Ultra-high precision manufacturing
- Authors: Abou-El-Hossein, Khaled
- Subjects: Machining , Diamond turning , f-sa
- Language: English
- Type: text , Lectures
- Identifier: http://hdl.handle.net/10948/20878 , vital:29410
- Description: One of the engineering areas focusing on the research and development of highvalue components and manufacturing technologies is precision engineering. Precision engineering represents a variety of engineering and science disciplines ranging from areas such as mechanical, electronics and industrial engineering to chemistry, physics, optics and materials science. This paper aims at familiarising the reader with the recent advances in ultra-high precision manufacturing technologies and their applications for the production of various critical components employed in different sectors of the industry. In this paper, the principles of ultra-high precision manufacturing will be discussed followed by examples of its use in various industrial applications. The status of ultra-high precision manufacturing in terms of current research issues and future trends will be discussed. In addition, research activities and projects in the area of precision manufacturing that are currently conducted at the NMMU will be also highlighted. Finally, the author looks forward to presenting herewith comprehensive information that could be useful to the reader and easy to understand by the bigger NMMU’s community.
- Full Text:
- Authors: Abou-El-Hossein, Khaled
- Subjects: Machining , Diamond turning , f-sa
- Language: English
- Type: text , Lectures
- Identifier: http://hdl.handle.net/10948/20878 , vital:29410
- Description: One of the engineering areas focusing on the research and development of highvalue components and manufacturing technologies is precision engineering. Precision engineering represents a variety of engineering and science disciplines ranging from areas such as mechanical, electronics and industrial engineering to chemistry, physics, optics and materials science. This paper aims at familiarising the reader with the recent advances in ultra-high precision manufacturing technologies and their applications for the production of various critical components employed in different sectors of the industry. In this paper, the principles of ultra-high precision manufacturing will be discussed followed by examples of its use in various industrial applications. The status of ultra-high precision manufacturing in terms of current research issues and future trends will be discussed. In addition, research activities and projects in the area of precision manufacturing that are currently conducted at the NMMU will be also highlighted. Finally, the author looks forward to presenting herewith comprehensive information that could be useful to the reader and easy to understand by the bigger NMMU’s community.
- Full Text:
Isolation, purification and kinetic characterization of prolyl endopeptidase from Titicum aestivum
- Authors: Abrahams, Adriam Mark
- Date: 2013
- Subjects: Endopeptidases , Wheat , Chemical kinetics , Pharmacokinetics
- Language: English
- Type: Thesis , Masters , MSc (Biochemistry)
- Identifier: vital:11269 , http://hdl.handle.net/10353/d1004356 , Endopeptidases , Wheat , Chemical kinetics , Pharmacokinetics
- Description: PEP activity has been described in several locations and has mostly been linked to a variety of neurological disorders such as schizophrenia, amnaesia, depression as well as other disease states such as anorexia nervosa, bulimia nervosa and blood pressure regulation. The enzyme has also been previously isolated from a variety of archae, microorganisms and several eukaryotic species but no prolyl endopeptidases have been isolated from plants. Plants have high levels of proline and glutamine rich peptides in seeds. We therefore hypothesize plants must express PEPs during germination. Bioinformatics tools were used to identify known PEPs and putative plant PEPs. A global sequence alignment of putative plant PEPs and other known PEPs indicated that the active site amino acids Ser, His and Asp are conserved in putative plant PEP sequences. Furthermore, putative plant PEPs showed similar secondary structures to known PEPs and when a rice PEP was modelled onto porcine brain PEP structure, a high degree of similarity was found. Germination studies of wheat seed showed an increase of PEP activity over time with maximum PEP activity reached after 4 days of germination, which remained at this level until 9 days of germination, implying a function for PEP in plant seed germination. Wheat PEP was purified using ion exchange and gel filtration chromatography with a final yield of less than 1 percent and a relative purity (only 2 bands detected by SDS-PAGE). The purified wheat PEP had a molecular weight of approximately 55kDa, substrate specificity for chymotrypsin-like substrates (N-Suc-Ala-Ala-Pro-Phe-pNa, Km value of 0.58 mM, Kcat of 29.37 s–1; Kcat /Km 50813.14s–1 M–1); a pH optimum of 7.9; temperature optima of 37oC and a high sensitivity to temperature as indicated by loss of activity at temperatures above 40oC. Inhibition studies using E64, Leupeptin and PMSF confirmed that the wheat PEP is from the Serine protease family and is most likely a trypsin-like protease.
- Full Text:
- Date Issued: 2013
- Authors: Abrahams, Adriam Mark
- Date: 2013
- Subjects: Endopeptidases , Wheat , Chemical kinetics , Pharmacokinetics
- Language: English
- Type: Thesis , Masters , MSc (Biochemistry)
- Identifier: vital:11269 , http://hdl.handle.net/10353/d1004356 , Endopeptidases , Wheat , Chemical kinetics , Pharmacokinetics
- Description: PEP activity has been described in several locations and has mostly been linked to a variety of neurological disorders such as schizophrenia, amnaesia, depression as well as other disease states such as anorexia nervosa, bulimia nervosa and blood pressure regulation. The enzyme has also been previously isolated from a variety of archae, microorganisms and several eukaryotic species but no prolyl endopeptidases have been isolated from plants. Plants have high levels of proline and glutamine rich peptides in seeds. We therefore hypothesize plants must express PEPs during germination. Bioinformatics tools were used to identify known PEPs and putative plant PEPs. A global sequence alignment of putative plant PEPs and other known PEPs indicated that the active site amino acids Ser, His and Asp are conserved in putative plant PEP sequences. Furthermore, putative plant PEPs showed similar secondary structures to known PEPs and when a rice PEP was modelled onto porcine brain PEP structure, a high degree of similarity was found. Germination studies of wheat seed showed an increase of PEP activity over time with maximum PEP activity reached after 4 days of germination, which remained at this level until 9 days of germination, implying a function for PEP in plant seed germination. Wheat PEP was purified using ion exchange and gel filtration chromatography with a final yield of less than 1 percent and a relative purity (only 2 bands detected by SDS-PAGE). The purified wheat PEP had a molecular weight of approximately 55kDa, substrate specificity for chymotrypsin-like substrates (N-Suc-Ala-Ala-Pro-Phe-pNa, Km value of 0.58 mM, Kcat of 29.37 s–1; Kcat /Km 50813.14s–1 M–1); a pH optimum of 7.9; temperature optima of 37oC and a high sensitivity to temperature as indicated by loss of activity at temperatures above 40oC. Inhibition studies using E64, Leupeptin and PMSF confirmed that the wheat PEP is from the Serine protease family and is most likely a trypsin-like protease.
- Full Text:
- Date Issued: 2013
Possibilities for multilingualism: a critical case study with selected financial information systems (FIS) students
- Authors: Adams, Natalie
- Date: 2013
- Subjects: Language and languages -- Study and teaching -- Bilingual method , Education, Bilingual
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:8367 , http://hdl.handle.net/10948/d1011052 , Language and languages -- Study and teaching -- Bilingual method , Education, Bilingual
- Description: This study aims at eliciting what shifts in term of multilingual possibilities can be detected in the language attitudes of first year National Diploma in Financial Information Systems students and their lectures. Qualitative methods are used: data is gathered using a questionnaire, 3 focus group discussions with students, interviews with 2 lecturers as well as an interview with language development practitioner. The questionnaire is modelled on attitudinal studies conducted at University of the Western Cape (Dyers 2001), University of Ford Hare (Dalwit 2001) and at Rhodes University (Aziakpono 2008). The questionnaire data, however, is only used a precursor to the study. The focuses of the study are the 3 focus group discussion and the semi structured interview with two lecturers and language development practitioner. The results of the snap shot questionnaire and first focus group discussion are similar to the three studies on language choices at Fort Hare, Rhodes and the University of the Western Cape respectively whereby students prefer English as the language of learning and teaching. The second focus group discussion is based on an article which students had to read. The article, Here, mother tongue clashes with her mother‟s tongue, focuses on the price that South African black children will pay for the constant erosion of African languages. The article‟s provocative focus challenges commonplace acceptance of English and so resonated with the students‟ exploration of multilingual possibilities. Focus group three revealed that students had experienced an attitudinal shift and realized the significance of mother tongue education as well as the importance of multilingual strategies.
- Full Text:
- Date Issued: 2013
- Authors: Adams, Natalie
- Date: 2013
- Subjects: Language and languages -- Study and teaching -- Bilingual method , Education, Bilingual
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:8367 , http://hdl.handle.net/10948/d1011052 , Language and languages -- Study and teaching -- Bilingual method , Education, Bilingual
- Description: This study aims at eliciting what shifts in term of multilingual possibilities can be detected in the language attitudes of first year National Diploma in Financial Information Systems students and their lectures. Qualitative methods are used: data is gathered using a questionnaire, 3 focus group discussions with students, interviews with 2 lecturers as well as an interview with language development practitioner. The questionnaire is modelled on attitudinal studies conducted at University of the Western Cape (Dyers 2001), University of Ford Hare (Dalwit 2001) and at Rhodes University (Aziakpono 2008). The questionnaire data, however, is only used a precursor to the study. The focuses of the study are the 3 focus group discussion and the semi structured interview with two lecturers and language development practitioner. The results of the snap shot questionnaire and first focus group discussion are similar to the three studies on language choices at Fort Hare, Rhodes and the University of the Western Cape respectively whereby students prefer English as the language of learning and teaching. The second focus group discussion is based on an article which students had to read. The article, Here, mother tongue clashes with her mother‟s tongue, focuses on the price that South African black children will pay for the constant erosion of African languages. The article‟s provocative focus challenges commonplace acceptance of English and so resonated with the students‟ exploration of multilingual possibilities. Focus group three revealed that students had experienced an attitudinal shift and realized the significance of mother tongue education as well as the importance of multilingual strategies.
