Hostage incident management : preparedness and response of international non-governmental organisations
- Authors: Lauvik. Kjell Erik
- Date: 2014
- Subjects: Non-governmental organizations -- Security measures , Hostage negotiations , Conflict management
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:8347 , http://hdl.handle.net/10948/d1020800
- Description: It is broadly accepted that there is a need for better security management and protocols for hostage incident management, there is currently a lack of basic empirical knowledge about the existing security management protocols with reference to existing policies, knowledge and the capability of International Non-Governmental Organisations (INGOs) to handle hostage incidents. Many INGOs have successfully managed high-profile hostage crises, but there is still a considerable level of uncertainty about the way these crises have been solved and the way their success can be seen in relation to other crises. This study aimed to understand how INGOs prepare themselves for hostage incidents, whether policies, procedures are in place, how they manage hostage situations, and also how INGO staff are trained and prepared. The methodology adopted for this study was qualitative and comprised of indepth interviews with sixteen INGOs and ten industry experts and a review of INGO documents, policies and plans. The study sheds light on some of the less talked-about aspects for INGO security management in general, as well as preparedness and responsibility towards their staff. The study suggests that while most organisations have a level of preparedness in place, enhancing each agency’s respective policies may assist the organisation in better management. The study also found that there is a higher use of ransom payment than expected, and that there is an increasing willingness to engage external expertise to assisting in managing a hostage crisis. The study makes several recommendations that may have policy implications, including pre-deployment hostile environment training, reviewing potential cooperation between INGOs and United Nations, and the use of external resources to assist in managing a hostage crisis. It also recommends a revision of existing negotiation models, as the current models are lacking in addressing protracted hostage cases. The establishment of an accurate database of incidents to allow for improved interpretation of trends and scope of hostage cases is also recommended.
- Full Text:
- Date Issued: 2014
- Authors: Lauvik. Kjell Erik
- Date: 2014
- Subjects: Non-governmental organizations -- Security measures , Hostage negotiations , Conflict management
- Language: English
- Type: Thesis , Doctoral , DPhil
- Identifier: vital:8347 , http://hdl.handle.net/10948/d1020800
- Description: It is broadly accepted that there is a need for better security management and protocols for hostage incident management, there is currently a lack of basic empirical knowledge about the existing security management protocols with reference to existing policies, knowledge and the capability of International Non-Governmental Organisations (INGOs) to handle hostage incidents. Many INGOs have successfully managed high-profile hostage crises, but there is still a considerable level of uncertainty about the way these crises have been solved and the way their success can be seen in relation to other crises. This study aimed to understand how INGOs prepare themselves for hostage incidents, whether policies, procedures are in place, how they manage hostage situations, and also how INGO staff are trained and prepared. The methodology adopted for this study was qualitative and comprised of indepth interviews with sixteen INGOs and ten industry experts and a review of INGO documents, policies and plans. The study sheds light on some of the less talked-about aspects for INGO security management in general, as well as preparedness and responsibility towards their staff. The study suggests that while most organisations have a level of preparedness in place, enhancing each agency’s respective policies may assist the organisation in better management. The study also found that there is a higher use of ransom payment than expected, and that there is an increasing willingness to engage external expertise to assisting in managing a hostage crisis. The study makes several recommendations that may have policy implications, including pre-deployment hostile environment training, reviewing potential cooperation between INGOs and United Nations, and the use of external resources to assist in managing a hostage crisis. It also recommends a revision of existing negotiation models, as the current models are lacking in addressing protracted hostage cases. The establishment of an accurate database of incidents to allow for improved interpretation of trends and scope of hostage cases is also recommended.
- Full Text:
- Date Issued: 2014
Housing backlogs in King Sabata Dalindyebo with specific attention to the housing problem at Bongweni Administrative Area in Mthatha
- Authors: Hlazo, Mthethuvumile
- Date: 2014
- Subjects: Public housing -- South Africa -- Mthatha , Municipal services -- South Africa -- Mthatha
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:9227 , http://hdl.handle.net/10948/d1020976
- Description: The study investigates problems affecting housing delivery in King Sabatha Dalindyebo Municipality Local Municipality. The housing development in this area is not up to standard. This situation is worse in rural areas where housing projects have been obstructed by lack of infrastructure and other essential services. This is a serious problem that this local municipality is facing; the most vulnerable are the poor, residing in these rural areas. The study has investigated this problem in King Sabatha Dalindyebo Municipality Local Municipality with specific attention to Bongweni Administrative Area in Mthatha. The investigation indicates that there are serious challenges facing housing.
- Full Text:
- Date Issued: 2014
- Authors: Hlazo, Mthethuvumile
- Date: 2014
- Subjects: Public housing -- South Africa -- Mthatha , Municipal services -- South Africa -- Mthatha
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:9227 , http://hdl.handle.net/10948/d1020976
- Description: The study investigates problems affecting housing delivery in King Sabatha Dalindyebo Municipality Local Municipality. The housing development in this area is not up to standard. This situation is worse in rural areas where housing projects have been obstructed by lack of infrastructure and other essential services. This is a serious problem that this local municipality is facing; the most vulnerable are the poor, residing in these rural areas. The study has investigated this problem in King Sabatha Dalindyebo Municipality Local Municipality with specific attention to Bongweni Administrative Area in Mthatha. The investigation indicates that there are serious challenges facing housing.
- Full Text:
- Date Issued: 2014
Housing challenges in Ekurhuleni Metropolitan Municipality: an exploration
- Authors: Marutlulle, Noah Kaliofas
- Date: 2014
- Language: English
- Type: Thesis , Doctoral , PhD (in Public Administration)
- Identifier: http://hdl.handle.net/10353/1234 , vital:26539
- Description: The housing delivery topic is frequently dredged to the surface of media focus by tragic phenomena such as the destruction of lives and properties by storms and fires and also at the hands of the police during demonstrations. This study which is exploratory in nature and adopted an interpretive research philosophy aimed at investigating the housing delivery challenges encountered by Ekurhuleni Metropolitan Municipality (EMM) with the objectives of illuminating the challenges, establishing the role EMM play in housing delivery and providing recommendations that would assist in ameliorating the challenges. It utilised a qualitative methodology involving indirect observation which included the use of questionnaires and interviews and a purposive sampling method where fifty eight participants comprising employees of EMM, Councillors, Community leaders and Community members were selected. The data were analysed in Microsoft word using thematic analysis and a pilot study was used to pre-test the objectivity, reliability and validity of the data and also to improve the success and effectiveness of the investigation. Overall, the results suggested the main causes of the housing delivery challenges to be predominantly a combination of population growth, the unavailability of land, corruption and government policies. The key findings that emerged for the amelioration of the challenges were the formulation of a coherent housing strategy, the need for the government to pursue housing policies that are comprehensive and effectively integrated with broader socioeconomic objectives and also the need for the government to be fully attuned to the necessity of reconfiguring housing policy as part of its mechanism for managing the economy. The general focus of the recommendations was on overcoming the housing delivery hurdles. In recommending future research, the existence of a vast amount of new knowledge that still need to be discovered on the housing delivery arena was established and avenues were indicated through which the knowledge can directly illuminate issues central to the theme of this study [housing delivery challenges]. The study will become a landmark and a standard reference in future studies wherein it will also serve as a barometer to test new and contentious ideas and theories encapsulated in issues surrounding the study.
- Full Text:
- Date Issued: 2014
- Authors: Marutlulle, Noah Kaliofas
- Date: 2014
- Language: English
- Type: Thesis , Doctoral , PhD (in Public Administration)
- Identifier: http://hdl.handle.net/10353/1234 , vital:26539
- Description: The housing delivery topic is frequently dredged to the surface of media focus by tragic phenomena such as the destruction of lives and properties by storms and fires and also at the hands of the police during demonstrations. This study which is exploratory in nature and adopted an interpretive research philosophy aimed at investigating the housing delivery challenges encountered by Ekurhuleni Metropolitan Municipality (EMM) with the objectives of illuminating the challenges, establishing the role EMM play in housing delivery and providing recommendations that would assist in ameliorating the challenges. It utilised a qualitative methodology involving indirect observation which included the use of questionnaires and interviews and a purposive sampling method where fifty eight participants comprising employees of EMM, Councillors, Community leaders and Community members were selected. The data were analysed in Microsoft word using thematic analysis and a pilot study was used to pre-test the objectivity, reliability and validity of the data and also to improve the success and effectiveness of the investigation. Overall, the results suggested the main causes of the housing delivery challenges to be predominantly a combination of population growth, the unavailability of land, corruption and government policies. The key findings that emerged for the amelioration of the challenges were the formulation of a coherent housing strategy, the need for the government to pursue housing policies that are comprehensive and effectively integrated with broader socioeconomic objectives and also the need for the government to be fully attuned to the necessity of reconfiguring housing policy as part of its mechanism for managing the economy. The general focus of the recommendations was on overcoming the housing delivery hurdles. In recommending future research, the existence of a vast amount of new knowledge that still need to be discovered on the housing delivery arena was established and avenues were indicated through which the knowledge can directly illuminate issues central to the theme of this study [housing delivery challenges]. The study will become a landmark and a standard reference in future studies wherein it will also serve as a barometer to test new and contentious ideas and theories encapsulated in issues surrounding the study.
