An investigation of Wikipedia translation as an additive pedagogy for Oshikwanyama first language learning
- Authors: Hautemo, Aletta Mweneni
- Date: 2014
- Subjects: Kuanyama language -- Study and teaching (Secondary) -- Computer-assisted instruction , Kuanyama language -- Machine translating -- Namibia , Language and languages -- Study and teaching (Secondary) -- Computer-assisted instruction , Computer-assisted instruction -- Study and teaching (Secondary) -- Namibia , Information technology -- Study and teaching (Secondary) -- Namibia , Educational technology -- Namibia , Wikipedia
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1982 , http://hdl.handle.net/10962/d1013139
- Description: The integration of Information and Communication Technology in the indigenous language classroom lags behind compared to other subjects. In many ways, indigenous language teachers find it difficult and to some extent, impossible to integrate ICT into their classroom activities. The focus of this study is to explore the ways in which ICT could be used as a learning tool in an Oshikwanyama First Language classroom. I investigated the use of Wikipedia translation as an additional teaching and learning tool. I concentrated on the impact that ICT tools have on learning, and the motivation it has on learners to learn Oshikwanyama. This qualitative case study was conducted in an urban school in northern Namibia. The adoption of ICT at the school is good as there is a full-fledged computer lab with unlimited wireless internet access. This was a requirement for the project to enable the participants to work online. I purposefully chose higher-level learners (Secondary phase) for this study. I conducted a survey with them on their access to and use of ICT devices in their daily lives, and thereafter conducted a basic computer workshop and a Wikipedia translation project with them. My research findings show that although the use of ICT is part of the learners’ lives, most of the communication through ICT devices is done in English not Oshikwanyama. Wikipedia translation offers a stimulating learning platform for learners to learn Oshikwanyama and English at the same time and this improved their performance in both languages. Furthermore, the Wikipedia translation, which was done collaboratively, gave learners the confidence to work with other learners to create knowledge. Lastly, Wikipedia translation motivates learners to learn Oshikwanyama and use it in their daily ICT interaction.
- Full Text:
- Authors: Hautemo, Aletta Mweneni
- Date: 2014
- Subjects: Kuanyama language -- Study and teaching (Secondary) -- Computer-assisted instruction , Kuanyama language -- Machine translating -- Namibia , Language and languages -- Study and teaching (Secondary) -- Computer-assisted instruction , Computer-assisted instruction -- Study and teaching (Secondary) -- Namibia , Information technology -- Study and teaching (Secondary) -- Namibia , Educational technology -- Namibia , Wikipedia
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1982 , http://hdl.handle.net/10962/d1013139
- Description: The integration of Information and Communication Technology in the indigenous language classroom lags behind compared to other subjects. In many ways, indigenous language teachers find it difficult and to some extent, impossible to integrate ICT into their classroom activities. The focus of this study is to explore the ways in which ICT could be used as a learning tool in an Oshikwanyama First Language classroom. I investigated the use of Wikipedia translation as an additional teaching and learning tool. I concentrated on the impact that ICT tools have on learning, and the motivation it has on learners to learn Oshikwanyama. This qualitative case study was conducted in an urban school in northern Namibia. The adoption of ICT at the school is good as there is a full-fledged computer lab with unlimited wireless internet access. This was a requirement for the project to enable the participants to work online. I purposefully chose higher-level learners (Secondary phase) for this study. I conducted a survey with them on their access to and use of ICT devices in their daily lives, and thereafter conducted a basic computer workshop and a Wikipedia translation project with them. My research findings show that although the use of ICT is part of the learners’ lives, most of the communication through ICT devices is done in English not Oshikwanyama. Wikipedia translation offers a stimulating learning platform for learners to learn Oshikwanyama and English at the same time and this improved their performance in both languages. Furthermore, the Wikipedia translation, which was done collaboratively, gave learners the confidence to work with other learners to create knowledge. Lastly, Wikipedia translation motivates learners to learn Oshikwanyama and use it in their daily ICT interaction.
- Full Text:
Analysis of the interaction of Hsp90 with the extracellular matrix protein fibronectin (FN)
- Authors: Hunter, Morgan Campbell
- Date: 2014
- Subjects: Heat shock proteins , Fibronectins , Extracellular matrix proteins , Breast -- Cancer
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4170 , http://hdl.handle.net/10962/d1020960
- Description: Mounting evidence suggests that Hsp90 is present and functionally active in the extracellular space. The biological function of extracellular Hsp90 (eHsp90) remains relatively uncharacterized compared to that of intracellular Hsp90. eHsp90 has been shown to interact with a finite number of extracellular proteins, however, despite the identification of eHsp90 interacting proteins, the function of eHsp90 in these complexes is unknown. Several reports suggest a role for eHsp90α in cell migration and invasion. Reported targets for eHsp90 stimulated cell migration include MMPs, LRP-1, tyrosine kinase receptors and possible others unidentified. Limited studies report a role for eHsp90β. Recently, Hsp90α and Hsp90β were isolated in a complex containing fibronectin (FN) on the surface of MDA-MB-231 breast cancer cells. Herein, we report direct binding of Hsp90α and Hsp90β to FN using a solid phase binding assay and surface plasmon resonance (SPR) spectroscopy. SPR spectroscopy showed that Hsp90β bound the 70 kDa amino-terminal fragment of FN (FN70), but that binding of FN to Hsp90β was not limited to FN70. Confocal microscopy showed regions of colocalization of Hsp90 with extracellular FN matrix fibrils in Hs578T breast cancer cell lines. Treatment of Hs578T breast cancer cells with novobiocin (an Hsp90 inhibitor) and an LRP-1 blocking antibody resulted in a loss of FN matrix and FN endocytosis (novobiocin treated). Addition of exogenous Hsp90β was able to recover such effect after both treatments. FN was shown to colocalize with intracellular LRP-1 in novobiocin treated Hs578T cells. Immunoprecipitation of an LRP-1 containing complex showed the presence of Hsp90 and 70 and 120+ kDa FN fragments. Treatment of Hs578T cells with novobiocin increased the level of FN120+ bound in LRP-1 immunoprecipitate. Exogenous Hsp90β decreased the level of low and high molecular weight FN fragments in a complex with LRP-1, despite the fact that higher levels of lower molecular weight FN fragments were detected in this cell lysate compared to the other treatments. We report FN as a novel interacting protein of eHsp90. Taken together, we provide evidence for a direct role of eHsp90β in FN matrix remodeling. We suggest that Hsp90 plays a direct role in FN matrix dynamics through interaction with FN and LRP-1. The identification of FN as a novel interacting protein of eHsp90 suggests a role for Hsp90 in FN matrix remodeling, which is important for a number of fundamental cellular processes including cell migration and metastasis.
- Full Text:
- Authors: Hunter, Morgan Campbell
- Date: 2014
- Subjects: Heat shock proteins , Fibronectins , Extracellular matrix proteins , Breast -- Cancer
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4170 , http://hdl.handle.net/10962/d1020960
- Description: Mounting evidence suggests that Hsp90 is present and functionally active in the extracellular space. The biological function of extracellular Hsp90 (eHsp90) remains relatively uncharacterized compared to that of intracellular Hsp90. eHsp90 has been shown to interact with a finite number of extracellular proteins, however, despite the identification of eHsp90 interacting proteins, the function of eHsp90 in these complexes is unknown. Several reports suggest a role for eHsp90α in cell migration and invasion. Reported targets for eHsp90 stimulated cell migration include MMPs, LRP-1, tyrosine kinase receptors and possible others unidentified. Limited studies report a role for eHsp90β. Recently, Hsp90α and Hsp90β were isolated in a complex containing fibronectin (FN) on the surface of MDA-MB-231 breast cancer cells. Herein, we report direct binding of Hsp90α and Hsp90β to FN using a solid phase binding assay and surface plasmon resonance (SPR) spectroscopy. SPR spectroscopy showed that Hsp90β bound the 70 kDa amino-terminal fragment of FN (FN70), but that binding of FN to Hsp90β was not limited to FN70. Confocal microscopy showed regions of colocalization of Hsp90 with extracellular FN matrix fibrils in Hs578T breast cancer cell lines. Treatment of Hs578T breast cancer cells with novobiocin (an Hsp90 inhibitor) and an LRP-1 blocking antibody resulted in a loss of FN matrix and FN endocytosis (novobiocin treated). Addition of exogenous Hsp90β was able to recover such effect after both treatments. FN was shown to colocalize with intracellular LRP-1 in novobiocin treated Hs578T cells. Immunoprecipitation of an LRP-1 containing complex showed the presence of Hsp90 and 70 and 120+ kDa FN fragments. Treatment of Hs578T cells with novobiocin increased the level of FN120+ bound in LRP-1 immunoprecipitate. Exogenous Hsp90β decreased the level of low and high molecular weight FN fragments in a complex with LRP-1, despite the fact that higher levels of lower molecular weight FN fragments were detected in this cell lysate compared to the other treatments. We report FN as a novel interacting protein of eHsp90. Taken together, we provide evidence for a direct role of eHsp90β in FN matrix remodeling. We suggest that Hsp90 plays a direct role in FN matrix dynamics through interaction with FN and LRP-1. The identification of FN as a novel interacting protein of eHsp90 suggests a role for Hsp90 in FN matrix remodeling, which is important for a number of fundamental cellular processes including cell migration and metastasis.
- Full Text:
Analysis of transcription factor binding specificity using ChIP-seq data.
- Authors: Kibet, Caleb Kipkurui
- Date: 2014
- Subjects: Transcription factors , Chronic myeloid leukemia , Antioncogenes , Cancer cells -- Growth -- Regulation
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4115 , http://hdl.handle.net/10962/d1013131
- Description: Transcription factors (TFs) are key regulators of gene expression whose failure has been implicated in many diseases, including cancer. They bind at various sites at different specificity depending on the prevailing cellular conditions, disease, development stage or environmental conditions of the cell. TF binding specificity is how well a TF distinguishes functional sites from potential non-functional sites to form a useful regulatory network. Owing to its role in diseases, various techniques have been used to determine TF binding specificity in vitro and in vivo, including chromatin immuno-precipitation followed by massively parallel sequencing (ChIP-seq). ChIP-seq is an in vivo technique that considers how the chromatin landscape affects TF binding. Motif enrichment analysis (MEA) tools are used to identify motifs that are over-represented in ChIP-seq peak regions. One such tool, CentriMo, finds over-represented motifs at the center since peak calling software are biased to declaring binding regions centered at the TF binding site. In this study, we investigate the use of CentriMo and other MEA tools to determine the difference in motif enrichment attributed presence of Chronic Myeloid leukemia (CML)), treatment with Interferon (IFN) and Dexamethasone (DEX) compared to control based on Fisher’s exact test; using uniform peaks ChIP-seq data generated by the ENCODE consortium. CentriMo proved to be capable. We observed differential motif enrichment of TFs with tumor promoter activity: YY1, CEBPA, Egr1, Cmyc family, Gata1 and JunD in K562 while Stat1, Irf1, and Runx1 in Gm12878. Enrichment of CTCF in Gm12878 with YY1 as the immuno-precipitated (ChIP-ed) factor and the presence of significant spacing (SpaMo analysis) of CTCF and YY1 in Gm12878 but not in K562 could show that CTCF, as a repressor, helps in maintaining the required YY1 level in a normal cell line. IFN might reduce Cmyc and the Jun family of TFs binding via the repressive action of CTCF and E2f2. We also show that the concentration of DEX treatment affects motif enrichment with 50nm being an optimum concentration for Gr binding by maintaining open chromatin via AP1 TF. This study has demonstrated the usefulness of CentriMo for TF binding specificity analysis.
- Full Text:
- Authors: Kibet, Caleb Kipkurui
- Date: 2014
- Subjects: Transcription factors , Chronic myeloid leukemia , Antioncogenes , Cancer cells -- Growth -- Regulation
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4115 , http://hdl.handle.net/10962/d1013131
- Description: Transcription factors (TFs) are key regulators of gene expression whose failure has been implicated in many diseases, including cancer. They bind at various sites at different specificity depending on the prevailing cellular conditions, disease, development stage or environmental conditions of the cell. TF binding specificity is how well a TF distinguishes functional sites from potential non-functional sites to form a useful regulatory network. Owing to its role in diseases, various techniques have been used to determine TF binding specificity in vitro and in vivo, including chromatin immuno-precipitation followed by massively parallel sequencing (ChIP-seq). ChIP-seq is an in vivo technique that considers how the chromatin landscape affects TF binding. Motif enrichment analysis (MEA) tools are used to identify motifs that are over-represented in ChIP-seq peak regions. One such tool, CentriMo, finds over-represented motifs at the center since peak calling software are biased to declaring binding regions centered at the TF binding site. In this study, we investigate the use of CentriMo and other MEA tools to determine the difference in motif enrichment attributed presence of Chronic Myeloid leukemia (CML)), treatment with Interferon (IFN) and Dexamethasone (DEX) compared to control based on Fisher’s exact test; using uniform peaks ChIP-seq data generated by the ENCODE consortium. CentriMo proved to be capable. We observed differential motif enrichment of TFs with tumor promoter activity: YY1, CEBPA, Egr1, Cmyc family, Gata1 and JunD in K562 while Stat1, Irf1, and Runx1 in Gm12878. Enrichment of CTCF in Gm12878 with YY1 as the immuno-precipitated (ChIP-ed) factor and the presence of significant spacing (SpaMo analysis) of CTCF and YY1 in Gm12878 but not in K562 could show that CTCF, as a repressor, helps in maintaining the required YY1 level in a normal cell line. IFN might reduce Cmyc and the Jun family of TFs binding via the repressive action of CTCF and E2f2. We also show that the concentration of DEX treatment affects motif enrichment with 50nm being an optimum concentration for Gr binding by maintaining open chromatin via AP1 TF. This study has demonstrated the usefulness of CentriMo for TF binding specificity analysis.
- Full Text:
Antimicrobial properties and isotope investigations of South African honey
- Khan, F, Hill, Jaclyn M, Kaehler, Sven, Allsopp, M, van Vuuren, S
- Authors: Khan, F , Hill, Jaclyn M , Kaehler, Sven , Allsopp, M , van Vuuren, S
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/444539 , vital:74249 , https://doi.org/10.1111/jam.12533
- Description: The therapeutic potential of honey for the treatment of wound infections is well documented. However, South African (SA) honey has been poorly explored as an antimicrobial agent and given the well‐established antimicrobial properties of the indigenous plant species from SA, there is the potential that honey from this geographical region may exhibit noteworthy anti‐infective properties. In this study, the antimicrobial properties of 42 SA honey samples were determined.
- Full Text:
- Authors: Khan, F , Hill, Jaclyn M , Kaehler, Sven , Allsopp, M , van Vuuren, S
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/444539 , vital:74249 , https://doi.org/10.1111/jam.12533
- Description: The therapeutic potential of honey for the treatment of wound infections is well documented. However, South African (SA) honey has been poorly explored as an antimicrobial agent and given the well‐established antimicrobial properties of the indigenous plant species from SA, there is the potential that honey from this geographical region may exhibit noteworthy anti‐infective properties. In this study, the antimicrobial properties of 42 SA honey samples were determined.
- Full Text:
Application of carbon black and iron phthalocyanine composites in bioelectricity production at a brewery wastewater fed microbial fuel cell
- Mshoperi, Edith, Fogel, Ronen, Limson, Janice
- Authors: Mshoperi, Edith , Fogel, Ronen , Limson, Janice
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/431050 , vital:72740 , xlink:href="https://doi.org/10.1016/j.electacta.2013.11.01"
- Description: Aerobic cathode microbial fuel cells (MFCs) have been widely researched to provide bioremediation of wastewaters, coupled to sustainable energy production. In order to effectively accomplish this aim, suitable catalysts and catalyst supports for oxygen reduction reaction (ORR) are required. While iron phthaloycanine (FePc), supported on multi-walled carbon nanotubes has previously been studied for this function, cost of industrial production may hinder this. Importantly, this study examines the use of several available grades of carbon black for their relative suitability to perform as supports for FePc in MFC formats. Voltammetric studies showed that the different grades of carbon black provided varying lowering of the ORR overpotential, between ∼160 and ∼270 mV relative to unmodified GCEs, and an optimum grade (N326) was selected for further study. Carbon black/FePC composite electrodes exhibited comparable lowering of the ORR overpotential (606 mV) to potentials previously reported to nanotube/FePc composites (620 mV), as well as lowered charge-transfer resistance compared to electrodes solely modified with FePc. When applied as cathode modifiers in dual chambered MFCs utilising Enterobacter cloacae, the combined use of carbon black and FePc provided greater power densities than either alone; composite electrodes obtaining ∼400% power density, compared to unmodified electrodes. Modification of the anode with carbon black further increased power density, generating power densities an order of magnitude larger than those obtained at unmodified electrodes. The ability of beer brewery waste water (BBWW) to generate power at these modified surfaces yielded permissible power densities (∼40% that of reinforced clostridial media). Differences observed, in particular under agitation, are attributed to variations in nutrient content and nutrient complexity, between the two fuel substrates.
- Full Text:
- Authors: Mshoperi, Edith , Fogel, Ronen , Limson, Janice
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/431050 , vital:72740 , xlink:href="https://doi.org/10.1016/j.electacta.2013.11.01"
- Description: Aerobic cathode microbial fuel cells (MFCs) have been widely researched to provide bioremediation of wastewaters, coupled to sustainable energy production. In order to effectively accomplish this aim, suitable catalysts and catalyst supports for oxygen reduction reaction (ORR) are required. While iron phthaloycanine (FePc), supported on multi-walled carbon nanotubes has previously been studied for this function, cost of industrial production may hinder this. Importantly, this study examines the use of several available grades of carbon black for their relative suitability to perform as supports for FePc in MFC formats. Voltammetric studies showed that the different grades of carbon black provided varying lowering of the ORR overpotential, between ∼160 and ∼270 mV relative to unmodified GCEs, and an optimum grade (N326) was selected for further study. Carbon black/FePC composite electrodes exhibited comparable lowering of the ORR overpotential (606 mV) to potentials previously reported to nanotube/FePc composites (620 mV), as well as lowered charge-transfer resistance compared to electrodes solely modified with FePc. When applied as cathode modifiers in dual chambered MFCs utilising Enterobacter cloacae, the combined use of carbon black and FePc provided greater power densities than either alone; composite electrodes obtaining ∼400% power density, compared to unmodified electrodes. Modification of the anode with carbon black further increased power density, generating power densities an order of magnitude larger than those obtained at unmodified electrodes. The ability of beer brewery waste water (BBWW) to generate power at these modified surfaces yielded permissible power densities (∼40% that of reinforced clostridial media). Differences observed, in particular under agitation, are attributed to variations in nutrient content and nutrient complexity, between the two fuel substrates.
- Full Text:
Applications of lead phthalocyanines embedded in electrospun fibers for the photoinactivation of Escherichia coli in water
- Osifeko, Olawale L, Nyokong, Tebello
- Authors: Osifeko, Olawale L , Nyokong, Tebello
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/189898 , vital:44945 , xlink:href="https://doi.org/10.1016/j.dyepig.2014.05.010"
- Description: Lead (II) pyridyloxyphthalocyanine (PbTpyPc) and its quaternized form (PbTepyPc) were synthesized and the photophysical behavior examined. Low fluorescence quantum yields (ΦF) of 0.01 and 0.02 were observed for PbTepyPc and PbTpyPc, respectively. The singlet oxygen quantum yields (ΦΔ) were 0.60 and 0.68, for PbTepyPc and PbTpyPc, respectively using DPBF as a quencher in DMF. Singlet oxygen production of the embedded sensitizers in electrospun fiber were quantified using ADMA and were found to be ΦΔ = 0.41 and ΦΔ = 0.21 for PbTepyPc and PbTpyPc, respectively. Photodynamic inactivation of Escherichia coli (E. coli) with the quaternized photosensitizer at 5 μM, totally inactivated the E. coli (with log CFU = 10 decrease). Only 0.4 log CFU decrease was obtained with PbTpyPc. The embedded non-quaternized photosensitizer (PbTpyPc) was less active on the gram negative bacteria but the quaternized photosensitizer (PbTepyPc) was effective towards inactivation of E. coli.
- Full Text:
- Authors: Osifeko, Olawale L , Nyokong, Tebello
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/189898 , vital:44945 , xlink:href="https://doi.org/10.1016/j.dyepig.2014.05.010"
- Description: Lead (II) pyridyloxyphthalocyanine (PbTpyPc) and its quaternized form (PbTepyPc) were synthesized and the photophysical behavior examined. Low fluorescence quantum yields (ΦF) of 0.01 and 0.02 were observed for PbTepyPc and PbTpyPc, respectively. The singlet oxygen quantum yields (ΦΔ) were 0.60 and 0.68, for PbTepyPc and PbTpyPc, respectively using DPBF as a quencher in DMF. Singlet oxygen production of the embedded sensitizers in electrospun fiber were quantified using ADMA and were found to be ΦΔ = 0.41 and ΦΔ = 0.21 for PbTepyPc and PbTpyPc, respectively. Photodynamic inactivation of Escherichia coli (E. coli) with the quaternized photosensitizer at 5 μM, totally inactivated the E. coli (with log CFU = 10 decrease). Only 0.4 log CFU decrease was obtained with PbTpyPc. The embedded non-quaternized photosensitizer (PbTpyPc) was less active on the gram negative bacteria but the quaternized photosensitizer (PbTepyPc) was effective towards inactivation of E. coli.
- Full Text:
Applications of lead phthalocyanines embedded in electrospun fibers for the photoinactivation of Escherichia coli in water
- Osifeko, Olawale L, Nyokong, Tebello
- Authors: Osifeko, Olawale L , Nyokong, Tebello
- Date: 2014
- Language: English
- Type: Article
- Identifier: vital:7304 , http://hdl.handle.net/10962/d1020381
- Description: Lead (II) pyridyloxyphthalocyanine (PbTpyPc) and its quaternized form (PbTepyPc) were synthesized and the photophysical behavior examined. Low fluorescence quantum yields (ΦF) of 0.01 and 0.02 were observed for PbTepyPc and PbTpyPc, respectively. The singlet oxygen quantum yields (ΦΔ) were 0.60 and 0.68, for PbTepyPc and PbTpyPc, respectively using DPBF as a quencher in DMF. Singlet oxygen production of the embedded sensitizers in electrospun fiber were quantified using ADMA and were found to be ΦΔ = 0.41 and ΦΔ = 0.21 for PbTepyPc and PbTpyPc, respectively. Photodynamic inactivation of Escherichia coli (E. coli) with the quaternized photosensitizer at 5 μM, totally inactivated the E. coli (with log CFU = 10 decrease). Only 0.4 log CFU decrease was obtained with PbTpyPc. The embedded non-quaternized photosensitizer (PbTpyPc) was less active on the gram negative bacteria but the quaternized photosensitizer (PbTepyPc) was effective towards inactivation of E. coli. , Original publication is available at http://dx.doi.org/10.1016/j.dyepig.2014.05.010
- Full Text: false
- Authors: Osifeko, Olawale L , Nyokong, Tebello
- Date: 2014
- Language: English
- Type: Article
- Identifier: vital:7304 , http://hdl.handle.net/10962/d1020381
- Description: Lead (II) pyridyloxyphthalocyanine (PbTpyPc) and its quaternized form (PbTepyPc) were synthesized and the photophysical behavior examined. Low fluorescence quantum yields (ΦF) of 0.01 and 0.02 were observed for PbTepyPc and PbTpyPc, respectively. The singlet oxygen quantum yields (ΦΔ) were 0.60 and 0.68, for PbTepyPc and PbTpyPc, respectively using DPBF as a quencher in DMF. Singlet oxygen production of the embedded sensitizers in electrospun fiber were quantified using ADMA and were found to be ΦΔ = 0.41 and ΦΔ = 0.21 for PbTepyPc and PbTpyPc, respectively. Photodynamic inactivation of Escherichia coli (E. coli) with the quaternized photosensitizer at 5 μM, totally inactivated the E. coli (with log CFU = 10 decrease). Only 0.4 log CFU decrease was obtained with PbTpyPc. The embedded non-quaternized photosensitizer (PbTpyPc) was less active on the gram negative bacteria but the quaternized photosensitizer (PbTepyPc) was effective towards inactivation of E. coli. , Original publication is available at http://dx.doi.org/10.1016/j.dyepig.2014.05.010
- Full Text: false
Apprehension of bias and the spectacle of the fair-minded observer: a survey of recent commonwealth and South African decisions on pre-judgment
- Okpaluba, Chuks, Juma, Laurence
- Authors: Okpaluba, Chuks , Juma, Laurence
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/126107 , vital:35850 , http://specjuris.ufh.ac.za/apprehension-bias-and-spectacle-fair-minded-observer-survey-recent-commonwealth-and-south-african
- Description: A perusal of contemporary Commonwealth case law reveals that allegations of bias or apprehension of bias tend to revolve around the pecuniary or other interests of the Judge arising from kinship, previous relationship, or association with party or counsel.1Apparent bias could also be inferred from the judge‘s conduct or utterances, especially, his/her criticisms or cumulative criticisms of a party during proceedings or in adjudication.2In other instances, a judge‘s previous knowledge or association with the case in court, or of an important witness, may disqualify him/her from sitting or passing judgment in the case.3
- Full Text:
- Authors: Okpaluba, Chuks , Juma, Laurence
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/126107 , vital:35850 , http://specjuris.ufh.ac.za/apprehension-bias-and-spectacle-fair-minded-observer-survey-recent-commonwealth-and-south-african
- Description: A perusal of contemporary Commonwealth case law reveals that allegations of bias or apprehension of bias tend to revolve around the pecuniary or other interests of the Judge arising from kinship, previous relationship, or association with party or counsel.1Apparent bias could also be inferred from the judge‘s conduct or utterances, especially, his/her criticisms or cumulative criticisms of a party during proceedings or in adjudication.2In other instances, a judge‘s previous knowledge or association with the case in court, or of an important witness, may disqualify him/her from sitting or passing judgment in the case.3
- Full Text:
Argumentative and trustworthy scholars
- McKenna, Sioux, Boughey, Chrissie
- Authors: McKenna, Sioux , Boughey, Chrissie
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/187216 , vital:44580 , xlink:href="https://doi.org/10.1080/13562517.2014.934351"
- Description: Research-intensive universities, such as the Russell Group in the UK, the Ivy League Colleges in the USA and the Sandstone Universities in Australia, enjoy particular status in the higher education landscape. They are, however, also often associated with social elitism and selectivity, and this has led to critique as higher education systems seek to widen access. This article looks at how academic staff are discursively constructed in five such institutions in South Africa through an analysis of documentation submitted as part of a national review. Three interrelated discourses are identified: a discourse of ‘staff as scholars’ whereby research is privileged over teaching, a discourse of ‘academic argumentation’ whereby a critical disposition is valued and is called upon by academics to resist development initiatives and a discourse of ‘trust’ whereby it is assumed that academics share a value system and should thus be trusted to undertake quality teaching without interference.
- Full Text:
- Authors: McKenna, Sioux , Boughey, Chrissie
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/187216 , vital:44580 , xlink:href="https://doi.org/10.1080/13562517.2014.934351"
- Description: Research-intensive universities, such as the Russell Group in the UK, the Ivy League Colleges in the USA and the Sandstone Universities in Australia, enjoy particular status in the higher education landscape. They are, however, also often associated with social elitism and selectivity, and this has led to critique as higher education systems seek to widen access. This article looks at how academic staff are discursively constructed in five such institutions in South Africa through an analysis of documentation submitted as part of a national review. Three interrelated discourses are identified: a discourse of ‘staff as scholars’ whereby research is privileged over teaching, a discourse of ‘academic argumentation’ whereby a critical disposition is valued and is called upon by academics to resist development initiatives and a discourse of ‘trust’ whereby it is assumed that academics share a value system and should thus be trusted to undertake quality teaching without interference.
- Full Text:
Aspects of the ecology of leopards (Panthera Pardus) in the Little Karoo, South Africa
- Authors: Mann, Gareth
- Date: 2014
- Subjects: Leopard -- South Africa -- Little Karoo Leopard -- Monitoring -- South Africa -- Little Karoo Leopard -- Ecology -- South Africa -- Little Karoo Wildlife monitoring -- South Africa Wildlife conservation -- South Africa Animal populations -- South Africa -- Little Karoo Leopard -- Conservation -- South Africa -- Little Karoo
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5855 , http://hdl.handle.net/10962/d1012241
- Description: Leopards (Panthera pardus) are the most common large predators, free roaming outside of protected areas across most of South Africa. Leopard persistence is attributed to their tolerance of rugged terrain that is subject to less development pressure, as well as their cryptic behaviour. Nevertheless, existing leopard populations are threatened indirectly by ongoing transformation of natural habitat and directly through hunting and conflict with livestock farmers. Together these threats may further isolate leopards to fragmented areas of core natural habitat. I studied leopard habitat preferences, population density, diet and the attitudes of landowners towards leopards in the Little Karoo, Western Cape, South Africa, an area of mixed land-use that contains elements of three overlapping global biodiversity hotspots. Data were gathered between 2010 and 2012 using camera traps set up at 141 sites over an area of ~3100km², GPS tracking collars fitted to three male leopards, scat samples (n=76), interviews with landowners (n=53) analysed in combination with geographical information system (GIS) layers. My results reveal that leopards preferred rugged, mountainous terrain of intermediate elevation, avoiding low-lying, open areas where human disturbance was generally greater. Despite relatively un-fragmented habitat within my study area, the leopard population density (0.75 leopards/100km²) was one of the lowest yet recorded in South Africa. This may reflect low prey densities in mountain refuges in addition to historical human persecution in the area. Currently local landowners are more tolerant of leopards than other wildlife species with incidents of conflict involving leopards being rare relative to black-backed jackals (Canis mesomelas), baboons (Papio hamadryas), caracals (Caracal caracal) and porcupine (Hystrix africaeaustralis). Although current levels of conflict between leopards and stock farmers are low, leopards do depredate livestock, which constitute 10-15% of their diet. Improved livestock husbandry measures and co-operation between conservation authorities and farmers are necessary to mitigate such conflict and balance economic security with biodiversity conservation in the region. Leopards are the only remaining top predators throughout much of the Little Karoo and the Western Cape and as such are predicted to play a critical role in ecosystem structure and the survival of other species. Current high levels of connectivity between areas of suitable leopard habitat bode well for the conservation status of leopards within this region and future conservation efforts need to ensure that narrow corridors linking such habitat are preserved. The potential for leopards to serve as both an umbrella and a flagship species for biodiversity conservation suggests that long term monitoring of this population would be a conservation priority for the Little Karoo.
- Full Text:
- Authors: Mann, Gareth
- Date: 2014
- Subjects: Leopard -- South Africa -- Little Karoo Leopard -- Monitoring -- South Africa -- Little Karoo Leopard -- Ecology -- South Africa -- Little Karoo Wildlife monitoring -- South Africa Wildlife conservation -- South Africa Animal populations -- South Africa -- Little Karoo Leopard -- Conservation -- South Africa -- Little Karoo
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:5855 , http://hdl.handle.net/10962/d1012241
- Description: Leopards (Panthera pardus) are the most common large predators, free roaming outside of protected areas across most of South Africa. Leopard persistence is attributed to their tolerance of rugged terrain that is subject to less development pressure, as well as their cryptic behaviour. Nevertheless, existing leopard populations are threatened indirectly by ongoing transformation of natural habitat and directly through hunting and conflict with livestock farmers. Together these threats may further isolate leopards to fragmented areas of core natural habitat. I studied leopard habitat preferences, population density, diet and the attitudes of landowners towards leopards in the Little Karoo, Western Cape, South Africa, an area of mixed land-use that contains elements of three overlapping global biodiversity hotspots. Data were gathered between 2010 and 2012 using camera traps set up at 141 sites over an area of ~3100km², GPS tracking collars fitted to three male leopards, scat samples (n=76), interviews with landowners (n=53) analysed in combination with geographical information system (GIS) layers. My results reveal that leopards preferred rugged, mountainous terrain of intermediate elevation, avoiding low-lying, open areas where human disturbance was generally greater. Despite relatively un-fragmented habitat within my study area, the leopard population density (0.75 leopards/100km²) was one of the lowest yet recorded in South Africa. This may reflect low prey densities in mountain refuges in addition to historical human persecution in the area. Currently local landowners are more tolerant of leopards than other wildlife species with incidents of conflict involving leopards being rare relative to black-backed jackals (Canis mesomelas), baboons (Papio hamadryas), caracals (Caracal caracal) and porcupine (Hystrix africaeaustralis). Although current levels of conflict between leopards and stock farmers are low, leopards do depredate livestock, which constitute 10-15% of their diet. Improved livestock husbandry measures and co-operation between conservation authorities and farmers are necessary to mitigate such conflict and balance economic security with biodiversity conservation in the region. Leopards are the only remaining top predators throughout much of the Little Karoo and the Western Cape and as such are predicted to play a critical role in ecosystem structure and the survival of other species. Current high levels of connectivity between areas of suitable leopard habitat bode well for the conservation status of leopards within this region and future conservation efforts need to ensure that narrow corridors linking such habitat are preserved. The potential for leopards to serve as both an umbrella and a flagship species for biodiversity conservation suggests that long term monitoring of this population would be a conservation priority for the Little Karoo.
- Full Text:
Assessing the genetic diversity of catface rockcod epinephelus andersoni in the subtropical Western Indian Ocean and modelling the effects of climate change on their distribution
- Authors: Coppinger, Christine Rose
- Date: 2014
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/54499 , vital:26570
- Description: The catface rockcod Epinephelus andersoni is a range-restricted species that is endemic to the southeast coast of Africa from Quissico in Mozambique (subtropical) to Knysna in South Africa (warmtemperate). Its complex life-history, long-lived nature and high residency make E. andersoni potentially vulnerable to over-exploitation. Epinephelus andersoni is an important fishery species and has shown signs of depletion. Due to inadequate information necessary for management and conservation, further research is vital, particularly in the face of potentially significant climatic changes which could put further pressure on E. andersoni. The aim of the study was to provide information for the management of E. andersoni, with considerations for the possibly detrimental effects of future climate change. The objectives of this study were to describe the genetic structure and diversity of E. andersoni and to determine possible range shifts of E. andersoni with future changes in sea surface temperature. Genetic samples were collected throughout the distribution of E. andersoni. Standard DNA extraction and PCR using universal primers were conducted and nuclear (RPS7-1) and mitochondrial (cytochrome b) data were analysed to determine genetic diversity. A combination of nuclear and mitochondrial markers was used to ensure that the results were robust. RPS7-1 haplotype diversity was high (0.801) and an AMOVA on the RPS7-1 data showed significantly high among group variation (ΦCT = 0.204, p < 0.05) between five groups: 1. Quissico to Inhaca; 2. Cape Vidal to Port Edward; 3 Port St Johns to Coffee Bay; 4. Mbashe; 5. Port Alfred. This geographic structuring could be attributed to low gene flow across barriers such as the Port Alfred upwelling cell, the Mozambique Channel eddies and smaller more localised upwelling cells such as the Port St Johns cell. The cytochrome b results contrastingly indicate low haplotype diversity (0.309) and no differentiation (ΦCT = 0.265, p = 0.074) between groups and support the hypothesis of a historical population bottleneck. This may be due to an unusually slower mutation rate of the cytochrome b region than the RPS7-1 region, resulting in the RPS7-1 data showing a more recent picture of diversification. To complement the genetic results, niche modelling techniques were used to determine range shifts of E. andersoni with future temperature trends using species distribution and climatic data. The model illustrated a contraction of the E. andersoni distribution as well as future intensification of various upwelling cells along the south-east African coast including the Port Alfred upwelling cell. Due to the low gene flow across these barriers this intensification could decrease the resilience of E. andersoni, as its range becomes more limited with global change. The genetic data and modelling results combined provide useful information on which to base future fisheries management.
- Full Text:
- Authors: Coppinger, Christine Rose
- Date: 2014
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/54499 , vital:26570
- Description: The catface rockcod Epinephelus andersoni is a range-restricted species that is endemic to the southeast coast of Africa from Quissico in Mozambique (subtropical) to Knysna in South Africa (warmtemperate). Its complex life-history, long-lived nature and high residency make E. andersoni potentially vulnerable to over-exploitation. Epinephelus andersoni is an important fishery species and has shown signs of depletion. Due to inadequate information necessary for management and conservation, further research is vital, particularly in the face of potentially significant climatic changes which could put further pressure on E. andersoni. The aim of the study was to provide information for the management of E. andersoni, with considerations for the possibly detrimental effects of future climate change. The objectives of this study were to describe the genetic structure and diversity of E. andersoni and to determine possible range shifts of E. andersoni with future changes in sea surface temperature. Genetic samples were collected throughout the distribution of E. andersoni. Standard DNA extraction and PCR using universal primers were conducted and nuclear (RPS7-1) and mitochondrial (cytochrome b) data were analysed to determine genetic diversity. A combination of nuclear and mitochondrial markers was used to ensure that the results were robust. RPS7-1 haplotype diversity was high (0.801) and an AMOVA on the RPS7-1 data showed significantly high among group variation (ΦCT = 0.204, p < 0.05) between five groups: 1. Quissico to Inhaca; 2. Cape Vidal to Port Edward; 3 Port St Johns to Coffee Bay; 4. Mbashe; 5. Port Alfred. This geographic structuring could be attributed to low gene flow across barriers such as the Port Alfred upwelling cell, the Mozambique Channel eddies and smaller more localised upwelling cells such as the Port St Johns cell. The cytochrome b results contrastingly indicate low haplotype diversity (0.309) and no differentiation (ΦCT = 0.265, p = 0.074) between groups and support the hypothesis of a historical population bottleneck. This may be due to an unusually slower mutation rate of the cytochrome b region than the RPS7-1 region, resulting in the RPS7-1 data showing a more recent picture of diversification. To complement the genetic results, niche modelling techniques were used to determine range shifts of E. andersoni with future temperature trends using species distribution and climatic data. The model illustrated a contraction of the E. andersoni distribution as well as future intensification of various upwelling cells along the south-east African coast including the Port Alfred upwelling cell. Due to the low gene flow across these barriers this intensification could decrease the resilience of E. andersoni, as its range becomes more limited with global change. The genetic data and modelling results combined provide useful information on which to base future fisheries management.
- Full Text:
Assessment of the Lake Liambezi fishery, Zambezi region, Namibia
- Authors: Simasiku, Evans Kamwi
- Date: 2014
- Subjects: Fishery management -- Namibia -- Lake Liambezi , Seines -- Namibia -- Lake Liambezi , Gillnetting -- Namibia -- Lake Liambezi , Fishes -- Namibia -- Lake Liambezi , Fisheries -- Catch effort -- Namibia -- Lake Liambezi
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5366 , http://hdl.handle.net/10962/d1013036
- Description: Lake Liambezi in the Zambezi Region of Namibia, formerly known as the Caprivi Region, is shallow (<6m deep) and characterised by cyclic episodes of filling and drying. When full the lake supports a highly productive fishery and when dry the lake is completely dry and used for agriculture and grazing. In 2000 the lake filled, and between May 2011 and April 2012 was surveyed using beach seine, experimental gillnets and catch landing surveys to obtain information for conservation and management recommendations for the fishery. Littoral fishes in Lake Liambezi and the Kavango floodplain were sampled using seine net and physicochemical properties were measured. Seine net surveys demonstrated that Lake Liambezi littoral zones were dominated by fishes of the family Alestidae (59.7%) while Cichlids constituted the most diverse family. Juvenile Tilapia rendalli and Oreochromis macrochir were among the five most important species in the littoral zone, indicating that these commercially important species use the littoral zone as a nursery ground. Since T. rendalli and O. macrochir are commercially important species, because of this, it is advised that seine nets should not be used. Catch efficiency between monofilament and multifilament gillnets in Lake Liambezi offshore waters were assessed. Catch efficiency experimental fishing trials showed that monofilament gillnets catch per unit effort (CPUE) was three times higher than that of multifilament gillnets for Oreochromis andersonii, O. macrochir, T. rendalli, Serranochromis macrocephalus and Clarias spp. Oreochromis andersonii comprised over 66% of the overall CPUE for both net types combined indicating the importance of this species in the gillnet fishery on the lake. A fishery has been established on the lake with more than 300 canoes and 120 fishermen using monofilament and multifilament gillnets. CPUE was 15 kg/canoe/day and was significantly (P < 0.05) associated with monthly temperature and moon phase parameters indicating that the fishery of Lake Liambezi may be altered by climate and environmental factors. Annual catch from Lake Liambezi was estimated at 3193t with an estimated productivity of 106kg/ha, suggesting that Lake Liambezi makes a significant contribution to the fish supply in the Zambezi Region. Recommendations were made to manage the Lake Liambezi fishery by imposing restrictions on effort (number of fishing boats), gear type, mesh sizes and access. Proper fisheries management and monitoring should incorporate climatic and environmental factors such as temperature and moon phase to meet the challenges of global climatic changes as well as other environmental issues.
- Full Text:
- Authors: Simasiku, Evans Kamwi
- Date: 2014
- Subjects: Fishery management -- Namibia -- Lake Liambezi , Seines -- Namibia -- Lake Liambezi , Gillnetting -- Namibia -- Lake Liambezi , Fishes -- Namibia -- Lake Liambezi , Fisheries -- Catch effort -- Namibia -- Lake Liambezi
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:5366 , http://hdl.handle.net/10962/d1013036
- Description: Lake Liambezi in the Zambezi Region of Namibia, formerly known as the Caprivi Region, is shallow (<6m deep) and characterised by cyclic episodes of filling and drying. When full the lake supports a highly productive fishery and when dry the lake is completely dry and used for agriculture and grazing. In 2000 the lake filled, and between May 2011 and April 2012 was surveyed using beach seine, experimental gillnets and catch landing surveys to obtain information for conservation and management recommendations for the fishery. Littoral fishes in Lake Liambezi and the Kavango floodplain were sampled using seine net and physicochemical properties were measured. Seine net surveys demonstrated that Lake Liambezi littoral zones were dominated by fishes of the family Alestidae (59.7%) while Cichlids constituted the most diverse family. Juvenile Tilapia rendalli and Oreochromis macrochir were among the five most important species in the littoral zone, indicating that these commercially important species use the littoral zone as a nursery ground. Since T. rendalli and O. macrochir are commercially important species, because of this, it is advised that seine nets should not be used. Catch efficiency between monofilament and multifilament gillnets in Lake Liambezi offshore waters were assessed. Catch efficiency experimental fishing trials showed that monofilament gillnets catch per unit effort (CPUE) was three times higher than that of multifilament gillnets for Oreochromis andersonii, O. macrochir, T. rendalli, Serranochromis macrocephalus and Clarias spp. Oreochromis andersonii comprised over 66% of the overall CPUE for both net types combined indicating the importance of this species in the gillnet fishery on the lake. A fishery has been established on the lake with more than 300 canoes and 120 fishermen using monofilament and multifilament gillnets. CPUE was 15 kg/canoe/day and was significantly (P < 0.05) associated with monthly temperature and moon phase parameters indicating that the fishery of Lake Liambezi may be altered by climate and environmental factors. Annual catch from Lake Liambezi was estimated at 3193t with an estimated productivity of 106kg/ha, suggesting that Lake Liambezi makes a significant contribution to the fish supply in the Zambezi Region. Recommendations were made to manage the Lake Liambezi fishery by imposing restrictions on effort (number of fishing boats), gear type, mesh sizes and access. Proper fisheries management and monitoring should incorporate climatic and environmental factors such as temperature and moon phase to meet the challenges of global climatic changes as well as other environmental issues.
- Full Text:
Assessment of the long-term response to rehabilitation of two wetlands in KwaZulu-Natal, South Africa:
- Cowden, Craig, Kotze, Donovan C, Ellery, William F N, Sieben, E J J
- Authors: Cowden, Craig , Kotze, Donovan C , Ellery, William F N , Sieben, E J J
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/144431 , vital:38345 , DOI: 10.2989/16085914.2014.954518
- Description: Assessing the ecological outcomes of wetland rehabilitation activities is an important need recognised by the ‘Working for Wetlands’ programme in South Africa. An assessment of ecological response was conducted in the Killarney and Kruisfontein wetlands, KwaZulu-Natal, in 2005 prior to rehabilitation in 2006, and again in 2011 and 2012, respectively, following rehabilitation. The assessment criteria included an evaluation of changes in ecological integrity, the supply of ecosystem services, and vegetation composition. Improvements in hydrological and geomorphic integrity were recorded in both wetlands, resulting in improved ecosystem delivery.
- Full Text:
- Authors: Cowden, Craig , Kotze, Donovan C , Ellery, William F N , Sieben, E J J
- Date: 2014
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/144431 , vital:38345 , DOI: 10.2989/16085914.2014.954518
- Description: Assessing the ecological outcomes of wetland rehabilitation activities is an important need recognised by the ‘Working for Wetlands’ programme in South Africa. An assessment of ecological response was conducted in the Killarney and Kruisfontein wetlands, KwaZulu-Natal, in 2005 prior to rehabilitation in 2006, and again in 2011 and 2012, respectively, following rehabilitation. The assessment criteria included an evaluation of changes in ecological integrity, the supply of ecosystem services, and vegetation composition. Improvements in hydrological and geomorphic integrity were recorded in both wetlands, resulting in improved ecosystem delivery.
- Full Text:
Audience observations of art, identity and schizophrenia : possibilities for identity movement
- Authors: Farquharson, Kirsten Leigh
- Date: 2014
- Subjects: Mental illness -- Social aspects , Stigma (Social psychology) , Identity (Psychology) , Schizophrenia -- Treatment , Art therapy -- Research , Art, South African -- 21st century -- Exhibitions
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3223 , http://hdl.handle.net/10962/d1012992
- Description: This research situates itself in the study of stigma in mental illness. In particular, the aim is to explore the potential that art making and exhibiting has in reducing stigma for those with a diagnosis of schizophrenia. The research explores one aspect (the exhibition stage) of an "art as therapy" project. The exhibiting of one’s artwork aims to counter limiting "patient" identities by allowing those labelled as psychiatric patients to extend their self-identity to an alternative identity of the "artist". However, this idea only stands strong if the artwork created is not discriminated against as "naïve art" and is accepted or at least considered for acceptance as legitimate nonprofessional artwork. This research explores the ways in which art created by inpatients with a diagnosis of schizophrenia is received by the general art-viewing public at the National Arts Festival in Grahamstown, South Africa. The study uses a discourse analytic framework to analyse the interviews of members of the public who attended the art exhibition of patient artwork. It will examine the ways in which the public construct the artworks and how they position the makers of this art across a continuum, from patient to artist. The results of this thesis have implications for rehabilitation practices for people with a diagnosis of schizophrenia particularly with regard to opportunities to "perform" alternative identities in public spaces.
- Full Text:
- Authors: Farquharson, Kirsten Leigh
- Date: 2014
- Subjects: Mental illness -- Social aspects , Stigma (Social psychology) , Identity (Psychology) , Schizophrenia -- Treatment , Art therapy -- Research , Art, South African -- 21st century -- Exhibitions
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:3223 , http://hdl.handle.net/10962/d1012992
- Description: This research situates itself in the study of stigma in mental illness. In particular, the aim is to explore the potential that art making and exhibiting has in reducing stigma for those with a diagnosis of schizophrenia. The research explores one aspect (the exhibition stage) of an "art as therapy" project. The exhibiting of one’s artwork aims to counter limiting "patient" identities by allowing those labelled as psychiatric patients to extend their self-identity to an alternative identity of the "artist". However, this idea only stands strong if the artwork created is not discriminated against as "naïve art" and is accepted or at least considered for acceptance as legitimate nonprofessional artwork. This research explores the ways in which art created by inpatients with a diagnosis of schizophrenia is received by the general art-viewing public at the National Arts Festival in Grahamstown, South Africa. The study uses a discourse analytic framework to analyse the interviews of members of the public who attended the art exhibition of patient artwork. It will examine the ways in which the public construct the artworks and how they position the makers of this art across a continuum, from patient to artist. The results of this thesis have implications for rehabilitation practices for people with a diagnosis of schizophrenia particularly with regard to opportunities to "perform" alternative identities in public spaces.
- Full Text:
Bacterial degradation of waste coal
- Authors: Madikiza, Lwazikazi
- Date: 2014
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/54576 , vital:26590
- Description: As an energy source coal has one of the largest agglomerations in the world. Consequently mining of coal creates large volumes of waste in the form of low ranks coals. The complex structure of coal makes it difficult for the microorganisms to degrade and relatively few bacteria and fungi have been shown to break down coal. This study aimed to investigate bacteria not previously known to degrade coal. In this study bacteria were isolated from hydrocarbon contaminated sites and inoculated in coal medium where coal served as the only carbon source. Three strains produced a yellow – brown supernatant after 14 d of incubation at 30 °C. Bacteria generating a yellow – brown coloured supernatant were presumed to possess coal degrading capabilities and the best performing of these bacterial species was identified using 16s rDNA as Bacillus flexus. Scanning electron microscopy showed that there was a close association between the bacterium and substrate coal. The close association of bacteria to substrate suggested that these organisms were able to maximize solubilisation. FT-IR spectroscopic analysis demonstrated the addition of single bonded compounds COOH, OH, CN and CH that were absent prior to bacterial interaction. The increase in oxygen rich regions indicated degradation of the coal substrate. Elemental analysis showed that there was a decrease in carbon content from 47 % to 24 % during the 14 day incubation period. Reduction in coal carbon content was assumed to be due to bacterial utilization for metabolism and growth particularly as untreated coal substrate showed minimal loss of carbon. Analysis of the residual culture medium revealed that there was a linear increase in humic-like substance concentration for 8 d, coincident with increased coal biosolubilisation and colour change. Laccase activity was insignificant, and at 13 d enzyme activity was only 5×10-3 U/L suggesting that B. flexus may use a different mechanism to degrade coal. Residual culture medium remaining after bacterial action on the coal substrate appeared to possess plant growth promoting activity. This soluble biodegradation product with characteristics similar to humic acid-like substances was shown to impact growth of radish cotyledons. Expansion of isolated radish cotyledons was enhanced by 140% when incubated in coal biodegradation product. In conclusion, this study has yielded B. flexus and two other unidentified bacteria, isolated from polyaromatic hydrocarbon contaminated soils, and demonstrated the ability of these microorganisms to degrade waste coal. Further studies to elucidate the mechanism of coal breakdown by B. flexus, synergies with other coal degrading microorganisms, and incorporation of bacterium into Fungcoal bioprocess technology is imminent.
- Full Text:
- Authors: Madikiza, Lwazikazi
- Date: 2014
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/54576 , vital:26590
- Description: As an energy source coal has one of the largest agglomerations in the world. Consequently mining of coal creates large volumes of waste in the form of low ranks coals. The complex structure of coal makes it difficult for the microorganisms to degrade and relatively few bacteria and fungi have been shown to break down coal. This study aimed to investigate bacteria not previously known to degrade coal. In this study bacteria were isolated from hydrocarbon contaminated sites and inoculated in coal medium where coal served as the only carbon source. Three strains produced a yellow – brown supernatant after 14 d of incubation at 30 °C. Bacteria generating a yellow – brown coloured supernatant were presumed to possess coal degrading capabilities and the best performing of these bacterial species was identified using 16s rDNA as Bacillus flexus. Scanning electron microscopy showed that there was a close association between the bacterium and substrate coal. The close association of bacteria to substrate suggested that these organisms were able to maximize solubilisation. FT-IR spectroscopic analysis demonstrated the addition of single bonded compounds COOH, OH, CN and CH that were absent prior to bacterial interaction. The increase in oxygen rich regions indicated degradation of the coal substrate. Elemental analysis showed that there was a decrease in carbon content from 47 % to 24 % during the 14 day incubation period. Reduction in coal carbon content was assumed to be due to bacterial utilization for metabolism and growth particularly as untreated coal substrate showed minimal loss of carbon. Analysis of the residual culture medium revealed that there was a linear increase in humic-like substance concentration for 8 d, coincident with increased coal biosolubilisation and colour change. Laccase activity was insignificant, and at 13 d enzyme activity was only 5×10-3 U/L suggesting that B. flexus may use a different mechanism to degrade coal. Residual culture medium remaining after bacterial action on the coal substrate appeared to possess plant growth promoting activity. This soluble biodegradation product with characteristics similar to humic acid-like substances was shown to impact growth of radish cotyledons. Expansion of isolated radish cotyledons was enhanced by 140% when incubated in coal biodegradation product. In conclusion, this study has yielded B. flexus and two other unidentified bacteria, isolated from polyaromatic hydrocarbon contaminated soils, and demonstrated the ability of these microorganisms to degrade waste coal. Further studies to elucidate the mechanism of coal breakdown by B. flexus, synergies with other coal degrading microorganisms, and incorporation of bacterium into Fungcoal bioprocess technology is imminent.
- Full Text:
Becoming and being: a critical realist study into the emergence of identity in emergency medical science students, and the construct of graduate attributes
- Authors: Millar, Bernadette Theresa
- Date: 2014
- Subjects: Critical realism , Emergency medical personnel -- Psychology , Emergency medical services
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1327 , http://hdl.handle.net/10962/d1013175
- Description: This critical realist thesis seeks to understand how student, graduate and professional identities emerge in Emergency Medical Science (EMS) students at a South African University of Technology (UoT) as well as in professional paramedics in the Emergency Medical Care Services (EMCS). It further considers the construct of graduate attributes (GAs) and its relationship to emergence of identity and influence on curriculum design. The research design is that of a case study. The theoretical framework is critical realism whose depth ontology posits three domains of reality. Causal powers and generative mechanisms exist in the Real domain which cause events or phenomena to emerge in the Actual domain that are experienced in the Empirical domain. Using retroduction one may come to explore some of the causes for the event. Using Bhaskar’s concepts of identity, the self, absence and emergence, ontology and four-planar social being, a Bhaskarian explanatory framework of identity to explore the emergence of identity has been created. In exploring graduate attributes, a critical realist question is posed: “What must the world be like for GAs to exist” to explore the possibilities of the existence of GAs. It was found that student identity emerges diachronically in three moments, while professional paramedic identity starts to emerge during the third year of study mainly through the structure, culture and agency of workplace-based learning. In answer to the critical realist question it was found that GAs emerge from the neoliberalist commodification of universities. In seeking an alternative to GAs, traits and attitudes were explored. It was found that these emerge from curriculum, interplay of departmental structure, culture and agency of and from students’ being which makes them ontologically radically different from GAs. This study concludes that student, graduate and professional identities emerge from a person’s core constellational identity diachronically within four-planar social being and the interplay of structure, culture and agency. GAs cannot be related to the emergence of identity and curriculum design because of their ontology; however, if traits and attitudes are substituted for GAs, a close relationship does exist between emergence of identity, traits and attitudes and curriculum design.
- Full Text:
- Authors: Millar, Bernadette Theresa
- Date: 2014
- Subjects: Critical realism , Emergency medical personnel -- Psychology , Emergency medical services
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:1327 , http://hdl.handle.net/10962/d1013175
- Description: This critical realist thesis seeks to understand how student, graduate and professional identities emerge in Emergency Medical Science (EMS) students at a South African University of Technology (UoT) as well as in professional paramedics in the Emergency Medical Care Services (EMCS). It further considers the construct of graduate attributes (GAs) and its relationship to emergence of identity and influence on curriculum design. The research design is that of a case study. The theoretical framework is critical realism whose depth ontology posits three domains of reality. Causal powers and generative mechanisms exist in the Real domain which cause events or phenomena to emerge in the Actual domain that are experienced in the Empirical domain. Using retroduction one may come to explore some of the causes for the event. Using Bhaskar’s concepts of identity, the self, absence and emergence, ontology and four-planar social being, a Bhaskarian explanatory framework of identity to explore the emergence of identity has been created. In exploring graduate attributes, a critical realist question is posed: “What must the world be like for GAs to exist” to explore the possibilities of the existence of GAs. It was found that student identity emerges diachronically in three moments, while professional paramedic identity starts to emerge during the third year of study mainly through the structure, culture and agency of workplace-based learning. In answer to the critical realist question it was found that GAs emerge from the neoliberalist commodification of universities. In seeking an alternative to GAs, traits and attitudes were explored. It was found that these emerge from curriculum, interplay of departmental structure, culture and agency of and from students’ being which makes them ontologically radically different from GAs. This study concludes that student, graduate and professional identities emerge from a person’s core constellational identity diachronically within four-planar social being and the interplay of structure, culture and agency. GAs cannot be related to the emergence of identity and curriculum design because of their ontology; however, if traits and attitudes are substituted for GAs, a close relationship does exist between emergence of identity, traits and attitudes and curriculum design.
- Full Text:
Behavior of palladium nanoparticles in the absence or presence of cobalt tetraaminophthalocyanine for the electrooxidation of hydrazine
- Maringa, Audacity, Nyokong, Tebello
- Authors: Maringa, Audacity , Nyokong, Tebello
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/189796 , vital:44932 , xlink:href="https://doi.org/10.1002/elan.201400028"
- Description: We report on the electrodeposition of palladium nanoparticles (PdNPs) on a glassy carbon electrode (GCE) and onto a poly-CoTAPc-GCE (CoTAPc=cobalt tetraamino phthalocyanine) surface. The electrodes are denoted as PdNPs-GCE and PdNPs/poly-CoTAPc-GCE, respectively. PdNPs/poly-CoTAPc-GCE showed the best activity for the oxidation of hydrazine at the lowest potential of −0.28 V and with the highest currents. The results were further supported by electrochemical impedance spectroscopy (EIS) which showed that there was less resistance to charge transfer for PdNPs/poly-CoTAPc-GCE compared to PdNPs-GCE. The catalytic rate constant for hydrazine oxidation was 6.12×108 cm3 mol−1 s−1 using PdNPs/poly-CoTAPc-GCE.
- Full Text:
- Authors: Maringa, Audacity , Nyokong, Tebello
- Date: 2014
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/189796 , vital:44932 , xlink:href="https://doi.org/10.1002/elan.201400028"
- Description: We report on the electrodeposition of palladium nanoparticles (PdNPs) on a glassy carbon electrode (GCE) and onto a poly-CoTAPc-GCE (CoTAPc=cobalt tetraamino phthalocyanine) surface. The electrodes are denoted as PdNPs-GCE and PdNPs/poly-CoTAPc-GCE, respectively. PdNPs/poly-CoTAPc-GCE showed the best activity for the oxidation of hydrazine at the lowest potential of −0.28 V and with the highest currents. The results were further supported by electrochemical impedance spectroscopy (EIS) which showed that there was less resistance to charge transfer for PdNPs/poly-CoTAPc-GCE compared to PdNPs-GCE. The catalytic rate constant for hydrazine oxidation was 6.12×108 cm3 mol−1 s−1 using PdNPs/poly-CoTAPc-GCE.
- Full Text:
Behavior of Palladium Nanoparticles in the Absence or Presence of Cobalt Tetraaminophthalocyanine for the Electrooxidation of Hydrazine
- Maringa, Audacity, Nyokong, Tebello
- Authors: Maringa, Audacity , Nyokong, Tebello
- Date: 2014
- Language: English
- Type: Article
- Identifier: vital:7305 , http://hdl.handle.net/10962/d1020382
- Description: We report on the electrodeposition of palladium nanoparticles (PdNPs) on a glassy carbon electrode (GCE) and onto a poly-CoTAPc-GCE (CoTAPc=cobalt tetraamino phthalocyanine) surface. The electrodes are denoted as PdNPs-GCE and PdNPs/poly-CoTAPc-GCE, respectively. PdNPs/poly-CoTAPc-GCE showed the best activity for the oxidation of hydrazine at the lowest potential of −0.28 V and with the highest currents. The results were further supported by electrochemical impedance spectroscopy (EIS) which showed that there was less resistance to charge transfer for PdNPs/poly-CoTAPc-GCE compared to PdNPs-GCE. The catalytic rate constant for hydrazine oxidation was 6.12×108 cm3 mol−1 s−1 using PdNPs/poly-CoTAPc-GCE. , Original publication is available at http://dx.doi.org/10.1002/elan.201400028
- Full Text: false
- Authors: Maringa, Audacity , Nyokong, Tebello
- Date: 2014
- Language: English
- Type: Article
- Identifier: vital:7305 , http://hdl.handle.net/10962/d1020382
- Description: We report on the electrodeposition of palladium nanoparticles (PdNPs) on a glassy carbon electrode (GCE) and onto a poly-CoTAPc-GCE (CoTAPc=cobalt tetraamino phthalocyanine) surface. The electrodes are denoted as PdNPs-GCE and PdNPs/poly-CoTAPc-GCE, respectively. PdNPs/poly-CoTAPc-GCE showed the best activity for the oxidation of hydrazine at the lowest potential of −0.28 V and with the highest currents. The results were further supported by electrochemical impedance spectroscopy (EIS) which showed that there was less resistance to charge transfer for PdNPs/poly-CoTAPc-GCE compared to PdNPs-GCE. The catalytic rate constant for hydrazine oxidation was 6.12×108 cm3 mol−1 s−1 using PdNPs/poly-CoTAPc-GCE. , Original publication is available at http://dx.doi.org/10.1002/elan.201400028
- Full Text: false
Bells as memorials in South Africa to the Great (1914-18) War
- Lewis, Colin A, De Wet, Tertius, Teugels, Jet L, Van Deventer, Pieta J U
- Authors: Lewis, Colin A , De Wet, Tertius , Teugels, Jet L , Van Deventer, Pieta J U
- Date: 2014
- Language: English
- Type: Article , text
- Identifier: vital:6192 , http://hdl.handle.net/10962/d1013418
- Description: The contribution of South Africa to the allied cause in the Great War, and the sacrifice of so many lives of the White and Coloured populations, is memorialised on bells of the Cape Town carillon, on ringing, and on clock and other bells. The contribution of the Black population awaits recognition. Restoration of the Cape Town carillon so that it can again be played effectively, would be a fitting memorial to those who lost their lives in the non-combative roles that were open to the majority population of South Africa. Completion of the ring at what is now Queenstown cathedral would also be a fitting tribute to the bravery and unstinting service of so many South Africans during the Great War. , Colin Lewis was Professor of Geography at Rhodes University, Grahamstown, South Africa from 1989 until his retirement at the end of 2007. In 1990, with the strong support of the incumbent Vice-Chancellor, Dr Derek Henderson, he instigated the Certificate in Change Ringing (Church Bell Ringing) in the Rhodes University Department of Music and Musicology - the first such course to be offered in Africa. Since that date he has lectured in the basic theory, and taught the practice of change ringing. He is the Ringing Master of the Cathedral of St Michael and St George, Grahamstown, South Africa.
- Full Text:
- Authors: Lewis, Colin A , De Wet, Tertius , Teugels, Jet L , Van Deventer, Pieta J U
- Date: 2014
- Language: English
- Type: Article , text
- Identifier: vital:6192 , http://hdl.handle.net/10962/d1013418
- Description: The contribution of South Africa to the allied cause in the Great War, and the sacrifice of so many lives of the White and Coloured populations, is memorialised on bells of the Cape Town carillon, on ringing, and on clock and other bells. The contribution of the Black population awaits recognition. Restoration of the Cape Town carillon so that it can again be played effectively, would be a fitting memorial to those who lost their lives in the non-combative roles that were open to the majority population of South Africa. Completion of the ring at what is now Queenstown cathedral would also be a fitting tribute to the bravery and unstinting service of so many South Africans during the Great War. , Colin Lewis was Professor of Geography at Rhodes University, Grahamstown, South Africa from 1989 until his retirement at the end of 2007. In 1990, with the strong support of the incumbent Vice-Chancellor, Dr Derek Henderson, he instigated the Certificate in Change Ringing (Church Bell Ringing) in the Rhodes University Department of Music and Musicology - the first such course to be offered in Africa. Since that date he has lectured in the basic theory, and taught the practice of change ringing. He is the Ringing Master of the Cathedral of St Michael and St George, Grahamstown, South Africa.
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Beyond black and white: black solidarity in post-apartheid South Africa
- Authors: Majavu, Phumlani
- Date: 2014
- Subjects: Black nationalism -- South Africa , Race relations -- South Africa , South Africa -- Politics and government -- 21st century
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2887 , http://hdl.handle.net/10962/d1016359
- Description: Almost 20 years after the white Nationalist government was voted out, some black South Africans believe that black solidarity is still necessary in South Africa. These people argue that since post-apartheid South Africa is still marred with racial injustice, it makes sense for blacks to advocate for black solidarity. Although it is true that black solidarity played an important role in the struggle against apartheid, in this thesis I argue that the struggle against current forms of racial injustice does not necessarily require black solidarity. This is not to deny the prevailing racialized oppression in the post-apartheid era, nor to deny the importance of black solidarity in the past; rather the point I am making is that the current form of racial oppression is somewhat different from the one before 1994. Hence I argue in this thesis that the current form of racial oppression requires us to do certain things differently. Doing things differently means improving upon the strategies of the past. For this to happen, I argue that every human being who believes in and is committed to racial justice ought to be included in the struggle for justice. Change, after all, is brought about when committed human beings work together for liberation and justice.
- Full Text:
- Authors: Majavu, Phumlani
- Date: 2014
- Subjects: Black nationalism -- South Africa , Race relations -- South Africa , South Africa -- Politics and government -- 21st century
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2887 , http://hdl.handle.net/10962/d1016359
- Description: Almost 20 years after the white Nationalist government was voted out, some black South Africans believe that black solidarity is still necessary in South Africa. These people argue that since post-apartheid South Africa is still marred with racial injustice, it makes sense for blacks to advocate for black solidarity. Although it is true that black solidarity played an important role in the struggle against apartheid, in this thesis I argue that the struggle against current forms of racial injustice does not necessarily require black solidarity. This is not to deny the prevailing racialized oppression in the post-apartheid era, nor to deny the importance of black solidarity in the past; rather the point I am making is that the current form of racial oppression is somewhat different from the one before 1994. Hence I argue in this thesis that the current form of racial oppression requires us to do certain things differently. Doing things differently means improving upon the strategies of the past. For this to happen, I argue that every human being who believes in and is committed to racial justice ought to be included in the struggle for justice. Change, after all, is brought about when committed human beings work together for liberation and justice.
- Full Text: