGOs as a ‘social form’ embodying contradictory relations. For analytical purposes, the contradiction between ‘the global’ and ‘the local’ is brought to the fore.
- Full Text:
- Authors: Helliker, Kirk D
- Date: 2012
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/144738 , vital:38375 , DOI: 10.1080/21528586.2007.10419171
- Description: This article offers a sociological understanding of intermediary NGOs in the modern world. In does so by drawing on certain epistemological insights of Marx and Weber, and this entails methodologies of both deconstruction and reconstruction. In arguing against a sociological behaviourism that pervades the NGO literature, the article conceptualises intermediary NGOs as a ‘social form’ embodying contradictory relations. For analytical purposes, the contradiction between ‘the global’ and ‘the local’ is brought to the fore.
- Full Text:
Maternity Protection: discussion document 27-29 March 2012
- Congress of South African Trade Unions (COSATU)
- Authors: Congress of South African Trade Unions (COSATU)
- Date: 2012
- Subjects: Uncatalogued
- Language: English
- Type: book , text
- Identifier: http://hdl.handle.net/10962/68729 , vital:29309
- Description: Developed with the support of Solidarity Center (AFL-CIO) South Africa We would like to thank the COSATU Affiliates and Provinces for their contribution towards the development of this booklet. Special appreciation goes to the members of the COSATU National Gender Coordinating Committee (NGCC) and National Office Bearers designated to gender for their unwavering support towards the production of this booklet. Also our special gratitude goes to the following organisations who contributed towards compiling and development of the booklet:- • Commission for Gender Equality (CGE) • Gina Barbieri Attorneys • Legal Resource Centre(LRC) • African Diaspora Women Workers Network(ADWWN) • South African Self Employed Women’s Association(SASEWA) • International Labour Organisation (ILO).
- Full Text:
- Authors: Congress of South African Trade Unions (COSATU)
- Date: 2012
- Subjects: Uncatalogued
- Language: English
- Type: book , text
- Identifier: http://hdl.handle.net/10962/68729 , vital:29309
- Description: Developed with the support of Solidarity Center (AFL-CIO) South Africa We would like to thank the COSATU Affiliates and Provinces for their contribution towards the development of this booklet. Special appreciation goes to the members of the COSATU National Gender Coordinating Committee (NGCC) and National Office Bearers designated to gender for their unwavering support towards the production of this booklet. Also our special gratitude goes to the following organisations who contributed towards compiling and development of the booklet:- • Commission for Gender Equality (CGE) • Gina Barbieri Attorneys • Legal Resource Centre(LRC) • African Diaspora Women Workers Network(ADWWN) • South African Self Employed Women’s Association(SASEWA) • International Labour Organisation (ILO).
- Full Text:
Mechanistic studies of unusual Miruta-Baylis-Hillman reactions
- Authors: Nyoni, Dubekile
- Date: 2012
- Subjects: Chemical reactions Benzaldehyde Acrylonitrile Methyl acrylate Coumarins
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4400 , http://hdl.handle.net/10962/d1006692
- Description: This study has focussed on the application of synthetic, kinetic and exploratory theoretical methods in elucidating the reaction mechanisms of four Morita-Baylis-Hillman (MBH) type reactions, viz, i) the reactions of the disulfide 2,2'-dithiodibenzaldehyde with various activated alkenes in the presence of DBU and Ph₃P, ii) the reactions of chromone-3-carbaldehydes with MVK, iii) the reactions of chromone-2-carbaldehydes with acrylonitrile and iv) with methyl acrylate. Attention has also been given to the origin of the observed regioselectivity in Michaelis-Arbuzov reactions of 3-(halomethyl)coumarins. Cleavage of the sulfur-sulfur bond of aryl and heteroaryl disulfides by the nitrogen nucleophile DBU has been demonstrated, and a dramatic increase in the rate of tandem MBH and disulfide cleavage reactions of 2,2'-dithiodibenzaldehyde with the activated alkenes, MVK, EVK, acrylonitrile, methyl acrylate and tert-butyl acrylate has been achieved through the use of the dual organo-catalyst system, DBU-Ph₃P – an improvement accompanied by an increase in the yields of the isolated products. Detailed NMR-based kinetic studies have been conducted on the DBU-catalysed reactions of 2,2'-dithiodibenzaldehyde with MVK and methyl acrylate, and a theoretical kinetic model has been developed and complementary computational studies using Gaussian 03, at the DFT-B3LYP/6-31G(d) level of theory have provided valuable insights into the mechanism of these complex transformations. Reactions of chromone-3-carbaldehydes with MVK to afford chromone dimers and tricyclic products have been repeated, and a novel, intermediate MBH adduct has been isolated. The mechanisms of the competing pathways have been elucidated by DFT calculations and the development of a detailed theoretical kinetic model has ensued. Unusual transformations in MBH-type reactions of chromone-2-carbaldehydes with acrylonitrile and methyl acrylate have been explored and the structures of the unexpected products have been established using 1- and 2-D NMR, HRMS and X-ray crystallographic techniques. Attention has also been given to the synthesis of 3-(halomethyl)coumarins via the MBH reaction, and their subsequent Michaelis-Arbuzov reactions with triethyl phosphite. An exploratory study of the kinetics of the phosphonation reaction has been undertaken and the regio-selectivity of nucleophilic attack at the 4- and 1'-positions in the 3-chloro- and 3-(iodomethyl)coumarins has been investigated by calculating Mulliken charges, LUMO surfaces and Fukui condensed local softness functions.
- Full Text:
- Authors: Nyoni, Dubekile
- Date: 2012
- Subjects: Chemical reactions Benzaldehyde Acrylonitrile Methyl acrylate Coumarins
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4400 , http://hdl.handle.net/10962/d1006692
- Description: This study has focussed on the application of synthetic, kinetic and exploratory theoretical methods in elucidating the reaction mechanisms of four Morita-Baylis-Hillman (MBH) type reactions, viz, i) the reactions of the disulfide 2,2'-dithiodibenzaldehyde with various activated alkenes in the presence of DBU and Ph₃P, ii) the reactions of chromone-3-carbaldehydes with MVK, iii) the reactions of chromone-2-carbaldehydes with acrylonitrile and iv) with methyl acrylate. Attention has also been given to the origin of the observed regioselectivity in Michaelis-Arbuzov reactions of 3-(halomethyl)coumarins. Cleavage of the sulfur-sulfur bond of aryl and heteroaryl disulfides by the nitrogen nucleophile DBU has been demonstrated, and a dramatic increase in the rate of tandem MBH and disulfide cleavage reactions of 2,2'-dithiodibenzaldehyde with the activated alkenes, MVK, EVK, acrylonitrile, methyl acrylate and tert-butyl acrylate has been achieved through the use of the dual organo-catalyst system, DBU-Ph₃P – an improvement accompanied by an increase in the yields of the isolated products. Detailed NMR-based kinetic studies have been conducted on the DBU-catalysed reactions of 2,2'-dithiodibenzaldehyde with MVK and methyl acrylate, and a theoretical kinetic model has been developed and complementary computational studies using Gaussian 03, at the DFT-B3LYP/6-31G(d) level of theory have provided valuable insights into the mechanism of these complex transformations. Reactions of chromone-3-carbaldehydes with MVK to afford chromone dimers and tricyclic products have been repeated, and a novel, intermediate MBH adduct has been isolated. The mechanisms of the competing pathways have been elucidated by DFT calculations and the development of a detailed theoretical kinetic model has ensued. Unusual transformations in MBH-type reactions of chromone-2-carbaldehydes with acrylonitrile and methyl acrylate have been explored and the structures of the unexpected products have been established using 1- and 2-D NMR, HRMS and X-ray crystallographic techniques. Attention has also been given to the synthesis of 3-(halomethyl)coumarins via the MBH reaction, and their subsequent Michaelis-Arbuzov reactions with triethyl phosphite. An exploratory study of the kinetics of the phosphonation reaction has been undertaken and the regio-selectivity of nucleophilic attack at the 4- and 1'-positions in the 3-chloro- and 3-(iodomethyl)coumarins has been investigated by calculating Mulliken charges, LUMO surfaces and Fukui condensed local softness functions.