- Full Text:
- Date Issued: 2014
How are the messages of the official grade ten sexuality education curriculum at a former model C girls' high school in South Africa mediated by student sexual cultures?
- Mthatyana, Andisiwe Tutula Zinzi
- Authors: Mthatyana, Andisiwe Tutula Zinzi
- Date: 2014
- Subjects: Sex instruction -- South Africa , Sex instruction -- Cross-cultural studies , Teenage pregnancy -- South Africa , High school girls -- Sexual behavior -- South Africa , Multicultural education -- South Africa , Model C schools (South Africa) , Girls' schools -- South Africa
- Language: English
- Type: Thesis , Masters , MSocSc
- Identifier: vital:2883 , http://hdl.handle.net/10962/d1013262
- Description: The increase in teenage pregnancy among school going learners is reported in the media as a crisis. Politicians and other stakeholders have also raised their views and concerns about pregnancy. In particular, these views and concerns perceive teenage pregnancy among school going learners as a cancer that needs a remedy because it has negative consequences for the learners, in particular the girl child. However, for all the sense of public crisis concerning sexuality and schooling, the voices of young people themselves regarding their own sexual subjectivity are seldom heard. This study focused on how girls in a former model C all girls high school negotiate and make sense of the meaning of the messages that they receive from the formal curriculum. The concept of student sexual cultures was employed in this study. Student sexual cultures are the informal groups that exist in the school and the girls take part in it. It is in these groups that the girls learn about sexuality and also make sense of their own gendered identities. This study employed ethnographic techniques of classroom observation coupled with in-depth interviews, focus groups and solicited narratives in order to understand how the participants experience and "take up" the messages they receive in the formal sexuality education component of the Life Orientation (LO) curriculum. The data was collected over a period of three months and was analysed using a directed content analysis. Four dominant themes emerged from the study. Firstly, the data reveals the school is a space of competing and conflicting discourses of sexuality and the learners are involved in a constant negotiation of the meanings of the messages. Secondly, the data shows the contested and confirmations of learners subjectivity. It shows that learners are regarded as sexual beings both in the formal and informal school cultures but there are limitations around one's sexual subjectivities. Thirdly, the data reveals that the school is a site in which a variety of femininities are reproduced, contested and struggled over. Femininities are constructed in the complex context of the school thus the school emerges as a site in which multiple femininities intersect with class, race and sexuality. Lastly, this study argues for the incorporation of the discourse of erotics in the formal curriculum which allows young people's voices to be heard. This approach (discourse of erotics) can be seen as a process of becoming, which focuses on possibilities of improving sexuality education as opposed to an imposed sexual model that is applied to young people and assumed to be the solution to young people's sexuality.
- Full Text:
- Date Issued: 2014
- Authors: Mthatyana, Andisiwe Tutula Zinzi
- Date: 2014
- Subjects: Sex instruction -- South Africa , Sex instruction -- Cross-cultural studies , Teenage pregnancy -- South Africa , High school girls -- Sexual behavior -- South Africa , Multicultural education -- South Africa , Model C schools (South Africa) , Girls' schools -- South Africa
- Language: English
- Type: Thesis , Masters , MSocSc
- Identifier: vital:2883 , http://hdl.handle.net/10962/d1013262
- Description: The increase in teenage pregnancy among school going learners is reported in the media as a crisis. Politicians and other stakeholders have also raised their views and concerns about pregnancy. In particular, these views and concerns perceive teenage pregnancy among school going learners as a cancer that needs a remedy because it has negative consequences for the learners, in particular the girl child. However, for all the sense of public crisis concerning sexuality and schooling, the voices of young people themselves regarding their own sexual subjectivity are seldom heard. This study focused on how girls in a former model C all girls high school negotiate and make sense of the meaning of the messages that they receive from the formal curriculum. The concept of student sexual cultures was employed in this study. Student sexual cultures are the informal groups that exist in the school and the girls take part in it. It is in these groups that the girls learn about sexuality and also make sense of their own gendered identities. This study employed ethnographic techniques of classroom observation coupled with in-depth interviews, focus groups and solicited narratives in order to understand how the participants experience and "take up" the messages they receive in the formal sexuality education component of the Life Orientation (LO) curriculum. The data was collected over a period of three months and was analysed using a directed content analysis. Four dominant themes emerged from the study. Firstly, the data reveals the school is a space of competing and conflicting discourses of sexuality and the learners are involved in a constant negotiation of the meanings of the messages. Secondly, the data shows the contested and confirmations of learners subjectivity. It shows that learners are regarded as sexual beings both in the formal and informal school cultures but there are limitations around one's sexual subjectivities. Thirdly, the data reveals that the school is a site in which a variety of femininities are reproduced, contested and struggled over. Femininities are constructed in the complex context of the school thus the school emerges as a site in which multiple femininities intersect with class, race and sexuality. Lastly, this study argues for the incorporation of the discourse of erotics in the formal curriculum which allows young people's voices to be heard. This approach (discourse of erotics) can be seen as a process of becoming, which focuses on possibilities of improving sexuality education as opposed to an imposed sexual model that is applied to young people and assumed to be the solution to young people's sexuality.
- Full Text:
- Date Issued: 2014
How does security limit the right to protest? : a study examining the securitised response to protest in South Africa
- Authors: Royeppen, Andrea Leigh
- Date: 2014
- Subjects: Protest movements -- South Africa , Political violence -- South Africa , South Africa -- Politics and government -- 21st century , Civil rights -- South Africa , Police power -- South Africa , Abuse of administrative power -- South Africa , Police -- Complaints against -- South Africa , Right to strike -- South Africa , Democracy -- South Africa , Political leadership -- South Africa -- 21st century , Political participation -- South Africa , African National Congress , South African Police Service
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2878 , http://hdl.handle.net/10962/d1013071
- Description: In South Africa, the right to protest is under constant threat as a result of the state response. Increasing cases of forceful policing and sometimes unlawful procedural prohibitions of protest attest to this. This study aims to firstly describe this situation through securitisation theory, essentially arguing that South Africa has become a securitised state. It also aims to understand how this is sustained by the state and why the state needs to use a securitised response to maintain power. Interviews were conducted with members of different communities and organisations. Their responses helped to illustrate the frustration of the right to protest or brutal policing during a protest. This provided primary evidence to support the claims of the study. The research shows that claims to protest are being delegitimised under the guise of security as protestors are being constructed as threats to the state. This is further substantiated by looking at how the reorganisation and remililtarisation of the South African Police perpetuates the criminalisation of protestors which necessitates a forceful response from the state. Furthermore, it shows that there is a distinct relationship between the prohibition of protest and the recent increase in ‘violent’ protests which legitimate forceful policing thereby creating a state sustained cycle of violence. The larger implication of this treatment is that these protestors are treated as non- citizens who are definitively excluded from participating in governance. In understanding why this is taking place, it is clear that a securtitised response is an attempt to maintain power by dispelling any threats to power, a response which is seen to have a long history in the African National Congress (ANC) when examining the politics of the ANC during exile. Maintaining power in this way distracts from the larger agenda of the state, which this thesis argues, is to mask the unraveling of the ANC’s hegemony and inability to maintain national unity. In other words, the increasing dissatisfaction of some of the citizenry which has manifested through protest greatly undermines the legitimacy of the government to provide for its people.
- Full Text:
- Date Issued: 2014
- Authors: Royeppen, Andrea Leigh
- Date: 2014
- Subjects: Protest movements -- South Africa , Political violence -- South Africa , South Africa -- Politics and government -- 21st century , Civil rights -- South Africa , Police power -- South Africa , Abuse of administrative power -- South Africa , Police -- Complaints against -- South Africa , Right to strike -- South Africa , Democracy -- South Africa , Political leadership -- South Africa -- 21st century , Political participation -- South Africa , African National Congress , South African Police Service
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2878 , http://hdl.handle.net/10962/d1013071
- Description: In South Africa, the right to protest is under constant threat as a result of the state response. Increasing cases of forceful policing and sometimes unlawful procedural prohibitions of protest attest to this. This study aims to firstly describe this situation through securitisation theory, essentially arguing that South Africa has become a securitised state. It also aims to understand how this is sustained by the state and why the state needs to use a securitised response to maintain power. Interviews were conducted with members of different communities and organisations. Their responses helped to illustrate the frustration of the right to protest or brutal policing during a protest. This provided primary evidence to support the claims of the study. The research shows that claims to protest are being delegitimised under the guise of security as protestors are being constructed as threats to the state. This is further substantiated by looking at how the reorganisation and remililtarisation of the South African Police perpetuates the criminalisation of protestors which necessitates a forceful response from the state. Furthermore, it shows that there is a distinct relationship between the prohibition of protest and the recent increase in ‘violent’ protests which legitimate forceful policing thereby creating a state sustained cycle of violence. The larger implication of this treatment is that these protestors are treated as non- citizens who are definitively excluded from participating in governance. In understanding why this is taking place, it is clear that a securtitised response is an attempt to maintain power by dispelling any threats to power, a response which is seen to have a long history in the African National Congress (ANC) when examining the politics of the ANC during exile. Maintaining power in this way distracts from the larger agenda of the state, which this thesis argues, is to mask the unraveling of the ANC’s hegemony and inability to maintain national unity. In other words, the increasing dissatisfaction of some of the citizenry which has manifested through protest greatly undermines the legitimacy of the government to provide for its people.
- Full Text:
- Date Issued: 2014
How organisational behaviour is influenced in an acquired firm
- Authors: Mesani, Anele
- Date: 2014
- Subjects: Organizational effectiveness Organizational behaviour
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/9925 , vital:26633
- Description: In the current global market, companies are forming partnerships with other organisations with the aim of sustaining their competitive advantage. Most organisations participate in transactions such as mergers, acquisitions and joint ventures in order to maintain their competitive advantage (Sorge, 2002). South Africa forms part of the global market and is not immune towards the stipulated growth strategies. It is believed that South Africa has one of the highest unemployment rate internationally (Human Capital Mangement, 2005). There are various reasons that have contributed towards this perception; factors such as structural changes in the labour market have been highlighted as one of the factors that have contributed to the high unemployment rate (HCM, 2005). It is further said that when mergers and acquisitions occurs, structural changes are bound to occur (Greeve, 2008). Despite the constant merger activities that are occurring in various industries, research has shown that most mergers add no value or reduce shareholders value for the acquiring firm (Kusewitt, 2007). The primary objective of this research is to investigate how organisational behaviour is influenced in an acquired firm.There seems to be high level of uncertainity, trust and communication breakdown amoung employees of Kansai Plascon. This research will investigate whether this perception is related to the acquisition transaction that has occurred. The study will collect its primary data using a set of questionnaires that will be distributed to the employees of Kansai Plascon. The data will be analysed and interpreted.
- Full Text:
- Date Issued: 2014
- Authors: Mesani, Anele
- Date: 2014
- Subjects: Organizational effectiveness Organizational behaviour
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/9925 , vital:26633
- Description: In the current global market, companies are forming partnerships with other organisations with the aim of sustaining their competitive advantage. Most organisations participate in transactions such as mergers, acquisitions and joint ventures in order to maintain their competitive advantage (Sorge, 2002). South Africa forms part of the global market and is not immune towards the stipulated growth strategies. It is believed that South Africa has one of the highest unemployment rate internationally (Human Capital Mangement, 2005). There are various reasons that have contributed towards this perception; factors such as structural changes in the labour market have been highlighted as one of the factors that have contributed to the high unemployment rate (HCM, 2005). It is further said that when mergers and acquisitions occurs, structural changes are bound to occur (Greeve, 2008). Despite the constant merger activities that are occurring in various industries, research has shown that most mergers add no value or reduce shareholders value for the acquiring firm (Kusewitt, 2007). The primary objective of this research is to investigate how organisational behaviour is influenced in an acquired firm.There seems to be high level of uncertainity, trust and communication breakdown amoung employees of Kansai Plascon. This research will investigate whether this perception is related to the acquisition transaction that has occurred. The study will collect its primary data using a set of questionnaires that will be distributed to the employees of Kansai Plascon. The data will be analysed and interpreted.
- Full Text:
- Date Issued: 2014
How technology can be applied to support change management best practices in the South African automotive industry
- Authors: Vorster, Malcolm William
- Date: 2014
- Subjects: Organizational change -- South Africa , Automobile industry and trade -- Information technology -- South Africa
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/4265 , vital:20576
- Description: Organisations within the South African automotive industry (SAAI) are always seeking innovative ways to remain globally competitive. In order to keep costs as low as possible, existing facilities are often adapted to either cater for a wider variety of products or, alternatively, to produce larger volumes than they were originally designed to produce. In every instance, some form of change needs to take place. When the change management processes are defined within the system, quick wins need to be identified. These quick wins may include the elimination of non-valueadding time that is spent moving paper work between offices or ensuring the correct checks are in place to prevent the process from moving to the next step until all the requirements for the current steps have been completed. Technology has been identified as a suitable support mechanism that would be able to integrate into the complex system that comprises processes to be followed in an order that can be predefined. Every organisation faces its own unique challenges when technology is introduced. These can include a lack of computer literacy and the unwillingness to accept that change can in fact benefit the organisation. Getting the users to take ownership of the new systems through comprehensive training initiatives will be shown to be the most effective manner in which to ensure that the systems are effective and used to their full potential. This treatise will investigate the change management systems currently being used in the SAAI and compare the most important factors against the perceived best practices of the resources that are involved in change management. Organisations that operate at various levels within the SAAI will be researched. A literature review of the best practices in change management systems combined with an investigation into how technology can assist in supporting these best practices will be conducted. The findings will then be summarised and recommendations based on the collected data and information will be formulated and put forward.
- Full Text:
- Date Issued: 2014
- Authors: Vorster, Malcolm William
- Date: 2014
- Subjects: Organizational change -- South Africa , Automobile industry and trade -- Information technology -- South Africa
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: http://hdl.handle.net/10948/4265 , vital:20576
- Description: Organisations within the South African automotive industry (SAAI) are always seeking innovative ways to remain globally competitive. In order to keep costs as low as possible, existing facilities are often adapted to either cater for a wider variety of products or, alternatively, to produce larger volumes than they were originally designed to produce. In every instance, some form of change needs to take place. When the change management processes are defined within the system, quick wins need to be identified. These quick wins may include the elimination of non-valueadding time that is spent moving paper work between offices or ensuring the correct checks are in place to prevent the process from moving to the next step until all the requirements for the current steps have been completed. Technology has been identified as a suitable support mechanism that would be able to integrate into the complex system that comprises processes to be followed in an order that can be predefined. Every organisation faces its own unique challenges when technology is introduced. These can include a lack of computer literacy and the unwillingness to accept that change can in fact benefit the organisation. Getting the users to take ownership of the new systems through comprehensive training initiatives will be shown to be the most effective manner in which to ensure that the systems are effective and used to their full potential. This treatise will investigate the change management systems currently being used in the SAAI and compare the most important factors against the perceived best practices of the resources that are involved in change management. Organisations that operate at various levels within the SAAI will be researched. A literature review of the best practices in change management systems combined with an investigation into how technology can assist in supporting these best practices will be conducted. The findings will then be summarised and recommendations based on the collected data and information will be formulated and put forward.
- Full Text:
- Date Issued: 2014
How to establish a bioinformatics postgraduate degree programme—a case study from South Africa
- Machanick, Philip, Tastan Bishop, Özlem
- Authors: Machanick, Philip , Tastan Bishop, Özlem
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/124641 , vital:35641 , https://doi.10.1093/bib/bbu014
- Description: The Research Unit in Bioinformatics at Rhodes University (RUBi), South Africa, offers a Masters of Science in Bioinformatics.Growing demand for bioinformatics qualifications results in applications from across Africa.Courses aim to bridge gaps in the diverse backgrounds of students who range from biologists with no prior computing exposure to computer scientists with no biology background. The programme is evenly split between coursework and research, with diverse modules from a range of departments coveringmathematics, statistics, computer science and biology, with emphasis on application to bioinformatics research. The early focus on research helps bring students up to speed with working as a researcher. We measure success of the programme by the high rate of subsequent entry to PhD study: 10 of 14 students who completed in the years 2011-2013.
