Optimisation of an electrochemical impedance spectroscopy aptasensor by exploiting quartz crystal microbalance with dissipation signals
- Formisanoa, Nello, Jolly, Pawan, Bhalla, Nikhil, Cromhout, Mary, Flanagan, Shane P, Fogel, Ronen, Limson, Janice L, Estrela, Pedro
- Authors: Formisanoa, Nello , Jolly, Pawan , Bhalla, Nikhil , Cromhout, Mary , Flanagan, Shane P , Fogel, Ronen , Limson, Janice L , Estrela, Pedro
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/431699 , vital:72797 , xlink:href="https://doi.org/10.1016/j.snb.2015.05.049"
- Description: The response of an Electrochemical Impedance Spectroscopy (EIS) sensor using DNA aptamers is affected by many factors, such as DNA density, charge and conformational changes upon DNA-target binding, and buffer conditions. We report here for the first time on the optimisation of an EIS aptamer-based sensor by using Quartz Crystal Microbalance with Dissipation mode (QCM-D). As a case study, we employed a DNA aptamer against Prostate-Specific Antigen (PSA). PSA detection was achieved by functionalising the gold sensor surface via thiol chemistry with different ratios of thiolated-DNA aptamer and 6-mercapto-1-hexanol (MCH) used as spacer molecules. PSA binding efficiency can be monitored by measuring QCM-D signals which not only provide information about the mass of PSA bound on the sensor surface, but also crucial information about the aptamer conformation and layer hydration.
- Full Text:
- Authors: Formisanoa, Nello , Jolly, Pawan , Bhalla, Nikhil , Cromhout, Mary , Flanagan, Shane P , Fogel, Ronen , Limson, Janice L , Estrela, Pedro
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/431699 , vital:72797 , xlink:href="https://doi.org/10.1016/j.snb.2015.05.049"
- Description: The response of an Electrochemical Impedance Spectroscopy (EIS) sensor using DNA aptamers is affected by many factors, such as DNA density, charge and conformational changes upon DNA-target binding, and buffer conditions. We report here for the first time on the optimisation of an EIS aptamer-based sensor by using Quartz Crystal Microbalance with Dissipation mode (QCM-D). As a case study, we employed a DNA aptamer against Prostate-Specific Antigen (PSA). PSA detection was achieved by functionalising the gold sensor surface via thiol chemistry with different ratios of thiolated-DNA aptamer and 6-mercapto-1-hexanol (MCH) used as spacer molecules. PSA binding efficiency can be monitored by measuring QCM-D signals which not only provide information about the mass of PSA bound on the sensor surface, but also crucial information about the aptamer conformation and layer hydration.
- Full Text:
Optimisation of the emulsion liquid membrane composition and demulsification for rhodium extraction
- Moyo, Francis, Tandlich, Roman
- Authors: Moyo, Francis , Tandlich, Roman
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/76743 , vital:30626 , https://doi.org/10.1515/lwr-2015-0002
- Description: This study was aimed at designing an optimised emulsion liquid membrane (ELM) for the extraction of rhodium from precious metal refinery wastewaters. The demulsification process and the structure of the optimised ELM are reported on. Two optimised ELMs were prepared. The first one contained a 30 % solution of toluene in kerosene as diluent with the following concentrations of the ELM components: 30.000 g/L (w/v) polyisobutylene, 10.870 g/L (m/v) of trioctyl amine and 51.001 g/L (m/v) of SPAN 80. The second ELM contained the same diluent, but the concentrations of the other ELM components in it were as follows: 20.000 g/l of polyisobutylene, 10.268 g/l trioctyl amine and 50.024 g/l of SPAN 80. The stripping phase was the same in both optimised ELMs, namely a 2 M solution of HNO3. The stripping phase and the diluent solution were mixed together in ratios of 1:1 and 2:1, respectively. Two methods were used to characterise the microdroplet diameters, i.e. optical microscopy and the Zeta-sizer. For the t-test, the p-value of 0.3018 at 5 % level of significance showed that there was statistically no significant difference in the mean micro-droplet size for 1:2 ELMs containing 20 g/l and 30 g/l of polyisobutylene after 40 minutes of emulsification. The best demulsification results were obtained using the chemical demulsification with polyethylene glycol with molecular weight of 400 g/mol (PEG 400) at 50 ± 1 °C for 24 hours. However, significant carryover of toluene, trioctyl amine and polyethylene glycol into the aqueous phase was observed.
- Full Text:
- Authors: Moyo, Francis , Tandlich, Roman
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/76743 , vital:30626 , https://doi.org/10.1515/lwr-2015-0002
- Description: This study was aimed at designing an optimised emulsion liquid membrane (ELM) for the extraction of rhodium from precious metal refinery wastewaters. The demulsification process and the structure of the optimised ELM are reported on. Two optimised ELMs were prepared. The first one contained a 30 % solution of toluene in kerosene as diluent with the following concentrations of the ELM components: 30.000 g/L (w/v) polyisobutylene, 10.870 g/L (m/v) of trioctyl amine and 51.001 g/L (m/v) of SPAN 80. The second ELM contained the same diluent, but the concentrations of the other ELM components in it were as follows: 20.000 g/l of polyisobutylene, 10.268 g/l trioctyl amine and 50.024 g/l of SPAN 80. The stripping phase was the same in both optimised ELMs, namely a 2 M solution of HNO3. The stripping phase and the diluent solution were mixed together in ratios of 1:1 and 2:1, respectively. Two methods were used to characterise the microdroplet diameters, i.e. optical microscopy and the Zeta-sizer. For the t-test, the p-value of 0.3018 at 5 % level of significance showed that there was statistically no significant difference in the mean micro-droplet size for 1:2 ELMs containing 20 g/l and 30 g/l of polyisobutylene after 40 minutes of emulsification. The best demulsification results were obtained using the chemical demulsification with polyethylene glycol with molecular weight of 400 g/mol (PEG 400) at 50 ± 1 °C for 24 hours. However, significant carryover of toluene, trioctyl amine and polyethylene glycol into the aqueous phase was observed.
- Full Text:
Organosilicon compounds as fluorescent chemosensors for fluoride anion recognition
- Gai, Lizhi, Mack, John, Lu, Hua, Nyokong, Tebello, Li, Zhifang, Kobayashi, Nagao, Shen, Zhen
- Authors: Gai, Lizhi , Mack, John , Lu, Hua , Nyokong, Tebello , Li, Zhifang , Kobayashi, Nagao , Shen, Zhen
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/241424 , vital:50938 , xlink:href="https://doi.org/10.1016/j.ccr.2014.10.009"
- Description: Recent developments in organosilicon-based chemosensors for F− recognition are reviewed. The design strategies for improving the photophysical properties of organosilicon-based chemosensors are elaborated, with an emphasis placed on their utility for biological applications. The photophysical properties and electronic structures are analyzed in depth with reference made to the results of molecular modeling calculation and possible future research directions are assessed.
- Full Text:
- Authors: Gai, Lizhi , Mack, John , Lu, Hua , Nyokong, Tebello , Li, Zhifang , Kobayashi, Nagao , Shen, Zhen
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/241424 , vital:50938 , xlink:href="https://doi.org/10.1016/j.ccr.2014.10.009"
- Description: Recent developments in organosilicon-based chemosensors for F− recognition are reviewed. The design strategies for improving the photophysical properties of organosilicon-based chemosensors are elaborated, with an emphasis placed on their utility for biological applications. The photophysical properties and electronic structures are analyzed in depth with reference made to the results of molecular modeling calculation and possible future research directions are assessed.
- Full Text:
Overexpression, Purification and Characterisation of the Plasmodium falciparum Hsp70-z (PfHsp70-z) Protein
- Zininga, Tawanda, Achilonu, Ikechukwu, Hoppe, Heinrich C, Prinsloo, Earl, Dirr, Heinrich W, Shonhai, Addmore
- Authors: Zininga, Tawanda , Achilonu, Ikechukwu , Hoppe, Heinrich C , Prinsloo, Earl , Dirr, Heinrich W , Shonhai, Addmore
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/431719 , vital:72799 , xlink:href="https://doi.org/10.1371/journal.pone.0129445"
- Description: Six Hsp70-like genes are represented on the genome of Plasmodium falciparum. Of these two occur in the cytosol: P. falciparum Hsp70-z (PfHsp70-z) and PfHsp70-1. PfHsp70-1 is a well characterised canonical Hsp70 that facilitates protein quality control and is crucial for the development of malaria parasites. There is very little known about PfHsp70-z. However, PfHsp70-z is known to be essential and is implicated in suppressing aggregation of asparagine-rich proteins of P. falciparum. In addition, its expression at the clinical stage of malaria correlates with disease prognosis. Based on structural evidence PfHsp70-z belongs to the Hsp110 family of proteins. Since Hsp110 proteins have been described as nucleotide exchange factors (NEFs) of their canonical Hsp70 counterparts, it has been speculated that PfHsp70-z may serve as a NEF of PfHsp70-1. In the current study, P. falciparum cells cultured in vitro were subjected to heat stress, triggering the enhanced expression of PfHsp70-z. Biochemical assays conducted using recombinant PfHsp70-z protein demonstrated that the protein is heat stable and possesses ATPase activity. Furthermore, we observed that PfHsp70-z is capable of self-association. The structural-functional features of PfHsp70-z provide further evidence for its role as a chaperone and possible nucleotide exchange factor of PfHsp70-1.
- Full Text:
- Authors: Zininga, Tawanda , Achilonu, Ikechukwu , Hoppe, Heinrich C , Prinsloo, Earl , Dirr, Heinrich W , Shonhai, Addmore
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/431719 , vital:72799 , xlink:href="https://doi.org/10.1371/journal.pone.0129445"
- Description: Six Hsp70-like genes are represented on the genome of Plasmodium falciparum. Of these two occur in the cytosol: P. falciparum Hsp70-z (PfHsp70-z) and PfHsp70-1. PfHsp70-1 is a well characterised canonical Hsp70 that facilitates protein quality control and is crucial for the development of malaria parasites. There is very little known about PfHsp70-z. However, PfHsp70-z is known to be essential and is implicated in suppressing aggregation of asparagine-rich proteins of P. falciparum. In addition, its expression at the clinical stage of malaria correlates with disease prognosis. Based on structural evidence PfHsp70-z belongs to the Hsp110 family of proteins. Since Hsp110 proteins have been described as nucleotide exchange factors (NEFs) of their canonical Hsp70 counterparts, it has been speculated that PfHsp70-z may serve as a NEF of PfHsp70-1. In the current study, P. falciparum cells cultured in vitro were subjected to heat stress, triggering the enhanced expression of PfHsp70-z. Biochemical assays conducted using recombinant PfHsp70-z protein demonstrated that the protein is heat stable and possesses ATPase activity. Furthermore, we observed that PfHsp70-z is capable of self-association. The structural-functional features of PfHsp70-z provide further evidence for its role as a chaperone and possible nucleotide exchange factor of PfHsp70-1.
- Full Text:
Oxford dictionary of journalism:
- Authors: Amner, Roderick J
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/142656 , vital:38099 , DOI: 10.1080/23743670.2015.1041305
- Description: Tony Harcup is a self-confessed ‘hackademic’, a term which he defines in his Oxford dictionary of journalism as ‘a journalist who goes on to work in journalism education where they combine the roles of journalism (hack) and academic’ (p. 121). Harcup explains that hackademics perform this balancing act in the ‘hackademy’, a curious space within academe where journalism training intersects with Journalism Studies (a field of study which is nowadays often granted ‘upper case’ status), ‘sometimes with mutual respect and insight, sometimes with mutual suspicion and hostility’ (ibid.). And this tension is never far from the surface in many of the 1 300 definitions in this useful and absorbing dictionary.
- Full Text:
- Authors: Amner, Roderick J
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/142656 , vital:38099 , DOI: 10.1080/23743670.2015.1041305
- Description: Tony Harcup is a self-confessed ‘hackademic’, a term which he defines in his Oxford dictionary of journalism as ‘a journalist who goes on to work in journalism education where they combine the roles of journalism (hack) and academic’ (p. 121). Harcup explains that hackademics perform this balancing act in the ‘hackademy’, a curious space within academe where journalism training intersects with Journalism Studies (a field of study which is nowadays often granted ‘upper case’ status), ‘sometimes with mutual respect and insight, sometimes with mutual suspicion and hostility’ (ibid.). And this tension is never far from the surface in many of the 1 300 definitions in this useful and absorbing dictionary.
- Full Text:
Parental involvement in school governance: a case study of a secondary school in Okahandja, Namibia
- Authors: Shikwambi, Victoria
- Date: 2015
- Subjects: Parent-teacher relationships -- Namibia -- Okahandja , Education -- Parent participation -- Namibia -- Okahandja , School management and organization -- Namibia -- Okahandja , School boards -- Namibia -- Okahandja
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:2047 , http://hdl.handle.net/10962/d1017358
- Description: Since Namibia gained independence parents have increasingly been seen as equal partners in the education process. The Education Act 16 of 2001 provides for the democratic participation in schools by parents, learners and other education stakeholders through the introduction of regional Education Forums and School Boards in schools Parental involvement in school governance has been widely researched and has become a ‘hot topic’ worldwide, including in Namibia. This study investigated and described parental involvement through the School Board in school governance in a secondary school in Otjozondjupa region, Namibia. The study was conducted within a qualitative, interpretive paradigm. The study employed three data collection tools namely, semi-structured interviews, document analysis and observation. The School Board was made up of parents of different classes and backgrounds, i.e. employed, unemployed, professionals and business persons and with different levels of educational background. These members create a network that represents the voices of parents from different social groups, with different levels of social capital. The different levels of social capital shape the nature of the contributions and interactions on the Board. The study found that in spite of the frequency of interaction between the School Board, parents and the community the School Board is still in a dilemma as it is unable to connect with its prominent source of potential support, such as the business community, due to an outdated view that the school is well-off based on its historic status of privilege. With respect to communications with parents the focus tends to be on the negatives of learners’ behaviour or performance and the task of the parents in this regard. Broadening the agenda of the collaboration to include positive aspects of the child would add to the motivation of parents and open possibilities for new forms of collaboration. The school lacks a well-coordinated system for utilizing the available resources as well as community expertise for the benefit of the school. On the strength of the findings, one of the recommendations is for a more structured program and strategy for the Boards various interactions with the community and parents.
- Full Text:
- Authors: Shikwambi, Victoria
- Date: 2015
- Subjects: Parent-teacher relationships -- Namibia -- Okahandja , Education -- Parent participation -- Namibia -- Okahandja , School management and organization -- Namibia -- Okahandja , School boards -- Namibia -- Okahandja
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:2047 , http://hdl.handle.net/10962/d1017358
- Description: Since Namibia gained independence parents have increasingly been seen as equal partners in the education process. The Education Act 16 of 2001 provides for the democratic participation in schools by parents, learners and other education stakeholders through the introduction of regional Education Forums and School Boards in schools Parental involvement in school governance has been widely researched and has become a ‘hot topic’ worldwide, including in Namibia. This study investigated and described parental involvement through the School Board in school governance in a secondary school in Otjozondjupa region, Namibia. The study was conducted within a qualitative, interpretive paradigm. The study employed three data collection tools namely, semi-structured interviews, document analysis and observation. The School Board was made up of parents of different classes and backgrounds, i.e. employed, unemployed, professionals and business persons and with different levels of educational background. These members create a network that represents the voices of parents from different social groups, with different levels of social capital. The different levels of social capital shape the nature of the contributions and interactions on the Board. The study found that in spite of the frequency of interaction between the School Board, parents and the community the School Board is still in a dilemma as it is unable to connect with its prominent source of potential support, such as the business community, due to an outdated view that the school is well-off based on its historic status of privilege. With respect to communications with parents the focus tends to be on the negatives of learners’ behaviour or performance and the task of the parents in this regard. Broadening the agenda of the collaboration to include positive aspects of the child would add to the motivation of parents and open possibilities for new forms of collaboration. The school lacks a well-coordinated system for utilizing the available resources as well as community expertise for the benefit of the school. On the strength of the findings, one of the recommendations is for a more structured program and strategy for the Boards various interactions with the community and parents.
- Full Text:
Pathologies of vision : representations of deviant women and the cyborg body
- Authors: Rheeder, Elle-Sandrah
- Date: 2015
- Subjects: Haraway, Donna Jeanne -- Criticism and interpretation , Deviant behavior in art , Cyborgs in art , Cyborgs in motion pictures , Women in art , Socialist feminism , Hysteria in literature , Hysteria -- History , Science fiction -- History and criticism , Visual communication in art
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2514 , http://hdl.handle.net/10962/d1020319
- Description: This thesis investigates the figure of the cyborg as conceptualised by Donna Haraway in The Cyborg Manifesto (1991). The figure of the cyborg, as a transgressive figure in the late twentieth century within socialist feminist discourse, is problematized with regard to its efficacy as a creature that challenges the constructed nature of gender and contests the boundary between human and machine through its ambiguous nature. Haraway’s notions of the cyborg, which she bases partly on cyborg characters from Science Fiction literature, deny the ocularcentric traditions that have structured gender and the body. Similarly, Haraway does not engage adequately with the figure of the cyborg with regard to situating it historically. This thesis unpacks both the visual and the historical aspects that have structured the cyborg body. By engaging with these concepts, the cyborg emerges as a figure that is identified through visual signifiers of female deviance and pathology. By reading female deviance and pathology on the body of the nineteenth-century hysteric, similarities can be drawn between the hysteric and the cyborg. Through a reading of Alien (1979); Blade Runner (1982); and Star Trek: First Contact (1996) key cyborg texts of the late twentieth century, the figure of the cyborg, and its relation to the deviant pathologised female can be understood when read against the body of the hysteric and how it was visually coded and communicated
- Full Text:
- Authors: Rheeder, Elle-Sandrah
- Date: 2015
- Subjects: Haraway, Donna Jeanne -- Criticism and interpretation , Deviant behavior in art , Cyborgs in art , Cyborgs in motion pictures , Women in art , Socialist feminism , Hysteria in literature , Hysteria -- History , Science fiction -- History and criticism , Visual communication in art
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:2514 , http://hdl.handle.net/10962/d1020319
- Description: This thesis investigates the figure of the cyborg as conceptualised by Donna Haraway in The Cyborg Manifesto (1991). The figure of the cyborg, as a transgressive figure in the late twentieth century within socialist feminist discourse, is problematized with regard to its efficacy as a creature that challenges the constructed nature of gender and contests the boundary between human and machine through its ambiguous nature. Haraway’s notions of the cyborg, which she bases partly on cyborg characters from Science Fiction literature, deny the ocularcentric traditions that have structured gender and the body. Similarly, Haraway does not engage adequately with the figure of the cyborg with regard to situating it historically. This thesis unpacks both the visual and the historical aspects that have structured the cyborg body. By engaging with these concepts, the cyborg emerges as a figure that is identified through visual signifiers of female deviance and pathology. By reading female deviance and pathology on the body of the nineteenth-century hysteric, similarities can be drawn between the hysteric and the cyborg. Through a reading of Alien (1979); Blade Runner (1982); and Star Trek: First Contact (1996) key cyborg texts of the late twentieth century, the figure of the cyborg, and its relation to the deviant pathologised female can be understood when read against the body of the hysteric and how it was visually coded and communicated
- Full Text:
Patriarchy, women, land and livelihoods on A1 farms in Zimbabwe
- Chiweshe, Manase K, Chakona, Loveness, Helliker, Kirk D
- Authors: Chiweshe, Manase K , Chakona, Loveness , Helliker, Kirk D
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/71204 , vital:29812 , https://doi.org/10.1177/0021909614541083
- Description: In this article, we focus on patriarchy, women, land and livelihoods on A1 farms in Zimbabwe which arose from the fast track land reform programme. There is now significant literature on A1 farms in Zimbabwe. These studies include a number of ethnographic and comparative studies but this literature does not give sustained attention to patriarchy and women. In addition, though, a small number of works have appeared based upon a more focused gender analysis. We draw upon this more focused literature and offer fresh fieldwork evidence based on recent studies undertaken by two of the authors, in Goromonzi and Mazowe districts. At times, radical socio-spatial reorganisation such as fast track may destabilise systems of patriarchy. In the case of fast track, there has been a reconfiguration of relations between men and women yet this is uneven and contradictory and remains within the confines of patriarchal structures, practices and discourses. At the same time, women have manoeuvred and negotiated at local levels to enhance their lives and livelihoods.
- Full Text: false
- Authors: Chiweshe, Manase K , Chakona, Loveness , Helliker, Kirk D
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/71204 , vital:29812 , https://doi.org/10.1177/0021909614541083
- Description: In this article, we focus on patriarchy, women, land and livelihoods on A1 farms in Zimbabwe which arose from the fast track land reform programme. There is now significant literature on A1 farms in Zimbabwe. These studies include a number of ethnographic and comparative studies but this literature does not give sustained attention to patriarchy and women. In addition, though, a small number of works have appeared based upon a more focused gender analysis. We draw upon this more focused literature and offer fresh fieldwork evidence based on recent studies undertaken by two of the authors, in Goromonzi and Mazowe districts. At times, radical socio-spatial reorganisation such as fast track may destabilise systems of patriarchy. In the case of fast track, there has been a reconfiguration of relations between men and women yet this is uneven and contradictory and remains within the confines of patriarchal structures, practices and discourses. At the same time, women have manoeuvred and negotiated at local levels to enhance their lives and livelihoods.
- Full Text: false
Pecha Kucha 1: Critical Studies in Sexualities and Reproduction
- Authors: Macleod, Catriona I
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/143915 , vital:38294 , https://ischp.files.wordpress.com/2015/08/ischp_2015_abstract_booklet.pdf
- Description: Despite enabling legislation and policies in the areas of sexualities and reproduction in South Africa, multiple challenges persist, including: forced sexual debut, sexual coercion and violence; HIV infection; hate crimes against lesbian women and gay men; unwanted and unsupportable pregnancies. While it is acknowledged that interventions (e.g., sexuality education programmes, the promotion of antenatal care use and the promotion of non-discrimination) have the potential to improve men’s and women’s sexual and reproductive lives. There are also multiple ways in which such programmes and the surrounding public discourses concerning sexuality and reproduction can serve in often unintended and unwitting ways to perpetuate oppressive heteronormative, gendered, racialised and class-based power relations. The Critical Studies in Sexualities and Reproduction research programme focuses on how particular discourses, narratives, and practices promote inclusion or exclusion, belonging or marginalisation, equity or inequity, justice or injustice, access to, or denial of, sexual and reproductive rights.
- Full Text:
- Authors: Macleod, Catriona I
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/143915 , vital:38294 , https://ischp.files.wordpress.com/2015/08/ischp_2015_abstract_booklet.pdf
- Description: Despite enabling legislation and policies in the areas of sexualities and reproduction in South Africa, multiple challenges persist, including: forced sexual debut, sexual coercion and violence; HIV infection; hate crimes against lesbian women and gay men; unwanted and unsupportable pregnancies. While it is acknowledged that interventions (e.g., sexuality education programmes, the promotion of antenatal care use and the promotion of non-discrimination) have the potential to improve men’s and women’s sexual and reproductive lives. There are also multiple ways in which such programmes and the surrounding public discourses concerning sexuality and reproduction can serve in often unintended and unwitting ways to perpetuate oppressive heteronormative, gendered, racialised and class-based power relations. The Critical Studies in Sexualities and Reproduction research programme focuses on how particular discourses, narratives, and practices promote inclusion or exclusion, belonging or marginalisation, equity or inequity, justice or injustice, access to, or denial of, sexual and reproductive rights.
- Full Text:
Pecha Kucha 2: Race and gender politics in cross-dressing, dressing-up, and drag
- Authors: Marx, Jacqueline
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/143926 , vital:38295 , https://ischp.files.wordpress.com/2015/08/ischp_2015_abstract_booklet.pdf
- Description: Sexuality is often less discernible than other markers of identity such as race and gender, which means that its perceptibility is often contingent on strategies that make it visible. Visibility is an especially important dimension in the shaping of lesbian and gay identities because people are generally assumed to be heterosexual. Making gay and lesbian identities visible is also an important political issue for people who have, historically, been threatened by the violence of public erasure. It is this aspect of gay and lesbian subjectivity – the politics of visibility – that is the topic of this presentation. We focus on gender and race politics in dressing-up, cross-dressing and drag performances - practices which make gay and lesbian sexualities visible, and consider the role that race and gender play in diminishing and exacerbating visibility.
- Full Text:
- Authors: Marx, Jacqueline
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/143926 , vital:38295 , https://ischp.files.wordpress.com/2015/08/ischp_2015_abstract_booklet.pdf
- Description: Sexuality is often less discernible than other markers of identity such as race and gender, which means that its perceptibility is often contingent on strategies that make it visible. Visibility is an especially important dimension in the shaping of lesbian and gay identities because people are generally assumed to be heterosexual. Making gay and lesbian identities visible is also an important political issue for people who have, historically, been threatened by the violence of public erasure. It is this aspect of gay and lesbian subjectivity – the politics of visibility – that is the topic of this presentation. We focus on gender and race politics in dressing-up, cross-dressing and drag performances - practices which make gay and lesbian sexualities visible, and consider the role that race and gender play in diminishing and exacerbating visibility.
- Full Text:
Pecha Kucha 3: Memories, photographs, and archives: On historicising ‘puberty’ in the present
- Authors: Pinto, Pedro
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/143937 , vital:38296 , https://ischp.files.wordpress.com/2015/08/ischp_2015_abstract_booklet.pdf
- Description: In 1997, Valerie Walkerdine incited researchers to use memory and fantasy as devices to explore the gendered meanings of being a child and becoming adult. Since we all once were children, we are in the ambiguous – and therefore productive – position of being both informants and ethnographers of childhood experiences. Whilst drawing on a historical investigation on puberty science, this presentation is a hybrid experiment: it combines my memories of growing up in Portugal in the early 1980s and photographs of children taken in clinical interventions from the late nineteenth century to the first decades of the twentieth. In articulating these materials and their different temporalities, my purpose is to explore the techniques and the power relations in which pubertal development came to being thought of, and disciplined, in contemporary societies. In doing so, I invite the possibility of historicising experience as a strategy to critically analyse our medicalised present.
- Full Text:
- Authors: Pinto, Pedro
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/143937 , vital:38296 , https://ischp.files.wordpress.com/2015/08/ischp_2015_abstract_booklet.pdf
- Description: In 1997, Valerie Walkerdine incited researchers to use memory and fantasy as devices to explore the gendered meanings of being a child and becoming adult. Since we all once were children, we are in the ambiguous – and therefore productive – position of being both informants and ethnographers of childhood experiences. Whilst drawing on a historical investigation on puberty science, this presentation is a hybrid experiment: it combines my memories of growing up in Portugal in the early 1980s and photographs of children taken in clinical interventions from the late nineteenth century to the first decades of the twentieth. In articulating these materials and their different temporalities, my purpose is to explore the techniques and the power relations in which pubertal development came to being thought of, and disciplined, in contemporary societies. In doing so, I invite the possibility of historicising experience as a strategy to critically analyse our medicalised present.
- Full Text:
Perceived breach and violation of the psychological contract in a collectivistic culture
- Van der Merwe, Sophie Wilhelmine
- Authors: Van der Merwe, Sophie Wilhelmine
- Date: 2015
- Subjects: Collectivism , Employees -- Attitudes , Contracts -- Psychological aspects , Industrial relations , Breach of contract , Personnel management -- Psychological aspects , Organizational behavior , Psychology, Industrial
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:855 , http://hdl.handle.net/10962/d1017523
- Description: The significance of relationships on economic actions and employee behaviour makes it critical for employers to understand the dynamics of employment through mutual obligations (Rousseau, 1990; Guest, 2004b). The psychological contract affords a broad platform to study the employment relationship (Thomas et al., 2010), and is an important tool for organisational success (McDermott et al., 2013). The literature review contained in this study indicates the differences in contracting environments due to the prevailing cultural orientation. However, there is a dearth of research in collectivistic culture, to which this study will add. The literature also makes a distinction between perceptions of breach and violation. While perceptions of breach of the psychological contract is the perception that the employer has not met all obligations and promises, violation is the emotional and affective state following breach (Morrison & Robinson, 1997) and results in negative or deviant behaviours (Chiu & Peng, 2008). Both breach and violation perceptions negatively affect employee behaviours and attitudes in the workplace (Aggarwal & Bhargava, 2014). This research assumed a constructivist paradigm and builds understanding of the outcomes of breach and violation of the psychological contract on employees’ working life in a collectivistic environment. Primary data collection was by in-depth semi-structured, one on one interviews with five employees of a state-subsidised organisation in East London, making use of convenience sampling. Follow up interviews were conducted, resulting in 7ₑ/₄ hours of interviewing time. Cultural orientation was ascertained through the use of a questionnaire. The findings of this study confirmed that the type of psychological contract entered into influences the outcomes of perceptions of breach and violation. Both dimensions of collectivism, namely institutional and in-group, were practised in this environment, which also impacted on both the individual’s experience and outcomes for the organisation. The latter was influenced by commitment to organisational goals and supervisory or collegial relationships. Most notable of the results is the effect of expectations of transitional justice on experience of the psychological contract. Practical implications and recommendations for future research are made. This research is presented in three sections; firstly the research is presented in the format of an academic paper and includes a concise summary of literature and research method. The second section is an expanded literature review of the psychological contract and its influencing factors, as well as the outcomes of breach and violation. The last section describes and justifies in detail the design of the research and the research procedure followed. , Alternate name: Van der Merwe, Somine
- Full Text:
- Authors: Van der Merwe, Sophie Wilhelmine
- Date: 2015
- Subjects: Collectivism , Employees -- Attitudes , Contracts -- Psychological aspects , Industrial relations , Breach of contract , Personnel management -- Psychological aspects , Organizational behavior , Psychology, Industrial
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:855 , http://hdl.handle.net/10962/d1017523
- Description: The significance of relationships on economic actions and employee behaviour makes it critical for employers to understand the dynamics of employment through mutual obligations (Rousseau, 1990; Guest, 2004b). The psychological contract affords a broad platform to study the employment relationship (Thomas et al., 2010), and is an important tool for organisational success (McDermott et al., 2013). The literature review contained in this study indicates the differences in contracting environments due to the prevailing cultural orientation. However, there is a dearth of research in collectivistic culture, to which this study will add. The literature also makes a distinction between perceptions of breach and violation. While perceptions of breach of the psychological contract is the perception that the employer has not met all obligations and promises, violation is the emotional and affective state following breach (Morrison & Robinson, 1997) and results in negative or deviant behaviours (Chiu & Peng, 2008). Both breach and violation perceptions negatively affect employee behaviours and attitudes in the workplace (Aggarwal & Bhargava, 2014). This research assumed a constructivist paradigm and builds understanding of the outcomes of breach and violation of the psychological contract on employees’ working life in a collectivistic environment. Primary data collection was by in-depth semi-structured, one on one interviews with five employees of a state-subsidised organisation in East London, making use of convenience sampling. Follow up interviews were conducted, resulting in 7ₑ/₄ hours of interviewing time. Cultural orientation was ascertained through the use of a questionnaire. The findings of this study confirmed that the type of psychological contract entered into influences the outcomes of perceptions of breach and violation. Both dimensions of collectivism, namely institutional and in-group, were practised in this environment, which also impacted on both the individual’s experience and outcomes for the organisation. The latter was influenced by commitment to organisational goals and supervisory or collegial relationships. Most notable of the results is the effect of expectations of transitional justice on experience of the psychological contract. Practical implications and recommendations for future research are made. This research is presented in three sections; firstly the research is presented in the format of an academic paper and includes a concise summary of literature and research method. The second section is an expanded literature review of the psychological contract and its influencing factors, as well as the outcomes of breach and violation. The last section describes and justifies in detail the design of the research and the research procedure followed. , Alternate name: Van der Merwe, Somine
- Full Text:
Perceived undersupply of local labour in the presence of unemployment: a case of selected Sundays River Valley citrus farms, 2013
- Authors: Chirara, Malon Tinotenda
- Date: 2015
- Subjects: Unemployment -- South Africa -- Sundays River (Eastern Cape) , Citrus fruit industry -- South Africa -- Sundays River (Eastern Cape) , Labor market -- South Africa -- Sundays River (Eastern Cape) , Work environment -- South Africa -- Sundays River (Eastern Cape) , Agricultural wages -- South Africa -- Sundays River (Eastern Cape) , Agricultural laborers -- South Africa -- Sundays River (Eastern Cape) , Seasonal labor -- South Africa -- Sundays River (Eastern Cape) , Migrant agricultural laborers -- South Africa -- Sundays River (Eastern Cape) , Personnel management -- South Africa -- Sundays River (Eastern Cape)
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:1125 , http://hdl.handle.net/10962/d1020367
- Description: While skilled labour shortages are common in many countries, including South Africa, mainly due to a skills mismatch, the undersupply of unskilled labour was less expected, especially in developing countries with high unemployment. The thesis utilises data on perceived worker undersupply on selected citrus farms in the Sundays River Valley (SRV), located in the Eastern Cape Province of South Africa, collected in 2013, to analyse why unemployed residents, surprisingly, do not fill up vacancies on farms. In contrast to other labour markets, farm employment is not restricted by educational levels and as workers reported, with little training the various job tasks and skills required are easy and quick to grasp. At a time the government is trying to find ways of reducing unemployment, and the SRV Municipality (2012:29) reported approximately 42% unemployment, the question arises as to why the relatively low educated residents do not take advantage of the employment opportunities on farms. According to local workers and unemployed residents, the farm job was unattractive largely because of a combination of two factors: perceived relatively low salaries partly caused by the availability of migrant seasonal workers accepting lower remuneration and poor non-wage working conditions. The survey also found that farmers preferred migrant workers because they were more productive compared to their local counterparts who were, reportedly, characterised by high absenteeism and laziness, caused mainly by a reliance on social grants and alcohol abuse. Other reasons given for the unattractiveness of the farm job included the seasonal nature of farm employment, which left workers with no source of income in the offseason, the redundancy associated with farm tasks, perceived poor treatment of workers and lack of information on UIF and Provident funds to farm workers. To address problems associated with the dislike of farm work, seasonality of on-farm employment and the reportedly relatively low income, farm managers, the local municipality and the Labour Department could possibly be involved in creating more communal agricultural projects and help provide local community members to venture into supporting alternative careers within the Hospitality, Ecotourism and Conservation Industries through training programmes. Farm managers may need to consider improving their working relationship with workers in communication and when assigning tasks. Farmers and the local municipality could also consider investing in training programmes for the unemployed residents to equip them with technical skills that can improve their chances of finding jobs.
- Full Text:
- Authors: Chirara, Malon Tinotenda
- Date: 2015
- Subjects: Unemployment -- South Africa -- Sundays River (Eastern Cape) , Citrus fruit industry -- South Africa -- Sundays River (Eastern Cape) , Labor market -- South Africa -- Sundays River (Eastern Cape) , Work environment -- South Africa -- Sundays River (Eastern Cape) , Agricultural wages -- South Africa -- Sundays River (Eastern Cape) , Agricultural laborers -- South Africa -- Sundays River (Eastern Cape) , Seasonal labor -- South Africa -- Sundays River (Eastern Cape) , Migrant agricultural laborers -- South Africa -- Sundays River (Eastern Cape) , Personnel management -- South Africa -- Sundays River (Eastern Cape)
- Language: English
- Type: Thesis , Masters , MCom
- Identifier: vital:1125 , http://hdl.handle.net/10962/d1020367
- Description: While skilled labour shortages are common in many countries, including South Africa, mainly due to a skills mismatch, the undersupply of unskilled labour was less expected, especially in developing countries with high unemployment. The thesis utilises data on perceived worker undersupply on selected citrus farms in the Sundays River Valley (SRV), located in the Eastern Cape Province of South Africa, collected in 2013, to analyse why unemployed residents, surprisingly, do not fill up vacancies on farms. In contrast to other labour markets, farm employment is not restricted by educational levels and as workers reported, with little training the various job tasks and skills required are easy and quick to grasp. At a time the government is trying to find ways of reducing unemployment, and the SRV Municipality (2012:29) reported approximately 42% unemployment, the question arises as to why the relatively low educated residents do not take advantage of the employment opportunities on farms. According to local workers and unemployed residents, the farm job was unattractive largely because of a combination of two factors: perceived relatively low salaries partly caused by the availability of migrant seasonal workers accepting lower remuneration and poor non-wage working conditions. The survey also found that farmers preferred migrant workers because they were more productive compared to their local counterparts who were, reportedly, characterised by high absenteeism and laziness, caused mainly by a reliance on social grants and alcohol abuse. Other reasons given for the unattractiveness of the farm job included the seasonal nature of farm employment, which left workers with no source of income in the offseason, the redundancy associated with farm tasks, perceived poor treatment of workers and lack of information on UIF and Provident funds to farm workers. To address problems associated with the dislike of farm work, seasonality of on-farm employment and the reportedly relatively low income, farm managers, the local municipality and the Labour Department could possibly be involved in creating more communal agricultural projects and help provide local community members to venture into supporting alternative careers within the Hospitality, Ecotourism and Conservation Industries through training programmes. Farm managers may need to consider improving their working relationship with workers in communication and when assigning tasks. Farmers and the local municipality could also consider investing in training programmes for the unemployed residents to equip them with technical skills that can improve their chances of finding jobs.
- Full Text:
Perceptions of being a learner: an investigation into how first year Journalism students at a South African university construct themselves as learners
- Authors: Lunga, Carolyne Mande
- Date: 2015
- Subjects: Journalism -- Study and teaching (Higher) -- South Africa , Students -- Attitudes , Students -- Self-rating of -- South Africa , Discourse analysis, Narrative , Active learning -- South Africa , Learning -- Evaluation , Learning, Psychology of , College freshmen -- South Africa
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1332 , http://hdl.handle.net/10962/d1020026
- Description: The aim of the research reported in this document was to explore the ways in which first year Journalism students at a South African University construct themselves as learners. The research adopted a case study approach of purposively selected first year journalism students. In exploring this area, focus group and individual in-depth interviewing were employed which illuminated important aspects of learner identity construction. In order to make sense of these self-constructions, the research was located in the larger debates on discourse as espoused by Michel Foucault who argues that discourse constructs subjectivities. The research demonstrated that there were various discourses at play which influenced how these learners spoke and behaved. The influence of these discourses on learners' experiences varied at different times of the year. For example, the awarding of the Duly Performed (DP) certificate for students who met the minimum attendance and work requirements of a particular course, the giving of tests, exercises and examinations were some of the technologies that 'forced' students into compliance. In terms of identity formation, the heterogeneous nature of 'being' a journalism 'student' revealed that the different discourses at play influenced learner behaviour and that their identities continued to change over the year. Doing additional subjects such as Sociology, Drama, Art History and others at the same time as Journalism and Media Studies also meant that the learners had to negotiate the differing role requirements.
- Full Text:
- Authors: Lunga, Carolyne Mande
- Date: 2015
- Subjects: Journalism -- Study and teaching (Higher) -- South Africa , Students -- Attitudes , Students -- Self-rating of -- South Africa , Discourse analysis, Narrative , Active learning -- South Africa , Learning -- Evaluation , Learning, Psychology of , College freshmen -- South Africa
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:1332 , http://hdl.handle.net/10962/d1020026
- Description: The aim of the research reported in this document was to explore the ways in which first year Journalism students at a South African University construct themselves as learners. The research adopted a case study approach of purposively selected first year journalism students. In exploring this area, focus group and individual in-depth interviewing were employed which illuminated important aspects of learner identity construction. In order to make sense of these self-constructions, the research was located in the larger debates on discourse as espoused by Michel Foucault who argues that discourse constructs subjectivities. The research demonstrated that there were various discourses at play which influenced how these learners spoke and behaved. The influence of these discourses on learners' experiences varied at different times of the year. For example, the awarding of the Duly Performed (DP) certificate for students who met the minimum attendance and work requirements of a particular course, the giving of tests, exercises and examinations were some of the technologies that 'forced' students into compliance. In terms of identity formation, the heterogeneous nature of 'being' a journalism 'student' revealed that the different discourses at play influenced learner behaviour and that their identities continued to change over the year. Doing additional subjects such as Sociology, Drama, Art History and others at the same time as Journalism and Media Studies also meant that the learners had to negotiate the differing role requirements.
- Full Text:
Perceptions of the natural environment from a rural African perspective: a case of Cylondropuntia fulgida var. fulgida in Gwanda district, Zimbabwe
- Dube, Nqobizitha, Snowball, Jeanette D, Fraser, Gavin C G
- Authors: Dube, Nqobizitha , Snowball, Jeanette D , Fraser, Gavin C G
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/68578 , vital:29289 , http://2015.essa.org.za/fullpaper/essa_2994.pdf
- Description: Publisher version , Community environmental perceptions are instrumental in environmental management programmes given that perspectives govern human-environment relations. Despite numerous studies on environmental perceptions, little is known about how the rural poor particularly in Africa conceptualize, live with, and respond to pressing environmental issues facing them. As such, this paper uses the case of an invasive alien plant (IAP) (Cylindropuntia fulgida var. fulgida (Cff)) in a rural community (Gwanda district, Zimbabwe) to unveil the conceptualisation of the natural environment from a rural African perspective. This paper discloses the environmental worldview of the community and explains the formulation of the attitudes by the local households towards species in the environment. The study uses two horizontal dimensions of environmental attitudes formulation (New Environmental Paradigm (NEP) Scale and Kellet’s (1996) classification of environmental values). Data was collected using a questionnaire survey, group discussions and key informant interviews. A sample of 156 individuals comprised the study respondents. Results showed the residents of rural Gwanda district to hold both a conservation and utilisation conviction (syncretic view) towards the environment. However, utilisation outweighs conservation. Furthermore, older residents are more inclined to conservation in comparison to the youth. The study also divulged that the origin of a species in the natural environment was insignificant to the host community. However, the livelihood effects that species had (regardless of origins) were the major determinants of attitudes developed towards it.
- Full Text:
- Authors: Dube, Nqobizitha , Snowball, Jeanette D , Fraser, Gavin C G
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/68578 , vital:29289 , http://2015.essa.org.za/fullpaper/essa_2994.pdf
- Description: Publisher version , Community environmental perceptions are instrumental in environmental management programmes given that perspectives govern human-environment relations. Despite numerous studies on environmental perceptions, little is known about how the rural poor particularly in Africa conceptualize, live with, and respond to pressing environmental issues facing them. As such, this paper uses the case of an invasive alien plant (IAP) (Cylindropuntia fulgida var. fulgida (Cff)) in a rural community (Gwanda district, Zimbabwe) to unveil the conceptualisation of the natural environment from a rural African perspective. This paper discloses the environmental worldview of the community and explains the formulation of the attitudes by the local households towards species in the environment. The study uses two horizontal dimensions of environmental attitudes formulation (New Environmental Paradigm (NEP) Scale and Kellet’s (1996) classification of environmental values). Data was collected using a questionnaire survey, group discussions and key informant interviews. A sample of 156 individuals comprised the study respondents. Results showed the residents of rural Gwanda district to hold both a conservation and utilisation conviction (syncretic view) towards the environment. However, utilisation outweighs conservation. Furthermore, older residents are more inclined to conservation in comparison to the youth. The study also divulged that the origin of a species in the natural environment was insignificant to the host community. However, the livelihood effects that species had (regardless of origins) were the major determinants of attitudes developed towards it.
- Full Text:
Performance management of health professionals : an evaluation research study of health services in the subdistrict of Buffalo City in the Provincial Department of Health in the Eastern Cape
- Authors: Booi, Mlungisi Wellington
- Date: 2015
- Subjects: South Africa -- Department of Health , Batho Pele (Program) , Public health personnel -- South Africa -- Buffalo City , Employees -- Rating of -- South Africa -- Buffalo City , Public health -- South Africa -- Buffalo City -- Evaluation , Customer services -- South Africa -- Buffalo City -- Evaluation , Employees -- Training of -- South Africa -- Buffalo City , Employee-management relations in government -- South Africa -- Buffalo City
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:859 , http://hdl.handle.net/10962/d1020017
- Description: Integrative Summary In 1997 the Minister of Public Service and Administration of South Africa, Zola Skweyiya, introduced the White Paper on Transforming Public Service Delivery(Department of Public Service and Administration(DPSA,1997: 1). It was premised on what was called Batho Pele (DPSA, 1997: 9), giving effect to Section 197 of the Constitution, Act 108 of 1996 as amended. A linkage to performance management can be found from the assertion made in the White Paper that in future the Batho Pele would form the basis of any assessment of the performance of individual staff and that would contribute to improving the delivery of service (DPSA, 1997:16). Another factor that contributed to the utilisation of a performance management system was the recommendation put forward by the Public Service Commission of South Africa( PSC,2004: 16),that called for public institutions to adopt a clear, comprehensive performance management and evaluation framework for the delivery of public service. That was expected to turn around the ailing public institutions whose record of serving the public with pride and dignity was on the decline. The research aim was to evaluate the effectiveness of the performance management system in creating and resolving role conflict with health professionals employed by the Buffalo City sub-district. The research goal was divided into three objectives, namely: evaluation of the effectiveness of the performance management system as a managerial tool for health managers to manage their subordinates including both professional and support staff, evaluation of the effectiveness of the performance system from the perspective of the health professional as a subordinate and to analyze the functioning and effectiveness of the system in creating and resolving role conflict between professional conduct and organisational requirements. The respondents noted that the implementation of the system was not matched with appointment at managerial level with officials having relevant managerial capacity. Further to that resources at the coal face of service delivery continued to deteriorate and became very scarce and there is no documented proof that has been found of an effort made to check the compatibility of the system to health professionals. The literature identified negative effects that have emanated from the implementation of the performance management system. The study was conducted by interviewing health professionals from the entry level to the management level within the Buffalo City sub-district from three mini hospitals or Health Care Centres. The respondents indicated that there are positives that have been achieved by the implementation of the system, such as the skills gap identification as well as specific targets contained in annual performance contracts. These targets help motivate health professional to focus on that particular direction of activities. Unfortunately it has been clearly documented that the performance management system has also contributed to the development of role conflict in a number of professionals. It has been documented that the system has not been crafted with a health professional in mind and, as such, appears to favour quantity rather than quality of health services as advocated by the codes of practice of different health professionals. There are a number of recommendations that were put forward by the respondents to try to salvage the system and in mitigation of the identified negative factors. For the improvement of the system, the Eastern Cape Provincial Department of Health has to firstly customise the annual contracts to contain only the fundamental information. Secondly need to improve the transparency pertaining to financial pressures facing the Health Department. Thirdly the provision of essential enablers, need to be prioritised. Fourthly there is a need to upgrade the system to incorporate 360 degree feedback. Further research recommendations include a bigger sample incorporating different research methods and to also incorporate searches for measures that can be implemented to improve the system to be more relevant to health professionals. This document is organised and presented in three sections. The first section is the evaluation report with a review of literature, research methodology, findings, discussion and conclusion. The second section is where an indepth literature review is located and the last section details the research methodology.
- Full Text:
- Authors: Booi, Mlungisi Wellington
- Date: 2015
- Subjects: South Africa -- Department of Health , Batho Pele (Program) , Public health personnel -- South Africa -- Buffalo City , Employees -- Rating of -- South Africa -- Buffalo City , Public health -- South Africa -- Buffalo City -- Evaluation , Customer services -- South Africa -- Buffalo City -- Evaluation , Employees -- Training of -- South Africa -- Buffalo City , Employee-management relations in government -- South Africa -- Buffalo City
- Language: English
- Type: Thesis , Masters , MBA
- Identifier: vital:859 , http://hdl.handle.net/10962/d1020017
- Description: Integrative Summary In 1997 the Minister of Public Service and Administration of South Africa, Zola Skweyiya, introduced the White Paper on Transforming Public Service Delivery(Department of Public Service and Administration(DPSA,1997: 1). It was premised on what was called Batho Pele (DPSA, 1997: 9), giving effect to Section 197 of the Constitution, Act 108 of 1996 as amended. A linkage to performance management can be found from the assertion made in the White Paper that in future the Batho Pele would form the basis of any assessment of the performance of individual staff and that would contribute to improving the delivery of service (DPSA, 1997:16). Another factor that contributed to the utilisation of a performance management system was the recommendation put forward by the Public Service Commission of South Africa( PSC,2004: 16),that called for public institutions to adopt a clear, comprehensive performance management and evaluation framework for the delivery of public service. That was expected to turn around the ailing public institutions whose record of serving the public with pride and dignity was on the decline. The research aim was to evaluate the effectiveness of the performance management system in creating and resolving role conflict with health professionals employed by the Buffalo City sub-district. The research goal was divided into three objectives, namely: evaluation of the effectiveness of the performance management system as a managerial tool for health managers to manage their subordinates including both professional and support staff, evaluation of the effectiveness of the performance system from the perspective of the health professional as a subordinate and to analyze the functioning and effectiveness of the system in creating and resolving role conflict between professional conduct and organisational requirements. The respondents noted that the implementation of the system was not matched with appointment at managerial level with officials having relevant managerial capacity. Further to that resources at the coal face of service delivery continued to deteriorate and became very scarce and there is no documented proof that has been found of an effort made to check the compatibility of the system to health professionals. The literature identified negative effects that have emanated from the implementation of the performance management system. The study was conducted by interviewing health professionals from the entry level to the management level within the Buffalo City sub-district from three mini hospitals or Health Care Centres. The respondents indicated that there are positives that have been achieved by the implementation of the system, such as the skills gap identification as well as specific targets contained in annual performance contracts. These targets help motivate health professional to focus on that particular direction of activities. Unfortunately it has been clearly documented that the performance management system has also contributed to the development of role conflict in a number of professionals. It has been documented that the system has not been crafted with a health professional in mind and, as such, appears to favour quantity rather than quality of health services as advocated by the codes of practice of different health professionals. There are a number of recommendations that were put forward by the respondents to try to salvage the system and in mitigation of the identified negative factors. For the improvement of the system, the Eastern Cape Provincial Department of Health has to firstly customise the annual contracts to contain only the fundamental information. Secondly need to improve the transparency pertaining to financial pressures facing the Health Department. Thirdly the provision of essential enablers, need to be prioritised. Fourthly there is a need to upgrade the system to incorporate 360 degree feedback. Further research recommendations include a bigger sample incorporating different research methods and to also incorporate searches for measures that can be implemented to improve the system to be more relevant to health professionals. This document is organised and presented in three sections. The first section is the evaluation report with a review of literature, research methodology, findings, discussion and conclusion. The second section is where an indepth literature review is located and the last section details the research methodology.
- Full Text:
Performing whiteness; representing otherness : Hugh Tracey and African music
- Authors: Coetzee, Paulette June
- Date: 2015
- Subjects: Tracey, Hugh , Music -- Africa , International Library of African Music , Ethnomusicology -- Africa , Ethnomusicologists -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2327 , http://hdl.handle.net/10962/d1016502
- Description: This thesis provides a critical study of texts associated with Hugh Tracey (1903–1977). Tracey is well-known for his work in African music studies, particularly for his major contribution to the recorded archive of musical sound in sub-Saharan Africa and his founding of the International Library of African Music (ILAM) in 1954. My reading of him is informed by a postcolonial perspective, whiteness studies and African scholarship on ways in which constructions of African identity and tradition have been shaped by the colonial archive. In my view, Tracey was part of a mid-twentieth century movement which sought to marshal positive representations of traditional African culture in the interest of maintaining and strengthening colonial rule. While his recording project may have fostered inclusion through creating spaces for indigenous musicians to be heard, it also functioned to promote racist exclusion in the manner of its production, distribution and claims to expertise. Moreover, his initial strategy for ILAM’s sustainability targeted colonial government and industry as primary clients, with the promise that promoting traditional music as a means of entertainment and self-expression for black subjects and workers would ease administration and reduce conflict. I believe that it is important to acknowledge and interrogate the problematic racial attitudes and practices associated with the history of Tracey’s archive – not to undermine its significance in any way but to allow it to be better understood and used more productively in the future.
- Full Text:
- Authors: Coetzee, Paulette June
- Date: 2015
- Subjects: Tracey, Hugh , Music -- Africa , International Library of African Music , Ethnomusicology -- Africa , Ethnomusicologists -- South Africa
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:2327 , http://hdl.handle.net/10962/d1016502
- Description: This thesis provides a critical study of texts associated with Hugh Tracey (1903–1977). Tracey is well-known for his work in African music studies, particularly for his major contribution to the recorded archive of musical sound in sub-Saharan Africa and his founding of the International Library of African Music (ILAM) in 1954. My reading of him is informed by a postcolonial perspective, whiteness studies and African scholarship on ways in which constructions of African identity and tradition have been shaped by the colonial archive. In my view, Tracey was part of a mid-twentieth century movement which sought to marshal positive representations of traditional African culture in the interest of maintaining and strengthening colonial rule. While his recording project may have fostered inclusion through creating spaces for indigenous musicians to be heard, it also functioned to promote racist exclusion in the manner of its production, distribution and claims to expertise. Moreover, his initial strategy for ILAM’s sustainability targeted colonial government and industry as primary clients, with the promise that promoting traditional music as a means of entertainment and self-expression for black subjects and workers would ease administration and reduce conflict. I believe that it is important to acknowledge and interrogate the problematic racial attitudes and practices associated with the history of Tracey’s archive – not to undermine its significance in any way but to allow it to be better understood and used more productively in the future.
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PFB0595w is a Plasmodium falciparum J protein that co-localizes with PfHsp70-1 and can stimulate its in vitro ATP hydrolysis activity
- Njunge, James M, Mandal, Pradipta, Przyborski, Jude M, Boshoff, Aileen, Pesce, Eva-Rachele, Blatch, Gregory L
- Authors: Njunge, James M , Mandal, Pradipta , Przyborski, Jude M , Boshoff, Aileen , Pesce, Eva-Rachele , Blatch, Gregory L
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/431739 , vital:72800 , xlink:href="https://doi.org/10.1016/j.biocel.2015.02.008"
- Description: Heat shock proteins, many of which function as molecular chaperones, play important roles in the lifecycle and pathogenesis of the malaria parasite, Plasmodium falciparum. The P. falciparum heat shock protein 70 (PfHsp70) family of chaperones is potentially regulated by a large complement of J proteins that localize to various intracellular compartments including the infected erythrocyte cytosol. While PfHsp70-1 has been shown to be an abundant cytosolic chaperone, its regulation by J proteins is poorly understood. In this study, we characterized the J protein PFB0595w, a homologue of the well-studied yeast cytosolic J protein, Sis1. PFB0595w, similarly to PfHsp70-1, was localized to the parasite cytosol and its expression was upregulated by heat shock. Additionally, recombinant PFB0595w was shown to be dimeric and to stimulate the in vitro ATPase activity of PfHsp70-1. Overall, the expression, localization and biochemical data for PFB0595w suggest that it may function as a cochaperone of PfHsp70-1, and advances current knowledge on the chaperone machinery of the parasite.
- Full Text:
- Authors: Njunge, James M , Mandal, Pradipta , Przyborski, Jude M , Boshoff, Aileen , Pesce, Eva-Rachele , Blatch, Gregory L
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/431739 , vital:72800 , xlink:href="https://doi.org/10.1016/j.biocel.2015.02.008"
- Description: Heat shock proteins, many of which function as molecular chaperones, play important roles in the lifecycle and pathogenesis of the malaria parasite, Plasmodium falciparum. The P. falciparum heat shock protein 70 (PfHsp70) family of chaperones is potentially regulated by a large complement of J proteins that localize to various intracellular compartments including the infected erythrocyte cytosol. While PfHsp70-1 has been shown to be an abundant cytosolic chaperone, its regulation by J proteins is poorly understood. In this study, we characterized the J protein PFB0595w, a homologue of the well-studied yeast cytosolic J protein, Sis1. PFB0595w, similarly to PfHsp70-1, was localized to the parasite cytosol and its expression was upregulated by heat shock. Additionally, recombinant PFB0595w was shown to be dimeric and to stimulate the in vitro ATPase activity of PfHsp70-1. Overall, the expression, localization and biochemical data for PFB0595w suggest that it may function as a cochaperone of PfHsp70-1, and advances current knowledge on the chaperone machinery of the parasite.
- Full Text:
Photo-physicochemical studies and photodynamic therapy activity of indium and gallium phthalocyanines
- Tshangana, Charmaine Sesethu
- Authors: Tshangana, Charmaine Sesethu
- Date: 2015
- Subjects: Quantum dots , Nanoparticles , Photochemotherapy , Phthalocyanines
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4548 , http://hdl.handle.net/10962/d1017928
- Description: The potential toxicity of seven different types of quantum dots without shell (L-cysteine-CdTe, TGA-CdTe, MPA-CdTe, TGA-CdSe) and with the shell (GSH-CdSe@ZnS, GSH-CdTe@ZnS,) with different capping agents were evaluated. The growth inhibitory effects of the various quantum dots on human pancreatic BON cancerous cells were determined. The least cytotoxic of the various quantum dots synthesized and the one displaying the lowest growth inhibitory potential and no embryotoxicity was determined to be the GSH-CdSe@ZnS quantum dots. The GSH-CdSe@ZnS quantum dots were then conjugated to gallium, aluminium and indium octacarboxy phthalocyanine and the photophysical behaviour of the conjugates studied for potential use in photodynamic therapy and imaging applications. The sizes, morphology, thermal stability and confirmation of successful conjugation was determined using X-ray diffraction (XRD), transmission electron microscopy (TEM), thermogravimetric analysis (TGA) and Fourier transform infrared spectroscopy (FTIR), respectively. The study was extended by conjugating amino functionalized magnetic nanoparticles (Fe₃O₄) to indium octacarboxy phthalocyanine to study the photophysical behaviour of the conjugate as a potential bi-functional anti-cancer agent (hyperthermia and photodynamic therapy applications). A three-in-one multifunctional nanocomposite comprising of the quantum dots, magnetic nanoparticles and indium octacarboxy phthalocyanine was developed with the aim of developing a multifunctional composite that is able detect, monitor and treat cancer. All conjugates showed improved and enhanced photophysical behaviour. Finally, GSH-CdSe@ZnS conjugated to aluminium octacarboxy phthalocyanine was applied in human pancreatic carcinoid BON cells. The conjugates induced cell death dose-dependently.
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- Authors: Tshangana, Charmaine Sesethu
- Date: 2015
- Subjects: Quantum dots , Nanoparticles , Photochemotherapy , Phthalocyanines
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:4548 , http://hdl.handle.net/10962/d1017928
- Description: The potential toxicity of seven different types of quantum dots without shell (L-cysteine-CdTe, TGA-CdTe, MPA-CdTe, TGA-CdSe) and with the shell (GSH-CdSe@ZnS, GSH-CdTe@ZnS,) with different capping agents were evaluated. The growth inhibitory effects of the various quantum dots on human pancreatic BON cancerous cells were determined. The least cytotoxic of the various quantum dots synthesized and the one displaying the lowest growth inhibitory potential and no embryotoxicity was determined to be the GSH-CdSe@ZnS quantum dots. The GSH-CdSe@ZnS quantum dots were then conjugated to gallium, aluminium and indium octacarboxy phthalocyanine and the photophysical behaviour of the conjugates studied for potential use in photodynamic therapy and imaging applications. The sizes, morphology, thermal stability and confirmation of successful conjugation was determined using X-ray diffraction (XRD), transmission electron microscopy (TEM), thermogravimetric analysis (TGA) and Fourier transform infrared spectroscopy (FTIR), respectively. The study was extended by conjugating amino functionalized magnetic nanoparticles (Fe₃O₄) to indium octacarboxy phthalocyanine to study the photophysical behaviour of the conjugate as a potential bi-functional anti-cancer agent (hyperthermia and photodynamic therapy applications). A three-in-one multifunctional nanocomposite comprising of the quantum dots, magnetic nanoparticles and indium octacarboxy phthalocyanine was developed with the aim of developing a multifunctional composite that is able detect, monitor and treat cancer. All conjugates showed improved and enhanced photophysical behaviour. Finally, GSH-CdSe@ZnS conjugated to aluminium octacarboxy phthalocyanine was applied in human pancreatic carcinoid BON cells. The conjugates induced cell death dose-dependently.
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Photocatalytic behaviour of zinc tetraamino phthalocyanine-silver nanoparticles immobilized on chitosan beads
- Khoza, Phindile, Nyokong, Tebello
- Authors: Khoza, Phindile , Nyokong, Tebello
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/189468 , vital:44849 , xlink:href="https://doi.org/10.1016/j.molcata.2015.01.017"
- Description: Photocatalytic degradation of Rhodamine 6G using unconjugated zinc tetraamino phthalocyanine (ZnTAPc) or when conjugated to Ag nanoparticles (ZnTAPc–AgNPs) is reported. Upon conjugating ZnTAPc to silver nanoparticles, the singlet oxygen production of the phthalocyanine was slightly increased. ZnTAPc and ZnTAPc–AgNPs were immobilized onto chitosan beads for ease of recovery after photocatalysis. Chitosan beads were characterized by FTIR, XRD and TGA. The photodegradation of Rhodamine 6G was used to evaluate the efficiency of the immobilized photocatalysts. In the presence of AgNPs, the photodegradation of Rhodamine 6G was enhanced. The apparent rates (k) were found to be 8.51 × 10−8 and 1.61 × 10−7 mol L−1 min−1 for chitosan supported ZnTAPc or ZnTAPc–AgNPs, respectively. The observation of good photocatalytic activity of the ZnTAPc when immobilized on chitosan proves the uncompromised efficiency of the photocatalysts even when confined in beads, showing great potential for the functionalized beads as heterogeneous catalysts.
- Full Text:
- Authors: Khoza, Phindile , Nyokong, Tebello
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/189468 , vital:44849 , xlink:href="https://doi.org/10.1016/j.molcata.2015.01.017"
- Description: Photocatalytic degradation of Rhodamine 6G using unconjugated zinc tetraamino phthalocyanine (ZnTAPc) or when conjugated to Ag nanoparticles (ZnTAPc–AgNPs) is reported. Upon conjugating ZnTAPc to silver nanoparticles, the singlet oxygen production of the phthalocyanine was slightly increased. ZnTAPc and ZnTAPc–AgNPs were immobilized onto chitosan beads for ease of recovery after photocatalysis. Chitosan beads were characterized by FTIR, XRD and TGA. The photodegradation of Rhodamine 6G was used to evaluate the efficiency of the immobilized photocatalysts. In the presence of AgNPs, the photodegradation of Rhodamine 6G was enhanced. The apparent rates (k) were found to be 8.51 × 10−8 and 1.61 × 10−7 mol L−1 min−1 for chitosan supported ZnTAPc or ZnTAPc–AgNPs, respectively. The observation of good photocatalytic activity of the ZnTAPc when immobilized on chitosan proves the uncompromised efficiency of the photocatalysts even when confined in beads, showing great potential for the functionalized beads as heterogeneous catalysts.
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