- Full Text:
Microalgae biomass as fermentation feedstock
- Authors: Tijjani-Oshungboye, Kubura
- Date: 2012
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5956 , http://hdl.handle.net/10962/d1006168
- Description: The search for alternative energy is as a result of pollution generated by the utilization of fossil fuel. Bearing in mind the increase in demand which exceeds supply, alternative energy must reduce the carbon foot print in order to relieve use of fossil fuels. Biogas generation from wastes is an old technology that has been in existence for decades. This same concept was behind the development of the integrated algae ponding system (IAPS), where the use of microalgae biomass is adopted for waste water treatment and, anaerobic digestion which is a component of the IAPS, simultaneously generates biogas. The biogas from the IAPS was quantified in order to evaluate efficiency of the system and the anaerobic fermentation pit was also simulated in the laboratory to optimize biogas production using microalgae as co-fermentation feedstock. Microalgae biomass was evaluated as potential feedstock for ethanol fermentation and the use of biogas was investigated as an alternative transportation fuel. In an IAPS substantial biomass is produced on an annual basis. For effective treatment of waste water and efficient nutrient removal continuous harvest of the biomass is required. In the present study, water treatment efficiency of the EBRU IAPS was determined by carrying out a series of tests to investigate the decline in nutrient content from port of influent entry to effluent discharge. There was more than a 60% reduction in nutrient content with a concomitant increase in biomass and growth rate of 0.25 g/L . Biogas generated from the IAPS was quantified using a flow meter and the composition determined by gas chromatography. Methane which is the principal constituent of biogas was 75% (±SD, n=IO) and 2.34 m³.d⁻¹ was measured as biogas yield from the EBRU IAPS. The study also investigated the use of the excess microalgae biomass as a fermentation feedstock for ethanol production and as a co-substrate in order to increase biogas yield from the system. Positive results were achieved for ethanol production from microalgae although yield was generally low. About 385 mg.⁻¹ of ethanol was recovered when glucose was used as substrate, where as only 115 mg.⁻¹ of ethanol was recovered with microalgae as substrate. Suitability of microalgae as feedstock for ethanol generation and biogas generation was determined by characterisation which involved estimation of the carbohydrate, protein and lipid content, and analysis of the C, H, 0, Nand S content. Laboratory fed batch reactors simulated the anaerobic digestion process in order to study the effect of microalgae biomass as co-substrate for biogas generation. The fermenters were inoculated with an active consortium obtained from the Makana municipal waste water works and microbial studies were carried to confirm the presence of the anaerobic consortium. Different pre-treatments (concentrated, rupturing and freeze-drying) were used to disrupt the microalgae prior to introduction into fermenters in a ratio of 3: I. COD, TC, TOC, SO₄⁻² and TN analyses were carried out to monitor nutrient depletion in the system, and biogas generated by the system was quantified by volumetric analysis and the gas composition determined. Statistical analysis (ANOVA) was used to test for significant difference pre and post addition of microalgae. In the most effective fermenter, biogas production was at an average of 394 ml.d·' and CH₄ ratio in the biogas increased by over a 100%. Theoretical methane potential of the IAPS and the Makana municipal waste water works treating 5 ML.d⁻¹ of domestic waste was determined using the empirical formula of waste water and shown to yield 1,037,342.40 m³/yr. The projected biogas yield from this system was used to evaluate its potential use as transportation fuel. In total, 198,673 .55 m³ of biogas was estimated to be required to fuel the Rhodes University's fleet of vehicles, with a residual biogas stream of 838,668.85 m³. It was also demonstrated in the present study that renewable energy sourced from biomass has the potential of supplanting the use of fossil fuel resulting in less pollution leading to a cleaner and healthier environment.
- Full Text:
- Authors: Tijjani-Oshungboye, Kubura
- Date: 2012
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5956 , http://hdl.handle.net/10962/d1006168
- Description: The search for alternative energy is as a result of pollution generated by the utilization of fossil fuel. Bearing in mind the increase in demand which exceeds supply, alternative energy must reduce the carbon foot print in order to relieve use of fossil fuels. Biogas generation from wastes is an old technology that has been in existence for decades. This same concept was behind the development of the integrated algae ponding system (IAPS), where the use of microalgae biomass is adopted for waste water treatment and, anaerobic digestion which is a component of the IAPS, simultaneously generates biogas. The biogas from the IAPS was quantified in order to evaluate efficiency of the system and the anaerobic fermentation pit was also simulated in the laboratory to optimize biogas production using microalgae as co-fermentation feedstock. Microalgae biomass was evaluated as potential feedstock for ethanol fermentation and the use of biogas was investigated as an alternative transportation fuel. In an IAPS substantial biomass is produced on an annual basis. For effective treatment of waste water and efficient nutrient removal continuous harvest of the biomass is required. In the present study, water treatment efficiency of the EBRU IAPS was determined by carrying out a series of tests to investigate the decline in nutrient content from port of influent entry to effluent discharge. There was more than a 60% reduction in nutrient content with a concomitant increase in biomass and growth rate of 0.25 g/L . Biogas generated from the IAPS was quantified using a flow meter and the composition determined by gas chromatography. Methane which is the principal constituent of biogas was 75% (±SD, n=IO) and 2.34 m³.d⁻¹ was measured as biogas yield from the EBRU IAPS. The study also investigated the use of the excess microalgae biomass as a fermentation feedstock for ethanol production and as a co-substrate in order to increase biogas yield from the system. Positive results were achieved for ethanol production from microalgae although yield was generally low. About 385 mg.⁻¹ of ethanol was recovered when glucose was used as substrate, where as only 115 mg.⁻¹ of ethanol was recovered with microalgae as substrate. Suitability of microalgae as feedstock for ethanol generation and biogas generation was determined by characterisation which involved estimation of the carbohydrate, protein and lipid content, and analysis of the C, H, 0, Nand S content. Laboratory fed batch reactors simulated the anaerobic digestion process in order to study the effect of microalgae biomass as co-substrate for biogas generation. The fermenters were inoculated with an active consortium obtained from the Makana municipal waste water works and microbial studies were carried to confirm the presence of the anaerobic consortium. Different pre-treatments (concentrated, rupturing and freeze-drying) were used to disrupt the microalgae prior to introduction into fermenters in a ratio of 3: I. COD, TC, TOC, SO₄⁻² and TN analyses were carried out to monitor nutrient depletion in the system, and biogas generated by the system was quantified by volumetric analysis and the gas composition determined. Statistical analysis (ANOVA) was used to test for significant difference pre and post addition of microalgae. In the most effective fermenter, biogas production was at an average of 394 ml.d·' and CH₄ ratio in the biogas increased by over a 100%. Theoretical methane potential of the IAPS and the Makana municipal waste water works treating 5 ML.d⁻¹ of domestic waste was determined using the empirical formula of waste water and shown to yield 1,037,342.40 m³/yr. The projected biogas yield from this system was used to evaluate its potential use as transportation fuel. In total, 198,673 .55 m³ of biogas was estimated to be required to fuel the Rhodes University's fleet of vehicles, with a residual biogas stream of 838,668.85 m³. It was also demonstrated in the present study that renewable energy sourced from biomass has the potential of supplanting the use of fossil fuel resulting in less pollution leading to a cleaner and healthier environment.
- Full Text:
Microbial monitoring of surface water in South Africa: an overview
- Luyt, Catherine D, Tandlich, Roman, Muller, Wilhelmine J, Wilhelmi, Brendan S
- Authors: Luyt, Catherine D , Tandlich, Roman , Muller, Wilhelmine J , Wilhelmi, Brendan S
- Date: 2012
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/71636 , vital:29927 , https://doi.org/10.3390/ijerph9082669
- Description: Infrastructural problems force South African households to supplement their drinking water consumption from water resources of inadequate microbial quality. Microbial water quality monitoring is currently based on the Colilert®18 system which leads to rapidly available results. Using Escherichia coli as the indicator microorganism limits the influence of environmental sources on the reported results. The current system allows for understanding of long-term trends of microbial surface water quality and the related public health risks. However, rates of false positive for the Colilert®18-derived concentrations have been reported to range from 7.4% to 36.4%. At the same time, rates of false negative results vary from 3.5% to 12.5%; and the Colilert medium has been reported to provide for cultivation of only 56.8% of relevant strains. Identification of unknown sources of faecal contamination is not currently feasible. Based on literature review, calibration of the antibiotic-resistance spectra of Escherichia coli or the bifidobacterial tracking ratio should be investigated locally for potential implementation into the existing monitoring system. The current system could be too costly to implement in certain areas of South Africa where the modified H2S strip test might be used as a surrogate for the Colilert®18.
- Full Text:
- Authors: Luyt, Catherine D , Tandlich, Roman , Muller, Wilhelmine J , Wilhelmi, Brendan S
- Date: 2012
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/71636 , vital:29927 , https://doi.org/10.3390/ijerph9082669
- Description: Infrastructural problems force South African households to supplement their drinking water consumption from water resources of inadequate microbial quality. Microbial water quality monitoring is currently based on the Colilert®18 system which leads to rapidly available results. Using Escherichia coli as the indicator microorganism limits the influence of environmental sources on the reported results. The current system allows for understanding of long-term trends of microbial surface water quality and the related public health risks. However, rates of false positive for the Colilert®18-derived concentrations have been reported to range from 7.4% to 36.4%. At the same time, rates of false negative results vary from 3.5% to 12.5%; and the Colilert medium has been reported to provide for cultivation of only 56.8% of relevant strains. Identification of unknown sources of faecal contamination is not currently feasible. Based on literature review, calibration of the antibiotic-resistance spectra of Escherichia coli or the bifidobacterial tracking ratio should be investigated locally for potential implementation into the existing monitoring system. The current system could be too costly to implement in certain areas of South Africa where the modified H2S strip test might be used as a surrogate for the Colilert®18.
