Biochemical characterisation of Pfj2, a Plasmodium falciparum heat shock protein 40 chaperone potentially involved in protein quality control in the endoplasmic reticulum
- Authors: Afolayan, Omolola Folasade
- Date: 2013
- Subjects: Plasmodium falciparum Endoplasmic reticulum Heat shock proteins Malaria , Mosquito-borne infectious disease
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3883 , http://hdl.handle.net/10962/d1001617
- Description: Plasmodium falciparum is a protozoan parasite that causes a severe form of malaria, a mosquito-borne infectious disease in humans. P. falciparum encodes a number of proteins to facilitate its life-cycle, including a type II heat shock protein 40 (Hsp40), Pfj2. Pfj2 shows a degree of homology to human ERdj5, a resident protein of the endoplasmic reticulum (ER) that promotes protein quality control by facilitating the degradation of misfolded proteins. The overall aim of this study was to further understand the function of Pfj2 in the P. falciparum cell by characterising it biochemically. A bioinformatic analysis of Pfj2 was carried out to enable the identification of a potential ER signal sequence and cleavage site. Furthermore, an analysis of Pfj2 protein sequence was performed to compare domain similarities and identities with typical type II Hsp40s namely, human ERdj5, S. cerevisiae Sis1, human Hsj1a and human DnaJB4. The method used included the insertion of the codon-optimised coding sequence for the processed ER form of Pfj2 into the prokaryotic expression vector, pQE30, to enable overproduction of a histidine-tagged protein. A 62 kDa His₆-Pfj2 was successfully expressed in Escherichia coli and purified using denaturing nickel affinity chromatography. ATPase assays were performed to determine the ability of His₆- Pfj2 to stimulate the chaperone activity of the ER Hsp70, also called immunoglobulin binding protein (BiP). Initial studies were conducted on readily available mammalian His₆-BiP as a control, which was shown to have an intrinsic activity of 12.07±3.92 nmolPi/min/mg. His₆- Pfj2 did not stimulate the ATPase activity of mammalian His₆-BiP, suggesting that it either could not act as a co-chaperone of mammalian His₆-BiP (specificity), or it required a misfolded substrate in the system. Therefore, ongoing studies are addressing the interaction of Pfj2 and misfolded substrates with P. falciparum BiP. The results of these studies will further our understanding of a poorly-studied parasite chaperone that represents a potential drug target for development of novel strategies for the control of a serious human disease
- Full Text:
- Date Issued: 2013
- Authors: Afolayan, Omolola Folasade
- Date: 2013
- Subjects: Plasmodium falciparum Endoplasmic reticulum Heat shock proteins Malaria , Mosquito-borne infectious disease
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3883 , http://hdl.handle.net/10962/d1001617
- Description: Plasmodium falciparum is a protozoan parasite that causes a severe form of malaria, a mosquito-borne infectious disease in humans. P. falciparum encodes a number of proteins to facilitate its life-cycle, including a type II heat shock protein 40 (Hsp40), Pfj2. Pfj2 shows a degree of homology to human ERdj5, a resident protein of the endoplasmic reticulum (ER) that promotes protein quality control by facilitating the degradation of misfolded proteins. The overall aim of this study was to further understand the function of Pfj2 in the P. falciparum cell by characterising it biochemically. A bioinformatic analysis of Pfj2 was carried out to enable the identification of a potential ER signal sequence and cleavage site. Furthermore, an analysis of Pfj2 protein sequence was performed to compare domain similarities and identities with typical type II Hsp40s namely, human ERdj5, S. cerevisiae Sis1, human Hsj1a and human DnaJB4. The method used included the insertion of the codon-optimised coding sequence for the processed ER form of Pfj2 into the prokaryotic expression vector, pQE30, to enable overproduction of a histidine-tagged protein. A 62 kDa His₆-Pfj2 was successfully expressed in Escherichia coli and purified using denaturing nickel affinity chromatography. ATPase assays were performed to determine the ability of His₆- Pfj2 to stimulate the chaperone activity of the ER Hsp70, also called immunoglobulin binding protein (BiP). Initial studies were conducted on readily available mammalian His₆-BiP as a control, which was shown to have an intrinsic activity of 12.07±3.92 nmolPi/min/mg. His₆- Pfj2 did not stimulate the ATPase activity of mammalian His₆-BiP, suggesting that it either could not act as a co-chaperone of mammalian His₆-BiP (specificity), or it required a misfolded substrate in the system. Therefore, ongoing studies are addressing the interaction of Pfj2 and misfolded substrates with P. falciparum BiP. The results of these studies will further our understanding of a poorly-studied parasite chaperone that represents a potential drug target for development of novel strategies for the control of a serious human disease
- Full Text:
- Date Issued: 2013
Statistical model for risk diversification in renewable energy
- Authors: Ahame, Edmund
- Date: 2013
- Subjects: Renewable energy sources -- Statistics , Diversification in industry
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10564 , http://hdl.handle.net/10948/d1008399 , Renewable energy sources -- Statistics , Diversification in industry
- Description: The growth of the industry and population of South Africa urges to seek new sources of electric power, hence the need to look at alternative power sources. Power output from some renewable energy sources is highly volatile. For instance power output from wind turbines or photovoltaic solar panels fluctuates between zero and the maximum rated power out. To optimize the overall power output a model was designed to determine the best trade-off between production from two or more renewable energy sources putting emphasis on wind and solar. Different measures of risk, such as coefficient of variation (CV) and value at risk (VAR), were used to determine the best hybrid renewable energy system (HRES) configuration. Depending on the investors’ expected returns (demand) and risk averseness, they will be able to use the model to choose the best configuration that suites their needs. In general it was found that investing in a diversified HRES is better than investing in individual power sources.
- Full Text:
- Date Issued: 2013
- Authors: Ahame, Edmund
- Date: 2013
- Subjects: Renewable energy sources -- Statistics , Diversification in industry
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10564 , http://hdl.handle.net/10948/d1008399 , Renewable energy sources -- Statistics , Diversification in industry
- Description: The growth of the industry and population of South Africa urges to seek new sources of electric power, hence the need to look at alternative power sources. Power output from some renewable energy sources is highly volatile. For instance power output from wind turbines or photovoltaic solar panels fluctuates between zero and the maximum rated power out. To optimize the overall power output a model was designed to determine the best trade-off between production from two or more renewable energy sources putting emphasis on wind and solar. Different measures of risk, such as coefficient of variation (CV) and value at risk (VAR), were used to determine the best hybrid renewable energy system (HRES) configuration. Depending on the investors’ expected returns (demand) and risk averseness, they will be able to use the model to choose the best configuration that suites their needs. In general it was found that investing in a diversified HRES is better than investing in individual power sources.
- Full Text:
- Date Issued: 2013
Information technology audits in South African higher education institutions
- Authors: Angus, Lynne
- Date: 2013 , 2013-09-11
- Subjects: Electronic data processing -- Auditing , Delphi method , Education, Higher -- Computer networks -- Security measures , Information technology -- Security measures , COBIT (Information technology management standard) , IT infrastructure library , International Organization for Standardization
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4615 , http://hdl.handle.net/10962/d1006023 , Electronic data processing -- Auditing , Delphi method , Education, Higher -- Computer networks -- Security measures , Information technology -- Security measures , COBIT (Information technology management standard) , IT infrastructure library , International Organization for Standardization
- Description: The use of technology for competitive advantage has become a necessity, not only for corporate organisations, but for higher education institutions (HEIs) as well. Consequently, corporate organisations and HEIs alike must be equipped to protect against the pervasive nature of technology. To do this, they implement controls and undergo audits to ensure these controls are implemented correctly. Although HEIs are a different kind of entity to corporate organisations, HEI information technology (IT) audits are based on the same criteria as those for corporate organisations. The primary aim of this research, therefore, was to develop a set of IT control criteria that are relevant to be tested in IT audits for South African HEIs. The research method used was the Delphi technique. Data was collected, analysed, and used as feedback on which to progress to the next round of data collection. Two lists were obtained: a list of the top IT controls relevant to be tested at any organisation, and a list of the top IT controls relevant to be tested at a South African HEI. Comparison of the two lists shows that although there are some differences in the ranking of criteria used to audit corporate organisations as opposed to HEIs, the final two lists of criteria do not differ significantly. Therefore, it was shown that the same broad IT controls are required to be tested in an IT audit for a South African HEI. However, this research suggests that the risk weighting put on particular IT controls should possibly differ for HEIs, as HEIs face differing IT risks. If further studies can be established which cater for more specific controls, then the combined effect of this study and future ones will be a valuable contribution to knowledge for IT audits in a South African higher education context.
- Full Text:
- Date Issued: 2013
- Authors: Angus, Lynne
- Date: 2013 , 2013-09-11
- Subjects: Electronic data processing -- Auditing , Delphi method , Education, Higher -- Computer networks -- Security measures , Information technology -- Security measures , COBIT (Information technology management standard) , IT infrastructure library , International Organization for Standardization
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4615 , http://hdl.handle.net/10962/d1006023 , Electronic data processing -- Auditing , Delphi method , Education, Higher -- Computer networks -- Security measures , Information technology -- Security measures , COBIT (Information technology management standard) , IT infrastructure library , International Organization for Standardization
- Description: The use of technology for competitive advantage has become a necessity, not only for corporate organisations, but for higher education institutions (HEIs) as well. Consequently, corporate organisations and HEIs alike must be equipped to protect against the pervasive nature of technology. To do this, they implement controls and undergo audits to ensure these controls are implemented correctly. Although HEIs are a different kind of entity to corporate organisations, HEI information technology (IT) audits are based on the same criteria as those for corporate organisations. The primary aim of this research, therefore, was to develop a set of IT control criteria that are relevant to be tested in IT audits for South African HEIs. The research method used was the Delphi technique. Data was collected, analysed, and used as feedback on which to progress to the next round of data collection. Two lists were obtained: a list of the top IT controls relevant to be tested at any organisation, and a list of the top IT controls relevant to be tested at a South African HEI. Comparison of the two lists shows that although there are some differences in the ranking of criteria used to audit corporate organisations as opposed to HEIs, the final two lists of criteria do not differ significantly. Therefore, it was shown that the same broad IT controls are required to be tested in an IT audit for a South African HEI. However, this research suggests that the risk weighting put on particular IT controls should possibly differ for HEIs, as HEIs face differing IT risks. If further studies can be established which cater for more specific controls, then the combined effect of this study and future ones will be a valuable contribution to knowledge for IT audits in a South African higher education context.
- Full Text:
- Date Issued: 2013
Evaluating the training and supply of artisans in the South African construction industry
- Authors: Anugwo, Iruka Chijindu
- Date: 2013
- Subjects: Skilled labor -- South Africa , Skilled labor -- Training -- South Africa , Artisans -- South Africa , Construction industry -- Employees --Training of -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:9672 , http://hdl.handle.net/10948/d1020042
- Description: The study focused on identifying ways in which to improve and increase the quality of training and supply of skilled artisans in the South African construction industry (especially in Gauteng Province) in terms of short and medium term approach. The construction industry is facing a severe skills crisis and this has made it so difficult to recruit competent artisans. This may be as a result of artisans being regarded as the lowest in the hierarchy of construction worker positions. In addition, these artisans warrant less training than those at executive- and managerial levels. This negative attitude towards artisans training has plunged the industry into crisis. Those involved find it difficult to successfully complete projects on time, within budgeted project costs and within the required quality specification due to lack of competent artisans. However, serious attention is required towards enhancing artisan training in the industry. It is imperative and vital to ensure the survival of the industry. The cardinal aim of this research project was to demonstrate a guiding solution towards the skills crisis. Thus, in order to initiate a tremendous change in the skills profile, certain measures are required, e.g. expanding training institutions to local regions; upgrading and aligning facilities in the training institutions to industrial demands and the dissemination of vital information that will ensure the attractiveness of the industry. This will safeguard the construction organisations operating in Gauteng Province. Although the industry is a major player in the economy, there is need for special attention in order to ensure sustainable growth and economic development. The Government, construction industry stakeholders and training institutions personnel should collaborate to salvage the skills crisis by strategically developing programmes (in accordance with industrial requirements) that will benefit prospective learners. The researcher adopted the descriptive- and analytical survey method, which entailed the use of questionnaires and a review of the related literature for gathering relevant data. The methodology used in the research was the quantitative data analysis. The feature findings of the research concluded that the skilled artisans profile is insufficient to meet industry demands. In addition, training institutions and primary- and secondary educational systems are characterized by ninadequacy and incompetency to produce skilled artisans. The situation is exacerbated by the lack of harmonization between the training institutions and the vindustry’s requirements. Furthermore, the majority of the respondents acknowledged that training of their workers is an important aspect to them, but few of these organisations indicated that they make use of formal training institutions such as FET colleges. The recommendation include that all the stakeholders in the construction industry should form collaborations to strategically develop programmes that would upgrade the existing training institutions. This could be done through provision of adequate funds and resources and the dissemination of vital information that is capable to promote the attractiveness of the industry image.
- Full Text:
- Date Issued: 2013
- Authors: Anugwo, Iruka Chijindu
- Date: 2013
- Subjects: Skilled labor -- South Africa , Skilled labor -- Training -- South Africa , Artisans -- South Africa , Construction industry -- Employees --Training of -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:9672 , http://hdl.handle.net/10948/d1020042
- Description: The study focused on identifying ways in which to improve and increase the quality of training and supply of skilled artisans in the South African construction industry (especially in Gauteng Province) in terms of short and medium term approach. The construction industry is facing a severe skills crisis and this has made it so difficult to recruit competent artisans. This may be as a result of artisans being regarded as the lowest in the hierarchy of construction worker positions. In addition, these artisans warrant less training than those at executive- and managerial levels. This negative attitude towards artisans training has plunged the industry into crisis. Those involved find it difficult to successfully complete projects on time, within budgeted project costs and within the required quality specification due to lack of competent artisans. However, serious attention is required towards enhancing artisan training in the industry. It is imperative and vital to ensure the survival of the industry. The cardinal aim of this research project was to demonstrate a guiding solution towards the skills crisis. Thus, in order to initiate a tremendous change in the skills profile, certain measures are required, e.g. expanding training institutions to local regions; upgrading and aligning facilities in the training institutions to industrial demands and the dissemination of vital information that will ensure the attractiveness of the industry. This will safeguard the construction organisations operating in Gauteng Province. Although the industry is a major player in the economy, there is need for special attention in order to ensure sustainable growth and economic development. The Government, construction industry stakeholders and training institutions personnel should collaborate to salvage the skills crisis by strategically developing programmes (in accordance with industrial requirements) that will benefit prospective learners. The researcher adopted the descriptive- and analytical survey method, which entailed the use of questionnaires and a review of the related literature for gathering relevant data. The methodology used in the research was the quantitative data analysis. The feature findings of the research concluded that the skilled artisans profile is insufficient to meet industry demands. In addition, training institutions and primary- and secondary educational systems are characterized by ninadequacy and incompetency to produce skilled artisans. The situation is exacerbated by the lack of harmonization between the training institutions and the vindustry’s requirements. Furthermore, the majority of the respondents acknowledged that training of their workers is an important aspect to them, but few of these organisations indicated that they make use of formal training institutions such as FET colleges. The recommendation include that all the stakeholders in the construction industry should form collaborations to strategically develop programmes that would upgrade the existing training institutions. This could be done through provision of adequate funds and resources and the dissemination of vital information that is capable to promote the attractiveness of the industry image.
- Full Text:
- Date Issued: 2013
Changes in muscle recruitment, functional strength and ratings of perceived effort during an 8-over bowling spell: impact on performance
- Authors: Barford, Gareth Charles
- Date: 2013
- Subjects: Cricket -- Bowling , Sports -- Physiological aspects , Muscles -- Wounds and injuries , Fatigue , Physical fitness , Cricket injuries
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5102 , http://hdl.handle.net/10962/d1003926 , Cricket -- Bowling , Sports -- Physiological aspects , Muscles -- Wounds and injuries , Fatigue , Physical fitness , Cricket injuries
- Description: Background: The musculoskeletal demands placed on the lower limb musculature of fast bowlers over time have not received much attention. In particular, measures of muscle recruitment changes have, to the author’s knowledge, not been considered. Objective: The present study, therefore sought to establish any associations between an eight over, simulated fast bowling spell, and muscle activation patterns, power output, perceptual demands, and changes in performance. This will enable improvements in the development of training programmes. Methods: Players’ were required to attend two sessions in total. The purpose of the initial session was to collect specific demographic, anthropometric and physiological data and injury history information from each player. In addition, this first session allowed for habituation with the treadmill, the jump meter and all other equipment involved in experimentation. The second testing session involved electrode attachment sites being identified on player’s dominant leg. The areas were then shaved, wiped with an alcohol swab and left to dry, to ensure good connectivity. Pre- and post- measures of muscle activity and functional strength of the lower limbs were recorded in the Department of Human Kinetics and Ergonomics. The protocol took place at the Kingswood High Performance Centre, which is in close proximity to the initial testing site. The protocol involved players bowling eight overs (48 balls). During the protocol, accuracy, ball release speed and perceptual measures were recorded at the end of each over. After the protocol, players were driven back to the Human Kinetics and Ergonomics Department where post-testing measures were completed. The dependable variables of interest were muscle activation, functional strength of the lower limbs, ‘local’ ratings of perceived exertion (RPE), body discomfort, accuracy, and ball release speed. Results: For all muscles it was shown that, as the speed increased so did the muscle activity in players’ lower limbs. There were no significant changes in muscle activity preversus post-protocol. There was however, a general trend of decreasing muscle activity post protocol at higher testing speeds. There were significant (p<0.05) decreases in peak power following the simulated eight over bowling spell. ‘Local’ RPE displayed a significant (P<0.05) increase with each additional over and were observed to reach the ‘heavy’ category. The players’ highest discomfort area was in the lower back, with 13 players perceiving discomfort in this region following the eight over spell. The shoulder and chest were another two areas player’s indicated discomfort with eight players selecting the dominant shoulder. Seven players complained of the dominant side pectoral muscle, leading foot and dominant latissimus dorsi muscle being uncomfortable. Interestingly, the dominant pectoral showed the highest body discomfort ratings amongst players. There were no significant changes in accuracy between overs although there were large interindividual differences in accuracy points between players. The decrease in ball release speed observed during over seven was shown to be significantly (p<0.05) lower than overs one to four. Conclusion: The power output and perceived strain results of the players, appears to indicate the presence of fatigue in players. However, the results are not conclusive, as the fatigue was not shown in muscle recruitment patterns, as well as the body discomfort ratings. There was a non-significant trend observed in the lower limb muscle activation decreasing at higher speeds. Players were able to maintain accuracy. However, the significantly lower ball release speed observed during over seven showed players performance decreasing.
- Full Text:
- Date Issued: 2013
- Authors: Barford, Gareth Charles
- Date: 2013
- Subjects: Cricket -- Bowling , Sports -- Physiological aspects , Muscles -- Wounds and injuries , Fatigue , Physical fitness , Cricket injuries
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5102 , http://hdl.handle.net/10962/d1003926 , Cricket -- Bowling , Sports -- Physiological aspects , Muscles -- Wounds and injuries , Fatigue , Physical fitness , Cricket injuries
- Description: Background: The musculoskeletal demands placed on the lower limb musculature of fast bowlers over time have not received much attention. In particular, measures of muscle recruitment changes have, to the author’s knowledge, not been considered. Objective: The present study, therefore sought to establish any associations between an eight over, simulated fast bowling spell, and muscle activation patterns, power output, perceptual demands, and changes in performance. This will enable improvements in the development of training programmes. Methods: Players’ were required to attend two sessions in total. The purpose of the initial session was to collect specific demographic, anthropometric and physiological data and injury history information from each player. In addition, this first session allowed for habituation with the treadmill, the jump meter and all other equipment involved in experimentation. The second testing session involved electrode attachment sites being identified on player’s dominant leg. The areas were then shaved, wiped with an alcohol swab and left to dry, to ensure good connectivity. Pre- and post- measures of muscle activity and functional strength of the lower limbs were recorded in the Department of Human Kinetics and Ergonomics. The protocol took place at the Kingswood High Performance Centre, which is in close proximity to the initial testing site. The protocol involved players bowling eight overs (48 balls). During the protocol, accuracy, ball release speed and perceptual measures were recorded at the end of each over. After the protocol, players were driven back to the Human Kinetics and Ergonomics Department where post-testing measures were completed. The dependable variables of interest were muscle activation, functional strength of the lower limbs, ‘local’ ratings of perceived exertion (RPE), body discomfort, accuracy, and ball release speed. Results: For all muscles it was shown that, as the speed increased so did the muscle activity in players’ lower limbs. There were no significant changes in muscle activity preversus post-protocol. There was however, a general trend of decreasing muscle activity post protocol at higher testing speeds. There were significant (p<0.05) decreases in peak power following the simulated eight over bowling spell. ‘Local’ RPE displayed a significant (P<0.05) increase with each additional over and were observed to reach the ‘heavy’ category. The players’ highest discomfort area was in the lower back, with 13 players perceiving discomfort in this region following the eight over spell. The shoulder and chest were another two areas player’s indicated discomfort with eight players selecting the dominant shoulder. Seven players complained of the dominant side pectoral muscle, leading foot and dominant latissimus dorsi muscle being uncomfortable. Interestingly, the dominant pectoral showed the highest body discomfort ratings amongst players. There were no significant changes in accuracy between overs although there were large interindividual differences in accuracy points between players. The decrease in ball release speed observed during over seven was shown to be significantly (p<0.05) lower than overs one to four. Conclusion: The power output and perceived strain results of the players, appears to indicate the presence of fatigue in players. However, the results are not conclusive, as the fatigue was not shown in muscle recruitment patterns, as well as the body discomfort ratings. There was a non-significant trend observed in the lower limb muscle activation decreasing at higher speeds. Players were able to maintain accuracy. However, the significantly lower ball release speed observed during over seven showed players performance decreasing.
- Full Text:
- Date Issued: 2013
Sustainable harvesting of wild populations of Cyclopia intermedia in Kouga, Eastern Cape, South Africa
- Authors: Barnardo, Nadia
- Date: 2013
- Subjects: Wild plants, Edible -- South Africa -- Eastern Cape , Rooibos tea industry -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10639 , http://hdl.handle.net/10948/d1020195
- Description: Cyclopia intermedia E. Mey is harvested from the wild to produce honeybush tea. The presence of a lignotuber and its slow growth seems to hinder the cultivation of this species and wild harvesting is likely to continue. Species distribution modelling indicated that C. intermedia has a climate envelope that spans from the Eastern Cape to the Western Cape along the Cape Fold mountains. This regional distribution is threatened under future climate change scenarios with a range loss and shift identified for climate conditions predicted for 2050. More specifically, its niche was identified as south-facing slopes with sandy to loamy sand soils and water holding capacity that is higher than the surrounding areas. Some keystone Fynbos species were found to share this niche: Leucadendron salignum, Leucospermum cuneiforme, Protea neriifolia, Protea repens, Elegia filacea and Rhodocoma fruticosa. Cyclopia intermedia grows throughout the summer with flowering buds developing in autumn. These enlarge during winter with the flowers opening in early spring. Pods develop in November and seed set occurs during December. Harvesting plants increases their fecundity with cut plants producing more than twice the number of pods and seeds compared to their non-harvested counterparts. This is because resprouting plants produce more flowering stems than uncut plants. Controlled harvesting or localised short-cycle burning could be considered as a management option to improve fecundity of declining Cyclopia intermedia populations in protected areas. C. intermedia harvesting could occur every third year and stem colour was identified as a measure of harvest maturity. Where at least 30 percent of the population has orange (RHS colour 163A) stems the population is suitable for harvesting.
- Full Text:
- Date Issued: 2013
- Authors: Barnardo, Nadia
- Date: 2013
- Subjects: Wild plants, Edible -- South Africa -- Eastern Cape , Rooibos tea industry -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10639 , http://hdl.handle.net/10948/d1020195
- Description: Cyclopia intermedia E. Mey is harvested from the wild to produce honeybush tea. The presence of a lignotuber and its slow growth seems to hinder the cultivation of this species and wild harvesting is likely to continue. Species distribution modelling indicated that C. intermedia has a climate envelope that spans from the Eastern Cape to the Western Cape along the Cape Fold mountains. This regional distribution is threatened under future climate change scenarios with a range loss and shift identified for climate conditions predicted for 2050. More specifically, its niche was identified as south-facing slopes with sandy to loamy sand soils and water holding capacity that is higher than the surrounding areas. Some keystone Fynbos species were found to share this niche: Leucadendron salignum, Leucospermum cuneiforme, Protea neriifolia, Protea repens, Elegia filacea and Rhodocoma fruticosa. Cyclopia intermedia grows throughout the summer with flowering buds developing in autumn. These enlarge during winter with the flowers opening in early spring. Pods develop in November and seed set occurs during December. Harvesting plants increases their fecundity with cut plants producing more than twice the number of pods and seeds compared to their non-harvested counterparts. This is because resprouting plants produce more flowering stems than uncut plants. Controlled harvesting or localised short-cycle burning could be considered as a management option to improve fecundity of declining Cyclopia intermedia populations in protected areas. C. intermedia harvesting could occur every third year and stem colour was identified as a measure of harvest maturity. Where at least 30 percent of the population has orange (RHS colour 163A) stems the population is suitable for harvesting.
- Full Text:
- Date Issued: 2013
The influence of soil properties on the growth and distribution of Portulacaria Afra in subtropical thicket, South Africa
- Authors: Becker, Carina Helene
- Date: 2013
- Subjects: Portulacaria afra -- South Africa , Plant conservation -- South Africa , Shrubs -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10753 , http://hdl.handle.net/10948/d1013046
- Description: Subtropical Thicket is the dominant vegetation biome in the Eastern Cape, and extends through to parts of the Western Cape. It is dominated by Portulacaria afra (spekboom), a woody succulent plant recognised for its importance as an ecosystem engineer and its carbon sequestration potential. Due to excessive grazing from domestic stock, spekboom has been completely removed from some areas. The Subtropical Thicket Restoration Programme (STRP) initiated a large scale restoration programme of planting spekboom cuttings in these degraded areas. Their efforts have been met with varying levels of success and improvement of the programme relies on continuous monitoring and scientific evaluation. I investigated the influence of selected soil properties on spekboom growth, mortality and landscape distribution, at both restoration sites and natural intact areas, and through experiments. Site or location was the most important factor influencing spekboom success at restoration plots, whereby sites in the eastern end of spekboom distribution perform better. Moving westwards slope orientation emerged as an important factor, whereby north facing slopes are preferred by spekboom. Although high levels of soil salinity (NaCl) restricted spekboom growth and affected its health, it could tolerate the levels it was exposed too. Soil pH, above 7, and phosphorous concentration, above 70 mg.kg-1, were the only limiting factors to spekboom survival found in the restoration sites. This preference for acidic soils was mirrored in intact Thicket. However in general, soil is not a major factor influencing spekboom growth and distribution, and spekboom is tolerant of a wide range of soil conditions. Spekbooms constraint is most likely a function of climate, which varies greatly across the biome. This study answered some vital questions regarding the possible influence of soil in spekboom growth and distribution. It disapproved the theory that a catena effect may be responsible for the lack of spekboom growing in bottomland areas. The study also indicates and supports the versatility of spekboom as a plant for restoring degraded lands across a range of different geologies and soil types. To maximise spekboom survival rates, restoration efforts should be focussed towards the eastern end of its distribution and to avoid planting in soils with pH levels higher than 7.
- Full Text:
- Date Issued: 2013
- Authors: Becker, Carina Helene
- Date: 2013
- Subjects: Portulacaria afra -- South Africa , Plant conservation -- South Africa , Shrubs -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10753 , http://hdl.handle.net/10948/d1013046
- Description: Subtropical Thicket is the dominant vegetation biome in the Eastern Cape, and extends through to parts of the Western Cape. It is dominated by Portulacaria afra (spekboom), a woody succulent plant recognised for its importance as an ecosystem engineer and its carbon sequestration potential. Due to excessive grazing from domestic stock, spekboom has been completely removed from some areas. The Subtropical Thicket Restoration Programme (STRP) initiated a large scale restoration programme of planting spekboom cuttings in these degraded areas. Their efforts have been met with varying levels of success and improvement of the programme relies on continuous monitoring and scientific evaluation. I investigated the influence of selected soil properties on spekboom growth, mortality and landscape distribution, at both restoration sites and natural intact areas, and through experiments. Site or location was the most important factor influencing spekboom success at restoration plots, whereby sites in the eastern end of spekboom distribution perform better. Moving westwards slope orientation emerged as an important factor, whereby north facing slopes are preferred by spekboom. Although high levels of soil salinity (NaCl) restricted spekboom growth and affected its health, it could tolerate the levels it was exposed too. Soil pH, above 7, and phosphorous concentration, above 70 mg.kg-1, were the only limiting factors to spekboom survival found in the restoration sites. This preference for acidic soils was mirrored in intact Thicket. However in general, soil is not a major factor influencing spekboom growth and distribution, and spekboom is tolerant of a wide range of soil conditions. Spekbooms constraint is most likely a function of climate, which varies greatly across the biome. This study answered some vital questions regarding the possible influence of soil in spekboom growth and distribution. It disapproved the theory that a catena effect may be responsible for the lack of spekboom growing in bottomland areas. The study also indicates and supports the versatility of spekboom as a plant for restoring degraded lands across a range of different geologies and soil types. To maximise spekboom survival rates, restoration efforts should be focussed towards the eastern end of its distribution and to avoid planting in soils with pH levels higher than 7.
- Full Text:
- Date Issued: 2013
Search engine poisoning and its prevalence in modern search engines
- Authors: Blaauw, Pieter
- Date: 2013
- Subjects: Web search engines Internet searching World Wide Web Malware (Computer software) Computer viruses Rootkits (Computer software) Spyware (Computer software)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4572 , http://hdl.handle.net/10962/d1002037
- Description: The prevalence of Search Engine Poisoning in trending topics and popular search terms on the web within search engines is investigated. Search Engine Poisoning is the act of manipulating search engines in order to display search results from websites infected with malware. Research done between February and August 2012, using both manual and automated techniques, shows us how easily the criminal element manages to insert malicious content into web pages related to popular search terms within search engines. In order to provide the reader with a clear overview and understanding of the motives and the methods of the operators of Search Engine Poisoning campaigns, an in-depth review of automated and semi-automated web exploit kits is done, as well as looking into the motives for running these campaigns. Three high profile case studies are examined, and the various Search Engine Poisoning campaigns associated with these case studies are discussed in detail to the reader. From February to August 2012, data was collected from the top trending topics on Google’s search engine along with the top listed sites related to these topics, and then passed through various automated tools to discover if these results have been infiltrated by the operators of Search Engine Poisoning campaings, and the results of these automated scans are then discussed in detail. During the research period, manual searching for Search Engine Poisoning campaigns was also done, using high profile news events and popular search terms. These results are analysed in detail to determine the methods of attack, the purpose of the attack and the parties behind it
- Full Text:
- Date Issued: 2013
- Authors: Blaauw, Pieter
- Date: 2013
- Subjects: Web search engines Internet searching World Wide Web Malware (Computer software) Computer viruses Rootkits (Computer software) Spyware (Computer software)
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4572 , http://hdl.handle.net/10962/d1002037
- Description: The prevalence of Search Engine Poisoning in trending topics and popular search terms on the web within search engines is investigated. Search Engine Poisoning is the act of manipulating search engines in order to display search results from websites infected with malware. Research done between February and August 2012, using both manual and automated techniques, shows us how easily the criminal element manages to insert malicious content into web pages related to popular search terms within search engines. In order to provide the reader with a clear overview and understanding of the motives and the methods of the operators of Search Engine Poisoning campaigns, an in-depth review of automated and semi-automated web exploit kits is done, as well as looking into the motives for running these campaigns. Three high profile case studies are examined, and the various Search Engine Poisoning campaigns associated with these case studies are discussed in detail to the reader. From February to August 2012, data was collected from the top trending topics on Google’s search engine along with the top listed sites related to these topics, and then passed through various automated tools to discover if these results have been infiltrated by the operators of Search Engine Poisoning campaings, and the results of these automated scans are then discussed in detail. During the research period, manual searching for Search Engine Poisoning campaigns was also done, using high profile news events and popular search terms. These results are analysed in detail to determine the methods of attack, the purpose of the attack and the parties behind it
- Full Text:
- Date Issued: 2013
Profiling for volatile compounds in the Kgalagadi desert truffle
- Authors: Bogopa, Juda
- Date: 2013
- Subjects: Desert plants -- Botswana -- Kgalagadi District Supercritical fluid extraction Gas chromatography Mass spectrometry Tuberaceae -- Analysis -- Botswana -- Kgalagadi District Truffles -- Analysis -- Botswana -- Kgalagadi District
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4280 , http://hdl.handle.net/10962/d1002006
- Description: The thesis focuses on ‘green’ sample preparation techniques that utilise minimal or no organic solvents thereby producing negligible volumes of organic waste, to ensure safety both to user and the environment. Volatile compounds were extracted and pre-concentrated from Kgalagadi desert truffles (kalaharituber) by headspace solid phase microextraction (HSSPME) and supercritical fluid extraction (SFE). PHWE was employed for the extraction of amino acids and fatty acids. Subsequent analysis of volatile compounds was carried out by gas chromatography coupled with mass spectrometry. Four types of HS-SPME fibers (PDMS 100 μm, PDMS 7 μm, Polyacrylate 85 μm, CAR/DVB/PDMS 50/30 μm) were evaluated. A total of 24 volatile compounds with a molecular weight range from 110 to 354, the most prominent peak being 2-t-Butyl-2,3-dimethyl-3-buten-1-ol (C₁₀H₂₀O, MW 156) were detected after sampling with a PDMS 100 μm fiber. Less volatile compounds were detected after SFE with CO2. A total of 16 amino acids were identified while 17 fatty acids (MW from 132.12 to 367.49) were also identified. The characteristic profile of the Kgalagadi desert truffle was found to contain mainly fatty acid methyl esters and unsaturated aliphatic hydrocarbons. The most prominent compound peaks identified were; 2-t-butyl-2,3-dimethyl- 3-buten-1-ol, disulfide, ethyl benzoic acid 2-4-dhydroxy-3,6-dimethyl-methyl ester, 8,11- octadecanoic acid methyl ester, benzoic acid, 2,4-dihydroxy-3,6-dimethyl-methyl ester, isoquinoline, 1 butyl-3,4-dihydro and 3-heptanone, 6 methyl. Optimization results indicated that fresh slices from the heart of truffles were the best to use for HS-SPME-GCMS volatile compound analysis as they showed a higher sensitivity
- Full Text:
- Date Issued: 2013
- Authors: Bogopa, Juda
- Date: 2013
- Subjects: Desert plants -- Botswana -- Kgalagadi District Supercritical fluid extraction Gas chromatography Mass spectrometry Tuberaceae -- Analysis -- Botswana -- Kgalagadi District Truffles -- Analysis -- Botswana -- Kgalagadi District
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4280 , http://hdl.handle.net/10962/d1002006
- Description: The thesis focuses on ‘green’ sample preparation techniques that utilise minimal or no organic solvents thereby producing negligible volumes of organic waste, to ensure safety both to user and the environment. Volatile compounds were extracted and pre-concentrated from Kgalagadi desert truffles (kalaharituber) by headspace solid phase microextraction (HSSPME) and supercritical fluid extraction (SFE). PHWE was employed for the extraction of amino acids and fatty acids. Subsequent analysis of volatile compounds was carried out by gas chromatography coupled with mass spectrometry. Four types of HS-SPME fibers (PDMS 100 μm, PDMS 7 μm, Polyacrylate 85 μm, CAR/DVB/PDMS 50/30 μm) were evaluated. A total of 24 volatile compounds with a molecular weight range from 110 to 354, the most prominent peak being 2-t-Butyl-2,3-dimethyl-3-buten-1-ol (C₁₀H₂₀O, MW 156) were detected after sampling with a PDMS 100 μm fiber. Less volatile compounds were detected after SFE with CO2. A total of 16 amino acids were identified while 17 fatty acids (MW from 132.12 to 367.49) were also identified. The characteristic profile of the Kgalagadi desert truffle was found to contain mainly fatty acid methyl esters and unsaturated aliphatic hydrocarbons. The most prominent compound peaks identified were; 2-t-butyl-2,3-dimethyl- 3-buten-1-ol, disulfide, ethyl benzoic acid 2-4-dhydroxy-3,6-dimethyl-methyl ester, 8,11- octadecanoic acid methyl ester, benzoic acid, 2,4-dihydroxy-3,6-dimethyl-methyl ester, isoquinoline, 1 butyl-3,4-dihydro and 3-heptanone, 6 methyl. Optimization results indicated that fresh slices from the heart of truffles were the best to use for HS-SPME-GCMS volatile compound analysis as they showed a higher sensitivity
- Full Text:
- Date Issued: 2013
Polyunsaturated fatty acid metabolism and effects on colon cancer cell biology in vitro.
- Authors: Bulcao, Candice
- Date: 2013
- Subjects: Unsaturated fatty acids , Unsaturated fatty acids in human nutrition , Colon (Anatomy)-- Cancer , Cancer -- Nutritional aspects
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4137 , http://hdl.handle.net/10962/d1016128
- Description: Colon cancer is a leading cause of cancer related deaths worldwide. Lifestyle factors such as diet and exercise have been implicated as important agents in colon cancer development and progression. Epidemiological, in vivo and in vitro studies have found that n-3 polyunsaturated fatty acids (PUFAs) reduce colon carcinoma. The role of n-6 PUFAs remains a controversial topic, with studies indicating both promoting and preventing capabilities published. In order to better understand the effects of PUFAs on colon carcinoma, it is important to have an understanding of how they will be broken down in the body. During this study, in silico metabolism of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and arachidonic acid (AA) predicted the formation of hydroxy-, di-hydroxy- and epoxy-FAs. A gas chromatography-mass spectrometry method was developed and validated for the detection of these PUFAs and their cytochrome P450 (CYP) metabolites. A human liver microsomal system for the in vitro metabolism of EPA, DHA and AA was optimised in terms of microsomal and PUFA concentration. The system resulted in the metabolism of the positive control, lauric acid, to 12-hydroxy-lauric acid but was unable to metabolise the PUFAs of interest. EPA, DHA and AA reduced cell viability in the colon carcinoma cell lines SW480 and SW620 in the micromolar concentration range (25 – 200 μM). The CYP epoxidation metabolite of EPA, 17, 18-epoxyeicosatetraenoic acid (17, 18-EpETE) resulted in a significant reduction in SW480 cell viability relative to the parent compound at lower concentrations (25 and 50 μM). Annexin V apoptosis analysis revealed that EPA and 17, 18- EpETE did not result in apoptosis in SW480 cells at a concentration of 25 μM and over an incubation period of 24 hours. A significant reduction in reactive oxygen species production was seen in SW480 cells after incubation with 25 μM 17, 18-EpETE for 24 hours. EPA and 17, 18-EpETE were implicated in the reduction of colon cancer metastasis since they were able to reduce SW480 migration and anchorage independent cell growth. These results indicate that the dietary intake of EPA, DHA and AA may be beneficial to one’s health due to the negative effects that these PUFAs had on colon carcinoma. Future studies are needed to confirm these benefits and compare the effects of the PUFAs to their CYP-metabolites.
