Historical demography of southern African patellid limpets: congruence of population expansions, but not phylogeography
- Mmonwa, Kolobe L, Teske, Peter R, McQuaid, Christopher D, Barker, Nigel P
- Authors: Mmonwa, Kolobe L , Teske, Peter R , McQuaid, Christopher D , Barker, Nigel P
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/444814 , vital:74300 , https://doi.org/10.2989/1814232X.2015.1009165
- Description: Global climatic oscillations have shaped the contemporary genetic structure of marine taxa in different ways. Previous demographic studies have indicated that various intertidal marine species display genetic signatures of demographic expansion that either pre- or postdate the Last Glacial Maximum. Such expansions and the ability of species to colonise new habitats will influence their genetic structure, but the link between scales of larval dispersal and the strength of phylogeographic structure is not always clear. We analysed a fragment of the mitochondrial COI gene of 11 sympatric species of intertidal southern African patellid limpets to investigate how ancient oceanographic dynamics have shaped and maintained their contemporary spatial genetic variation. Our data show that the patellid limpets investigated display congruent evidence of spatial expansion during the Late Pleistocene or Early Holocene, which corresponds with the establishment of the contemporary southern African shoreline. We argue that closely related and co-distributed southern African intertidal invertebrates responded to ancient climatic oscillations as a cohesive group. In contrast, contemporary oceanographic circulation has shaped the phylogeographic patterns of these limpets in different ways. We show close relationships between phylogeography and biogeography for some species, but not for others, despite the similarities in their life histories and exposure to the same climatic changes.
- Full Text:
- Date Issued: 2015
- Authors: Mmonwa, Kolobe L , Teske, Peter R , McQuaid, Christopher D , Barker, Nigel P
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/444814 , vital:74300 , https://doi.org/10.2989/1814232X.2015.1009165
- Description: Global climatic oscillations have shaped the contemporary genetic structure of marine taxa in different ways. Previous demographic studies have indicated that various intertidal marine species display genetic signatures of demographic expansion that either pre- or postdate the Last Glacial Maximum. Such expansions and the ability of species to colonise new habitats will influence their genetic structure, but the link between scales of larval dispersal and the strength of phylogeographic structure is not always clear. We analysed a fragment of the mitochondrial COI gene of 11 sympatric species of intertidal southern African patellid limpets to investigate how ancient oceanographic dynamics have shaped and maintained their contemporary spatial genetic variation. Our data show that the patellid limpets investigated display congruent evidence of spatial expansion during the Late Pleistocene or Early Holocene, which corresponds with the establishment of the contemporary southern African shoreline. We argue that closely related and co-distributed southern African intertidal invertebrates responded to ancient climatic oscillations as a cohesive group. In contrast, contemporary oceanographic circulation has shaped the phylogeographic patterns of these limpets in different ways. We show close relationships between phylogeography and biogeography for some species, but not for others, despite the similarities in their life histories and exposure to the same climatic changes.
- Full Text:
- Date Issued: 2015
Hit them where it hurts-tackling Facebook's misogyny problem: journalism next
- Authors: Roux, Kayla
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/454745 , vital:75372 , https://hdl.handle.net/10520/EJC175783
- Description: Whether you believe in the power of social networking for political par-ticipation or are cynical of the 'clicktivists' and their 'slacktivism', there's no denying it: social media politics have become an inescapable part of our digital lives. From online petitions and NGO fan pages to heated Twitter wars and politically-motivated hacking, the internet has opened up countless new avenues in which people can express their support for causes, lobby powerful interest groups, and register their dissent with a well-orchestrated hashtag or viral campaign. But what if one so-cial network becomes the site of the struggle? What about when our digital lives become the subject of our politics?
- Full Text:
- Date Issued: 2015
- Authors: Roux, Kayla
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/454745 , vital:75372 , https://hdl.handle.net/10520/EJC175783
- Description: Whether you believe in the power of social networking for political par-ticipation or are cynical of the 'clicktivists' and their 'slacktivism', there's no denying it: social media politics have become an inescapable part of our digital lives. From online petitions and NGO fan pages to heated Twitter wars and politically-motivated hacking, the internet has opened up countless new avenues in which people can express their support for causes, lobby powerful interest groups, and register their dissent with a well-orchestrated hashtag or viral campaign. But what if one so-cial network becomes the site of the struggle? What about when our digital lives become the subject of our politics?
- Full Text:
- Date Issued: 2015
Horns of dilemma : Department head and subject teacher : a case study of Heads of Department in a public secondary school, northern Namibia
- Authors: Naundobe, Robert Natukondye
- Date: 2015
- Subjects: High school department heads -- Namibia , High school teachers -- Workload -- Namibia , Teacher effectiveness -- Namibia , Educational leadership -- Namibia , Conflict of interests
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:2043 , http://hdl.handle.net/10962/d1017354
- Description: The accountability and responsibilities of school leaders have intensified greatly over the past decades and school leadership has become a strong focus of research. Meanwhile, Bush (2003) asserts that “school leaders [experience] tensions between competing elements of leadership, management and administration” (p. 7). This study uses observations, questionnaires, interviews and document analysis to unpack the perceptions of four Heads of Department (HoDs), the principal, and eight teachers on the tensions inherent in balancing the responsibilities of department head and subject teacher for HoDs in public secondary school. The study goes beyond the mere task of influence in its attempts to unpack how the leadership and teaching practices of HoDs may, or may not, be in conflict with each other. Using distributed leadership as a theoretical framing and drawing in particular on the work of Spillane and colleagues (2001; 2004), the study examines the roles HoDs enact; the challenges HoDs encounter in enacting their responsibilities as department head and subject teacher; and the strategies HoDs employ to combat the emerging challenges. The study found that the roles of HoDs are extensive and stretch across the classroom, the department, the whole school and beyond. However, the majority of these roles are biased in favour of management systems and processes and opportunities for leadership are rare. The many and extensive management responsibilities of the HoDs limit both their classroom teaching as well as their agency as leaders. The weight of their management work thus restricts their leadership, resulting in an authorised form of distributed leadership (Grant, 2010). The data also revealed that HoDs struggle to balance the responsibilities of department head and subject teacher due to both inter-role and intra-sender conflict. However, the study also found that the HoDs strategically adopt a range of strategies to assist them in doing their work, these include: compensatory teaching; delegation; and planning and prioritizing.
- Full Text:
- Date Issued: 2015
- Authors: Naundobe, Robert Natukondye
- Date: 2015
- Subjects: High school department heads -- Namibia , High school teachers -- Workload -- Namibia , Teacher effectiveness -- Namibia , Educational leadership -- Namibia , Conflict of interests
- Language: English
- Type: Thesis , Masters , MEd
- Identifier: vital:2043 , http://hdl.handle.net/10962/d1017354
- Description: The accountability and responsibilities of school leaders have intensified greatly over the past decades and school leadership has become a strong focus of research. Meanwhile, Bush (2003) asserts that “school leaders [experience] tensions between competing elements of leadership, management and administration” (p. 7). This study uses observations, questionnaires, interviews and document analysis to unpack the perceptions of four Heads of Department (HoDs), the principal, and eight teachers on the tensions inherent in balancing the responsibilities of department head and subject teacher for HoDs in public secondary school. The study goes beyond the mere task of influence in its attempts to unpack how the leadership and teaching practices of HoDs may, or may not, be in conflict with each other. Using distributed leadership as a theoretical framing and drawing in particular on the work of Spillane and colleagues (2001; 2004), the study examines the roles HoDs enact; the challenges HoDs encounter in enacting their responsibilities as department head and subject teacher; and the strategies HoDs employ to combat the emerging challenges. The study found that the roles of HoDs are extensive and stretch across the classroom, the department, the whole school and beyond. However, the majority of these roles are biased in favour of management systems and processes and opportunities for leadership are rare. The many and extensive management responsibilities of the HoDs limit both their classroom teaching as well as their agency as leaders. The weight of their management work thus restricts their leadership, resulting in an authorised form of distributed leadership (Grant, 2010). The data also revealed that HoDs struggle to balance the responsibilities of department head and subject teacher due to both inter-role and intra-sender conflict. However, the study also found that the HoDs strategically adopt a range of strategies to assist them in doing their work, these include: compensatory teaching; delegation; and planning and prioritizing.
- Full Text:
- Date Issued: 2015
Host searching and oviposition behaviour of Agathis bishopi (Hymenoptera: Braconidae), a larval parasitoid of false codling moth, Thaumatotibia leucotreta (Lepidoptera: Tortricidae): a potential proxy indicator for fruit infestation
- Zimba, Kennedy J, Heshula, Lelethu U P, Moore, Sean D, Hill, Martin P
- Authors: Zimba, Kennedy J , Heshula, Lelethu U P , Moore, Sean D , Hill, Martin P
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/407019 , vital:70330 , xlink:href="https://hdl.handle.net/10520/EJC195073"
- Description: Agathis bishopi (Nixon) (Hymenoptera: Braconidae)is an arrhenotokous larval endoparasitoid of Thaumatotibia leucotreta (Meyrick) (Lepidoptera: Tortricidae) (Gendall 2007; Hofmeyr et al. 2015) commonly known as false codling moth (FCM), a major pest of citrus in South Africa (Moore et al. 2004; Malan et al. 2011). Under field conditions, A. bishopi was identified attacking more than 34% of FCM larvae in fruit, showing good biocontrol potential (Gendall 2007). Preference by A. bishopi for parasitising the early instars of its concealed host suggests that the parasitoid has strong natural host location ability (Sishuba 2003; Gendall 2007).
- Full Text:
- Date Issued: 2015
- Authors: Zimba, Kennedy J , Heshula, Lelethu U P , Moore, Sean D , Hill, Martin P
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/407019 , vital:70330 , xlink:href="https://hdl.handle.net/10520/EJC195073"
- Description: Agathis bishopi (Nixon) (Hymenoptera: Braconidae)is an arrhenotokous larval endoparasitoid of Thaumatotibia leucotreta (Meyrick) (Lepidoptera: Tortricidae) (Gendall 2007; Hofmeyr et al. 2015) commonly known as false codling moth (FCM), a major pest of citrus in South Africa (Moore et al. 2004; Malan et al. 2011). Under field conditions, A. bishopi was identified attacking more than 34% of FCM larvae in fruit, showing good biocontrol potential (Gendall 2007). Preference by A. bishopi for parasitising the early instars of its concealed host suggests that the parasitoid has strong natural host location ability (Sishuba 2003; Gendall 2007).
- Full Text:
- Date Issued: 2015
How can conversational analysis contribute to ‘doing’ critical work?: extending the methodological conversation
- Authors: du Toit, Ryan
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/143904 , vital:38293 , https://ischp.files.wordpress.com/2015/08/ischp_2015_abstract_booklet.pdf
- Description: Conversation analysis (CA) has been criticised as being overly obsessed with the intricate details of conversation and bracketing off the wider socio-structural power relations in which conversation is embedded. Many scholars regard CA as a methodology that is incompatible with, and thus incapable of doing, critical work. This paper seeks to contribute to the methodological conversation by proposing a dual analytical methodology that merges understandings of talk from CA and critical discursive approaches, and attends to both the conversational (turn-by-turn) context as well as the discursive resources that inhabit talk. This is accomplished through a triangulatory activity that takes different conceptualizations of ‘context’ into consideration and investigates how subject positions are worked up on a micro- (turnby-turn) and macro-level. The authors illustrate this approach using institutional talk collected at a UK diabetes clinic and from interviews investigating how employers talk about their domestic workers in South Africa.
- Full Text:
- Date Issued: 2015
- Authors: du Toit, Ryan
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/143904 , vital:38293 , https://ischp.files.wordpress.com/2015/08/ischp_2015_abstract_booklet.pdf
- Description: Conversation analysis (CA) has been criticised as being overly obsessed with the intricate details of conversation and bracketing off the wider socio-structural power relations in which conversation is embedded. Many scholars regard CA as a methodology that is incompatible with, and thus incapable of doing, critical work. This paper seeks to contribute to the methodological conversation by proposing a dual analytical methodology that merges understandings of talk from CA and critical discursive approaches, and attends to both the conversational (turn-by-turn) context as well as the discursive resources that inhabit talk. This is accomplished through a triangulatory activity that takes different conceptualizations of ‘context’ into consideration and investigates how subject positions are worked up on a micro- (turnby-turn) and macro-level. The authors illustrate this approach using institutional talk collected at a UK diabetes clinic and from interviews investigating how employers talk about their domestic workers in South Africa.
- Full Text:
- Date Issued: 2015
How general-purpose can a GPU be?
- Authors: Machanick, Philip
- Date: 2015
- Language: English
- Type: article , text
- Identifier: http://hdl.handle.net/10962/61180 , vital:27988 , http://dx.doi.org/10.18489/sacj.v0i57.347
- Description: The use of graphics processing units (GPUs) in general-purpose computation (GPGPU) is a growing field. GPU instruction sets, while implementing a graphics pipeline, draw from a range of single instruction multiple datastream (SIMD) architectures characteristic of the heyday of supercomputers. Yet only one of these SIMD instruction sets has been of application on a wide enough range of problems to survive the era when the full range of supercomputer design variants was being explored: vector instructions. Supercomputers covered a range of exotic designs such as hypercubes and the Connection Machine (Fox, 1989). The latter is likely the source of the snide comment by Cray: it had thousands of relatively low-speed CPUs (Tucker & Robertson, 1988). Since Cray won, why are we not basing our ideas on his designs (Cray Inc., 2004), rather than those of the losers? The Top 500 supercomputer list is dominated by general-purpose CPUs, and nothing like the Connection Machine that headed the list in 1993 still exists.
- Full Text:
- Date Issued: 2015
- Authors: Machanick, Philip
- Date: 2015
- Language: English
- Type: article , text
- Identifier: http://hdl.handle.net/10962/61180 , vital:27988 , http://dx.doi.org/10.18489/sacj.v0i57.347
- Description: The use of graphics processing units (GPUs) in general-purpose computation (GPGPU) is a growing field. GPU instruction sets, while implementing a graphics pipeline, draw from a range of single instruction multiple datastream (SIMD) architectures characteristic of the heyday of supercomputers. Yet only one of these SIMD instruction sets has been of application on a wide enough range of problems to survive the era when the full range of supercomputer design variants was being explored: vector instructions. Supercomputers covered a range of exotic designs such as hypercubes and the Connection Machine (Fox, 1989). The latter is likely the source of the snide comment by Cray: it had thousands of relatively low-speed CPUs (Tucker & Robertson, 1988). Since Cray won, why are we not basing our ideas on his designs (Cray Inc., 2004), rather than those of the losers? The Top 500 supercomputer list is dominated by general-purpose CPUs, and nothing like the Connection Machine that headed the list in 1993 still exists.
- Full Text:
- Date Issued: 2015
How General-Purpose can a GPU be?
- Authors: Machanick, Philip
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/439294 , vital:73563 , https://hdl.handle.net/10520/EJC181693
- Description: The use of graphics processing units (GPUs) in general-purpose computation (GPGPU) is a growing field. GPU instruction sets, while implementing a graphics pipeline, draw from a range of single instruction multiple data stream (SIMD) architectures characteristic of the heyday of supercomputers. Yet only one of these SIMD instruction sets has been of application on a wide enough range of problems to survive the era when the full range of supercomputer design variants was being explored: vector instructions.
- Full Text:
- Date Issued: 2015
- Authors: Machanick, Philip
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/439294 , vital:73563 , https://hdl.handle.net/10520/EJC181693
- Description: The use of graphics processing units (GPUs) in general-purpose computation (GPGPU) is a growing field. GPU instruction sets, while implementing a graphics pipeline, draw from a range of single instruction multiple data stream (SIMD) architectures characteristic of the heyday of supercomputers. Yet only one of these SIMD instruction sets has been of application on a wide enough range of problems to survive the era when the full range of supercomputer design variants was being explored: vector instructions.
- Full Text:
- Date Issued: 2015
Hsp70/Hsp90 organising protein (hop): beyond interactions with chaperones and prion proteins
- Baindur-Hudson, Swati, Edkins, Adrienne L, Blatch, Gregory L
- Authors: Baindur-Hudson, Swati , Edkins, Adrienne L , Blatch, Gregory L
- Date: 2015
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/164852 , vital:41178 , ISBN 978-3-319-11730-0 , DOI: 10.1007/978-3-319-11731-7_3
- Description: The Hsp70/Hsp90 organising protein (Hop), also known as stress-inducible protein 1 (STI1), has received considerable attention for diverse cellular functions in both healthy and diseased states. There is extensive evidence that intracellular Hop is a co-chaperone of the major chaperones Hsp70 and Hsp90, playing an important role in the productive folding of Hsp90 client proteins. Consequently, Hop is implicated in a number of key signalling pathways, including aberrant pathways leading to cancer. However, Hop is also secreted and it is now well established that Hop also serves as a receptor for the prion protein, PrPC.
- Full Text:
- Date Issued: 2015
- Authors: Baindur-Hudson, Swati , Edkins, Adrienne L , Blatch, Gregory L
- Date: 2015
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/164852 , vital:41178 , ISBN 978-3-319-11730-0 , DOI: 10.1007/978-3-319-11731-7_3
- Description: The Hsp70/Hsp90 organising protein (Hop), also known as stress-inducible protein 1 (STI1), has received considerable attention for diverse cellular functions in both healthy and diseased states. There is extensive evidence that intracellular Hop is a co-chaperone of the major chaperones Hsp70 and Hsp90, playing an important role in the productive folding of Hsp90 client proteins. Consequently, Hop is implicated in a number of key signalling pathways, including aberrant pathways leading to cancer. However, Hop is also secreted and it is now well established that Hop also serves as a receptor for the prion protein, PrPC.
- Full Text:
- Date Issued: 2015
Human FN1 is regulated by the heat-shock response
- Authors: Dhanani, Karim Colin Hassan
- Date: 2015
- Subjects: Uncatalogued
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/193487 , vital:45336
- Description: Heat shock protein 90 (Hsp90) and heat shock factors (HSFs) are known to be involved in the epigenetic regulation of several fundamental oncogenic genes. Fibronectin (FN) is an extracellular matrix (ECM) glycoprotein which plays key roles in cell adhesion and migration. Hsp90 binds directly to FN and Hsp90 inhibition has been shown to regulate FN protein levels and matrix formation. Where inhibition of Hsp90 with a C-terminal inhibitor (novobiocin) induced the loss of FN matrix, treatment with an N-terminal inhibitor (geldanamycin) increased FN matrix levels. GA treatment induced a strong dose and time dependent increase in FN1 promoter activity and increased total FN mRNA respectively. By contrast, NOV showed no increase in the promoter activity and no change in the expression of FN mRNA. As GA is known to induce the stress response, we investigated the relationship between the cell stress machinery and the transcriptional regulation of FN. Three putative heat shock elements (HSEs) were identified in the FN1 promoter. The loss of two of the three identified putative HSEs resulted in a loss in the basal transcriptional activity of the FN1 promoter in our reporter model. This was in addition to the loss of the induction of transcriptional activity with GA treatment observed with the full-length promoter. Binding of HSF1 to one of the putative HSEs, which was identified as potentially functional from the truncation analysis, was confirmed using ChIP. The occupancy of this HSE by HSF1 was shown to increase with GA treatment. These data support the hypothesis that FN1 is a functional HSF1 target gene. The 5' promoter regions of seven additional ECM protein encoding genes were analysed and mRNA levels were detected by quantitative RT-PCR upon treatment with GA. Collagen 4 _2 and laminin _3 mRNA were found to increase in the presence of GA, whereas collagen 4 _3 and osteopontin showed no change. Similarly to FN1, these data indicate that a subset of ECM genes may be under the regulation of the HSF1 mediated heat-shock response. This may have implications for our understanding of ECM dynamics in cancer, where the clinical application of Hsp90 inhibitors is intended. Additionally, our data provide a poten- tial underpinning for the role of the HSF1 mediated heat-shock response in several fibrotic and metabolic stress related pathologies. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2015
- Full Text:
- Date Issued: 2015
- Authors: Dhanani, Karim Colin Hassan
- Date: 2015
- Subjects: Uncatalogued
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/193487 , vital:45336
- Description: Heat shock protein 90 (Hsp90) and heat shock factors (HSFs) are known to be involved in the epigenetic regulation of several fundamental oncogenic genes. Fibronectin (FN) is an extracellular matrix (ECM) glycoprotein which plays key roles in cell adhesion and migration. Hsp90 binds directly to FN and Hsp90 inhibition has been shown to regulate FN protein levels and matrix formation. Where inhibition of Hsp90 with a C-terminal inhibitor (novobiocin) induced the loss of FN matrix, treatment with an N-terminal inhibitor (geldanamycin) increased FN matrix levels. GA treatment induced a strong dose and time dependent increase in FN1 promoter activity and increased total FN mRNA respectively. By contrast, NOV showed no increase in the promoter activity and no change in the expression of FN mRNA. As GA is known to induce the stress response, we investigated the relationship between the cell stress machinery and the transcriptional regulation of FN. Three putative heat shock elements (HSEs) were identified in the FN1 promoter. The loss of two of the three identified putative HSEs resulted in a loss in the basal transcriptional activity of the FN1 promoter in our reporter model. This was in addition to the loss of the induction of transcriptional activity with GA treatment observed with the full-length promoter. Binding of HSF1 to one of the putative HSEs, which was identified as potentially functional from the truncation analysis, was confirmed using ChIP. The occupancy of this HSE by HSF1 was shown to increase with GA treatment. These data support the hypothesis that FN1 is a functional HSF1 target gene. The 5' promoter regions of seven additional ECM protein encoding genes were analysed and mRNA levels were detected by quantitative RT-PCR upon treatment with GA. Collagen 4 _2 and laminin _3 mRNA were found to increase in the presence of GA, whereas collagen 4 _3 and osteopontin showed no change. Similarly to FN1, these data indicate that a subset of ECM genes may be under the regulation of the HSF1 mediated heat-shock response. This may have implications for our understanding of ECM dynamics in cancer, where the clinical application of Hsp90 inhibitors is intended. Additionally, our data provide a poten- tial underpinning for the role of the HSF1 mediated heat-shock response in several fibrotic and metabolic stress related pathologies. , Thesis (PhD) -- Faculty of Science, Biochemistry and Microbiology, 2015
- Full Text:
- Date Issued: 2015
Human harvesting impacts on managed areas: ecological effects of socially-compatible shellfish reserves
- Aswani, Shankar, Flores, Carola F, Broitman, Bernardo R
- Authors: Aswani, Shankar , Flores, Carola F , Broitman, Bernardo R
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/124664 , vital:35644 , https://doi.10.1007/s11160-014-9376-4
- Description: We examined how human harvesting impacts on managed areas affect the abundance and size distribution of the edible mangrove shellfish Anadara granosa and Polymesoda spp. in the Roviana Lagoon, Solomon Islands. We tested two hypotheses: (1) in areas permanently and temporally closed to human exploitation, abundance and size distribution of these shellfish species is significantly greater than in sites open to exploitation and (2) moderate human disturbance of shell beds, particularly of Polymesoda spp., increases their abundance. Firstly, we studied perceptions of environmental states and processes coupled to foraging and management interventions to assess sociocultural influences on harvesting practices and ascertain the types of management regime that people would consider in a context where poaching and interloping are common practices. Secondly, we compared shellfish abundance and shell size from areas that were permanently protected, temporally reserved for communal harvest, and permanently open for exploitation. Thirdly, drawing from women’s local knowledge, we measured the abundance of Polymesoda spp. in relation to mud compactness in quadrats across the three management regimes. Results showed that both species were significantly more abundant in permanent and temporally closed sites than in open sites. In the mud compactness study, however, while shell abundance was greater in moderately compacted quadrats, there was no statistical relationship between mud compactness and shell abundance within or across the three management regimes. Results suggest that even under the strong impacts of poaching, temporally closed areas have more clams than open areas and are as effective as areas that are permanently closed nominally. The results also suggest that human harvesting regimes can influence the effectiveness of local management decisions and thus are important when designing community-based conservation programs in the Solomon Islands and other Pacific Islands.
- Full Text:
- Date Issued: 2015
- Authors: Aswani, Shankar , Flores, Carola F , Broitman, Bernardo R
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/124664 , vital:35644 , https://doi.10.1007/s11160-014-9376-4
- Description: We examined how human harvesting impacts on managed areas affect the abundance and size distribution of the edible mangrove shellfish Anadara granosa and Polymesoda spp. in the Roviana Lagoon, Solomon Islands. We tested two hypotheses: (1) in areas permanently and temporally closed to human exploitation, abundance and size distribution of these shellfish species is significantly greater than in sites open to exploitation and (2) moderate human disturbance of shell beds, particularly of Polymesoda spp., increases their abundance. Firstly, we studied perceptions of environmental states and processes coupled to foraging and management interventions to assess sociocultural influences on harvesting practices and ascertain the types of management regime that people would consider in a context where poaching and interloping are common practices. Secondly, we compared shellfish abundance and shell size from areas that were permanently protected, temporally reserved for communal harvest, and permanently open for exploitation. Thirdly, drawing from women’s local knowledge, we measured the abundance of Polymesoda spp. in relation to mud compactness in quadrats across the three management regimes. Results showed that both species were significantly more abundant in permanent and temporally closed sites than in open sites. In the mud compactness study, however, while shell abundance was greater in moderately compacted quadrats, there was no statistical relationship between mud compactness and shell abundance within or across the three management regimes. Results suggest that even under the strong impacts of poaching, temporally closed areas have more clams than open areas and are as effective as areas that are permanently closed nominally. The results also suggest that human harvesting regimes can influence the effectiveness of local management decisions and thus are important when designing community-based conservation programs in the Solomon Islands and other Pacific Islands.
- Full Text:
- Date Issued: 2015
Human myiasis in rural South Africa is under-reported
- Kuria, Simon K, Villet, Martin H, Kingu, H J C, Dhaffala, A
- Authors: Kuria, Simon K , Villet, Martin H , Kingu, H J C , Dhaffala, A
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/442425 , vital:73983 , https://www.ajol.info/index.php/samj/article/view/114028
- Description: Background. Myiasis is the infestation of live tissue of humans and other vertebrates by larvae of flies. Worldwide, myiasis of humans is seldom reported, although the trend is gradually changing in some countries. Reports of human myiasis in Africa are few. Several cases of myiasis were recently seen at the Mthatha Hospital Complex, Mthatha, Eastern Cape Province, South Africa (SA).
- Full Text:
- Date Issued: 2015
- Authors: Kuria, Simon K , Villet, Martin H , Kingu, H J C , Dhaffala, A
- Date: 2015
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/442425 , vital:73983 , https://www.ajol.info/index.php/samj/article/view/114028
- Description: Background. Myiasis is the infestation of live tissue of humans and other vertebrates by larvae of flies. Worldwide, myiasis of humans is seldom reported, although the trend is gradually changing in some countries. Reports of human myiasis in Africa are few. Several cases of myiasis were recently seen at the Mthatha Hospital Complex, Mthatha, Eastern Cape Province, South Africa (SA).
- Full Text:
- Date Issued: 2015
Human, vector and parasite Hsp90 proteins: a comparative bioinformatics analysis
- Faya, Ngonidzashe, Penkler, David L, Tastan Bishop, Özlem
- Authors: Faya, Ngonidzashe , Penkler, David L , Tastan Bishop, Özlem
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/148038 , vital:38704 , DOI: 10.1016/j.fob.2015.11.003
- Description: The treatment of protozoan parasitic diseases is challenging, and thus identification and analysis of new drug targets is important. Parasites survive within host organisms, and some need intermediate hosts to complete their life cycle. Changing host environment puts stress on parasites, and often adaptation is accompanied by the expression of large amounts of heat shock proteins (Hsps). Among Hsps, Hsp90 proteins play an important role in stress environments. Yet, there has been little computational research on Hsp90 proteins to analyze them comparatively as potential parasitic drug targets. Here, an attempt was made to gain detailed insights into the differences between host, vector and parasitic Hsp90 proteins by large-scale bioinformatics analysis.
- Full Text:
- Date Issued: 2015
- Authors: Faya, Ngonidzashe , Penkler, David L , Tastan Bishop, Özlem
- Date: 2015
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/148038 , vital:38704 , DOI: 10.1016/j.fob.2015.11.003
- Description: The treatment of protozoan parasitic diseases is challenging, and thus identification and analysis of new drug targets is important. Parasites survive within host organisms, and some need intermediate hosts to complete their life cycle. Changing host environment puts stress on parasites, and often adaptation is accompanied by the expression of large amounts of heat shock proteins (Hsps). Among Hsps, Hsp90 proteins play an important role in stress environments. Yet, there has been little computational research on Hsp90 proteins to analyze them comparatively as potential parasitic drug targets. Here, an attempt was made to gain detailed insights into the differences between host, vector and parasitic Hsp90 proteins by large-scale bioinformatics analysis.
- Full Text:
- Date Issued: 2015
Hydrothermal co-liquefaction of microalgae biomass and coal
- Authors: Charlie, David
- Date: 2015
- Subjects: Microalgae -- Biotechnology Biomass chemicals , Biomass energy
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: http://hdl.handle.net/10948/50087 , vital:42035
- Description: In this research, the objective was to investigate under the chosen liquefaction conditions whether co-liquefaction of coal and microalgae biomass as agglomerates does possess a distinct advantage over the individual liquefaction of microalgae and coal. This was initially done by preparing dry micro algal biomass, followed by the preparation of coal fines and finally coal-algae as agglomerates. The agglomerates were prepared by continuously adsorbing wet concentrated microalgae slurry/solution onto the dry coal fines in order to prepare different ratios of coal-algae agglomerate(s). Ultimate analysis of the starting material (coal fines and the microalgae) as well the agglomerates then followed. According to research, wet microalgae biomass has the potential of acting as a hydrogen donor. By preparing the agglomerates, the rationale was to promote hydrogen supply from the micro algal biomass that has high hydrogen content to the coal with lower hydrogen content. The resulting product was then expected to have fairly higher hydrogen content. However, this was not observed or evident from the elemental analysis results. The results (elemental composition) showed no difference between the agglomerates and the coal. Based on the observed ultimate analysis results, liquefaction experiments were conducted to determine whether hydrogen transfer and the expected synergistic effect between micro algal biomass and coal would occur. The crude oil product was recovered and simulated distillation technique was used for the characterization of the crude oil product. The results we in agreement that higher yields can be achieved during co-liquefaction as compared to the individual liquefaction. A possible synergistic effect that was investigated in this research existed. Liquefaction results provided evidence of higher yields recovery from the agglomerates compared to those of coal fines and microalgae biomass. Although there was higher recovery on the agglomerates, there appears to be a downward trend (decrease) in product recovery with higher loadings of micro algal biomass on to the coal. This means that higher loading of micro algal biomass tend to suppress liquefaction of coal, thus resulting in lower product recovery. While lower loadings tend to effectively facilitate the liquefaction of coal, thus proving that the proposed synergistic effect between the two does exist. Overall product recovery (yields) of the agglomerates is higher compared to the individual recovery of coal fines and microalgae biomass. This was followed by mass balancing to determine the overall conversion. Higher conversions were achieved on the agglomerates compared to coal. This could mean that microalgae have the potential to facilitate the liquefaction of coal and improve its conversion. The observed trend results in improved conversion as well as higher oil yields (simulated distillation results). Overall, the mass balancing provided insightful information regarding the coal-microalgae interaction based on the conversion, and this further corresponds or supports the simulated distillation results. This research paper provides evidence of the synergistic effect that exists between micro algal biomass and coal.
- Full Text:
- Date Issued: 2015
- Authors: Charlie, David
- Date: 2015
- Subjects: Microalgae -- Biotechnology Biomass chemicals , Biomass energy
- Language: English
- Type: Thesis , Masters , MTech
- Identifier: http://hdl.handle.net/10948/50087 , vital:42035
- Description: In this research, the objective was to investigate under the chosen liquefaction conditions whether co-liquefaction of coal and microalgae biomass as agglomerates does possess a distinct advantage over the individual liquefaction of microalgae and coal. This was initially done by preparing dry micro algal biomass, followed by the preparation of coal fines and finally coal-algae as agglomerates. The agglomerates were prepared by continuously adsorbing wet concentrated microalgae slurry/solution onto the dry coal fines in order to prepare different ratios of coal-algae agglomerate(s). Ultimate analysis of the starting material (coal fines and the microalgae) as well the agglomerates then followed. According to research, wet microalgae biomass has the potential of acting as a hydrogen donor. By preparing the agglomerates, the rationale was to promote hydrogen supply from the micro algal biomass that has high hydrogen content to the coal with lower hydrogen content. The resulting product was then expected to have fairly higher hydrogen content. However, this was not observed or evident from the elemental analysis results. The results (elemental composition) showed no difference between the agglomerates and the coal. Based on the observed ultimate analysis results, liquefaction experiments were conducted to determine whether hydrogen transfer and the expected synergistic effect between micro algal biomass and coal would occur. The crude oil product was recovered and simulated distillation technique was used for the characterization of the crude oil product. The results we in agreement that higher yields can be achieved during co-liquefaction as compared to the individual liquefaction. A possible synergistic effect that was investigated in this research existed. Liquefaction results provided evidence of higher yields recovery from the agglomerates compared to those of coal fines and microalgae biomass. Although there was higher recovery on the agglomerates, there appears to be a downward trend (decrease) in product recovery with higher loadings of micro algal biomass on to the coal. This means that higher loading of micro algal biomass tend to suppress liquefaction of coal, thus resulting in lower product recovery. While lower loadings tend to effectively facilitate the liquefaction of coal, thus proving that the proposed synergistic effect between the two does exist. Overall product recovery (yields) of the agglomerates is higher compared to the individual recovery of coal fines and microalgae biomass. This was followed by mass balancing to determine the overall conversion. Higher conversions were achieved on the agglomerates compared to coal. This could mean that microalgae have the potential to facilitate the liquefaction of coal and improve its conversion. The observed trend results in improved conversion as well as higher oil yields (simulated distillation results). Overall, the mass balancing provided insightful information regarding the coal-microalgae interaction based on the conversion, and this further corresponds or supports the simulated distillation results. This research paper provides evidence of the synergistic effect that exists between micro algal biomass and coal.
- Full Text:
- Date Issued: 2015
Identification and documentation of ethnobiological methods used by rural farmers to control stalk borers on maize in the Eastern Cape Province of South Africa
- Authors: Skenjana, Nolitha Leonora
- Date: 2015
- Subjects: Ethnobotany , Stem borers , Biological pest control agents -- South Africa , Corn -- Diseases and pests -- South Africa
- Language: English
- Type: Thesis , Masters , MSc (Ethnobotany)
- Identifier: vital:11312 , http://hdl.handle.net/10353/d1019852 , Ethnobotany , Stem borers , Biological pest control agents -- South Africa , Corn -- Diseases and pests -- South Africa
- Description: Maize contributes substantially to food security in the Eastern Cape province of South Africa. It is a staple food to many of the province’s rural and urban inhabitants. Insect pests are one of the factors that hamper its productivity and as a result, deprive farmers of good yields. The adverse effects of insecticides and the high cost associated with them and the cost of transgenic seeds are some of the challenges faced by small-scale farmers in rural areas. Alternative control methods which include the use of indigenous techniques to control pests are now sought. A study to identify and document ethnobiological means used by rural farmers to manage insect pests of maize was conducted in the rural areas of the Eastern Cape Province. A total of 217 participants were interviewed on the matter, using semi-structured but detailed questionnaires. Rural farmers due to their linkage to agriculture activities and the fact that they are considered as custodians of agricultural indigenous knowledge were selected as respondents. Only maize producing and IsiXhosa speaking people were chosen to contribute. Main focus was on the demography of respondents, crop production activities and insect pest control. Pretesting of the questionnaire in order to assess the appropriateness of questions and comprehension by both farmers and enumerators was done. Data was analysed using descriptive statistics. Fifty five percent (55 %) of the respondents were females and the highest number of participants was from the Chris Hani District Municipality. Majority of the people were unemployed or pensioners. Most had only attended primary school and the mean age was 59 years. Apart from maize, respondents were cultivating other crops such as cabbage, Swiss chard, potatoes etc. Stalk borers followed by cutworms were the main pests of maize in these areas. Respondents used mainly insecticides, followed by alternative substances, which also included cultural control methods such as planting date manipulation. Few respondents used plants as control agents for insect pests. Some people did not control pests even though they were a problem in their fields. The most used plant was Chenopodium ambrosiodes L, while the most used substance was Madubula (a detergent). The most used insecticide was carbaryl from the carbamite family. Respondents listed different preparation techniques for all the control methods mentioned. These techniques revealed different times of preparation, quantities of ingredients, amounts applied on plants, modes of application and intervals of application. Rural farmers in the study areas used different atypical methods which may play a significant role in pest management today. Some of the products may have a positive influence on agriculture, while some are dangerous to humans and environmental health. Further research which will investigate their potential use in pest control needs to be done.
- Full Text:
- Date Issued: 2015
- Authors: Skenjana, Nolitha Leonora
- Date: 2015
- Subjects: Ethnobotany , Stem borers , Biological pest control agents -- South Africa , Corn -- Diseases and pests -- South Africa
- Language: English
- Type: Thesis , Masters , MSc (Ethnobotany)
- Identifier: vital:11312 , http://hdl.handle.net/10353/d1019852 , Ethnobotany , Stem borers , Biological pest control agents -- South Africa , Corn -- Diseases and pests -- South Africa
- Description: Maize contributes substantially to food security in the Eastern Cape province of South Africa. It is a staple food to many of the province’s rural and urban inhabitants. Insect pests are one of the factors that hamper its productivity and as a result, deprive farmers of good yields. The adverse effects of insecticides and the high cost associated with them and the cost of transgenic seeds are some of the challenges faced by small-scale farmers in rural areas. Alternative control methods which include the use of indigenous techniques to control pests are now sought. A study to identify and document ethnobiological means used by rural farmers to manage insect pests of maize was conducted in the rural areas of the Eastern Cape Province. A total of 217 participants were interviewed on the matter, using semi-structured but detailed questionnaires. Rural farmers due to their linkage to agriculture activities and the fact that they are considered as custodians of agricultural indigenous knowledge were selected as respondents. Only maize producing and IsiXhosa speaking people were chosen to contribute. Main focus was on the demography of respondents, crop production activities and insect pest control. Pretesting of the questionnaire in order to assess the appropriateness of questions and comprehension by both farmers and enumerators was done. Data was analysed using descriptive statistics. Fifty five percent (55 %) of the respondents were females and the highest number of participants was from the Chris Hani District Municipality. Majority of the people were unemployed or pensioners. Most had only attended primary school and the mean age was 59 years. Apart from maize, respondents were cultivating other crops such as cabbage, Swiss chard, potatoes etc. Stalk borers followed by cutworms were the main pests of maize in these areas. Respondents used mainly insecticides, followed by alternative substances, which also included cultural control methods such as planting date manipulation. Few respondents used plants as control agents for insect pests. Some people did not control pests even though they were a problem in their fields. The most used plant was Chenopodium ambrosiodes L, while the most used substance was Madubula (a detergent). The most used insecticide was carbaryl from the carbamite family. Respondents listed different preparation techniques for all the control methods mentioned. These techniques revealed different times of preparation, quantities of ingredients, amounts applied on plants, modes of application and intervals of application. Rural farmers in the study areas used different atypical methods which may play a significant role in pest management today. Some of the products may have a positive influence on agriculture, while some are dangerous to humans and environmental health. Further research which will investigate their potential use in pest control needs to be done.
- Full Text:
- Date Issued: 2015
Identification of agricultural and industrial pollutants in the Kat River, Eastern Cape and their effect on agricultural products found along the river banks
- Authors: Mutingwende, Nhamo
- Date: 2015
- Subjects: Environmental toxicology , Rivers -- Environmental aspects -- South Africa , Water -- Pollution -- Toxicology -- South Africa
- Language: English
- Type: Thesis , Masters , MSc (Biochemistry)
- Identifier: vital:11291 , http://hdl.handle.net/10353/d1020242 , Environmental toxicology , Rivers -- Environmental aspects -- South Africa , Water -- Pollution -- Toxicology -- South Africa
- Description: There is growing concern that commonly used Pharmaceuticals and Personal Care Products (PPCPs) and pesticides are entering and contaminating drinking water supplies. The use of targeted quantitation of PPCP has been well established but there is an emerging trend to also screen for and identify unexpected environmental pollutants. Chemicals like pesticides hormones and antibiotics are especially of interest because of proven endocrine disrupting effects and a possible development of bacterial resistance. Powerful screening methods are required to detect and quantify the presence of these compounds in our environment. PPCP encompass a wide range of pollutants, including Endocrine Disrupting Compounds (EDC), pesticides, hormones, antibiotics, drugs of abuse, x-ray contrast agents and drinking water disinfection by-products to name a few. In order to properly assess the effects of these compounds on our environment, it is necessary to accurately monitor their presence. The diversity of chemical properties of these compounds makes method development challenging. LC/MS/MS is able to analyse polar, semi-volatile, and thermally labile compounds covering a wide molecular weight range. The new AB SCIEX TripleTOF™5600 LC/MS/MS was used to profile environmental samples for unexpected pollutants, to identify and characterise the chemical composition and structure of the pollutants, and to quantify (based on intensity) the concentration in collected water samples. Liquid Chromatography coupled to tandem Mass Spectrometry (LCMS/ MS) is able to analyse polar, semi-volatile, and thermally labile compounds covering a wide molecular weight range, such as pesticides, antibiotics, drugs of abuse, x-ray contrast agents, drinking water disinfection by-products etc. More recently there is a growing interest from environmental researchers to also screen for and identify non-targeted compounds in environmental samples, including metabolites and degradates, but also completely unexpected pollutants. The new AB SCIEX TripleTOF™5600 LC/MS/MS system is capable of performing highly sensitive and fast MS scanning experiments to search for unknown molecular ions while also performing selective and characteristic MS/MS scanning for further compound identification and, therefore, is the instrument of choice for this challenging task. General unknown screening workflows do not use a target analyte list and compound detection is not based on any prior knowledge, including retention times and information on possible molecular and fragment ions. Therefore, acquired chromatograms are very rich in information and can easily contain thousands of ions from both any compounds present in the sample as well as from the sample matrix itself. Thus, powerful software tools are needed to explore such data to identify the unexpected compound. Water samples were collected both upstream and downstream of two WWTPs (Seymour and Fort Beaufort) and were directly injected on the AB SCIEX TripleTOF™5600 LC/MS/MS after being filtered. 15 sample points along the Kat River, ranging from a point as close to the source as possible to a point just before it joins the Great Fish River were used. The samples collected from the source were used as the control in each of the experiments, the assumption being the closer you get to the source, the less contaminated the water would be for the analysis of pesticides. Points were selected where the Kat River crosses the R67 or on farms where the river was accessible using farm roads. Samples were collected from October 2013 to November 2014.The Peak view software and Analyst software were used in the analysis of PPCPs. The XIC Manager allows you to manage large lists of compounds and perform automatic extracted ion chromatogram (XIC) calculations and review results operations. The results were displayed in the chromatogram pane and the XIC table (see results). The results reported here in this thesis indicate that there is contamination in the Kat River water due to both pesticides and PPCPs. The results also indicate that the food products are also contaminated and hence both the Kat River agricultural produce and its water need to be closely monitored for both pesticide and PPCPs contaminants. Further studies to investigate the quantitative levels of pesticides and PPCPs in the Kat river water to determine if the concentration levels of the detected pesticides are below the reported Maximum Residues Limits will be explored in the future.
- Full Text:
- Date Issued: 2015
- Authors: Mutingwende, Nhamo
- Date: 2015
- Subjects: Environmental toxicology , Rivers -- Environmental aspects -- South Africa , Water -- Pollution -- Toxicology -- South Africa
- Language: English
- Type: Thesis , Masters , MSc (Biochemistry)
- Identifier: vital:11291 , http://hdl.handle.net/10353/d1020242 , Environmental toxicology , Rivers -- Environmental aspects -- South Africa , Water -- Pollution -- Toxicology -- South Africa
- Description: There is growing concern that commonly used Pharmaceuticals and Personal Care Products (PPCPs) and pesticides are entering and contaminating drinking water supplies. The use of targeted quantitation of PPCP has been well established but there is an emerging trend to also screen for and identify unexpected environmental pollutants. Chemicals like pesticides hormones and antibiotics are especially of interest because of proven endocrine disrupting effects and a possible development of bacterial resistance. Powerful screening methods are required to detect and quantify the presence of these compounds in our environment. PPCP encompass a wide range of pollutants, including Endocrine Disrupting Compounds (EDC), pesticides, hormones, antibiotics, drugs of abuse, x-ray contrast agents and drinking water disinfection by-products to name a few. In order to properly assess the effects of these compounds on our environment, it is necessary to accurately monitor their presence. The diversity of chemical properties of these compounds makes method development challenging. LC/MS/MS is able to analyse polar, semi-volatile, and thermally labile compounds covering a wide molecular weight range. The new AB SCIEX TripleTOF™5600 LC/MS/MS was used to profile environmental samples for unexpected pollutants, to identify and characterise the chemical composition and structure of the pollutants, and to quantify (based on intensity) the concentration in collected water samples. Liquid Chromatography coupled to tandem Mass Spectrometry (LCMS/ MS) is able to analyse polar, semi-volatile, and thermally labile compounds covering a wide molecular weight range, such as pesticides, antibiotics, drugs of abuse, x-ray contrast agents, drinking water disinfection by-products etc. More recently there is a growing interest from environmental researchers to also screen for and identify non-targeted compounds in environmental samples, including metabolites and degradates, but also completely unexpected pollutants. The new AB SCIEX TripleTOF™5600 LC/MS/MS system is capable of performing highly sensitive and fast MS scanning experiments to search for unknown molecular ions while also performing selective and characteristic MS/MS scanning for further compound identification and, therefore, is the instrument of choice for this challenging task. General unknown screening workflows do not use a target analyte list and compound detection is not based on any prior knowledge, including retention times and information on possible molecular and fragment ions. Therefore, acquired chromatograms are very rich in information and can easily contain thousands of ions from both any compounds present in the sample as well as from the sample matrix itself. Thus, powerful software tools are needed to explore such data to identify the unexpected compound. Water samples were collected both upstream and downstream of two WWTPs (Seymour and Fort Beaufort) and were directly injected on the AB SCIEX TripleTOF™5600 LC/MS/MS after being filtered. 15 sample points along the Kat River, ranging from a point as close to the source as possible to a point just before it joins the Great Fish River were used. The samples collected from the source were used as the control in each of the experiments, the assumption being the closer you get to the source, the less contaminated the water would be for the analysis of pesticides. Points were selected where the Kat River crosses the R67 or on farms where the river was accessible using farm roads. Samples were collected from October 2013 to November 2014.The Peak view software and Analyst software were used in the analysis of PPCPs. The XIC Manager allows you to manage large lists of compounds and perform automatic extracted ion chromatogram (XIC) calculations and review results operations. The results were displayed in the chromatogram pane and the XIC table (see results). The results reported here in this thesis indicate that there is contamination in the Kat River water due to both pesticides and PPCPs. The results also indicate that the food products are also contaminated and hence both the Kat River agricultural produce and its water need to be closely monitored for both pesticide and PPCPs contaminants. Further studies to investigate the quantitative levels of pesticides and PPCPs in the Kat river water to determine if the concentration levels of the detected pesticides are below the reported Maximum Residues Limits will be explored in the future.
- Full Text:
- Date Issued: 2015
Identification of novel SNPSTRs by 454 sequencing in Nguni and Sotho-Tswana populations
- Authors: Laurence, Jo-Anne Elizabeth
- Date: 2015
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/55885 , vital:26752
- Description: DNA profiling is currently performed by analysis of the electropherogram that results following the amplification of a panel of Short Tandem Repeat (STR) loci. A need has arisen, however, for the development of a typing method that generates results which are compatible and comparable with existing databases, but that have a higher discrimination power by supplying sequence data as well as repeat-number data. Recent studies that explore these alternative typing methodologies have revealed the existence of a number of STR variants. There is, however, little information about the exact nature and prevalence of these sub-alleles. There have also been limited population studies of the genetic profiles of sub-Saharan African populations, despite the fact that evidence suggests that there is greater genetic structure and genetic diversity in these populations. In this study, a processing protocol for the generation of 454 sequencing-ready amplicons of vWA, D2S441, D3S1358, D13S317, D21S11 and D7S820 loci was developed. This protocol was applied to buccal swabs collected from 144 individuals of the Nguni and Sotho-Tswana population groups. A total of 145 485 reads were obtained from the sequencing of these amplicons, of which 97 400 and 48 085 reads were obtained for the Nguni and Sotho-Tswana populations respectively. The proportional representation for each locus ranged from 8-20%, and the allele calls and observed frequencies of these alleles suggested a high degree of relatedness between population groups. The sequencing results, furthermore, enabled the identification of a number of previously undescribed STR variants and SNPSTRs; with allele 13´ for D13S317 representing a SNP that may be predictive of Nguni-ancestry. The results also demonstrated the usefulness of next generation sequencing for increasing the number of discernible alleles for STR profiling.
- Full Text:
- Date Issued: 2015
- Authors: Laurence, Jo-Anne Elizabeth
- Date: 2015
- Language: English
- Type: text , Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/55885 , vital:26752
- Description: DNA profiling is currently performed by analysis of the electropherogram that results following the amplification of a panel of Short Tandem Repeat (STR) loci. A need has arisen, however, for the development of a typing method that generates results which are compatible and comparable with existing databases, but that have a higher discrimination power by supplying sequence data as well as repeat-number data. Recent studies that explore these alternative typing methodologies have revealed the existence of a number of STR variants. There is, however, little information about the exact nature and prevalence of these sub-alleles. There have also been limited population studies of the genetic profiles of sub-Saharan African populations, despite the fact that evidence suggests that there is greater genetic structure and genetic diversity in these populations. In this study, a processing protocol for the generation of 454 sequencing-ready amplicons of vWA, D2S441, D3S1358, D13S317, D21S11 and D7S820 loci was developed. This protocol was applied to buccal swabs collected from 144 individuals of the Nguni and Sotho-Tswana population groups. A total of 145 485 reads were obtained from the sequencing of these amplicons, of which 97 400 and 48 085 reads were obtained for the Nguni and Sotho-Tswana populations respectively. The proportional representation for each locus ranged from 8-20%, and the allele calls and observed frequencies of these alleles suggested a high degree of relatedness between population groups. The sequencing results, furthermore, enabled the identification of a number of previously undescribed STR variants and SNPSTRs; with allele 13´ for D13S317 representing a SNP that may be predictive of Nguni-ancestry. The results also demonstrated the usefulness of next generation sequencing for increasing the number of discernible alleles for STR profiling.
- Full Text:
- Date Issued: 2015
Identity in the media in a post-apartheid radio station in South Africa: the case of Lotus FM
- Authors: Pillay, Divinia
- Date: 2015
- Subjects: Public broadcasting -- Political aspects -- South Africa , Radio stations -- South Africa , Post-apartheid era -- South Africa , Identity politics -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10948/5709 , vital:20971
- Description: This research study investigates Lotus FM, as one of many South African Media components that are catering for one specific cultural or religious group. The investigation explores the implications of practice of a pecific media component that caters for specific cultural or religious groups operating in a post-apartheid South Africa. After the end of the apartheid era in South Africa, a number of South African media components have proclaimed their commitment to reconciliation and nation building within South Africa by attempting to unite audiences. The South African Broadcasting Corporation, which held the monopoly on South African Broadcasting for decades, has promulgated the notion of the rainbow nation to audiences in South Africa. Since 1994, sub-components of the different South African media segments were developed to cater for specific ethnic or cultural groups by the station managements. This was aimed at reversing the effects of pre-1994 media that catered for the former ruling minority only or ethnic groups that were categorized by the former political dispensation. It is possible, however, that this has resulted in a renewed and continued separation of interest groups present in South Africa today.
- Full Text:
- Date Issued: 2015
- Authors: Pillay, Divinia
- Date: 2015
- Subjects: Public broadcasting -- Political aspects -- South Africa , Radio stations -- South Africa , Post-apartheid era -- South Africa , Identity politics -- South Africa
- Language: English
- Type: Thesis , Masters , MA
- Identifier: http://hdl.handle.net/10948/5709 , vital:20971
- Description: This research study investigates Lotus FM, as one of many South African Media components that are catering for one specific cultural or religious group. The investigation explores the implications of practice of a pecific media component that caters for specific cultural or religious groups operating in a post-apartheid South Africa. After the end of the apartheid era in South Africa, a number of South African media components have proclaimed their commitment to reconciliation and nation building within South Africa by attempting to unite audiences. The South African Broadcasting Corporation, which held the monopoly on South African Broadcasting for decades, has promulgated the notion of the rainbow nation to audiences in South Africa. Since 1994, sub-components of the different South African media segments were developed to cater for specific ethnic or cultural groups by the station managements. This was aimed at reversing the effects of pre-1994 media that catered for the former ruling minority only or ethnic groups that were categorized by the former political dispensation. It is possible, however, that this has resulted in a renewed and continued separation of interest groups present in South Africa today.
- Full Text:
- Date Issued: 2015
If I Stay Right Here
- Authors: Ngamlana, Chwayita
- Date: 2015
- Subjects: Uncatalogued
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/193498 , vital:45337
- Description: My novella concerns the dynamics of a relationship between two girls. It shows the heterosexual‐like nature of the relationship rather than dwelling on the pressures on lesbians from society as a whole. At its core is the raw emotion and passion of the relationship, which is at the same time toxic, destructive and volatile because of their class differences and other insecurities. The work is influenced by the grit, openness, and innovation of several contemporary writers. , Thesis (MA) -- Faculty of Humanities, Institute for the Study of English in Africa, 2015
- Full Text:
- Date Issued: 2015
- Authors: Ngamlana, Chwayita
- Date: 2015
- Subjects: Uncatalogued
- Language: English
- Type: Master's theses , text
- Identifier: http://hdl.handle.net/10962/193498 , vital:45337
- Description: My novella concerns the dynamics of a relationship between two girls. It shows the heterosexual‐like nature of the relationship rather than dwelling on the pressures on lesbians from society as a whole. At its core is the raw emotion and passion of the relationship, which is at the same time toxic, destructive and volatile because of their class differences and other insecurities. The work is influenced by the grit, openness, and innovation of several contemporary writers. , Thesis (MA) -- Faculty of Humanities, Institute for the Study of English in Africa, 2015
- Full Text:
- Date Issued: 2015
IFLA WLIC 2015 Volunteers/Delegates feedback session 8 September 2015, Rhodes University Library
- Date: 2015
- Language: English
- Type: Text
- Identifier: vital:7958 , http://hdl.handle.net/10962/d1019838
- Description: IFLA WLIC 2015 feedback session by Rhodes University Library Staff who attended the Congress as Volunteers and Delegates, Grahamstown, 8 September 2015.
- Full Text:
- Date Issued: 2015
- Date: 2015
- Language: English
- Type: Text
- Identifier: vital:7958 , http://hdl.handle.net/10962/d1019838
- Description: IFLA WLIC 2015 feedback session by Rhodes University Library Staff who attended the Congress as Volunteers and Delegates, Grahamstown, 8 September 2015.
- Full Text:
- Date Issued: 2015
Igqirha linxiba iimfele zokuqala 01
- Unnamed
- Authors: Unnamed
- Date: 2015
- Subjects: UNCATALOGUED
- Type: Video
- Identifier: http://hdl.handle.net/10962/303719 , vital:58365 , KHV-274
- Description: Traditional Healers dancing and singing in a circle , Use of this resource is governed by the terms and conditions of the Creative Commons "Attribution-NonCommercial-ShareAlike" License (http://creativecommons.org/licenses/by-nc-sa/2.0/) (NEVER CAHNGE THIS FIELD)
- Full Text: false
- Date Issued: 2015
- Authors: Unnamed
- Date: 2015
- Subjects: UNCATALOGUED
- Type: Video
- Identifier: http://hdl.handle.net/10962/303719 , vital:58365 , KHV-274
- Description: Traditional Healers dancing and singing in a circle , Use of this resource is governed by the terms and conditions of the Creative Commons "Attribution-NonCommercial-ShareAlike" License (http://creativecommons.org/licenses/by-nc-sa/2.0/) (NEVER CAHNGE THIS FIELD)
- Full Text: false
- Date Issued: 2015