- Full Text:
Mitochondrial DNA paradox: sex-specific genetic structure in a marine mussel despite maternal inheritance and passive dispersal
- Teske, Peter R, Papadopoulos, Isabelle, Barker, Nigel P, McQuaid, Christopher D
- Authors: Teske, Peter R , Papadopoulos, Isabelle , Barker, Nigel P , McQuaid, Christopher D
- Date: 2012
- Language: English
- Type: Article
- Identifier: vital:6836 , http://hdl.handle.net/10962/d1010959
- Description: Background: When genetic structure is identified using mitochondrial DNA (mtDNA), but no structure is identified using biparentally-inherited nuclear DNA, the discordance is often attributed to differences in dispersal potential between the sexes. Results: We sampled the intertidal rocky shore mussel Perna perna in a South African bay and along the nearby open coast, and sequenced maternally-inherited mtDNA (there is no evidence for paternally-inherited mtDNA in this species) and a biparentally-inherited marker. By treating males and females as different populations, we identified significant genetic structure on the basis of mtDNA data in the females only. Conclusions: This is the first study to report sex-specific differences in genetic structure based on matrilineally-inherited mtDNA in a passively dispersing species that lacks social structure or sexual dimorphism. The observed pattern most likely stems from females being more vulnerable to selection in habitats from which they did not originate, which also manifests itself in a male-biased sex ratio. Our results have three important implications for the interpretation of population genetic data. First, even when mtDNA is inherited exclusively in the female line, it also contains information about males. For that reason, using it to identify sex-specific differences in genetic structure by contrasting it with biparentally-inherited markers is problematic. Second, the fact that sex-specific differences were found in a passively dispersing species in which sex-biased dispersal is unlikely highlights the fact that significant genetic structure is not necessarily a function of low dispersal potential or physical barriers. Third, even though mtDNA is typically used to study historical demographic processes, it also contains information about contemporary processes. Higher survival rates of males in non-native habitats can erase the genetic structure present in their mothers within a single generation.
- Full Text:
- Authors: Teske, Peter R , Papadopoulos, Isabelle , Barker, Nigel P , McQuaid, Christopher D
- Date: 2012
- Language: English
- Type: Article
- Identifier: vital:6836 , http://hdl.handle.net/10962/d1010959
- Description: Background: When genetic structure is identified using mitochondrial DNA (mtDNA), but no structure is identified using biparentally-inherited nuclear DNA, the discordance is often attributed to differences in dispersal potential between the sexes. Results: We sampled the intertidal rocky shore mussel Perna perna in a South African bay and along the nearby open coast, and sequenced maternally-inherited mtDNA (there is no evidence for paternally-inherited mtDNA in this species) and a biparentally-inherited marker. By treating males and females as different populations, we identified significant genetic structure on the basis of mtDNA data in the females only. Conclusions: This is the first study to report sex-specific differences in genetic structure based on matrilineally-inherited mtDNA in a passively dispersing species that lacks social structure or sexual dimorphism. The observed pattern most likely stems from females being more vulnerable to selection in habitats from which they did not originate, which also manifests itself in a male-biased sex ratio. Our results have three important implications for the interpretation of population genetic data. First, even when mtDNA is inherited exclusively in the female line, it also contains information about males. For that reason, using it to identify sex-specific differences in genetic structure by contrasting it with biparentally-inherited markers is problematic. Second, the fact that sex-specific differences were found in a passively dispersing species in which sex-biased dispersal is unlikely highlights the fact that significant genetic structure is not necessarily a function of low dispersal potential or physical barriers. Third, even though mtDNA is typically used to study historical demographic processes, it also contains information about contemporary processes. Higher survival rates of males in non-native habitats can erase the genetic structure present in their mothers within a single generation.
- Full Text:
Mobile: the challenge of a unique new space for journalism: have you got your mojo?
- Authors: Malila, Vanessa
- Date: 2012
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/159517 , vital:40304 , https://hdl.handle.net/10520/EJC135832
- Description: The speed with which technology is improving in the mobile communications market is widening the scope for opportunity to engage with audiences, provide information and capture their attention for that little while longer. Current technology such as 3G wireless technologies, which allow for high-speed data transmission, and access to multimedia content, as well as smart phones and tablet computers have already illustrated the potential for users to access information that is tailored to mobile devices and available anywhere.
- Full Text:
- Authors: Malila, Vanessa
- Date: 2012
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/159517 , vital:40304 , https://hdl.handle.net/10520/EJC135832
- Description: The speed with which technology is improving in the mobile communications market is widening the scope for opportunity to engage with audiences, provide information and capture their attention for that little while longer. Current technology such as 3G wireless technologies, which allow for high-speed data transmission, and access to multimedia content, as well as smart phones and tablet computers have already illustrated the potential for users to access information that is tailored to mobile devices and available anywhere.
- Full Text:
Molecular catalysis of the oxygen reduction reaction by iron porphyrin catalysts tethered into Nafion layers
- He, Qinggang, Mugadza, Tawanda, Kang, Xiongwu, Zhu, Xiaobing, Chen, Shaowei, Kerr, John, Nyokong, Tebello
- Authors: He, Qinggang , Mugadza, Tawanda , Kang, Xiongwu , Zhu, Xiaobing , Chen, Shaowei , Kerr, John , Nyokong, Tebello
- Date: 2012
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/243168 , vital:51123 , xlink:href="https://doi.org/10.1016/j.jpowsour.2012.05.043"
- Description: This study was motivated by the need for improved understanding of the kinetics and transport phenomena in a homogeneous catalyst system for the oxygen reduction reaction (ORR). Direct interaction between the sulfonic groups of Nafion and an Fe(III) meso-tetra(N-methyl-4-pyridyl) porphine chloride (Fe(III)TMPyP) compound was observed using FTIR and in situ UV–Vis spectroelectrochemical characterizations. A positive shift of the half wave potential value (E1/2) for ORR on the iron porphyrin catalyst (Fe(III)TMPyP) was observed upon addition of a specific quantity of Nafion ionomer on a glassy carbon working electrode, indicating not only a faster charge transfer rate but also the role of protonation in the oxygen reduction reaction (ORR) process. A membrane electrode assembly (MEA) was made as a sandwich of a Pt-coated anode, a Nafion® 212 membrane, and a Fe(III)TMPyP + Nafion ionomer-coated cathode. This three-dimensional catalysis system has been demonstrated to be working in a H2/O2 proton exchange membrane (PEM) fuel cell test.
- Full Text:
- Authors: He, Qinggang , Mugadza, Tawanda , Kang, Xiongwu , Zhu, Xiaobing , Chen, Shaowei , Kerr, John , Nyokong, Tebello
- Date: 2012
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/243168 , vital:51123 , xlink:href="https://doi.org/10.1016/j.jpowsour.2012.05.043"
- Description: This study was motivated by the need for improved understanding of the kinetics and transport phenomena in a homogeneous catalyst system for the oxygen reduction reaction (ORR). Direct interaction between the sulfonic groups of Nafion and an Fe(III) meso-tetra(N-methyl-4-pyridyl) porphine chloride (Fe(III)TMPyP) compound was observed using FTIR and in situ UV–Vis spectroelectrochemical characterizations. A positive shift of the half wave potential value (E1/2) for ORR on the iron porphyrin catalyst (Fe(III)TMPyP) was observed upon addition of a specific quantity of Nafion ionomer on a glassy carbon working electrode, indicating not only a faster charge transfer rate but also the role of protonation in the oxygen reduction reaction (ORR) process. A membrane electrode assembly (MEA) was made as a sandwich of a Pt-coated anode, a Nafion® 212 membrane, and a Fe(III)TMPyP + Nafion ionomer-coated cathode. This three-dimensional catalysis system has been demonstrated to be working in a H2/O2 proton exchange membrane (PEM) fuel cell test.
- Full Text:
Molecular chaperone expression and function in breast cancer and breast cancer stem cells
- Authors: Sterrenberg, Jason Neville
- Date: 2012
- Subjects: Breast -- Cancer , Stem cells , Cancer cells
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4141 , http://hdl.handle.net/10962/d1016238
- Description: The Cancer Stem Cell (CSC) theory suggests that cancers arise from and are maintained by a subpopulation of cancer cells with stem cell properties. Molecular chaperones are key components of cellular regulation. The overexpression of chaperones has become synonymous with cancer cells with chaperones being recognized as bona fide anti-cancer drug targets. Although chaperone activity has been characterized in cancer cells, very little is known about the cellular functions of chaperones in cancer stem cells. We set out to compare the expression of selected molecular chaperones in non-stem cancer cell and cancer stem cell enriched populations isolated from breast cancer lines, in order to identify chaperones differentially expressed between the two populations for further biological characterization. In order to isolate breast cancer stem cells from the MCF-7 and MDA-MB-231 breast cancer cell lines, three cancer stem cell isolation and identification techniques were utilized based on (1) cell surface marker expression (CD44+/CD24- and CD44+/CD24-/EpCAM+ phenotypes), (2) aldehyde dehydrogenase enzyme activity (ALDHHi) and (3) ability to grow in anchorage-independent conditions. The MDA-MB-231 and MCF-7 breast cancer cell lines displayed CD44+/CD24- cell populations with the MCF-7 cell line additionally displaying a large CD44+/CD24-/EpCAM+ population. Although both cell lines showed similar ALDHHi populations, they differed substantially with respect to anchorage-independent growth. MCF-7 cells were able to form anchorage-independent colonies while the MDA-MB-231 cell line was not. Anchorage-independent MCF-7 cells showed enrichment in CD44+/CD24- and CD44+/CD24-/EpCAM+ cells compared to adherent MCF-7 cells, and were selected for gene expression studies. Gene expression studies identified 22 genes as being down-regulated at the mRNA level in the anchorage-independent MCF-7 cells, while only 2 genes (BAG1 and DNAJC12) were up-regulated. The down-regulation of selected chaperones in anchorage independent MCF-7 cells was confirmed at the protein level for selected chaperones, including DNAJB6, a type II DNAJ protein shown to be involved in the regulation of Wnt signaling. In order to characterize the effect of DNAJB6 expression on BCSCs we developed a pCMV mammalian expression plasmid for both DNAJB6 isoforms (DNAJB6L and DNAJB6S). We successfully constructed mutants of the conserved histidine-proline-aspartic acid (HPD) motif of the J domain of DNAJB6S and DNAJB6L. These constructs will allow the analysis of the role of DNAJB6 in cancer stem cell function. To the best of our knowledge, this is the first report to focus on the comparative expression of molecular chaperones in normal and cancer stem cell enriched breast cancer populations.
