Formulation, development and assessment of tenofovir disoproxil fumarate-loaded pellets
- Authors: Dube, Tawanda
- Date: 2015
- Language: English
- Type: text , Thesis , Masters , MPharm
- Identifier: http://hdl.handle.net/10962/54690 , vital:26600
- Description: Tenofovir disoproxil fumarate (TDF) is a novel nucleotide analog reverse transcriptase inhibitor that is recommended by the WHO for use in first line treatment of HIV infections. Due to the high dose of TDF for anti-retroviral treatment the formulation of a pellet dosage form may improve patient adherence by incorporation of a large dose in a relatively small dosage form. TDF is currently only available in tablet form. A simple, sensitive, selective, rapid, accurate, precise, stability indicating reversed-phase HPLC method was developed and validated in accordance with ICH guidelines and was successfully used for the analysis of TDF raw material and pharmaceutical dosage forms. Preformulation studies included an investigation of TDF-excipient and excipient-excipient interactions with all materials that could potentially be used to produce extruded and spheronized pellets. Nuclear Magnetic Resonance spectroscopy (NMR), Infrared Spectroscopy (IR), Differential Scanning Colorimetry (DSC) and Thermogravimetric analysis were used for identification and purity testing of TDF and all excipients. DSC data revealed that no potential interactions between TDF and the excipients occurred suggesting that incompatibility reactions were unlikely during manufacture and storage. These findings were confirmed by IR analysis that revealed that no physical interaction was likely between any of the excipients used and TDF. DSC data also reveal the existence of the α and β-polymorphs of TDF as evidenced by two enthalpy changes observed on the resultant thermograms. The existence of two polymorphs is unlikely to result in incompatibility and was confirmed by IR analysis. The IR spectra reveal that all characteristic peaks for TDF were present in 1:1 binary mixtures. Therefore TDF is compatible with all excipients tested and thermal analysis confirmed the stability of TDF under manufacturing conditions. The temperature of degradation temperature established through DSC analysis confirmed that degradation during manufacture is unlikely as the temperature of manufacture is lower than that at which degradation occurs. Extrusion and spheronization were the processes used to manufacture TDF pellets as it is a simple and economic approach for production. The effects of extruder and spheronizer speed, amount of spheronization aid and diluents on the pellet size, shape, flow properties and TDF release characteristics were examined. In order to decrease the complexity of analysis and reduce the cost of development a Taguchi orthogonal array design of experiments was successfully applied to evaluate the impact of formulation variables on product characteristics and predict an optimized formulation with a minimum number of experiments. The use of Response Surface Methodology for the development and optimization of pharmaceutical systems, including the optimization of formulation composition, manufacturing processes and/or analytical methods is well established. However the application of RSM requires that accurate, precise and reproducible experimental conditions are used for the generation of reliable data and RSM use is limited due to sensitivity to experimental variability. The benefits of using RSM for formulation optimization include the fact that more than one variable can be investigated at a time and large amounts of information can be generated at the same time ensuring a more efficient process with respect to time and cost. An added advantage of this approach is that mathematical relationships can be generated for the models that are produced and provide formulation scientists with an indication of whether the effect(s) between factors are synergistic or antagonistic. There are several statistical design approaches that use RSM and a Taguchi orthogonal array design was selected for use in this optimization process as fewer experiments are required to generate data for the same number of factors to be investigated when compared to other statistical designs such as Central Composite (CCD) and Box-Behnken designs. The use of RSM clearly demonstrates the impact of different input variables on the % TDF released at 45 min and % TDF loaded into the particles. The amount of sorbitol and Kollidon® CL-M were the only significant variables that affected the % TDF released at 45 min and both excipients had an overall synergistic effect on the in vitro release of TDF. The prediction and manufacture of an optimized formulation led to the production of pellets that met predetermined specifications which was successfully achieved using RSM. The development of a TDF containing pellet dosage form has been achieved and the formulation, manufacture and characterization of the dosage form reveal that the product has the potential to be further developed.
- Full Text:
- Authors: Dube, Tawanda
- Date: 2015
- Language: English
- Type: text , Thesis , Masters , MPharm
- Identifier: http://hdl.handle.net/10962/54690 , vital:26600
- Description: Tenofovir disoproxil fumarate (TDF) is a novel nucleotide analog reverse transcriptase inhibitor that is recommended by the WHO for use in first line treatment of HIV infections. Due to the high dose of TDF for anti-retroviral treatment the formulation of a pellet dosage form may improve patient adherence by incorporation of a large dose in a relatively small dosage form. TDF is currently only available in tablet form. A simple, sensitive, selective, rapid, accurate, precise, stability indicating reversed-phase HPLC method was developed and validated in accordance with ICH guidelines and was successfully used for the analysis of TDF raw material and pharmaceutical dosage forms. Preformulation studies included an investigation of TDF-excipient and excipient-excipient interactions with all materials that could potentially be used to produce extruded and spheronized pellets. Nuclear Magnetic Resonance spectroscopy (NMR), Infrared Spectroscopy (IR), Differential Scanning Colorimetry (DSC) and Thermogravimetric analysis were used for identification and purity testing of TDF and all excipients. DSC data revealed that no potential interactions between TDF and the excipients occurred suggesting that incompatibility reactions were unlikely during manufacture and storage. These findings were confirmed by IR analysis that revealed that no physical interaction was likely between any of the excipients used and TDF. DSC data also reveal the existence of the α and β-polymorphs of TDF as evidenced by two enthalpy changes observed on the resultant thermograms. The existence of two polymorphs is unlikely to result in incompatibility and was confirmed by IR analysis. The IR spectra reveal that all characteristic peaks for TDF were present in 1:1 binary mixtures. Therefore TDF is compatible with all excipients tested and thermal analysis confirmed the stability of TDF under manufacturing conditions. The temperature of degradation temperature established through DSC analysis confirmed that degradation during manufacture is unlikely as the temperature of manufacture is lower than that at which degradation occurs. Extrusion and spheronization were the processes used to manufacture TDF pellets as it is a simple and economic approach for production. The effects of extruder and spheronizer speed, amount of spheronization aid and diluents on the pellet size, shape, flow properties and TDF release characteristics were examined. In order to decrease the complexity of analysis and reduce the cost of development a Taguchi orthogonal array design of experiments was successfully applied to evaluate the impact of formulation variables on product characteristics and predict an optimized formulation with a minimum number of experiments. The use of Response Surface Methodology for the development and optimization of pharmaceutical systems, including the optimization of formulation composition, manufacturing processes and/or analytical methods is well established. However the application of RSM requires that accurate, precise and reproducible experimental conditions are used for the generation of reliable data and RSM use is limited due to sensitivity to experimental variability. The benefits of using RSM for formulation optimization include the fact that more than one variable can be investigated at a time and large amounts of information can be generated at the same time ensuring a more efficient process with respect to time and cost. An added advantage of this approach is that mathematical relationships can be generated for the models that are produced and provide formulation scientists with an indication of whether the effect(s) between factors are synergistic or antagonistic. There are several statistical design approaches that use RSM and a Taguchi orthogonal array design was selected for use in this optimization process as fewer experiments are required to generate data for the same number of factors to be investigated when compared to other statistical designs such as Central Composite (CCD) and Box-Behnken designs. The use of RSM clearly demonstrates the impact of different input variables on the % TDF released at 45 min and % TDF loaded into the particles. The amount of sorbitol and Kollidon® CL-M were the only significant variables that affected the % TDF released at 45 min and both excipients had an overall synergistic effect on the in vitro release of TDF. The prediction and manufacture of an optimized formulation led to the production of pellets that met predetermined specifications which was successfully achieved using RSM. The development of a TDF containing pellet dosage form has been achieved and the formulation, manufacture and characterization of the dosage form reveal that the product has the potential to be further developed.
- Full Text:
FPGA Based Implementation of a High Performance Scalable NetFlow Filter
- Herbert, Alan, Irwin, Barry V W, Otten, D F, Balmahoon, M R
- Authors: Herbert, Alan , Irwin, Barry V W , Otten, D F , Balmahoon, M R
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/427887 , vital:72470 , https://www.researchgate.net/profile/Barry-Ir-win/publication/327622948_FPGA_Based_Implementation_of_a_High_Perfor-mance_Scalable_NetFlow_Filter/links/5b9a17a192851c4ba8181ba5/FPGA-Based-Implementation-of-a-High-Performance-Scalable-NetFlow-Filter.pdf
- Description: Full packet analysis on firewalls and intrusion detection, although effec-tive, has been found in recent times to be detrimental to the overall per-formance of networks that receive large volumes of throughput. For this reason partial packet analysis algorithms such as the NetFlow protocol have emerged to better mitigate these bottlenecks. This research delves into implementing a hardware accelerated, scalable, high per-formance system for NetFlow analysis and attack mitigation. Further-more, this implementation takes on attack mitigation through collection and processing of network flows produced at the source, rather than at the site of incident. This research platform manages to scale out its back-end through dis-tributed analysis over multiple hosts using the ZeroMQ toolset. Fur-thermore, ZeroMQ allows for multiple NetFlow data publishers, so that plug-ins can subscribe to the publishers that contain the relevant data to further increase the overall performance of the system. The dedicat-ed custom hardware optimizes the received network flows through cleaning, summarization and re-ordering into an easy to pass form when given to the sequential component of the system; this being the back-end.
- Full Text:
- Authors: Herbert, Alan , Irwin, Barry V W , Otten, D F , Balmahoon, M R
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/427887 , vital:72470 , https://www.researchgate.net/profile/Barry-Ir-win/publication/327622948_FPGA_Based_Implementation_of_a_High_Perfor-mance_Scalable_NetFlow_Filter/links/5b9a17a192851c4ba8181ba5/FPGA-Based-Implementation-of-a-High-Performance-Scalable-NetFlow-Filter.pdf
- Description: Full packet analysis on firewalls and intrusion detection, although effec-tive, has been found in recent times to be detrimental to the overall per-formance of networks that receive large volumes of throughput. For this reason partial packet analysis algorithms such as the NetFlow protocol have emerged to better mitigate these bottlenecks. This research delves into implementing a hardware accelerated, scalable, high per-formance system for NetFlow analysis and attack mitigation. Further-more, this implementation takes on attack mitigation through collection and processing of network flows produced at the source, rather than at the site of incident. This research platform manages to scale out its back-end through dis-tributed analysis over multiple hosts using the ZeroMQ toolset. Fur-thermore, ZeroMQ allows for multiple NetFlow data publishers, so that plug-ins can subscribe to the publishers that contain the relevant data to further increase the overall performance of the system. The dedicat-ed custom hardware optimizes the received network flows through cleaning, summarization and re-ordering into an easy to pass form when given to the sequential component of the system; this being the back-end.
- Full Text:
Free space in the academy
- Authors: Janz, Bruce B
- Date: 2015
- Subjects: Academic Freedom -- South Africa Universities and colleges -- South Africa Equality Liberty Education and state -- South Africa Education, Higher -- South Africa
- Language: English
- Type: text
- Identifier: http://hdl.handle.net/10962/848 , vital:19996
- Description: “Academic freedom” does not mean the same thing to everyone. There are, to be sure, a few who argue against it. Sandra Korn, in the Harvard Crimson last year, argued that we should abandon academic freedom in favor of academic justice. She argues that we have reached a consensus on issues such as racism, classism and sexism, and so to promote racist or classist or sexist views under the guise of “academic freedom” is to ignore a higher standard and more importantly to ignore the fact that academic freedom is always couched in political realities, and is never the dispassionate exercise of reason and the pursuit of knowledge. It always serves an agenda, and so if that is the case, it should serve the agenda of justice, particularly justice for disadvantaged and marginalized people. Her online article, when I last looked, had almost 1300 comments, and had inspired commentary from a number of other publications. You can imagine the range of these comments and reactions: everything from “this is long overdue” to the newest favorite insult circulating the internet, “she’s just another Social Justice Warrior (SJW).”
- Full Text:
- Authors: Janz, Bruce B
- Date: 2015
- Subjects: Academic Freedom -- South Africa Universities and colleges -- South Africa Equality Liberty Education and state -- South Africa Education, Higher -- South Africa
- Language: English
- Type: text
- Identifier: http://hdl.handle.net/10962/848 , vital:19996
- Description: “Academic freedom” does not mean the same thing to everyone. There are, to be sure, a few who argue against it. Sandra Korn, in the Harvard Crimson last year, argued that we should abandon academic freedom in favor of academic justice. She argues that we have reached a consensus on issues such as racism, classism and sexism, and so to promote racist or classist or sexist views under the guise of “academic freedom” is to ignore a higher standard and more importantly to ignore the fact that academic freedom is always couched in political realities, and is never the dispassionate exercise of reason and the pursuit of knowledge. It always serves an agenda, and so if that is the case, it should serve the agenda of justice, particularly justice for disadvantaged and marginalized people. Her online article, when I last looked, had almost 1300 comments, and had inspired commentary from a number of other publications. You can imagine the range of these comments and reactions: everything from “this is long overdue” to the newest favorite insult circulating the internet, “she’s just another Social Justice Warrior (SJW).”
- Full Text:
From linguistic determinism to technological determinism
- Kaschula, Russell H, Mostert, André M
- Authors: Kaschula, Russell H , Mostert, André M
- Date: 2015
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/67062 , vital:29027 , https://doi.org/10.4018/978-1-4666-5888-2.ch447
- Description: publisher version , From Introduction: This article seeks to analyse the link between linguistic determinism, the notion that language determines our thought and the way we perceive our reality, as espoused by the Sapir-Whorf hypothesis, and contemporary technological determinism. Arguably this link takes place within a global context where equal access to technology is not yet guaranteed. Ellul (1964) and Lawson’s (2004) observations create an interesting metaphor in terms of the technological beast staring down human society. The overwhelming response if we accept Lawson’s observation is complacency at best or downright naivety at worst regarding the impact of technology on our thinking. Lawson made his observation in the pre-Facebook era and subsequent literature is now much more focused on all aspects of technology within our contemporary milieu. Technology is now totally ubiquitous in the developed world and becoming more so in developing countries, albeit with a much stronger mobile bias for early technology adopters. That technology is rushing ahead of many individuals and institutions is almost an aphorism with many lagging in its wake. This lag as recognised by Brynjolfson and McAfree (2011) has wide social and economic implications for all members of society, in the case of business those that do not keep up go under. A further pertinent question revolves around how individuals who start from low technological literacy levels or do not keep pace with technological developments are impacted. Dlutu (2013) assesses for example the impact of social network sites on the isiXhosa language and culture in both a rural and urban area of South Africa. Furthermore one may then ask how this technological milieu impacts on the general aspects of the day to day lives and thinking of all members of a society. This gives rise to the concept of technological determinism which in its simplest form states ‘that technology has important effects on our lives’ (Adler, 2008, p. 537). This is far too simplistic when the contemporaneous technological developments are assessed. Adler (2008, p. 537) goes further and recognizes ‘that technology itself is socially determined…and social structures co-evolve in a non-deterministic, emergent process…the effects of any given technology depend mainly on how it is implemented which in turn is socially determined’ (Adler, 2008, p. 537). Engaging this socially determined application of technology can be advanced when the relationship with language and language determinism is considered. Moreover, it is the interplay between technology and language that gives rise to the emerging concept of a language singularity which is brought about by a form of technological determinism. This article seeks to explore the possible effects of rapid technological development on human interaction, language and culture in a ‘globalized’ world which has unequal access to literacy and technology. Furthermore, the article explores the link between language, culture, thought and technology and the type of linguistic and technological determinism that we can anticipate.
- Full Text: false
- Authors: Kaschula, Russell H , Mostert, André M
- Date: 2015
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/67062 , vital:29027 , https://doi.org/10.4018/978-1-4666-5888-2.ch447
- Description: publisher version , From Introduction: This article seeks to analyse the link between linguistic determinism, the notion that language determines our thought and the way we perceive our reality, as espoused by the Sapir-Whorf hypothesis, and contemporary technological determinism. Arguably this link takes place within a global context where equal access to technology is not yet guaranteed. Ellul (1964) and Lawson’s (2004) observations create an interesting metaphor in terms of the technological beast staring down human society. The overwhelming response if we accept Lawson’s observation is complacency at best or downright naivety at worst regarding the impact of technology on our thinking. Lawson made his observation in the pre-Facebook era and subsequent literature is now much more focused on all aspects of technology within our contemporary milieu. Technology is now totally ubiquitous in the developed world and becoming more so in developing countries, albeit with a much stronger mobile bias for early technology adopters. That technology is rushing ahead of many individuals and institutions is almost an aphorism with many lagging in its wake. This lag as recognised by Brynjolfson and McAfree (2011) has wide social and economic implications for all members of society, in the case of business those that do not keep up go under. A further pertinent question revolves around how individuals who start from low technological literacy levels or do not keep pace with technological developments are impacted. Dlutu (2013) assesses for example the impact of social network sites on the isiXhosa language and culture in both a rural and urban area of South Africa. Furthermore one may then ask how this technological milieu impacts on the general aspects of the day to day lives and thinking of all members of a society. This gives rise to the concept of technological determinism which in its simplest form states ‘that technology has important effects on our lives’ (Adler, 2008, p. 537). This is far too simplistic when the contemporaneous technological developments are assessed. Adler (2008, p. 537) goes further and recognizes ‘that technology itself is socially determined…and social structures co-evolve in a non-deterministic, emergent process…the effects of any given technology depend mainly on how it is implemented which in turn is socially determined’ (Adler, 2008, p. 537). Engaging this socially determined application of technology can be advanced when the relationship with language and language determinism is considered. Moreover, it is the interplay between technology and language that gives rise to the emerging concept of a language singularity which is brought about by a form of technological determinism. This article seeks to explore the possible effects of rapid technological development on human interaction, language and culture in a ‘globalized’ world which has unequal access to literacy and technology. Furthermore, the article explores the link between language, culture, thought and technology and the type of linguistic and technological determinism that we can anticipate.
- Full Text: false
Gay men and fatherhood in South Africa: a discursive study
- Morison, Tracy, Lynch, Ingrid, Reddy, Vasu
- Authors: Morison, Tracy , Lynch, Ingrid , Reddy, Vasu
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/143782 , vital:38282 , https://ischp.files.wordpress.com/2015/08/ischp_2015_abstract_booklet.pdf
- Description: There is little South African research on gender and sexual minorities’ reproductive decision-making and, to date, no published work explicitly focused on gay men. Motivated by the virtual absence of gay men in research, as well as their marginalisation more generally, we undertook a qualitative investigation of gay men’s thoughts, feelings and perspectives of fatherhood, fatherhood decisions, and experiences of pathways to parenthood. Framed by a reproductive justice perspective, the aim of the study was not only to generate new knowledge, but also to inform policy, services, and advocacy. In this paper we present some of the findings from our discursive analysis of participants’ accounts of their own experiences of the pathway to parenthood or remaining ‘childfree’. We locate our analysis within the broader South African context and show how the entanglement of various social identity markers - particularly gender, race, and class - come to bear on participants’ experiences.
- Full Text:
- Authors: Morison, Tracy , Lynch, Ingrid , Reddy, Vasu
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/143782 , vital:38282 , https://ischp.files.wordpress.com/2015/08/ischp_2015_abstract_booklet.pdf
- Description: There is little South African research on gender and sexual minorities’ reproductive decision-making and, to date, no published work explicitly focused on gay men. Motivated by the virtual absence of gay men in research, as well as their marginalisation more generally, we undertook a qualitative investigation of gay men’s thoughts, feelings and perspectives of fatherhood, fatherhood decisions, and experiences of pathways to parenthood. Framed by a reproductive justice perspective, the aim of the study was not only to generate new knowledge, but also to inform policy, services, and advocacy. In this paper we present some of the findings from our discursive analysis of participants’ accounts of their own experiences of the pathway to parenthood or remaining ‘childfree’. We locate our analysis within the broader South African context and show how the entanglement of various social identity markers - particularly gender, race, and class - come to bear on participants’ experiences.
- Full Text:
Genetic and biological characterisation of a novel Plutella xylostella granulovirus, PlxyGV-SA
- Abdulkadir, Fatima, Knox, Caroline M, Marsberg, Tamryn, Hill, Martin P, Moore, Sean D
- Authors: Abdulkadir, Fatima , Knox, Caroline M , Marsberg, Tamryn , Hill, Martin P , Moore, Sean D
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/417971 , vital:71498 , xlink:href="https://doi.org/10.1007/s10526-015-9666-3"
- Description: Plutella xylostella granulovirus (PlxyGV) has been isolated from insect populations in many countries and is considered a potential biopesticide for sustainable control of P. xylostella (L.) (Lepidoptera: Plutellidae). Several PlxyGV isolates have been genetically characterised, and the full genome sequence of PlxyGV-Japan is available for comparison with novel isolates. A South African PlxyGV was recently recovered from an overcrowded laboratory P. xylostella colony and identified as a genetically distinct isolate by sequencing of the granulin gene and restriction endonuclease (REN) analysis of genomic DNA. In this report, PlxyGV-SA was further characterised by PCR amplification and sequencing of egt, lef-8 and lef-9 genes, and several amino acid substitutions were observed. The PstI REN profile of PlxyGV-SA was different from that of PlxyGV-Japan in terms of band size and number, thereby confirming its novel genetic identity. Surface dose bioassays showed that PlxyGV-SA is pathogenic to neonate but not late instar larvae at the same and higher virus doses, indicating that a biopesticide should be targeted at early larval stages in the field.
- Full Text:
- Authors: Abdulkadir, Fatima , Knox, Caroline M , Marsberg, Tamryn , Hill, Martin P , Moore, Sean D
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/417971 , vital:71498 , xlink:href="https://doi.org/10.1007/s10526-015-9666-3"
- Description: Plutella xylostella granulovirus (PlxyGV) has been isolated from insect populations in many countries and is considered a potential biopesticide for sustainable control of P. xylostella (L.) (Lepidoptera: Plutellidae). Several PlxyGV isolates have been genetically characterised, and the full genome sequence of PlxyGV-Japan is available for comparison with novel isolates. A South African PlxyGV was recently recovered from an overcrowded laboratory P. xylostella colony and identified as a genetically distinct isolate by sequencing of the granulin gene and restriction endonuclease (REN) analysis of genomic DNA. In this report, PlxyGV-SA was further characterised by PCR amplification and sequencing of egt, lef-8 and lef-9 genes, and several amino acid substitutions were observed. The PstI REN profile of PlxyGV-SA was different from that of PlxyGV-Japan in terms of band size and number, thereby confirming its novel genetic identity. Surface dose bioassays showed that PlxyGV-SA is pathogenic to neonate but not late instar larvae at the same and higher virus doses, indicating that a biopesticide should be targeted at early larval stages in the field.
- Full Text:
Global Cicada Sound Collection I: Recordings from South Africa and Malawi by BW Price and MH Villet and harvesting of BioAcoustica data by GBIF
- Baker, Ed, Price, Benjamin W, Rycroft, Simon, Villet, Martin H
- Authors: Baker, Ed , Price, Benjamin W , Rycroft, Simon , Villet, Martin H
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/441420 , vital:73886 , https://bdj.pensoft.net/articles.php?id=5792
- Description: Sound collections for singing insects provide important repositories that underpin existing research (eg Price et al. 2007 at http://bio. acousti. ca/node/11801; Price et al. 2010) and make bioacoustic collections available for future work, including insect communication (Ordish 1992), systematics (eg David et al. 2003), and automated identification (Bennett et al. 2015). The BioAcoustica platform (Baker et al. 2015) is both a repository and analysis platform for bioacoustic collections: allowing collections to be available in perpetuity, and also facilitating complex analyses using the BioVeL cloud infrastructure (Vicario et al. 2011). The Global Cicada Sound Collection is a project to make recordings of the world's cicadas (Hemiptera: Cicadidae) available using open licences to maximise their potential for study and reuse. This first component of the Global Cicada Sound Collection comprises recordings made between 2006 and 2008 of Cicadidae in South Africa and Malawi.
- Full Text:
- Authors: Baker, Ed , Price, Benjamin W , Rycroft, Simon , Villet, Martin H
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/441420 , vital:73886 , https://bdj.pensoft.net/articles.php?id=5792
- Description: Sound collections for singing insects provide important repositories that underpin existing research (eg Price et al. 2007 at http://bio. acousti. ca/node/11801; Price et al. 2010) and make bioacoustic collections available for future work, including insect communication (Ordish 1992), systematics (eg David et al. 2003), and automated identification (Bennett et al. 2015). The BioAcoustica platform (Baker et al. 2015) is both a repository and analysis platform for bioacoustic collections: allowing collections to be available in perpetuity, and also facilitating complex analyses using the BioVeL cloud infrastructure (Vicario et al. 2011). The Global Cicada Sound Collection is a project to make recordings of the world's cicadas (Hemiptera: Cicadidae) available using open licences to maximise their potential for study and reuse. This first component of the Global Cicada Sound Collection comprises recordings made between 2006 and 2008 of Cicadidae in South Africa and Malawi.
- Full Text:
Global Handbook of Quality of Life: exploration of well-being of nations and continents
- Glatzer, Wolfgang, Camfield, Laura, Moller, Valerie, Rojas, Mariano
- Authors: Glatzer, Wolfgang , Camfield, Laura , Moller, Valerie , Rojas, Mariano
- Date: 2015
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/67245 , vital:29063 , https://doi.org/10.1007/978-94-017-9178-6
- Description: publisher version , This handbook provides a comprehensive historical account of the field of Quality of Life. It brings together theoretical insights and empirical findings, and presents the main items of global quality of life and wellbeing research. Worldwide in its scope of topics, the handbook examines discussions of demographic and health development, the spread of democracy, global economic accounting, multi-item measurement of perceived satisfaction and expert-assessed quality of life, and the well-being of children, women and poor people. It looks at well-being in specific regions, including North and Sub-Saharan Africa, Asia, South America, and Eastern and Western Europe. In addition to contributions by leading and younger authors, the handbook includes contributions from International Organizations about their own work with respect to social reporting.
- Full Text: false
- Authors: Glatzer, Wolfgang , Camfield, Laura , Moller, Valerie , Rojas, Mariano
- Date: 2015
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/67245 , vital:29063 , https://doi.org/10.1007/978-94-017-9178-6
- Description: publisher version , This handbook provides a comprehensive historical account of the field of Quality of Life. It brings together theoretical insights and empirical findings, and presents the main items of global quality of life and wellbeing research. Worldwide in its scope of topics, the handbook examines discussions of demographic and health development, the spread of democracy, global economic accounting, multi-item measurement of perceived satisfaction and expert-assessed quality of life, and the well-being of children, women and poor people. It looks at well-being in specific regions, including North and Sub-Saharan Africa, Asia, South America, and Eastern and Western Europe. In addition to contributions by leading and younger authors, the handbook includes contributions from International Organizations about their own work with respect to social reporting.
- Full Text: false
Guarded visions: walls, watchtowers and warped perspectives in the Israeli occupied West Bank Palestinian territory
- Authors: Baasch, Rachel M
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/147281 , vital:38611 , https://0-hdl.handle.net.wam.seals.ac.za/10520/EJC176318
- Description: This paper examines the relationship between Israel's fortification of physical space and narratives of division in the Israeli occupied Palestinian West Bank Territory. I argue that the fortification and separation of physical space deepens segregation, and increases fear, hostility and disconnection between people living in this context. Furthermore, I suggest that this relationship between narratives of division and insecurity and structural mechanisms of control within the West Bank influences and impacts on individuals such that personal perspectives become guarded and defensive. The mediation of subjects through a defensive lens can prevent individuals from forming connections that acknowledge the permeability of seemingly impenetrable distinctions between inside and outside, or self and an-other. The looking, recording and representation of people in a place that is guarded and framed from a position of insecurity reduces the capacity of individuals to locate openings that traverse restrictive boundaries. In order to contextualise my discussion, I have included personal documentation of defensive structures photographed in the West Bank between 2013 and 2014. I position my observations and analyses in relation to discussions about the Oush Grab Military Base presented by the Decolonizing Architecture Art Residency (DAAR) in their recent publication Architecture after revolution (2013).
- Full Text:
- Authors: Baasch, Rachel M
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/147281 , vital:38611 , https://0-hdl.handle.net.wam.seals.ac.za/10520/EJC176318
- Description: This paper examines the relationship between Israel's fortification of physical space and narratives of division in the Israeli occupied Palestinian West Bank Territory. I argue that the fortification and separation of physical space deepens segregation, and increases fear, hostility and disconnection between people living in this context. Furthermore, I suggest that this relationship between narratives of division and insecurity and structural mechanisms of control within the West Bank influences and impacts on individuals such that personal perspectives become guarded and defensive. The mediation of subjects through a defensive lens can prevent individuals from forming connections that acknowledge the permeability of seemingly impenetrable distinctions between inside and outside, or self and an-other. The looking, recording and representation of people in a place that is guarded and framed from a position of insecurity reduces the capacity of individuals to locate openings that traverse restrictive boundaries. In order to contextualise my discussion, I have included personal documentation of defensive structures photographed in the West Bank between 2013 and 2014. I position my observations and analyses in relation to discussions about the Oush Grab Military Base presented by the Decolonizing Architecture Art Residency (DAAR) in their recent publication Architecture after revolution (2013).
- Full Text:
Health Psychology and the framing of abortion in Africa: a critical review of the literature
- Macleod, Catriona I, Chiweshe, Malvern T, Mavuso, Jabulile M-J J
- Authors: Macleod, Catriona I , Chiweshe, Malvern T , Mavuso, Jabulile M-J J
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/143871 , vital:38290 , https://ischp.files.wordpress.com/2015/08/ischp_2015_abstract_booklet.pdf
- Description: Despite 97% of abortions performed in Africa being classifiable as unsafe, there has been virtually no engagement in knowledge production about abortion in Africa from psychologists, outside of South Africa. Taking a feminist health psychology approach, we conducted a systematic review of published research on this topic featured in PsycINFO over a six year period. We analysed the 39 articles included in the review in terms of countries in which the research was conducted, types of research, issues covered, framings, and main findings. The results show that apart from a public health framing, perspectives that foreground contextual, social, cultural, gendered perspectives dominate. While abortion services, unsafe abortion and the incidence of abortion were well researched, so too were attitudes and public discourses on abortion. Clinical psychological, reproductive justice or rights and medical framings received little attention. We outline the implications of this knowledge base for feminist health psychology in Africa.
- Full Text:
- Authors: Macleod, Catriona I , Chiweshe, Malvern T , Mavuso, Jabulile M-J J
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/143871 , vital:38290 , https://ischp.files.wordpress.com/2015/08/ischp_2015_abstract_booklet.pdf
- Description: Despite 97% of abortions performed in Africa being classifiable as unsafe, there has been virtually no engagement in knowledge production about abortion in Africa from psychologists, outside of South Africa. Taking a feminist health psychology approach, we conducted a systematic review of published research on this topic featured in PsycINFO over a six year period. We analysed the 39 articles included in the review in terms of countries in which the research was conducted, types of research, issues covered, framings, and main findings. The results show that apart from a public health framing, perspectives that foreground contextual, social, cultural, gendered perspectives dominate. While abortion services, unsafe abortion and the incidence of abortion were well researched, so too were attitudes and public discourses on abortion. Clinical psychological, reproductive justice or rights and medical framings received little attention. We outline the implications of this knowledge base for feminist health psychology in Africa.
- Full Text:
Historical demography of southern African patellid limpets: congruence of population expansions, but not phylogeography
- Mmonwa, Kolobe L, Teske, Peter R, McQuaid, Christopher D, Barker, Nigel P
- Authors: Mmonwa, Kolobe L , Teske, Peter R , McQuaid, Christopher D , Barker, Nigel P
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/444814 , vital:74300 , https://doi.org/10.2989/1814232X.2015.1009165
- Description: Global climatic oscillations have shaped the contemporary genetic structure of marine taxa in different ways. Previous demographic studies have indicated that various intertidal marine species display genetic signatures of demographic expansion that either pre- or postdate the Last Glacial Maximum. Such expansions and the ability of species to colonise new habitats will influence their genetic structure, but the link between scales of larval dispersal and the strength of phylogeographic structure is not always clear. We analysed a fragment of the mitochondrial COI gene of 11 sympatric species of intertidal southern African patellid limpets to investigate how ancient oceanographic dynamics have shaped and maintained their contemporary spatial genetic variation. Our data show that the patellid limpets investigated display congruent evidence of spatial expansion during the Late Pleistocene or Early Holocene, which corresponds with the establishment of the contemporary southern African shoreline. We argue that closely related and co-distributed southern African intertidal invertebrates responded to ancient climatic oscillations as a cohesive group. In contrast, contemporary oceanographic circulation has shaped the phylogeographic patterns of these limpets in different ways. We show close relationships between phylogeography and biogeography for some species, but not for others, despite the similarities in their life histories and exposure to the same climatic changes.
- Full Text:
- Authors: Mmonwa, Kolobe L , Teske, Peter R , McQuaid, Christopher D , Barker, Nigel P
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/444814 , vital:74300 , https://doi.org/10.2989/1814232X.2015.1009165
- Description: Global climatic oscillations have shaped the contemporary genetic structure of marine taxa in different ways. Previous demographic studies have indicated that various intertidal marine species display genetic signatures of demographic expansion that either pre- or postdate the Last Glacial Maximum. Such expansions and the ability of species to colonise new habitats will influence their genetic structure, but the link between scales of larval dispersal and the strength of phylogeographic structure is not always clear. We analysed a fragment of the mitochondrial COI gene of 11 sympatric species of intertidal southern African patellid limpets to investigate how ancient oceanographic dynamics have shaped and maintained their contemporary spatial genetic variation. Our data show that the patellid limpets investigated display congruent evidence of spatial expansion during the Late Pleistocene or Early Holocene, which corresponds with the establishment of the contemporary southern African shoreline. We argue that closely related and co-distributed southern African intertidal invertebrates responded to ancient climatic oscillations as a cohesive group. In contrast, contemporary oceanographic circulation has shaped the phylogeographic patterns of these limpets in different ways. We show close relationships between phylogeography and biogeography for some species, but not for others, despite the similarities in their life histories and exposure to the same climatic changes.
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Host searching and oviposition behaviour of Agathis bishopi (Hymenoptera: Braconidae), a larval parasitoid of false codling moth, Thaumatotibia leucotreta (Lepidoptera: Tortricidae): a potential proxy indicator for fruit infestation
- Zimba, Kennedy J, Heshula, Lelethu U P, Moore, Sean D, Hill, Martin P
- Authors: Zimba, Kennedy J , Heshula, Lelethu U P , Moore, Sean D , Hill, Martin P
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/407019 , vital:70330 , xlink:href="https://hdl.handle.net/10520/EJC195073"
- Description: Agathis bishopi (Nixon) (Hymenoptera: Braconidae)is an arrhenotokous larval endoparasitoid of Thaumatotibia leucotreta (Meyrick) (Lepidoptera: Tortricidae) (Gendall 2007; Hofmeyr et al. 2015) commonly known as false codling moth (FCM), a major pest of citrus in South Africa (Moore et al. 2004; Malan et al. 2011). Under field conditions, A. bishopi was identified attacking more than 34% of FCM larvae in fruit, showing good biocontrol potential (Gendall 2007). Preference by A. bishopi for parasitising the early instars of its concealed host suggests that the parasitoid has strong natural host location ability (Sishuba 2003; Gendall 2007).
- Full Text:
- Authors: Zimba, Kennedy J , Heshula, Lelethu U P , Moore, Sean D , Hill, Martin P
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/407019 , vital:70330 , xlink:href="https://hdl.handle.net/10520/EJC195073"
- Description: Agathis bishopi (Nixon) (Hymenoptera: Braconidae)is an arrhenotokous larval endoparasitoid of Thaumatotibia leucotreta (Meyrick) (Lepidoptera: Tortricidae) (Gendall 2007; Hofmeyr et al. 2015) commonly known as false codling moth (FCM), a major pest of citrus in South Africa (Moore et al. 2004; Malan et al. 2011). Under field conditions, A. bishopi was identified attacking more than 34% of FCM larvae in fruit, showing good biocontrol potential (Gendall 2007). Preference by A. bishopi for parasitising the early instars of its concealed host suggests that the parasitoid has strong natural host location ability (Sishuba 2003; Gendall 2007).
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How can conversational analysis contribute to ‘doing’ critical work?: extending the methodological conversation
- Authors: du Toit, Ryan
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/143904 , vital:38293 , https://ischp.files.wordpress.com/2015/08/ischp_2015_abstract_booklet.pdf
- Description: Conversation analysis (CA) has been criticised as being overly obsessed with the intricate details of conversation and bracketing off the wider socio-structural power relations in which conversation is embedded. Many scholars regard CA as a methodology that is incompatible with, and thus incapable of doing, critical work. This paper seeks to contribute to the methodological conversation by proposing a dual analytical methodology that merges understandings of talk from CA and critical discursive approaches, and attends to both the conversational (turn-by-turn) context as well as the discursive resources that inhabit talk. This is accomplished through a triangulatory activity that takes different conceptualizations of ‘context’ into consideration and investigates how subject positions are worked up on a micro- (turnby-turn) and macro-level. The authors illustrate this approach using institutional talk collected at a UK diabetes clinic and from interviews investigating how employers talk about their domestic workers in South Africa.
- Full Text:
- Authors: du Toit, Ryan
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/143904 , vital:38293 , https://ischp.files.wordpress.com/2015/08/ischp_2015_abstract_booklet.pdf
- Description: Conversation analysis (CA) has been criticised as being overly obsessed with the intricate details of conversation and bracketing off the wider socio-structural power relations in which conversation is embedded. Many scholars regard CA as a methodology that is incompatible with, and thus incapable of doing, critical work. This paper seeks to contribute to the methodological conversation by proposing a dual analytical methodology that merges understandings of talk from CA and critical discursive approaches, and attends to both the conversational (turn-by-turn) context as well as the discursive resources that inhabit talk. This is accomplished through a triangulatory activity that takes different conceptualizations of ‘context’ into consideration and investigates how subject positions are worked up on a micro- (turnby-turn) and macro-level. The authors illustrate this approach using institutional talk collected at a UK diabetes clinic and from interviews investigating how employers talk about their domestic workers in South Africa.
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How general-purpose can a GPU be?
- Authors: Machanick, Philip
- Date: 2015
- Language: English
- Type: article , text
- Identifier: http://hdl.handle.net/10962/61180 , vital:27988 , http://dx.doi.org/10.18489/sacj.v0i57.347
- Description: The use of graphics processing units (GPUs) in general-purpose computation (GPGPU) is a growing field. GPU instruction sets, while implementing a graphics pipeline, draw from a range of single instruction multiple datastream (SIMD) architectures characteristic of the heyday of supercomputers. Yet only one of these SIMD instruction sets has been of application on a wide enough range of problems to survive the era when the full range of supercomputer design variants was being explored: vector instructions. Supercomputers covered a range of exotic designs such as hypercubes and the Connection Machine (Fox, 1989). The latter is likely the source of the snide comment by Cray: it had thousands of relatively low-speed CPUs (Tucker & Robertson, 1988). Since Cray won, why are we not basing our ideas on his designs (Cray Inc., 2004), rather than those of the losers? The Top 500 supercomputer list is dominated by general-purpose CPUs, and nothing like the Connection Machine that headed the list in 1993 still exists.
- Full Text:
- Authors: Machanick, Philip
- Date: 2015
- Language: English
- Type: article , text
- Identifier: http://hdl.handle.net/10962/61180 , vital:27988 , http://dx.doi.org/10.18489/sacj.v0i57.347
- Description: The use of graphics processing units (GPUs) in general-purpose computation (GPGPU) is a growing field. GPU instruction sets, while implementing a graphics pipeline, draw from a range of single instruction multiple datastream (SIMD) architectures characteristic of the heyday of supercomputers. Yet only one of these SIMD instruction sets has been of application on a wide enough range of problems to survive the era when the full range of supercomputer design variants was being explored: vector instructions. Supercomputers covered a range of exotic designs such as hypercubes and the Connection Machine (Fox, 1989). The latter is likely the source of the snide comment by Cray: it had thousands of relatively low-speed CPUs (Tucker & Robertson, 1988). Since Cray won, why are we not basing our ideas on his designs (Cray Inc., 2004), rather than those of the losers? The Top 500 supercomputer list is dominated by general-purpose CPUs, and nothing like the Connection Machine that headed the list in 1993 still exists.
- Full Text:
How General-Purpose can a GPU be?
- Authors: Machanick, Philip
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/439294 , vital:73563 , https://hdl.handle.net/10520/EJC181693
- Description: The use of graphics processing units (GPUs) in general-purpose computation (GPGPU) is a growing field. GPU instruction sets, while implementing a graphics pipeline, draw from a range of single instruction multiple data stream (SIMD) architectures characteristic of the heyday of supercomputers. Yet only one of these SIMD instruction sets has been of application on a wide enough range of problems to survive the era when the full range of supercomputer design variants was being explored: vector instructions.
- Full Text:
- Authors: Machanick, Philip
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/439294 , vital:73563 , https://hdl.handle.net/10520/EJC181693
- Description: The use of graphics processing units (GPUs) in general-purpose computation (GPGPU) is a growing field. GPU instruction sets, while implementing a graphics pipeline, draw from a range of single instruction multiple data stream (SIMD) architectures characteristic of the heyday of supercomputers. Yet only one of these SIMD instruction sets has been of application on a wide enough range of problems to survive the era when the full range of supercomputer design variants was being explored: vector instructions.
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Hsp70/Hsp90 organising protein (hop): beyond interactions with chaperones and prion proteins
- Baindur-Hudson, Swati, Edkins, Adrienne L, Blatch, Gregory L
- Authors: Baindur-Hudson, Swati , Edkins, Adrienne L , Blatch, Gregory L
- Date: 2015
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/164852 , vital:41178 , ISBN 978-3-319-11730-0 , DOI: 10.1007/978-3-319-11731-7_3
- Description: The Hsp70/Hsp90 organising protein (Hop), also known as stress-inducible protein 1 (STI1), has received considerable attention for diverse cellular functions in both healthy and diseased states. There is extensive evidence that intracellular Hop is a co-chaperone of the major chaperones Hsp70 and Hsp90, playing an important role in the productive folding of Hsp90 client proteins. Consequently, Hop is implicated in a number of key signalling pathways, including aberrant pathways leading to cancer. However, Hop is also secreted and it is now well established that Hop also serves as a receptor for the prion protein, PrPC.
- Full Text:
- Authors: Baindur-Hudson, Swati , Edkins, Adrienne L , Blatch, Gregory L
- Date: 2015
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/164852 , vital:41178 , ISBN 978-3-319-11730-0 , DOI: 10.1007/978-3-319-11731-7_3
- Description: The Hsp70/Hsp90 organising protein (Hop), also known as stress-inducible protein 1 (STI1), has received considerable attention for diverse cellular functions in both healthy and diseased states. There is extensive evidence that intracellular Hop is a co-chaperone of the major chaperones Hsp70 and Hsp90, playing an important role in the productive folding of Hsp90 client proteins. Consequently, Hop is implicated in a number of key signalling pathways, including aberrant pathways leading to cancer. However, Hop is also secreted and it is now well established that Hop also serves as a receptor for the prion protein, PrPC.
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Human FN1 is regulated by the heat-shock response
- Authors: Dhanani, Karim Colin Hassan
- Date: 2015
- Subjects: Uncatalogued
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/193487 , vital:45336
- Description: Heat shock protein 90 (Hsp90) and heat shock factors (HSFs) are known to be involved in the epigenetic regulation of several fundamental oncogenic genes. Fibronectin (FN) is an extracellular matrix (ECM) glycoprotein which plays key roles in cell adhesion and migration. Hsp90 binds directly to FN and Hsp90 inhibition has been shown to regulate FN protein levels and matrix formation. Where inhibition of Hsp90 with a C-terminal inhibitor (novobiocin) induced the loss of FN matrix, treatment with an N-terminal inhibitor (geldanamycin) increased FN matrix levels. GA treatment induced a strong dose and time dependent increase in FN1 promoter activity and increased total FN mRNA respectively. By contrast, NOV showed no increase in the promoter activity and no change in the expression of FN mRNA. As GA is known to induce the stress response, we investigated the relationship between the cell stress machinery and the transcriptional regulation of FN. Three putative heat shock elements (HSEs) were identified in the FN1 promoter. The loss of two of the three identified putative HSEs resulted in a loss in the basal transcriptional activity of the FN1 promoter in our reporter model. This was in addition to the loss of the induction of transcriptional activity with GA treatment observed with the full-length promoter. Binding of HSF1 to one of the putative HSEs, which was identified as potentially functional from the truncation analysis, was confirmed using ChIP. The occupancy of this HSE by HSF1 was shown to increase with GA treatment. These data support the hypothesis that FN1 is a functional HSF1 target gene. The 5' promoter regions of seven additional ECM protein encoding genes were analysed and mRNA levels were detected by quantitative RT-PCR upon treatment with GA. Collagen 4 _2 and laminin _3 mRNA were found to increase in the presence of GA, whereas collagen 4 _3 and osteopontin showed no change. Similarly to FN1, these data indicate that a subset of ECM genes may be under the regulation of the HSF1 mediated heat-shock response. This may have implications for our understanding of ECM dynamics in cancer, where the clinical application of Hsp90 inhibitors is intended. Additionally, our data provide a poten- tial underpinning for the role of the HSF1 mediated heat-shock response in several fibrotic and metabolic stress related pathologies. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2015
- Full Text:
- Authors: Dhanani, Karim Colin Hassan
- Date: 2015
- Subjects: Uncatalogued
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/193487 , vital:45336
- Description: Heat shock protein 90 (Hsp90) and heat shock factors (HSFs) are known to be involved in the epigenetic regulation of several fundamental oncogenic genes. Fibronectin (FN) is an extracellular matrix (ECM) glycoprotein which plays key roles in cell adhesion and migration. Hsp90 binds directly to FN and Hsp90 inhibition has been shown to regulate FN protein levels and matrix formation. Where inhibition of Hsp90 with a C-terminal inhibitor (novobiocin) induced the loss of FN matrix, treatment with an N-terminal inhibitor (geldanamycin) increased FN matrix levels. GA treatment induced a strong dose and time dependent increase in FN1 promoter activity and increased total FN mRNA respectively. By contrast, NOV showed no increase in the promoter activity and no change in the expression of FN mRNA. As GA is known to induce the stress response, we investigated the relationship between the cell stress machinery and the transcriptional regulation of FN. Three putative heat shock elements (HSEs) were identified in the FN1 promoter. The loss of two of the three identified putative HSEs resulted in a loss in the basal transcriptional activity of the FN1 promoter in our reporter model. This was in addition to the loss of the induction of transcriptional activity with GA treatment observed with the full-length promoter. Binding of HSF1 to one of the putative HSEs, which was identified as potentially functional from the truncation analysis, was confirmed using ChIP. The occupancy of this HSE by HSF1 was shown to increase with GA treatment. These data support the hypothesis that FN1 is a functional HSF1 target gene. The 5' promoter regions of seven additional ECM protein encoding genes were analysed and mRNA levels were detected by quantitative RT-PCR upon treatment with GA. Collagen 4 _2 and laminin _3 mRNA were found to increase in the presence of GA, whereas collagen 4 _3 and osteopontin showed no change. Similarly to FN1, these data indicate that a subset of ECM genes may be under the regulation of the HSF1 mediated heat-shock response. This may have implications for our understanding of ECM dynamics in cancer, where the clinical application of Hsp90 inhibitors is intended. Additionally, our data provide a poten- tial underpinning for the role of the HSF1 mediated heat-shock response in several fibrotic and metabolic stress related pathologies. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2015
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Human harvesting impacts on managed areas: ecological effects of socially-compatible shellfish reserves
- Aswani, Shankar, Flores, Carola F, Broitman, Bernardo R
- Authors: Aswani, Shankar , Flores, Carola F , Broitman, Bernardo R
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/124664 , vital:35644 , https://doi.10.1007/s11160-014-9376-4
- Description: We examined how human harvesting impacts on managed areas affect the abundance and size distribution of the edible mangrove shellfish Anadara granosa and Polymesoda spp. in the Roviana Lagoon, Solomon Islands. We tested two hypotheses: (1) in areas permanently and temporally closed to human exploitation, abundance and size distribution of these shellfish species is significantly greater than in sites open to exploitation and (2) moderate human disturbance of shell beds, particularly of Polymesoda spp., increases their abundance. Firstly, we studied perceptions of environmental states and processes coupled to foraging and management interventions to assess sociocultural influences on harvesting practices and ascertain the types of management regime that people would consider in a context where poaching and interloping are common practices. Secondly, we compared shellfish abundance and shell size from areas that were permanently protected, temporally reserved for communal harvest, and permanently open for exploitation. Thirdly, drawing from women’s local knowledge, we measured the abundance of Polymesoda spp. in relation to mud compactness in quadrats across the three management regimes. Results showed that both species were significantly more abundant in permanent and temporally closed sites than in open sites. In the mud compactness study, however, while shell abundance was greater in moderately compacted quadrats, there was no statistical relationship between mud compactness and shell abundance within or across the three management regimes. Results suggest that even under the strong impacts of poaching, temporally closed areas have more clams than open areas and are as effective as areas that are permanently closed nominally. The results also suggest that human harvesting regimes can influence the effectiveness of local management decisions and thus are important when designing community-based conservation programs in the Solomon Islands and other Pacific Islands.
- Full Text:
- Authors: Aswani, Shankar , Flores, Carola F , Broitman, Bernardo R
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/124664 , vital:35644 , https://doi.10.1007/s11160-014-9376-4
- Description: We examined how human harvesting impacts on managed areas affect the abundance and size distribution of the edible mangrove shellfish Anadara granosa and Polymesoda spp. in the Roviana Lagoon, Solomon Islands. We tested two hypotheses: (1) in areas permanently and temporally closed to human exploitation, abundance and size distribution of these shellfish species is significantly greater than in sites open to exploitation and (2) moderate human disturbance of shell beds, particularly of Polymesoda spp., increases their abundance. Firstly, we studied perceptions of environmental states and processes coupled to foraging and management interventions to assess sociocultural influences on harvesting practices and ascertain the types of management regime that people would consider in a context where poaching and interloping are common practices. Secondly, we compared shellfish abundance and shell size from areas that were permanently protected, temporally reserved for communal harvest, and permanently open for exploitation. Thirdly, drawing from women’s local knowledge, we measured the abundance of Polymesoda spp. in relation to mud compactness in quadrats across the three management regimes. Results showed that both species were significantly more abundant in permanent and temporally closed sites than in open sites. In the mud compactness study, however, while shell abundance was greater in moderately compacted quadrats, there was no statistical relationship between mud compactness and shell abundance within or across the three management regimes. Results suggest that even under the strong impacts of poaching, temporally closed areas have more clams than open areas and are as effective as areas that are permanently closed nominally. The results also suggest that human harvesting regimes can influence the effectiveness of local management decisions and thus are important when designing community-based conservation programs in the Solomon Islands and other Pacific Islands.
- Full Text:
Human myiasis in rural South Africa is under-reported
- Kuria, Simon K, Villet, Martin H, Kingu, H J C, Dhaffala, A
- Authors: Kuria, Simon K , Villet, Martin H , Kingu, H J C , Dhaffala, A
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/442425 , vital:73983 , https://www.ajol.info/index.php/samj/article/view/114028
- Description: Background. Myiasis is the infestation of live tissue of humans and other vertebrates by larvae of flies. Worldwide, myiasis of humans is seldom reported, although the trend is gradually changing in some countries. Reports of human myiasis in Africa are few. Several cases of myiasis were recently seen at the Mthatha Hospital Complex, Mthatha, Eastern Cape Province, South Africa (SA).
- Full Text:
- Authors: Kuria, Simon K , Villet, Martin H , Kingu, H J C , Dhaffala, A
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/442425 , vital:73983 , https://www.ajol.info/index.php/samj/article/view/114028
- Description: Background. Myiasis is the infestation of live tissue of humans and other vertebrates by larvae of flies. Worldwide, myiasis of humans is seldom reported, although the trend is gradually changing in some countries. Reports of human myiasis in Africa are few. Several cases of myiasis were recently seen at the Mthatha Hospital Complex, Mthatha, Eastern Cape Province, South Africa (SA).
- Full Text:
Human, vector and parasite Hsp90 proteins: a comparative bioinformatics analysis
- Faya, Ngonidzashe, Penkler, David L, Tastan Bishop, Özlem
- Authors: Faya, Ngonidzashe , Penkler, David L , Tastan Bishop, Özlem
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/148038 , vital:38704 , DOI: 10.1016/j.fob.2015.11.003
- Description: The treatment of protozoan parasitic diseases is challenging, and thus identification and analysis of new drug targets is important. Parasites survive within host organisms, and some need intermediate hosts to complete their life cycle. Changing host environment puts stress on parasites, and often adaptation is accompanied by the expression of large amounts of heat shock proteins (Hsps). Among Hsps, Hsp90 proteins play an important role in stress environments. Yet, there has been little computational research on Hsp90 proteins to analyze them comparatively as potential parasitic drug targets. Here, an attempt was made to gain detailed insights into the differences between host, vector and parasitic Hsp90 proteins by large-scale bioinformatics analysis.
- Full Text:
- Authors: Faya, Ngonidzashe , Penkler, David L , Tastan Bishop, Özlem
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/148038 , vital:38704 , DOI: 10.1016/j.fob.2015.11.003
- Description: The treatment of protozoan parasitic diseases is challenging, and thus identification and analysis of new drug targets is important. Parasites survive within host organisms, and some need intermediate hosts to complete their life cycle. Changing host environment puts stress on parasites, and often adaptation is accompanied by the expression of large amounts of heat shock proteins (Hsps). Among Hsps, Hsp90 proteins play an important role in stress environments. Yet, there has been little computational research on Hsp90 proteins to analyze them comparatively as potential parasitic drug targets. Here, an attempt was made to gain detailed insights into the differences between host, vector and parasitic Hsp90 proteins by large-scale bioinformatics analysis.
- Full Text: