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Perinatal experiences of pregnant undergraduate students of a higher education institution in the Eastern Cape

  • Authors: Simandla, Linda
  • Date: 2014
  • Language: English
  • Type: Thesis , Masters , MSc (Nursing Science)
  • Identifier: vital:11913 , http://hdl.handle.net/10353/d1016125
  • Description: Unplanned pregnancies among students are increasing annually. There are cases of students giving birth by themselves in the rooms within higher education institution residences unsupervised. Unsupervised births put the students at health risk as birth can complicate or even lead to death. The purpose of this study was to explore the perinatal experiences of undergraduate students whilst a pregnant student. In this study the researcher used a qualitative phenomenological approach within interpretive realism. The study was exploratory focusing on the need to understand how students experience perinatal care. Population for this study comprised of undergraduate students that had been pregnant within their undergraduate program which normally takes three to four years and were staying in the higher education institution residence at the time of pregnancy. A snowball sample was used. Data was collected from participants using unstructured in-depth interviews. For the study a total of 9 students were interviewed. Data was collected in English and Xhosa depending on preference. During pilot interviews the researcher established that the participants had difficulty in expressing themselves in English and used slang in spite of being registered for a degree. The interviews were conducted by the researcher and the data tape recorded. Permission to conduct a study at the higher education institution was sought from the Vice- chancellor, the Dean of Students and Research Ethics Committee To ensure trustworthiness of the study credibility, transferability, dependability and confirmability were adhered to. Four themes with several sub-themes emerged which were: Dynamics on awareness about existence of pregnancy. Support system for female students pertaining to reproductive health. Encountered psychosocial experiences. Coping strategies used by pregnant students. Excerpts were used to support the interpretation of perinatal experiences of the undergraduate students. Recommendations to the management of the higher education institution are to develop guidelines that ensure implementation of the pregnancy policy.
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  • Date Issued: 2014

Petrography, geochemistry and origin of atypical sedimentary-igneous contact relationships at the base of the Hotazel Formation around Middelplaats, Northern Cape Province, RSA

  • Authors: Terracin, Matthew Theodore
  • Date: 2014
  • Subjects: Petrology -- South Africa , Geochemistry -- South Africa , Igneous rocks -- South Africa , Manganese ores -- South Africa , Manganese ores -- Geology -- South Africa , Metasomatism (Mineralogy) , Potassium
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:5059 , http://hdl.handle.net/10962/d1012985
  • Description: In the Middelplaats mine area of the Kalahari manganese field, two drill holes (MP53 and MP54) intersected anomalously high-grade manganese ore sitting stratigraphically just above an igneous body (likely a dike or sill). Manganese ore located within approximate 5 meters of the contact with the underlying igneous rocks has been substantially metasomatically upgraded from 25 percent manganese, to over 40 percent whilst the dominant manganese species within the ore has been altered to hausmannite. This report demonstrates the metasomatic alteration is related to devolatilization (removal and/or remobilization of H₂O, CO₂ and CaO) due to contact metamorphism caused by the underlying igneous rocks. The Middelplaats mine is situated in the southwest corner of the Kalahari manganese field where the paleo basin shallows out and ends. Within the mine area, several stratigraphic units pinch out or are truncated by the side of the basin. This pinching out of lithological formations has led to the underlying Ongeluk Formation being in contact with the much younger units of the Hotazel Formation. Therefore, geochemical investigation into the nature and source of the igneous rocks was also undertaken to see if the rocks from the two drill holes were related to one another and/or the underlying Ongeluk Formation. Results of these geochemical studies have demonstrated that the Middelplaats igneous rocks (dolerites) from the two drill holes (MP53 and MP54) share a co-genetic source region. There is also reasonable geochemical evidence that the source region of the Middelplaats igneous rocks was substantially similar to the source region of the Ongeluk Formation. This may indicate that the source region of the Ongeluk Formation was reactivated at some later stage resulting in the emplacement of doleritic dikes or sills in the Middelplaats mine area. The Middelplaats igneous rocks were also found to have undergone a slight but pervasive potassic alteration; with most of the original plagioclase feldspar showing some level of replacement by a potassium enriched feldspar. Although no source for this potassic fluid was found, the devolatilization reaction within the manganese ore appears to have released some potassium into the surrounding rocks. This additional potassium may be responsible for some localized potassic alteration.
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  • Date Issued: 2014

Petrological, mineralogical and geochemical studies of Zhongchang and Yantiangou ultramafic intrusions from the Panxi region, Sichuan Province, S-W China. Metallogenic and Exploration implications

  • Authors: Malatji, Tsholofelo S
  • Date: 2014
  • Subjects: Mineralogy -- China , Geochemistry -- China
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:5057 , http://hdl.handle.net/10962/d1012951
  • Description: The Emeishan Large Igneous Province is one of the largest igneous provinces in the world. It is marked by basaltic extrusives that vary in Ti amount, classified as high Ti and low Ti. Several mafic to ultramafic intrusions occur in the province as well as granitic intrusions. The mafic-ultramafic intrusions are associated with mineralization; large layered utlramafic intrusions bearing Fe-Ti-V mineralization, while small dyke like or sill like intrusions contain Cu-Ni-PGE mineralization. The Zhongchang and Yantiangou mafic- ultramafic intrusions are small such intrusions bearing Ni-Cu-PGE mineralization. The deposits show a lot of similarities in source magma, evolution and mineralization. Parental magma for both deposits show alkaline tendencies, and a high Mg # indicative of primitive magma that underwent little crustal contamination. Mantle range Cu/Pd ratios show parental magma fertile in chalcophile elements and both deposits proceed to show depleted mantle normalized values as a result of sulphide saturation and subsequent mineralization. Mineralization in Zhongchang is hosted at the contact between peridotite and pyroxenite, interstitial to olivine and clinopyroxene. Alteration minerals include epidote and chlorite. High Cu/Pd ratios suggest earlier sulphide saturation and removal of a PGE sulphide, and a second sulphide saturation phase resulted in Ni-Cu mineralization. Mineralization in Yantiangou is hosted in biotite effectively following fractionation of olivine and clinopyroxene after which sulphide saturation occurred, precipitating sulphide minerals in biotite. Pyrite and millerite occur as a result of late stage hydrothermal alteration. Cu/Pd ratios are high indicating prior sulphide saturation occurred where PGE partitioned into sulphides. Ni/MgO ratios observed for both deposits indicate a state of sulphide under saturation where PGE partitioned into tellurides and bismurthinides and Ni into olivine. Average tenors for mineralized units are; Zhongchang at 5%Ni, 9% Cu, 4300ppb Pt and 2000ppb Pd, and Yantiangou having relatively lower tenors at 3.2%Ni, 6.5% Cu, 3000ppb Pt and 1300 ppb Pd.
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  • Date Issued: 2014

Platinum share prices and the Marikana tragedy: an event study

  • Authors: Sunga, Tapuwa Terence
  • Date: 2014
  • Subjects: Marikana (Rustenburg, South Africa) , Massacres -- South Africa -- Rustenburg , Stocks -- Prices -- South Africa , Stocks -- Prices -- Mathematical models
  • Language: English
  • Type: Thesis , Masters , MCom
  • Identifier: vital:1094 , http://hdl.handle.net/10962/d1013002
  • Description: An event study is an economic tool of analysis that has begun to gain popularity in recent empirical literature. It is a technique that gives a researcher the opportunity to map out the reaction of a firm's stock to an event, usually making use of daily or monthly data. However, up to this point, event study methodology has generally been applied to more traditional phenomena capable of affecting equity value, such as dividend and macroeconomic policy announcements, and there have only been a few exceptions to this. This study looks at what impact the tragic shootings at Lonmin mine in Marikana on August 16th 2012 had on the share prices of platinum mining firms based in South Africa using event study methodology. It makes use of the technique to investigate how the share prices responded to the tragedy over a number of trading days, including the day of the shootings. To be best of our knowledge, no attempt has been made to analyse the impact on share prices using events of this nature. For the investigation, daily returns data was used for each firm. The abnormal returns and cumulative abnormal returns to each were then calculated and compared with their respective expected returns in order to determine whether investors in the shares of that particular firm reacted positively, negatively or not at all. The evidence found suggests that tragedies of this nature are capable of influencing share prices in the same manner as more traditional economic phenomena. Overall, only one firm was found to have been negatively affected by the shootings in a persistent manner, while the shares of the other firms examined reacted in a manner that was positive overall, but varied according to individual firm characteristics such as size. These finding conformed to our a priori expectations. In addition, the results also confirm the benefits of applying event study methodology to a wide variety of phenomena that fall outside the boundaries usually associated with business.
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  • Date Issued: 2014

Political and religious identities : an exploration of changing identities amongst religious leaders in the Northern areas of Port Elizabeth

  • Authors: Abrahams, Andre John
  • Date: 2014
  • Subjects: Religion and politics -- South Africa -- Port Elizabeth , Identification (Religion)
  • Language: English
  • Type: Thesis , Masters , MA
  • Identifier: vital:8314 , http://hdl.handle.net/10948/d1020087
  • Description: The political transition from the oppressive apartheid system to post-apartheid South Africa has brought considerable change to the political climate. As a result of this transition, political and religious identities were also affected. This study explores the changing identities amongst religious leaders particularly in the Northern Areas of Port Elizabeth. The research has also explored how the advent of democracy has affected the political perspectives of these religious leaders who were ministering during apartheid. A qualitative research approach was used to frame the study, which employed an exploratory research design so as to understand the current political identities of these religious leaders. The sample of the study was purposively selected using the snowball sampling technique. As a means of collecting data in-depth interviews were conducted. The themes emanating from the study were recognised as being: - Church leaders reflecting on the political climate apparent in the Northern Areas of Port Elizabeth - Church leaders reflect reasons for limited political action from certain segments of the community sighting fear and poverty as central tenets. - Church leaders reveal close link between the church and politics during the apartheid period - The activities of the church in opposing the apartheid structure - Participants reflect on the role of Church leaders specifically during this period. - Church leaders articulate their disappointment in the political environment since 1994. - Church leaders reflect how race has affected the political climate since 1994 - The changing role of the church since 1994 - The growing silence on current political issues since 1994 Based on the identified themes conclusions could be drawn on the current political and religious identities of religious leaders compared to those adopted during the apartheid period. The outcome showed that religious leaders within the Northern Areas of Port Elizabeth are disappointed in the current political government but despite this have remained silent on political issues unlike the visible activism and vocal upheaval waged against the apartheid system.
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  • Date Issued: 2014

Political grey : areas of ambiguity and contradiction

  • Authors: Koekemoer, Carmen
  • Date: 2014
  • Subjects: Politics in art , Art -- Political aspects -- South Africa , Leadership in art , Portraits, African , Portraits, European , Art and revolutions -- South Africa
  • Language: English
  • Type: Thesis , Masters , MFA
  • Identifier: vital:2492 , http://hdl.handle.net/10962/d1013136
  • Description: This Master of Fine Arts submission, consisting of a thesis titled ‘Political Grey: Areas of Ambiguity and Contradiction’ accompanied by an exhibition titled ‘Positions’, encompasses the concept of leadership while uncovering and expressing its ‘grey areas’ in a contemporary and undefined moment in South Africa. The concept of leadership has been complicated throughout the thesis in terms of how it is conceptualised in a traditional royal African art context as well as how Leader-Figures have been and are portrayed in both Western and African portrait genres. The notion that the new is built upon the old is continued throughout my thesis and is evident in the accompanying body of work. This notion is expressed on a number of levels: by the re-contextualisation of the print medium; the creative processes described as ‘postproduction’ which I use in my work; as well as that which is described as a ‘post-transitional’ moment. The recent political history of the country is considered, with reference made to the anti-apartheid movement and resistance art produced. Printmaking, viewed as an archetypal medium for resistance, is discussed, with reference made to its socio-political role during the 1980s as well as to the extent to which it continues to be used by contemporary artists in a different realm of conflict and change. This is demonstrated by the shift from the medium as a tool for protest to the medium as an instrument of political irony and pointed commentary.
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  • Date Issued: 2014

Polynuclear biomolecular-supported rare earth coordination compounds : towards a new generation of lanthanide-based drugs

  • Authors: Clark, Candyce
  • Date: 2014
  • Subjects: Polyols , Hydroxyl group , Rare earth metals
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:10439 , http://hdl.handle.net/10948/d1020778
  • Description: Galactitol and cis,cis,cis-1,3,5-cyclohexanetriol are polyols that are ideal examples of model compounds for ligands with lanthanide ions as they have their hydroxyl groups in favourable steric arrangement. Several complexes were synthesised with both lanthanide chloride and lanthanide nitrate salts with galactitol, and a variety of structures, both polymeric and monomeric, were observed. In all these complexes, galactitol acted as a bridging molecule between the lanthanide ions. A notable difference was the lanthanum chloride–galactitol complex that showed both chloride and galactitol bridging. The lanthanide nitrate salts formed only polymeric complexes with galactitol. Not all of the complexes showed nitrate ions coordinated to the metal centre, and in the neodymium nitrate– galactitol complex, which shows both monodentate and bidentate coordination of the nitrate groups. The coordination of the nitrate ions was confirmed using both XRD and IR analysis. Two complexes with lanthanide chloride salts and cis,cis,cis-1,3,5-cyclohexanetriol were synthesised and analysed. Lanthanum chloride formed a polymeric complex, which showed extensive chloride bridging between the metal centres. Praseodymium chloride formed a dimeric complex. All complexes were analysed with single-crystal X-ray diffraction, 1H NMR, 13C NMR and IR spectroscopy.
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  • Date Issued: 2014

Portfolio of compositions

  • Authors: Simpson, Wayne Charles
  • Date: 2014
  • Subjects: Composition (Music) , Music
  • Language: English
  • Type: Thesis , Masters , MMus
  • Identifier: http://hdl.handle.net/10948/7385 , vital:21343
  • Description: This essay will address the evolving nature of, and diversity, in method types of musical analysis. It will explore the connection between methods and approach types to musical analysis and related conceptions of what constitutes meaning in music. These methods will be largely understood as operating inside given parameters of musical meaning. Following a short history of some of the developments concerning structure in music from the 17th to late 19th century, I will discuss some of the dominant analytical methods and aim to highlight common features between them. I will discuss the different approaches these methods employ and highlight areas where a given method might be seen as using more than one approach. I will then investigate some approaches to composition that fall outside the conventional view of musical meaning and ultimately suggest the application of an eclectic model to musical analysis.
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  • Date Issued: 2014

Possible scenarios for Africa's economic futures towards 2055

  • Authors: Giyose, Dorrington
  • Date: 2014
  • Subjects: Economic forecasting -- South Africa , Future, The
  • Language: English
  • Type: Thesis , Masters , MBA
  • Identifier: vital:8931 , http://hdl.handle.net/10948/d1021188
  • Description: This academic discourse is a research treatise that is submitted in fulfilment of the requirements for the Masters in Business Administration (MBA) degree at the Nelson Mandela Metropolitan University (NMMU). Purpose – The purpose of this treatise is to develop four possible scenarios for Africa’s economic futures over the next 40 years, i.e. towards 2055. This study will expose the possible, probable, plausible, and preferable (desirable) scenarios for Africa towards 2055. Design/Methodology/Approach – This study employs a Futures Studies methodology that is known as scenario planning. The key variables of the scenarios are clustered as follows: Good governance and good economic growth; Good governance and bad economic growth; Bad governance and bad economic growth; as well as bad governance and good economic growth. Each of these scenarios begin with the current state of affairs in Africa. As such, the four scenarios in this study are informed by the current affairs in African countries as is internationally observed by scientists, researchers, as well as global views and opinions. Practical implications – This academic discourse provides useful insight into the causality relationship between the political, economic, sociological, technological, ecological, as well as legal factors (PESTEL factors) on the continent and the possible scenarios for Africa’s economic futures towards 2055. The aforementioned causality relationship between the abovementioned variables allows for insight into the drivers for change for Africa as well as in what way to anticipate these changes in accordance with scenario planning. Originality/Value: This treatise looks at the economic futures of Africa over the next 40 years from the point of view of African planners and African decision-makers.
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  • Date Issued: 2014

Potential for human exposure to Beta-N-methylamino-L-alanine in a freshwater system

  • Authors: Scott, Laura Louise
  • Date: 2014
  • Subjects: Water quality biological assessment , Cyanobacteria , Neurotoxic agents
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: http://hdl.handle.net/10948/5159 , vital:20816
  • Description: β-N-methylamino-L-alanine (BMAA) is a non-proteinogenic amino acid associated with human neurodegenerative diseases. The content of BMAA in cyanobacteria is modulated by nitrogen in laboratory cultures. In order to evaluate the potential for human exposure, the nitrogen modulation of BMAA content needed verification in a natural environment. In accordance with laboratory culture studies, data presented in this study show that combined nitrogen was the most significant modulator of both cellular microcystin (MC) and BMAA content in phytoplankton in an environmental cyanobacterial bloom. While BMAA is produced upon nitrogen deprivation, MC is only produced at a specific nitrogen threshold where the rate of increase of nitrogen in the cell exceeds the carbon fixation rate. As BMAA and MC were detected in phytoplankton sourced from the Hartbeespoort Dam reservoir, the transfer of these cyanotoxins to organisms of higher trophic levels was investigated. Both BMAA and MC were detected at high concentrations in the liver and muscle tissue of fish sourced from the Hartbeespoort Dam reservoir indicating that consumption of fish from this reservoir constitutes a serious risk of exposure to cyanotoxins. In addition to the dietary exposure route to BMAA, two recent studies reported a correlation between Amyotrophic Lateral Sclerosis (ALS) incidence and the potential for aerosol exposure to cyanobacteria. With the absence of any evidence of the systemic distribution of BMAA following inhalation, an evaluation of the potential exposure risk associated with living in close proximity to this reservoir was deemed premature. A laboratory experiment investigating the effect and systemic fate of inhaled aerosolised BMAA was therefore conducted in order to determine the feasibility of inhalation as a potential BMAA exposure route. Data from the rat inhalation exposure study, however, showed that in rats BMAA inhalation may not constitute a significant mechanism of toxicity at environmental BMAA levels.
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  • Date Issued: 2014

Poverty alleviation through income generation: Khuphuka Bakery in Stutterheim

  • Authors: Nini, Linda Frances
  • Date: 2014
  • Subjects: Poverty -- Economic aspects -- South Africa -- Eastern Cape , Community development -- South Africa -- Eastern Cape , Economic development projects -- South Africa -- Eastern Cape
  • Language: English
  • Type: Thesis , Masters , MA
  • Identifier: http://hdl.handle.net/10948/8934 , vital:26444
  • Description: Income generating projects play a significant role in the lives of rural people and make a significant contribution to rural development in South Africa. Although, income generating projects play a major contribution to rural development, problems of funding for such projects and the high levels of unskilled personnel to run those projects is still a challenge in most, if not all rural areas of South Africa. With the government failing to meet employment demands of most unskilled people and with most vacancies demanding work experiences of up to 10 years, most people especially the youth have found themselves unqualified for such vacancies hence they have resorted to projects in their communities in way boosting family incomes and at the same time alleviating poverty. Therefore this study has undertaken with the objectives of assessing income generating projects in relation to its contribution to rural development in the study area. The sustainable livelihoods and basic needs approaches were used to assess the contribution of income generating projects in poverty alleviation in rural areas. Such approaches to rural development can help in reducing the poverty gap that exists in society and at the same time help achieve sustainable rural development. Findings show that there are a number of challenges and constraints that are experienced by the Amahlathi community, like poverty in the form of poor water and sanitation services, education and health services and landlessness. Moreover, serious service delivery protests are a daily occurrence, corruption at local government level have all hindered the successful support of the government needed to boost these rural income generating projects. To have a successful rural development, there is need for the government to support these projects in the form of skills training programmes or finance. The study recommends that a serious state intervention should occur coupled with the visitation and revitalization of the RDP programme correct its mistakes.
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  • Date Issued: 2014

Practices of early childhood development (ECD) practitioners for children from three to five years: a case of three early childhood development centres in the Buffalo City Municipality

  • Authors: Keir, Charlene (Ying-Ling)
  • Date: 2014
  • Subjects: Education, Preschool -- South Africa -- Eastern Cape , Preschool teachers -- South Africa -- Eastern Cape
  • Language: English
  • Type: Thesis , Masters , M Ed
  • Identifier: vital:16208 , http://hdl.handle.net/10353/d1016269 , Education, Preschool -- South Africa -- Eastern Cape , Preschool teachers -- South Africa -- Eastern Cape
  • Description: This study investigates practices of early childhood development (ECD) practitioners for children from three to five years in three ECD centres situated within the municipal boundaries of Buffalo City, East London. It does so by posing the following questions: What are the practices of ECD practitioners for children from three to five years in developing learners’ oral language and physical intelligence. This study, using a qualitative approach and Qualitative methods for data collection were used, that is semi-structured interviews and classroom observations. The findings reveal that for oral language development, storytelling and children sharing news seemed to be the most used practices. However, the practitioners in this study seemed to severely lack understanding of their selected practices. Practices for developing children’s physical intelligence included free play, which was unsupervised. Practitioners seemed not to have an understanding of constructive play which is very important for the development of learners’ physical intelligence. One of the factors reported to be the cause of the poor quality of their practices was the critical shortage of funding for practitioners’ professional training. Moreover, shortage of funds also contributed to poor and inappropriate infrastructure and a lack of resources and teaching aids. This study, therefore, recommends that the quality of ECD programmes could be one of the prime contributors to the quality and effectiveness of ECD provisioning.
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  • Date Issued: 2014

Practitioners' views of the Griffiths scales : informing the revision process

  • Authors: Samuel, Candice
  • Date: 2014
  • Subjects: Psychological tests for children -- South Africa , Griffiths Developmental Scales , Child development -- Testing
  • Language: English
  • Type: Thesis , Masters , MA
  • Identifier: vital:9975 , http://hdl.handle.net/10948/d1021062
  • Description: The Association for Research in Infant and Child Development (ARICD) is currently in the process of revising the Griffiths Scales. When revising measures, obtaining practitioners’ views on the measure is common practice. The purpose of the present study was to explore the themes emerging from a group of practitioners surveyed on their opinions about the Griffiths Scales in order to inform the Griffiths Scales revision process. A qualitative, survey methodology was used. A survey questionnaire was designed and distributed by the ARICD to ascertain practitioners’ and researchers’ views on assessing child development, insights into practicalities and the usability of the Griffiths Scales, as well as their opinions on potential changes and improvements. Data were also gathered on the frequency, purpose and age range with which practitioners used the Griffiths Scales. The questionnaire was distributed to Registered Griffiths Scales Users (practitioners) via the ARICD members’ email distribution list. The practitioners and researchers were either current or past users of the Griffiths Scales. Eighty-five completed questionnaires were returned of which 52 were from current, regular users of the Griffiths Scales and 33 were from non-users. The data obtained from the 85 returned questionnaires were analysed in the present research study using thematic analysis to extract themes for both users and non-users of the Griffiths Scales. The results of the data analysis revealed four over-arching themes, namely, purpose and use of the Griffiths Scales; domains, content, and structure of the Griffiths Scales; psychometric properties, standardisation, and norms; and merits, limitations, and improvements. Based on the themed views of practitioners, recommendations were made regarding the support for the next revision cycle as well as strengths of the Griffiths Scales that should be retained and improvements and additions needed. It is hoped that these recommendations will guide aspects of the revision process. Consequently, the recommendations have been submitted to the Project Board.
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  • Date Issued: 2014

Predation by alien largemouth bass, Micropterus salmoides Lacepéde 1802 (Centrarchidae: Perciformes), on indigenous marine fish species in the Kowie System, South Africa

  • Authors: Magoro, Mandla Leon
  • Date: 2014
  • Subjects: Largemouth bass -- South Africa -- Port Alfred , Largemouth bass -- Food -- South Africa -- Port Alfred , Estuarine fishes -- South Africa -- Port Alfred , Estuarine fishes -- Predators of , Centrarchidae , Fishes -- Effect of human beings on
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:5851 , http://hdl.handle.net/10962/d1011939 , Largemouth bass -- South Africa -- Port Alfred , Largemouth bass -- Food -- South Africa -- Port Alfred , Estuarine fishes -- South Africa -- Port Alfred , Estuarine fishes -- Predators of , Centrarchidae , Fishes -- Effect of human beings on
  • Description: Estuaries serve as nursery areas for a large number of estuary-associated fish species. Some of these taxa also use river catchments as nursery areas. During the upstream migration of this latter group, the juveniles are prone to predation by native and alien predatory fish inhabiting the system. The rate of invasion of ecosystems by alien organisms can be directly linked to anthropogenic influences, including both intentional and unintentional introductions by alien organisms into new regions. The largemouth bass, Micropterus salmoides, is a facultative piscivorous fish that has been successfully introduced worldwide for the main purpose of sport fishing. Where introduced, it has been found to negatively impact native fish and invertebrate species through predation, competitive exclusion and displacement of indigenous fish species. The aim of this thesis was to investigate the predatory impact of largemouth bass on the estuary-associated Cape moony Monodactylus falciformis, Cape stumpnose Rhabdosargus holubi and freshwater mullet Myxus capensis in the lower Kowie River of the Eastern Cape Province, South Africa. A combination of approaches was employed during this study. Stomach contents, stable isotopes and fatty acid analyses were employed for the reconstruction of the diet of largemouth bass. Acoustic telemetry was used to elucidate largemouth bass movements, particularly their ability to enter the upper reaches of the Kowie Estuary. Stomach contents and stable isotopes analyses showed that M. salmoides exhibit an ontogenic shift in diet, with small and medium sized individuals consuming the higher proportion of fish prey, while large sized individuals mostly consumed invertebrates such as crabs (Potamonautes sidneyi) and Odonata larvae, while consuming only a small proportion of estuary-associated fish. Fatty acid analysis only showed a direct connection between the fatty acid profiles of largemouth bass and those of M. capensis and M. falciformis. The acoustic telemetry results indicated that some M. salmoides individuals periodically move into the upper reaches of the estuary following river flood events. The results obtained from all these approaches highlight the risk posed by largemouth bass introductions on indigenous fish species, particularly those that enter the areas occupied by these top predators.
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  • Date Issued: 2014

Preparation and characterisation of nanocomposites of biomass and montmorillonite clay for use as biofuels

  • Authors: Nyamutswa, Lavern Tendayi
  • Date: 2014
  • Language: English
  • Type: Thesis , Masters , MSc (Chemistry)
  • Identifier: vital:11345 , http://hdl.handle.net/10353/d1019779
  • Description: The aim of the study was to prepare composites of clay and biomass which burn longer than unmodified biomass. Montmorillonite clay was converted to mono-ionic clay by ion exchange with sodium using sodium chloride solution. The mono-ionic clay was organically modified with an organic surfactant, methyl triphenyl phosphonium bromide. Nanocomposites were then prepared by combining the modified forms of the clay with sawdust. The three forms of clay used for the formation of composites were unmodified montmorillonite, mono-ionic montmorillonite and organically modified montmorillonite. The solution blending method was used to make the nanocomposites. FT-IR and XRD analysis showed that organic surfactant increases the interlayer space of the clay since it is bulkier than the inorganic cations that are naturally present in the interlayer space of montmorillonite. The combination of clay and sawdust resulted in the formation of exfoliated nanocomposites as shown by the absence of peaks in the low 2 theta angle in the x-ray diffraction data of the nanocomposite. The nanocomposite which was made from sawdust and 1% organically modified clay showed the best results in terms of burning time and thermal stability, as well as giving a calorific value closest to unmodified sawdust and the least amount of residue.
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  • Date Issued: 2014

Prevalence and molecular epidemiology of extended-spectrum beta-lactamase-producing and carbapenem-resistant enterobacteriaceae, acinetobacter baumannii and pseudomonas aeruginosa in Port Elizabeth

  • Authors: Gqunta, Kwanele
  • Date: 2014
  • Subjects: Beta lactamases , Enterobacteriaceae -- South Africa -- Port Elizabeth
  • Language: English
  • Type: Thesis , Masters , MSc
  • Identifier: vital:10353 , http://hdl.handle.net/10948/d1020944
  • Description: Multidrug resistant (MDR) extended-spectrum β-lactamase (ESBL)-producing and carbapenem-resistant Gram-negative bacteria have become an international health issue limiting treatment options. The objective of this study was to determine the prevalence of carbapenem-susceptible (CS) and carbapenem-resistant (CR) Enterobacteriaceae, Acinetobacter baumannii and Pseudomonas aeruginosa and investigate clinical isolates for their resistant genes/ determinants. A total of 98 bacterial isolates (59 CS and 39 CR) were collected between February 2012 and February 2013 at NHLS, after being recovered from various clinical specimens from PE hospital complex. Antimicrobial susceptibility testing was performed using the VITEK 2® system, E-test and microbroth dilution method. PCR and DNA sequencing were used to investigate: (i) ESBLs: CTX-M, TEM, SHV and OXA-1; (ii) plasmidmediated quinolone resistance (PMQR) genes: qnrA, qnrB, qnrC, qnrD, qnrS, qepA and aac(6’)-lb-cr; (iii) Escherichia coli sequence type 131 (ST131); (iv) carbapenemases: NDM, VIM, IMP, KPC, BIC, SME, IMI, NMC-A, GES, OXA-23, OXA-24, OXA-48, OXA-51 and OXA-58; and (v) insertion sequence ISAba1 upstream blaOXA-23/-24/-51/-58 genes. Porin loss in CR isolates was determined by SDSPAGE while genetic relatedness between E. coli ST131 isolates was determined by pulsed-field gel electrophoresis (PFGE). MDR ESBL-producing Enterobacteriaceae (mainly K. pneumoniae) and CR A. baumannii isolates were recovered from neonatal/ infant specimens. The majority of CS and CR isolates were MDR, possessing multiple ESBL genotypes (CTX-M, TEM, SHV and OXA-1). ESBL variants identified included: CTX-M-1, CTX-M-3, CTX-M-15, CTX-M-22, CTX-M-9, CTX-M-14, TEM-1, SHV-1, SHV-11 and OXA-1. PMQRs identified included: aac(6’)- lb-cr, qnrB1, qnrB2, qnrB13 and qnrS1. Twelve of 21 (57.1 percent) E. coli isolates belonged to the ST131 clonal complex and were genetically diverse, mainly producing CTX-M-15. Carbapenem resistance mechanisms identified included: (i) OXA-23 preceded by ISAba1 in 10 A. baumannii and 2 P. aeruginosa isolates; (ii) IMI-2 carbapenemase in an E. asburiae isolate; and (iii) combination of porin loss and ESBL production in 1 K. pneumoniae and 1 E. coli isolate. This is the first report in South Africa describing the occurrence of CTX-M-9, CTX-M-22 and IMI-2 among Enterobacteriaceae; CTX-M-15 in A. baumannii; and OXA-23 in combination with OXA-51 in P. aeruginosa. However, resistance determinants could not be detected for 24 carbapenem-resistant isolates which requires further investigation.
  • Full Text:
  • Date Issued: 2014

Prevalence and pathogenicity of vibrios in treated final effluents of selected wastewater treatment plants in the Amathole District Municipality of Eastern Cape Province of South Africa

  • Authors: Badela, Andiswa Unathi
  • Date: 2014
  • Subjects: Sewage disposal plants -- South Africa -- Eastern Cape , Whole effluent toxicity testing -- South Africa -- Eastern Cape , Escherichia coli -- South Africa -- Eastern Cape , Bacterial diseases -- Pathogenesis
  • Language: English
  • Type: Thesis , Masters , MSc (Microbiology)
  • Identifier: vital:11284 , http://hdl.handle.net/10353/d1019774 , Sewage disposal plants -- South Africa -- Eastern Cape , Whole effluent toxicity testing -- South Africa -- Eastern Cape , Escherichia coli -- South Africa -- Eastern Cape , Bacterial diseases -- Pathogenesis
  • Description: Waterborne diarrhoeal infections continue to be a major health setback in developing countries, especially in rural areas which lack adequate supply of portable water and sanitation facilities. Globally, waterborne diarrhoeal infections occur with an estimated mortality rate of 10–25 million deaths per year, 95% of which are children under the age of 5 years. The Vibrio species is one of the major groups of enteric pathogens that are responsible for diarrhoeal infections. Many strains of these bacterial species continue to cause epidemics of diarrhoea throughout the world. In this study, the prevalence of Vibrio pathogens in wastewater final effluents was assessed. Wastewater final effluent and discharge point samples were collected monthly between September 2012 and August 2013. All samples were collected aseptically using sterile 1 L Nalgene bottles containing 0.5 ml of sterile sodium thiosulphate solution and transported on ice to the laboratory for analyses within 6 h of collection. The membrane filtration method was used for enumeration of presumptive Vibrio densities on thiosulfate citrate bile salt (TCBS) agar plates. Polymerase chain reaction (PCR) was then used to confirm the identities of the presumptive Vibrio species using the species-specific primers. The confirmed isolates were further subjected to molecular characterization to confirm their respective pathotypes. Presumptive Vibrio densities varied from 0 to 2.11 × 102 cfu/100 ml. Out of 300 confirmed Vibrio isolates; 13.3% (40/300) were Vibrio fluvialis, 22% (66/300) were confirmed to be Vibrio parahaemolyticus, and 24.7% (74/300) proved to be Vibrio vulnificus, and 40% (120/300) were other Vibrio species which were not assessed for in this study. The strains of Vibrio fluvialis were found to exhibit 100% resistance to Polymixin and Tetracycline. However, Gentamicin was active against all the three Vibrio species selected for the purpose of this research. The recovery of Vibrio species in the discharged effluents throughout the sampling period even in adequately disinfected effluents is not acceptable considering the fact that Vibrio is a pathogenic bacterium. The findings of this study underline the need for constant monitoring of the microbiological qualities of discharged effluents and might also be suggestive for a review of the disinfection methods used at the treatment works.
  • Full Text:
  • Date Issued: 2014

Prevalence and resistance gene mutations of multi-drug resistant and extensively drug resistant mycobacterium tuberculosis in the Eastern Cape

  • Authors: Hayes, Cindy
  • Date: 2014
  • Subjects: Multidrug-resistant tuberculosis -- South Africa -- Eastern Cape , Mycobacterium tuberculosis -- South Africa -- Eastern Cape , Microbial mutation
  • Language: English
  • Type: Thesis , Masters , MTech
  • Identifier: vital:10125 , http://hdl.handle.net/10948/d1020374
  • Description: The emergence and spread of multi-drug resistant (MDR-TB) and extensively drugresistant tuberculosis (XDR-TB) are a major medical and public problem threatening the global health. The objectives of this study were to (i) determine the prevalence of MDR-TB and XDR-TB in the Eastern Cape; (ii) analyze patterns of gene mutations in MDR-TB and (iii) identify gene mutations associated with resistance to second line injectable drugs in XDR-TB isolates. A total of 1520 routine sputum specimens sequentially received within a period of 12 months i.e. February 2012 to February 2013 from all MDR-TB and XDR-TB patients treated by Hospitals and clinics in the Eastern Cape were included in this study, of which 1004 had interpretable results. Samples were analyzed with the Genotype MTBDRplus VER 2.0 assay kit (Hain Lifescience) for detection of resistance to Rifampicin and Isoniazid while solid and liquid culture drug susceptibility tests were used for ethambutol, streptomycin, ethionamide, ofloxacin, capreomycin and amikacin. PCR and sequence analysis of short regions of target genes gyrA, (encode subunit of DNA topoisomerase gyrase), rrs (16S rRNA) and tlyA (encodes a 2’-O-methyltransferase) were performed on 20 XDR-TB isolates. MTBDRplus kit results and drug susceptibility tests identified 462 MDR-TB, 284 pre-XDR and 258 XDR-TB isolates from 267 clinics and 25 hospitals in the Eastern Cape. There was a high frequency of resistance to streptomycin, ethionamide, amikacin, ofloxacin and capreomycin. Mutation patterns indicated differences between the health districts as well as differences between the facilities within the health districts. The most common mutation patterns observed were: (i) ΔWT3, ΔWT4, MUT1 [D516V+del515] (rpoB), ΔWT, MUT1 [S315T1] (katG), ΔWT1 [C15T] (inhA) [39 MDR, 204 XDR-TB and 214 pre XDR-TB isolates], (ii) ΔWT8, MUT3 [L533P+S531L] (rpoB), ΔWT, MUT1 [S315T1] [145 MDR, 18 pre-XDR and 3 XDR-TB solates] and (iii) ΔWT3, WT4 [D516Y+del515] (rpoB), ΔWT, MUT1 [S315T1] (katG) [75 MDR, 1 pre-XDR and 7 XDR-TB isolates]. Mutations in inhA promoter regions were strongly associated with XDR-TB isolates. Two thirds (66.6 percent (669/1004) of the isolates had inhA mutations present with 25.4 percent (170/669) found among the MDR isolates, 39.2 percent (262/669) among the pre-XDR isolates and 35.4 percent (237/669) among the XDR-TB isolates, which implies that these resistant isolates are being spread by transmission within the community and circulating in the province. There was good correlation between XDR-TB drug susceptibility test results and sequence analyses of the gyrA and rrs genes. The majority of XDR-TB isolates contained mutations at positions C269T (6/20) and 1401G (18/20) in gyrA and rrs genes respectively. Sequence analysis of short regions of gyrA and rrs genes may be useful for detection of fluoroquinolone and amikacin/ kanamycin resistance in XDR-TB isolates but the tlyA gene is not a sensitive genetic marker for capreomycin resistance. This study highlighted the urgent need for the development of rapid diagnostics for XDR-TB and raised serious concerns regarding ineffective patientmanagement resulting in ongoing transmission of extremely resistant strains of XDRTB in the Eastern Cape suggesting that the Eastern Cape could be fast becoming the epicenter for the development of Totally Drug-resistant Tuberculosis (TDR-TB) in South Africa.
  • Full Text:
  • Date Issued: 2014

Prevalence of pathogenic Escherichia coli strains in the final effluents of four wastewater treatment plants in the Eastern Cape Province of South Africa

  • Authors: Seti, Nozuko Zukiswa
  • Date: 2014
  • Subjects: Escherichia coli -- South Africa -- Eastern Cape , Bacterial diseases -- Pathogenesis , Sewage -- Purification -- South Africa -- Eastern Cape , Sewage disposal plants -- South Africa -- Eastern Cape , Whole effluent toxicity testing -- South Africa -- Eastern Cape
  • Language: English
  • Type: Thesis , Masters , MSc (Microbiology)
  • Identifier: vital:11286 , http://hdl.handle.net/10353/d1019808 , Escherichia coli -- South Africa -- Eastern Cape , Bacterial diseases -- Pathogenesis , Sewage -- Purification -- South Africa -- Eastern Cape , Sewage disposal plants -- South Africa -- Eastern Cape , Whole effluent toxicity testing -- South Africa -- Eastern Cape
  • Description: Water is an essential need that stimulates health and well being. Increase in population size and urbanization negatively affect water resources due to high demands of effluent outputs. Wastewater is an important reservoir for Escherichia coli and can present significant acute toxicity if released into receiving water sources without being adequately treated. E. coli is used as indicator organism for the detection of faecal contamination. These strains have been considered to be one of the primary causes of diarrhoeal infections worldwide. The present study was conducted between September 2012 and June 2013 to assess the prevalence of pathogenic E. coli strains in the final effluents of four wastewater treatment plants in Chris Hani and Buffalo City Municipalities in the Eastern Cape Province of South Africa. Standard membrane filtration technique was used for bacteriological analysis and molecular based technique was used for identification of E. coli pathotypes. The results were recorded in colony forming units/100 ml. Faecal coliforms ranged between 0-9.6×10³ CFU/100 ml for the wwtp-Q and E. coli densities ranged between 0-8.4×10³ CFU/100ml. Faecal coliforms ranged between 4×10²-9.7×10³ CFU/100 ml for wwtp-M and E. coli densities ranged between 1.2×10¹-8.4×10³ CFU/100 ml. The wwtp-E showed to have bacterial counts of faecal coliforms ranging between 4.0×10³-8.2×10³ CFU/100 ml and E. coli densities ranging between 3.5×10¹-7.1×10³ CFU/100 ml. The WWTP-K in this study was only assessed for the presence of E. coli. Faecal coliforms were assessed by the other members of the group. This plant showed to have E. coli densities ranging between 0-7.5×10²CFU/100 ml. A total of 200 presumptive E. coli isolates were subjected to screening by conventional PCR in which (29%) of the wwtp-M isolates were positively identified as E. coli, (16%) of the wwtp-K, (22%) of the wwtp-Q and (34%) of the wwtp-E isolates were positively confirmed as E. coli. A total of 100 randomly selected E. coli isolates were characterised into different pathotypes. (16%) of positive isolates were detected as EPEC and 11% were detected as UPEC strains. There was no detection for the ETEC strains. Antibiotic susceptibility patterns of E. coli strains showed high levels of resistance to Penicillin G, Erythromycin, Tetracycline and Sulfamethoxazole. High levels of Susceptibility were observed in antibiotics such as Chloramphenicol, Amoxicillin and Tetracycline. The results of this study reveal that the plants were above the recommended Standard limit of zero CFU/100 ml for effluents meant to be discharge into receiving water sources. This study reveals inadequacy of the plants studied to produce effluents of acceptable quality.
  • Full Text:
  • Date Issued: 2014

Primary caregivers' experiences of caring for HIV infected adolescents

  • Authors: Matebese, Dineo
  • Date: 2014
  • Subjects: Caregivers -- Family relationships , AIDS (Disease) -- Patients -- Family relationships , HIV-positive youth
  • Language: English
  • Type: Thesis , Masters , MA
  • Identifier: http://hdl.handle.net/10948/5745 , vital:20994
  • Description: HIV/AIDS has affected families in a profound and tragic way. Children whose parents have succumbed due to HIV/AIDS related infections have to be cared for by their relatives. This study focused on the experiences of primary caregivers of adolescents who are living with HIV in a semi-rural area of Eastern Cape Province, Grahamstown. There is a dearth of literature that is addressing the well-being, experiences and challenges of primary caregivers of adolescents living with HIV. Adolescence is a complex life stage during which adolescents usually present challenging behaviours and are grappling with moral issues. Caregiving to these youngsters who are burdened with HIV infection places additional demands on caregivers, especially so when they are experiencing poverty and poor social and welfare support. A qualitative study was most appropriate to explore and describe the experiences of adolescents infected with HIV. The population of this study was the primary caregivers of adolescents caring for adolescents with HIV in the area of Grahamstown, who are between 11 to 19 years old. A purposive sample of nine primary caregivers of adolescents living with HIV was selected. Data was collected by conducting individual interviews, using a semi-structured interview schedule. Data was analysed thematically according to prescribed theoretical guidelines. Data was verified by means of guidelines for the truth value, applicability, consistency, and neutrality of the study. The findings of the study indicated that primary caregivers experience that caring for adolescents who are living with HIV places a huge burden due to adolescents' negative behaviour, HIV stigma and discrimination, poor support from their families, being confronted with adolescents' emotional issues that they are not capacitated to deal with, and no adequate community resources to assist them. The challenges primary caregivers experience lead to negative effects on their health status. The findings of the study could be useful for informing intervention programmes that are targeting this group and policy development, and implementation of programmes benefiting both infected adolescents and their primary caregivers.
  • Full Text:
  • Date Issued: 2014
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