Molecular characterization of the Mycobacterium tuberculosis complex (MTC) of raw milk from selected dairy farms in the Eastern Cape
- Authors: Komani, Nosiphiwo
- Date: 2013
- Subjects: Mycobacterium tuberculosis -- South Africa -- Eastern Cape , Milk -- South Africa -- Eastern Cape , Dairy farms -- South Africa -- Eastern Cape , Dairy farming -- South Africa -- Eastern Cape , Tuberculosis in cattle -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11276 , http://hdl.handle.net/10353/d1013157 , Mycobacterium tuberculosis -- South Africa -- Eastern Cape , Milk -- South Africa -- Eastern Cape , Dairy farms -- South Africa -- Eastern Cape , Dairy farming -- South Africa -- Eastern Cape , Tuberculosis in cattle -- South Africa -- Eastern Cape
- Description: Tuberculosis (TB) is an ancient infectious disease that has been infecting different populations around the globe and it has also been considered as one of the most successful human and animal disease. TB found in animals such as cattle and other known bovids is known as bovine tuberculosis. Bovine tuberculosis (BTB) is an infectious disease found in cattle mainly caused by Mycobacterium bovis. M. bovis is a member of the Mycobacterium tuberculosis complex (MTC) together with M. tuberculosis, M. africanum, and M. canetti where the natural host is humans; whereas M. caprae, M. microti and M. pinnipedii usually have animals as their natural host. In this study the molecular characterization of the MTC from cow milk in the Eastern Cape was investigated. One hundred and twenty samples (40 ml each) were collected from three dairy farms in the Eastern Cape, South Africa. These samples were processed using a modified Petroff decontamination method. Sample processing was followed by DNA isolation using a Zymo Bacterial/Fungal DNA Kit and the amplification and detection of the MTC was done using the Seeplex MTB Nested ACE assay. The drug susceptibility tests were done using GenoTypeMTBDRplus assay which detects mutations and resistance to INH (isoniazid) and RMP (rifampicin). The milk isolates were further analyzed using a spoligotyping method which is based on the PCR amplification of a highly polymorphic direct repeat locus in the M. tuberculosis genome which detects and types the MTC. A percentage of 20.8 % samples were found to be positive for MTC using the Seeplex MTB Nested ACE assay. There were 42.1 % samples that were resistant to both INH and RMP with the rest sensitive to either INH or RMP. The spoligotyping method showed that 78.3 % samples resembled Family 33 strains and the rest (21.7 %) resembled a spoligotyping signature known to be that of M.africanum. Both these strains belong to the Ancestral lineage with Indo-Oceanic and West Africa 2 lineage. The outcomes of our study showed that molecular methods for detection of MTC can be applied directly on milk samples without the need for culturing.
- Full Text:
- Date Issued: 2013
- Authors: Komani, Nosiphiwo
- Date: 2013
- Subjects: Mycobacterium tuberculosis -- South Africa -- Eastern Cape , Milk -- South Africa -- Eastern Cape , Dairy farms -- South Africa -- Eastern Cape , Dairy farming -- South Africa -- Eastern Cape , Tuberculosis in cattle -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc (Microbiology)
- Identifier: vital:11276 , http://hdl.handle.net/10353/d1013157 , Mycobacterium tuberculosis -- South Africa -- Eastern Cape , Milk -- South Africa -- Eastern Cape , Dairy farms -- South Africa -- Eastern Cape , Dairy farming -- South Africa -- Eastern Cape , Tuberculosis in cattle -- South Africa -- Eastern Cape
- Description: Tuberculosis (TB) is an ancient infectious disease that has been infecting different populations around the globe and it has also been considered as one of the most successful human and animal disease. TB found in animals such as cattle and other known bovids is known as bovine tuberculosis. Bovine tuberculosis (BTB) is an infectious disease found in cattle mainly caused by Mycobacterium bovis. M. bovis is a member of the Mycobacterium tuberculosis complex (MTC) together with M. tuberculosis, M. africanum, and M. canetti where the natural host is humans; whereas M. caprae, M. microti and M. pinnipedii usually have animals as their natural host. In this study the molecular characterization of the MTC from cow milk in the Eastern Cape was investigated. One hundred and twenty samples (40 ml each) were collected from three dairy farms in the Eastern Cape, South Africa. These samples were processed using a modified Petroff decontamination method. Sample processing was followed by DNA isolation using a Zymo Bacterial/Fungal DNA Kit and the amplification and detection of the MTC was done using the Seeplex MTB Nested ACE assay. The drug susceptibility tests were done using GenoTypeMTBDRplus assay which detects mutations and resistance to INH (isoniazid) and RMP (rifampicin). The milk isolates were further analyzed using a spoligotyping method which is based on the PCR amplification of a highly polymorphic direct repeat locus in the M. tuberculosis genome which detects and types the MTC. A percentage of 20.8 % samples were found to be positive for MTC using the Seeplex MTB Nested ACE assay. There were 42.1 % samples that were resistant to both INH and RMP with the rest sensitive to either INH or RMP. The spoligotyping method showed that 78.3 % samples resembled Family 33 strains and the rest (21.7 %) resembled a spoligotyping signature known to be that of M.africanum. Both these strains belong to the Ancestral lineage with Indo-Oceanic and West Africa 2 lineage. The outcomes of our study showed that molecular methods for detection of MTC can be applied directly on milk samples without the need for culturing.
- Full Text:
- Date Issued: 2013
Molecular signaling in colorectal carcinogenesis : the roles and relationships of beta-catenin, PPARgamma and COX-2
- Authors: Fredericks, Ernst
- Date: 2013
- Subjects: Colon (Anatomy) -- Cancer , Cyclooxygenase 2 , Peroxisomes
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10357 , http://hdl.handle.net/10948/d1021014
- Description: Colorectal cancer (CRC) is a common disease with significant morbidity and mortality. In spite of significant advances in understanding the molecular signaling in this disorder, unanswered questions remain. Cyclooxygenase-2 (COX-2) and β-catenin have established roles in colorectal carcinogenesis, with both being upregulated early in the disease course. The role of peroxisome proliferator-activated receptor γ (PPARγ) is less clear, but has been shown to be downregulated in colon cancer models. Butyrate, a short chain fatty acid, produced by colon microbiota and transported into the colonocyte by transporter proteins, appears to be important in early carcinogenesis. The butyrate concentration is reduced in CRC and so are its transporters. Interleukin-17 (IL-17) plays a role in colitis-associated colorectal cancer (CAC), but its function in sporadic CRC is less clear. Similarly, Protein kinase C (PKC) has proven involvement in many solid tumours, including CRC, but its exact mechanistic role is still speculative. AIM: To investigate the role and possible signaling pathways of the major role players, β-catenin, COX-2 and PPARγ in early CRC. Further, to elucidate the mechanistic pathways of butyrate and its transporters, IL-17 and PKC in CRC. METHOD: Informed consent was obtained for all patients. Patients were recruited in various disease categories, including normal, irritable bowel syndrome (IBS), inflammatory bowel disease (IBD) and CRC. Colon biopsy specimens were obtained during colonoscopy and used for immunohistochemistry (IHC) and gene expression analysis of the above genes by quantitative polymerase chain reaction (qPCR). RESULTS: β-catenin mRNA and protein expression was increased in CRC and the IBD groups compared to the normal group, while it was reduced in the IBS groups. COX-2 mRNA expression showed a steady increase from normal, through IBS, IBD and CRC groups to a statistically significant degree. The COX-2 protein expression, however, did not match the mRNA expression with increased COX-2 protein expression in normal and IBS groups and reduced expression in IBD and CRC groups. PPARγ mRNA expression was unchanged in IBD and CRC groups, but significantly increased in the IBS group compared to normal. Butyrate transporter, SLC16A1 mRNA was significantly reduced in CRC, but also in the IBS groups, which was unexpected. In the IBD group, SLC16A1 mRNA was unchanged in Crohn’s disease (CD) but significantly reduced in ulcerative colitis (UC). Similarly, SLC5A8 mRNA expression was significantly reduced in the CRC as well as the IBS groups. In the IBD groups, SLC5A8 was unchanged in UC but significantly increased in CD. IL-17 mRNA expression was significantly reduced in CRC and IBS groups, but unchanged in the IBD groups. PKCε mRNA was significantly increased in CRC as expected. In the IBD groups, PKCε mRNA was unchanged in CD but significantly increased in UC. In the IBS groups, PKCε mRNA in constipation –IBS (C-IBS) was significantly reduced, but unchanged in diarrhoea – IBS (D-IBS). CONCLUSIONS: β-catenin mRNA and protein expression was increased in CRC and the CRC promoting IBD groups. COX-2 protein expression was incongruent with the COX-2 mRNA expression and this may reflect homeostatic control mechanisms. High COX-2 mRNA expression in CRC and CRC promoting IBD groups may be a secondary phenomenon reflecting the inflammatory milieu, rather than a true carcinogenesis-related event. PPARγ does not appear to play a central role in early colon carcinogenesis, in spite of available literature suggesting otherwise. Butyrate transporters showed inconsistent results and for now no firm conclusions can be drawn from this. IL-17 may play a role in CAC as confirmed in this and other studies, but its role in sporadic CRC is tenuous and requires further investigation. Likewise for PKCε, upregulation is associated with increased tumourigenecity as shown in this study, however, the mechanistic pathway(s) involved is still speculative and requires further study.
- Full Text:
- Date Issued: 2013
- Authors: Fredericks, Ernst
- Date: 2013
- Subjects: Colon (Anatomy) -- Cancer , Cyclooxygenase 2 , Peroxisomes
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10357 , http://hdl.handle.net/10948/d1021014
- Description: Colorectal cancer (CRC) is a common disease with significant morbidity and mortality. In spite of significant advances in understanding the molecular signaling in this disorder, unanswered questions remain. Cyclooxygenase-2 (COX-2) and β-catenin have established roles in colorectal carcinogenesis, with both being upregulated early in the disease course. The role of peroxisome proliferator-activated receptor γ (PPARγ) is less clear, but has been shown to be downregulated in colon cancer models. Butyrate, a short chain fatty acid, produced by colon microbiota and transported into the colonocyte by transporter proteins, appears to be important in early carcinogenesis. The butyrate concentration is reduced in CRC and so are its transporters. Interleukin-17 (IL-17) plays a role in colitis-associated colorectal cancer (CAC), but its function in sporadic CRC is less clear. Similarly, Protein kinase C (PKC) has proven involvement in many solid tumours, including CRC, but its exact mechanistic role is still speculative. AIM: To investigate the role and possible signaling pathways of the major role players, β-catenin, COX-2 and PPARγ in early CRC. Further, to elucidate the mechanistic pathways of butyrate and its transporters, IL-17 and PKC in CRC. METHOD: Informed consent was obtained for all patients. Patients were recruited in various disease categories, including normal, irritable bowel syndrome (IBS), inflammatory bowel disease (IBD) and CRC. Colon biopsy specimens were obtained during colonoscopy and used for immunohistochemistry (IHC) and gene expression analysis of the above genes by quantitative polymerase chain reaction (qPCR). RESULTS: β-catenin mRNA and protein expression was increased in CRC and the IBD groups compared to the normal group, while it was reduced in the IBS groups. COX-2 mRNA expression showed a steady increase from normal, through IBS, IBD and CRC groups to a statistically significant degree. The COX-2 protein expression, however, did not match the mRNA expression with increased COX-2 protein expression in normal and IBS groups and reduced expression in IBD and CRC groups. PPARγ mRNA expression was unchanged in IBD and CRC groups, but significantly increased in the IBS group compared to normal. Butyrate transporter, SLC16A1 mRNA was significantly reduced in CRC, but also in the IBS groups, which was unexpected. In the IBD group, SLC16A1 mRNA was unchanged in Crohn’s disease (CD) but significantly reduced in ulcerative colitis (UC). Similarly, SLC5A8 mRNA expression was significantly reduced in the CRC as well as the IBS groups. In the IBD groups, SLC5A8 was unchanged in UC but significantly increased in CD. IL-17 mRNA expression was significantly reduced in CRC and IBS groups, but unchanged in the IBD groups. PKCε mRNA was significantly increased in CRC as expected. In the IBD groups, PKCε mRNA was unchanged in CD but significantly increased in UC. In the IBS groups, PKCε mRNA in constipation –IBS (C-IBS) was significantly reduced, but unchanged in diarrhoea – IBS (D-IBS). CONCLUSIONS: β-catenin mRNA and protein expression was increased in CRC and the CRC promoting IBD groups. COX-2 protein expression was incongruent with the COX-2 mRNA expression and this may reflect homeostatic control mechanisms. High COX-2 mRNA expression in CRC and CRC promoting IBD groups may be a secondary phenomenon reflecting the inflammatory milieu, rather than a true carcinogenesis-related event. PPARγ does not appear to play a central role in early colon carcinogenesis, in spite of available literature suggesting otherwise. Butyrate transporters showed inconsistent results and for now no firm conclusions can be drawn from this. IL-17 may play a role in CAC as confirmed in this and other studies, but its role in sporadic CRC is tenuous and requires further investigation. Likewise for PKCε, upregulation is associated with increased tumourigenecity as shown in this study, however, the mechanistic pathway(s) involved is still speculative and requires further study.
- Full Text:
- Date Issued: 2013
Monetary policy in Namibia, 1993-2011
- Sheefeni, Johannes Peyavali Sheefeni
- Authors: Sheefeni, Johannes Peyavali Sheefeni
- Date: 2013
- Subjects: Monetary policy -- Namibia , Transmission mechanism (Monetary policy) -- Namibia , Money supply -- Namibia
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/3935 , vital:20493
- Description: This thesis investigated the role of monetary policy in Namibia for the period 1993 to 2011. It aims at achieving six objectives. First, it reviews the evolution of monetary policy in Namibia for the period 1980 to 2011. Second, it investigates the interest rate channel of the monetary policy transmission mechanism in Namibia. Third, it analyses the credit channel of the monetary policy transmission mechanism in Namibia. Fourth, it evaluates the exchange rate channel of the monetary policy transmission mechanism in Namibia. Fifth, it studies the money effect model in the context of the monetary policy transmission mechanism in Namibia. Sixth, it examines the exchange rate pass–through (ERPT) to domestic prices in Namibia. In order to achieve the objectives of the relative importance of the different channels of monetary policy transmission, a structural vector autoregressive model of the Namibian economy is constructed. Specifically the responses of the output and prices to monetary policy shocks for Namibia over the quarterly period 1993:Q1 to 2011:Q4 are investigated using impulse response functions and forecast variance error decompositions obtained from a structural vector autoregressive model (SVAR). The thesis also examined the exchange rate pass-through from exchange rate to domestic prices using both SVAR and the single equation error correction model (ECM). Estimation results on the different channels of monetary policy transmission mechanism showed that the interest rate channel and the credit channel are effective in transmitting monetary policy actions. The exchange rate channel is also operative but not effective. The money effect model confirms that inflation in Namibia is not a monetary phenomenon. The results of the pass-through relationship showed that there is an incomplete but high exchange rate pass-through from exchange rate to domestic prices.
- Full Text:
- Date Issued: 2013
- Authors: Sheefeni, Johannes Peyavali Sheefeni
- Date: 2013
- Subjects: Monetary policy -- Namibia , Transmission mechanism (Monetary policy) -- Namibia , Money supply -- Namibia
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: http://hdl.handle.net/10948/3935 , vital:20493
- Description: This thesis investigated the role of monetary policy in Namibia for the period 1993 to 2011. It aims at achieving six objectives. First, it reviews the evolution of monetary policy in Namibia for the period 1980 to 2011. Second, it investigates the interest rate channel of the monetary policy transmission mechanism in Namibia. Third, it analyses the credit channel of the monetary policy transmission mechanism in Namibia. Fourth, it evaluates the exchange rate channel of the monetary policy transmission mechanism in Namibia. Fifth, it studies the money effect model in the context of the monetary policy transmission mechanism in Namibia. Sixth, it examines the exchange rate pass–through (ERPT) to domestic prices in Namibia. In order to achieve the objectives of the relative importance of the different channels of monetary policy transmission, a structural vector autoregressive model of the Namibian economy is constructed. Specifically the responses of the output and prices to monetary policy shocks for Namibia over the quarterly period 1993:Q1 to 2011:Q4 are investigated using impulse response functions and forecast variance error decompositions obtained from a structural vector autoregressive model (SVAR). The thesis also examined the exchange rate pass-through from exchange rate to domestic prices using both SVAR and the single equation error correction model (ECM). Estimation results on the different channels of monetary policy transmission mechanism showed that the interest rate channel and the credit channel are effective in transmitting monetary policy actions. The exchange rate channel is also operative but not effective. The money effect model confirms that inflation in Namibia is not a monetary phenomenon. The results of the pass-through relationship showed that there is an incomplete but high exchange rate pass-through from exchange rate to domestic prices.
- Full Text:
- Date Issued: 2013
Money supply endogeneity : an empirical investigation of South African data (2000Q1-2011Q4)
- Authors: Schady, Stuart William
- Date: 2013 , 2013-04-29
- Subjects: Monetary policy -- South Africa , Inflation targeting -- South Africa , Gross domestic product -- South Africa , Bank loans -- South Africa , Money supply -- South Africa , Endogenous growth (Economics) , Domestic credit extension
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:928 , http://hdl.handle.net/10962/d1001454 , Monetary policy -- South Africa , Inflation targeting -- South Africa , Gross domestic product -- South Africa , Bank loans -- South Africa , Money supply -- South Africa , Endogenous growth (Economics)
- Description: This study is about whether the money supply in South Africa under a monetary policy regime of inflation‐targeting is exogenously or endogenously determined. The proposition of an exogenous money supply has been offered by monetarists, where the Central Bank determines the quantity of money supplied to the economy and this has a causal influence on income and credit extension. The endogenous money theory is a post‐Keynesian proposition whereby the money creation is determined by banks adjusting their responses to demands for credit‐money from economic agents. The data analysis is from 2000Q1 to 2010Q4 and entails the use of the variables monetary base (MB), domestic credit extension (DCE), M3, and gross national product (GDP). All variables are logged. The empirical tests conducted start with the Augmented Dickey‐Fuller unit root test to determine the variables order of integration. Johansen cointegration tests are done followed by Vector Error‐Correction Models (VECMs) and Granger causality tests to determine whether there is unidirectional or bidirectional causality between variables over the long and short‐run. Based on the results of the testing it was discovered that over the inflation‐targeting regime money supply in South Africa was endogenously determined. Furthermore, the data best supports the Accommodationist analysis of endogenous money as opposed to that of Structuralism and Liquidity Preference , Adobe Acrobat 9.53 Paper Capture Plug-in
- Full Text:
- Date Issued: 2013
- Authors: Schady, Stuart William
- Date: 2013 , 2013-04-29
- Subjects: Monetary policy -- South Africa , Inflation targeting -- South Africa , Gross domestic product -- South Africa , Bank loans -- South Africa , Money supply -- South Africa , Endogenous growth (Economics) , Domestic credit extension
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:928 , http://hdl.handle.net/10962/d1001454 , Monetary policy -- South Africa , Inflation targeting -- South Africa , Gross domestic product -- South Africa , Bank loans -- South Africa , Money supply -- South Africa , Endogenous growth (Economics)
- Description: This study is about whether the money supply in South Africa under a monetary policy regime of inflation‐targeting is exogenously or endogenously determined. The proposition of an exogenous money supply has been offered by monetarists, where the Central Bank determines the quantity of money supplied to the economy and this has a causal influence on income and credit extension. The endogenous money theory is a post‐Keynesian proposition whereby the money creation is determined by banks adjusting their responses to demands for credit‐money from economic agents. The data analysis is from 2000Q1 to 2010Q4 and entails the use of the variables monetary base (MB), domestic credit extension (DCE), M3, and gross national product (GDP). All variables are logged. The empirical tests conducted start with the Augmented Dickey‐Fuller unit root test to determine the variables order of integration. Johansen cointegration tests are done followed by Vector Error‐Correction Models (VECMs) and Granger causality tests to determine whether there is unidirectional or bidirectional causality between variables over the long and short‐run. Based on the results of the testing it was discovered that over the inflation‐targeting regime money supply in South Africa was endogenously determined. Furthermore, the data best supports the Accommodationist analysis of endogenous money as opposed to that of Structuralism and Liquidity Preference , Adobe Acrobat 9.53 Paper Capture Plug-in
- Full Text:
- Date Issued: 2013
Monitoring carbon stocks in the sub-tropical thicket biome using remote sensing and GIS techniques : the case of the Great Fish River Nature Reserve and its environs, Eastern Cape province, South Africa
- Authors: Nyamugama, Adolph
- Date: 2013
- Subjects: Fragmented landscapes -- South Africa -- Eastern Cape -- Remote sensing , Environmental degradation -- South Africa -- Eastern Cape , Remote sensing , Geographic information systems
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10672 , http://hdl.handle.net/10948/d1020303
- Description: The subtropical thicket biome in the Eastern Cape Province of South Africa has been heavily degraded and transformed due overutilization during the last century. The highly degraded and transformed areas exhibit a significant loss of above ground carbon stocks (AGC) and loss of SOC content. Information about land use /cover change and fragmentation dynamics is a prerequisite for measuring carbon stock changes. The main aim of this study is to assess the trends of land use/cover change, fragmentation dynamics, model the temporal changes of AGC stocks in the Great Fish River Nature Reserve and its environs from 1972 to 2010, quantify and map the spatial distribution of SOC concentrations in the partial subtropical thicket cover in the Great Fish River Nature Reserve and environs (communal rangelands). Multi-temporal analyses based on 1972 Landsat MSS, 1982 and 1992 Landsat TM, 2002 Landsat ETM and 2010 SPOT 5 High Resolution images were used for land use/cover change detection and fragmentation analysis. Object oriented post-classification comparison was applied for land use/cover change detection analysis. Fragmentation dynamics analysis was carried out by computing and analyzing landscape metrics in land use/cover classes. Landscape fragmentation analyses revealed that thicket vegetation has increasingly become fragmented, characterized by smaller less linked patches of intact thicket cover. Landscape metrics for intact thicket and degraded thicket classes reflected fragmentation, as illustrated by the increase in the Number of Patches (NP), Patch Density (PD), Landscape Shape Index (LSI), and a decrease in Mean Patch Size (MPS). The use of remote sensing techniques and landscape metrics was vital for the understanding of the dynamics of land use/cover change and fragmentation. Baseline land use/cover maps produced for 1972, 1982, 1992 2002 and 2010 and fragmentation analyses were then used for analyzing carbon stock changes in the study area. To model the temporal changes of AGC stocks in the Great Fish River Nature Reserve and its environs from 1972 to 2010, a method based on the integration of RS and GIS was employed for the estimation of AGC stocks in a time series. A non-linear regression model was developed using NDVI values generated from SPOT 5 HRG satellite imagery of 2010 as the independent variable and AGC stock estimates from field plots as the dependent variable. The regression model was used to estimate AGC stocks for the entire study area on the 2010 SPOT 5 HRG and also extrapolated to the 1972 Landsat MSS, 1982 and 1992 Landsat TM, and 2002 Landsat ETM. The AGC stocks for the period 1972 -1982, 1982-1992, 1992-200) and 2002-2010 were compared by means of change detection analysis. The comparison of AGC stocks was carried out at subtropical thicket class level. The results showed a decline of AGC stocks in all the classes from 1972 to 2010. Degraded and transformed thicket classes had the highest AGC stock losses. The decline of AGC stocks was attributed to thicket transformation and degradation which were caused by anthropogenic activities. To map and quantify SOC concentration in partial (fractional) thicket vegetation cover, the spectral reflectance of both thicket vegetation and bare-soils was measured in situ. Soil samples were collected from the sampling sites and transported to the laboratory for spectral reflectance and SOC measurements. Thicket vegetation and bare soil reflectance were measured using spectroscopy both in situ and under laboratory conditions. Their respective endmembers were extracted from ASTER imagery using the Pixel Purity Index (PPI). The endmembers were validated with in situ and laboratory thicket and bare-soil reflectance signatures. The spectral unmixing technique was applied to ASTER imagery to discriminate pure pixels of thicket vegetation and bare-soils; a residual spectral image was produced. The Residual Spectral Unmixing (RSU) procedure was applied to the residual spectral image to produce an RSU soil spectrum image. Partial Least Squares Regression (PSLR) model was developed using spectral signatures of a residual soil spectrum image as the independent variable and SOC concentration measured from soil samples as the dependent variable. The PSLR prediction model was used to predict SOC concentration on the RSU soil spectral image. The predicted SOC concentration was then validated with SOC concentration measured from the field plots. A Strong correlation (R2 = 0.82) was obtained between the predicted SOC concentration and the SOC concentration measured from field samples. The PSLR was then used to generate a map of SOC concentration for the Great Fish River Nature Reserve and its environs. Areas with very low SOC concentrations were found in the degraded communal villages, as opposed to the higher SOC values in the protected area. The results confirmed that RS techniques are key to estimating and mapping the spatial distribution of SOC concentration in partial subtropical thicket vegetation. Partial thicket vegetation has a huge influence on the soil spectra; it can influence the prediction of SOC concentration. The use of the RSU approach eliminates partial thicket vegetation cover from bare soil spectra. The residual soil spectrum image contains enough information for the mapping of SOC concentration. The technique has the potential to augment the applicability of airborne imaging spectroscopy for soil studies in the sub-tropical thicket biome and similar environments.
- Full Text:
- Date Issued: 2013
- Authors: Nyamugama, Adolph
- Date: 2013
- Subjects: Fragmented landscapes -- South Africa -- Eastern Cape -- Remote sensing , Environmental degradation -- South Africa -- Eastern Cape , Remote sensing , Geographic information systems
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:10672 , http://hdl.handle.net/10948/d1020303
- Description: The subtropical thicket biome in the Eastern Cape Province of South Africa has been heavily degraded and transformed due overutilization during the last century. The highly degraded and transformed areas exhibit a significant loss of above ground carbon stocks (AGC) and loss of SOC content. Information about land use /cover change and fragmentation dynamics is a prerequisite for measuring carbon stock changes. The main aim of this study is to assess the trends of land use/cover change, fragmentation dynamics, model the temporal changes of AGC stocks in the Great Fish River Nature Reserve and its environs from 1972 to 2010, quantify and map the spatial distribution of SOC concentrations in the partial subtropical thicket cover in the Great Fish River Nature Reserve and environs (communal rangelands). Multi-temporal analyses based on 1972 Landsat MSS, 1982 and 1992 Landsat TM, 2002 Landsat ETM and 2010 SPOT 5 High Resolution images were used for land use/cover change detection and fragmentation analysis. Object oriented post-classification comparison was applied for land use/cover change detection analysis. Fragmentation dynamics analysis was carried out by computing and analyzing landscape metrics in land use/cover classes. Landscape fragmentation analyses revealed that thicket vegetation has increasingly become fragmented, characterized by smaller less linked patches of intact thicket cover. Landscape metrics for intact thicket and degraded thicket classes reflected fragmentation, as illustrated by the increase in the Number of Patches (NP), Patch Density (PD), Landscape Shape Index (LSI), and a decrease in Mean Patch Size (MPS). The use of remote sensing techniques and landscape metrics was vital for the understanding of the dynamics of land use/cover change and fragmentation. Baseline land use/cover maps produced for 1972, 1982, 1992 2002 and 2010 and fragmentation analyses were then used for analyzing carbon stock changes in the study area. To model the temporal changes of AGC stocks in the Great Fish River Nature Reserve and its environs from 1972 to 2010, a method based on the integration of RS and GIS was employed for the estimation of AGC stocks in a time series. A non-linear regression model was developed using NDVI values generated from SPOT 5 HRG satellite imagery of 2010 as the independent variable and AGC stock estimates from field plots as the dependent variable. The regression model was used to estimate AGC stocks for the entire study area on the 2010 SPOT 5 HRG and also extrapolated to the 1972 Landsat MSS, 1982 and 1992 Landsat TM, and 2002 Landsat ETM. The AGC stocks for the period 1972 -1982, 1982-1992, 1992-200) and 2002-2010 were compared by means of change detection analysis. The comparison of AGC stocks was carried out at subtropical thicket class level. The results showed a decline of AGC stocks in all the classes from 1972 to 2010. Degraded and transformed thicket classes had the highest AGC stock losses. The decline of AGC stocks was attributed to thicket transformation and degradation which were caused by anthropogenic activities. To map and quantify SOC concentration in partial (fractional) thicket vegetation cover, the spectral reflectance of both thicket vegetation and bare-soils was measured in situ. Soil samples were collected from the sampling sites and transported to the laboratory for spectral reflectance and SOC measurements. Thicket vegetation and bare soil reflectance were measured using spectroscopy both in situ and under laboratory conditions. Their respective endmembers were extracted from ASTER imagery using the Pixel Purity Index (PPI). The endmembers were validated with in situ and laboratory thicket and bare-soil reflectance signatures. The spectral unmixing technique was applied to ASTER imagery to discriminate pure pixels of thicket vegetation and bare-soils; a residual spectral image was produced. The Residual Spectral Unmixing (RSU) procedure was applied to the residual spectral image to produce an RSU soil spectrum image. Partial Least Squares Regression (PSLR) model was developed using spectral signatures of a residual soil spectrum image as the independent variable and SOC concentration measured from soil samples as the dependent variable. The PSLR prediction model was used to predict SOC concentration on the RSU soil spectral image. The predicted SOC concentration was then validated with SOC concentration measured from the field plots. A Strong correlation (R2 = 0.82) was obtained between the predicted SOC concentration and the SOC concentration measured from field samples. The PSLR was then used to generate a map of SOC concentration for the Great Fish River Nature Reserve and its environs. Areas with very low SOC concentrations were found in the degraded communal villages, as opposed to the higher SOC values in the protected area. The results confirmed that RS techniques are key to estimating and mapping the spatial distribution of SOC concentration in partial subtropical thicket vegetation. Partial thicket vegetation has a huge influence on the soil spectra; it can influence the prediction of SOC concentration. The use of the RSU approach eliminates partial thicket vegetation cover from bare soil spectra. The residual soil spectrum image contains enough information for the mapping of SOC concentration. The technique has the potential to augment the applicability of airborne imaging spectroscopy for soil studies in the sub-tropical thicket biome and similar environments.
- Full Text:
- Date Issued: 2013
Morphological and genetic characterization of a South African Plutella xylostella granulovirus (plxy GV) isolate
- Abdulkadir, Fatima, Marsberg, Tamryn, Knox, Caroline M, Hill, Martin P, Moore, Sean D
- Authors: Abdulkadir, Fatima , Marsberg, Tamryn , Knox, Caroline M , Hill, Martin P , Moore, Sean D
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/406117 , vital:70240 , xlink:href="https://hdl.handle.net/10520/EJC132828"
- Description: Plutella xylostella (L.) (Lepidoptera: Plutellidae), also known as diamondback moth, is a destructive insect pest of cruciferous crops (Talekar and Shelton 1993; Shelton 2004). The pest occurs wherever its host plants are cultivated and the global annual cost of damage and control is estimated to be US$4-5 billion (Zalucki et al. 2012). The extensive use of synthetic pesticides for control combined with the high fecundity of P. xylostella has resulted in the pest developing resistance to nearly all classes of insecticides (Grzywacz et al. 2009). Moreover, these chemicals have negative environmental implications and may affect non-target species, some of which are natural enemies of the pest.
- Full Text:
- Date Issued: 2013
- Authors: Abdulkadir, Fatima , Marsberg, Tamryn , Knox, Caroline M , Hill, Martin P , Moore, Sean D
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/406117 , vital:70240 , xlink:href="https://hdl.handle.net/10520/EJC132828"
- Description: Plutella xylostella (L.) (Lepidoptera: Plutellidae), also known as diamondback moth, is a destructive insect pest of cruciferous crops (Talekar and Shelton 1993; Shelton 2004). The pest occurs wherever its host plants are cultivated and the global annual cost of damage and control is estimated to be US$4-5 billion (Zalucki et al. 2012). The extensive use of synthetic pesticides for control combined with the high fecundity of P. xylostella has resulted in the pest developing resistance to nearly all classes of insecticides (Grzywacz et al. 2009). Moreover, these chemicals have negative environmental implications and may affect non-target species, some of which are natural enemies of the pest.
- Full Text:
- Date Issued: 2013
Morphological and genetic variation in samango monkeys (Cercopithecus albogularis) in Southern Africa
- Authors: Makhasi, Ntuthuzelo
- Date: 2013
- Subjects: Morphology , Genetics
- Language: English
- Type: Thesis , Masters , MSc (Zoology)
- Identifier: vital:11793 , http://hdl.handle.net/10353/d1018580 , Morphology , Genetics
- Description: My aim was to resolve the taxonomy of the South African forms of Cercopithecus albogularis by exploring morphological and genetic variation in the two samango subspecies described for the region: C. a. erythrarchus and C. a. labiatus. In addition, I estimated their geographic distributions and habitat requirements from the provenance data I collected during my study of museum specimens. My analysis has shown clear morphological differences between C. a. labiatus and C. a. erythrarchus. The two subspecies differ in pelage coloration, but also in cranial shape and tail lengths. Furthermore, C. a. labiatus is slightly smaller, shorter-tailed and stocky compared with the long-tailed slender northern forms of C. albogularis, which may be adaptations to cold environments like montane forest. The northern C. albogularis subspecies are distinguishable from the southern taxa with a high degree of reliability (98.5%), suggesting that C. albogularis consists of more than one species. The most appropriate name for the southern species is C. labiatus. My genetic study did not detect variation among the 10 animals sampled in Hogsback; while it clustered the different taxa, it could not resolve relationships between them, with the exception of the outgroup. The lack of resolution of the deeper nodes could be a result of the fact that our sequence was very short (274 bp). The mt 12S rRNA gene was not an ideal gene for this study, which should have involved a less conserved section of the mtDNA molecule, like the rapidly evolving D-loop. More genetic work is clearly needed to resolve the phylogenetic relationships within the C. mitis supergroup. However, preliminary genetic data indicate that the southern samangos are distinct from the C. mitis of West Africa, while my morphometric study suggests they may also be distinct from C. albogularis in East Africa. Molecular and karyological studies comparing the genomes of Hogsback “C. a. labiatus” with the neighbouring “C. a. erythrarchus” and Zanzibar C. albogularis would be extremely enlightening.
- Full Text:
- Date Issued: 2013
- Authors: Makhasi, Ntuthuzelo
- Date: 2013
- Subjects: Morphology , Genetics
- Language: English
- Type: Thesis , Masters , MSc (Zoology)
- Identifier: vital:11793 , http://hdl.handle.net/10353/d1018580 , Morphology , Genetics
- Description: My aim was to resolve the taxonomy of the South African forms of Cercopithecus albogularis by exploring morphological and genetic variation in the two samango subspecies described for the region: C. a. erythrarchus and C. a. labiatus. In addition, I estimated their geographic distributions and habitat requirements from the provenance data I collected during my study of museum specimens. My analysis has shown clear morphological differences between C. a. labiatus and C. a. erythrarchus. The two subspecies differ in pelage coloration, but also in cranial shape and tail lengths. Furthermore, C. a. labiatus is slightly smaller, shorter-tailed and stocky compared with the long-tailed slender northern forms of C. albogularis, which may be adaptations to cold environments like montane forest. The northern C. albogularis subspecies are distinguishable from the southern taxa with a high degree of reliability (98.5%), suggesting that C. albogularis consists of more than one species. The most appropriate name for the southern species is C. labiatus. My genetic study did not detect variation among the 10 animals sampled in Hogsback; while it clustered the different taxa, it could not resolve relationships between them, with the exception of the outgroup. The lack of resolution of the deeper nodes could be a result of the fact that our sequence was very short (274 bp). The mt 12S rRNA gene was not an ideal gene for this study, which should have involved a less conserved section of the mtDNA molecule, like the rapidly evolving D-loop. More genetic work is clearly needed to resolve the phylogenetic relationships within the C. mitis supergroup. However, preliminary genetic data indicate that the southern samangos are distinct from the C. mitis of West Africa, while my morphometric study suggests they may also be distinct from C. albogularis in East Africa. Molecular and karyological studies comparing the genomes of Hogsback “C. a. labiatus” with the neighbouring “C. a. erythrarchus” and Zanzibar C. albogularis would be extremely enlightening.
- Full Text:
- Date Issued: 2013
Morphology, patterns and processes in the Oyster Bay headland bypass dunefield, South Africa
- Authors: McConnachie, Lauren Bernyse
- Date: 2013
- Subjects: Sand dune conservation -- South Africa -- Eastern Cape Sand dune ecology -- South Africa -- Eastern Cape Coastal ecology -- South Africa -- Eastern Cape Sedimentation and deposition -- South Africa -- Eastern Cape Beach erosion -- South Africa -- Eastern Cape Coastal zone management -- South Africa -- Eastern Cape Shore protection -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4804 , http://hdl.handle.net/10962/d1001904
- Description: Studies of the dunefield systems crossing the Cape St. Francis headland in the Eastern Cape have focused on the role that wind plays in sediment transfer in coastal dunefield systems, with limited consideration of the role of water. The aim of this study was to improve understanding of the morphology, processes and patterns within the Oyster Bay HBD system, focussing particularly on surface water and groundwater interactions and the role of surface water in sediment transfer across the dunefield system. An extensive field survey was conducted, to collect related data, complimented by spatial and temporal analysis of the study area using GIS. The key findings from this research were the apparent differences between the western and eastern regions of the dunefield with regard to specific drivers and the respective processes and responses. Wind is the major driver of change up to and across the crest of the dunefield. In the eastern region water (ground water, surface water and the Sand River System) is the primary agent of sediment flux through processes of aggregation and slumping as well as episodic events including debris flows. This study has highlighted a need for further quantitative studies that investigate the movement of sediment through dunefield systems such as this (where water is at or near the land surface). The paradigm that sediment flux is entirely due to wind is almost certainly simplistic, and deeper understanding of these systems is needed , Maiden name: Elkington, Lauren
- Full Text:
- Date Issued: 2013
- Authors: McConnachie, Lauren Bernyse
- Date: 2013
- Subjects: Sand dune conservation -- South Africa -- Eastern Cape Sand dune ecology -- South Africa -- Eastern Cape Coastal ecology -- South Africa -- Eastern Cape Sedimentation and deposition -- South Africa -- Eastern Cape Beach erosion -- South Africa -- Eastern Cape Coastal zone management -- South Africa -- Eastern Cape Shore protection -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4804 , http://hdl.handle.net/10962/d1001904
- Description: Studies of the dunefield systems crossing the Cape St. Francis headland in the Eastern Cape have focused on the role that wind plays in sediment transfer in coastal dunefield systems, with limited consideration of the role of water. The aim of this study was to improve understanding of the morphology, processes and patterns within the Oyster Bay HBD system, focussing particularly on surface water and groundwater interactions and the role of surface water in sediment transfer across the dunefield system. An extensive field survey was conducted, to collect related data, complimented by spatial and temporal analysis of the study area using GIS. The key findings from this research were the apparent differences between the western and eastern regions of the dunefield with regard to specific drivers and the respective processes and responses. Wind is the major driver of change up to and across the crest of the dunefield. In the eastern region water (ground water, surface water and the Sand River System) is the primary agent of sediment flux through processes of aggregation and slumping as well as episodic events including debris flows. This study has highlighted a need for further quantitative studies that investigate the movement of sediment through dunefield systems such as this (where water is at or near the land surface). The paradigm that sediment flux is entirely due to wind is almost certainly simplistic, and deeper understanding of these systems is needed , Maiden name: Elkington, Lauren
- Full Text:
- Date Issued: 2013
Morphology, patterns and processes in the Oyster Bay headland bypass dunefield, South Africa
- Authors: Elkington, Lauren
- Date: 2013
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:20984 , http://hdl.handle.net/10962/5879
- Description: Studies of the dunefield systems crossing the Cape St. Francis headland in the Eastern Cape have focused on the role that wind plays in sediment transfer in coastal dunefield systems, with limited consideration of the role of water. The aim of this study was to improve understanding of the morphology, processes and patterns within the Oyster Bay HBD system, focussing particularly on surface water and groundwater interactions and the role of surface water in sediment transfer across the dunefield system. An extensive field survey was conducted, to collect related data, complimented by spatial and temporal analysis of the study area using GIS. The key findings from this research were the apparent differences between the western and eastern regions of the dunefield with regard to specific drivers and the respective processes and responses. Wind is the major driver of change up to and across the crest of the dunefield. In the eastern region water (ground water, surface water and the Sand River System) is the primary agent of sediment flux through processes of aggregation and slumping as well as episodic events including debris flows. This study has highlighted a need for further quantitative studies that investigate the movement of sediment through dunefield systems such as this (where water is at or near the land surface). The paradigm that sediment flux is entirely due to wind is almost certainly simplistic, and deeper understanding of these systems is needed.
- Full Text:
- Date Issued: 2013
- Authors: Elkington, Lauren
- Date: 2013
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:20984 , http://hdl.handle.net/10962/5879
- Description: Studies of the dunefield systems crossing the Cape St. Francis headland in the Eastern Cape have focused on the role that wind plays in sediment transfer in coastal dunefield systems, with limited consideration of the role of water. The aim of this study was to improve understanding of the morphology, processes and patterns within the Oyster Bay HBD system, focussing particularly on surface water and groundwater interactions and the role of surface water in sediment transfer across the dunefield system. An extensive field survey was conducted, to collect related data, complimented by spatial and temporal analysis of the study area using GIS. The key findings from this research were the apparent differences between the western and eastern regions of the dunefield with regard to specific drivers and the respective processes and responses. Wind is the major driver of change up to and across the crest of the dunefield. In the eastern region water (ground water, surface water and the Sand River System) is the primary agent of sediment flux through processes of aggregation and slumping as well as episodic events including debris flows. This study has highlighted a need for further quantitative studies that investigate the movement of sediment through dunefield systems such as this (where water is at or near the land surface). The paradigm that sediment flux is entirely due to wind is almost certainly simplistic, and deeper understanding of these systems is needed.
- Full Text:
- Date Issued: 2013
Movement patterns and genetic stock delineation of an endemic South African sparid, the Poenskop, Cymatoceps nasutus (Castelnau, 1861)
- Authors: Murray, Taryn Sara
- Date: 2013
- Subjects: Fish stock assessment -- South Africa , Sparidae -- South Africa , Fishes -- Reproduction -- South Africa , Fishing -- South Africa , Fish populations -- South Africa , Fishes -- Behavior -- South Africa , Marine fishes -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5178 , http://hdl.handle.net/10962/d1001514 , Fish stock assessment -- South Africa , Sparidae -- South Africa , Fishes -- Reproduction -- South Africa , Fishing -- South Africa , Fish populations -- South Africa , Fishes -- Behavior -- South Africa , Marine fishes -- South Africa
- Description: Poenskop Cymatoceps nasutus (Pisces: Sparidae), an endemic South African sparid, is an important angling species being predominantly targeted by the recreational shore and skiboat sector. This species is slow-growing, long-lived, late-maturing and sex-changing, making poenskop acutely sensitive to over-exploitation. Despite interventions, such as the imposition of size and bag limits (currently 50 cm TL and one per licensed fisher per day) by authorities, catch-per-unit-effort trends reflect a severe and consistent stock decline over the last two decades. Poenskop has been identified as a priority species for research and conservation. Although the biology and population dynamics of this species have been well-documented, little is known about the movement behaviour of poenskop. Furthermore, there is a complete lack of information on its genetic stock structure. This thesis aimed to address the current knowledge gaps concerning movement behaviour and genetic stock structure of poenskop, making use of a range of methods and drawing on available information, including available fishery records as well as published and unpublished survey and research data, and data from long-term monitoring programmes. Analysis of available catch data (published and unpublished) revealed a decline in the number of poenskop caught as well as size of fish taken over the last two decades, ultimately reflecting the collapse of the stock (estimated to be at 20% of their pristine level). Improved catch-per-unit-effort data from the Tsitsikamma National Park Marine Protected Area (MPA), and larger poenskop being caught in the no-take areas than adjacent exploited areas of the Pondoland MPA confirmed that MPAs can be effective for the protection and management of poenskop. The current MPA network in South Africa is already wellestablished, and encompasses considerable reef areas, being preferable for poenskop habitation. Conventional dart tagging and recapture information from three ongoing, long-term fishtagging projects, conducted throughout the poenskop’s distribution, indicated high levels of residency at all life-history stages. Coastal region, seasonality and time at recapture did not appear to have a significant effect on the level of movement or distance moved. However, on examining the relationship among coastal movements and fish size and ages, larger and older fish (adults) moved greater distances, with juveniles and sub-adults showing high degrees of residency. An estimation of home-range size indicated smaller poenskop to hold smaller home-ranges, while larger poenskop hold larger home-ranges. Large easterly displacements of a number of adult poenskop is in accordance with previous findings that this species may undertake a unidirectional migration up the coastline of South Africa where they possibly settle in Transkei waters for the remainder of their lives. This high level of residency makes poenskop vulnerable to localised depletion, although they can be effectively protected by suitable MPAs. Despite considerable tagging effort along the South African coastline (2 704 poenskop tagged with 189 recaptures, between 1984 and 2010), there remains limited information on the connectivity of different regions along the South African coastline. This was addressed using mitochondrial DNA sequencing. The mitochondrial DNA control region was used due to its high substitution rate, haploid nature, maternal inheritance and absence of recombination. The mtDNA sequencing showed no evidence of major geographic barriers to gene flow in this species. Samples collected throughout the core distribution of poenskop showed high genetic diversity (h = 0.88, π = 0.01), low genetic differentiation among regions, no spatial structure (ɸST = 0.012, p = 0.208) and no evidence of isolation by distance. The collapsed stock status of poenskop as well as the fact that it is being actively targeted by recreational and commercial fishers suggests that this species requires improved management, with consideration given to its life-history style, residency and poor conservation status. Management recommendations for poenskop, combined with increasing South Africa’s existing MPA network, include the possibility of setting up a closed season (during known spawning periods) as well as the decommercialisation of this species. The techniques used and developed in this study can also be adopted for other overexploited linefish species.
- Full Text:
- Date Issued: 2013
- Authors: Murray, Taryn Sara
- Date: 2013
- Subjects: Fish stock assessment -- South Africa , Sparidae -- South Africa , Fishes -- Reproduction -- South Africa , Fishing -- South Africa , Fish populations -- South Africa , Fishes -- Behavior -- South Africa , Marine fishes -- South Africa
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5178 , http://hdl.handle.net/10962/d1001514 , Fish stock assessment -- South Africa , Sparidae -- South Africa , Fishes -- Reproduction -- South Africa , Fishing -- South Africa , Fish populations -- South Africa , Fishes -- Behavior -- South Africa , Marine fishes -- South Africa
- Description: Poenskop Cymatoceps nasutus (Pisces: Sparidae), an endemic South African sparid, is an important angling species being predominantly targeted by the recreational shore and skiboat sector. This species is slow-growing, long-lived, late-maturing and sex-changing, making poenskop acutely sensitive to over-exploitation. Despite interventions, such as the imposition of size and bag limits (currently 50 cm TL and one per licensed fisher per day) by authorities, catch-per-unit-effort trends reflect a severe and consistent stock decline over the last two decades. Poenskop has been identified as a priority species for research and conservation. Although the biology and population dynamics of this species have been well-documented, little is known about the movement behaviour of poenskop. Furthermore, there is a complete lack of information on its genetic stock structure. This thesis aimed to address the current knowledge gaps concerning movement behaviour and genetic stock structure of poenskop, making use of a range of methods and drawing on available information, including available fishery records as well as published and unpublished survey and research data, and data from long-term monitoring programmes. Analysis of available catch data (published and unpublished) revealed a decline in the number of poenskop caught as well as size of fish taken over the last two decades, ultimately reflecting the collapse of the stock (estimated to be at 20% of their pristine level). Improved catch-per-unit-effort data from the Tsitsikamma National Park Marine Protected Area (MPA), and larger poenskop being caught in the no-take areas than adjacent exploited areas of the Pondoland MPA confirmed that MPAs can be effective for the protection and management of poenskop. The current MPA network in South Africa is already wellestablished, and encompasses considerable reef areas, being preferable for poenskop habitation. Conventional dart tagging and recapture information from three ongoing, long-term fishtagging projects, conducted throughout the poenskop’s distribution, indicated high levels of residency at all life-history stages. Coastal region, seasonality and time at recapture did not appear to have a significant effect on the level of movement or distance moved. However, on examining the relationship among coastal movements and fish size and ages, larger and older fish (adults) moved greater distances, with juveniles and sub-adults showing high degrees of residency. An estimation of home-range size indicated smaller poenskop to hold smaller home-ranges, while larger poenskop hold larger home-ranges. Large easterly displacements of a number of adult poenskop is in accordance with previous findings that this species may undertake a unidirectional migration up the coastline of South Africa where they possibly settle in Transkei waters for the remainder of their lives. This high level of residency makes poenskop vulnerable to localised depletion, although they can be effectively protected by suitable MPAs. Despite considerable tagging effort along the South African coastline (2 704 poenskop tagged with 189 recaptures, between 1984 and 2010), there remains limited information on the connectivity of different regions along the South African coastline. This was addressed using mitochondrial DNA sequencing. The mitochondrial DNA control region was used due to its high substitution rate, haploid nature, maternal inheritance and absence of recombination. The mtDNA sequencing showed no evidence of major geographic barriers to gene flow in this species. Samples collected throughout the core distribution of poenskop showed high genetic diversity (h = 0.88, π = 0.01), low genetic differentiation among regions, no spatial structure (ɸST = 0.012, p = 0.208) and no evidence of isolation by distance. The collapsed stock status of poenskop as well as the fact that it is being actively targeted by recreational and commercial fishers suggests that this species requires improved management, with consideration given to its life-history style, residency and poor conservation status. Management recommendations for poenskop, combined with increasing South Africa’s existing MPA network, include the possibility of setting up a closed season (during known spawning periods) as well as the decommercialisation of this species. The techniques used and developed in this study can also be adopted for other overexploited linefish species.
- Full Text:
- Date Issued: 2013
Movement patterns and habitat selection of invasive African sharptooth catfish
- Kadye, Wilbert T, Booth, Anthony J
- Authors: Kadye, Wilbert T , Booth, Anthony J
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/125276 , vital:35767 , http://dx.doi.10.1111/j.1469-7998.2012.00960.x
- Description: Information on the movement behaviour and habitat use by non-native invasive African catfish Clarias gariepinus is crucial in understanding and possibly mitigating its potential impacts. The aim of this study was to examine catfish movement and habitat selection within an invaded impoundment in the Eastern Cape, South Africa. Acoustic telemetry data for 10 tagged catfish were analyzed to identify spatial patterns in home ranges and seasonal changes in habitat associations. Long-distance movements were observed for most catfish from common central release point, whereas short-distance movements defined their home ranges and utilization distributions that were categorized as localized within single or multiple habitats. Habitat selection was non-random with most catfish utilizing the shallow river mouth and upper section of the reservoir that were dominated by a rocky substratum interspersed with submerged trees. These localities were likely to be preferred for spawning and/or feeding. Utilization of these habitats by catfish is likely to be associated with probable impact due to predation and interference competition for feeding and breeding grounds with other species. Although most catfish maintained their home ranges throughout the study, seasonal shifts in habitat use, which was reflected by the utilization of deep and silt-dominated habitats, were also observed for some catfish. Non-random habitat use and homing behaviour within single and multiple habitats by non-native sharptooth catfish suggests that its impact within the invaded habitats may be associated with particular habitats both at broad spatial and temporal scales. Protection of habitats from catfish invasion should be considered as a management option to conserve native biota.
- Full Text:
- Date Issued: 2013
- Authors: Kadye, Wilbert T , Booth, Anthony J
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/125276 , vital:35767 , http://dx.doi.10.1111/j.1469-7998.2012.00960.x
- Description: Information on the movement behaviour and habitat use by non-native invasive African catfish Clarias gariepinus is crucial in understanding and possibly mitigating its potential impacts. The aim of this study was to examine catfish movement and habitat selection within an invaded impoundment in the Eastern Cape, South Africa. Acoustic telemetry data for 10 tagged catfish were analyzed to identify spatial patterns in home ranges and seasonal changes in habitat associations. Long-distance movements were observed for most catfish from common central release point, whereas short-distance movements defined their home ranges and utilization distributions that were categorized as localized within single or multiple habitats. Habitat selection was non-random with most catfish utilizing the shallow river mouth and upper section of the reservoir that were dominated by a rocky substratum interspersed with submerged trees. These localities were likely to be preferred for spawning and/or feeding. Utilization of these habitats by catfish is likely to be associated with probable impact due to predation and interference competition for feeding and breeding grounds with other species. Although most catfish maintained their home ranges throughout the study, seasonal shifts in habitat use, which was reflected by the utilization of deep and silt-dominated habitats, were also observed for some catfish. Non-random habitat use and homing behaviour within single and multiple habitats by non-native sharptooth catfish suggests that its impact within the invaded habitats may be associated with particular habitats both at broad spatial and temporal scales. Protection of habitats from catfish invasion should be considered as a management option to conserve native biota.
- Full Text:
- Date Issued: 2013
Multiple representations and cognitive load: words, arrows, and colours when solving algebraic problems
- Authors: Brey, Amina
- Date: 2013
- Subjects: Algebraic logic , Mathematical analysis , Mathematics -- Study and teaching
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:9580 , http://hdl.handle.net/10948/d1020392
- Description: This study investigates the possible effects that access to selected multiple representations (words, arrows and colours) have in terms of cognitive load and learner achievement when presented with algebraic problems at grade nine level. The presentation of multiple representations (the intervention) was intended to decrease extraneous cognitive load, manage the intrinsic cognitive load (algebraic problems) and optimise germane cognition (schema acquisition and automation). An explanatory sequential mixed-method design was employed with six hundred and seventy three learners in four secondary schools. Quantitative data were generated via pre-, intervention and post-tests/questionnaires, while qualitative data were obtained from open-ended questions in the pre-, intervention, and post-tests/questionnaires, eight learner focus group interviews (n = 32), and four semi-structured, open-ended teacher interviews. Statistically and practically significant improvement in mean test scores from the pre- to intervention test scores in all schools was noted. No statistically and practically significant improvement was noted in further post-tests except for post-test 2 which employed more challenging problems (statistically significant decrease with a small practical effect). Learners expressed their preference for arrows, followed by colours and then words as effective representations. Teacher generated qualitative data suggests that they realise the importance of using multiple representations as an instructional strategy and implicitly understand the notion of cognitive load. The findings, when considered in the light of literature on cognitive load, suggest that a reduction in extraneous cognitive load by using a more effective instructional design (multiple representations) frees working memory capacity which can then be devoted to the intrinsic cognitive load (algebraic problems) and thereby increase germane cognition (schema acquisition and automation).
- Full Text:
- Date Issued: 2013
- Authors: Brey, Amina
- Date: 2013
- Subjects: Algebraic logic , Mathematical analysis , Mathematics -- Study and teaching
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:9580 , http://hdl.handle.net/10948/d1020392
- Description: This study investigates the possible effects that access to selected multiple representations (words, arrows and colours) have in terms of cognitive load and learner achievement when presented with algebraic problems at grade nine level. The presentation of multiple representations (the intervention) was intended to decrease extraneous cognitive load, manage the intrinsic cognitive load (algebraic problems) and optimise germane cognition (schema acquisition and automation). An explanatory sequential mixed-method design was employed with six hundred and seventy three learners in four secondary schools. Quantitative data were generated via pre-, intervention and post-tests/questionnaires, while qualitative data were obtained from open-ended questions in the pre-, intervention, and post-tests/questionnaires, eight learner focus group interviews (n = 32), and four semi-structured, open-ended teacher interviews. Statistically and practically significant improvement in mean test scores from the pre- to intervention test scores in all schools was noted. No statistically and practically significant improvement was noted in further post-tests except for post-test 2 which employed more challenging problems (statistically significant decrease with a small practical effect). Learners expressed their preference for arrows, followed by colours and then words as effective representations. Teacher generated qualitative data suggests that they realise the importance of using multiple representations as an instructional strategy and implicitly understand the notion of cognitive load. The findings, when considered in the light of literature on cognitive load, suggest that a reduction in extraneous cognitive load by using a more effective instructional design (multiple representations) frees working memory capacity which can then be devoted to the intrinsic cognitive load (algebraic problems) and thereby increase germane cognition (schema acquisition and automation).
- Full Text:
- Date Issued: 2013
Nanoconjugates of CdTe@ ZnS quantum dots with cobalt tetraamino-phthalocyanine
- Adegoke, Oluwasesan, Antunes, Edith M, Nyokong, Tebello
- Authors: Adegoke, Oluwasesan , Antunes, Edith M , Nyokong, Tebello
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/242049 , vital:50996 , xlink:href="https://doi.org/10.1016/j.jphotochem.2013.02.010"
- Description: The covalent linking of thiol-capped CdTe@ZnS QDs with cobalt tetraamino-phthalocyanine (CoTAPc) has been successfully carried out. Several techniques such as time-resolved fluorescence measurements, thermal gravimetric analysis,transmissionelectronmicroscopy andspectrophotometric techniques were employed to characterize the nanoconjugates. Covalent binding of the QDs with CoTAPc resulted in the fluorescence quenching of the former. In the presence of varying concentrations of superoxide anion (O2 •−), the fluorescence of the QDs in the conjugate was gradually enhanced and the detection limits obtained were 2.1 and 2.4 nM for the smaller and larger QDs, respectively. Based on the excellent selectivity displayed by the nanoconjugates towards O2 •− over other biologically active species, a potential nanosensor was developed.
- Full Text:
- Date Issued: 2013
- Authors: Adegoke, Oluwasesan , Antunes, Edith M , Nyokong, Tebello
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/242049 , vital:50996 , xlink:href="https://doi.org/10.1016/j.jphotochem.2013.02.010"
- Description: The covalent linking of thiol-capped CdTe@ZnS QDs with cobalt tetraamino-phthalocyanine (CoTAPc) has been successfully carried out. Several techniques such as time-resolved fluorescence measurements, thermal gravimetric analysis,transmissionelectronmicroscopy andspectrophotometric techniques were employed to characterize the nanoconjugates. Covalent binding of the QDs with CoTAPc resulted in the fluorescence quenching of the former. In the presence of varying concentrations of superoxide anion (O2 •−), the fluorescence of the QDs in the conjugate was gradually enhanced and the detection limits obtained were 2.1 and 2.4 nM for the smaller and larger QDs, respectively. Based on the excellent selectivity displayed by the nanoconjugates towards O2 •− over other biologically active species, a potential nanosensor was developed.
- Full Text:
- Date Issued: 2013
Nanostructured β-cyclodextrin-hyperbranched polyethyleneimine (β-CD-HPEI) embedded in polysulfone membrane for the removal of humic acid from water
- Malinga, S P, Arotiba, O A, Mapolie, S F, Diallo, M S, Mamba, Bhekie B, Krause, Rui W M
- Authors: Malinga, S P , Arotiba, O A , Mapolie, S F , Diallo, M S , Mamba, Bhekie B , Krause, Rui W M
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/125119 , vital:35732 , https://doi.org/10.1080/01496395.2013.809108
- Description: The synthesis of a new β-cyclodextrin-hyperbranched polyethyleneimine (β-CD-HPEI)/polysulfone (PSf) membranes via interfacial polymerization of trimesoyl chloride and β-CD-HPEI is described in this paper. The membranes were characterized by atomic force microscopy (AFM), high resolution scanning electron microscopy (HR-SEM) and contact-angle measurements. Water permeability and rejection data were obtained using a cross-flow filtration system at 0.69 MPa. The membranes were hydrophilic (25° to 63°), showed high humic acid rejection (>amp;80%), and maintained a constant flux throughout the filtration. The modified membranes were rougher than the pristine PSf membranes but they exhibited better antifouling properties due to the hydrophilic surface which acted as a barrier against humic acid deposition. The modification of PSf with β-CD-HPEI resulted in enhanced hydrophilicity and water permeability while still maintaining high humic acid rejection. Supplemental materials are available for this article. Go to the publisher's online edition of Separation Science & Technology to view the supplemental file.
- Full Text:
- Date Issued: 2013
- Authors: Malinga, S P , Arotiba, O A , Mapolie, S F , Diallo, M S , Mamba, Bhekie B , Krause, Rui W M
- Date: 2013
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/125119 , vital:35732 , https://doi.org/10.1080/01496395.2013.809108
- Description: The synthesis of a new β-cyclodextrin-hyperbranched polyethyleneimine (β-CD-HPEI)/polysulfone (PSf) membranes via interfacial polymerization of trimesoyl chloride and β-CD-HPEI is described in this paper. The membranes were characterized by atomic force microscopy (AFM), high resolution scanning electron microscopy (HR-SEM) and contact-angle measurements. Water permeability and rejection data were obtained using a cross-flow filtration system at 0.69 MPa. The membranes were hydrophilic (25° to 63°), showed high humic acid rejection (>amp;80%), and maintained a constant flux throughout the filtration. The modified membranes were rougher than the pristine PSf membranes but they exhibited better antifouling properties due to the hydrophilic surface which acted as a barrier against humic acid deposition. The modification of PSf with β-CD-HPEI resulted in enhanced hydrophilicity and water permeability while still maintaining high humic acid rejection. Supplemental materials are available for this article. Go to the publisher's online edition of Separation Science & Technology to view the supplemental file.
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- Date Issued: 2013
Navi Pillay: Realising Human Rights for All
- Authors: Naidu, Samantha
- Date: 2013
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/157960 , vital:40134 , ISBN 978-1906413453
- Description: Navi Pillay has become one of the world's leading advocates in the field of human rights. This new biography tells the story of her humble beginnings in an Indian family in apartheid South Africa, her struggle to gain an education, and her eventual success by becoming the first black woman in the nation to set up a law practice. She defended many anti-apartheid activists and fought for the rights of the mostly political prisoners of Robben Island. In 1995 Nelson Mandela nominated Pillay as the first black female judge on South Africa's Supreme Court. This is the story of how she overcame enormous obstacles to achieve her dream of human rights.
- Full Text:
- Date Issued: 2013
- Authors: Naidu, Samantha
- Date: 2013
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/157960 , vital:40134 , ISBN 978-1906413453
- Description: Navi Pillay has become one of the world's leading advocates in the field of human rights. This new biography tells the story of her humble beginnings in an Indian family in apartheid South Africa, her struggle to gain an education, and her eventual success by becoming the first black woman in the nation to set up a law practice. She defended many anti-apartheid activists and fought for the rights of the mostly political prisoners of Robben Island. In 1995 Nelson Mandela nominated Pillay as the first black female judge on South Africa's Supreme Court. This is the story of how she overcame enormous obstacles to achieve her dream of human rights.
- Full Text:
- Date Issued: 2013
Needed: a broader view of accountability: regulation, ethics, accountability
- Authors: Friedman, Steven
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/454822 , vital:75379 , https://hdl.handle.net/10520/EJC141586
- Description: Political journalists and judges might be offended by claims that they have much in common. But the commonality exists nonetheless. Both media and courts insist that their independence is crucial to a democrat-ic society. Both see themselves as a vital check on the power of gov-ernment. And both therefore resist calls that they should be accounta-ble to society. Criticism of judges and political journalists, even when accompanied by no demands that they be forced to do anything differ-ently, are often rejected as an assault on the independence of both.
- Full Text:
- Date Issued: 2013
- Authors: Friedman, Steven
- Date: 2013
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/454822 , vital:75379 , https://hdl.handle.net/10520/EJC141586
- Description: Political journalists and judges might be offended by claims that they have much in common. But the commonality exists nonetheless. Both media and courts insist that their independence is crucial to a democrat-ic society. Both see themselves as a vital check on the power of gov-ernment. And both therefore resist calls that they should be accounta-ble to society. Criticism of judges and political journalists, even when accompanied by no demands that they be forced to do anything differ-ently, are often rejected as an assault on the independence of both.
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- Date Issued: 2013
Negotiating identity and belonging: perspectives of children living in a disadvantaged community in the Eastern Cape province
- Authors: Ridley, Tamerin Amy
- Date: 2013
- Subjects: Group identity , Self-esteem
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/24813 , vital:63598
- Description: Developing an identity with self-esteem and a sense of self-worth is a child’s fundamental right (Vandenbroek, 2001). To encourage identity formation children need to ask and answer questions such as: ‘Who am I?’, ‘Where do I belong?’ and ‘Is it ok to be who I am?’ A child’s identity is shaped largely by his/her experiences with regards to relationships and belonging within communities and familial structures. However, South Africa faces a host of problems, including poverty, violence, HIV/AIDS, all of which contribute to the breakdown of these familial and community structures. Utilising a participatory action framework, this research aims to provide insight into how children living in a disadvantaged community negotiate identity and belonging. This insight into children’s perceptions of identity and belonging is useful for identifying resources within the community which promote a positive sense of identity and belonging, and also to identify areas where support and intervention are required. , Thesis (MSoc) -- Faculty of Social Sciences and Humanities, 2013
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- Date Issued: 2013
- Authors: Ridley, Tamerin Amy
- Date: 2013
- Subjects: Group identity , Self-esteem
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10353/24813 , vital:63598
- Description: Developing an identity with self-esteem and a sense of self-worth is a child’s fundamental right (Vandenbroek, 2001). To encourage identity formation children need to ask and answer questions such as: ‘Who am I?’, ‘Where do I belong?’ and ‘Is it ok to be who I am?’ A child’s identity is shaped largely by his/her experiences with regards to relationships and belonging within communities and familial structures. However, South Africa faces a host of problems, including poverty, violence, HIV/AIDS, all of which contribute to the breakdown of these familial and community structures. Utilising a participatory action framework, this research aims to provide insight into how children living in a disadvantaged community negotiate identity and belonging. This insight into children’s perceptions of identity and belonging is useful for identifying resources within the community which promote a positive sense of identity and belonging, and also to identify areas where support and intervention are required. , Thesis (MSoc) -- Faculty of Social Sciences and Humanities, 2013
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- Date Issued: 2013
Negotiating identity and belonging: perspectives of children living in a disadvantaged community in the Eastern Cape Province
- Authors: Ridley, Tamerin Amy
- Date: 2013
- Subjects: Group identity -- South Africa -- Eastern Cape , Children's rights -- South Africa -- Eastern Cape , Broken homes -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , M Soc Sc (C Psy)
- Identifier: vital:11860 , http://hdl.handle.net/10353/d1019871 , Group identity -- South Africa -- Eastern Cape , Children's rights -- South Africa -- Eastern Cape , Broken homes -- South Africa -- Eastern Cape
- Description: Developing an identity with self-esteem and a sense of self-worth is a child’s fundamental right (Vandenbroek, 2001). To encourage identity formation children need to ask and answer questions such as: ‘Who am I?’, ‘Where do I belong?’ and ‘Is it ok to be who I am?’ A child’s identity is shaped largely by his/her experiences with regards to relationships and belonging within communities and familial structures. However, South Africa faces a host of problems, including poverty, violence, HIV/AIDS, all of which contribute to the breakdown of these familial and community structures. Utilising a participatory action framework, this research aims to provide insight into how children living in a disadvantaged community negotiate identity and belonging. This insight into children’s perceptions of identity and belonging is useful for identifying resources within the community which promote a positive sense of identity and belonging, and also to identify areas where support and intervention are required.
- Full Text:
- Date Issued: 2013
- Authors: Ridley, Tamerin Amy
- Date: 2013
- Subjects: Group identity -- South Africa -- Eastern Cape , Children's rights -- South Africa -- Eastern Cape , Broken homes -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , M Soc Sc (C Psy)
- Identifier: vital:11860 , http://hdl.handle.net/10353/d1019871 , Group identity -- South Africa -- Eastern Cape , Children's rights -- South Africa -- Eastern Cape , Broken homes -- South Africa -- Eastern Cape
- Description: Developing an identity with self-esteem and a sense of self-worth is a child’s fundamental right (Vandenbroek, 2001). To encourage identity formation children need to ask and answer questions such as: ‘Who am I?’, ‘Where do I belong?’ and ‘Is it ok to be who I am?’ A child’s identity is shaped largely by his/her experiences with regards to relationships and belonging within communities and familial structures. However, South Africa faces a host of problems, including poverty, violence, HIV/AIDS, all of which contribute to the breakdown of these familial and community structures. Utilising a participatory action framework, this research aims to provide insight into how children living in a disadvantaged community negotiate identity and belonging. This insight into children’s perceptions of identity and belonging is useful for identifying resources within the community which promote a positive sense of identity and belonging, and also to identify areas where support and intervention are required.
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- Date Issued: 2013
Nelson Mandela Metropolitan University students' perceptions of celebrity endorsement and product placement within music videos
- Authors: Haldane, Jayne
- Date: 2013
- Subjects: Product placement in mass media -- South Africa , Endorsements in advertising -- South Africa , Music videos -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:8431 , http://hdl.handle.net/10948/d1020294
- Description: It has become conventional to use product placement in music videos. Big brands have started marketing themselves through product placement, for example, brands such as BMW, VitaminWater and Virgin Mobile. Placing products in music videos could be perceived as the brands linking themselves with celebrities. This product placement could either have a positive or negative effect on the celebrity and/or the brand. This research study aimed to provide the world of brands with insights into 18-28-year-olds’ perception of product placement in music videos, and whether placing their product in a music video where the audience is not partial to the celebrity, could be detrimental or not. This research study aimed to determine the selected sample’s (Nelson Mandela Metropolitan University students) perceptions of the product placement of three specific brands (VitaminWater, BMW and Virgin) in three specific music videos, namely: Jessie J and David Guetta “Laserlight” http://www.youtube.com/watch?v=syxd2n8S4AE JLo’s “Get on the Floor” http://www.youtube.com/watch?v=t4H_Zoh7G5A Lady Gaga and Beyoncé “Telephone” http://www.youtube.com/watch?v=GQ95z6ywcBY An electronic survey questionnaire was concluded with both closed and open-ended questions relating to the use of product placement in these three music videos. The survey questionnaire helped to determine what the selected sample’s perceptions were of each brand and celebrity, what they were after the respondents and seen the relationship between the brand and celebrity and finally, of product placement in general. A semiotic analysis of screen shots from each music video was conducted to determine what kind of nature the product placement occurred in in each music video.
- Full Text:
- Date Issued: 2013
- Authors: Haldane, Jayne
- Date: 2013
- Subjects: Product placement in mass media -- South Africa , Endorsements in advertising -- South Africa , Music videos -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:8431 , http://hdl.handle.net/10948/d1020294
- Description: It has become conventional to use product placement in music videos. Big brands have started marketing themselves through product placement, for example, brands such as BMW, VitaminWater and Virgin Mobile. Placing products in music videos could be perceived as the brands linking themselves with celebrities. This product placement could either have a positive or negative effect on the celebrity and/or the brand. This research study aimed to provide the world of brands with insights into 18-28-year-olds’ perception of product placement in music videos, and whether placing their product in a music video where the audience is not partial to the celebrity, could be detrimental or not. This research study aimed to determine the selected sample’s (Nelson Mandela Metropolitan University students) perceptions of the product placement of three specific brands (VitaminWater, BMW and Virgin) in three specific music videos, namely: Jessie J and David Guetta “Laserlight” http://www.youtube.com/watch?v=syxd2n8S4AE JLo’s “Get on the Floor” http://www.youtube.com/watch?v=t4H_Zoh7G5A Lady Gaga and Beyoncé “Telephone” http://www.youtube.com/watch?v=GQ95z6ywcBY An electronic survey questionnaire was concluded with both closed and open-ended questions relating to the use of product placement in these three music videos. The survey questionnaire helped to determine what the selected sample’s perceptions were of each brand and celebrity, what they were after the respondents and seen the relationship between the brand and celebrity and finally, of product placement in general. A semiotic analysis of screen shots from each music video was conducted to determine what kind of nature the product placement occurred in in each music video.
- Full Text:
- Date Issued: 2013
Nelson Mandela Metropolitan University students' perceptions of television advertisements for four SAB beer brands
- Authors: Tye, Robyn
- Date: 2013
- Subjects: Advertising -- Brewing industry , Advertising -- Alcoholic beverages , Television advertising -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:8439 , http://hdl.handle.net/10948/d1021122
- Description: This research study aimed to provide the South African beer industry and their advertising representative with insights into 18-28-year-olds’ perceptions of the communicated messaged in beer advertisements. This included the use of social and cultural references to attract their attention and the suggestions made by the advertisements about the consumption of beer in certain contexts. This research study aimed to determine the selected sample’s (NMMU students) perceptions of four South African Breweries beer brands, namely Castle Lager, Castle Lite, Carling Black Label and Hansa Pilsener, in terms of their use of references to social and cultural identity of males and females in South Africa within their television advertisements. The survey questionnaire helped determine what the selected sample’s perceptions were of each advertisement, and whether they fully understood the desired communicated message. It also helped to understand whether each advertisement captured their attention. A semiotic analysis of each advertisement was conducted to deconstruct the advertisements and to determine if they do contain elements of social and cultural identity in an attempt to sell products to their target audiences, or to affect the perceptions of the brand and drinking beer in general. This was achieved by examining the signs and imagery in each advertisement, looking specifically at the representamen, interpretant and object using Pierce’s model of a sign. , Hierdie navorsingstudie is daarop gemik om die Suid-Afrikaanse bierbedryf en sy adverteerders ‘n beter begrip te gee van verbruikers tussen die ouderdomme van 18-28, se waarnemings van die boodskappe in bieradvertensies. Dit sluit in die gebruik van sosiale en kulturele verwysings, wat veronderstel is om die verbruikers se aandag te trek, asook die suggesties wat deur die advertensies gemaak word met betrekking tot die verbruik van bier in ‘n bepaalde konteks. Die studie moes ook die gekose monster (“selected sample”), nl. die NMMU-student se persepsies bepaal van vier handelsname van die South African Breweries, naamlik Castle Lager, Castle Lite, Carling Black Label en Hansa Pilsener, en in watter mate daar in bieradvertensies oor die televisie verwys word na die sosiale en kulturele identiteit van Suid-Afrikaanse mans en vroue. Die meningspeiling het die groep se waarnemings van elke advertensie, asook of die boodskap wat gekommunikeer is ten volle verstaan word, ondersoek. Die vraelys kon ook vasstel of die advertensies hulle aandag getrek het. ‘n Semiotiese ontleding van elke advertensie is gedoen, om die advertensies te dekodeer en sodoende vas te stel of die adverteerders elemente van sosiale en kulturele identiteit gebruik het om hul produkte aan die teikenmarkte te verkoop, of om die idees rondom die handelsnaam en bier oor die algemeen te beïnvloed. Aan die hand van Peirce se semiotiese model is die tekens en beelde in elke advertensie bestudeer.
- Full Text:
- Date Issued: 2013
- Authors: Tye, Robyn
- Date: 2013
- Subjects: Advertising -- Brewing industry , Advertising -- Alcoholic beverages , Television advertising -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:8439 , http://hdl.handle.net/10948/d1021122
- Description: This research study aimed to provide the South African beer industry and their advertising representative with insights into 18-28-year-olds’ perceptions of the communicated messaged in beer advertisements. This included the use of social and cultural references to attract their attention and the suggestions made by the advertisements about the consumption of beer in certain contexts. This research study aimed to determine the selected sample’s (NMMU students) perceptions of four South African Breweries beer brands, namely Castle Lager, Castle Lite, Carling Black Label and Hansa Pilsener, in terms of their use of references to social and cultural identity of males and females in South Africa within their television advertisements. The survey questionnaire helped determine what the selected sample’s perceptions were of each advertisement, and whether they fully understood the desired communicated message. It also helped to understand whether each advertisement captured their attention. A semiotic analysis of each advertisement was conducted to deconstruct the advertisements and to determine if they do contain elements of social and cultural identity in an attempt to sell products to their target audiences, or to affect the perceptions of the brand and drinking beer in general. This was achieved by examining the signs and imagery in each advertisement, looking specifically at the representamen, interpretant and object using Pierce’s model of a sign. , Hierdie navorsingstudie is daarop gemik om die Suid-Afrikaanse bierbedryf en sy adverteerders ‘n beter begrip te gee van verbruikers tussen die ouderdomme van 18-28, se waarnemings van die boodskappe in bieradvertensies. Dit sluit in die gebruik van sosiale en kulturele verwysings, wat veronderstel is om die verbruikers se aandag te trek, asook die suggesties wat deur die advertensies gemaak word met betrekking tot die verbruik van bier in ‘n bepaalde konteks. Die studie moes ook die gekose monster (“selected sample”), nl. die NMMU-student se persepsies bepaal van vier handelsname van die South African Breweries, naamlik Castle Lager, Castle Lite, Carling Black Label en Hansa Pilsener, en in watter mate daar in bieradvertensies oor die televisie verwys word na die sosiale en kulturele identiteit van Suid-Afrikaanse mans en vroue. Die meningspeiling het die groep se waarnemings van elke advertensie, asook of die boodskap wat gekommunikeer is ten volle verstaan word, ondersoek. Die vraelys kon ook vasstel of die advertensies hulle aandag getrek het. ‘n Semiotiese ontleding van elke advertensie is gedoen, om die advertensies te dekodeer en sodoende vas te stel of die adverteerders elemente van sosiale en kulturele identiteit gebruik het om hul produkte aan die teikenmarkte te verkoop, of om die idees rondom die handelsnaam en bier oor die algemeen te beïnvloed. Aan die hand van Peirce se semiotiese model is die tekens en beelde in elke advertensie bestudeer.
- Full Text:
- Date Issued: 2013