- Full Text:
- Authors: Sterrenberg, Jason Neville
- Date: 2012
- Subjects: Breast -- Cancer , Stem cells , Cancer cells
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4141 , http://hdl.handle.net/10962/d1016238
- Description: The Cancer Stem Cell (CSC) theory suggests that cancers arise from and are maintained by a subpopulation of cancer cells with stem cell properties. Molecular chaperones are key components of cellular regulation. The overexpression of chaperones has become synonymous with cancer cells with chaperones being recognized as bona fide anti-cancer drug targets. Although chaperone activity has been characterized in cancer cells, very little is known about the cellular functions of chaperones in cancer stem cells. We set out to compare the expression of selected molecular chaperones in non-stem cancer cell and cancer stem cell enriched populations isolated from breast cancer lines, in order to identify chaperones differentially expressed between the two populations for further biological characterization. In order to isolate breast cancer stem cells from the MCF-7 and MDA-MB-231 breast cancer cell lines, three cancer stem cell isolation and identification techniques were utilized based on (1) cell surface marker expression (CD44+/CD24- and CD44+/CD24-/EpCAM+ phenotypes), (2) aldehyde dehydrogenase enzyme activity (ALDHHi) and (3) ability to grow in anchorage-independent conditions. The MDA-MB-231 and MCF-7 breast cancer cell lines displayed CD44+/CD24- cell populations with the MCF-7 cell line additionally displaying a large CD44+/CD24-/EpCAM+ population. Although both cell lines showed similar ALDHHi populations, they differed substantially with respect to anchorage-independent growth. MCF-7 cells were able to form anchorage-independent colonies while the MDA-MB-231 cell line was not. Anchorage-independent MCF-7 cells showed enrichment in CD44+/CD24- and CD44+/CD24-/EpCAM+ cells compared to adherent MCF-7 cells, and were selected for gene expression studies. Gene expression studies identified 22 genes as being down-regulated at the mRNA level in the anchorage-independent MCF-7 cells, while only 2 genes (BAG1 and DNAJC12) were up-regulated. The down-regulation of selected chaperones in anchorage independent MCF-7 cells was confirmed at the protein level for selected chaperones, including DNAJB6, a type II DNAJ protein shown to be involved in the regulation of Wnt signaling. In order to characterize the effect of DNAJB6 expression on BCSCs we developed a pCMV mammalian expression plasmid for both DNAJB6 isoforms (DNAJB6L and DNAJB6S). We successfully constructed mutants of the conserved histidine-proline-aspartic acid (HPD) motif of the J domain of DNAJB6S and DNAJB6L. These constructs will allow the analysis of the role of DNAJB6 in cancer stem cell function. To the best of our knowledge, this is the first report to focus on the comparative expression of molecular chaperones in normal and cancer stem cell enriched breast cancer populations.
- Full Text:
Molecular phylogenetics of Oestroidea (Diptera: Calyptratae) with emphasis on Calliphoridae: insights into the inter-familial relationships and additional evidence for paraphyly among blowflies
- Marinho, M A T, Junqueira, A C M, Paulo, D F, Esposito, M C T, Villet, Martin H, Azeredo-Espin, A D
- Authors: Marinho, M A T , Junqueira, A C M , Paulo, D F , Esposito, M C T , Villet, Martin H , Azeredo-Espin, A D
- Date: 2012
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/442691 , vital:74023 , https://doi.org/10.1016/j.ympev.2012.08.007
- Description: The superfamily Oestroidea, comprising ∼15,000 species, is a large and ecologically diverse clade within the order Diptera. Among its six commonly recognized families, Calliphoridae seems to be crucial for understanding evolutionary relationships in the group, as it is recognized as a controversial paraphyletic grouping. To further investigate this matter, the ITS2, 28S, COI and 16S regions were used to infer phylogenetic relationships in Oestroidea with maximum-parsimony (MP), maximum-likelihood (ML) and Bayesian inference (BI) methods. For the BI analyses, a deep evaluation of different data partitioning strategies was conducted, including consideration of structural conformation (ITS2 and 16S) and codon position (COI) information.
- Full Text:
- Authors: Marinho, M A T , Junqueira, A C M , Paulo, D F , Esposito, M C T , Villet, Martin H , Azeredo-Espin, A D
- Date: 2012
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/442691 , vital:74023 , https://doi.org/10.1016/j.ympev.2012.08.007
- Description: The superfamily Oestroidea, comprising ∼15,000 species, is a large and ecologically diverse clade within the order Diptera. Among its six commonly recognized families, Calliphoridae seems to be crucial for understanding evolutionary relationships in the group, as it is recognized as a controversial paraphyletic grouping. To further investigate this matter, the ITS2, 28S, COI and 16S regions were used to infer phylogenetic relationships in Oestroidea with maximum-parsimony (MP), maximum-likelihood (ML) and Bayesian inference (BI) methods. For the BI analyses, a deep evaluation of different data partitioning strategies was conducted, including consideration of structural conformation (ITS2 and 16S) and codon position (COI) information.
- Full Text:
Mortgage bonds and the right of access to adequate housing in South Africa: Gundwana v Stoke Development and Others 2011 (3) SA 608 (CC)
- Authors: Juma, Laurence
- Date: 2012
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/127479 , vital:36015 , https://hdl.handle.net/10520/EJC129329
- Description: This article offers a critique of Gundwana v Stoke Development and Others 2011 (3) SA 608 (CC), a case in which the Constitutional Court of South Africa found it to be unconstitutional for the registrar of a high court to declare immovable properties specially executable when ordering a default judgement, to the extent that such an order "permitted the sale and execution of a home of a person". The Court interpreted the property clause in section 25, access to right to housing in section 26 of the Constitution, as mandating "further judicial oversight" in all cases where execution is levied against residential property. The article raises some of the shortcomings of this interpretive scheme and suggests that constitutional values, when used to curtail or enlarge obligations of parties to a mortgage bond, must take into account the general rights and duties which the parties assumed at the signing of the agreement; the circumstances of each of the parties at the time of execution and ascertained through a careful evaluation based on a clearly articulated set of principles, and the nature of constitutional rights themselves. The article argues that, whereas there may be circumstances in which a debtor may need protection, rather than impose a blanket abrogation of procedures allowing for expedient disposal of uncontested claims, the court should instead have considered the establishment of further procedural safeguards.
- Full Text: false
- Authors: Juma, Laurence
- Date: 2012
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/127479 , vital:36015 , https://hdl.handle.net/10520/EJC129329
- Description: This article offers a critique of Gundwana v Stoke Development and Others 2011 (3) SA 608 (CC), a case in which the Constitutional Court of South Africa found it to be unconstitutional for the registrar of a high court to declare immovable properties specially executable when ordering a default judgement, to the extent that such an order "permitted the sale and execution of a home of a person". The Court interpreted the property clause in section 25, access to right to housing in section 26 of the Constitution, as mandating "further judicial oversight" in all cases where execution is levied against residential property. The article raises some of the shortcomings of this interpretive scheme and suggests that constitutional values, when used to curtail or enlarge obligations of parties to a mortgage bond, must take into account the general rights and duties which the parties assumed at the signing of the agreement; the circumstances of each of the parties at the time of execution and ascertained through a careful evaluation based on a clearly articulated set of principles, and the nature of constitutional rights themselves. The article argues that, whereas there may be circumstances in which a debtor may need protection, rather than impose a blanket abrogation of procedures allowing for expedient disposal of uncontested claims, the court should instead have considered the establishment of further procedural safeguards.
- Full Text: false
Movement patterns, stock delineation and conservation of an overexploited fishery species, Lithognathus Lithognathus (Pisces: Sparidae)
- Authors: Bennett, Rhett Hamilton
- Date: 2012
- Subjects: Reef fishes -- Behavior , Endangered species -- South Africa , Fish stock assessment -- South Africa , Fishery management -- South Africa , Fish communities -- South Africa , Sparidae , Lithognathus , Lithognathus -- Growth
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5374 , http://hdl.handle.net/10962/d1015709
- Description: White steenbras Lithognathus lithognathus (Pisces: Sparidae) has been a major target species of numerous fisheries in South Africa, since the late 19th century. Historically, it contributed substantially to annual catches in commercial net fisheries, and became dominant in recreational shore catches in the latter half of the 20th century. However, overexploitation in both sectors resulted in severe declines in abundance. The ultimate collapse of the stock by the end of the last century, and the failure of traditional management measures to protect the species indicate that a new management approach for this species is necessary. The species was identified as a priority for research, management and conservation in a National Linefish Status Report. Despite knowledge on aspects of its biology and life history, little is known about juvenile habitat use patterns, home range dynamics and movement behaviour in estuaries. Similarly, the movement and migration of larger juveniles and adults in the marine environment are poorly understood. Furthermore, there is a complete lack of information on its genetic stock structure. Such information is essential for effective management of a fishery species. This thesis aimed to address the gaps in the understanding of white steenbras movement patterns and genetic stock structure, and provide an assessment of its current conservation status. The study adopted a multidisciplinary approach, incorporating a range of methods and drawing on available information, including published literature, unpublished reports and data from long-term monitoring programmes. Acoustic telemetry, conducted in a range of estuaries, showed high site fidelity, restricted area use, small home ranges relative to the size of the estuary, and a high level of residency within estuaries at the early juvenile life stage. Behaviour within estuaries was dominated by station-keeping, superimposed by a strong diel behaviour, presumably based on feeding and/or predator avoidance, with individuals entering the shallow littoral zone at night to feed, and seeking refuge in the deeper channel areas during the daytime. Conventional dart tagging and recapture data from four ongoing, long-term coastal fish tagging projects, spread throughout the distribution of this species, indicated high levels of residency in the surf zone at the late juvenile and sub-adult life stages. Consequently, juvenile and sub-adult white steenbras are vulnerable to localised depletion, although they can be effectively protected by suitably positioned estuarine protected areas (EPAs) and marine protected areas (MPAs), respectively. It has been hypothesized that adult white steenbras undertake large-scale coastal migrations between summer aggregation areas and winter spawning grounds. The scale of observed coastal movements was correlated with fish size (and age), with larger fish undertaking considerably longer-distance coastal movements than smaller individuals, supporting this hypothesis. Given the migratory behaviour of adults, and indications that limited spawning habitat exists, MPAs designed to protect white steenbras during the adult life stage should encompass all known spawning aggregation sites. The fishery is plagued by problems such as low compliance and low enforcement capacity, and alternative management measures, such as seasonal closure, need to be evaluated. Despite considerable conventional dart tagging effort around the coastline (5 782 fish tagged) with 292 recaptures there remains a lack of empirical evidence of fish migrating long distances (> 600 km) between aggregation and spawning areas. This uncertainty in the level of connectivity among coastal regions was addressed using mitochondrial DNA sequencing and genotyping of microsatellite repeat loci in the nuclear genome, which showed no evidence of major geographic barriers to gene flow in this species. Samples collected throughout the white steenbras core distribution showed high genetic diversity, low genetic differentiation and no evidence of isolation by distance or localised spawning. Although historically dominant in several fisheries, analysis of long-term commercial and recreational catch data for white steenbras indicated considerable declines and ultimately stock collapse. Improved catch-per-unit-effort in two large MPAs subsequent to closure confirmed that MPAs can be effective for the protection of white steenbras. However, the current MPA network encompasses a low proportion of sandy shoreline, for which white steenbras exhibits an affinity. Many MPAs do not prohibit recreational shore angling, which currently accounts for the greatest proportion of the total annual catch. Furthermore, EPAs within the juvenile distribution protect a negligible proportion of the total available surface area of estuaries – habitat on which white steenbras is wholly dependent. Despite some evidence of recent increases in abundance in estuaries and the surf zone in certain areas, white steenbras meets the criteria for “Endangered” on the IUCN Red List of Threatened Species, and for “Protected species” status on the National Environmental Management: Biodiversity Act of South Africa. The species requires improved management, with consideration for its life-history style, estuarine dependency, surf zone residency, predictable spawning migrations and its poor conservation status. The multidisciplinary approach provides valuable information towards an improved scientific basis for the management of white steenbras and a framework for research that can be adopted for other overexploited, estuarine-associated coastal fishery species.
- Full Text:
- Authors: Bennett, Rhett Hamilton
- Date: 2012
- Subjects: Reef fishes -- Behavior , Endangered species -- South Africa , Fish stock assessment -- South Africa , Fishery management -- South Africa , Fish communities -- South Africa , Sparidae , Lithognathus , Lithognathus -- Growth
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5374 , http://hdl.handle.net/10962/d1015709
- Description: White steenbras Lithognathus lithognathus (Pisces: Sparidae) has been a major target species of numerous fisheries in South Africa, since the late 19th century. Historically, it contributed substantially to annual catches in commercial net fisheries, and became dominant in recreational shore catches in the latter half of the 20th century. However, overexploitation in both sectors resulted in severe declines in abundance. The ultimate collapse of the stock by the end of the last century, and the failure of traditional management measures to protect the species indicate that a new management approach for this species is necessary. The species was identified as a priority for research, management and conservation in a National Linefish Status Report. Despite knowledge on aspects of its biology and life history, little is known about juvenile habitat use patterns, home range dynamics and movement behaviour in estuaries. Similarly, the movement and migration of larger juveniles and adults in the marine environment are poorly understood. Furthermore, there is a complete lack of information on its genetic stock structure. Such information is essential for effective management of a fishery species. This thesis aimed to address the gaps in the understanding of white steenbras movement patterns and genetic stock structure, and provide an assessment of its current conservation status. The study adopted a multidisciplinary approach, incorporating a range of methods and drawing on available information, including published literature, unpublished reports and data from long-term monitoring programmes. Acoustic telemetry, conducted in a range of estuaries, showed high site fidelity, restricted area use, small home ranges relative to the size of the estuary, and a high level of residency within estuaries at the early juvenile life stage. Behaviour within estuaries was dominated by station-keeping, superimposed by a strong diel behaviour, presumably based on feeding and/or predator avoidance, with individuals entering the shallow littoral zone at night to feed, and seeking refuge in the deeper channel areas during the daytime. Conventional dart tagging and recapture data from four ongoing, long-term coastal fish tagging projects, spread throughout the distribution of this species, indicated high levels of residency in the surf zone at the late juvenile and sub-adult life stages. Consequently, juvenile and sub-adult white steenbras are vulnerable to localised depletion, although they can be effectively protected by suitably positioned estuarine protected areas (EPAs) and marine protected areas (MPAs), respectively. It has been hypothesized that adult white steenbras undertake large-scale coastal migrations between summer aggregation areas and winter spawning grounds. The scale of observed coastal movements was correlated with fish size (and age), with larger fish undertaking considerably longer-distance coastal movements than smaller individuals, supporting this hypothesis. Given the migratory behaviour of adults, and indications that limited spawning habitat exists, MPAs designed to protect white steenbras during the adult life stage should encompass all known spawning aggregation sites. The fishery is plagued by problems such as low compliance and low enforcement capacity, and alternative management measures, such as seasonal closure, need to be evaluated. Despite considerable conventional dart tagging effort around the coastline (5 782 fish tagged) with 292 recaptures there remains a lack of empirical evidence of fish migrating long distances (> 600 km) between aggregation and spawning areas. This uncertainty in the level of connectivity among coastal regions was addressed using mitochondrial DNA sequencing and genotyping of microsatellite repeat loci in the nuclear genome, which showed no evidence of major geographic barriers to gene flow in this species. Samples collected throughout the white steenbras core distribution showed high genetic diversity, low genetic differentiation and no evidence of isolation by distance or localised spawning. Although historically dominant in several fisheries, analysis of long-term commercial and recreational catch data for white steenbras indicated considerable declines and ultimately stock collapse. Improved catch-per-unit-effort in two large MPAs subsequent to closure confirmed that MPAs can be effective for the protection of white steenbras. However, the current MPA network encompasses a low proportion of sandy shoreline, for which white steenbras exhibits an affinity. Many MPAs do not prohibit recreational shore angling, which currently accounts for the greatest proportion of the total annual catch. Furthermore, EPAs within the juvenile distribution protect a negligible proportion of the total available surface area of estuaries – habitat on which white steenbras is wholly dependent. Despite some evidence of recent increases in abundance in estuaries and the surf zone in certain areas, white steenbras meets the criteria for “Endangered” on the IUCN Red List of Threatened Species, and for “Protected species” status on the National Environmental Management: Biodiversity Act of South Africa. The species requires improved management, with consideration for its life-history style, estuarine dependency, surf zone residency, predictable spawning migrations and its poor conservation status. The multidisciplinary approach provides valuable information towards an improved scientific basis for the management of white steenbras and a framework for research that can be adopted for other overexploited, estuarine-associated coastal fishery species.
- Full Text:
Movements of sub-adult sicklefin lemon sharks Negaprion acutidens in a remote Indian Ocean atoll
- Authors: Filmalter, John David
- Date: 2012
- Subjects: Silky shark , Silky shark -- Habitat , Sharks -- Amirante Islands (Seychelles)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5383 , http://hdl.handle.net/10962/d1018229
- Description: The sicklefin lemon shark Negaprion acutidens is a large-bodied (> 3m) coastal shark species, widely distributed in tropical and sub-tropical Indo-Pacific waters. Despite its large size and wide distribution, very little is known about its movement behaviour throughout ontogeny. The primary aim of this thesis was to gain an understanding of the movements of sub-adult N. acutidens, in and around the St. Joseph Atoll, Republic of Seychelles, to facilitate the effective conservation of this vulnerable species. A total of 22 sharks (139 – 202 cm TL) were equipped with coded acoustic transmitters and passively monitored using moored acoustic receivers for a period of 20 month from March 2009 to October 2010. The use of passive acoustic telemetry arrays to study behavioural ecology has increased in popularity in recent years and been successfully applied to study a host of teleost and elasmobranch species in a diversity of habitats, ranging from sheltered estuaries to the offshore pelagic environment. However, the practicalities of designing an effective network of receivers to optimally monitor animal movements can be challenging. In this study the receiver array was optimised through a series of incremental steps to achieve an efficient design that allowed for the specific objectives to be addressed. The specific objectives of this study were to i) gain an understanding of the long term space use patterns and site fidelity of sub-adult N. acutidens within degree of use of the St. Joseph Atoll lagoon and surrounding habitats, and ii) investigate their short term behavioural patterns in response to natural rhythmic cycles. The important role the atoll plays in the sub-adult stage of this species was demonstrated by the very low numbers of detections outside the atoll compared to the very large numbers of detections inside the atoll. Over the course of a year the tagged sharks showed a high degree of site fidelity to the atoll, with 79 – 100% of the individuals detected during each month and 50% being detected on a near daily basis. This result provides testimony of the nursery role of the atoll and importance of these habitats in the early life history for this species. Individual area use was generally found to be restricted to the eastern part of the atoll incorporating a portion of the central deep lagoon and a nearby adjacent area of the sand flats. The movements of sub-adult N. acutidens on and off of the shallow flats surrounding the central lagoon (likely for foraging) were found to be strongly influenced by the tidal height and the diel cycle. Tagged sharks were found to utilise the flats more regularly when the tidal height was greater than 90 cm and particularly more so during the night and early morning than during daylight times. The combined results of this study expose the vulnerability of this species to rapid local depletion. Similarly, the effectiveness of spatial management measures, such as no-take marine protected areas is highlighted as an efficient tool for the future conservation of this species. In the St. Joseph Atoll in particular, the use of a multi-levelled protection approach, where the eastern portion on the atoll is completely restricted while the remaining area is utilized for non-consumptive tourism activities, could be highly effective. Future research should investigate changes in area use throughout the ontogeny of this species as well improve the understanding of the breeding site fidelity and population size of reproductive females using the St. Joseph Atoll.
- Full Text:
- Authors: Filmalter, John David
- Date: 2012
- Subjects: Silky shark , Silky shark -- Habitat , Sharks -- Amirante Islands (Seychelles)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5383 , http://hdl.handle.net/10962/d1018229
- Description: The sicklefin lemon shark Negaprion acutidens is a large-bodied (> 3m) coastal shark species, widely distributed in tropical and sub-tropical Indo-Pacific waters. Despite its large size and wide distribution, very little is known about its movement behaviour throughout ontogeny. The primary aim of this thesis was to gain an understanding of the movements of sub-adult N. acutidens, in and around the St. Joseph Atoll, Republic of Seychelles, to facilitate the effective conservation of this vulnerable species. A total of 22 sharks (139 – 202 cm TL) were equipped with coded acoustic transmitters and passively monitored using moored acoustic receivers for a period of 20 month from March 2009 to October 2010. The use of passive acoustic telemetry arrays to study behavioural ecology has increased in popularity in recent years and been successfully applied to study a host of teleost and elasmobranch species in a diversity of habitats, ranging from sheltered estuaries to the offshore pelagic environment. However, the practicalities of designing an effective network of receivers to optimally monitor animal movements can be challenging. In this study the receiver array was optimised through a series of incremental steps to achieve an efficient design that allowed for the specific objectives to be addressed. The specific objectives of this study were to i) gain an understanding of the long term space use patterns and site fidelity of sub-adult N. acutidens within degree of use of the St. Joseph Atoll lagoon and surrounding habitats, and ii) investigate their short term behavioural patterns in response to natural rhythmic cycles. The important role the atoll plays in the sub-adult stage of this species was demonstrated by the very low numbers of detections outside the atoll compared to the very large numbers of detections inside the atoll. Over the course of a year the tagged sharks showed a high degree of site fidelity to the atoll, with 79 – 100% of the individuals detected during each month and 50% being detected on a near daily basis. This result provides testimony of the nursery role of the atoll and importance of these habitats in the early life history for this species. Individual area use was generally found to be restricted to the eastern part of the atoll incorporating a portion of the central deep lagoon and a nearby adjacent area of the sand flats. The movements of sub-adult N. acutidens on and off of the shallow flats surrounding the central lagoon (likely for foraging) were found to be strongly influenced by the tidal height and the diel cycle. Tagged sharks were found to utilise the flats more regularly when the tidal height was greater than 90 cm and particularly more so during the night and early morning than during daylight times. The combined results of this study expose the vulnerability of this species to rapid local depletion. Similarly, the effectiveness of spatial management measures, such as no-take marine protected areas is highlighted as an efficient tool for the future conservation of this species. In the St. Joseph Atoll in particular, the use of a multi-levelled protection approach, where the eastern portion on the atoll is completely restricted while the remaining area is utilized for non-consumptive tourism activities, could be highly effective. Future research should investigate changes in area use throughout the ontogeny of this species as well improve the understanding of the breeding site fidelity and population size of reproductive females using the St. Joseph Atoll.
- Full Text:
Moving through the city : Cape Town's legacy of slavery and the performance of creolised carnival
- Authors: Nsele, Zamansele
- Date: 2012
- Subjects: Carnival -- South Africa -- Cape Town , Cape of Good Hope (South Africa) -- Economic conditions , Cape of Good Hope (South Africa) -- Social conditions , Cape Town -- Economic conditions , Cape Town -- Social conditions , Slavery -- South Africa -- Cape Town , Stereotypes (Social psychology -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2489 , http://hdl.handle.net/10962/d1012977
- Description: After South Africa entered into democracy in 1994, a mediation period of change was set in motion. It was an invitation for South Africans to imagine and envision themselves anew (Gqola 2010). Slave memory; a neglected past, that was previously silenced came to the fore and is currently in the process of being renegotiated in post-apartheid South Africa. In the light of this, I believe that the study of the Cape Minstrel Carnival which has its social roots in slavery lends itself for an insightful interpretation within an art historical framework. While institutionally the memory of slavery was officially marginalized: comparatively, on the streets of Cape Town, the community preserved it in elusive ways embodied in the procession of Carnival through the city. This thesis explores the imagery of creolisation, through an analysis of the Cape Minstrel Carnival. Zimitri Erasmus (Erasmus 200:14) defines creolisation as cultural production that happens under the specific conditions of slavery. Before I decode some of the motifs embedded in the imagery of creolisation, in chapter one I provide an in depth analysis; of the contextual conditions of which the practice of carnival originated. My analysis is informed extensively by post-colonial theories on race, identity, and creolisation. The route of the procession of carnival reveals an alternative and clandestine history of the city of Cape Town which I believe deserves focus. In chapter two I discuss its site specificity in relation to key urban sites, such as the District Six Museum, the Slave Lodge Museum and the Bo-Kaap Museum. This thesis explores the use of performance as a corporeal tool to demarcate the city. In the process of this analysis, a repertoire of movement becomes salient in the construction of creolised identities. In chapter three I discuss the motif of the “coon” as the most salient image of creolisation in the parade; I trace its iconographic roots to the performance of blackface minstrelsy that originated from the slave plantations of the United States of America. By unpacking the racist iconography bound up in the initial construction of the “coon”, it becomes clear that its derogative meaning was subverted when it was appropriated as a symbol of celebration into the New Year’s parades. As a result of its complicated history, some residents deride the parade as perpetuating racial stereotypes, by portraying “coloured” people as buffoons. Class snobbery has played a big part in the criticism. Therefore the procession of “the coons” or euphemistically the minstrels represents a cultural cringe for some and a festive celebration for others and both these sentiments coexist simultaneously. The Cape Town Minstrel Carnival can be interpreted on multiple shifting levels because it takes on an ambivalent and ambiguous position as far as meaning is concerned.
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- Authors: Nsele, Zamansele
- Date: 2012
- Subjects: Carnival -- South Africa -- Cape Town , Cape of Good Hope (South Africa) -- Economic conditions , Cape of Good Hope (South Africa) -- Social conditions , Cape Town -- Economic conditions , Cape Town -- Social conditions , Slavery -- South Africa -- Cape Town , Stereotypes (Social psychology -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2489 , http://hdl.handle.net/10962/d1012977
- Description: After South Africa entered into democracy in 1994, a mediation period of change was set in motion. It was an invitation for South Africans to imagine and envision themselves anew (Gqola 2010). Slave memory; a neglected past, that was previously silenced came to the fore and is currently in the process of being renegotiated in post-apartheid South Africa. In the light of this, I believe that the study of the Cape Minstrel Carnival which has its social roots in slavery lends itself for an insightful interpretation within an art historical framework. While institutionally the memory of slavery was officially marginalized: comparatively, on the streets of Cape Town, the community preserved it in elusive ways embodied in the procession of Carnival through the city. This thesis explores the imagery of creolisation, through an analysis of the Cape Minstrel Carnival. Zimitri Erasmus (Erasmus 200:14) defines creolisation as cultural production that happens under the specific conditions of slavery. Before I decode some of the motifs embedded in the imagery of creolisation, in chapter one I provide an in depth analysis; of the contextual conditions of which the practice of carnival originated. My analysis is informed extensively by post-colonial theories on race, identity, and creolisation. The route of the procession of carnival reveals an alternative and clandestine history of the city of Cape Town which I believe deserves focus. In chapter two I discuss its site specificity in relation to key urban sites, such as the District Six Museum, the Slave Lodge Museum and the Bo-Kaap Museum. This thesis explores the use of performance as a corporeal tool to demarcate the city. In the process of this analysis, a repertoire of movement becomes salient in the construction of creolised identities. In chapter three I discuss the motif of the “coon” as the most salient image of creolisation in the parade; I trace its iconographic roots to the performance of blackface minstrelsy that originated from the slave plantations of the United States of America. By unpacking the racist iconography bound up in the initial construction of the “coon”, it becomes clear that its derogative meaning was subverted when it was appropriated as a symbol of celebration into the New Year’s parades. As a result of its complicated history, some residents deride the parade as perpetuating racial stereotypes, by portraying “coloured” people as buffoons. Class snobbery has played a big part in the criticism. Therefore the procession of “the coons” or euphemistically the minstrels represents a cultural cringe for some and a festive celebration for others and both these sentiments coexist simultaneously. The Cape Town Minstrel Carnival can be interpreted on multiple shifting levels because it takes on an ambivalent and ambiguous position as far as meaning is concerned.
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Multi-instrument observations of ionospheric irregularities over South Africa
- Authors: Amabayo, Emirant Bertillas
- Date: 2012
- Subjects: Ionosphere -- Research , Sudden ionospheric disturbances , Ionospheric storms , Solar activity , Sunspots
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5476 , http://hdl.handle.net/10962/d1005261 , Ionosphere -- Research , Sudden ionospheric disturbances , Ionospheric storms , Solar activity , Sunspots
- Description: The occurrence of mid-latitude spread F (SF) over South Africa has not been extensively studied since the installation of the DPS-4 digisondes at Madimbo (30.88◦E, 22.38◦S), Grahamstown (33.32◦S, 26.50◦E) and Louisvale (28.51◦S, 21.24◦E). This study is intended to quantify the probability of the occurrence of F region disturbances associated with ionospheric spread F (SF) and L-band scintillation over South Africa. This study used available ionosonde data for 8 years (2000-2008) from the three South African stations. The SF events were identified manually on ionograms and grouped for further statistical analysis into frequency SF (FSF), range SF (RSF) and mixed SF (MSF). The results show that the diurnal pattern of SF occurrence peaks strongly between 23:00 and 00:00 UT. This pattern is true for all seasons and types of SF at Madimbo and Grahamstown during 2001 and 2005, except for RSF which had peaks during autumn and spring during 2001 at Madimbo. The probability of both MSF and FSF tends to increase with decreasing sunspot number (SSN), with a peak in 2005 (a moderate solar activity period). The seasonal peaks of MSF and FSF are more frequent during winter months at both Madimbo and Grahamstown. In this study SF was evident in ∼ 0.03% and ∼ 0.06% of the available ionograms at Madimbo and Grahamstown respectively during the eight year period. The presence of ionospheric irregularities associated with SF and scintillation was investigated using data from selected Global Positioning System (GPS) receiver stations distributed across South Africa. The results, based on GPS total electron content (TEC) and ionosonde measurements, show that SF over this region can most likely be attributed to travelling ionospheric disturbances (TIDs), caused by gravity waves (GWs) and neutral wind composition changes. The GWs were mostly associated with geomagnetic storms and sub-storms that occurred during periods of high and moderate solar activity (2001-2005). SF occurrence during the low solar activity period (2006-2008)can probably be attributed to neutral wind composition changes.
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- Authors: Amabayo, Emirant Bertillas
- Date: 2012
- Subjects: Ionosphere -- Research , Sudden ionospheric disturbances , Ionospheric storms , Solar activity , Sunspots
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5476 , http://hdl.handle.net/10962/d1005261 , Ionosphere -- Research , Sudden ionospheric disturbances , Ionospheric storms , Solar activity , Sunspots
- Description: The occurrence of mid-latitude spread F (SF) over South Africa has not been extensively studied since the installation of the DPS-4 digisondes at Madimbo (30.88◦E, 22.38◦S), Grahamstown (33.32◦S, 26.50◦E) and Louisvale (28.51◦S, 21.24◦E). This study is intended to quantify the probability of the occurrence of F region disturbances associated with ionospheric spread F (SF) and L-band scintillation over South Africa. This study used available ionosonde data for 8 years (2000-2008) from the three South African stations. The SF events were identified manually on ionograms and grouped for further statistical analysis into frequency SF (FSF), range SF (RSF) and mixed SF (MSF). The results show that the diurnal pattern of SF occurrence peaks strongly between 23:00 and 00:00 UT. This pattern is true for all seasons and types of SF at Madimbo and Grahamstown during 2001 and 2005, except for RSF which had peaks during autumn and spring during 2001 at Madimbo. The probability of both MSF and FSF tends to increase with decreasing sunspot number (SSN), with a peak in 2005 (a moderate solar activity period). The seasonal peaks of MSF and FSF are more frequent during winter months at both Madimbo and Grahamstown. In this study SF was evident in ∼ 0.03% and ∼ 0.06% of the available ionograms at Madimbo and Grahamstown respectively during the eight year period. The presence of ionospheric irregularities associated with SF and scintillation was investigated using data from selected Global Positioning System (GPS) receiver stations distributed across South Africa. The results, based on GPS total electron content (TEC) and ionosonde measurements, show that SF over this region can most likely be attributed to travelling ionospheric disturbances (TIDs), caused by gravity waves (GWs) and neutral wind composition changes. The GWs were mostly associated with geomagnetic storms and sub-storms that occurred during periods of high and moderate solar activity (2001-2005). SF occurrence during the low solar activity period (2006-2008)can probably be attributed to neutral wind composition changes.
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Municipal commonage: an undervalued national resource
- Gambiza, James, Shackleton, Charlie M, Davenport, N I, Atkinson, D, Hoffman, Michael T, Martens, C, Puttick, J, De Groot, W
- Authors: Gambiza, James , Shackleton, Charlie M , Davenport, N I , Atkinson, D , Hoffman, Michael T , Martens, C , Puttick, J , De Groot, W
- Date: 2012
- Subjects: Land reform -- South Africa Land use -- South Africa South Africa -- Social conditions
- Language: English
- Type: text
- Identifier: http://hdl.handle.net/10962/49969 , vital:25947
- Description: This policy brief will highlight the importance of municipal commonage in enhancing livelihoods of poor urban families. This will be followed by an overview of the role of municipal commonage in the land reform programme. Current challenges in securing benefits from municipal commonages are then outlined. Policy considerations to optimise benefits from commonages conclude the policy brief.
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- Authors: Gambiza, James , Shackleton, Charlie M , Davenport, N I , Atkinson, D , Hoffman, Michael T , Martens, C , Puttick, J , De Groot, W
- Date: 2012
- Subjects: Land reform -- South Africa Land use -- South Africa South Africa -- Social conditions
- Language: English
- Type: text
- Identifier: http://hdl.handle.net/10962/49969 , vital:25947
- Description: This policy brief will highlight the importance of municipal commonage in enhancing livelihoods of poor urban families. This will be followed by an overview of the role of municipal commonage in the land reform programme. Current challenges in securing benefits from municipal commonages are then outlined. Policy considerations to optimise benefits from commonages conclude the policy brief.
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Musculoskeletal and perceptual responses of batsmen comparing high- and moderate-volume sprints between the wickets
- Authors: Sheppard, Bronwyn Jane
- Date: 2012
- Subjects: Cricket -- Batting -- Physiological aspects , Cricket injuries , Musculoskeletal system -- Wounds and injuries
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5171 , http://hdl.handle.net/10962/d1016366
- Description: Background: Literature has associated repeated eccentric muscle actions with increased muscle damage of the muscles involved. Eccentric actions are typical in sports which are ‘stop-start’ in nature requiring rapid acceleration and deceleration, typical of a batting activity in cricket. Ultra-structural damage of the skeletal muscle as a consequence of repeated decelerating activities is associated with performance decrements, particularly muscle strength and sprinting speeds. This suggests that eccentric strength decrements may provide an indication for the development of muscle strain injuries during these activities. Despite these findings, limited research has identified the specific musculoskeletal demands placed on cricket batsmen, particularly with reference to various match intensities. Objective: The present study, therefore, sought to determine the specific musculoskeletal, physiological and perceptual demands placed on specialised batsmen during two work bouts of different intensities; one representing a highintensity work bout and the other a moderate-intensity work bout. The dependent variables of interest were muscle activation, isokinetic strength changes, heart rate, ‘central’ and ‘local’ ratings of perceived exertion (RPE), body discomfort and performance. Methods: The two experimental conditions, representative of a high- (HVR) and moderate-volume running (MVR) batting protocol, required players to perform a simulated batting work bout of either twelve or six runs an over, within a laboratory setting. Selected physiological, perceptual and performance measures were collected at specific time intervals throughout the work bout while the biophysical measures were collected prior to, and following both protocols. Results: Of the variables measured, heart rate, ‘central’ and ‘local’ RPE values were observed to increase significantly (p<0.05) over time. This increase was greater as a consequence of the HVR in comparison to the MVR. No change in sprint times was documented during the MVR, in contrast, significant (p<0.05) increases over time were observed during the HVR, further highlighting the elevated demands associated with this condition. In addition, an ‘end spurt’ was observed particularly following the HVR condition, suggesting athletes were conserving themselves through the adoption of a pacing strategy. Reductions in biceps femoris and semitendinosus muscle activation levels were observed following the HVR. This was further supported by the significantly greater levels of semitendinosus activation following the MVR when compared to the HVR. Peak concentric and eccentric knee extensor (EXT) (-17.17% and -16.07% respectively) and eccentric flexor (FLEX) (- 17.49%) values decreased significantly (p<0.05) following the HVR at 60°.s-1. In addition, concentric and eccentric total work produced by the flexors and eccentric extensors resulted in significantly (p<0.05) lower values due to the HVR. Conclusion: The intermittent high-volume batting work bout elicited elevated mean heart rates, perceived ratings of cardiovascular and muscular effort and sprint times. Furthermore, hamstring activation levels and muscle strength, particularly concentric strength of the dominant lower limb were negatively affected by the HVR condition. These results suggest elevated demands were placed on the hamstring musculature as a consequence of the HVR condition, indicating a greater degree of musculoskeletal strain and increased injury risk associated with running between the wickets at this intensity, representative of an aggressive batting scenario.
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- Authors: Sheppard, Bronwyn Jane
- Date: 2012
- Subjects: Cricket -- Batting -- Physiological aspects , Cricket injuries , Musculoskeletal system -- Wounds and injuries
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5171 , http://hdl.handle.net/10962/d1016366
- Description: Background: Literature has associated repeated eccentric muscle actions with increased muscle damage of the muscles involved. Eccentric actions are typical in sports which are ‘stop-start’ in nature requiring rapid acceleration and deceleration, typical of a batting activity in cricket. Ultra-structural damage of the skeletal muscle as a consequence of repeated decelerating activities is associated with performance decrements, particularly muscle strength and sprinting speeds. This suggests that eccentric strength decrements may provide an indication for the development of muscle strain injuries during these activities. Despite these findings, limited research has identified the specific musculoskeletal demands placed on cricket batsmen, particularly with reference to various match intensities. Objective: The present study, therefore, sought to determine the specific musculoskeletal, physiological and perceptual demands placed on specialised batsmen during two work bouts of different intensities; one representing a highintensity work bout and the other a moderate-intensity work bout. The dependent variables of interest were muscle activation, isokinetic strength changes, heart rate, ‘central’ and ‘local’ ratings of perceived exertion (RPE), body discomfort and performance. Methods: The two experimental conditions, representative of a high- (HVR) and moderate-volume running (MVR) batting protocol, required players to perform a simulated batting work bout of either twelve or six runs an over, within a laboratory setting. Selected physiological, perceptual and performance measures were collected at specific time intervals throughout the work bout while the biophysical measures were collected prior to, and following both protocols. Results: Of the variables measured, heart rate, ‘central’ and ‘local’ RPE values were observed to increase significantly (p<0.05) over time. This increase was greater as a consequence of the HVR in comparison to the MVR. No change in sprint times was documented during the MVR, in contrast, significant (p<0.05) increases over time were observed during the HVR, further highlighting the elevated demands associated with this condition. In addition, an ‘end spurt’ was observed particularly following the HVR condition, suggesting athletes were conserving themselves through the adoption of a pacing strategy. Reductions in biceps femoris and semitendinosus muscle activation levels were observed following the HVR. This was further supported by the significantly greater levels of semitendinosus activation following the MVR when compared to the HVR. Peak concentric and eccentric knee extensor (EXT) (-17.17% and -16.07% respectively) and eccentric flexor (FLEX) (- 17.49%) values decreased significantly (p<0.05) following the HVR at 60°.s-1. In addition, concentric and eccentric total work produced by the flexors and eccentric extensors resulted in significantly (p<0.05) lower values due to the HVR. Conclusion: The intermittent high-volume batting work bout elicited elevated mean heart rates, perceived ratings of cardiovascular and muscular effort and sprint times. Furthermore, hamstring activation levels and muscle strength, particularly concentric strength of the dominant lower limb were negatively affected by the HVR condition. These results suggest elevated demands were placed on the hamstring musculature as a consequence of the HVR condition, indicating a greater degree of musculoskeletal strain and increased injury risk associated with running between the wickets at this intensity, representative of an aggressive batting scenario.
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Nanocomposites of nickel phthalocyanines as electrocatalysts for the oxidation of chlorophenols an experimental and theoretical approach
- Authors: Khene, Mielie Samson
- Date: 2012
- Subjects: Nanocomposites (Materials) -- Research Phthalocyanines -- Research Chlorophenols -- Research Electrocatalysis -- Research
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4326 , http://hdl.handle.net/10962/d1004986
- Description: In this work the interaction between peripherally (b) substituted nickel tetrahydroxyphthalocyanines (b-NiPc(OH)4 and poly-b-Ni(O)Pc(OH)4) with 4-chlorophenol is theoretically rationalized by performing calculations at the B3LYP/6-31G(d) level. Density functional theory (DFT) and molecular orbital theory are used to calculate the condensed Fukui function for phthalocyanine derivatives and 4-chlorophenol, in order to determine the reactive sites involved when 4-chlorophenol is oxidized, and to compare theoretically predicted reactivity to experimentally determined electrocatalytic activity. Electrocatalytic activities of adsorbed NiPc derivatives: OPGE-α-NiPc(OH)8 (OPGE = ordinary pyrolytic graphite electrode, α = nonperipheral substitution), OPGE-a-NiPc(OH)4 and OPGE-β- NiPc(OH)4 are compared with those of the polymerized counterparts: OPGE-poly-α-Ni(O)Pc(OH)8, OPGE poly-a-NiPc(OH)4 and OPGE-poly-β-NiPc(OH)4, respectively. β-NiPc(NH2)4, β-NiPc(OH)4, α-NiPc(OH)4, α-NiPc(OH)8, α-NiPc(C10H21)8 are adsorbed on singled walled carbon nanotube (SWCNT) and β-NiPc(NH2)4-SWCNT (linked to SWCNT), are used to modify glassy carbon electrode (GCE) and employed for the electro oxidation of chlorophenols. The β-NiPc(NH2)4-SWCNT gave the best current response for the oxidation of 4-chlorophenol. α-NiPc(OH)8-SWCNT and α-NiPc(C10H21)8 gave the best resistance to electrode fouling due to oxidation by product of 4-chlorophenol. The synthesis of conjugates of cadmium telluride quantum dots (CdTe-QDs) capped with thioglycolic acid and peripherally substituted nickel tetraamino phthalocyanine (β-NiPc(NH2)4) complex were also employed for the oxidation of chlorophenols. Separation of one of the diastereomers of 1, 2-subnaphthalocyanine (SubNPc) was achieved as well as separation of the enantiomers. The absorption and magnetic circular dichroism spectra, together with theoretical calculations, reveal rather small variations in the frontier molecular orbitals of the SubNPc compared to conventional subphthalocyanine (SubPc), except for the destabilization of the highest occupied molecular orbital (HOMO), which results in a characteristic absorption in the Soret band region. The chirality of SubNPc, including the cyclic dichroism (CD) signs and intensities, are discussed in detail.
- Full Text:
- Authors: Khene, Mielie Samson
- Date: 2012
- Subjects: Nanocomposites (Materials) -- Research Phthalocyanines -- Research Chlorophenols -- Research Electrocatalysis -- Research
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4326 , http://hdl.handle.net/10962/d1004986
- Description: In this work the interaction between peripherally (b) substituted nickel tetrahydroxyphthalocyanines (b-NiPc(OH)4 and poly-b-Ni(O)Pc(OH)4) with 4-chlorophenol is theoretically rationalized by performing calculations at the B3LYP/6-31G(d) level. Density functional theory (DFT) and molecular orbital theory are used to calculate the condensed Fukui function for phthalocyanine derivatives and 4-chlorophenol, in order to determine the reactive sites involved when 4-chlorophenol is oxidized, and to compare theoretically predicted reactivity to experimentally determined electrocatalytic activity. Electrocatalytic activities of adsorbed NiPc derivatives: OPGE-α-NiPc(OH)8 (OPGE = ordinary pyrolytic graphite electrode, α = nonperipheral substitution), OPGE-a-NiPc(OH)4 and OPGE-β- NiPc(OH)4 are compared with those of the polymerized counterparts: OPGE-poly-α-Ni(O)Pc(OH)8, OPGE poly-a-NiPc(OH)4 and OPGE-poly-β-NiPc(OH)4, respectively. β-NiPc(NH2)4, β-NiPc(OH)4, α-NiPc(OH)4, α-NiPc(OH)8, α-NiPc(C10H21)8 are adsorbed on singled walled carbon nanotube (SWCNT) and β-NiPc(NH2)4-SWCNT (linked to SWCNT), are used to modify glassy carbon electrode (GCE) and employed for the electro oxidation of chlorophenols. The β-NiPc(NH2)4-SWCNT gave the best current response for the oxidation of 4-chlorophenol. α-NiPc(OH)8-SWCNT and α-NiPc(C10H21)8 gave the best resistance to electrode fouling due to oxidation by product of 4-chlorophenol. The synthesis of conjugates of cadmium telluride quantum dots (CdTe-QDs) capped with thioglycolic acid and peripherally substituted nickel tetraamino phthalocyanine (β-NiPc(NH2)4) complex were also employed for the oxidation of chlorophenols. Separation of one of the diastereomers of 1, 2-subnaphthalocyanine (SubNPc) was achieved as well as separation of the enantiomers. The absorption and magnetic circular dichroism spectra, together with theoretical calculations, reveal rather small variations in the frontier molecular orbitals of the SubNPc compared to conventional subphthalocyanine (SubPc), except for the destabilization of the highest occupied molecular orbital (HOMO), which results in a characteristic absorption in the Soret band region. The chirality of SubNPc, including the cyclic dichroism (CD) signs and intensities, are discussed in detail.
- Full Text: