Axial coordination of zinc and silicon phthalocyanines to silver and gold nanoparticles: an investigation of their photophysicochemical and antimicrobial behavior
- Masilela, Nkosiphile, Antunes, Edith M, Nyokong, Tebello
- Authors: Masilela, Nkosiphile , Antunes, Edith M , Nyokong, Tebello
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/241818 , vital:50972 , xlink:href="https://doi.org/10.1142/S1088424613500016"
- Description: This work reports on the axial coordination of zinc phthalocyanine and bis-(1,6-hexanedithiol) silicon phthalocyanine to silver and gold nanoparticles. Red shifting of absorption spectra of the phthalocyanine complexes was observed after conjugation with the nanoparticles. An improvement in the photophysicochemical behavior and antimicrobial activity was achieved in the presence of metal nanoparticles for both complexes. A decrease in triplet lifetimes was observed for all the phthalocyanine metal nanoparticle conjugates. The Zn phthalocyanine complex gave the highest triplet and singlet oxygen quantum yield in the presence of gold nanoparticles. On the other hand, the bacterial inhibition was found to be best for the Si phthalocyanine derivative in the presence of nanoparticles compared to the Zn phthalocyanine counterpart. The highest antimicrobial activity was achieved for both conjugates against B. subtilis compared to S. aureaus both in the dark and under illumination with light.
- Full Text:
- Authors: Masilela, Nkosiphile , Antunes, Edith M , Nyokong, Tebello
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/241818 , vital:50972 , xlink:href="https://doi.org/10.1142/S1088424613500016"
- Description: This work reports on the axial coordination of zinc phthalocyanine and bis-(1,6-hexanedithiol) silicon phthalocyanine to silver and gold nanoparticles. Red shifting of absorption spectra of the phthalocyanine complexes was observed after conjugation with the nanoparticles. An improvement in the photophysicochemical behavior and antimicrobial activity was achieved in the presence of metal nanoparticles for both complexes. A decrease in triplet lifetimes was observed for all the phthalocyanine metal nanoparticle conjugates. The Zn phthalocyanine complex gave the highest triplet and singlet oxygen quantum yield in the presence of gold nanoparticles. On the other hand, the bacterial inhibition was found to be best for the Si phthalocyanine derivative in the presence of nanoparticles compared to the Zn phthalocyanine counterpart. The highest antimicrobial activity was achieved for both conjugates against B. subtilis compared to S. aureaus both in the dark and under illumination with light.
- Full Text:
Barriers to public participation in developmental projects : a Nigerian community perspective (case study of the Gbaran-Ubie oil and gas project)
- Authors: Akoh, Emmanuel Inalegwu
- Date: 2013
- Subjects: Gbaran-Ubie oil and gas project -- Public opinion Community development -- Nigeria Economic development -- Nigeria -- Environmental aspects
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:715 , http://hdl.handle.net/10962/d1001869
- Description: This research paper is broken up into three sections, namely an evaluation report, a literature review and research methodology. The evaluation report section is the assessment of the current state of change readiness within the Eskom Contact Centre’s based upon what the literature on change readiness prescribes. The timing of the change readiness assessment is just prior to significant organisational change. The organisational change that was about to be initiated by the organisation was initiated from the boardroom of the most senior echelons of the organisation, and was directed in a top down approach, being a strategic organisational change. The change is deemed to be critical to the organisation being able to meet its long term strategic and sustainability objectives. A critical examination of the literature explored the meaning of change readiness, the importance of it and explained the consequences for organisations that commit to transformational agendas without being ready. Key concepts such as such resistance to change and organisational inertia are described and differentiated from change readiness. The ADKAR change model and its change readiness assessment instrument were used due to the organisations preference for the model. The ADKAR model formed the framework for the analyses of the data, the discussion of the results and the recommendations to the organisation. The research conducted was quantitative in nature; a questionnaire was distributed to the employees of the seven Eskom Contact centre sites around the country through an email. A slightly modified version of the ADKAR change readiness questionnaire was sent via email with an on-line questionnaire link on it; and questions on individual readiness for change were used to assess the level of readiness of the employees. Most of the descriptive and inferential statistics were analysed with the use of Excel (version, 2010), with Factor Analysis being done in Statistica. The results of the research showed that: [iii] - The factors as proposed by the ADKAR change readiness assessment questionnaire (i.e. Awareness, Desire, Knowledge, Ability and Reinforcement) are not different enough to be considered as independent factors for this data set. Based on factor analysis, the factors were subsequently amended from five to three, namely Readiness, Opportunity Realisation and Uncertainty. - The Contact Centre employees were somewhat ready for change. - The Contact Centres needed to focus on all amended ADKAR factors in order to improve the readiness of the department. - The readiness levels in response to the roll out were more or less uniform. The study shows that given Eskom’s preference for the ADKAR model, future research within Eskom should therefore be conducted more circumspectly with respect to ascertaining the validity of the ADKAR factors. The study also makes mention that future work and/or research will need to be conducted, specifically on the readiness of the organisation itself, in order to improve the probability of transformational success. The ADKAR assessment is a people focused assessment and therefore focuses only on the readiness of the individual. Factors such as the adequacy of the current state of resources within the organisation, which incorporate aspects such as infrastructure, technology and staffing, will also need to be assessed to make a more holistic statement of change readiness. A concise review of the literature is incorporated into the Evaluation Report of Section 1 to underpin the study. In Section 2 a more extensive review of the literature is presented. Similarly, the design of the research is discussed in more detail in Section 3 to both describe and justify the appropriateness of the research methodology, and to give a detailed account of the way in which the research was carried out.
- Full Text:
- Authors: Akoh, Emmanuel Inalegwu
- Date: 2013
- Subjects: Gbaran-Ubie oil and gas project -- Public opinion Community development -- Nigeria Economic development -- Nigeria -- Environmental aspects
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:715 , http://hdl.handle.net/10962/d1001869
- Description: This research paper is broken up into three sections, namely an evaluation report, a literature review and research methodology. The evaluation report section is the assessment of the current state of change readiness within the Eskom Contact Centre’s based upon what the literature on change readiness prescribes. The timing of the change readiness assessment is just prior to significant organisational change. The organisational change that was about to be initiated by the organisation was initiated from the boardroom of the most senior echelons of the organisation, and was directed in a top down approach, being a strategic organisational change. The change is deemed to be critical to the organisation being able to meet its long term strategic and sustainability objectives. A critical examination of the literature explored the meaning of change readiness, the importance of it and explained the consequences for organisations that commit to transformational agendas without being ready. Key concepts such as such resistance to change and organisational inertia are described and differentiated from change readiness. The ADKAR change model and its change readiness assessment instrument were used due to the organisations preference for the model. The ADKAR model formed the framework for the analyses of the data, the discussion of the results and the recommendations to the organisation. The research conducted was quantitative in nature; a questionnaire was distributed to the employees of the seven Eskom Contact centre sites around the country through an email. A slightly modified version of the ADKAR change readiness questionnaire was sent via email with an on-line questionnaire link on it; and questions on individual readiness for change were used to assess the level of readiness of the employees. Most of the descriptive and inferential statistics were analysed with the use of Excel (version, 2010), with Factor Analysis being done in Statistica. The results of the research showed that: [iii] - The factors as proposed by the ADKAR change readiness assessment questionnaire (i.e. Awareness, Desire, Knowledge, Ability and Reinforcement) are not different enough to be considered as independent factors for this data set. Based on factor analysis, the factors were subsequently amended from five to three, namely Readiness, Opportunity Realisation and Uncertainty. - The Contact Centre employees were somewhat ready for change. - The Contact Centres needed to focus on all amended ADKAR factors in order to improve the readiness of the department. - The readiness levels in response to the roll out were more or less uniform. The study shows that given Eskom’s preference for the ADKAR model, future research within Eskom should therefore be conducted more circumspectly with respect to ascertaining the validity of the ADKAR factors. The study also makes mention that future work and/or research will need to be conducted, specifically on the readiness of the organisation itself, in order to improve the probability of transformational success. The ADKAR assessment is a people focused assessment and therefore focuses only on the readiness of the individual. Factors such as the adequacy of the current state of resources within the organisation, which incorporate aspects such as infrastructure, technology and staffing, will also need to be assessed to make a more holistic statement of change readiness. A concise review of the literature is incorporated into the Evaluation Report of Section 1 to underpin the study. In Section 2 a more extensive review of the literature is presented. Similarly, the design of the research is discussed in more detail in Section 3 to both describe and justify the appropriateness of the research methodology, and to give a detailed account of the way in which the research was carried out.
- Full Text:
Behavioural observations of the common octopus Octopus vulgaris in Baía dos Tigres, southern Angola
- de Beer, Chénelle L, Potts, Warren M
- Authors: de Beer, Chénelle L , Potts, Warren M
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/123233 , vital:35419 , https://doi.org/10.2989/1814232X.2013.847496
- Description: The common octopus Octopus vulgaris (Cuvier 1797) is a shallow-water cephalopod species that inhabits coastal rocky areas or reefs. It is the most studied and widely distributed species in its genus. This species has a short lifespan and a rapid growth rate of over 5% of body weight per day (García and Valverde 2006, Ibáñez and Keyl 2010). It is semelparous, with females laying large strings of eggs that they attach to the substrata in their dens (Hernández- García et al. 2002). There is a paucity of information on the preferred habitat and behaviour of O. vulgaris in its natural environment (Anderson 1997, Meisel et al. 2006). The species has been classified as highly mobile, yet resident individuals may exhibit high levels of territoriality. Its mobility allows it to move efficiently between a wide range of suitable habitats (Katsanevakis and Verriopoulos 2004). Octopus make use of both tactile and visual senses to forage, feed and seek suitable shelter (Forsythe and Hanlon 1997, Carvalho and Sousa Reis 2003, Rodríguez-Rúa et al. 2005). They are active feeders that seek out prey such as crustaceans, sessile molluscs and small fish (Forsythe and Hanlon 1997, Boyle and Rodhouse 2005), as well as their smaller conspecifics (Ibáñez and Keyl 2010). The majority of behavioural ecology studies on octopus have been on captive individuals. However, the activity patterns (Mather 1988), foraging strategies and aspects of the movement behaviour (Mather and O’Dor 1991) of juvenile O. vulgaris in the wild were described for an exploited population in Bermuda. Because intertidal octopus stocks are exploited by recreational, subsistence and artisanal fisheries (e.g. Oosthuizen and Smale 2003, Sauer et al. 2011), there are few opportunities to study the behavioural ecology of unexploited populations. An opportunity arose to study various behavioural patterns in an unexploited population in Baía dos Tigres, a large (~200 km2) coastal embayment situated on an isolated stretch of the southern Angolan coast (Figure 1). This study provides information on the population size structure, aspects of the movement behaviour, activity patterns and foraging strategies of O. vulgaris in this largely unstudied coastal embayment.
- Full Text:
- Authors: de Beer, Chénelle L , Potts, Warren M
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/123233 , vital:35419 , https://doi.org/10.2989/1814232X.2013.847496
- Description: The common octopus Octopus vulgaris (Cuvier 1797) is a shallow-water cephalopod species that inhabits coastal rocky areas or reefs. It is the most studied and widely distributed species in its genus. This species has a short lifespan and a rapid growth rate of over 5% of body weight per day (García and Valverde 2006, Ibáñez and Keyl 2010). It is semelparous, with females laying large strings of eggs that they attach to the substrata in their dens (Hernández- García et al. 2002). There is a paucity of information on the preferred habitat and behaviour of O. vulgaris in its natural environment (Anderson 1997, Meisel et al. 2006). The species has been classified as highly mobile, yet resident individuals may exhibit high levels of territoriality. Its mobility allows it to move efficiently between a wide range of suitable habitats (Katsanevakis and Verriopoulos 2004). Octopus make use of both tactile and visual senses to forage, feed and seek suitable shelter (Forsythe and Hanlon 1997, Carvalho and Sousa Reis 2003, Rodríguez-Rúa et al. 2005). They are active feeders that seek out prey such as crustaceans, sessile molluscs and small fish (Forsythe and Hanlon 1997, Boyle and Rodhouse 2005), as well as their smaller conspecifics (Ibáñez and Keyl 2010). The majority of behavioural ecology studies on octopus have been on captive individuals. However, the activity patterns (Mather 1988), foraging strategies and aspects of the movement behaviour (Mather and O’Dor 1991) of juvenile O. vulgaris in the wild were described for an exploited population in Bermuda. Because intertidal octopus stocks are exploited by recreational, subsistence and artisanal fisheries (e.g. Oosthuizen and Smale 2003, Sauer et al. 2011), there are few opportunities to study the behavioural ecology of unexploited populations. An opportunity arose to study various behavioural patterns in an unexploited population in Baía dos Tigres, a large (~200 km2) coastal embayment situated on an isolated stretch of the southern Angolan coast (Figure 1). This study provides information on the population size structure, aspects of the movement behaviour, activity patterns and foraging strategies of O. vulgaris in this largely unstudied coastal embayment.
- Full Text:
Being South African and belonging: the status and practice of mediated citizenship in a new democracy
- Wasserman, Herman, Garman, Anthea
- Authors: Wasserman, Herman , Garman, Anthea
- Date: 2013
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/159769 , vital:40342 , ISBN 978-1-84888-186-0
- Description: Democratic South Africa, with its highly inclusive constitution and embrace of all races, creeds and colours, could be understood as having an ideal form of citizenship to be emulated by other nations. At the heart of the 1996 constitution is the eradication of apartheid separation and the provision that all South Africans have shared humanity (‘ubuntu’). The Truth and Reconciliation Commission entrenched three founding critical ideas in public life: the right to talk, the recognition of shared humanity and the impulse to speak out about the horrors of the past. As a result the public sphere is filled with a great outpouring of personal stories and experiences in both the mainstream and popular forms of media. But South Africans continue to be preoccupied with the status of their citizenship; who a South African is and who belongs is uppermost in many public conversations.
- Full Text:
- Authors: Wasserman, Herman , Garman, Anthea
- Date: 2013
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/159769 , vital:40342 , ISBN 978-1-84888-186-0
- Description: Democratic South Africa, with its highly inclusive constitution and embrace of all races, creeds and colours, could be understood as having an ideal form of citizenship to be emulated by other nations. At the heart of the 1996 constitution is the eradication of apartheid separation and the provision that all South Africans have shared humanity (‘ubuntu’). The Truth and Reconciliation Commission entrenched three founding critical ideas in public life: the right to talk, the recognition of shared humanity and the impulse to speak out about the horrors of the past. As a result the public sphere is filled with a great outpouring of personal stories and experiences in both the mainstream and popular forms of media. But South Africans continue to be preoccupied with the status of their citizenship; who a South African is and who belongs is uppermost in many public conversations.
- Full Text:
Beyond the threshold: explorations of liminality in literature. A book review
- Authors: Dass, Minesh
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/142520 , vital:38087 , DOI: 10.1080/10131752.2013.783395
- Description: Beyond the Threshold: Explorations of Liminality in Literature, an edited collection of essays, is the culmination of research project on liminality done under the title “Poetics of boundaries and Hybridity”. This venture was undertaken in what is now known as the Research Unit Languages and Literature in the South African Context at North-West University. Some of the articles were initially presented at a conference on “Hybridity, Liminality and Boundaries” which was held in Potchefstroom from 30 June to 2 July 2005. Furthermore, shorter versions of some chapters have already been published in a special edition of Literator (Vol. 27. 1).
- Full Text:
- Authors: Dass, Minesh
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/142520 , vital:38087 , DOI: 10.1080/10131752.2013.783395
- Description: Beyond the Threshold: Explorations of Liminality in Literature, an edited collection of essays, is the culmination of research project on liminality done under the title “Poetics of boundaries and Hybridity”. This venture was undertaken in what is now known as the Research Unit Languages and Literature in the South African Context at North-West University. Some of the articles were initially presented at a conference on “Hybridity, Liminality and Boundaries” which was held in Potchefstroom from 30 June to 2 July 2005. Furthermore, shorter versions of some chapters have already been published in a special edition of Literator (Vol. 27. 1).
- Full Text:
Biochemical characterisation of Pfj2, a Plasmodium falciparum heat shock protein 40 chaperone potentially involved in protein quality control in the endoplasmic reticulum
- Authors: Afolayan, Omolola Folasade
- Date: 2013
- Subjects: Plasmodium falciparum Endoplasmic reticulum Heat shock proteins Malaria , Mosquito-borne infectious disease
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3883 , http://hdl.handle.net/10962/d1001617
- Description: Plasmodium falciparum is a protozoan parasite that causes a severe form of malaria, a mosquito-borne infectious disease in humans. P. falciparum encodes a number of proteins to facilitate its life-cycle, including a type II heat shock protein 40 (Hsp40), Pfj2. Pfj2 shows a degree of homology to human ERdj5, a resident protein of the endoplasmic reticulum (ER) that promotes protein quality control by facilitating the degradation of misfolded proteins. The overall aim of this study was to further understand the function of Pfj2 in the P. falciparum cell by characterising it biochemically. A bioinformatic analysis of Pfj2 was carried out to enable the identification of a potential ER signal sequence and cleavage site. Furthermore, an analysis of Pfj2 protein sequence was performed to compare domain similarities and identities with typical type II Hsp40s namely, human ERdj5, S. cerevisiae Sis1, human Hsj1a and human DnaJB4. The method used included the insertion of the codon-optimised coding sequence for the processed ER form of Pfj2 into the prokaryotic expression vector, pQE30, to enable overproduction of a histidine-tagged protein. A 62 kDa His₆-Pfj2 was successfully expressed in Escherichia coli and purified using denaturing nickel affinity chromatography. ATPase assays were performed to determine the ability of His₆- Pfj2 to stimulate the chaperone activity of the ER Hsp70, also called immunoglobulin binding protein (BiP). Initial studies were conducted on readily available mammalian His₆-BiP as a control, which was shown to have an intrinsic activity of 12.07±3.92 nmolPi/min/mg. His₆- Pfj2 did not stimulate the ATPase activity of mammalian His₆-BiP, suggesting that it either could not act as a co-chaperone of mammalian His₆-BiP (specificity), or it required a misfolded substrate in the system. Therefore, ongoing studies are addressing the interaction of Pfj2 and misfolded substrates with P. falciparum BiP. The results of these studies will further our understanding of a poorly-studied parasite chaperone that represents a potential drug target for development of novel strategies for the control of a serious human disease
- Full Text:
- Authors: Afolayan, Omolola Folasade
- Date: 2013
- Subjects: Plasmodium falciparum Endoplasmic reticulum Heat shock proteins Malaria , Mosquito-borne infectious disease
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:3883 , http://hdl.handle.net/10962/d1001617
- Description: Plasmodium falciparum is a protozoan parasite that causes a severe form of malaria, a mosquito-borne infectious disease in humans. P. falciparum encodes a number of proteins to facilitate its life-cycle, including a type II heat shock protein 40 (Hsp40), Pfj2. Pfj2 shows a degree of homology to human ERdj5, a resident protein of the endoplasmic reticulum (ER) that promotes protein quality control by facilitating the degradation of misfolded proteins. The overall aim of this study was to further understand the function of Pfj2 in the P. falciparum cell by characterising it biochemically. A bioinformatic analysis of Pfj2 was carried out to enable the identification of a potential ER signal sequence and cleavage site. Furthermore, an analysis of Pfj2 protein sequence was performed to compare domain similarities and identities with typical type II Hsp40s namely, human ERdj5, S. cerevisiae Sis1, human Hsj1a and human DnaJB4. The method used included the insertion of the codon-optimised coding sequence for the processed ER form of Pfj2 into the prokaryotic expression vector, pQE30, to enable overproduction of a histidine-tagged protein. A 62 kDa His₆-Pfj2 was successfully expressed in Escherichia coli and purified using denaturing nickel affinity chromatography. ATPase assays were performed to determine the ability of His₆- Pfj2 to stimulate the chaperone activity of the ER Hsp70, also called immunoglobulin binding protein (BiP). Initial studies were conducted on readily available mammalian His₆-BiP as a control, which was shown to have an intrinsic activity of 12.07±3.92 nmolPi/min/mg. His₆- Pfj2 did not stimulate the ATPase activity of mammalian His₆-BiP, suggesting that it either could not act as a co-chaperone of mammalian His₆-BiP (specificity), or it required a misfolded substrate in the system. Therefore, ongoing studies are addressing the interaction of Pfj2 and misfolded substrates with P. falciparum BiP. The results of these studies will further our understanding of a poorly-studied parasite chaperone that represents a potential drug target for development of novel strategies for the control of a serious human disease
- Full Text:
Biogeographical boundaries, functional group structure and diversity of rocky shore communities along the Argentinean coast
- Wieters, Evie A, McQuaid, Christopher D, Palomo, Gabriela, Pappalardo, Paula
- Authors: Wieters, Evie A , McQuaid, Christopher D , Palomo, Gabriela , Pappalardo, Paula
- Date: 2013
- Language: English
- Type: Article
- Identifier: vital:6835 , http://hdl.handle.net/10962/d1010956
- Description: We investigate the extent to which functional structure and spatial variability of intertidal communities coincide with major biogeographical boundaries, areas where extensive compositional changes in the biota are observed over a limited geographic extension. We then investigate whether spatial variation in the biomass of functional groups, over geographic (10′s km) and local (10′s m) scales, could be associated to species diversity within and among these groups. Functional community structure expressed as abundance (density, cover and biomass) and composition of major functional groups was quantified through field surveys at 20 rocky intertidal shores spanning six degrees of latitude along the southwest Atlantic coast of Argentina and extending across the boundaries between the Argentinean and Magellanic Provinces. Patterns of abundance of individual functional groups were not uniformly matched with biogeographical regions. Only ephemeral algae showed an abrupt geographical discontinuity coincident with changes in biogeographic boundaries, and this was limited to the mid intertidal zone. We identified 3–4 main ‘groups’ of sites in terms of the total and relative abundance of the major functional groups, but these did not coincide with biogeographical boundaries, nor did they follow latitudinal arrangement. Thus, processes that determine the functional structure of these intertidal communities are insensitive to biogeographical boundaries. Over both geographical and local spatial scales, and for most functional groups and tidal levels, increases in species richness within the functional group was significantly associated to increased total biomass and reduced spatial variability of the group. These results suggest that species belonging to the same functional group are sufficiently uncorrelated over space (i.e. metres and site-to-site ) to stabilize patterns of biomass variability and, in this manner, provide a buffer, or “insurance”, against spatial variability in environmental conditions.
- Full Text:
- Authors: Wieters, Evie A , McQuaid, Christopher D , Palomo, Gabriela , Pappalardo, Paula
- Date: 2013
- Language: English
- Type: Article
- Identifier: vital:6835 , http://hdl.handle.net/10962/d1010956
- Description: We investigate the extent to which functional structure and spatial variability of intertidal communities coincide with major biogeographical boundaries, areas where extensive compositional changes in the biota are observed over a limited geographic extension. We then investigate whether spatial variation in the biomass of functional groups, over geographic (10′s km) and local (10′s m) scales, could be associated to species diversity within and among these groups. Functional community structure expressed as abundance (density, cover and biomass) and composition of major functional groups was quantified through field surveys at 20 rocky intertidal shores spanning six degrees of latitude along the southwest Atlantic coast of Argentina and extending across the boundaries between the Argentinean and Magellanic Provinces. Patterns of abundance of individual functional groups were not uniformly matched with biogeographical regions. Only ephemeral algae showed an abrupt geographical discontinuity coincident with changes in biogeographic boundaries, and this was limited to the mid intertidal zone. We identified 3–4 main ‘groups’ of sites in terms of the total and relative abundance of the major functional groups, but these did not coincide with biogeographical boundaries, nor did they follow latitudinal arrangement. Thus, processes that determine the functional structure of these intertidal communities are insensitive to biogeographical boundaries. Over both geographical and local spatial scales, and for most functional groups and tidal levels, increases in species richness within the functional group was significantly associated to increased total biomass and reduced spatial variability of the group. These results suggest that species belonging to the same functional group are sufficiently uncorrelated over space (i.e. metres and site-to-site ) to stabilize patterns of biomass variability and, in this manner, provide a buffer, or “insurance”, against spatial variability in environmental conditions.
- Full Text:
Biology and ecology of largemouth bass Micropterus salmoides in two temperate South African impoundments
- Authors: Taylor, Geraldine Claire
- Date: 2013 , 2013-03-19
- Subjects: Largemouth bass -- Ecology -- South Africa -- Eastern Cape , Largemouth bass -- Behavior -- South Africa -- Eastern Cape , Largemouth bass -- Growth -- South Africa -- Eastern Cape , Largemouth bass -- Reproduction -- South Africa -- Eastern Cape , Largemouth bass -- Food -- South Africa -- Eastern Cape , Largemouth bass -- Age determination -- South Africa -- Eastern Cape , Introduced fishes -- South Africa -- Eastern Cape , Fish populations -- South Africa -- Eastern Cape , Otoliths , Largemouth bass -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5181 , http://hdl.handle.net/10962/d1001668 , Largemouth bass -- Ecology -- South Africa -- Eastern Cape , Largemouth bass -- Behavior -- South Africa -- Eastern Cape , Largemouth bass -- Growth -- South Africa -- Eastern Cape , Largemouth bass -- Reproduction -- South Africa -- Eastern Cape , Largemouth bass -- Food -- South Africa -- Eastern Cape , Largemouth bass -- Age determination -- South Africa -- Eastern Cape , Introduced fishes -- South Africa -- Eastern Cape , Fish populations -- South Africa -- Eastern Cape , Otoliths
- Description: Globally largemouth bass Micropterus salmoides is one of the most widely introduced game fish species which has now become invasive in many countries. Well researched in its native North America, there are few studies on this species in its introduced range. This study aims to improve the understanding of the biology and ecology of M. salmoides in temperate southern Africa, where it threatens native biota and supports a popular recreational fishery. This aim was addressed by assessing the age, growth, maturity, reproductive seasonality, feeding, mortality and movement, of two M. salmoides populations from Wriggleswade (1000 ha, 723 m amsl, 32º35'S; 27º33'E) and Mankazana (35 ha, 66 m amsl, 33º09'S; 26º57'E) Dams in the Eastern Cape of South Africa. The most suitable structure for ageing M. salmoides was determined by comparing the accuracy and precision of otoliths and scales. Scales tended to underestimate the age of fish older than five years. Sectioned sagittal otoliths were the more precise structures for ageing (otoliths CV = 15.8 vs. scales CV = 21.9). The periodicity of growth zone formation was validated as annual for otoliths using both edge analysis (EA), and mark recapture of chemically tagged fish (MRCT). EA indicated one annual peak in the frequency of opaque margins between September and January using a periodic logistic regression and a binomial model linked with a von Mises distribution for circular data. MRCT showed the linear relationship between time at liberty and number of growth zones distal to the fluorescent band was not significantly different from one (slope = 0.89). Reproduction was similar to that in native and non-native populations with fish reaching age at 50% maturity at ca. two years, and length at 50% maturity at 259-290 mm FL depending on growth rate. Spawning season occurred in the spring months of August to October. The Wriggleswade Dam population reached similar ages (14 yrs) to populations in temperate North America. Growth was described using the von Bertalanffy growth equations of (Lt = 420(1-ᵉ⁻°·³³⁽ᵗ⁺°·²¹⁾) mm FL in Wriggleswade and (Lt = 641(1-ᵉ⁻°·²²⁽ᵗ⁺°·⁷⁶⁾) mm FL in Mankazana. Growth performance of both populations, described using the phi prime index, seemed to be correlated with temperature and the Wriggleswade Dam population (Φ’ = 2.8) grew similarly to those native temperate North American populations and the non-native populations of Japan and Italy. The Mankazana population had a higher growth performance (Φ’ = 2.9) compared to Wriggleswade and growth was comparable to other nonnative African populations. Both populations utilised all available food resources, with the Mankazana population being in better condition than the Wriggleswade population as a result of a more diverse diet, highly abundant food sources throughout the year, and potentially the adoption of a more conservative ambush feeding strategy. The Wriggleswade population, which was utilised by competitive anglers, showed low mortality rates (Z = 0.43 yr⁻¹), and translocated fish dispersed from the common release site, travelling an average distance of 4km over the study time of 494 days. These results indicated that M. salmoides are successful in these environments, growing at expected temperature dependant rates, maturing at two years, using all available food groups, and suffering from low mortality. It is therefore likely that they will persist in South Africa and establish where introduced.
- Full Text:
- Authors: Taylor, Geraldine Claire
- Date: 2013 , 2013-03-19
- Subjects: Largemouth bass -- Ecology -- South Africa -- Eastern Cape , Largemouth bass -- Behavior -- South Africa -- Eastern Cape , Largemouth bass -- Growth -- South Africa -- Eastern Cape , Largemouth bass -- Reproduction -- South Africa -- Eastern Cape , Largemouth bass -- Food -- South Africa -- Eastern Cape , Largemouth bass -- Age determination -- South Africa -- Eastern Cape , Introduced fishes -- South Africa -- Eastern Cape , Fish populations -- South Africa -- Eastern Cape , Otoliths , Largemouth bass -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5181 , http://hdl.handle.net/10962/d1001668 , Largemouth bass -- Ecology -- South Africa -- Eastern Cape , Largemouth bass -- Behavior -- South Africa -- Eastern Cape , Largemouth bass -- Growth -- South Africa -- Eastern Cape , Largemouth bass -- Reproduction -- South Africa -- Eastern Cape , Largemouth bass -- Food -- South Africa -- Eastern Cape , Largemouth bass -- Age determination -- South Africa -- Eastern Cape , Introduced fishes -- South Africa -- Eastern Cape , Fish populations -- South Africa -- Eastern Cape , Otoliths
- Description: Globally largemouth bass Micropterus salmoides is one of the most widely introduced game fish species which has now become invasive in many countries. Well researched in its native North America, there are few studies on this species in its introduced range. This study aims to improve the understanding of the biology and ecology of M. salmoides in temperate southern Africa, where it threatens native biota and supports a popular recreational fishery. This aim was addressed by assessing the age, growth, maturity, reproductive seasonality, feeding, mortality and movement, of two M. salmoides populations from Wriggleswade (1000 ha, 723 m amsl, 32º35'S; 27º33'E) and Mankazana (35 ha, 66 m amsl, 33º09'S; 26º57'E) Dams in the Eastern Cape of South Africa. The most suitable structure for ageing M. salmoides was determined by comparing the accuracy and precision of otoliths and scales. Scales tended to underestimate the age of fish older than five years. Sectioned sagittal otoliths were the more precise structures for ageing (otoliths CV = 15.8 vs. scales CV = 21.9). The periodicity of growth zone formation was validated as annual for otoliths using both edge analysis (EA), and mark recapture of chemically tagged fish (MRCT). EA indicated one annual peak in the frequency of opaque margins between September and January using a periodic logistic regression and a binomial model linked with a von Mises distribution for circular data. MRCT showed the linear relationship between time at liberty and number of growth zones distal to the fluorescent band was not significantly different from one (slope = 0.89). Reproduction was similar to that in native and non-native populations with fish reaching age at 50% maturity at ca. two years, and length at 50% maturity at 259-290 mm FL depending on growth rate. Spawning season occurred in the spring months of August to October. The Wriggleswade Dam population reached similar ages (14 yrs) to populations in temperate North America. Growth was described using the von Bertalanffy growth equations of (Lt = 420(1-ᵉ⁻°·³³⁽ᵗ⁺°·²¹⁾) mm FL in Wriggleswade and (Lt = 641(1-ᵉ⁻°·²²⁽ᵗ⁺°·⁷⁶⁾) mm FL in Mankazana. Growth performance of both populations, described using the phi prime index, seemed to be correlated with temperature and the Wriggleswade Dam population (Φ’ = 2.8) grew similarly to those native temperate North American populations and the non-native populations of Japan and Italy. The Mankazana population had a higher growth performance (Φ’ = 2.9) compared to Wriggleswade and growth was comparable to other nonnative African populations. Both populations utilised all available food resources, with the Mankazana population being in better condition than the Wriggleswade population as a result of a more diverse diet, highly abundant food sources throughout the year, and potentially the adoption of a more conservative ambush feeding strategy. The Wriggleswade population, which was utilised by competitive anglers, showed low mortality rates (Z = 0.43 yr⁻¹), and translocated fish dispersed from the common release site, travelling an average distance of 4km over the study time of 494 days. These results indicated that M. salmoides are successful in these environments, growing at expected temperature dependant rates, maturing at two years, using all available food groups, and suffering from low mortality. It is therefore likely that they will persist in South Africa and establish where introduced.
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Book Review: The Hereford Diocesan Guild of Bellringers, 1886-1986: The History of the First Hundred Years by D.John C.Eisel
- Authors: Lewis, Colin A
- Date: 2013
- Language: English
- Type: Book review
- Identifier: vital:6160 , http://hdl.handle.net/10962/d1004780 , http://ringingworld.co.uk
- Description: The Hereford DGBR was founded in 1886, mainly at the instigation of two clergymen within the diocese: Rev G. M.Custance of Colwall and Prebendary W. H. Phillott, who was sometime incumbent of Staunton-on-Wye. John Eisel's book traces the formation and development of the Guild. The book also indicates the standard of ringing within the diocesan area before the foundation of the Guild.
- Full Text:
- Authors: Lewis, Colin A
- Date: 2013
- Language: English
- Type: Book review
- Identifier: vital:6160 , http://hdl.handle.net/10962/d1004780 , http://ringingworld.co.uk
- Description: The Hereford DGBR was founded in 1886, mainly at the instigation of two clergymen within the diocese: Rev G. M.Custance of Colwall and Prebendary W. H. Phillott, who was sometime incumbent of Staunton-on-Wye. John Eisel's book traces the formation and development of the Guild. The book also indicates the standard of ringing within the diocesan area before the foundation of the Guild.
- Full Text:
Born free without a cause?: Young and mediated
- Authors: Malila, Vanessa
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/158609 , vital:40211 , https://hdl.handle.net/10520/EJC141606
- Description: Each year on the 16th June we celebrate Youth Day and I wonder what the day means to young South Africans. Countries all over the world celebrate Youth Day as a way to highlight the importance of young people in society. In South Africa, it is this and much more. Here this specific day was chosen to commemorate the Soweto Uprising of 1976, when young South Africans rose up against the inequalities, atrocities and injustices of the apartheid government.
- Full Text:
- Authors: Malila, Vanessa
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/158609 , vital:40211 , https://hdl.handle.net/10520/EJC141606
- Description: Each year on the 16th June we celebrate Youth Day and I wonder what the day means to young South Africans. Countries all over the world celebrate Youth Day as a way to highlight the importance of young people in society. In South Africa, it is this and much more. Here this specific day was chosen to commemorate the Soweto Uprising of 1976, when young South Africans rose up against the inequalities, atrocities and injustices of the apartheid government.
- Full Text:
Case study : using visual representations to enhance conceptual knowledge of division in mathematics
- Authors: Joel, Linea Beautty
- Date: 2013
- Subjects: Mathematics -- Study and teaching , Visual learning , Division -- Study and teaching
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1992 , http://hdl.handle.net/10962/d1013356
- Description: Literature emphasizes how important it is that procedural and conceptual knowledge of mathematics should be learned in integration. Yet, generally, the learning and teaching in mathematics classrooms relies heavily on isolated procedures. This study aims to improve teaching and learning of partitive and quotitive division, moving away from isolated procedural knowledge to that of procedures with their underlying concepts through the use of manipulatives, visual representation and questioning. Learning and teaching lessons were designed to teach partitive and quotitive division both procedurally and conceptually. The study explored the roles these manipulatives, visual representations and questioning played toward the conceptual learning of partitive and quotitive division. It was found that manipulatives and iconic visualization enhanced learning, and this could be achieved through scaffolding using a questioning approach. It was concluded that manipulatives and iconic visualization need to be properly planned and used, and integrated with questioning to achieve success in the learning of procedural and conceptual knowledge.
- Full Text:
Case study : using visual representations to enhance conceptual knowledge of division in mathematics
- Authors: Joel, Linea Beautty
- Date: 2013
- Subjects: Mathematics -- Study and teaching , Visual learning , Division -- Study and teaching
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1992 , http://hdl.handle.net/10962/d1013356
- Description: Literature emphasizes how important it is that procedural and conceptual knowledge of mathematics should be learned in integration. Yet, generally, the learning and teaching in mathematics classrooms relies heavily on isolated procedures. This study aims to improve teaching and learning of partitive and quotitive division, moving away from isolated procedural knowledge to that of procedures with their underlying concepts through the use of manipulatives, visual representation and questioning. Learning and teaching lessons were designed to teach partitive and quotitive division both procedurally and conceptually. The study explored the roles these manipulatives, visual representations and questioning played toward the conceptual learning of partitive and quotitive division. It was found that manipulatives and iconic visualization enhanced learning, and this could be achieved through scaffolding using a questioning approach. It was concluded that manipulatives and iconic visualization need to be properly planned and used, and integrated with questioning to achieve success in the learning of procedural and conceptual knowledge.
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Catalytic oxidation of thioanisole using oxovanadium (IV)‐functionalized electrospun polybenzimidazole nanofibers
- Walmsley, Ryan S, Hlangothi, Percy, Litwinski, Christian, Nyokong, Tebello, Torto, Nelson, Tshentu, Zenixole R
- Authors: Walmsley, Ryan S , Hlangothi, Percy , Litwinski, Christian , Nyokong, Tebello , Torto, Nelson , Tshentu, Zenixole R
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/242083 , vital:51000 , xlink:href="https://doi.org/10.1002/app.38067"
- Description: Polybenzimidazole fibers, with an average diameter of 262 nm, were produced by the process of electrospinning. These fibers were used as a solid support material for the immobilization of oxovanadium(IV) which was achieved via a reaction with vanadyl sulfate. The oxovanadium(IV)-functionalized nanofibers were used as heterogeneous catalysts for the oxidation of thioanisole under both batch and pseudo-continuous flow conditions with great success. Under batch conditions near quantitative oxidation of thioanisole was achieved in under 90 min, even after four successive catalytic reactions. Under continuous conditions, excellent conversion of thioanisole was maintained throughout the period studied at flow rates of up to 2 mLh−1. This study, therefore, proposes that electrospun polybenzimidazole nanofibers, with their small diameters, impressive chemical and thermal stability, as well as coordinating benzimidazole group, may be a desirable support material for immobilization of homogeneous catalysts.
- Full Text:
- Authors: Walmsley, Ryan S , Hlangothi, Percy , Litwinski, Christian , Nyokong, Tebello , Torto, Nelson , Tshentu, Zenixole R
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/242083 , vital:51000 , xlink:href="https://doi.org/10.1002/app.38067"
- Description: Polybenzimidazole fibers, with an average diameter of 262 nm, were produced by the process of electrospinning. These fibers were used as a solid support material for the immobilization of oxovanadium(IV) which was achieved via a reaction with vanadyl sulfate. The oxovanadium(IV)-functionalized nanofibers were used as heterogeneous catalysts for the oxidation of thioanisole under both batch and pseudo-continuous flow conditions with great success. Under batch conditions near quantitative oxidation of thioanisole was achieved in under 90 min, even after four successive catalytic reactions. Under continuous conditions, excellent conversion of thioanisole was maintained throughout the period studied at flow rates of up to 2 mLh−1. This study, therefore, proposes that electrospun polybenzimidazole nanofibers, with their small diameters, impressive chemical and thermal stability, as well as coordinating benzimidazole group, may be a desirable support material for immobilization of homogeneous catalysts.
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Catching on to concatenation : evidence for prepollination intrasexual selection in plants
- Peter, Craig I, Shuttleworth, Adam
- Authors: Peter, Craig I , Shuttleworth, Adam
- Date: 2013
- Language: English
- Type: Article
- Identifier: vital:6560 , http://hdl.handle.net/10962/d1011615 , http://dx.doi.org/10.1111/nph.12838
- Description: [from introduction] Darwin (1871) proposed the mechanism of sexual selection to explain the extreme traits, secondary sex characters, seen primarily in male animals for either display or competition. These elaborate traits for attracting members of the opposite sex or competing for access to mates, inter- and intra-sexual selection, respectively, would increase the mating success of individuals but may reduce individual survival. Sexual selection has traditionally been associated with motile, dioecious animals that have well-developed sensory abilities and has long been viewed as absent as a mode of selection in plants (Grant, 1995). However, since the 1980s, several workers have argued that sexual selection is applicable to plants and should be considered as a factor in floral evolution (Skogsmyr & Lankinen, 2002). Opponents such as Grant (1995) point to incompatibility with Darwin’s usage, hermaphroditism, the absence of obvious secondary sex characters and the apparent absence of female choice in plants (Skogsmyr & Lankinen, 2002; Moore & Pannell, 2011). Proponents, however, argue that historical definitions should be expanded to explain modern evidence and that sexual selection can, in principle, occur in hermaphrodites and hence play a role in the evolution of floral traits. The latter view is supported by a recent study by Cocucci et al. (this issue of New Phytologist, pp. 280–286) which demonstrates intra-sexual selection in plants via direct male–male competition between the pollinaria of milkweeds for optimal attachment sites on pollinators to ensure subsequent pollinium deposition. This is the first example of male–male competition resulting in secondary sex characters in hermaphrodite plants, highlighting the possibility of overlooked mechanisms of sexual selection in plants.
- Full Text:
- Authors: Peter, Craig I , Shuttleworth, Adam
- Date: 2013
- Language: English
- Type: Article
- Identifier: vital:6560 , http://hdl.handle.net/10962/d1011615 , http://dx.doi.org/10.1111/nph.12838
- Description: [from introduction] Darwin (1871) proposed the mechanism of sexual selection to explain the extreme traits, secondary sex characters, seen primarily in male animals for either display or competition. These elaborate traits for attracting members of the opposite sex or competing for access to mates, inter- and intra-sexual selection, respectively, would increase the mating success of individuals but may reduce individual survival. Sexual selection has traditionally been associated with motile, dioecious animals that have well-developed sensory abilities and has long been viewed as absent as a mode of selection in plants (Grant, 1995). However, since the 1980s, several workers have argued that sexual selection is applicable to plants and should be considered as a factor in floral evolution (Skogsmyr & Lankinen, 2002). Opponents such as Grant (1995) point to incompatibility with Darwin’s usage, hermaphroditism, the absence of obvious secondary sex characters and the apparent absence of female choice in plants (Skogsmyr & Lankinen, 2002; Moore & Pannell, 2011). Proponents, however, argue that historical definitions should be expanded to explain modern evidence and that sexual selection can, in principle, occur in hermaphrodites and hence play a role in the evolution of floral traits. The latter view is supported by a recent study by Cocucci et al. (this issue of New Phytologist, pp. 280–286) which demonstrates intra-sexual selection in plants via direct male–male competition between the pollinaria of milkweeds for optimal attachment sites on pollinators to ensure subsequent pollinium deposition. This is the first example of male–male competition resulting in secondary sex characters in hermaphrodite plants, highlighting the possibility of overlooked mechanisms of sexual selection in plants.
- Full Text:
Changes in muscle recruitment, functional strength and ratings of perceived effort during an 8-over bowling spell: impact on performance
- Authors: Barford, Gareth Charles
- Date: 2013
- Subjects: Cricket -- Bowling , Sports -- Physiological aspects , Muscles -- Wounds and injuries , Fatigue , Physical fitness , Cricket injuries
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5102 , http://hdl.handle.net/10962/d1003926 , Cricket -- Bowling , Sports -- Physiological aspects , Muscles -- Wounds and injuries , Fatigue , Physical fitness , Cricket injuries
- Description: Background: The musculoskeletal demands placed on the lower limb musculature of fast bowlers over time have not received much attention. In particular, measures of muscle recruitment changes have, to the author’s knowledge, not been considered. Objective: The present study, therefore sought to establish any associations between an eight over, simulated fast bowling spell, and muscle activation patterns, power output, perceptual demands, and changes in performance. This will enable improvements in the development of training programmes. Methods: Players’ were required to attend two sessions in total. The purpose of the initial session was to collect specific demographic, anthropometric and physiological data and injury history information from each player. In addition, this first session allowed for habituation with the treadmill, the jump meter and all other equipment involved in experimentation. The second testing session involved electrode attachment sites being identified on player’s dominant leg. The areas were then shaved, wiped with an alcohol swab and left to dry, to ensure good connectivity. Pre- and post- measures of muscle activity and functional strength of the lower limbs were recorded in the Department of Human Kinetics and Ergonomics. The protocol took place at the Kingswood High Performance Centre, which is in close proximity to the initial testing site. The protocol involved players bowling eight overs (48 balls). During the protocol, accuracy, ball release speed and perceptual measures were recorded at the end of each over. After the protocol, players were driven back to the Human Kinetics and Ergonomics Department where post-testing measures were completed. The dependable variables of interest were muscle activation, functional strength of the lower limbs, ‘local’ ratings of perceived exertion (RPE), body discomfort, accuracy, and ball release speed. Results: For all muscles it was shown that, as the speed increased so did the muscle activity in players’ lower limbs. There were no significant changes in muscle activity preversus post-protocol. There was however, a general trend of decreasing muscle activity post protocol at higher testing speeds. There were significant (p<0.05) decreases in peak power following the simulated eight over bowling spell. ‘Local’ RPE displayed a significant (P<0.05) increase with each additional over and were observed to reach the ‘heavy’ category. The players’ highest discomfort area was in the lower back, with 13 players perceiving discomfort in this region following the eight over spell. The shoulder and chest were another two areas player’s indicated discomfort with eight players selecting the dominant shoulder. Seven players complained of the dominant side pectoral muscle, leading foot and dominant latissimus dorsi muscle being uncomfortable. Interestingly, the dominant pectoral showed the highest body discomfort ratings amongst players. There were no significant changes in accuracy between overs although there were large interindividual differences in accuracy points between players. The decrease in ball release speed observed during over seven was shown to be significantly (p<0.05) lower than overs one to four. Conclusion: The power output and perceived strain results of the players, appears to indicate the presence of fatigue in players. However, the results are not conclusive, as the fatigue was not shown in muscle recruitment patterns, as well as the body discomfort ratings. There was a non-significant trend observed in the lower limb muscle activation decreasing at higher speeds. Players were able to maintain accuracy. However, the significantly lower ball release speed observed during over seven showed players performance decreasing.
- Full Text:
- Authors: Barford, Gareth Charles
- Date: 2013
- Subjects: Cricket -- Bowling , Sports -- Physiological aspects , Muscles -- Wounds and injuries , Fatigue , Physical fitness , Cricket injuries
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5102 , http://hdl.handle.net/10962/d1003926 , Cricket -- Bowling , Sports -- Physiological aspects , Muscles -- Wounds and injuries , Fatigue , Physical fitness , Cricket injuries
- Description: Background: The musculoskeletal demands placed on the lower limb musculature of fast bowlers over time have not received much attention. In particular, measures of muscle recruitment changes have, to the author’s knowledge, not been considered. Objective: The present study, therefore sought to establish any associations between an eight over, simulated fast bowling spell, and muscle activation patterns, power output, perceptual demands, and changes in performance. This will enable improvements in the development of training programmes. Methods: Players’ were required to attend two sessions in total. The purpose of the initial session was to collect specific demographic, anthropometric and physiological data and injury history information from each player. In addition, this first session allowed for habituation with the treadmill, the jump meter and all other equipment involved in experimentation. The second testing session involved electrode attachment sites being identified on player’s dominant leg. The areas were then shaved, wiped with an alcohol swab and left to dry, to ensure good connectivity. Pre- and post- measures of muscle activity and functional strength of the lower limbs were recorded in the Department of Human Kinetics and Ergonomics. The protocol took place at the Kingswood High Performance Centre, which is in close proximity to the initial testing site. The protocol involved players bowling eight overs (48 balls). During the protocol, accuracy, ball release speed and perceptual measures were recorded at the end of each over. After the protocol, players were driven back to the Human Kinetics and Ergonomics Department where post-testing measures were completed. The dependable variables of interest were muscle activation, functional strength of the lower limbs, ‘local’ ratings of perceived exertion (RPE), body discomfort, accuracy, and ball release speed. Results: For all muscles it was shown that, as the speed increased so did the muscle activity in players’ lower limbs. There were no significant changes in muscle activity preversus post-protocol. There was however, a general trend of decreasing muscle activity post protocol at higher testing speeds. There were significant (p<0.05) decreases in peak power following the simulated eight over bowling spell. ‘Local’ RPE displayed a significant (P<0.05) increase with each additional over and were observed to reach the ‘heavy’ category. The players’ highest discomfort area was in the lower back, with 13 players perceiving discomfort in this region following the eight over spell. The shoulder and chest were another two areas player’s indicated discomfort with eight players selecting the dominant shoulder. Seven players complained of the dominant side pectoral muscle, leading foot and dominant latissimus dorsi muscle being uncomfortable. Interestingly, the dominant pectoral showed the highest body discomfort ratings amongst players. There were no significant changes in accuracy between overs although there were large interindividual differences in accuracy points between players. The decrease in ball release speed observed during over seven was shown to be significantly (p<0.05) lower than overs one to four. Conclusion: The power output and perceived strain results of the players, appears to indicate the presence of fatigue in players. However, the results are not conclusive, as the fatigue was not shown in muscle recruitment patterns, as well as the body discomfort ratings. There was a non-significant trend observed in the lower limb muscle activation decreasing at higher speeds. Players were able to maintain accuracy. However, the significantly lower ball release speed observed during over seven showed players performance decreasing.
- Full Text:
Characterisation of a plasmodium falciparum type II Hsp40 chaperone exported to the cytosol of infected erythrocytes
- Maphumulo, Philile Nompumelelo
- Authors: Maphumulo, Philile Nompumelelo
- Date: 2013
- Subjects: Erythrocytes , Heat shock proteins , Plasmodium falciparum , Molecular chaperones , Malaria -- Prevention -- Research , Protein folding , Proteins -- Analysis , Malaria -- Immunological aspects
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4128 , http://hdl.handle.net/10962/d1015681
- Description: Heat Shock 40 kDa proteins (Hsp40s) partner with heat shock 70 kDa proteins (Hsp70s) in facilitating, among other chaperone activities; correct protein transport, productive protein folding and assembly within the cells; under both normal and stressful conditions. Hsp40 proteins regulate the ATPase activity of Hsp70 through interaction with the J-domain. Plasmodium falciparum Hsp70s (PfHsp70s) do not contain a Plasmodium export element (PEXEL) sequence although PfHsp70-1 and PfHsp70-3 have been located outside of the parasitophorous vacuole. Studies reveal that a type I P. falciparum (PfHsp40) chaperone (PF14_0359) stimulates the rate of ATP hydrolysis of the cytosolic PfHsp70 (PfHsp70-1) and that of human Hsp70A1A. PFE0055c is a PEXEL-bearing type II Hsp40 that is exported into the cytosol of P. falciparum-infected erythrocytes; where it potentially interacts with human Hsp70. Studies reveal that PFE0055c associates with structures found in the erythrocyte cytosol termed “J-dots” which are believed to be involved in trafficking parasite-encoded proteins through the erythrocyte cytosol. If P. falciparum exports PFE0055c into the host cytosol, it may be proposed that it interacts with human Hsp70, making it a possible drug target. The effect of PFE0055c on the ATPase activity of human Hsp70A1A has not been previously characterised. Central to this study was bioinformatic analysis and biochemical characterisation PFE0055c using an in vitro (ATPase assay) approach. Structural domains that classify PFE0055c as a type II Hsp40 were identified with similarity to two other exported type II PfHsp40s. Plasmids encoding the hexahistidine-tagged versions of PFE0055c and human Hsp70A1A were used for the expression and purification of these proteins from Escherichia coli. Purification was achieved using nickel affinity chromatography. The urea-denaturing method was used to obtain the purified PFE0055c whilst human Hsp70A1A was purified using the native method. PFE0055c could stimulate the ATPase activity of alfalfa Hsp70, although such was not the case for human Hsp70A1A in vitro.
- Full Text:
- Authors: Maphumulo, Philile Nompumelelo
- Date: 2013
- Subjects: Erythrocytes , Heat shock proteins , Plasmodium falciparum , Molecular chaperones , Malaria -- Prevention -- Research , Protein folding , Proteins -- Analysis , Malaria -- Immunological aspects
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4128 , http://hdl.handle.net/10962/d1015681
- Description: Heat Shock 40 kDa proteins (Hsp40s) partner with heat shock 70 kDa proteins (Hsp70s) in facilitating, among other chaperone activities; correct protein transport, productive protein folding and assembly within the cells; under both normal and stressful conditions. Hsp40 proteins regulate the ATPase activity of Hsp70 through interaction with the J-domain. Plasmodium falciparum Hsp70s (PfHsp70s) do not contain a Plasmodium export element (PEXEL) sequence although PfHsp70-1 and PfHsp70-3 have been located outside of the parasitophorous vacuole. Studies reveal that a type I P. falciparum (PfHsp40) chaperone (PF14_0359) stimulates the rate of ATP hydrolysis of the cytosolic PfHsp70 (PfHsp70-1) and that of human Hsp70A1A. PFE0055c is a PEXEL-bearing type II Hsp40 that is exported into the cytosol of P. falciparum-infected erythrocytes; where it potentially interacts with human Hsp70. Studies reveal that PFE0055c associates with structures found in the erythrocyte cytosol termed “J-dots” which are believed to be involved in trafficking parasite-encoded proteins through the erythrocyte cytosol. If P. falciparum exports PFE0055c into the host cytosol, it may be proposed that it interacts with human Hsp70, making it a possible drug target. The effect of PFE0055c on the ATPase activity of human Hsp70A1A has not been previously characterised. Central to this study was bioinformatic analysis and biochemical characterisation PFE0055c using an in vitro (ATPase assay) approach. Structural domains that classify PFE0055c as a type II Hsp40 were identified with similarity to two other exported type II PfHsp40s. Plasmids encoding the hexahistidine-tagged versions of PFE0055c and human Hsp70A1A were used for the expression and purification of these proteins from Escherichia coli. Purification was achieved using nickel affinity chromatography. The urea-denaturing method was used to obtain the purified PFE0055c whilst human Hsp70A1A was purified using the native method. PFE0055c could stimulate the ATPase activity of alfalfa Hsp70, although such was not the case for human Hsp70A1A in vitro.
- Full Text:
Characterisation of novel CrleGV isolates for false codling moth control-lessons learnt from codling moth resistance to CpGV. Characterisation of novel CrleGV isolates for false codling moth control-lessons learnt from codling moth resistance to CpGV
- Opoku-Debrah, John K, Moore, Sean D, Hill, Martin P, Knox, Caroline M
- Authors: Opoku-Debrah, John K , Moore, Sean D , Hill, Martin P , Knox, Caroline M
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/425414 , vital:72237 , xlink:href="https://www.cabdirect.org/cabdirect/abstract/20133257674"
- Description: Recently some codling moth, Cydia pomonella, populations in Europe developed resistance to CpGV. In order to prepare for the possibility of a similar occurrence with the false codling moth, Thaumatotibia leucotreta, in South Africa, a search was conducted for novel CrleGV isolates. Through overcrowding, outbreaks of novel isolates were recorded from laboratory populations of five geographically distinct host populations. The genetic novelty of these and two commercially available isolates was confirmed through restriction enzyme analysis and sequence analysis of the granulin and egt genes. Phylogenetic analysis showed the existence of two CrleGV-SA genome types. Significant differences in virulence were also shown between certain isolates against certain host populations.
- Full Text:
- Authors: Opoku-Debrah, John K , Moore, Sean D , Hill, Martin P , Knox, Caroline M
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/425414 , vital:72237 , xlink:href="https://www.cabdirect.org/cabdirect/abstract/20133257674"
- Description: Recently some codling moth, Cydia pomonella, populations in Europe developed resistance to CpGV. In order to prepare for the possibility of a similar occurrence with the false codling moth, Thaumatotibia leucotreta, in South Africa, a search was conducted for novel CrleGV isolates. Through overcrowding, outbreaks of novel isolates were recorded from laboratory populations of five geographically distinct host populations. The genetic novelty of these and two commercially available isolates was confirmed through restriction enzyme analysis and sequence analysis of the granulin and egt genes. Phylogenetic analysis showed the existence of two CrleGV-SA genome types. Significant differences in virulence were also shown between certain isolates against certain host populations.
- Full Text:
Characterisation of surfaces modified through self-assembled monolayers and click chemistry
- Authors: Coates, Megan Patricia
- Date: 2013
- Subjects: Monomolecular films Gold Adsorption Nanotubes Self-assembly (Chemistry) Self-assembly (Chemistry) Scanning electrochemical microscopy X-ray photoelectron spectroscopy
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4277 , http://hdl.handle.net/10962/d1001684
- Description: Different approaches to surface modification were investigated in this work on gold, glassy carbon, multi-walled carbon nanotube paper and on single-walled carbon nanotubes adsorbed on glassy carbon. These approaches include electrochemical grafting, electropolymerisation, click chemistry, axial ligation, adsorption and self-assembled monolayers. The modified surfaces were characterised using a variety of techniques; predominantly electrochemistry, scanning electrochemical microscopy and X-ray photoelectron spectroscopy. For the formation of self-assembled monolayers on gold, four new manganese(III) phthalocyanines (1a-d), octa-substituted at the peripheral position with pentylthio, decylthio, benzylthio, and phenylthio groups were synthesized and characterised. X-ray photoelectron spectroscopy was used to show the formation of a sulphur-gold bond. A number of approaches using 4-azidoaniline (2a) combined with azide-alkyne click chemistry and electrochemistry were also used to anchor ferrocene and pyridine moieties on to the carbon surfaces, including direct in situ diazotation and grafting, electropolymerisation, and the synthesis of the diazonium salt followed by grafting. Iron phthalocyanine was linked to the pyridine-clicked surfaces through axial ligation, where the strong axial bond formed by the interaction between the central metal and the lone pair of the nitrogen in the pyridine group resulted in stable modified electrodes. The potential of these surfaces for the detection of analytes such as thiocyanate, hydrazine and sulphite are briefly shown as well. This work also describes for the first time the possibility of performing local micro-electrochemical grafting of a gold substrate by 4-azidobenzenediazonium (2b) using scanning electrochemical microscopy in a single and simple one step approach, without complications from adsorption.
- Full Text:
- Authors: Coates, Megan Patricia
- Date: 2013
- Subjects: Monomolecular films Gold Adsorption Nanotubes Self-assembly (Chemistry) Self-assembly (Chemistry) Scanning electrochemical microscopy X-ray photoelectron spectroscopy
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4277 , http://hdl.handle.net/10962/d1001684
- Description: Different approaches to surface modification were investigated in this work on gold, glassy carbon, multi-walled carbon nanotube paper and on single-walled carbon nanotubes adsorbed on glassy carbon. These approaches include electrochemical grafting, electropolymerisation, click chemistry, axial ligation, adsorption and self-assembled monolayers. The modified surfaces were characterised using a variety of techniques; predominantly electrochemistry, scanning electrochemical microscopy and X-ray photoelectron spectroscopy. For the formation of self-assembled monolayers on gold, four new manganese(III) phthalocyanines (1a-d), octa-substituted at the peripheral position with pentylthio, decylthio, benzylthio, and phenylthio groups were synthesized and characterised. X-ray photoelectron spectroscopy was used to show the formation of a sulphur-gold bond. A number of approaches using 4-azidoaniline (2a) combined with azide-alkyne click chemistry and electrochemistry were also used to anchor ferrocene and pyridine moieties on to the carbon surfaces, including direct in situ diazotation and grafting, electropolymerisation, and the synthesis of the diazonium salt followed by grafting. Iron phthalocyanine was linked to the pyridine-clicked surfaces through axial ligation, where the strong axial bond formed by the interaction between the central metal and the lone pair of the nitrogen in the pyridine group resulted in stable modified electrodes. The potential of these surfaces for the detection of analytes such as thiocyanate, hydrazine and sulphite are briefly shown as well. This work also describes for the first time the possibility of performing local micro-electrochemical grafting of a gold substrate by 4-azidobenzenediazonium (2b) using scanning electrochemical microscopy in a single and simple one step approach, without complications from adsorption.
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Characterization and application of phthalocyanine-gold nanoparticle conjugates
- Authors: Tombe, Sekai Lana
- Date: 2013
- Subjects: Phthalocyanines , Gold , Zinc , Nanoparticles , Bioconjugates , Photochemistry , Photocatalysis , Electrospinning , Polymers , Pollutants , Phenols , Azo dyes
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4293 , http://hdl.handle.net/10962/d1004517 , Phthalocyanines , Gold , Zinc , Nanoparticles , Bioconjugates , Photochemistry , Photocatalysis , Electrospinning , Polymers , Pollutants , Phenols , Azo dyes
- Description: This work presents the syntheses, photophysical and photochemical characterization of arylthio zinc phthalocyanines and their gold nanoparticle conjugates. Spectroscopic and microscopic studies confirmed the formation of the phthalocyanine-gold nanoparticle conjugates which exhibited enhanced photophysicochemical properties in comparison to the phthalocyanines. The studies showed that the presence of gold nanoparticles significantly lowered fluorescence quantum yields and lifetimes. However, this interaction did not restrict the formation of excited singlet and triplet states and hence the formation of singlet oxygen required for photocatalysis. The conjugates showed significantly higher singlet oxygen quantum yields and therefore enhanced photocatalytic activity compared to the phthalocyanines. The zinc phthalocyanines and their gold nanoparticle conjugates were successfully incorporated into electrospun polymer fibers. Spectral characteristics of the functionalized electrospun fibers indicated that the phthalocyanines and phthalocyanine-gold nanoparticle conjugates were bound and their integrity was maintained within the polymeric fiber matrices. The photophysical and photochemical properties of the complexes were equally maintained within the electrospun fibers. The functionalized fibers were applied for the photoconversion of 4-chlorophenol and Orange G as model organic pollutants. , Microsoft� Word 2010 , Adobe Acrobat 9.53 Paper Capture Plug-in
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- Authors: Tombe, Sekai Lana
- Date: 2013
- Subjects: Phthalocyanines , Gold , Zinc , Nanoparticles , Bioconjugates , Photochemistry , Photocatalysis , Electrospinning , Polymers , Pollutants , Phenols , Azo dyes
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4293 , http://hdl.handle.net/10962/d1004517 , Phthalocyanines , Gold , Zinc , Nanoparticles , Bioconjugates , Photochemistry , Photocatalysis , Electrospinning , Polymers , Pollutants , Phenols , Azo dyes
- Description: This work presents the syntheses, photophysical and photochemical characterization of arylthio zinc phthalocyanines and their gold nanoparticle conjugates. Spectroscopic and microscopic studies confirmed the formation of the phthalocyanine-gold nanoparticle conjugates which exhibited enhanced photophysicochemical properties in comparison to the phthalocyanines. The studies showed that the presence of gold nanoparticles significantly lowered fluorescence quantum yields and lifetimes. However, this interaction did not restrict the formation of excited singlet and triplet states and hence the formation of singlet oxygen required for photocatalysis. The conjugates showed significantly higher singlet oxygen quantum yields and therefore enhanced photocatalytic activity compared to the phthalocyanines. The zinc phthalocyanines and their gold nanoparticle conjugates were successfully incorporated into electrospun polymer fibers. Spectral characteristics of the functionalized electrospun fibers indicated that the phthalocyanines and phthalocyanine-gold nanoparticle conjugates were bound and their integrity was maintained within the polymeric fiber matrices. The photophysical and photochemical properties of the complexes were equally maintained within the electrospun fibers. The functionalized fibers were applied for the photoconversion of 4-chlorophenol and Orange G as model organic pollutants. , Microsoft� Word 2010 , Adobe Acrobat 9.53 Paper Capture Plug-in
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Characterization and electrocatalytic behaviour of glassy carbon electrode modified with nickel nanoparticles towards amitrole detection
- Maringa, Audacity, Mugadza, Tawanda, Antunes, Edith M, Nyokong, Tebello
- Authors: Maringa, Audacity , Mugadza, Tawanda , Antunes, Edith M , Nyokong, Tebello
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/241793 , vital:50970 , xlink:href="https://doi.org/10.1016/j.jelechem.2013.04.022"
- Description: We report on the synthesis of Ni nanoparticles (NiNPs) and their application in electrocatalysis in comparison with nickel phthalocyanine (NiPc). UV–vis spectroscopy, powder X-ray diffraction, transmission electron microscopy and electron paramagnetic resonance were used in the characterization of NiNPs. Cyclic voltammetry and electrochemical impedance spectroscopy were used in electrocatalytic studies of amitrole on the glassy carbon electrode modified with NiNPs. The apparent and catalytic rate constants for amitrole on the NiNP-GCE were found to be 2.58 × 10−5 cm s−1 and 1.11 × 103 M−1 s−1, respectively.
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- Authors: Maringa, Audacity , Mugadza, Tawanda , Antunes, Edith M , Nyokong, Tebello
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/241793 , vital:50970 , xlink:href="https://doi.org/10.1016/j.jelechem.2013.04.022"
- Description: We report on the synthesis of Ni nanoparticles (NiNPs) and their application in electrocatalysis in comparison with nickel phthalocyanine (NiPc). UV–vis spectroscopy, powder X-ray diffraction, transmission electron microscopy and electron paramagnetic resonance were used in the characterization of NiNPs. Cyclic voltammetry and electrochemical impedance spectroscopy were used in electrocatalytic studies of amitrole on the glassy carbon electrode modified with NiNPs. The apparent and catalytic rate constants for amitrole on the NiNP-GCE were found to be 2.58 × 10−5 cm s−1 and 1.11 × 103 M−1 s−1, respectively.
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Characterization and photophysical behavior of phthalocyanines when grafted onto silica nanoparticles
- Fashina, Adedayo, Antunes, Edith M, Nyokong, Tebello
- Authors: Fashina, Adedayo , Antunes, Edith M , Nyokong, Tebello
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/241981 , vital:50989 , xlink:href="https://doi.org/10.1016/j.poly.2013.01.037"
- Description: This work reports on the functionalization of silica nanoparticles with zinc phthalocyanine complexes tetra-substituted non-peripherally with 4-carboxyphenoxyl and 3-carboxyphenoxyl groups. The phthalocyanine dyes have a free carboxyl group, facilitating the covalent attachment of the dyes on the silica surface via amide bond formation. The phthalocyanine functionalized silica nanoparticles showed higher fluorescence and triplet quantum yields as well as longer triplet lifetimes compared to the free phthalocyanines.
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- Authors: Fashina, Adedayo , Antunes, Edith M , Nyokong, Tebello
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/241981 , vital:50989 , xlink:href="https://doi.org/10.1016/j.poly.2013.01.037"
- Description: This work reports on the functionalization of silica nanoparticles with zinc phthalocyanine complexes tetra-substituted non-peripherally with 4-carboxyphenoxyl and 3-carboxyphenoxyl groups. The phthalocyanine dyes have a free carboxyl group, facilitating the covalent attachment of the dyes on the silica surface via amide bond formation. The phthalocyanine functionalized silica nanoparticles showed higher fluorescence and triplet quantum yields as well as longer triplet lifetimes compared to the free phthalocyanines.
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