- Full Text:
- Date Issued: 2013
- Authors: Bulcao, Candice
- Date: 2013
- Subjects: Unsaturated fatty acids , Unsaturated fatty acids in human nutrition , Colon (Anatomy)-- Cancer , Cancer -- Nutritional aspects
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4137 , http://hdl.handle.net/10962/d1016128
- Description: Colon cancer is a leading cause of cancer related deaths worldwide. Lifestyle factors such as diet and exercise have been implicated as important agents in colon cancer development and progression. Epidemiological, in vivo and in vitro studies have found that n-3 polyunsaturated fatty acids (PUFAs) reduce colon carcinoma. The role of n-6 PUFAs remains a controversial topic, with studies indicating both promoting and preventing capabilities published. In order to better understand the effects of PUFAs on colon carcinoma, it is important to have an understanding of how they will be broken down in the body. During this study, in silico metabolism of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and arachidonic acid (AA) predicted the formation of hydroxy-, di-hydroxy- and epoxy-FAs. A gas chromatography-mass spectrometry method was developed and validated for the detection of these PUFAs and their cytochrome P450 (CYP) metabolites. A human liver microsomal system for the in vitro metabolism of EPA, DHA and AA was optimised in terms of microsomal and PUFA concentration. The system resulted in the metabolism of the positive control, lauric acid, to 12-hydroxy-lauric acid but was unable to metabolise the PUFAs of interest. EPA, DHA and AA reduced cell viability in the colon carcinoma cell lines SW480 and SW620 in the micromolar concentration range (25 – 200 μM). The CYP epoxidation metabolite of EPA, 17, 18-epoxyeicosatetraenoic acid (17, 18-EpETE) resulted in a significant reduction in SW480 cell viability relative to the parent compound at lower concentrations (25 and 50 μM). Annexin V apoptosis analysis revealed that EPA and 17, 18- EpETE did not result in apoptosis in SW480 cells at a concentration of 25 μM and over an incubation period of 24 hours. A significant reduction in reactive oxygen species production was seen in SW480 cells after incubation with 25 μM 17, 18-EpETE for 24 hours. EPA and 17, 18-EpETE were implicated in the reduction of colon cancer metastasis since they were able to reduce SW480 migration and anchorage independent cell growth. These results indicate that the dietary intake of EPA, DHA and AA may be beneficial to one’s health due to the negative effects that these PUFAs had on colon carcinoma. Future studies are needed to confirm these benefits and compare the effects of the PUFAs to their CYP-metabolites.
- Full Text:
- Date Issued: 2013
Evaluation of eucalyptus citriodora derived p-menthane-3,8-diol-citronellal acetal as a bio-plasticizer for cosmetic application
- Authors: Burger, Kirstin
- Date: 2013
- Subjects: Plasticizers , Eucalyptus citriodora
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10420 , http://hdl.handle.net/10948/d1014080
- Description: Plasticizers are generally added to cosmetic and personal care products to improve the filmforming abilities of the product and increase flexibility of the film formed on the skin or hair surface. For example, plasticizers are present in perfumes to prolong the release of the specific scent, which is the ultimate goal in a good quality perfume. Plasticizers in nail varnishes prevent chipping, improve the aesthetics by adhering to the keratin in the nail which means the coating stays on for much longer, which is the ultimate goal in nail products. Plasticizers improve the gloss, resist chipping and allow quick drying time. Therefore it can be seen that plasticizers play a vital role in personal care products like perfumes and nail varnishes. Certain plasticizers e.g. phthalates, can cause problems associated with human health and can harm the environment. They are easily available and large volumes can be obtained at a low cost. These phthalates, for example, di-butyl phthalate (DBP) have been identified as carcinogenic. Nowadays the occurrence of cancer is rapidly increasing. The plasticizers present in a large number of consumer and personal care products, can possibly be linked to the ever increasing reports of cancer. Therefore a substitute to the traditional phthalate plasticizers must be investigated. The aim of this research is to produce a plasticizer derived from naturally occurring Eucalyptus oil, which can be used to replace the existing plasticizers in cosmetic formulations. Para-menthane-3,8-diol (PMD), occurring naturally in the oil from the tree, Eucalyptus citriodora, forms an acetal with citronellal (PMD, acetal, citronellal all occur naturally in the oil). It has been previously shown that PMD-citronellal acetal will exhibit plasticizing properties similar to conventional plasticizers. The objective was to enhance the formation of the acetal in the Eucalyptus oil by reacting it with excess PMD. An effective synthesis method for the PMD-citronellal acetal enriched oil (~73.8 percent) was determined from optimization experiments. The physical characterisation of the PMD-citronellal acetal enriched oil was done and compared with that of DBP. The acetal-enriched oil had a lower density, slightly higher solubility in water (at 25°C), lower refractive index (Brix percent) and a higher boiling point (350°C) than DBP. The physical characteristics of the Eucalyptus oil source and the acetal-enriched Eucalyptus oil were very similar. This can be expected as the Eucalyptus oil consists of ~84.3 percent Citronellal, ~ 1.3 percent PMD and 2.7 percent PMD-citronellal acetal. In this study the effectiveness of the acetal-enriched Eucalyptus oil (referred to from now on as the bio-plasticizer) was compared to a conventional plasticizer such as di-butyl phthalate (DBP), commonly used in cosmetic products. Two cosmetic formulations were produced: a nail varnish and a perfume formulation. Various tests were performed on these formulations to investigate the plasticizing properties of the bio-plasticizer. The objectives were to determine if the natural plasticizer is as effective as the potentially carcinogenic phthalate plasticizers and can be used as a substitute for the phthalates in personal care products. The results indicate that the bio-plasticizer does behave similarly to di-butyl phthalate, however, the effectiveness of the bio-plasticizer is lower than that of di-butyl phthalate. As the viscosity of the synthesized oil was high, this affected the overall consistency of the products. A more viscous nail varnish and perfume was produced in comparison to the DBP counterpart. The stability of the bio-plasticizer in the cosmetic formulations of nail varnish and perfume was also investigated. The cosmetic products were incubated at 0°C, 25°C and 40°C over a period of two months. Any changes in colour, odour, pH, refractive index, separation and plasticizer peak change in the gas chromatogram trace were recorded. It was determined that the PMD-citronellal acetal-enriched oil was relatively unstable under elevated temperatures and light intensity. Storage under higher temperatures (40°C) tends to increase the acidity. Therefore the bio-plasticizer must be placed in a closed, covered bottle and stored in an environment away from light and elevated temperatures. According to the gas chromatogram peaks, it was clear that both the bio-plasticizer and the DBP were more unstable in the perfume formulation than in the nail polish and were especially sensitive to light when in the perfume. This could possibly be due to the interaction with the fragrance molecule, p-anisaldehyde.
- Full Text:
- Date Issued: 2013
- Authors: Burger, Kirstin
- Date: 2013
- Subjects: Plasticizers , Eucalyptus citriodora
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10420 , http://hdl.handle.net/10948/d1014080
- Description: Plasticizers are generally added to cosmetic and personal care products to improve the filmforming abilities of the product and increase flexibility of the film formed on the skin or hair surface. For example, plasticizers are present in perfumes to prolong the release of the specific scent, which is the ultimate goal in a good quality perfume. Plasticizers in nail varnishes prevent chipping, improve the aesthetics by adhering to the keratin in the nail which means the coating stays on for much longer, which is the ultimate goal in nail products. Plasticizers improve the gloss, resist chipping and allow quick drying time. Therefore it can be seen that plasticizers play a vital role in personal care products like perfumes and nail varnishes. Certain plasticizers e.g. phthalates, can cause problems associated with human health and can harm the environment. They are easily available and large volumes can be obtained at a low cost. These phthalates, for example, di-butyl phthalate (DBP) have been identified as carcinogenic. Nowadays the occurrence of cancer is rapidly increasing. The plasticizers present in a large number of consumer and personal care products, can possibly be linked to the ever increasing reports of cancer. Therefore a substitute to the traditional phthalate plasticizers must be investigated. The aim of this research is to produce a plasticizer derived from naturally occurring Eucalyptus oil, which can be used to replace the existing plasticizers in cosmetic formulations. Para-menthane-3,8-diol (PMD), occurring naturally in the oil from the tree, Eucalyptus citriodora, forms an acetal with citronellal (PMD, acetal, citronellal all occur naturally in the oil). It has been previously shown that PMD-citronellal acetal will exhibit plasticizing properties similar to conventional plasticizers. The objective was to enhance the formation of the acetal in the Eucalyptus oil by reacting it with excess PMD. An effective synthesis method for the PMD-citronellal acetal enriched oil (~73.8 percent) was determined from optimization experiments. The physical characterisation of the PMD-citronellal acetal enriched oil was done and compared with that of DBP. The acetal-enriched oil had a lower density, slightly higher solubility in water (at 25°C), lower refractive index (Brix percent) and a higher boiling point (350°C) than DBP. The physical characteristics of the Eucalyptus oil source and the acetal-enriched Eucalyptus oil were very similar. This can be expected as the Eucalyptus oil consists of ~84.3 percent Citronellal, ~ 1.3 percent PMD and 2.7 percent PMD-citronellal acetal. In this study the effectiveness of the acetal-enriched Eucalyptus oil (referred to from now on as the bio-plasticizer) was compared to a conventional plasticizer such as di-butyl phthalate (DBP), commonly used in cosmetic products. Two cosmetic formulations were produced: a nail varnish and a perfume formulation. Various tests were performed on these formulations to investigate the plasticizing properties of the bio-plasticizer. The objectives were to determine if the natural plasticizer is as effective as the potentially carcinogenic phthalate plasticizers and can be used as a substitute for the phthalates in personal care products. The results indicate that the bio-plasticizer does behave similarly to di-butyl phthalate, however, the effectiveness of the bio-plasticizer is lower than that of di-butyl phthalate. As the viscosity of the synthesized oil was high, this affected the overall consistency of the products. A more viscous nail varnish and perfume was produced in comparison to the DBP counterpart. The stability of the bio-plasticizer in the cosmetic formulations of nail varnish and perfume was also investigated. The cosmetic products were incubated at 0°C, 25°C and 40°C over a period of two months. Any changes in colour, odour, pH, refractive index, separation and plasticizer peak change in the gas chromatogram trace were recorded. It was determined that the PMD-citronellal acetal-enriched oil was relatively unstable under elevated temperatures and light intensity. Storage under higher temperatures (40°C) tends to increase the acidity. Therefore the bio-plasticizer must be placed in a closed, covered bottle and stored in an environment away from light and elevated temperatures. According to the gas chromatogram peaks, it was clear that both the bio-plasticizer and the DBP were more unstable in the perfume formulation than in the nail polish and were especially sensitive to light when in the perfume. This could possibly be due to the interaction with the fragrance molecule, p-anisaldehyde.
- Full Text:
- Date Issued: 2013
Log analysis aided by latent semantic mapping
- Authors: Buys, Stephanus
- Date: 2013 , 2013-04-14
- Subjects: Latent semantic indexing , Data mining , Computer networks -- Security measures , Computer hackers , Computer security
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4575 , http://hdl.handle.net/10962/d1002963 , Latent semantic indexing , Data mining , Computer networks -- Security measures , Computer hackers , Computer security
- Description: In an age of zero-day exploits and increased on-line attacks on computing infrastructure, operational security practitioners are becoming increasingly aware of the value of the information captured in log events. Analysis of these events is critical during incident response, forensic investigations related to network breaches, hacking attacks and data leaks. Such analysis has led to the discipline of Security Event Analysis, also known as Log Analysis. There are several challenges when dealing with events, foremost being the increased volumes at which events are often generated and stored. Furthermore, events are often captured as unstructured data, with very little consistency in the formats or contents of the events. In this environment, security analysts and implementers of Log Management (LM) or Security Information and Event Management (SIEM) systems face the daunting task of identifying, classifying and disambiguating massive volumes of events in order for security analysis and automation to proceed. Latent Semantic Mapping (LSM) is a proven paradigm shown to be an effective method of, among other things, enabling word clustering, document clustering, topic clustering and semantic inference. This research is an investigation into the practical application of LSM in the discipline of Security Event Analysis, showing the value of using LSM to assist practitioners in identifying types of events, classifying events as belonging to certain sources or technologies and disambiguating different events from each other. The culmination of this research presents adaptations to traditional natural language processing techniques that resulted in improved efficacy of LSM when dealing with Security Event Analysis. This research provides strong evidence supporting the wider adoption and use of LSM, as well as further investigation into Security Event Analysis assisted by LSM and other natural language or computer-learning processing techniques. , LaTeX with hyperref package , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Date Issued: 2013
- Authors: Buys, Stephanus
- Date: 2013 , 2013-04-14
- Subjects: Latent semantic indexing , Data mining , Computer networks -- Security measures , Computer hackers , Computer security
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4575 , http://hdl.handle.net/10962/d1002963 , Latent semantic indexing , Data mining , Computer networks -- Security measures , Computer hackers , Computer security
- Description: In an age of zero-day exploits and increased on-line attacks on computing infrastructure, operational security practitioners are becoming increasingly aware of the value of the information captured in log events. Analysis of these events is critical during incident response, forensic investigations related to network breaches, hacking attacks and data leaks. Such analysis has led to the discipline of Security Event Analysis, also known as Log Analysis. There are several challenges when dealing with events, foremost being the increased volumes at which events are often generated and stored. Furthermore, events are often captured as unstructured data, with very little consistency in the formats or contents of the events. In this environment, security analysts and implementers of Log Management (LM) or Security Information and Event Management (SIEM) systems face the daunting task of identifying, classifying and disambiguating massive volumes of events in order for security analysis and automation to proceed. Latent Semantic Mapping (LSM) is a proven paradigm shown to be an effective method of, among other things, enabling word clustering, document clustering, topic clustering and semantic inference. This research is an investigation into the practical application of LSM in the discipline of Security Event Analysis, showing the value of using LSM to assist practitioners in identifying types of events, classifying events as belonging to certain sources or technologies and disambiguating different events from each other. The culmination of this research presents adaptations to traditional natural language processing techniques that resulted in improved efficacy of LSM when dealing with Security Event Analysis. This research provides strong evidence supporting the wider adoption and use of LSM, as well as further investigation into Security Event Analysis assisted by LSM and other natural language or computer-learning processing techniques. , LaTeX with hyperref package , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Date Issued: 2013
The factors affecting self-regulation through the analysis of physiological, psychological and behavioural measures during task-switching
- Authors: Chaplin, Caley
- Date: 2013 , 2013-07-16
- Subjects: Human multitasking -- Physiological aspects , Human multitasking -- Psychological aspects , Fatigue , Boredom , Attention , Mental fatigue , Human information processing , Decision making , Labor productivity , Industrial hygiene , Employees -- Workload
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5135 , http://hdl.handle.net/10962/d1006027 , Human multitasking -- Physiological aspects , Human multitasking -- Psychological aspects , Fatigue , Boredom , Attention , Mental fatigue , Human information processing , Decision making , Labor productivity , Industrial hygiene , Employees -- Workload
- Description: Individuals are required to manage multiple tasks which require strategic allocation of time and effort to ensure goals are reached efficiently. By providing the worker with autonomy over their work, performance and worker well-being have improved. This increased control allows individuals to organize work according to the needs of the body, which prevents fatigue leading to improved productivity. When given the option, humans tend to switch between tasks frequently. This behaviour can be used to determine the change in self-regulation strategies. An understanding of human task-switching behaviour is important for the design of job rotation systems. However, there is a lack of evidence explaining the factors motivating the need to switch between tasks. This study aims to use physiological, subjective and behavioural measures to explain the factors influencing selfregulation through the act of task-switching. Three primary hypotheses were developed to explain the factors underlying taskswitching behaviour. It was hypothesized that the degree of boredom experienced, the effort required to perform the task and the resource usage induced by the task are factors responsible in deciding task switching behaviour. Participants (17 males and 17 females) switched freely between five different information-processing tasks for the 45 minutes. Participants were allowed to switch back and forth between tasks and did not have to conduct all five tasks. The following measures were recorded during the experiment: subjective measures of boredom, mental effort, task frustration and perceived performance of the tasks; energy consumption and physiological measures of effort (HR, HRV and body temperature) and behavioural measures, including duration and frequency of task. Perceived boredom was found to differ among the tasks and before and after the experiment. The average boredom rating at each task transition for all tasks exceeded a score of 2.5 out of a possible 4. There were no significant changes in physiological measures between the beginning and end of the task trials. However, changes in physiological measures showed a decrease in effort investment following task transition. Heart rate variability was lower for externally-paced tasks than for self-paced tasks, despite the differences in cognitive demands. The most frequent task-switch combination occurred between tasks of high and low cognitive demand. The least frequent task-switching combination occurred between tasks of similar characteristics, which produced no differences in physiological responses. Task-switching behaviour was influenced by the degree of boredom, and therefore more time was spent on less monotonous tasks. The level of physiological effort required for the task affected task-switching behaviour. Task switches were made before any changes in effort took place in an attempt to maintain task efficiency. It appears plausible that a task switch was made to reduce effort investment and activation levels. The type of information processing resources used by different tasks affected the task-switching combinations. Individuals tended to switch between tasks of differing resources so that those in limited supply were able to replenish. Therefore the findings from this study can potentially be used to improve the design of job rotation systems. Such improvements may enhance productivity and worker well-being by inhibiting the onset of down regulation and fatigue processes. This study showed that autonomy is necessary for individuals to regulate behaviour to suit human needs. , Microsoft� Office Word 2007 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Date Issued: 2013
- Authors: Chaplin, Caley
- Date: 2013 , 2013-07-16
- Subjects: Human multitasking -- Physiological aspects , Human multitasking -- Psychological aspects , Fatigue , Boredom , Attention , Mental fatigue , Human information processing , Decision making , Labor productivity , Industrial hygiene , Employees -- Workload
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5135 , http://hdl.handle.net/10962/d1006027 , Human multitasking -- Physiological aspects , Human multitasking -- Psychological aspects , Fatigue , Boredom , Attention , Mental fatigue , Human information processing , Decision making , Labor productivity , Industrial hygiene , Employees -- Workload
- Description: Individuals are required to manage multiple tasks which require strategic allocation of time and effort to ensure goals are reached efficiently. By providing the worker with autonomy over their work, performance and worker well-being have improved. This increased control allows individuals to organize work according to the needs of the body, which prevents fatigue leading to improved productivity. When given the option, humans tend to switch between tasks frequently. This behaviour can be used to determine the change in self-regulation strategies. An understanding of human task-switching behaviour is important for the design of job rotation systems. However, there is a lack of evidence explaining the factors motivating the need to switch between tasks. This study aims to use physiological, subjective and behavioural measures to explain the factors influencing selfregulation through the act of task-switching. Three primary hypotheses were developed to explain the factors underlying taskswitching behaviour. It was hypothesized that the degree of boredom experienced, the effort required to perform the task and the resource usage induced by the task are factors responsible in deciding task switching behaviour. Participants (17 males and 17 females) switched freely between five different information-processing tasks for the 45 minutes. Participants were allowed to switch back and forth between tasks and did not have to conduct all five tasks. The following measures were recorded during the experiment: subjective measures of boredom, mental effort, task frustration and perceived performance of the tasks; energy consumption and physiological measures of effort (HR, HRV and body temperature) and behavioural measures, including duration and frequency of task. Perceived boredom was found to differ among the tasks and before and after the experiment. The average boredom rating at each task transition for all tasks exceeded a score of 2.5 out of a possible 4. There were no significant changes in physiological measures between the beginning and end of the task trials. However, changes in physiological measures showed a decrease in effort investment following task transition. Heart rate variability was lower for externally-paced tasks than for self-paced tasks, despite the differences in cognitive demands. The most frequent task-switch combination occurred between tasks of high and low cognitive demand. The least frequent task-switching combination occurred between tasks of similar characteristics, which produced no differences in physiological responses. Task-switching behaviour was influenced by the degree of boredom, and therefore more time was spent on less monotonous tasks. The level of physiological effort required for the task affected task-switching behaviour. Task switches were made before any changes in effort took place in an attempt to maintain task efficiency. It appears plausible that a task switch was made to reduce effort investment and activation levels. The type of information processing resources used by different tasks affected the task-switching combinations. Individuals tended to switch between tasks of differing resources so that those in limited supply were able to replenish. Therefore the findings from this study can potentially be used to improve the design of job rotation systems. Such improvements may enhance productivity and worker well-being by inhibiting the onset of down regulation and fatigue processes. This study showed that autonomy is necessary for individuals to regulate behaviour to suit human needs. , Microsoft� Office Word 2007 , Adobe Acrobat 9.54 Paper Capture Plug-in
- Full Text:
- Date Issued: 2013
An assessment of urban residents' knowledge and appreciation of the intangible benefits of trees in two medium sized towns in South Africa
- Authors: Chinyimba, Abby
- Date: 2013
- Subjects: Trees in cities -- South Africa--Limpopo , Cities and towns -- South Africa -- Limpopo , Ecology -- South Africa -- Limpopo , Human-plant relationships -- South Africa -- Limpopo , Plants and civilization -- South Africa -- Limpopo , Willingness to pay -- South Africa -- Limpopo , Limpopo (South Africa) -- Environmental conditions , Cultural importance , Cultural and regulating services , Intangible benefits , Urban systems
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4730 , http://hdl.handle.net/10962/d1001957
- Description: In order to meet the different public needs and requirements of contemporary urban society, a better understanding of public perceptions, appreciation and knowledge of the assorted values derived from urban trees is beneficial for improved urban greening. Thus, this study carried out an assessment of urban residents’ knowledge and appreciation of the benefits of trees and the role they play in enhancing the quality of life in urban systems. The study primarily focused on the intangible benefits (also referred to as cultural and regulating services) of urban trees, because most research to date in South Africa on trees and the benefits derived from them is biased towards the provisioning services of trees in rural areas. The significance and contribution of the intangible benefits of trees in urban systems is often underestimated and not seriously taken into account by urban planners, policy makers and other key decision makers. The study was conducted in three different residential settlement types (formal townships, RDP settlements and informal settlements) in two towns in the Limpopo Province in South Africa, namely Bela-Bela and Tzaneen. Findings revealed that the intangible benefits of trees are seen to play a significant role in improving both residents’ quality of life and the environmental conditions in cities, including the preservation of biodiversity. Findings also showed that urban residents had strong spiritual connections with trees and placed considerable cultural and historical importance on specific tree species and individual trees. Urban residents in both towns had moderate knowledge of the intangible benefits of trees based on the knowledge score that was created for the purposes of this study. Fifty seven percent of the residents had ‘reasonable’ knowledge of some of the intangible benefits of trees, while 43% had low levels of knowledge. That said, the majority of residents demonstrated high appreciation (98%) of the intangible benefits of trees (especially social and cultural values), with few residents not recognising these, suggesting that knowledge does not necessarily lead to appreciation. Age and level of education were found to influence knowledge of the intangible benefits of trees. A large proportion (86%) of residents who possessed tertiary level qualifications had more knowledge of the intangible benefits of trees as compared to residents with no formal education. Findings also revealed that younger respondents (60%) had more knowledge of the intangible benefits of trees. Factors that influenced appreciation of the intangible benefits of trees included links to crime, peoples’ value systems (their pro-environmental and altruistic values) and residential settlement type. Sixty five percent of residents in both towns felt that urban street trees and trees in public spaces attract criminals and promote crime, while 67% of RDP and 96% of informal township residents did not use or appreciate the social and recreational benefits of trees mainly because of the absence or long distances to public parks. A Willingness To Pay (WTP) survey revealed that a high proportion of residents in Bela-Bela (86%) were WTP a small annual fee in order to sustain greening initiatives in their communities compared to the residents in Tzaneen (53%). In terms of the planting and retention of trees, it was found that 300 households in both study towns had planted and retained a total of 1 615 trees in spite of the various factors that negatively influenced planting and retention of trees such as residency time and tenure security. The majority (66%) of informal township residents said tenure security was an important factor to consider when making tree planting decisions, while this was not an issue for formal township and RDP residents. Policy implications and recommendations are presented to help municipalities and urban planners improve and develop effective policies and programmes that will enable implementation of sustainable urban greening programmes
- Full Text:
- Date Issued: 2013
- Authors: Chinyimba, Abby
- Date: 2013
- Subjects: Trees in cities -- South Africa--Limpopo , Cities and towns -- South Africa -- Limpopo , Ecology -- South Africa -- Limpopo , Human-plant relationships -- South Africa -- Limpopo , Plants and civilization -- South Africa -- Limpopo , Willingness to pay -- South Africa -- Limpopo , Limpopo (South Africa) -- Environmental conditions , Cultural importance , Cultural and regulating services , Intangible benefits , Urban systems
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4730 , http://hdl.handle.net/10962/d1001957
- Description: In order to meet the different public needs and requirements of contemporary urban society, a better understanding of public perceptions, appreciation and knowledge of the assorted values derived from urban trees is beneficial for improved urban greening. Thus, this study carried out an assessment of urban residents’ knowledge and appreciation of the benefits of trees and the role they play in enhancing the quality of life in urban systems. The study primarily focused on the intangible benefits (also referred to as cultural and regulating services) of urban trees, because most research to date in South Africa on trees and the benefits derived from them is biased towards the provisioning services of trees in rural areas. The significance and contribution of the intangible benefits of trees in urban systems is often underestimated and not seriously taken into account by urban planners, policy makers and other key decision makers. The study was conducted in three different residential settlement types (formal townships, RDP settlements and informal settlements) in two towns in the Limpopo Province in South Africa, namely Bela-Bela and Tzaneen. Findings revealed that the intangible benefits of trees are seen to play a significant role in improving both residents’ quality of life and the environmental conditions in cities, including the preservation of biodiversity. Findings also showed that urban residents had strong spiritual connections with trees and placed considerable cultural and historical importance on specific tree species and individual trees. Urban residents in both towns had moderate knowledge of the intangible benefits of trees based on the knowledge score that was created for the purposes of this study. Fifty seven percent of the residents had ‘reasonable’ knowledge of some of the intangible benefits of trees, while 43% had low levels of knowledge. That said, the majority of residents demonstrated high appreciation (98%) of the intangible benefits of trees (especially social and cultural values), with few residents not recognising these, suggesting that knowledge does not necessarily lead to appreciation. Age and level of education were found to influence knowledge of the intangible benefits of trees. A large proportion (86%) of residents who possessed tertiary level qualifications had more knowledge of the intangible benefits of trees as compared to residents with no formal education. Findings also revealed that younger respondents (60%) had more knowledge of the intangible benefits of trees. Factors that influenced appreciation of the intangible benefits of trees included links to crime, peoples’ value systems (their pro-environmental and altruistic values) and residential settlement type. Sixty five percent of residents in both towns felt that urban street trees and trees in public spaces attract criminals and promote crime, while 67% of RDP and 96% of informal township residents did not use or appreciate the social and recreational benefits of trees mainly because of the absence or long distances to public parks. A Willingness To Pay (WTP) survey revealed that a high proportion of residents in Bela-Bela (86%) were WTP a small annual fee in order to sustain greening initiatives in their communities compared to the residents in Tzaneen (53%). In terms of the planting and retention of trees, it was found that 300 households in both study towns had planted and retained a total of 1 615 trees in spite of the various factors that negatively influenced planting and retention of trees such as residency time and tenure security. The majority (66%) of informal township residents said tenure security was an important factor to consider when making tree planting decisions, while this was not an issue for formal township and RDP residents. Policy implications and recommendations are presented to help municipalities and urban planners improve and develop effective policies and programmes that will enable implementation of sustainable urban greening programmes
- Full Text:
- Date Issued: 2013
Governance and management of urban trees and green spaces in South Africa: ensuring benefits to local people and the environment
- Authors: Chishaleshale, Mwale
- Date: 2013
- Subjects: Trees in cities -- South Africa , Urban forestry -- South Africa , Community forestry -- South Africa , City planning -- South Africa , Human-plant relationships -- South Africa , Urban ecology (Biology -- South Africa , Urban ecology (Sociology) -- South Africa , Natural resources -- South Africa , Trees in cities -- Law and legislation -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4739 , http://hdl.handle.net/10962/d1006035 , Trees in cities -- South Africa , Urban forestry -- South Africa , Community forestry -- South Africa , City planning -- South Africa , Human-plant relationships -- South Africa , Urban ecology (Biology -- South Africa , Urban ecology (Sociology) -- South Africa , Natural resources -- South Africa , Trees in cities -- Law and legislation -- South Africa
- Description: In the face of rapid urbanization and global climate change, urban trees and green spaces (UTGS) can contribute to the welfare of people and the urban environment. Urban trees and green spaces can assist to address urbanization challenges related to environmental degradation. While functions of UTGS have been well documented in the developed world, they have not yet received full attention in much of sub-Saharan Africa. Consequently, UTGS are under threat from urban development and fragmentation. Notably, the problems associated with UTGS also fall into the governance realm and indications are that poor governance and management of UTGS can negatively influence the potential benefits of UTGS to local people and the environment. This formed a basis for this research. The main objective of the study was to determine the current governance and management approaches to UTGS in South Africa. Through document search and review, the study determined the governance institutions influencing UTGS at national level and at provincial level (in the Limpopo and Eastern Cape Provinces). Face-to-face and online survey methods were used to determine the extent to which 28 local municipalities had adopted planned, systematic and integrated management of UTGS. The snowball approach was used to determine the key actors involved in UTGS activities and interviews were conducted to establish the roles and capabilities of these actors. A total of 540 household interviews were conducted to determine the institutional factors influencing local peoples’ ability to access, plant and use UTGS. The findings of the study showed that UTGS have not been adequately covered in existing governance institutions and practice at national and provincial levels. Local government municipalities were not managing their UTGS in a planned or systematic manner due to constraining factors such as insufficient funds, insufficient personnel, lack of equipment and lack of political support. Only 7.1 % of the surveyed municipalities had an urban tree management plan and an estimate of the urban tree stock; 32.1 % had tree policies; 28.6 % had tree bylaws; 21.4 % had tree planting schedules; 10.7 % had tree maintenance schedules and only 3.6 % had tree inspection schedules. Key actors involved in UTGS activities differed among levels of government. The actors included national and provincial government departments, local government municipalities, Non-Governmental Organizations, private sector companies and local volunteers. Most of the actors, however, either planted trees or provided tree seedlings to municipalities and the local people. Tenure security was a key institutional factor affecting peoples’ ability to plant, use or even remove trees from their residential plots. The same applied to trees in the streets and public parks. Whereas most respondents did not require permission to plant (79.8 %) or remove (75 %) trees on their residential plots, a majority of them required permission to plant and remove trees from streets (over 70 %) and public parks (over 80 %). However, with regard to planting and removing urban trees in public open spaces, 54% of the respondents indicated that permission was not required suggesting a lack of clarity among local residents on the issue. Overall, the findings of this study indicate that there is no political recognition and support for UTGS at almost all levels of government. This has resulted in the lack of incorporation of UTGS in urban planning and development and has caused UTGS to receive limited funding to permit planned and systematic management. Given the current rates of urbanization and urban development, the lowly prioritised UTGS are vulnerable to exploitation. To conserve UTGS and promote their potential contribution to local people and the environment, UTGS must be recognized and placed on political and development agendas. There is a need to develop national guidelines for UTGS management, assess the extent of the urban forest resource in local municipalities, clearly define the roles and capabilities of different actors, integrate UTGS in the urban planning and development system, and most of all seek to involve the local people in overall management and governance of UTGS.
- Full Text:
- Date Issued: 2013
- Authors: Chishaleshale, Mwale
- Date: 2013
- Subjects: Trees in cities -- South Africa , Urban forestry -- South Africa , Community forestry -- South Africa , City planning -- South Africa , Human-plant relationships -- South Africa , Urban ecology (Biology -- South Africa , Urban ecology (Sociology) -- South Africa , Natural resources -- South Africa , Trees in cities -- Law and legislation -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4739 , http://hdl.handle.net/10962/d1006035 , Trees in cities -- South Africa , Urban forestry -- South Africa , Community forestry -- South Africa , City planning -- South Africa , Human-plant relationships -- South Africa , Urban ecology (Biology -- South Africa , Urban ecology (Sociology) -- South Africa , Natural resources -- South Africa , Trees in cities -- Law and legislation -- South Africa
- Description: In the face of rapid urbanization and global climate change, urban trees and green spaces (UTGS) can contribute to the welfare of people and the urban environment. Urban trees and green spaces can assist to address urbanization challenges related to environmental degradation. While functions of UTGS have been well documented in the developed world, they have not yet received full attention in much of sub-Saharan Africa. Consequently, UTGS are under threat from urban development and fragmentation. Notably, the problems associated with UTGS also fall into the governance realm and indications are that poor governance and management of UTGS can negatively influence the potential benefits of UTGS to local people and the environment. This formed a basis for this research. The main objective of the study was to determine the current governance and management approaches to UTGS in South Africa. Through document search and review, the study determined the governance institutions influencing UTGS at national level and at provincial level (in the Limpopo and Eastern Cape Provinces). Face-to-face and online survey methods were used to determine the extent to which 28 local municipalities had adopted planned, systematic and integrated management of UTGS. The snowball approach was used to determine the key actors involved in UTGS activities and interviews were conducted to establish the roles and capabilities of these actors. A total of 540 household interviews were conducted to determine the institutional factors influencing local peoples’ ability to access, plant and use UTGS. The findings of the study showed that UTGS have not been adequately covered in existing governance institutions and practice at national and provincial levels. Local government municipalities were not managing their UTGS in a planned or systematic manner due to constraining factors such as insufficient funds, insufficient personnel, lack of equipment and lack of political support. Only 7.1 % of the surveyed municipalities had an urban tree management plan and an estimate of the urban tree stock; 32.1 % had tree policies; 28.6 % had tree bylaws; 21.4 % had tree planting schedules; 10.7 % had tree maintenance schedules and only 3.6 % had tree inspection schedules. Key actors involved in UTGS activities differed among levels of government. The actors included national and provincial government departments, local government municipalities, Non-Governmental Organizations, private sector companies and local volunteers. Most of the actors, however, either planted trees or provided tree seedlings to municipalities and the local people. Tenure security was a key institutional factor affecting peoples’ ability to plant, use or even remove trees from their residential plots. The same applied to trees in the streets and public parks. Whereas most respondents did not require permission to plant (79.8 %) or remove (75 %) trees on their residential plots, a majority of them required permission to plant and remove trees from streets (over 70 %) and public parks (over 80 %). However, with regard to planting and removing urban trees in public open spaces, 54% of the respondents indicated that permission was not required suggesting a lack of clarity among local residents on the issue. Overall, the findings of this study indicate that there is no political recognition and support for UTGS at almost all levels of government. This has resulted in the lack of incorporation of UTGS in urban planning and development and has caused UTGS to receive limited funding to permit planned and systematic management. Given the current rates of urbanization and urban development, the lowly prioritised UTGS are vulnerable to exploitation. To conserve UTGS and promote their potential contribution to local people and the environment, UTGS must be recognized and placed on political and development agendas. There is a need to develop national guidelines for UTGS management, assess the extent of the urban forest resource in local municipalities, clearly define the roles and capabilities of different actors, integrate UTGS in the urban planning and development system, and most of all seek to involve the local people in overall management and governance of UTGS.
- Full Text:
- Date Issued: 2013
Responses to the linked stressors of climate change and HIV/AIDS amongst vulnerable rural households in the Eastern Cape, South Africa
- Authors: Clarke, Caryn Lee
- Date: 2013
- Subjects: Climatic changes -- South Africa -- Eastern Cape Climatic changes -- Health aspects -- South Africa -- Eastern Cape Climatic changes -- Economic aspects -- South Africa -- Eastern Cape HIV infections -- Economic aspects -- South Africa -- Eastern Cape AIDS (Disease) -- Economic aspects -- South Africa -- Eastern Cape HIV infections -- Social aspects -- South Africa -- Eastern Cape AIDS (Disease) -- Social aspects -- South Africa -- Eastern Cape Poverty -- Social aspects -- South Africa -- Eastern Cape Income -- South Africa -- Eastern Cape Human capital -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4732 , http://hdl.handle.net/10962/d1003818
- Description: Climate change and the HIV/AIDS epidemic are two of the most critical long-term global challenges, especially for Africa and even more so Southern Africa. There is great concern that the poor will be unable to adapt to the impacts of climate variability and change while HIV/AIDS will exacerbate the impacts of such stressors and deepen the insecurities of many communities already affected by this disease. Studies that consider the interlinked effects of climate change and HIV/AIDS along with other multiple stressors are increasingly needed. This study, located in two rural communities in the Eastern Cape Province of South Africa, namely Lesseyton and Willowvale, assessed the responses of vulnerable households to the linked shocks and stressors of climate change and HIV/AIDS. This involved assessing, through household surveys, life history interviews and Participatory Learning and Action (PLA), the way in which multiple stressors interacted and affected vulnerable households, the way in which these households responded to and coped with such shocks and stressors, and the barriers which prevented them from coping and adapting effectively. Unemployment emerged as the dominant stress amongst households. The lack of development and having too few opportunities for employment has limited vulnerable households from being able to invest in assets, such as education or farming equipment. This, in combination with the impacts of increased food and water insecurity from recent drought, has created an extremely vulnerable environment for these households. They rely largely on two important safety-nets, namely social capital and the use of natural and cultivated resources; however the latter has been limited due to the impacts of water scarcity and an inability to farm. It was evident that there was little planned long-term adaptation amongst households and from government. Maladaptive short-term coping strategies, such numerous household members depending on one social grant and transactional sex, were too often relied upon, and although they may have helped relieve the stress of shocks momentarily, they did not provide for the long-term well-being of individuals and households. Poor communication and capacity between the different levels of government and between the government (especially at the local level) and the two rural communities has created an environment full of uncertainty and lacking in advocacy. Local government needs increased human, informational, and financial capacity and a clear delegation of responsibilities amongst the different departments in order for the two communities to benefit from the implementation of support strategies. There is also a great need for educational programmes and capacity development within the two rural communities, particularly based on improved coping and longer-term adaptation strategies in response to climate change in order for households to better prepare themselves for the future.
- Full Text:
- Date Issued: 2013
- Authors: Clarke, Caryn Lee
- Date: 2013
- Subjects: Climatic changes -- South Africa -- Eastern Cape Climatic changes -- Health aspects -- South Africa -- Eastern Cape Climatic changes -- Economic aspects -- South Africa -- Eastern Cape HIV infections -- Economic aspects -- South Africa -- Eastern Cape AIDS (Disease) -- Economic aspects -- South Africa -- Eastern Cape HIV infections -- Social aspects -- South Africa -- Eastern Cape AIDS (Disease) -- Social aspects -- South Africa -- Eastern Cape Poverty -- Social aspects -- South Africa -- Eastern Cape Income -- South Africa -- Eastern Cape Human capital -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4732 , http://hdl.handle.net/10962/d1003818
- Description: Climate change and the HIV/AIDS epidemic are two of the most critical long-term global challenges, especially for Africa and even more so Southern Africa. There is great concern that the poor will be unable to adapt to the impacts of climate variability and change while HIV/AIDS will exacerbate the impacts of such stressors and deepen the insecurities of many communities already affected by this disease. Studies that consider the interlinked effects of climate change and HIV/AIDS along with other multiple stressors are increasingly needed. This study, located in two rural communities in the Eastern Cape Province of South Africa, namely Lesseyton and Willowvale, assessed the responses of vulnerable households to the linked shocks and stressors of climate change and HIV/AIDS. This involved assessing, through household surveys, life history interviews and Participatory Learning and Action (PLA), the way in which multiple stressors interacted and affected vulnerable households, the way in which these households responded to and coped with such shocks and stressors, and the barriers which prevented them from coping and adapting effectively. Unemployment emerged as the dominant stress amongst households. The lack of development and having too few opportunities for employment has limited vulnerable households from being able to invest in assets, such as education or farming equipment. This, in combination with the impacts of increased food and water insecurity from recent drought, has created an extremely vulnerable environment for these households. They rely largely on two important safety-nets, namely social capital and the use of natural and cultivated resources; however the latter has been limited due to the impacts of water scarcity and an inability to farm. It was evident that there was little planned long-term adaptation amongst households and from government. Maladaptive short-term coping strategies, such numerous household members depending on one social grant and transactional sex, were too often relied upon, and although they may have helped relieve the stress of shocks momentarily, they did not provide for the long-term well-being of individuals and households. Poor communication and capacity between the different levels of government and between the government (especially at the local level) and the two rural communities has created an environment full of uncertainty and lacking in advocacy. Local government needs increased human, informational, and financial capacity and a clear delegation of responsibilities amongst the different departments in order for the two communities to benefit from the implementation of support strategies. There is also a great need for educational programmes and capacity development within the two rural communities, particularly based on improved coping and longer-term adaptation strategies in response to climate change in order for households to better prepare themselves for the future.
- Full Text:
- Date Issued: 2013
In-silico analysis of Plasmodium falciparum Hop protein and its interactions with Hsp70 and Hsp90
- Authors: Clitheroe, Crystal-Leigh
- Date: 2013
- Subjects: Plasmodium falciparum , Heat shock proteins , Molecular chaperones , Homology (Biology) , Protein-protein interactions , Malaria -- Chemotherapy
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3896 , http://hdl.handle.net/10962/d1003819 , Plasmodium falciparum , Heat shock proteins , Molecular chaperones , Homology (Biology) , Protein-protein interactions , Malaria -- Chemotherapy
- Description: A lessor understood co-chaperone, the Hsp70/Hsp90 organising protein (Hop), has been found to play an important role in modulating the activity and co-interaction of two essential chaperones; Hsp90 and Hsp70. The best understood aspects of Hop so far indicate that residues in the concave surfaces of the three tetratricopeptide repeat (TPR) domains in the protein bind selectively to the C-terminal motifs of Hsp70 and Hsp90. Recent research suggests that P. falciparum Hop (PfHop), PfHsp90 and PfHsp70 do interact and form complex in the P. falciparum trophozooite and are overexpressed in this infective stage. However, there has been almost no computational research on malarial Hop protein in complex with other malarial Hsps.The current work has focussed on several aspects of the in-silico characterisation of PfHop, including an in-depth multiple sequence alignment and phylogenetic analysis of the protein; which showed that Hop is very well conserved across a wide range of available phyla (four Kingdoms, 60 species). Homology modelling was employed to predict several protein structures for these interactions in P. falciparum, as well as predict structures of the relevant TPR domains of Human Hop (HsHop) in complex with its own Hsp90 and Hsp70 C-terminal peptide partners for comparison. Protein complex interaction analyses indicate that concave TPR sites bound to the C-terminal motifs of partner proteins are very similar in both species, due to the excellent conservation of the TPR domain’s “double carboxylate binding clamp”. Motif analysis was combined with phylogenetic trees and structure mapping in novel ways to attain more information on the evolutionary conservation of important structural and functional sites on Hop. Alternative sites of interaction between Hop TPR2 and Hsp90’s M and C domains are distinctly less well conserved between the two species, but still important to complex formation, making this a likely interaction site for selective drug targeting. Binding and interaction energies for all modelled complexes have been calculated; indicating that all HsHop TPR domains have higher affinities for their respective C-terminal partners than do their P. falciparum counterparts. An alternate motif corresponding to the C-terminal motif of PfHsp70-x (exported to the infected erythrocyte cytosol) in complex with both human and malarial TPR1 and TPR2B domains was analysed, and these studies suggest that the human TPR domains have a higher affinity for this motif than do the respective PfHop TPR domains. This may indicate potential for a cross species protein interaction to take place, as PfHop is not transported to the human erythrocyte cytosol.
- Full Text:
- Date Issued: 2013
- Authors: Clitheroe, Crystal-Leigh
- Date: 2013
- Subjects: Plasmodium falciparum , Heat shock proteins , Molecular chaperones , Homology (Biology) , Protein-protein interactions , Malaria -- Chemotherapy
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3896 , http://hdl.handle.net/10962/d1003819 , Plasmodium falciparum , Heat shock proteins , Molecular chaperones , Homology (Biology) , Protein-protein interactions , Malaria -- Chemotherapy
- Description: A lessor understood co-chaperone, the Hsp70/Hsp90 organising protein (Hop), has been found to play an important role in modulating the activity and co-interaction of two essential chaperones; Hsp90 and Hsp70. The best understood aspects of Hop so far indicate that residues in the concave surfaces of the three tetratricopeptide repeat (TPR) domains in the protein bind selectively to the C-terminal motifs of Hsp70 and Hsp90. Recent research suggests that P. falciparum Hop (PfHop), PfHsp90 and PfHsp70 do interact and form complex in the P. falciparum trophozooite and are overexpressed in this infective stage. However, there has been almost no computational research on malarial Hop protein in complex with other malarial Hsps.The current work has focussed on several aspects of the in-silico characterisation of PfHop, including an in-depth multiple sequence alignment and phylogenetic analysis of the protein; which showed that Hop is very well conserved across a wide range of available phyla (four Kingdoms, 60 species). Homology modelling was employed to predict several protein structures for these interactions in P. falciparum, as well as predict structures of the relevant TPR domains of Human Hop (HsHop) in complex with its own Hsp90 and Hsp70 C-terminal peptide partners for comparison. Protein complex interaction analyses indicate that concave TPR sites bound to the C-terminal motifs of partner proteins are very similar in both species, due to the excellent conservation of the TPR domain’s “double carboxylate binding clamp”. Motif analysis was combined with phylogenetic trees and structure mapping in novel ways to attain more information on the evolutionary conservation of important structural and functional sites on Hop. Alternative sites of interaction between Hop TPR2 and Hsp90’s M and C domains are distinctly less well conserved between the two species, but still important to complex formation, making this a likely interaction site for selective drug targeting. Binding and interaction energies for all modelled complexes have been calculated; indicating that all HsHop TPR domains have higher affinities for their respective C-terminal partners than do their P. falciparum counterparts. An alternate motif corresponding to the C-terminal motif of PfHsp70-x (exported to the infected erythrocyte cytosol) in complex with both human and malarial TPR1 and TPR2B domains was analysed, and these studies suggest that the human TPR domains have a higher affinity for this motif than do the respective PfHop TPR domains. This may indicate potential for a cross species protein interaction to take place, as PfHop is not transported to the human erythrocyte cytosol.
- Full Text:
- Date Issued: 2013
A standardised protocol for roadkill detection and the determinants of roadkill in the greater Mapungubwe Transfrontier Conservation Area, Limpopo Province, South Africa
- Authors: Collinson, Wendy Jane
- Date: 2013
- Subjects: Roadkill -- South Africa -- Mapungubwe Site , Traffic safety and wildlife -- South Africa -- Mapungubwe Site
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5606 , http://hdl.handle.net/10962/d1002056 , Roadkill -- South Africa -- Mapungubwe Site , Traffic safety and wildlife -- South Africa -- Mapungubwe Site
- Description: Despite evidence suggesting that road traffic is a major threat to biodiversity loss, very little is known about its actual impact on wildlife populations in South Africa. Globally, road density and traffic volumes are increasing, and although huge budgets are devoted to the construction and upgrading of roads, there is little or no allocation to mitigation measures for protecting fauna in most countries, particularly Africa. Further, no global standardised protocol exists for the rapid assessment of roadkill or the most economical and efficient approach for assessing roadkill rates. Using vehicle field trials, the reliability of detecting artificially deployed roadkill was assessed. Roadkill detection rates decreased significantly at speeds >50 km/h and were also significantly influenced by light conditions (i.e. detection success was greater when the sun was high) and the position of the roadkill on the road (i.e. smaller roadkill on verges were often missed). These results suggest that roadkill sampling was most effective between 1.5 hours after dawn and 1.5 hours before dusk and that driving at slower speeds (<50 km.h⁻ₑ) was required to detect roadkill. This protocol was implemented across three ecological seasons on a 100 km paved road and a 20 km unpaved road in the Greater Mapungubwe Transfrontier Conservation Area, Limpopo Province, South Africa. Driven daily over a 120-day period (three periods consisting each of 40 days), a total of 1,027 roadkill were recorded. These comprised 162 species from all terrestrial vertebrate groups with birds being the most commonly encountered roadkill (50% of all incidents). The high numbers of vertebrates identified as roadkill suggests that road traffic could have potentially unsustainable impacts on wildlife populations and hence the biodiversity of the area. Seventeen variables were identified as possible determinants of roadkill occurrence with season, rainfall, minimum and maximum temperature, habitat type, grass height, grass density, fence type and vehicle type significantly influencing roadkill numbers. Significantly more roadkill were detected on the paved road (9.91/100km) than on the unpaved road (1.8/100km) probably because of greater traffic volumes and the increased speed that vehicles travelled on the paved road. Warmer temperatures and increased rainfall in the preceding 24 hours also increased road mortality numbers as animals tended to become more active during these times. Interestingly, more roadkill was detected in open roadside habitats compared to dense roadside habitats on both the paved and unpaved roads and when grass on the roadside verge was of intermediate height. Open habitat possibly may provide a natural corridor for wildlife which ultimately end up on the road. Roadkill numbers increased when certain other physical barriers, such as cattle fences, were present, probably because these barriers were more penetrable than electric fencing. A series of mitigation measures are proposed to reduce the impacts of roads on wildlife in South Africa. These mitigation measures highlight the need to address the balance between the development of a country’s transport infrastructure and the conservation of its fauna. It is important that research on the impacts of roads becomes standardised to enable robust statistical comparisons which will provide a greater understanding of the potential threats to vertebrate biodiversity
- Full Text:
- Date Issued: 2013
- Authors: Collinson, Wendy Jane
- Date: 2013
- Subjects: Roadkill -- South Africa -- Mapungubwe Site , Traffic safety and wildlife -- South Africa -- Mapungubwe Site
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5606 , http://hdl.handle.net/10962/d1002056 , Roadkill -- South Africa -- Mapungubwe Site , Traffic safety and wildlife -- South Africa -- Mapungubwe Site
- Description: Despite evidence suggesting that road traffic is a major threat to biodiversity loss, very little is known about its actual impact on wildlife populations in South Africa. Globally, road density and traffic volumes are increasing, and although huge budgets are devoted to the construction and upgrading of roads, there is little or no allocation to mitigation measures for protecting fauna in most countries, particularly Africa. Further, no global standardised protocol exists for the rapid assessment of roadkill or the most economical and efficient approach for assessing roadkill rates. Using vehicle field trials, the reliability of detecting artificially deployed roadkill was assessed. Roadkill detection rates decreased significantly at speeds >50 km/h and were also significantly influenced by light conditions (i.e. detection success was greater when the sun was high) and the position of the roadkill on the road (i.e. smaller roadkill on verges were often missed). These results suggest that roadkill sampling was most effective between 1.5 hours after dawn and 1.5 hours before dusk and that driving at slower speeds (<50 km.h⁻ₑ) was required to detect roadkill. This protocol was implemented across three ecological seasons on a 100 km paved road and a 20 km unpaved road in the Greater Mapungubwe Transfrontier Conservation Area, Limpopo Province, South Africa. Driven daily over a 120-day period (three periods consisting each of 40 days), a total of 1,027 roadkill were recorded. These comprised 162 species from all terrestrial vertebrate groups with birds being the most commonly encountered roadkill (50% of all incidents). The high numbers of vertebrates identified as roadkill suggests that road traffic could have potentially unsustainable impacts on wildlife populations and hence the biodiversity of the area. Seventeen variables were identified as possible determinants of roadkill occurrence with season, rainfall, minimum and maximum temperature, habitat type, grass height, grass density, fence type and vehicle type significantly influencing roadkill numbers. Significantly more roadkill were detected on the paved road (9.91/100km) than on the unpaved road (1.8/100km) probably because of greater traffic volumes and the increased speed that vehicles travelled on the paved road. Warmer temperatures and increased rainfall in the preceding 24 hours also increased road mortality numbers as animals tended to become more active during these times. Interestingly, more roadkill was detected in open roadside habitats compared to dense roadside habitats on both the paved and unpaved roads and when grass on the roadside verge was of intermediate height. Open habitat possibly may provide a natural corridor for wildlife which ultimately end up on the road. Roadkill numbers increased when certain other physical barriers, such as cattle fences, were present, probably because these barriers were more penetrable than electric fencing. A series of mitigation measures are proposed to reduce the impacts of roads on wildlife in South Africa. These mitigation measures highlight the need to address the balance between the development of a country’s transport infrastructure and the conservation of its fauna. It is important that research on the impacts of roads becomes standardised to enable robust statistical comparisons which will provide a greater understanding of the potential threats to vertebrate biodiversity
- Full Text:
- Date Issued: 2013
Entomopathogenic fungi for control of soil-borne life stages of false codling moth, Thaumatotibia leucotreta (Meyrick) (1912) (Lepidoptera: Tortricidae)
- Authors: Coombes, Candice Anne
- Date: 2013
- Subjects: Tortricidae , Lepidoptera , Cryptophlebia leucotreta , Insect pests -- Biological control -- South Africa -- Eastern Cape , Tortricidae -- Biological control -- South Africa -- Eastern Cape , Citrus -- Diseases and pests -- Biological control -- South Africa -- Eastern Cape , Entomopathogenic fungi , Fungi as biological pest control agents , Biological pest control agents
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5607 , http://hdl.handle.net/10962/d1002057 , Tortricidae , Lepidoptera , Cryptophlebia leucotreta , Insect pests -- Biological control -- South Africa -- Eastern Cape , Tortricidae -- Biological control -- South Africa -- Eastern Cape , Citrus -- Diseases and pests -- Biological control -- South Africa -- Eastern Cape , Entomopathogenic fungi , Fungi as biological pest control agents , Biological pest control agents
- Description: False codling moth (FCM), Thaumatotibia leucotreta is an extremely important pest of citrus in South Africa and with the shift away from the use of chemicals, alternate control options are needed. One avenue of control which has only recently been investigated against the soil-borne life stages of FCM is the use of entomopathogenic fungi (EPF). In 2009, 12 entomopathogenic fungal isolates collected from South African citrus orchards showed good control potential during laboratory conducted bioassays. The aim of this study was to further analyse the potential of these isolates through concentration-dose and exposure-time response bioassays. After initial re-screening, concentration-dose response and exposure-time response sandconidial bioassays, three isolates were identified as exhibiting the greatest control potential against FCM in soil, Metarhizium anisopliae var. anisopliae (G 11 3 L6 and FCM Ar 23 B3) and Beauveria bassiana (G Ar 17 B3). Percentage mycosis was found to be directly related to fungal concentration as well as the amount of time FCM 5th instar larvae were exposed to the fungal conidia. LC50 values for the three isolates were not greater than 1.92 x 10⁶ conidia.ml⁻ₑ and at the LC₅₀, FCM 5th instar larvae would need to be exposed to the fungus for a maximum of 13 days to ensure a high mortality level. These isolates along with two commercially available EPF products were subjected to field persistence trials whereby net bags filled with a mixture of autoclaved sand and formulated fungal product were buried in an Eastern Cape citrus orchard. The viability of each isolate was measured on a monthly basis for a period of six months. All isolates were capable of persisting in the soil for six months with the collected isolates persisting far better than the commercially used isolates. Two of the isolates, G 11 3 L6 and G Ar 17 B3, were subjected to small scale laboratory application trials. Two formulations were investigated at two concentrations. For each isolate, each formulation and each concentration, FCM 5th instar larvae were applied and allowed to burrow into the soil to pupate before fungal application or after fungal application. Contact between fungi and FCM host is essential as, in contrast to pre-larval treatments, percentage mortality in post-larval treatments was low for both formulations and both isolates. For isolate G Ar 17 B3, a conidial suspension applied as a spray at a concentration of 1 x 10⁷ conidia.ml⁻ₑ obtained the highest percentage mortality (80 %). For isolate G 11 3 L6 however, both formulations performed equally well at a high, 1 x10⁷ conidia.ml⁻ₑ concentration (conidial suspension: 60 %; granular: 65 %) The results obtained thus far are promising for the control of FCM in citrus, but if these EPFs are to successfully integrate into current FCM control practices more research, some of which is discussed, is essential
- Full Text:
- Date Issued: 2013
- Authors: Coombes, Candice Anne
- Date: 2013
- Subjects: Tortricidae , Lepidoptera , Cryptophlebia leucotreta , Insect pests -- Biological control -- South Africa -- Eastern Cape , Tortricidae -- Biological control -- South Africa -- Eastern Cape , Citrus -- Diseases and pests -- Biological control -- South Africa -- Eastern Cape , Entomopathogenic fungi , Fungi as biological pest control agents , Biological pest control agents
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5607 , http://hdl.handle.net/10962/d1002057 , Tortricidae , Lepidoptera , Cryptophlebia leucotreta , Insect pests -- Biological control -- South Africa -- Eastern Cape , Tortricidae -- Biological control -- South Africa -- Eastern Cape , Citrus -- Diseases and pests -- Biological control -- South Africa -- Eastern Cape , Entomopathogenic fungi , Fungi as biological pest control agents , Biological pest control agents
- Description: False codling moth (FCM), Thaumatotibia leucotreta is an extremely important pest of citrus in South Africa and with the shift away from the use of chemicals, alternate control options are needed. One avenue of control which has only recently been investigated against the soil-borne life stages of FCM is the use of entomopathogenic fungi (EPF). In 2009, 12 entomopathogenic fungal isolates collected from South African citrus orchards showed good control potential during laboratory conducted bioassays. The aim of this study was to further analyse the potential of these isolates through concentration-dose and exposure-time response bioassays. After initial re-screening, concentration-dose response and exposure-time response sandconidial bioassays, three isolates were identified as exhibiting the greatest control potential against FCM in soil, Metarhizium anisopliae var. anisopliae (G 11 3 L6 and FCM Ar 23 B3) and Beauveria bassiana (G Ar 17 B3). Percentage mycosis was found to be directly related to fungal concentration as well as the amount of time FCM 5th instar larvae were exposed to the fungal conidia. LC50 values for the three isolates were not greater than 1.92 x 10⁶ conidia.ml⁻ₑ and at the LC₅₀, FCM 5th instar larvae would need to be exposed to the fungus for a maximum of 13 days to ensure a high mortality level. These isolates along with two commercially available EPF products were subjected to field persistence trials whereby net bags filled with a mixture of autoclaved sand and formulated fungal product were buried in an Eastern Cape citrus orchard. The viability of each isolate was measured on a monthly basis for a period of six months. All isolates were capable of persisting in the soil for six months with the collected isolates persisting far better than the commercially used isolates. Two of the isolates, G 11 3 L6 and G Ar 17 B3, were subjected to small scale laboratory application trials. Two formulations were investigated at two concentrations. For each isolate, each formulation and each concentration, FCM 5th instar larvae were applied and allowed to burrow into the soil to pupate before fungal application or after fungal application. Contact between fungi and FCM host is essential as, in contrast to pre-larval treatments, percentage mortality in post-larval treatments was low for both formulations and both isolates. For isolate G Ar 17 B3, a conidial suspension applied as a spray at a concentration of 1 x 10⁷ conidia.ml⁻ₑ obtained the highest percentage mortality (80 %). For isolate G 11 3 L6 however, both formulations performed equally well at a high, 1 x10⁷ conidia.ml⁻ₑ concentration (conidial suspension: 60 %; granular: 65 %) The results obtained thus far are promising for the control of FCM in citrus, but if these EPFs are to successfully integrate into current FCM control practices more research, some of which is discussed, is essential
- Full Text:
- Date Issued: 2013
A Comparative study of two copper(II) based metal-organic frameworks : Cu2¼(OH)½B4C•8H2O and Cu2Na(OH)B4C•7H2O
- Authors: Coombes, Matthew
- Date: 2013
- Subjects: Copper , Organometallic compounds , Supramolecular organometallic chemistry
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4533 , http://hdl.handle.net/10962/d1016245
- Description: This study focussed on two copper(II)-containing metal-organic frameworks (MOFs): Cu2Na(OH)B4C•7H2O and Cu2¼(OH)½B4C•8H2O (B4C = 1,2,4,5- benzenetetracarboxylate). They are both covalent, three-dimensional metalorganic framework polymers containing voids filled with water molecules. Both were characterised by elemental analysis, infrared spectroscopy, X-ray powder diffractometry (both in situ and regular), thermogravimetric analysis, differential scanning calorimetry and X-ray photoelectron spectroscopy. These two MOFs are essentially identical, with the only difference being the substitution of sodium by copper at every 4th site (disordered throughout the crystal). The guest inclusion properties of both MOFs were studied and compared. Although both structures collapse on dehydration, it was observed that Cu2Na(OH)B4C•7H2O is able to take up signifcant amounts of water, methanol and ethanol. All these processes are fully reversible. Car-Parrinello molecular dynamics studies suggest that it is a strong interaction between the oxygen atoms on these molecules with the sodium cation of the MOF that is responsible for this signifcant uptake. In contrast, Cu2¼ (OH)½ B4C•8H2O, the MOF without a sodium cation, did not demonstrate any methanol or ethanol uptake, but was able to take up some water. The uptake of water, however, is not a fully reversible process. The absence of sodium likely results in insuffcient energy to draw methanol and ethanol into the framework, while a subtle rotation of a carboxylate group on dehydration decreases the ability of the framework to form hydrogen bonds, thus reducing the ability to take up water. A series of hydrothermal syntheses were performed in order to develop a method of synthesis superior to the current gel-based synthesis that requires several months and has poor yields. The hydrothermal products were characterized by elemental analysis, infrared spectroscopy, X-ray powder diffractometry, thermogravimetric analysis and differential scanning calorimetry. It was shown that the MOF Cu2Na(OH)B4C•7H2O may be synthesised in almost 100% yield by using a temperature of 120°C over a period of 72 hours. It was not possible to synthesise Cu2¼ (OH)½ B4C•8H2O in a 100% yield - it was only obtained as a minor product.
- Full Text:
- Date Issued: 2013
- Authors: Coombes, Matthew
- Date: 2013
- Subjects: Copper , Organometallic compounds , Supramolecular organometallic chemistry
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4533 , http://hdl.handle.net/10962/d1016245
- Description: This study focussed on two copper(II)-containing metal-organic frameworks (MOFs): Cu2Na(OH)B4C•7H2O and Cu2¼(OH)½B4C•8H2O (B4C = 1,2,4,5- benzenetetracarboxylate). They are both covalent, three-dimensional metalorganic framework polymers containing voids filled with water molecules. Both were characterised by elemental analysis, infrared spectroscopy, X-ray powder diffractometry (both in situ and regular), thermogravimetric analysis, differential scanning calorimetry and X-ray photoelectron spectroscopy. These two MOFs are essentially identical, with the only difference being the substitution of sodium by copper at every 4th site (disordered throughout the crystal). The guest inclusion properties of both MOFs were studied and compared. Although both structures collapse on dehydration, it was observed that Cu2Na(OH)B4C•7H2O is able to take up signifcant amounts of water, methanol and ethanol. All these processes are fully reversible. Car-Parrinello molecular dynamics studies suggest that it is a strong interaction between the oxygen atoms on these molecules with the sodium cation of the MOF that is responsible for this signifcant uptake. In contrast, Cu2¼ (OH)½ B4C•8H2O, the MOF without a sodium cation, did not demonstrate any methanol or ethanol uptake, but was able to take up some water. The uptake of water, however, is not a fully reversible process. The absence of sodium likely results in insuffcient energy to draw methanol and ethanol into the framework, while a subtle rotation of a carboxylate group on dehydration decreases the ability of the framework to form hydrogen bonds, thus reducing the ability to take up water. A series of hydrothermal syntheses were performed in order to develop a method of synthesis superior to the current gel-based synthesis that requires several months and has poor yields. The hydrothermal products were characterized by elemental analysis, infrared spectroscopy, X-ray powder diffractometry, thermogravimetric analysis and differential scanning calorimetry. It was shown that the MOF Cu2Na(OH)B4C•7H2O may be synthesised in almost 100% yield by using a temperature of 120°C over a period of 72 hours. It was not possible to synthesise Cu2¼ (OH)½ B4C•8H2O in a 100% yield - it was only obtained as a minor product.
- Full Text:
- Date Issued: 2013