- Full Text:
- Date Issued: 2014
- Authors: Machanick, Philip , Tastan Bishop, Özlem
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/124641 , vital:35641 , https://doi.10.1093/bib/bbu014
- Description: The Research Unit in Bioinformatics at Rhodes University (RUBi), South Africa, offers a Masters of Science in Bioinformatics.Growing demand for bioinformatics qualifications results in applications from across Africa.Courses aim to bridge gaps in the diverse backgrounds of students who range from biologists with no prior computing exposure to computer scientists with no biology background. The programme is evenly split between coursework and research, with diverse modules from a range of departments coveringmathematics, statistics, computer science and biology, with emphasis on application to bioinformatics research. The early focus on research helps bring students up to speed with working as a researcher. We measure success of the programme by the high rate of subsequent entry to PhD study: 10 of 14 students who completed in the years 2011-2013.
- Full Text:
- Date Issued: 2014
How to open the door
- Authors: Beyers, Marike
- Date: 2014
- Subjects: Creative writing (Higher education) -- Research -- South Africa Creative writing -- Poetry South African poetry (English) -- Study and teaching (Higher) South African poetry (English) -- 21st century English language -- Writing
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:5969 , http://hdl.handle.net/10962/d1011502
- Description: A collection of mostly lyrical poems. The poems explore moments of experience and thought relating to longing and belonging, in terms of relations, memory and place. The poems are mostly short and intense. Silence and implied meanings are often as important as what is said; shadows are evoked to recall substance. Though short, the poems are not tightly closed – on the contrary, meanings proliferate in the process of exploration
- Full Text:
- Date Issued: 2014
- Authors: Beyers, Marike
- Date: 2014
- Subjects: Creative writing (Higher education) -- Research -- South Africa Creative writing -- Poetry South African poetry (English) -- Study and teaching (Higher) South African poetry (English) -- 21st century English language -- Writing
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:5969 , http://hdl.handle.net/10962/d1011502
- Description: A collection of mostly lyrical poems. The poems explore moments of experience and thought relating to longing and belonging, in terms of relations, memory and place. The poems are mostly short and intense. Silence and implied meanings are often as important as what is said; shadows are evoked to recall substance. Though short, the poems are not tightly closed – on the contrary, meanings proliferate in the process of exploration
- Full Text:
- Date Issued: 2014
HPLC method for simultaneous analysis of ranitidine and metronidazole in dosage forms
- King'ori, Loti D, Walker, Roderick B
- Authors: King'ori, Loti D , Walker, Roderick B
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/184790 , vital:44272 , xlink:href="https://doi.org/10.14233/ajchem.2014.15432"
- Description: A simple, rapid, precise and accurate stability indicating HPLC method for the simultaneous analysis of metronidazole and ranitidine in dosage forms has been developed and validated. Calibration curves for metronidazole and ranitidine exhibited linearity (R2 = 0.9995 for both compounds) over the concentration ranges investigated. The method was sensitive, selective and accurate for both compounds. Both drugs were found to be stable following acid hydrolysis studies. However, following alkali hydrolysis degradation of both compounds was observed. Furthermore metronidazole appeared to be stable following oxidative studies however ranitidine underwent complete degradation under these conditions. Both drugs were well resolved from the degradation products. The stability indicating chromatographic method has the necessary precision and accuracy for the simultaneous analysis of metronidazole and ranitidine in dosage forms.
- Full Text:
- Date Issued: 2014
- Authors: King'ori, Loti D , Walker, Roderick B
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/184790 , vital:44272 , xlink:href="https://doi.org/10.14233/ajchem.2014.15432"
- Description: A simple, rapid, precise and accurate stability indicating HPLC method for the simultaneous analysis of metronidazole and ranitidine in dosage forms has been developed and validated. Calibration curves for metronidazole and ranitidine exhibited linearity (R2 = 0.9995 for both compounds) over the concentration ranges investigated. The method was sensitive, selective and accurate for both compounds. Both drugs were found to be stable following acid hydrolysis studies. However, following alkali hydrolysis degradation of both compounds was observed. Furthermore metronidazole appeared to be stable following oxidative studies however ranitidine underwent complete degradation under these conditions. Both drugs were well resolved from the degradation products. The stability indicating chromatographic method has the necessary precision and accuracy for the simultaneous analysis of metronidazole and ranitidine in dosage forms.
- Full Text:
- Date Issued: 2014
Hsp90 binds directly to fibronectin (FN) and inhibition reduces the extracellular fibronectin matrix in breast cancer cells
- Hunter, Morgan C, O’Hagan, Kyle L, Kenyon, Amy, Dhanani, Karim C H, Prinsloo, Earl, Edkins, Adrienne L
- Authors: Hunter, Morgan C , O’Hagan, Kyle L , Kenyon, Amy , Dhanani, Karim C H , Prinsloo, Earl , Edkins, Adrienne L
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/431143 , vital:72748 , xlink:href="https://doi.org/10.1371/journal.pone.0086842"
- Description: Heat shock protein 90 (Hsp90) has been identified in the extracellular space and has been shown to chaperone a finite number of extracellular proteins involved in cell migration and invasion. We used chemical cross-linking and immunoprecipitation followed by tandem mass spectrometry (MS/MS) to isolate a complex containing Hsp90 and the matrix protein fibronectin (FN) from breast cancer cells. Further analysis showed direct binding of Hsp90 to FN using an in vitro co-immunoprecipitation assay, a solid phase binding assay and surface plasmon resonance (SPR) spectroscopy. Confocal microscopy showed regions of co-localisation of Hsp90 and FN in breast cancer cell lines. Exogenous Hsp90β was shown to increase the formation of extracellular FN matrix in the Hs578T cell line, whilst knockdown or inhibition of Hsp90 led to a reduction in the levels of both soluble and insoluble FN and could be partially rescued by addition of exogenous Hsp90β. Treatment of cells with novobiocin led to internalization of FN into vesicles that were positive for the presence of the lysosomal marker, LAMP-1. Taken together, the direct interaction between FN and Hsp90, as well as the decreased levels of both soluble and insoluble FN upon Hsp90 inhibition or knockdown, suggested that FN may be a new client protein for Hsp90 and that Hsp90 was involved in FN matrix assembly and/or stability. The identification of FN as a putative client protein of Hsp90 suggests a role for Hsp90 in FN matrix stability, which is important for a number of fundamental cellular processes including embryogenesis, wound healing, cell migration and metastasis.
- Full Text:
- Date Issued: 2014
- Authors: Hunter, Morgan C , O’Hagan, Kyle L , Kenyon, Amy , Dhanani, Karim C H , Prinsloo, Earl , Edkins, Adrienne L
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/431143 , vital:72748 , xlink:href="https://doi.org/10.1371/journal.pone.0086842"
- Description: Heat shock protein 90 (Hsp90) has been identified in the extracellular space and has been shown to chaperone a finite number of extracellular proteins involved in cell migration and invasion. We used chemical cross-linking and immunoprecipitation followed by tandem mass spectrometry (MS/MS) to isolate a complex containing Hsp90 and the matrix protein fibronectin (FN) from breast cancer cells. Further analysis showed direct binding of Hsp90 to FN using an in vitro co-immunoprecipitation assay, a solid phase binding assay and surface plasmon resonance (SPR) spectroscopy. Confocal microscopy showed regions of co-localisation of Hsp90 and FN in breast cancer cell lines. Exogenous Hsp90β was shown to increase the formation of extracellular FN matrix in the Hs578T cell line, whilst knockdown or inhibition of Hsp90 led to a reduction in the levels of both soluble and insoluble FN and could be partially rescued by addition of exogenous Hsp90β. Treatment of cells with novobiocin led to internalization of FN into vesicles that were positive for the presence of the lysosomal marker, LAMP-1. Taken together, the direct interaction between FN and Hsp90, as well as the decreased levels of both soluble and insoluble FN upon Hsp90 inhibition or knockdown, suggested that FN may be a new client protein for Hsp90 and that Hsp90 was involved in FN matrix assembly and/or stability. The identification of FN as a putative client protein of Hsp90 suggests a role for Hsp90 in FN matrix stability, which is important for a number of fundamental cellular processes including embryogenesis, wound healing, cell migration and metastasis.
- Full Text:
- Date Issued: 2014
Hsp90 binds directly to fibronectin (FN) and inhibition reduces the extracellular fibronectin matrix in breast cancer cells:
- Hunter, Morgan C, O’Hagan, Kyle L, Kenyon, Amy, Dhanani, Karim C H, Prinsloo, Earl, Edkins, Adrienne L
- Authors: Hunter, Morgan C , O’Hagan, Kyle L , Kenyon, Amy , Dhanani, Karim C H , Prinsloo, Earl , Edkins, Adrienne L
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/164841 , vital:41177 , DOI: 10.1371/journal.pone.0086842
- Description: Heat shock protein 90 (Hsp90) has been identified in the extracellular space and has been shown to chaperone a finite number of extracellular proteins involved in cell migration and invasion. We used chemical cross-linking and immunoprecipitation followed by tandem mass spectrometry (MS/MS) to isolate a complex containing Hsp90 and the matrix protein fibronectin (FN) from breast cancer cells.
- Full Text:
- Date Issued: 2014
- Authors: Hunter, Morgan C , O’Hagan, Kyle L , Kenyon, Amy , Dhanani, Karim C H , Prinsloo, Earl , Edkins, Adrienne L
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/164841 , vital:41177 , DOI: 10.1371/journal.pone.0086842
- Description: Heat shock protein 90 (Hsp90) has been identified in the extracellular space and has been shown to chaperone a finite number of extracellular proteins involved in cell migration and invasion. We used chemical cross-linking and immunoprecipitation followed by tandem mass spectrometry (MS/MS) to isolate a complex containing Hsp90 and the matrix protein fibronectin (FN) from breast cancer cells.
- Full Text:
- Date Issued: 2014
Human perception of the measurement of a network attack taxonomy in near real-time
- Van Heerden, Renier, Malan, Mercia M, Mouton, Francois, Irwin, Barry V W
- Authors: Van Heerden, Renier , Malan, Mercia M , Mouton, Francois , Irwin, Barry V W
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/429924 , vital:72652 , https://doi.org/10.1007/978-3-662-44208-1_23
- Description: This paper investigates how the measurement of a network attack taxonomy can be related to human perception. Network attacks do not have a time limitation, but the earlier its detected, the more damage can be prevented and the more preventative actions can be taken. This paper evaluate how elements of network attacks can be measured in near real-time(60 seconds). The taxonomy we use was developed by van Heerden et al (2012) with over 100 classes. These classes present the attack and defenders point of view. The degree to which each class can be quantified or measured is determined by investigating the accuracy of various assessment methods. We classify each class as either defined, high, low or not quantifiable. For example, it may not be possible to determine the instigator of an attack (Aggressor), but only that the attack has been launched by a Hacker (Actor). Some classes can only be quantified with a low confidence or not at all in a sort (near real-time) time. The IP address of an attack can easily be faked thus reducing the confidence in the information obtained from it, and thus determining the origin of an attack with a low confidence. This determination itself is subjective. All the evaluations of the classes in this paper is subjective, but due to the very basic grouping (High, Low or Not Quantifiable) a subjective value can be used. The complexity of the taxonomy can be significantly reduced if classes with only a high perceptive accuracy is used.
- Full Text:
- Date Issued: 2014
- Authors: Van Heerden, Renier , Malan, Mercia M , Mouton, Francois , Irwin, Barry V W
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/429924 , vital:72652 , https://doi.org/10.1007/978-3-662-44208-1_23
- Description: This paper investigates how the measurement of a network attack taxonomy can be related to human perception. Network attacks do not have a time limitation, but the earlier its detected, the more damage can be prevented and the more preventative actions can be taken. This paper evaluate how elements of network attacks can be measured in near real-time(60 seconds). The taxonomy we use was developed by van Heerden et al (2012) with over 100 classes. These classes present the attack and defenders point of view. The degree to which each class can be quantified or measured is determined by investigating the accuracy of various assessment methods. We classify each class as either defined, high, low or not quantifiable. For example, it may not be possible to determine the instigator of an attack (Aggressor), but only that the attack has been launched by a Hacker (Actor). Some classes can only be quantified with a low confidence or not at all in a sort (near real-time) time. The IP address of an attack can easily be faked thus reducing the confidence in the information obtained from it, and thus determining the origin of an attack with a low confidence. This determination itself is subjective. All the evaluations of the classes in this paper is subjective, but due to the very basic grouping (High, Low or Not Quantifiable) a subjective value can be used. The complexity of the taxonomy can be significantly reduced if classes with only a high perceptive accuracy is used.
- Full Text:
- Date Issued: 2014
Hyperbenthic and pelagic predators regulate alternate key planktonic copepods in shallow temperate estuaries
- Wasserman, Ryan J, Vink, Tim J F, Kramer, Rachel, Froneman, P William
- Authors: Wasserman, Ryan J , Vink, Tim J F , Kramer, Rachel , Froneman, P William
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/68224 , vital:29220 , https://doi.org/10.1071/MF13233
- Description: Publisher version , Although predation has been identified as an important community driver, the role of predator diversity in structuring estuarine zooplankton has not been assessed. As such, we investigated the effects of two different zooplanktivorous fish species on the estuarine zooplankton community during a 12-day mesocosm study. Three experimental treatments were established, whereby natural zooplankton communities were subject to either (1) no predatory pressure, (2) predation by a pelagic predator (Monodactylus falciformis) or (3) predation by a hyper-benthic predator (Glossogobius callidus). The pelagic feeding M. falciformis fed largely on the numerically dominant mid-water copepod species, Paracartia longipatella. In contrast, the hyper-benthic fish had a greater predatory impact on the less numerically dominant copepod, Pseudodiaptomus hessei, which demonstrates strong diel vertical migration. Variations in prey-population regulation are ascribed to the distinct behavioural differences of the predators, and mediated by the differences in behaviour of the copepod species.
- Full Text: false
- Date Issued: 2014
- Authors: Wasserman, Ryan J , Vink, Tim J F , Kramer, Rachel , Froneman, P William
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/68224 , vital:29220 , https://doi.org/10.1071/MF13233
- Description: Publisher version , Although predation has been identified as an important community driver, the role of predator diversity in structuring estuarine zooplankton has not been assessed. As such, we investigated the effects of two different zooplanktivorous fish species on the estuarine zooplankton community during a 12-day mesocosm study. Three experimental treatments were established, whereby natural zooplankton communities were subject to either (1) no predatory pressure, (2) predation by a pelagic predator (Monodactylus falciformis) or (3) predation by a hyper-benthic predator (Glossogobius callidus). The pelagic feeding M. falciformis fed largely on the numerically dominant mid-water copepod species, Paracartia longipatella. In contrast, the hyper-benthic fish had a greater predatory impact on the less numerically dominant copepod, Pseudodiaptomus hessei, which demonstrates strong diel vertical migration. Variations in prey-population regulation are ascribed to the distinct behavioural differences of the predators, and mediated by the differences in behaviour of the copepod species.
- Full Text: false
- Date Issued: 2014
Ideas and power: shaping monetary policy in South Africa 1919-1936
- Authors: Bordiss, Bradley John
- Date: 2014
- Subjects: Monetary policy -- South Africa -- 1919-1936 Economic development -- South Africa -- 1919-1936 Economics -- South Africa -- History Economics -- Philosophy South Africa -- Economic policy -- 1919-1936 South Africa -- Foreign economic relations -- 1919-1936 Great Britain -- Foreign economic relations -- 1919-1936 Great Britain -- Economic policy -- 1918-1945
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:1084 , http://hdl.handle.net/10962/d1011605
- Description: In the concluding paragraphs of Keynes’ General Theory, Keynes suggests that vested interests (power) may dominate in the short term, but that “sooner or later, it is ideas, not vested interests, which are dangerous for good or evil” (Keynes; 1936:384). This dissertation seeks to establish whether this is so, and to what extent, in the period 1919 to 1936, insofar as the shaping of monetary policy was concerned. The context that South Africa found itself in at the time was one in which Britain, the colonising power, was in economic decline. Britain’s real economy had lost its lead in the world in the late 1800s, and by our period, 1919 – 1936, she was now struggling to maintain her dominance of the world’s financial economy. South African gold flows to London, and a South African monetary policy supportive of British monetary policy, became more important than ever to Britain. On the back of its ascendant real economy, the United States of America was fast developing its financial sector as a rival to that centered on London. In the broader monetary policy world, the orthodox monetary regime of the Gold Standard, which had worked so well in the period from 1875 to 1914, was firstly difficult to reestablish, and once established, difficult to maintain. Opinion on what should be done was divided between the majority who favoured a return to the orthodoxy, and a much smaller group, including John Maynard Keynes, who argued that the Gold Standard should no longer be the preferred monetary system. In South Africa, our period starts 17 years after the Second Boer War. Afrikaner nationalists intent on establishing independence from Britain, competed with those, including Jan Christiaan Smuts, who believed that tying our policy up with that of the British Empire was the best for South Africa. It is in this context that a naturalised Briton, which the research shows was a loyal servant of the London power elite, was appointed by the Empire-friendly Smuts government to advise the South African government on monetary policy, the setting up of the South African Reserve Bank, the appointment of its first Governor and other matters in the period up until the fall of this government in 1924. It is also in this context that an American ‘Currency Doctor’ and Professor of Economics at Princeton University, which the research shows was intimately connected with the American government and Benjamin Strong at the Federal Reserve, was appointed by the Pact government later in 1924, and who was anxious to throw off the yoke of British control. The theoretical paradigm of this study is that developed by John Maynard Keynes and after him by the post-Keynesian economists, particularly Basil Moore and Hyman P. Minsky. Instead of considering the theory chronologically, book by book, the theory section deals with the subject matter in the themes which came up in the monetary policy debates of the time, looking at all the theoretical literature that applied to these various themes. Aside from the correction of errors of emphasis and errors of fact dealt with in chapter two, chapter five of the dissertation is where most of the original research is reflected. This is the section which deals in depth with the experts that advised the South Africans at the time, how they came to be appointed, whose interests they served, what theories they used in support of their positions, and what was the decision-making process; from their appointment, until their reports were drafted into the law of the Union of South Africa. While Ally’s work (1994) is accepted as the principal work on the influence of the Bank of England, and Britain’s control of South African gold on South African gold and monetary policy, this dissertation claims legitimacy based on a much closer look at the motives and vested interests of the experts advising the South African government at the time. By the end of this chapter, I believe we are better placed to understand and analyse the relative influence of ideas and power on monetary policy in the period 1919 – 1936.
- Full Text:
- Date Issued: 2014
- Authors: Bordiss, Bradley John
- Date: 2014
- Subjects: Monetary policy -- South Africa -- 1919-1936 Economic development -- South Africa -- 1919-1936 Economics -- South Africa -- History Economics -- Philosophy South Africa -- Economic policy -- 1919-1936 South Africa -- Foreign economic relations -- 1919-1936 Great Britain -- Foreign economic relations -- 1919-1936 Great Britain -- Economic policy -- 1918-1945
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:1084 , http://hdl.handle.net/10962/d1011605
- Description: In the concluding paragraphs of Keynes’ General Theory, Keynes suggests that vested interests (power) may dominate in the short term, but that “sooner or later, it is ideas, not vested interests, which are dangerous for good or evil” (Keynes; 1936:384). This dissertation seeks to establish whether this is so, and to what extent, in the period 1919 to 1936, insofar as the shaping of monetary policy was concerned. The context that South Africa found itself in at the time was one in which Britain, the colonising power, was in economic decline. Britain’s real economy had lost its lead in the world in the late 1800s, and by our period, 1919 – 1936, she was now struggling to maintain her dominance of the world’s financial economy. South African gold flows to London, and a South African monetary policy supportive of British monetary policy, became more important than ever to Britain. On the back of its ascendant real economy, the United States of America was fast developing its financial sector as a rival to that centered on London. In the broader monetary policy world, the orthodox monetary regime of the Gold Standard, which had worked so well in the period from 1875 to 1914, was firstly difficult to reestablish, and once established, difficult to maintain. Opinion on what should be done was divided between the majority who favoured a return to the orthodoxy, and a much smaller group, including John Maynard Keynes, who argued that the Gold Standard should no longer be the preferred monetary system. In South Africa, our period starts 17 years after the Second Boer War. Afrikaner nationalists intent on establishing independence from Britain, competed with those, including Jan Christiaan Smuts, who believed that tying our policy up with that of the British Empire was the best for South Africa. It is in this context that a naturalised Briton, which the research shows was a loyal servant of the London power elite, was appointed by the Empire-friendly Smuts government to advise the South African government on monetary policy, the setting up of the South African Reserve Bank, the appointment of its first Governor and other matters in the period up until the fall of this government in 1924. It is also in this context that an American ‘Currency Doctor’ and Professor of Economics at Princeton University, which the research shows was intimately connected with the American government and Benjamin Strong at the Federal Reserve, was appointed by the Pact government later in 1924, and who was anxious to throw off the yoke of British control. The theoretical paradigm of this study is that developed by John Maynard Keynes and after him by the post-Keynesian economists, particularly Basil Moore and Hyman P. Minsky. Instead of considering the theory chronologically, book by book, the theory section deals with the subject matter in the themes which came up in the monetary policy debates of the time, looking at all the theoretical literature that applied to these various themes. Aside from the correction of errors of emphasis and errors of fact dealt with in chapter two, chapter five of the dissertation is where most of the original research is reflected. This is the section which deals in depth with the experts that advised the South Africans at the time, how they came to be appointed, whose interests they served, what theories they used in support of their positions, and what was the decision-making process; from their appointment, until their reports were drafted into the law of the Union of South Africa. While Ally’s work (1994) is accepted as the principal work on the influence of the Bank of England, and Britain’s control of South African gold on South African gold and monetary policy, this dissertation claims legitimacy based on a much closer look at the motives and vested interests of the experts advising the South African government at the time. By the end of this chapter, I believe we are better placed to understand and analyse the relative influence of ideas and power on monetary policy in the period 1919 – 1936.
- Full Text:
- Date Issued: 2014
Identification of membrane biomakers for colorectal cancer using an in-solico and molecular approach
- Authors: Van Vuuren, Larry Peter
- Date: 2014
- Subjects: Cancer -- Research , Colon (Anatomy) -- Cancer , Rectum -- Cancer
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/47970 , vital:40457
- Description: The aim of this study was to identify membrane biomarkers for colorectal cancer using an insilico and molecular approach. Colorectal cancer (CRC) globally accounts for more than half a million deaths. In South Africa alone, approximately one in 97 men is at risk of getting CRC; and for women, it is one in 162. Novel and non-invasive diagnostic tools, such as biomarkers, are needed for early CRC detection. In order to reduce the fatality rate in this disease, Biomarkers are used as indicators of a biological state; and they are measurable in biological media. They can be used to distinguish between a diseased state and a normal state, thus aiding diagnostics, response to specific therapies, and screening for an early diagnosis. A gene list of potential CRC biomarkers was generated by mining two gene databases, namely: Oncomine and Gene Expression Atlas. A total of 44 candidate genes were identified, based on their location on the cell surface, using the Database for Annotation, Visualisation and Integrated Discovery. These 44 genes were then subjected to an in-depth literature mining. The literature search parameters in PubMed, PubMed Central, Google Scholar and Science direct revealed publications showing that 23 genes were validated, while 21 genes were not validated. Nineteen genes were selected for gene validation in human colorectal cancer and healthy tissue of twelve patients. Total RNA was extracted from 12 colorectal cancer and 12 healthy tissue samples. The RNA was then quantified and reverse-transcribed into cDNA for gene expression analysis. The qPCR running conditions were optimized, by running a melting curve, in order to determine the optimum annealing temperatures. Primarily melt curves were run for nineteen of these twenty-one genes. Melt-curve analysis showed that nine genes were poor candidates for further validation studies; and therefore, only ten genes, namely: AGTRAP, ANKRD46, BACE2, CFB, CIAO1, NOMO3, PTDSS1, SLC5A6, TNFRSF12A and ZDHHC9 were validated by qPCR in human resected colorectal carcinoma and the normal tissues of twelve patients. The qPCR results showed that ZDHHC9 and the SLC5A6 genes were the only two statistically significant ones; and they were found to be down-regulated in human colorectal cancer vs healthy tissue samples.
- Full Text:
- Date Issued: 2014
Identification of membrane biomakers for colorectal cancer using an in-solico and molecular approach
- Authors: Van Vuuren, Larry Peter
- Date: 2014
- Subjects: Cancer -- Research , Colon (Anatomy) -- Cancer , Rectum -- Cancer
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10948/47970 , vital:40457
- Description: The aim of this study was to identify membrane biomarkers for colorectal cancer using an insilico and molecular approach. Colorectal cancer (CRC) globally accounts for more than half a million deaths. In South Africa alone, approximately one in 97 men is at risk of getting CRC; and for women, it is one in 162. Novel and non-invasive diagnostic tools, such as biomarkers, are needed for early CRC detection. In order to reduce the fatality rate in this disease, Biomarkers are used as indicators of a biological state; and they are measurable in biological media. They can be used to distinguish between a diseased state and a normal state, thus aiding diagnostics, response to specific therapies, and screening for an early diagnosis. A gene list of potential CRC biomarkers was generated by mining two gene databases, namely: Oncomine and Gene Expression Atlas. A total of 44 candidate genes were identified, based on their location on the cell surface, using the Database for Annotation, Visualisation and Integrated Discovery. These 44 genes were then subjected to an in-depth literature mining. The literature search parameters in PubMed, PubMed Central, Google Scholar and Science direct revealed publications showing that 23 genes were validated, while 21 genes were not validated. Nineteen genes were selected for gene validation in human colorectal cancer and healthy tissue of twelve patients. Total RNA was extracted from 12 colorectal cancer and 12 healthy tissue samples. The RNA was then quantified and reverse-transcribed into cDNA for gene expression analysis. The qPCR running conditions were optimized, by running a melting curve, in order to determine the optimum annealing temperatures. Primarily melt curves were run for nineteen of these twenty-one genes. Melt-curve analysis showed that nine genes were poor candidates for further validation studies; and therefore, only ten genes, namely: AGTRAP, ANKRD46, BACE2, CFB, CIAO1, NOMO3, PTDSS1, SLC5A6, TNFRSF12A and ZDHHC9 were validated by qPCR in human resected colorectal carcinoma and the normal tissues of twelve patients. The qPCR results showed that ZDHHC9 and the SLC5A6 genes were the only two statistically significant ones; and they were found to be down-regulated in human colorectal cancer vs healthy tissue samples.
- Full Text:
- Date Issued: 2014
Identifying appropriate paths for establishing sustainable irrigated crop based farming business on smallholder irrigation schemes: a case of Ncora Irrigation Scheme
- Authors: Mbizana, Nandipha
- Date: 2014
- Subjects: Crops -- Irrigation -- South Africa -- Eastern Cape , Crops -- Water requirements -- South Africa -- Eastern Cape , Agricultural extension work -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc Agric (Agricultural Economics)
- Identifier: vital:11213 , http://hdl.handle.net/10353/d1016205 , Crops -- Irrigation -- South Africa -- Eastern Cape , Crops -- Water requirements -- South Africa -- Eastern Cape , Agricultural extension work -- South Africa -- Eastern Cape
- Description: The study examined the impact of small scale irrigation technology in crop production under Ncora areas of Cofimvaba. To achieve the objective of the study, data were collected from 212 farmers engaged in various crop enterprises under the Ncora. The farmers were randomly selected. Descriptive Statistics, DEA model, linear regression model and gross margin analysis were used to analyse the results obtained from the survey. The descriptive results showed that Ncora farmers are small-scale farmers cultivating small hectare of land and using simple farm tools, mainly using furrow irrigation. Furthermore, they produce more than one crop enterprises. The gross margin Analysis shows that Ncora cultivation is profitable. The most profitable crop was found to be maize than potatoes. Farm production function revealed that land, labour and purchased inputs had a positive relationship with the output of the enterprises. SPSS was used to run data for linear regression model (OLS). It was suggested that extension services and private organizations assist farmers especially the emerging ones via provision of training, processing and storage facilities. Furthermore, continuous monitoring of soil and water quality as well as ground water table was recommended, in order to ensure sustainability of Ncora irrigation in the area.
- Full Text:
- Date Issued: 2014
- Authors: Mbizana, Nandipha
- Date: 2014
- Subjects: Crops -- Irrigation -- South Africa -- Eastern Cape , Crops -- Water requirements -- South Africa -- Eastern Cape , Agricultural extension work -- South Africa -- Eastern Cape
- Language: English
- Type: Thesis , Masters , MSc Agric (Agricultural Economics)
- Identifier: vital:11213 , http://hdl.handle.net/10353/d1016205 , Crops -- Irrigation -- South Africa -- Eastern Cape , Crops -- Water requirements -- South Africa -- Eastern Cape , Agricultural extension work -- South Africa -- Eastern Cape
- Description: The study examined the impact of small scale irrigation technology in crop production under Ncora areas of Cofimvaba. To achieve the objective of the study, data were collected from 212 farmers engaged in various crop enterprises under the Ncora. The farmers were randomly selected. Descriptive Statistics, DEA model, linear regression model and gross margin analysis were used to analyse the results obtained from the survey. The descriptive results showed that Ncora farmers are small-scale farmers cultivating small hectare of land and using simple farm tools, mainly using furrow irrigation. Furthermore, they produce more than one crop enterprises. The gross margin Analysis shows that Ncora cultivation is profitable. The most profitable crop was found to be maize than potatoes. Farm production function revealed that land, labour and purchased inputs had a positive relationship with the output of the enterprises. SPSS was used to run data for linear regression model (OLS). It was suggested that extension services and private organizations assist farmers especially the emerging ones via provision of training, processing and storage facilities. Furthermore, continuous monitoring of soil and water quality as well as ground water table was recommended, in order to ensure sustainability of Ncora irrigation in the area.
- Full Text:
- Date Issued: 2014
Identity, nationalism and successful governance: with reference to South Sudan
- Authors: Poggi, Giovanni Corrado
- Date: 2014
- Subjects: Identity politics -- South Sudan , Nationalism -- South Sudan
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:8333 , http://hdl.handle.net/10948/d1020579
- Description: The study seeks to delineate the African socio-political environment through an analytical contextualisation of repetitive authoritative systems, which perpetuate exclusion and the formation of politicised identity. Through a process of historical evaluation of African politics since the majority of states became independent, the study attempts to test a constructed triangular supposition that explains why identity disputation persists at almost every level of African governance. Bearing in mind the almost natural progression of African politics towards identity contestation, the second overriding objective seeks to evaluate the secession of South Sudan as a possible preventative model for identity politicised conflict. In this fashion, the study delves into the politics of a previously unified Sudan; and the events that led South Sudan to eventually seek secession. The primary objective of the study is to evaluate the assumption that territorial secession in Africa is able to quell identity contestation and promote an opportunity for equitable democratic governance. To meet the above objectives, a comprehensive outlay of African socio-politics and governance will be utilised to frame the analysis. Firstly, the study seeks to elaborate on the historiography of African colonial legacy as providing the foundations of identity exclusive politics on the continent. In this way, considerable investigative reverence must be given to the respective policies of colonial administration, namely indirect rule and direct rule. The effects of either of these policies contend a type of socio-political conditioning of African elites and civil society that still persists at time of writing. The concentration of this endeavour will be focused towards indirect colonial policy most famously implemented by British colonialism. The effects of the British policy of ethnic and ethno-religious categorisation is vitally important to a greater understanding of the majority of examples studied in the literature, including the formation of identity contestation in the case of the Sudan. Secondly, to further understand the complex dynamism of African politics which lead to identity based disputation, the study will turn to an analysis of the rhetoric of African independent governance and ideology. The primary objective here will be to detail how differing enactments of African ideology, including the advent of Pan-Arabism to the case of Sudan, ultimately continued the tradition of exclusive citizenship and dominance of some groups over others on the continent. This leads the study to uncover the deeper reasons for why socio-political exclusion continues to the present day. The final dimension of the triangular process suggests that exclusion has been maintained in Africa to prevent access of subverted groups to governance structures and more importantly to the limited resources of African states. Finally, the case of the secession of South Sudan is interpreted through the analytical lens of politicised identity that forms in the face of inadequate state structures to provide legitimate democratic access to the state. The third facet of the proposed theoretical triangle suggests that conflict and contestation is a product of grievances expressed from political exclusion. In this way, it is pivotal to the study to assess whether secession, as in the case of South Sudan, provides a valid alternative platform for suppressing identity contestation and promoting effective democratic consolidation. By all accounts, there is overwhelming evidence already to suggest that secession may be a successful way to repress identity politicisation. However, there remain substantial hurdles for many African states, including a now autonomous South Sudan, in order to finally dissolve the enduring problems of socio-political exclusion. Propositions and possible solutions will be posited for these states as an ad hoc objective.
- Full Text:
- Date Issued: 2014
- Authors: Poggi, Giovanni Corrado
- Date: 2014
- Subjects: Identity politics -- South Sudan , Nationalism -- South Sudan
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:8333 , http://hdl.handle.net/10948/d1020579
- Description: The study seeks to delineate the African socio-political environment through an analytical contextualisation of repetitive authoritative systems, which perpetuate exclusion and the formation of politicised identity. Through a process of historical evaluation of African politics since the majority of states became independent, the study attempts to test a constructed triangular supposition that explains why identity disputation persists at almost every level of African governance. Bearing in mind the almost natural progression of African politics towards identity contestation, the second overriding objective seeks to evaluate the secession of South Sudan as a possible preventative model for identity politicised conflict. In this fashion, the study delves into the politics of a previously unified Sudan; and the events that led South Sudan to eventually seek secession. The primary objective of the study is to evaluate the assumption that territorial secession in Africa is able to quell identity contestation and promote an opportunity for equitable democratic governance. To meet the above objectives, a comprehensive outlay of African socio-politics and governance will be utilised to frame the analysis. Firstly, the study seeks to elaborate on the historiography of African colonial legacy as providing the foundations of identity exclusive politics on the continent. In this way, considerable investigative reverence must be given to the respective policies of colonial administration, namely indirect rule and direct rule. The effects of either of these policies contend a type of socio-political conditioning of African elites and civil society that still persists at time of writing. The concentration of this endeavour will be focused towards indirect colonial policy most famously implemented by British colonialism. The effects of the British policy of ethnic and ethno-religious categorisation is vitally important to a greater understanding of the majority of examples studied in the literature, including the formation of identity contestation in the case of the Sudan. Secondly, to further understand the complex dynamism of African politics which lead to identity based disputation, the study will turn to an analysis of the rhetoric of African independent governance and ideology. The primary objective here will be to detail how differing enactments of African ideology, including the advent of Pan-Arabism to the case of Sudan, ultimately continued the tradition of exclusive citizenship and dominance of some groups over others on the continent. This leads the study to uncover the deeper reasons for why socio-political exclusion continues to the present day. The final dimension of the triangular process suggests that exclusion has been maintained in Africa to prevent access of subverted groups to governance structures and more importantly to the limited resources of African states. Finally, the case of the secession of South Sudan is interpreted through the analytical lens of politicised identity that forms in the face of inadequate state structures to provide legitimate democratic access to the state. The third facet of the proposed theoretical triangle suggests that conflict and contestation is a product of grievances expressed from political exclusion. In this way, it is pivotal to the study to assess whether secession, as in the case of South Sudan, provides a valid alternative platform for suppressing identity contestation and promoting effective democratic consolidation. By all accounts, there is overwhelming evidence already to suggest that secession may be a successful way to repress identity politicisation. However, there remain substantial hurdles for many African states, including a now autonomous South Sudan, in order to finally dissolve the enduring problems of socio-political exclusion. Propositions and possible solutions will be posited for these states as an ad hoc objective.
- Full Text:
- Date Issued: 2014
Imagine/nation : mediating 'xenophobia' through visual and performance art
- Machona, Gerald Ralph Tawanda
- Authors: Machona, Gerald Ralph Tawanda
- Date: 2014
- Subjects: Xenophobia -- South Africa , Xenophobia in mass media , Performance art , Immigrants in art , Violence in art , Race in art
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:2480 , http://hdl.handle.net/10962/d1011106 , Xenophobia -- South Africa , Xenophobia in mass media , Performance art , Immigrants in art , Violence in art , Race in art
- Description: This half-thesis has developed as a supporting document to an exhibition titled Vabvakure, people from far away, which responds to the growing trends of violence perpetrated against African foreign nationals living in South Africa. This violence which has generally been termed as 'xenophobia' has been framed within this discourse as 'afrophobia', as it is fraught with complexities of race, ethnicity and class. Evidently, not all foreign nationals are at risk but selective targeting of working class black African foreign nationals seems to be the modus operandi. Fanning these flames of prejudice are stereotypes and negative perceptions of Africa and African immigrants that have permeated into the national consciousness of South Africa, which the mainstream media has been complicit in cultivating. My practice is concerned with challenging this politic of representation in relation to the image of the African foreign national within South African society, who have been presented negatively and labelled as the 'Makwerekwere', the 'bogeymen' that have been blamed for the country’s current woes. In response to this, my research adopts the premise that forms of cultural mediation such as visual and performance art can offer further insights and possibly yield solutions that can be used to address these sentiments. As globalisation and neoliberal ideologies reshape the world, there is a growing need in the post-colonial state to revisit and re-construct notions of individual and collective identity, especially that of the nation. Nations, nationalisms and citizenry can no longer be defined solely through indigeneity, for as a result of radical shifts in the flow of migration and immigration policies that allow for naturalisation of aliens and foreign nationals, we are now faced with burgeoning levels of social diversity to the extent that constructions of nationhood that are based on the concept of autochthony have resulted in the persecution of the ‘other’.
- Full Text:
- Date Issued: 2014
- Authors: Machona, Gerald Ralph Tawanda
- Date: 2014
- Subjects: Xenophobia -- South Africa , Xenophobia in mass media , Performance art , Immigrants in art , Violence in art , Race in art
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:2480 , http://hdl.handle.net/10962/d1011106 , Xenophobia -- South Africa , Xenophobia in mass media , Performance art , Immigrants in art , Violence in art , Race in art
- Description: This half-thesis has developed as a supporting document to an exhibition titled Vabvakure, people from far away, which responds to the growing trends of violence perpetrated against African foreign nationals living in South Africa. This violence which has generally been termed as 'xenophobia' has been framed within this discourse as 'afrophobia', as it is fraught with complexities of race, ethnicity and class. Evidently, not all foreign nationals are at risk but selective targeting of working class black African foreign nationals seems to be the modus operandi. Fanning these flames of prejudice are stereotypes and negative perceptions of Africa and African immigrants that have permeated into the national consciousness of South Africa, which the mainstream media has been complicit in cultivating. My practice is concerned with challenging this politic of representation in relation to the image of the African foreign national within South African society, who have been presented negatively and labelled as the 'Makwerekwere', the 'bogeymen' that have been blamed for the country’s current woes. In response to this, my research adopts the premise that forms of cultural mediation such as visual and performance art can offer further insights and possibly yield solutions that can be used to address these sentiments. As globalisation and neoliberal ideologies reshape the world, there is a growing need in the post-colonial state to revisit and re-construct notions of individual and collective identity, especially that of the nation. Nations, nationalisms and citizenry can no longer be defined solely through indigeneity, for as a result of radical shifts in the flow of migration and immigration policies that allow for naturalisation of aliens and foreign nationals, we are now faced with burgeoning levels of social diversity to the extent that constructions of nationhood that are based on the concept of autochthony have resulted in the persecution of the ‘other’.
- Full Text:
- Date Issued: 2014
Imagining Civil Society in Zimbabwe and ‘Most of the World’:
- Authors: Helliker, Kirk D
- Date: 2014
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/144802 , vital:38380 , ISBN 9781461482628 , DOI: 10.1007/978-1-4614-8262-8_11
- Description: This chapter re-visits the notion of civil society in what Partha Chatterjee (The Politics of the Governed, 2004) calls ‘most of the world’ (beyond the capitalist metropoles) and, in doing so, uses Zimbabwe (and Africa more broadly) as an entry point into the literature on civil society. This chapter consists of four main sections. First, I discuss literature on civil society in Africa which, in the main, dichotomises civil society and the state empirically without any sustained theoretical reflections. Second, I provide an overview of Zimbabwean society and politics over the past decade and the ensuing debate, which in many ways produces a Manichean dualism whereby civil society is equated with progression and the state with regression. Third, I locate this conceptualisation of civil society within the broader international literature on civil society. These three sections, as a whole, highlight slippages in defining and understanding civil society: between civil society as a set of empirically identifiable organisational formations and civil society as a social space marked by civil liberties and voluntary arrangements in bourgeois society. Finally, I reimagine civil society in relation to ‘most of the world’.
- Full Text:
- Date Issued: 2014
- Authors: Helliker, Kirk D
- Date: 2014
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/144802 , vital:38380 , ISBN 9781461482628 , DOI: 10.1007/978-1-4614-8262-8_11
- Description: This chapter re-visits the notion of civil society in what Partha Chatterjee (The Politics of the Governed, 2004) calls ‘most of the world’ (beyond the capitalist metropoles) and, in doing so, uses Zimbabwe (and Africa more broadly) as an entry point into the literature on civil society. This chapter consists of four main sections. First, I discuss literature on civil society in Africa which, in the main, dichotomises civil society and the state empirically without any sustained theoretical reflections. Second, I provide an overview of Zimbabwean society and politics over the past decade and the ensuing debate, which in many ways produces a Manichean dualism whereby civil society is equated with progression and the state with regression. Third, I locate this conceptualisation of civil society within the broader international literature on civil society. These three sections, as a whole, highlight slippages in defining and understanding civil society: between civil society as a set of empirically identifiable organisational formations and civil society as a social space marked by civil liberties and voluntary arrangements in bourgeois society. Finally, I reimagine civil society in relation to ‘most of the world’.
- Full Text:
- Date Issued: 2014