- Full Text:
- Date Issued: 2013
Comparative phytochemical analyses of Aloe Ferox Mill. found in Eastern and Western Cape provinces in South Africa
- Authors: Adams, Zanele
- Date: 2013
- Subjects: Phytochemicals -- South Africa , Medicinal plants -- South Africa , Botanical chemistry -- South Africa , Aloe -- Analysis , Aloe -- Research -- South Africa , Aloin
- Language: English
- Type: Thesis , Masters , MSc (Botany)
- Identifier: vital:11308 , http://hdl.handle.net/10353/d1013114 , Phytochemicals -- South Africa , Medicinal plants -- South Africa , Botanical chemistry -- South Africa , Aloe -- Analysis , Aloe -- Research -- South Africa , Aloin
- Full Text:
- Date Issued: 2013
- Authors: Adams, Zanele
- Date: 2013
- Subjects: Phytochemicals -- South Africa , Medicinal plants -- South Africa , Botanical chemistry -- South Africa , Aloe -- Analysis , Aloe -- Research -- South Africa , Aloin
- Language: English
- Type: Thesis , Masters , MSc (Botany)
- Identifier: vital:11308 , http://hdl.handle.net/10353/d1013114 , Phytochemicals -- South Africa , Medicinal plants -- South Africa , Botanical chemistry -- South Africa , Aloe -- Analysis , Aloe -- Research -- South Africa , Aloin
- Full Text:
- Date Issued: 2013
An exploration of leadership practices: a case study in a public high school in Nigeria
- Authors: Adediji, John Oluwole
- Date: 2013
- Subjects: Educational leadership -- Nigeria -- Case studies Teacher participation in administration -- Nigeria -- Case studies School management and organization -- Nigeria -- Case studies Education, Secondary -- Nigeria
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1405 , http://hdl.handle.net/10962/d1001955
- Description: The management of Nigeria high schools are noted for administrative practices in the management of their schools; hence the term administration is commonly used in their daily operations. This fact on ‘administration’ was emphasised by the Nigerian government in the National Policy on Education (Nigeria, 1981, p. 21). Therefor as a researcher from Nigeria, my rationale for embarking on this research study was to find out to what extent a public high school in Nigeria was still operating in a hierarchical, individualistic, authoritarian style of leadership or whether it has started embracing contemporary approaches such as distributed leadership. The main goal of this study was to explore leadership practices in the case study school with the main focus on how different people relate to each other in the various leadership practices of the school, such as staff and briefing meetings of the school. In addition, my research questions aimed at exploring the respondents’ perceptions of leadership and factors enabling or constraining the distribution of leadership in the school. The study is located within the interpretive paradigm. As a researcher in a wheelchair studying in South Africa I needed to find alternative ways of accessing the research site and gathering data. I was able to use electronic communication for the collection of my data. I used four different tools of data collection methods namely document analysis, observation, questionnaire and stimulated recall interviews. Findings from the study indicated that there was limited evidence of contemporary leadership approaches in the case study school. The school was still operating traditional leadership, while school activities were dominated by a hierarchical chain of command. What emerged from the leadership practices of the school could be termed authorised distributed leadership which was under the command of the school principal. Data also indicated that there were some forms of restricted teacher leadership in the management and administration of the school. In addition, findings revealed that the case study school was very good at the management and administrative functions. The school was very effective and efficient in the controlling and management of both human and material resources. Lastly, findings from the case study school indicated some enabling factors to the distribution of leadership in the case study school which include a culture of respect and cordial relations among the SMT and the teachers, Prominent among constraining factors to the distribution of leadership in the case study school were: cultural orientation of the people where the case school was located, exclusionary religious practices by the principal of the school and the inhibiting role played by the Ministry of Education. Finally, based on these findings, recommendations were made both for practice and for future research.
- Full Text:
- Date Issued: 2013
- Authors: Adediji, John Oluwole
- Date: 2013
- Subjects: Educational leadership -- Nigeria -- Case studies Teacher participation in administration -- Nigeria -- Case studies School management and organization -- Nigeria -- Case studies Education, Secondary -- Nigeria
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1405 , http://hdl.handle.net/10962/d1001955
- Description: The management of Nigeria high schools are noted for administrative practices in the management of their schools; hence the term administration is commonly used in their daily operations. This fact on ‘administration’ was emphasised by the Nigerian government in the National Policy on Education (Nigeria, 1981, p. 21). Therefor as a researcher from Nigeria, my rationale for embarking on this research study was to find out to what extent a public high school in Nigeria was still operating in a hierarchical, individualistic, authoritarian style of leadership or whether it has started embracing contemporary approaches such as distributed leadership. The main goal of this study was to explore leadership practices in the case study school with the main focus on how different people relate to each other in the various leadership practices of the school, such as staff and briefing meetings of the school. In addition, my research questions aimed at exploring the respondents’ perceptions of leadership and factors enabling or constraining the distribution of leadership in the school. The study is located within the interpretive paradigm. As a researcher in a wheelchair studying in South Africa I needed to find alternative ways of accessing the research site and gathering data. I was able to use electronic communication for the collection of my data. I used four different tools of data collection methods namely document analysis, observation, questionnaire and stimulated recall interviews. Findings from the study indicated that there was limited evidence of contemporary leadership approaches in the case study school. The school was still operating traditional leadership, while school activities were dominated by a hierarchical chain of command. What emerged from the leadership practices of the school could be termed authorised distributed leadership which was under the command of the school principal. Data also indicated that there were some forms of restricted teacher leadership in the management and administration of the school. In addition, findings revealed that the case study school was very good at the management and administrative functions. The school was very effective and efficient in the controlling and management of both human and material resources. Lastly, findings from the case study school indicated some enabling factors to the distribution of leadership in the case study school which include a culture of respect and cordial relations among the SMT and the teachers, Prominent among constraining factors to the distribution of leadership in the case study school were: cultural orientation of the people where the case school was located, exclusionary religious practices by the principal of the school and the inhibiting role played by the Ministry of Education. Finally, based on these findings, recommendations were made both for practice and for future research.
- Full Text:
- Date Issued: 2013
Nanoconjugates of CdTe@ ZnS quantum dots with cobalt tetraamino-phthalocyanine
- Adegoke, Oluwasesan, Antunes, Edith M, Nyokong, Tebello
- Authors: Adegoke, Oluwasesan , Antunes, Edith M , Nyokong, Tebello
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/242049 , vital:50996 , xlink:href="https://doi.org/10.1016/j.jphotochem.2013.02.010"
- Description: The covalent linking of thiol-capped CdTe@ZnS QDs with cobalt tetraamino-phthalocyanine (CoTAPc) has been successfully carried out. Several techniques such as time-resolved fluorescence measurements, thermal gravimetric analysis,transmissionelectronmicroscopy andspectrophotometric techniques were employed to characterize the nanoconjugates. Covalent binding of the QDs with CoTAPc resulted in the fluorescence quenching of the former. In the presence of varying concentrations of superoxide anion (O2 •−), the fluorescence of the QDs in the conjugate was gradually enhanced and the detection limits obtained were 2.1 and 2.4 nM for the smaller and larger QDs, respectively. Based on the excellent selectivity displayed by the nanoconjugates towards O2 •− over other biologically active species, a potential nanosensor was developed.
- Full Text:
- Date Issued: 2013
- Authors: Adegoke, Oluwasesan , Antunes, Edith M , Nyokong, Tebello
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/242049 , vital:50996 , xlink:href="https://doi.org/10.1016/j.jphotochem.2013.02.010"
- Description: The covalent linking of thiol-capped CdTe@ZnS QDs with cobalt tetraamino-phthalocyanine (CoTAPc) has been successfully carried out. Several techniques such as time-resolved fluorescence measurements, thermal gravimetric analysis,transmissionelectronmicroscopy andspectrophotometric techniques were employed to characterize the nanoconjugates. Covalent binding of the QDs with CoTAPc resulted in the fluorescence quenching of the former. In the presence of varying concentrations of superoxide anion (O2 •−), the fluorescence of the QDs in the conjugate was gradually enhanced and the detection limits obtained were 2.1 and 2.4 nM for the smaller and larger QDs, respectively. Based on the excellent selectivity displayed by the nanoconjugates towards O2 •− over other biologically active species, a potential nanosensor was developed.
- Full Text:
- Date Issued: 2013
Probing the sensitive and selective luminescent detection of peroxynitrite using thiol-capped CdTe and CdTe@ ZnS quantum dots
- Adegoke, Oluwasesan, Nyokong, Tebello
- Authors: Adegoke, Oluwasesan , Nyokong, Tebello
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/193757 , vital:45393 , xlink:href="https://doi.org/10.1016/j.jlumin.2012.08.002"
- Description: CdTe and CdTe@ZnS quantum dots (QDs) capped with 3-mercaptopropionic acid (MPA), thioglycolic acid (TGA), or glutathione (GSH) have been employed for the first time as luminescent probes for the sensitive and selective detection of peroxynitrite (ONOO−) in aqueous solution. The sensitivity of the proposed probe followed the order: MPA–TGA–CdTe@ZnS>GSH–TGA–CdTe@ZnS>MPA–CdTe QDs. The varying degree of quenching is elucidated based on the QD–thiolate bond of CdTe@ZnS being more sensitive to oxidation from ONOO− than CdTe. The selectivity of the probe in the presence of co-existing species followed the order: GSH–TGA–CdTe@ZnS>MPA–TGA–CdTe@ZnS>MPA–CdTe QDs. QDs capped with MPA showed less selectivity for ONOO− than GSH. The best limit of detection (LOD) of 12.6 nM was obtained for MPA–TGA–CdTe@ZnS QDs. Time-resolved fluorescence measurements indicated that the interaction between ONOO− and the QDs is static in nature.
- Full Text:
- Date Issued: 2013
- Authors: Adegoke, Oluwasesan , Nyokong, Tebello
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/193757 , vital:45393 , xlink:href="https://doi.org/10.1016/j.jlumin.2012.08.002"
- Description: CdTe and CdTe@ZnS quantum dots (QDs) capped with 3-mercaptopropionic acid (MPA), thioglycolic acid (TGA), or glutathione (GSH) have been employed for the first time as luminescent probes for the sensitive and selective detection of peroxynitrite (ONOO−) in aqueous solution. The sensitivity of the proposed probe followed the order: MPA–TGA–CdTe@ZnS>GSH–TGA–CdTe@ZnS>MPA–CdTe QDs. The varying degree of quenching is elucidated based on the QD–thiolate bond of CdTe@ZnS being more sensitive to oxidation from ONOO− than CdTe. The selectivity of the probe in the presence of co-existing species followed the order: GSH–TGA–CdTe@ZnS>MPA–TGA–CdTe@ZnS>MPA–CdTe QDs. QDs capped with MPA showed less selectivity for ONOO− than GSH. The best limit of detection (LOD) of 12.6 nM was obtained for MPA–TGA–CdTe@ZnS QDs. Time-resolved fluorescence measurements indicated that the interaction between ONOO− and the QDs is static in nature.
- Full Text:
- Date Issued: 2013
Fluorescence “turn on” probe for bromide ion using nanoconjugates of glutathione-capped CdTe@ ZnS quantum dots with nickel tetraamino-phthalocyanine
- Adegoke, Oluwasesan, Nyokong, Tebello
- Authors: Adegoke, Oluwasesan , Nyokong, Tebello
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/190479 , vital:44998 , xlink:href="https://doi.org/10.1016/j.jphotochem.2013.05.013"
- Description: In this paper, three differently sized glutathione (GSH)-capped CdTe@ZnS quantum dots (QDs) have been successfully conjugated to nickel tetraamino-phthalocyanine (NiTAPc) to form different QDs-NiTAPc nanocomplexes. Several techniques such as TEM, FT-IR, time-resolved fluorescence measurement and electronic spectroscopy were employed to characterize the nanocomplex. Bromide ion was chosen as a model anion to test the efficacy of the nanoprobe. The fluorescence of the nanoconjugate was “turned off” upon binding but was progressively “turned on” upon interaction with varying concentrations of bromide ion. Experimental results showed that the quantum size effect of nanocrystal QD determined the overall sensitivity and selectivity of the nanoprobe and followed the order QD563-NiTAPc > QD605-NiTAPc > QD621-NiTAPc. The mechanism of reaction is proposed.
- Full Text:
- Date Issued: 2013
- Authors: Adegoke, Oluwasesan , Nyokong, Tebello
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/190479 , vital:44998 , xlink:href="https://doi.org/10.1016/j.jphotochem.2013.05.013"
- Description: In this paper, three differently sized glutathione (GSH)-capped CdTe@ZnS quantum dots (QDs) have been successfully conjugated to nickel tetraamino-phthalocyanine (NiTAPc) to form different QDs-NiTAPc nanocomplexes. Several techniques such as TEM, FT-IR, time-resolved fluorescence measurement and electronic spectroscopy were employed to characterize the nanocomplex. Bromide ion was chosen as a model anion to test the efficacy of the nanoprobe. The fluorescence of the nanoconjugate was “turned off” upon binding but was progressively “turned on” upon interaction with varying concentrations of bromide ion. Experimental results showed that the quantum size effect of nanocrystal QD determined the overall sensitivity and selectivity of the nanoprobe and followed the order QD563-NiTAPc > QD605-NiTAPc > QD621-NiTAPc. The mechanism of reaction is proposed.
- Full Text:
- Date Issued: 2013
Fluorescence “switch on” of conjugates of CdTe@ ZnS quantum dots with Al, Ni and Zn tetraamino-phthalocyanines by hydrogen peroxide
- Adegoke, Oluwasesan, Khene, Samson M, Nyokong, Tebello
- Authors: Adegoke, Oluwasesan , Khene, Samson M , Nyokong, Tebello
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/241750 , vital:50966 , xlink:href="https://doi.org/10.1007/s10895-013-1222-x"
- Description: In this study, we have developed a novel nanoprobe for H2O2 based on the conjugation of CdTe@ZnS quantum dots (QDs) to different metal tetraamino-phthalocyanine (MTAPc): (M = (OAc)Al, {OAc = acetate}, Ni and Zn). Chemical coordination of the QDs to the MTAPc resulted in the fluorescence “switch off” of the linked QDs which was associated with Förster resonance energy transfer (FRET). In the presence of varying concentration of H2O2, the fluorescence of the linked QDs was progressively “switched on” and the FRET mechanism between the QDs and the MTAPc was disrupted. The sensitivity/limit of detection of the nanoprobe followed the order: QDs-ZnTAPc (2.2 μM) > QDs-NiTAPc (4.4 μM) > QDs-AlTAPc (9.8 μM) while the selectivity followed the order: QDs-NiTAPc > QDs-AlTAPc > QDs-ZnTAPc. The varying degree of sensitivity/selectivity and mechanism of detection is discussed in detail.
- Full Text:
- Date Issued: 2013
- Authors: Adegoke, Oluwasesan , Khene, Samson M , Nyokong, Tebello
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/241750 , vital:50966 , xlink:href="https://doi.org/10.1007/s10895-013-1222-x"
- Description: In this study, we have developed a novel nanoprobe for H2O2 based on the conjugation of CdTe@ZnS quantum dots (QDs) to different metal tetraamino-phthalocyanine (MTAPc): (M = (OAc)Al, {OAc = acetate}, Ni and Zn). Chemical coordination of the QDs to the MTAPc resulted in the fluorescence “switch off” of the linked QDs which was associated with Förster resonance energy transfer (FRET). In the presence of varying concentration of H2O2, the fluorescence of the linked QDs was progressively “switched on” and the FRET mechanism between the QDs and the MTAPc was disrupted. The sensitivity/limit of detection of the nanoprobe followed the order: QDs-ZnTAPc (2.2 μM) > QDs-NiTAPc (4.4 μM) > QDs-AlTAPc (9.8 μM) while the selectivity followed the order: QDs-NiTAPc > QDs-AlTAPc > QDs-ZnTAPc. The varying degree of sensitivity/selectivity and mechanism of detection is discussed in detail.
- Full Text:
- Date Issued: 2013
Possible futures for the Republic of South Africa towards 2055
- Adendorff, Christian Michael
- Authors: Adendorff, Christian Michael
- Date: 2013
- Subjects: Economic development -- South Africa , Sustainable development -- South Africa
- Language: English
- Type: Thesis , Doctoral , DBA
- Identifier: http://hdl.handle.net/10948/7816 , vital:24294
- Description: The purpose of this thesis was to develop four scenarios for South Africa over the next forty years: Mandela's Dream in which positive elements come into function for South Africa's economy and governance; the Historical African Syndrome, in which the key driving forces unfold in an uneven pattern, or have a differentiated impact on South Africa's economy; the Good, the Bad and the Ugly in which less good governance prevails, but where a fortunate economy and firm national management allow South Africa to become competitive and benefit from satisfactory economic growth; and the Pyramid Syndrome Scenario in which negative regional drivers of change corrode positive policies and initiatives in a manner which compounds the pre-existing threats to South Africa's growth.
- Full Text:
- Date Issued: 2013
- Authors: Adendorff, Christian Michael
- Date: 2013
- Subjects: Economic development -- South Africa , Sustainable development -- South Africa
- Language: English
- Type: Thesis , Doctoral , DBA
- Identifier: http://hdl.handle.net/10948/7816 , vital:24294
- Description: The purpose of this thesis was to develop four scenarios for South Africa over the next forty years: Mandela's Dream in which positive elements come into function for South Africa's economy and governance; the Historical African Syndrome, in which the key driving forces unfold in an uneven pattern, or have a differentiated impact on South Africa's economy; the Good, the Bad and the Ugly in which less good governance prevails, but where a fortunate economy and firm national management allow South Africa to become competitive and benefit from satisfactory economic growth; and the Pyramid Syndrome Scenario in which negative regional drivers of change corrode positive policies and initiatives in a manner which compounds the pre-existing threats to South Africa's growth.
- Full Text:
- Date Issued: 2013
Ethnic conflict in Nigeria: a challenge to inclusive social and political development
- Authors: Adetiba, Toyin Cotties
- Date: 2013
- Subjects: Genocide -- Nigeria , Ethnic conflict -- Nigeria , National characteristics, Nigerian , Citizenship -- Nigeria , Ethnicity -- Nigeria , Multiculturalism -- Nigeria , Economic development -- Nigeria , Nigeria -- Politics and government , Nationalism -- Nigeria , Nigeria -- Social conditions
- Language: English
- Type: Thesis , Doctoral , PhD (Social Science Dev)
- Identifier: vital:11432 , http://hdl.handle.net/10353/d1006955 , Genocide -- Nigeria , Ethnic conflict -- Nigeria , National characteristics, Nigerian , Citizenship -- Nigeria , Ethnicity -- Nigeria , Multiculturalism -- Nigeria , Economic development -- Nigeria , Nigeria -- Politics and government , Nationalism -- Nigeria , Nigeria -- Social conditions
- Description: The question of ethnicity has been one of the most topical subjects of study by social scientists. The controversies around this phenomenon seem to have been heated up by the high visibility of mobilized and politicized ethnic groups in most multi-ethnic states. Therefore, the extent to which ethnic nationalities are able to effectively manage the interplay of ethnic differences determines to what extent a multi-ethnic nation develops without crisis. Historically Nigeria has come a long way from multi-ethnic entity with political differences and background to the amalgamation of 1914 till the present structure of thirty-six states. Ethnicity, no doubt has contributed immensely to ethnic conflicts in Nigeria because of long standing revulsion or resentments towards ethnic groups different from one’s own or fear of domination which can as well lead ethnic groups to resort to violence as a means to protect and preserve the existing ethnic groups. Significantly ethnicity in Nigeria, is a product inequality among the various ethnic groups orchestrated by a long period of colonialism; a period which witnessed the ascendancy of three major ethnic groups to the socio-political domination of other ethnic groups and a period when the three major ethnic groups were used as a pedestal for the distribution of socio-political goods, resulting in the inability of other ethnic groups to access these socio-political goods. This situation has continued to impact negatively on the forces of national integration and cohesion in ethnically divided Nigeria. Considering the relationship between ethnicity and development; socio-political exclusion is not only ethically dangerous to development but also economically unproductive. It deprives groups and individuals of the opportunity for the necessary development that can be beneficial to the society. Thus, it is important to develop an integrative socio-political frame-work that explicitly recognizes the participatory role of every ethnic group in governance. Hence, there is a need for the adoption of inclusive governance to manage ethnicity in Nigeria. Notwithstanding, ethnic conflict still persists and an attempt will be made in this study to identify the reasons. Central to socio-political sustainability in Nigeria is a system that should recognize that differences are important to development and encompass notions of equality. Such a system should acknowledge the socio-political and economic power of every ethnic group and promote a system devoid of ethnocentric and exclusionary socio-political and economic policies.
- Full Text:
- Date Issued: 2013
- Authors: Adetiba, Toyin Cotties
- Date: 2013
- Subjects: Genocide -- Nigeria , Ethnic conflict -- Nigeria , National characteristics, Nigerian , Citizenship -- Nigeria , Ethnicity -- Nigeria , Multiculturalism -- Nigeria , Economic development -- Nigeria , Nigeria -- Politics and government , Nationalism -- Nigeria , Nigeria -- Social conditions
- Language: English
- Type: Thesis , Doctoral , PhD (Social Science Dev)
- Identifier: vital:11432 , http://hdl.handle.net/10353/d1006955 , Genocide -- Nigeria , Ethnic conflict -- Nigeria , National characteristics, Nigerian , Citizenship -- Nigeria , Ethnicity -- Nigeria , Multiculturalism -- Nigeria , Economic development -- Nigeria , Nigeria -- Politics and government , Nationalism -- Nigeria , Nigeria -- Social conditions
- Description: The question of ethnicity has been one of the most topical subjects of study by social scientists. The controversies around this phenomenon seem to have been heated up by the high visibility of mobilized and politicized ethnic groups in most multi-ethnic states. Therefore, the extent to which ethnic nationalities are able to effectively manage the interplay of ethnic differences determines to what extent a multi-ethnic nation develops without crisis. Historically Nigeria has come a long way from multi-ethnic entity with political differences and background to the amalgamation of 1914 till the present structure of thirty-six states. Ethnicity, no doubt has contributed immensely to ethnic conflicts in Nigeria because of long standing revulsion or resentments towards ethnic groups different from one’s own or fear of domination which can as well lead ethnic groups to resort to violence as a means to protect and preserve the existing ethnic groups. Significantly ethnicity in Nigeria, is a product inequality among the various ethnic groups orchestrated by a long period of colonialism; a period which witnessed the ascendancy of three major ethnic groups to the socio-political domination of other ethnic groups and a period when the three major ethnic groups were used as a pedestal for the distribution of socio-political goods, resulting in the inability of other ethnic groups to access these socio-political goods. This situation has continued to impact negatively on the forces of national integration and cohesion in ethnically divided Nigeria. Considering the relationship between ethnicity and development; socio-political exclusion is not only ethically dangerous to development but also economically unproductive. It deprives groups and individuals of the opportunity for the necessary development that can be beneficial to the society. Thus, it is important to develop an integrative socio-political frame-work that explicitly recognizes the participatory role of every ethnic group in governance. Hence, there is a need for the adoption of inclusive governance to manage ethnicity in Nigeria. Notwithstanding, ethnic conflict still persists and an attempt will be made in this study to identify the reasons. Central to socio-political sustainability in Nigeria is a system that should recognize that differences are important to development and encompass notions of equality. Such a system should acknowledge the socio-political and economic power of every ethnic group and promote a system devoid of ethnocentric and exclusionary socio-political and economic policies.
- Full Text:
- Date Issued: 2013
Metal nanoparticles caused death of metastatic MDA-MB-231 breast cancer cells:
- Adeyemi, Oluyomi, Edkins, Adrienne L, Whiteley, Christopher
- Authors: Adeyemi, Oluyomi , Edkins, Adrienne L , Whiteley, Christopher
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/164797 , vital:41173 , DOI: 10.1016/j.toxlet.2013.05.599
- Description: Available data on the toxicity of nanoparticles is a subject of controversy. The interaction of nanoparticles with biological systems including living cells has become one of the most urgent areas of collaborative research in materials science and biology. This is due to the fact that toxicity of nanomaterials are ill defined in terms of cause–effect relationships.
- Full Text:
- Date Issued: 2013
- Authors: Adeyemi, Oluyomi , Edkins, Adrienne L , Whiteley, Christopher
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/164797 , vital:41173 , DOI: 10.1016/j.toxlet.2013.05.599
- Description: Available data on the toxicity of nanoparticles is a subject of controversy. The interaction of nanoparticles with biological systems including living cells has become one of the most urgent areas of collaborative research in materials science and biology. This is due to the fact that toxicity of nanomaterials are ill defined in terms of cause–effect relationships.
- Full Text:
- Date Issued: 2013
Biochemical characterisation of Pfj2, a Plasmodium falciparum heat shock protein 40 chaperone potentially involved in protein quality control in the endoplasmic reticulum
- Authors: Afolayan, Omolola Folasade
- Date: 2013
- Subjects: Plasmodium falciparum Endoplasmic reticulum Heat shock proteins Malaria , Mosquito-borne infectious disease
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3883 , http://hdl.handle.net/10962/d1001617
- Description: Plasmodium falciparum is a protozoan parasite that causes a severe form of malaria, a mosquito-borne infectious disease in humans. P. falciparum encodes a number of proteins to facilitate its life-cycle, including a type II heat shock protein 40 (Hsp40), Pfj2. Pfj2 shows a degree of homology to human ERdj5, a resident protein of the endoplasmic reticulum (ER) that promotes protein quality control by facilitating the degradation of misfolded proteins. The overall aim of this study was to further understand the function of Pfj2 in the P. falciparum cell by characterising it biochemically. A bioinformatic analysis of Pfj2 was carried out to enable the identification of a potential ER signal sequence and cleavage site. Furthermore, an analysis of Pfj2 protein sequence was performed to compare domain similarities and identities with typical type II Hsp40s namely, human ERdj5, S. cerevisiae Sis1, human Hsj1a and human DnaJB4. The method used included the insertion of the codon-optimised coding sequence for the processed ER form of Pfj2 into the prokaryotic expression vector, pQE30, to enable overproduction of a histidine-tagged protein. A 62 kDa His₆-Pfj2 was successfully expressed in Escherichia coli and purified using denaturing nickel affinity chromatography. ATPase assays were performed to determine the ability of His₆- Pfj2 to stimulate the chaperone activity of the ER Hsp70, also called immunoglobulin binding protein (BiP). Initial studies were conducted on readily available mammalian His₆-BiP as a control, which was shown to have an intrinsic activity of 12.07±3.92 nmolPi/min/mg. His₆- Pfj2 did not stimulate the ATPase activity of mammalian His₆-BiP, suggesting that it either could not act as a co-chaperone of mammalian His₆-BiP (specificity), or it required a misfolded substrate in the system. Therefore, ongoing studies are addressing the interaction of Pfj2 and misfolded substrates with P. falciparum BiP. The results of these studies will further our understanding of a poorly-studied parasite chaperone that represents a potential drug target for development of novel strategies for the control of a serious human disease
- Full Text:
- Date Issued: 2013
- Authors: Afolayan, Omolola Folasade
- Date: 2013
- Subjects: Plasmodium falciparum Endoplasmic reticulum Heat shock proteins Malaria , Mosquito-borne infectious disease
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3883 , http://hdl.handle.net/10962/d1001617
- Description: Plasmodium falciparum is a protozoan parasite that causes a severe form of malaria, a mosquito-borne infectious disease in humans. P. falciparum encodes a number of proteins to facilitate its life-cycle, including a type II heat shock protein 40 (Hsp40), Pfj2. Pfj2 shows a degree of homology to human ERdj5, a resident protein of the endoplasmic reticulum (ER) that promotes protein quality control by facilitating the degradation of misfolded proteins. The overall aim of this study was to further understand the function of Pfj2 in the P. falciparum cell by characterising it biochemically. A bioinformatic analysis of Pfj2 was carried out to enable the identification of a potential ER signal sequence and cleavage site. Furthermore, an analysis of Pfj2 protein sequence was performed to compare domain similarities and identities with typical type II Hsp40s namely, human ERdj5, S. cerevisiae Sis1, human Hsj1a and human DnaJB4. The method used included the insertion of the codon-optimised coding sequence for the processed ER form of Pfj2 into the prokaryotic expression vector, pQE30, to enable overproduction of a histidine-tagged protein. A 62 kDa His₆-Pfj2 was successfully expressed in Escherichia coli and purified using denaturing nickel affinity chromatography. ATPase assays were performed to determine the ability of His₆- Pfj2 to stimulate the chaperone activity of the ER Hsp70, also called immunoglobulin binding protein (BiP). Initial studies were conducted on readily available mammalian His₆-BiP as a control, which was shown to have an intrinsic activity of 12.07±3.92 nmolPi/min/mg. His₆- Pfj2 did not stimulate the ATPase activity of mammalian His₆-BiP, suggesting that it either could not act as a co-chaperone of mammalian His₆-BiP (specificity), or it required a misfolded substrate in the system. Therefore, ongoing studies are addressing the interaction of Pfj2 and misfolded substrates with P. falciparum BiP. The results of these studies will further our understanding of a poorly-studied parasite chaperone that represents a potential drug target for development of novel strategies for the control of a serious human disease
- Full Text:
- Date Issued: 2013
An investigation into factors that shape secondary school female retention in two rural public schools, Alimosho Region, Lagos State, Nigeria
- Agbomeji, Ayinda Mojeed Oladele
- Authors: Agbomeji, Ayinda Mojeed Oladele
- Date: 2013
- Subjects: Dropouts -- Prevention -- Nigeria -- Alimosho Local Government Area High school dropouts -- Nigeria -- Alimosho Local Government Area Education, Secondary -- Nigeria -- Alimosho Local Government Area Sex differences in education -- Nigeria -- Alimosho Local Government Area High school girls -- Education -- Nigeria -- Alimosho Local Government Area Student aspirations -- Nigeria -- Alimosho Local Government Area Vocational interests -- Nigeria -- Alimosho Local Government Area Sex discrimination in education -- Nigeria -- Alimosho Local Government Area Vocational guidance -- Nigeria -- Alimosho Local Government Area
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1835 , http://hdl.handle.net/10962/d1004331
- Description: Challenges of access to education in the developing world and elsewhere appear to be widespread. Many declarations and conventions have been developed to assist countries to respond to the issue. While challenges of access are universal, Africa in general and sub-Saharan Africa in particular are presented with additional concerns about gender equality and gender parity. While learner numbers seem to be on the decline globally, dropout amongst girls is disproportionately greater than amongst boys. Even though school retention presents a challenge at all levels of the schooling system, it is more acute for girls at the secondary school level. This study was conducted to examine and understand factors that shape retention of secondary school female learners in two rural public schools in Alimosho Region of Lagos State, Nigeria. The study design was qualitative and interpretive in nature. Data collection strategies included administered questionnaires in two schools, focus group discussion with twenty female learners in two schools, case studies, individual interviews with four participants from two schools, and observation in English and Biology classes where the two teachers from the two schools participated in the interviews. Ethical clearance from Alimosho Educational Region office and the two schools was obtained before undertaking the study. Participants’ school principals also signed written consent forms before interviews. The female learners were briefed about the study interview activities and advised that their participation was voluntary and that they were free to withdraw at any point. This study drew on Sen’s (1989, 2000) capabilities theory to understand the phenomenon beyond dominant discourses on education that view education as a basic human right or that focus on economic and development gains. The key finding of this study is that in-school and out- of- school factors interact in complex ways to support female learner retention. Key among these are value placed on education by female learners and significant others, particularly parents; family support; and individual aspirations. Extra-curricular participation, government policy, role models, and peer support were also found to be important factors that mediate progression and retention.
- Full Text:
- Date Issued: 2013
- Authors: Agbomeji, Ayinda Mojeed Oladele
- Date: 2013
- Subjects: Dropouts -- Prevention -- Nigeria -- Alimosho Local Government Area High school dropouts -- Nigeria -- Alimosho Local Government Area Education, Secondary -- Nigeria -- Alimosho Local Government Area Sex differences in education -- Nigeria -- Alimosho Local Government Area High school girls -- Education -- Nigeria -- Alimosho Local Government Area Student aspirations -- Nigeria -- Alimosho Local Government Area Vocational interests -- Nigeria -- Alimosho Local Government Area Sex discrimination in education -- Nigeria -- Alimosho Local Government Area Vocational guidance -- Nigeria -- Alimosho Local Government Area
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1835 , http://hdl.handle.net/10962/d1004331
- Description: Challenges of access to education in the developing world and elsewhere appear to be widespread. Many declarations and conventions have been developed to assist countries to respond to the issue. While challenges of access are universal, Africa in general and sub-Saharan Africa in particular are presented with additional concerns about gender equality and gender parity. While learner numbers seem to be on the decline globally, dropout amongst girls is disproportionately greater than amongst boys. Even though school retention presents a challenge at all levels of the schooling system, it is more acute for girls at the secondary school level. This study was conducted to examine and understand factors that shape retention of secondary school female learners in two rural public schools in Alimosho Region of Lagos State, Nigeria. The study design was qualitative and interpretive in nature. Data collection strategies included administered questionnaires in two schools, focus group discussion with twenty female learners in two schools, case studies, individual interviews with four participants from two schools, and observation in English and Biology classes where the two teachers from the two schools participated in the interviews. Ethical clearance from Alimosho Educational Region office and the two schools was obtained before undertaking the study. Participants’ school principals also signed written consent forms before interviews. The female learners were briefed about the study interview activities and advised that their participation was voluntary and that they were free to withdraw at any point. This study drew on Sen’s (1989, 2000) capabilities theory to understand the phenomenon beyond dominant discourses on education that view education as a basic human right or that focus on economic and development gains. The key finding of this study is that in-school and out- of- school factors interact in complex ways to support female learner retention. Key among these are value placed on education by female learners and significant others, particularly parents; family support; and individual aspirations. Extra-curricular participation, government policy, role models, and peer support were also found to be important factors that mediate progression and retention.
- Full Text:
- Date Issued: 2013
Statistical model for risk diversification in renewable energy
- Authors: Ahame, Edmund
- Date: 2013
- Subjects: Renewable energy sources -- Statistics , Diversification in industry
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10564 , http://hdl.handle.net/10948/d1008399 , Renewable energy sources -- Statistics , Diversification in industry
- Description: The growth of the industry and population of South Africa urges to seek new sources of electric power, hence the need to look at alternative power sources. Power output from some renewable energy sources is highly volatile. For instance power output from wind turbines or photovoltaic solar panels fluctuates between zero and the maximum rated power out. To optimize the overall power output a model was designed to determine the best trade-off between production from two or more renewable energy sources putting emphasis on wind and solar. Different measures of risk, such as coefficient of variation (CV) and value at risk (VAR), were used to determine the best hybrid renewable energy system (HRES) configuration. Depending on the investors’ expected returns (demand) and risk averseness, they will be able to use the model to choose the best configuration that suites their needs. In general it was found that investing in a diversified HRES is better than investing in individual power sources.
- Full Text:
- Date Issued: 2013
- Authors: Ahame, Edmund
- Date: 2013
- Subjects: Renewable energy sources -- Statistics , Diversification in industry
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:10564 , http://hdl.handle.net/10948/d1008399 , Renewable energy sources -- Statistics , Diversification in industry
- Description: The growth of the industry and population of South Africa urges to seek new sources of electric power, hence the need to look at alternative power sources. Power output from some renewable energy sources is highly volatile. For instance power output from wind turbines or photovoltaic solar panels fluctuates between zero and the maximum rated power out. To optimize the overall power output a model was designed to determine the best trade-off between production from two or more renewable energy sources putting emphasis on wind and solar. Different measures of risk, such as coefficient of variation (CV) and value at risk (VAR), were used to determine the best hybrid renewable energy system (HRES) configuration. Depending on the investors’ expected returns (demand) and risk averseness, they will be able to use the model to choose the best configuration that suites their needs. In general it was found that investing in a diversified HRES is better than investing in individual power sources.
- Full Text:
- Date Issued: 2013
Barriers to public participation in developmental projects : a Nigerian community perspective (case study of the Gbaran-Ubie oil and gas project)
- Authors: Akoh, Emmanuel Inalegwu
- Date: 2013
- Subjects: Gbaran-Ubie oil and gas project -- Public opinion Community development -- Nigeria Economic development -- Nigeria -- Environmental aspects
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:715 , http://hdl.handle.net/10962/d1001869
- Description: This research paper is broken up into three sections, namely an evaluation report, a literature review and research methodology. The evaluation report section is the assessment of the current state of change readiness within the Eskom Contact Centre’s based upon what the literature on change readiness prescribes. The timing of the change readiness assessment is just prior to significant organisational change. The organisational change that was about to be initiated by the organisation was initiated from the boardroom of the most senior echelons of the organisation, and was directed in a top down approach, being a strategic organisational change. The change is deemed to be critical to the organisation being able to meet its long term strategic and sustainability objectives. A critical examination of the literature explored the meaning of change readiness, the importance of it and explained the consequences for organisations that commit to transformational agendas without being ready. Key concepts such as such resistance to change and organisational inertia are described and differentiated from change readiness. The ADKAR change model and its change readiness assessment instrument were used due to the organisations preference for the model. The ADKAR model formed the framework for the analyses of the data, the discussion of the results and the recommendations to the organisation. The research conducted was quantitative in nature; a questionnaire was distributed to the employees of the seven Eskom Contact centre sites around the country through an email. A slightly modified version of the ADKAR change readiness questionnaire was sent via email with an on-line questionnaire link on it; and questions on individual readiness for change were used to assess the level of readiness of the employees. Most of the descriptive and inferential statistics were analysed with the use of Excel (version, 2010), with Factor Analysis being done in Statistica. The results of the research showed that: [iii] - The factors as proposed by the ADKAR change readiness assessment questionnaire (i.e. Awareness, Desire, Knowledge, Ability and Reinforcement) are not different enough to be considered as independent factors for this data set. Based on factor analysis, the factors were subsequently amended from five to three, namely Readiness, Opportunity Realisation and Uncertainty. - The Contact Centre employees were somewhat ready for change. - The Contact Centres needed to focus on all amended ADKAR factors in order to improve the readiness of the department. - The readiness levels in response to the roll out were more or less uniform. The study shows that given Eskom’s preference for the ADKAR model, future research within Eskom should therefore be conducted more circumspectly with respect to ascertaining the validity of the ADKAR factors. The study also makes mention that future work and/or research will need to be conducted, specifically on the readiness of the organisation itself, in order to improve the probability of transformational success. The ADKAR assessment is a people focused assessment and therefore focuses only on the readiness of the individual. Factors such as the adequacy of the current state of resources within the organisation, which incorporate aspects such as infrastructure, technology and staffing, will also need to be assessed to make a more holistic statement of change readiness. A concise review of the literature is incorporated into the Evaluation Report of Section 1 to underpin the study. In Section 2 a more extensive review of the literature is presented. Similarly, the design of the research is discussed in more detail in Section 3 to both describe and justify the appropriateness of the research methodology, and to give a detailed account of the way in which the research was carried out.
- Full Text:
- Date Issued: 2013
- Authors: Akoh, Emmanuel Inalegwu
- Date: 2013
- Subjects: Gbaran-Ubie oil and gas project -- Public opinion Community development -- Nigeria Economic development -- Nigeria -- Environmental aspects
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:715 , http://hdl.handle.net/10962/d1001869
- Description: This research paper is broken up into three sections, namely an evaluation report, a literature review and research methodology. The evaluation report section is the assessment of the current state of change readiness within the Eskom Contact Centre’s based upon what the literature on change readiness prescribes. The timing of the change readiness assessment is just prior to significant organisational change. The organisational change that was about to be initiated by the organisation was initiated from the boardroom of the most senior echelons of the organisation, and was directed in a top down approach, being a strategic organisational change. The change is deemed to be critical to the organisation being able to meet its long term strategic and sustainability objectives. A critical examination of the literature explored the meaning of change readiness, the importance of it and explained the consequences for organisations that commit to transformational agendas without being ready. Key concepts such as such resistance to change and organisational inertia are described and differentiated from change readiness. The ADKAR change model and its change readiness assessment instrument were used due to the organisations preference for the model. The ADKAR model formed the framework for the analyses of the data, the discussion of the results and the recommendations to the organisation. The research conducted was quantitative in nature; a questionnaire was distributed to the employees of the seven Eskom Contact centre sites around the country through an email. A slightly modified version of the ADKAR change readiness questionnaire was sent via email with an on-line questionnaire link on it; and questions on individual readiness for change were used to assess the level of readiness of the employees. Most of the descriptive and inferential statistics were analysed with the use of Excel (version, 2010), with Factor Analysis being done in Statistica. The results of the research showed that: [iii] - The factors as proposed by the ADKAR change readiness assessment questionnaire (i.e. Awareness, Desire, Knowledge, Ability and Reinforcement) are not different enough to be considered as independent factors for this data set. Based on factor analysis, the factors were subsequently amended from five to three, namely Readiness, Opportunity Realisation and Uncertainty. - The Contact Centre employees were somewhat ready for change. - The Contact Centres needed to focus on all amended ADKAR factors in order to improve the readiness of the department. - The readiness levels in response to the roll out were more or less uniform. The study shows that given Eskom’s preference for the ADKAR model, future research within Eskom should therefore be conducted more circumspectly with respect to ascertaining the validity of the ADKAR factors. The study also makes mention that future work and/or research will need to be conducted, specifically on the readiness of the organisation itself, in order to improve the probability of transformational success. The ADKAR assessment is a people focused assessment and therefore focuses only on the readiness of the individual. Factors such as the adequacy of the current state of resources within the organisation, which incorporate aspects such as infrastructure, technology and staffing, will also need to be assessed to make a more holistic statement of change readiness. A concise review of the literature is incorporated into the Evaluation Report of Section 1 to underpin the study. In Section 2 a more extensive review of the literature is presented. Similarly, the design of the research is discussed in more detail in Section 3 to both describe and justify the appropriateness of the research methodology, and to give a detailed account of the way in which the research was carried out.
- Full Text:
- Date Issued: 2013
The African coelacanth genome provides insights into tetrapod evolution:
- Amemiya, Chris T, Alföldi, Jessica, Lee, Alison P, Fan, Shaohua, Philippe, Herve´, MacCallum, Iain, Braasch, Ingo, Manousaki, Tereza, Schneider, Igor, Rohner, Nicolas, Organ, Chris, Chalopin, Domitille, Smith, Jeramiah J, Robinson, Mark, Dorrington, Rosemary A, Gerdol, Marco, Aken, Bronwen, Biscotti, Maria Assunta, Barucca, Marco, Baurain, Denis, Berlin, Aaron, Blatch, Gregory L, Buonocore, Francesco, Burmester, Thorsten, Campbell, Michael S, Canapa, Adriana, Cannon, John P, Christoffels, Alan, De Moro, Gianluca, Edkins, Adrienne L, Fan, Lin, Fausto, Anna Maria, Feiner, Nathalie, Forconi, Mariko, Gamieldien, Junaid, Gnerre, Sante, Gnirke, Andreas, Goldstone, Jared V, Haerty, Wilfried, Hahn, Mark E, Hesse, Uljana, Hoffmann, Steve, Johnson, Jeremy, Karchner, Sibel I, Kuraku, Shigehiro, Lara, Marcia, Levin, Joshua Z, Litman, Gary W, Mauceli, Evan, Miyake, Tsutomu, Mueller, M Gail, Nelson, David R, Nitsche, Anne, Olmo, Ettore, Ota, Tatsuya, Pallavicini, Alberto, Panji, Sumir, Picone, Barbara, Ponting, Chris P, Prohaska, Sonja J, Przybylski, Dariusz, Ratan Saha, Nil, Ravi, Vydianathan, Ribeiro, Filipe J, Sauka-Spengler, Tatjana, Scapigliati, Giuseppe, Searle, Stephen M J, Sharpe, Ted, Simakov, Oleg, Stadler, Peter F, Stegeman, John J, Sumiyama, Kenta, Tabbaa, Diana, Tafer, Hakim, Turner-Maier, Jason, van Heusden, Peter, White, Simon, Williams, Louise, Yandell, Mark, Brinkmann, Henner, Volff, Jean-Nicolas, Tabin, Clifford J, Shubin, Neil, Schartl, Manfred, Jaffe, David B, Postlethwait, John H, Venkatesh, Byrappa, Di Palma, Frederica, Lander, Eric S, Meyer, Axel, Lindblad-Toh, Kerstin
- Authors: Amemiya, Chris T , Alföldi, Jessica , Lee, Alison P , Fan, Shaohua , Philippe, Herve´ , MacCallum, Iain , Braasch, Ingo , Manousaki, Tereza , Schneider, Igor , Rohner, Nicolas , Organ, Chris , Chalopin, Domitille , Smith, Jeramiah J , Robinson, Mark , Dorrington, Rosemary A , Gerdol, Marco , Aken, Bronwen , Biscotti, Maria Assunta , Barucca, Marco , Baurain, Denis , Berlin, Aaron , Blatch, Gregory L , Buonocore, Francesco , Burmester, Thorsten , Campbell, Michael S , Canapa, Adriana , Cannon, John P , Christoffels, Alan , De Moro, Gianluca , Edkins, Adrienne L , Fan, Lin , Fausto, Anna Maria , Feiner, Nathalie , Forconi, Mariko , Gamieldien, Junaid , Gnerre, Sante , Gnirke, Andreas , Goldstone, Jared V , Haerty, Wilfried , Hahn, Mark E , Hesse, Uljana , Hoffmann, Steve , Johnson, Jeremy , Karchner, Sibel I , Kuraku, Shigehiro , Lara, Marcia , Levin, Joshua Z , Litman, Gary W , Mauceli, Evan , Miyake, Tsutomu , Mueller, M Gail , Nelson, David R , Nitsche, Anne , Olmo, Ettore , Ota, Tatsuya , Pallavicini, Alberto , Panji, Sumir , Picone, Barbara , Ponting, Chris P , Prohaska, Sonja J , Przybylski, Dariusz , Ratan Saha, Nil , Ravi, Vydianathan , Ribeiro, Filipe J , Sauka-Spengler, Tatjana , Scapigliati, Giuseppe , Searle, Stephen M J , Sharpe, Ted , Simakov, Oleg , Stadler, Peter F , Stegeman, John J , Sumiyama, Kenta , Tabbaa, Diana , Tafer, Hakim , Turner-Maier, Jason , van Heusden, Peter , White, Simon , Williams, Louise , Yandell, Mark , Brinkmann, Henner , Volff, Jean-Nicolas , Tabin, Clifford J , Shubin, Neil , Schartl, Manfred , Jaffe, David B , Postlethwait, John H , Venkatesh, Byrappa , Di Palma, Frederica , Lander, Eric S , Meyer, Axel , Lindblad-Toh, Kerstin
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/165030 , vital:41202 , DOI: 10.1038/nature12027
- Description: The discovery of a living coelacanth specimen in 1938 was remarkable, as this lineage of lobe-finned fish was thought to have become extinct 70 million years ago. The modern coelacanth looks remarkably similar to many of its ancient relatives, and its evolutionary proximity to our own fish ancestors provides a glimpse of the fish that first walked on land. Here we report the genome sequence of the African coelacanth, Latimeria chalumnae. Through a phylogenomic analysis, we conclude that the lungfish, and not the coelacanth, is the closest living relative of tetrapods. Coelacanth protein-coding genes are significantly more slowly evolving than those of tetrapods, unlike other genomic features. Analyses of changes in genes and regulatory elements during the vertebrate adaptation to land highlight genes involved in immunity, nitrogen excretion and the development of fins, tail, ear, eye, brain and olfaction. Functional assays of enhancers involved in the fin-to-limb transition and in the emergence of extra-embryonic tissues show the importance of the coelacanth genome as a blueprint for understanding tetrapod evolution.
- Full Text:
- Date Issued: 2013
- Authors: Amemiya, Chris T , Alföldi, Jessica , Lee, Alison P , Fan, Shaohua , Philippe, Herve´ , MacCallum, Iain , Braasch, Ingo , Manousaki, Tereza , Schneider, Igor , Rohner, Nicolas , Organ, Chris , Chalopin, Domitille , Smith, Jeramiah J , Robinson, Mark , Dorrington, Rosemary A , Gerdol, Marco , Aken, Bronwen , Biscotti, Maria Assunta , Barucca, Marco , Baurain, Denis , Berlin, Aaron , Blatch, Gregory L , Buonocore, Francesco , Burmester, Thorsten , Campbell, Michael S , Canapa, Adriana , Cannon, John P , Christoffels, Alan , De Moro, Gianluca , Edkins, Adrienne L , Fan, Lin , Fausto, Anna Maria , Feiner, Nathalie , Forconi, Mariko , Gamieldien, Junaid , Gnerre, Sante , Gnirke, Andreas , Goldstone, Jared V , Haerty, Wilfried , Hahn, Mark E , Hesse, Uljana , Hoffmann, Steve , Johnson, Jeremy , Karchner, Sibel I , Kuraku, Shigehiro , Lara, Marcia , Levin, Joshua Z , Litman, Gary W , Mauceli, Evan , Miyake, Tsutomu , Mueller, M Gail , Nelson, David R , Nitsche, Anne , Olmo, Ettore , Ota, Tatsuya , Pallavicini, Alberto , Panji, Sumir , Picone, Barbara , Ponting, Chris P , Prohaska, Sonja J , Przybylski, Dariusz , Ratan Saha, Nil , Ravi, Vydianathan , Ribeiro, Filipe J , Sauka-Spengler, Tatjana , Scapigliati, Giuseppe , Searle, Stephen M J , Sharpe, Ted , Simakov, Oleg , Stadler, Peter F , Stegeman, John J , Sumiyama, Kenta , Tabbaa, Diana , Tafer, Hakim , Turner-Maier, Jason , van Heusden, Peter , White, Simon , Williams, Louise , Yandell, Mark , Brinkmann, Henner , Volff, Jean-Nicolas , Tabin, Clifford J , Shubin, Neil , Schartl, Manfred , Jaffe, David B , Postlethwait, John H , Venkatesh, Byrappa , Di Palma, Frederica , Lander, Eric S , Meyer, Axel , Lindblad-Toh, Kerstin
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/165030 , vital:41202 , DOI: 10.1038/nature12027
- Description: The discovery of a living coelacanth specimen in 1938 was remarkable, as this lineage of lobe-finned fish was thought to have become extinct 70 million years ago. The modern coelacanth looks remarkably similar to many of its ancient relatives, and its evolutionary proximity to our own fish ancestors provides a glimpse of the fish that first walked on land. Here we report the genome sequence of the African coelacanth, Latimeria chalumnae. Through a phylogenomic analysis, we conclude that the lungfish, and not the coelacanth, is the closest living relative of tetrapods. Coelacanth protein-coding genes are significantly more slowly evolving than those of tetrapods, unlike other genomic features. Analyses of changes in genes and regulatory elements during the vertebrate adaptation to land highlight genes involved in immunity, nitrogen excretion and the development of fins, tail, ear, eye, brain and olfaction. Functional assays of enhancers involved in the fin-to-limb transition and in the emergence of extra-embryonic tissues show the importance of the coelacanth genome as a blueprint for understanding tetrapod evolution.
- Full Text:
- Date Issued: 2013
The Mandela Bay Development Agency's role in promoting community participation in the Helenvale Urban Renewal Project, Port Elizabeth
- Authors: Andrews, Christopher Lee
- Date: 2013
- Subjects: Community development -- South Africa -- Citizen participation , City planning -- South Africa -- Citizen participation , Urban renewal -- South Africa -- Eastern Cape , Economic development -- Citizen participation , South Africa -- Social policy -- Citizen participation
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:8315 , http://hdl.handle.net/10948/d1020095
- Description: Community participation in urban renewal projects has become important in the South African government’s efforts to address past imbalances and improving the livelihoods of socially excluded and marginalised communities. In order for the Helenvale Urban Renewal Project to be successful and bring about sustainable change, it is vital that the community be allowed and encouraged to play an active role in consultation and participation initiatives. This study outlines the importance of community participation, the types, the incentives and disincentives as well as the possible barriers to effective community participation. Findings from the analysis of the collected data indicates that a community project can only be successful if the implementing agent employs democratic principles whereby all residents are given a voice and are allowed to participate in the decision-making and implementation process. This study explores the concept of community participation in the Helenvale Urban Renewal Projects with particular reference to the role played by the Mandela Bay Development Agency in promoting community participation in the Helenvale Urban Renewal Projects (HURP), in Port Elizabeth.
- Full Text:
- Date Issued: 2013
- Authors: Andrews, Christopher Lee
- Date: 2013
- Subjects: Community development -- South Africa -- Citizen participation , City planning -- South Africa -- Citizen participation , Urban renewal -- South Africa -- Eastern Cape , Economic development -- Citizen participation , South Africa -- Social policy -- Citizen participation
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:8315 , http://hdl.handle.net/10948/d1020095
- Description: Community participation in urban renewal projects has become important in the South African government’s efforts to address past imbalances and improving the livelihoods of socially excluded and marginalised communities. In order for the Helenvale Urban Renewal Project to be successful and bring about sustainable change, it is vital that the community be allowed and encouraged to play an active role in consultation and participation initiatives. This study outlines the importance of community participation, the types, the incentives and disincentives as well as the possible barriers to effective community participation. Findings from the analysis of the collected data indicates that a community project can only be successful if the implementing agent employs democratic principles whereby all residents are given a voice and are allowed to participate in the decision-making and implementation process. This study explores the concept of community participation in the Helenvale Urban Renewal Projects with particular reference to the role played by the Mandela Bay Development Agency in promoting community participation in the Helenvale Urban Renewal Projects (HURP), in Port Elizabeth.
- Full Text:
- Date Issued: 2013
An investigation into the potential for implementing lean Grindrod Container Depot in Stanbridge Road
- Authors: Andrews, Ferderico Recardo
- Date: 2013
- Subjects: Lean manufacturing -- South Africa -- Port Elizabeth -- Management , Container industry -- South Africa -- Port Elizabeth , Just-in-time systems -- South Africa -- Port Elizabeth
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:8781 , http://hdl.handle.net/10948/d1012967
- Description: On a daily basis organizations strive to achieve maximum output with minimum input for greater levels of efficiency and profitability. However, in our dynamic modern world and economy the variables have changed dramatically and the time when business was simply about the ‘bottom line’ and all else was subordinate to the objective of profit, has come and gone. In the contemporary world of business today the variables are much broader and more dynamic, as people are no longer just employees, and suppliers are no longer people whose payment should be delayed for as long as possible. In addition, the environment is no longer to be plundered at all costs. Today Lean as an all-encompassing management philosophy with its emphasis on adding value, is more relevant in business than at any other point in business history. At a time when the global economy is facing some of its greatest challenges, it is ultimately business and its networks that, through effective management, can restore the global economy and provide livelihoods to millions of people. The Lean management philosophy is about constantly searching for the best possible way of doing things and eliminating waste in pursuit of value. If Lean is in pursuit of eliminating waste and turning it into value, and the customer, irrespective of required product, is in constant pursuit of finding value, then Lean is surely the management philosophy that can deliver the greatest level of customer satisfaction. This means satisfied customers returning to an organization for repeat sales as long as they perceive that the value they derive meets their expectations.
- Full Text:
- Date Issued: 2013
- Authors: Andrews, Ferderico Recardo
- Date: 2013
- Subjects: Lean manufacturing -- South Africa -- Port Elizabeth -- Management , Container industry -- South Africa -- Port Elizabeth , Just-in-time systems -- South Africa -- Port Elizabeth
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:8781 , http://hdl.handle.net/10948/d1012967
- Description: On a daily basis organizations strive to achieve maximum output with minimum input for greater levels of efficiency and profitability. However, in our dynamic modern world and economy the variables have changed dramatically and the time when business was simply about the ‘bottom line’ and all else was subordinate to the objective of profit, has come and gone. In the contemporary world of business today the variables are much broader and more dynamic, as people are no longer just employees, and suppliers are no longer people whose payment should be delayed for as long as possible. In addition, the environment is no longer to be plundered at all costs. Today Lean as an all-encompassing management philosophy with its emphasis on adding value, is more relevant in business than at any other point in business history. At a time when the global economy is facing some of its greatest challenges, it is ultimately business and its networks that, through effective management, can restore the global economy and provide livelihoods to millions of people. The Lean management philosophy is about constantly searching for the best possible way of doing things and eliminating waste in pursuit of value. If Lean is in pursuit of eliminating waste and turning it into value, and the customer, irrespective of required product, is in constant pursuit of finding value, then Lean is surely the management philosophy that can deliver the greatest level of customer satisfaction. This means satisfied customers returning to an organization for repeat sales as long as they perceive that the value they derive meets their expectations.
- Full Text:
- Date Issued: 2013
The reforms in the ministry of immigration and registration of persons in Kenya
- Authors: Angaine, Emily Karwitha
- Date: 2013
- Subjects: Public administration -- Kenya , Civil service -- Kenya , Organizational change -- Kenya
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:9237 , http://hdl.handle.net/10948/d1021099
- Description: Public service delivery is a major challenge to governments in both the developing and developed worlds. Governments have used various strategies to enhance service delivery. These strategies have resulted in varying outcomes and levels of service delivery. In the Kenyan government, the strategies to enhance service delivery include: Results Based Management strategies, service charters, Information Communication Technology (ICT), performance contracting, and strategic plans. This study is an investigation of reforms in the Ministry of Immigration and Registration of Persons (MIRP), Kenya. The study sought to investigate the effects of Results Based Management on service delivery; to investigate the effect of strategic planning on service delivery in Kenya; to investigate the effect of ICT on service delivery in Kenya; and to investigate the effect of Business Process Re-engineering (BPR) in service delivery. This study utilized a descriptive research design where the researcher investigated and collected factual data to describe the situation or problem. Secondary data was sourced from document reviews. Data was analyzed as it was collected. The researcher classified the data in terms of the topic, themes and content. The broad topic and themes of the data collected was based on the research objectives of this study and included: use of Information Communication Technology, Business Process Re-engineering, Results Based Management, and strategic planning. The findings revealed that the department has utilized Results Based Management, strategic planning, Information Communication Technology, as well as Business Process Re-engineering tools in reforming public service delivery towards effectiveness and efficiency. Furthermore, this study also revealed that the department has accrued various benefits as a consequence of these tools despite the challenges facing their implementation. According to the report, the reform programs have enhanced greater transparency in service delivery, improved accountability, responsibility in employees, enhanced policy coherence and strengthened capacity. these advantages are evident to the Department of Immigration. the study recommends that the use of Results Based Management, ICT and Business Process Re-engineering policies and changes should be implemented to improve service delivery in the Ministry of Immigration and Registration of Persons. In addition, the study recommends the following further studies: the study should be replicated to other public organizations (agencies) to allow for comparison of the findings of this study; the study recommends research on the success of public organizations in achieving objectives of their strategic planning (this is based on the challenges identified facing strategic planning in the Department of Immigration); and, finally, this study recommends future research on the relationship between Business Process Re-engineering (BPR) and efficient service delivery. This study will provide insight to what extent BPR affects service delivery.
- Full Text:
- Date Issued: 2013
- Authors: Angaine, Emily Karwitha
- Date: 2013
- Subjects: Public administration -- Kenya , Civil service -- Kenya , Organizational change -- Kenya
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:9237 , http://hdl.handle.net/10948/d1021099
- Description: Public service delivery is a major challenge to governments in both the developing and developed worlds. Governments have used various strategies to enhance service delivery. These strategies have resulted in varying outcomes and levels of service delivery. In the Kenyan government, the strategies to enhance service delivery include: Results Based Management strategies, service charters, Information Communication Technology (ICT), performance contracting, and strategic plans. This study is an investigation of reforms in the Ministry of Immigration and Registration of Persons (MIRP), Kenya. The study sought to investigate the effects of Results Based Management on service delivery; to investigate the effect of strategic planning on service delivery in Kenya; to investigate the effect of ICT on service delivery in Kenya; and to investigate the effect of Business Process Re-engineering (BPR) in service delivery. This study utilized a descriptive research design where the researcher investigated and collected factual data to describe the situation or problem. Secondary data was sourced from document reviews. Data was analyzed as it was collected. The researcher classified the data in terms of the topic, themes and content. The broad topic and themes of the data collected was based on the research objectives of this study and included: use of Information Communication Technology, Business Process Re-engineering, Results Based Management, and strategic planning. The findings revealed that the department has utilized Results Based Management, strategic planning, Information Communication Technology, as well as Business Process Re-engineering tools in reforming public service delivery towards effectiveness and efficiency. Furthermore, this study also revealed that the department has accrued various benefits as a consequence of these tools despite the challenges facing their implementation. According to the report, the reform programs have enhanced greater transparency in service delivery, improved accountability, responsibility in employees, enhanced policy coherence and strengthened capacity. these advantages are evident to the Department of Immigration. the study recommends that the use of Results Based Management, ICT and Business Process Re-engineering policies and changes should be implemented to improve service delivery in the Ministry of Immigration and Registration of Persons. In addition, the study recommends the following further studies: the study should be replicated to other public organizations (agencies) to allow for comparison of the findings of this study; the study recommends research on the success of public organizations in achieving objectives of their strategic planning (this is based on the challenges identified facing strategic planning in the Department of Immigration); and, finally, this study recommends future research on the relationship between Business Process Re-engineering (BPR) and efficient service delivery. This study will provide insight to what extent BPR affects service delivery.
- Full Text:
- Date Issued: 2013