Genetic and biological characterisation of a novel South African Cydia pomonella granulovirus (CpGV-SA) isolate
- Motsoeneng, Boitumelo Madika
- Authors: Motsoeneng, Boitumelo Madika
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:20503 , http://hdl.handle.net/10962/d1021266
- Description: The codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae), is the primary pest of pome fruit cultivated worldwide. The control of this insect pest has been dependent on the frequent use of broad-spectrum chemical pesticides, which has led to the development of resistance in pest populations and negative effects on human health and the environment. The Betabaculovirus of C. pomonella has successfully been applied as a biological control agent in integrated pest management (IPM) programmes for the suppression of pest populations worldwide. Previously, all Cydia pomonella granulovirus (CpGV) biopesticides were based on a Mexican isolate (CpGV-M) and although these products are highly efficient at controlling C. pomonella, resistance cases have been reported across Europe. The identification of novel CpGV isolates as additional or alternative control agents to manage resistance is therefore necessary. This study aimed to genetically and biologically characterise a novel South African C. pomonella granulovirus isolate and to test its virulence against neonate larvae. Based on the morphology of the occlusion bodies observed using transmission electron microscopy, granuloviruses were recovered from diseased and dead larvae collected from an orchard in South Africa where no virus applications had been made. DNA was extracted and the identification of the isolated granulovirus was achieved through the PCR amplification and sequencing of the lef-8, lef-9, granulin and egt genes. Submission of the gene sequences to BLAST revealed high percentage identities to sequences from various CpGV isolates, resulting in the naming of the isolate in this study as the South African Cydia pomonella granulovirus (CpGV-SA) isolate. Phylogenetic analysis based on the single nucleotide polymorphisms (SNPs) detected in the lef-8, lef-9 and granulin nucleotide sequences grouped the South African isolate with CpGV-E2 (genome type B) and CpGV-S (genome type E). The CpGV-SA isolate was further genetically characterised by restriction endonuclease analysis and complete sequencing of the genomic DNA. Differences were observed for the BamHI, EcoRI, PstI and XhoI profiles of CpGV-SA in comparison to the respective profiles generated for CpGV-M extracted from a biopesticide, Carpovirusine® (Arysta Lifescience, France). Several genetic variations between the complete genome sequence of CpGV-SA and the reference isolate, CpGV-M1, as well as a recent genome submission of CpGV-M, both representing genome type A were observed. The complete genome analysis confirmed that CpGV-SA is genetically different from the Mexican CpGV isolate, used in thedevelopment of most biopesticides. In silico restriction profiles of the genome sequence obtained for CpGV-SA and genome sequences of genetically different CpGV isolates originating from Mexico (M1 and M), England (E2), Canada (S) and Iran (I12 and I07), available on the NCBI’s GenBank database confirmed that CpGV-SA is of mixed genotypes. Furthermore, the South African isolate shared the single common difference found in the pe38 gene of resistance overcoming isolates, which was the absence of an internal 24 nucleotide repeat present in CpGV-M1. In addition to the common difference, SNPs detected in the pe38 gene grouped the isolate with the CpGV-S isolate, suggesting that the CpGV-SA isolate is predominantly of genome type E. To determine the biological activity of CpGV-SA against neonate C. pomonella larvae, surface bioassays were conducted alongside CpGV-M (Carpovirusine®) bioassays. The LC50 and LC90 values for the South African isolate were 1.6 × 103 and 1.2 × 105 OBs/ml respectively. The LT50 was determined to be 135 hours. These values were similar to the values obtained for CpGV-M (Carpovirusine®). The results in this study suggest that a novel South African CpGV isolate of mixed genotypes, potentially able to overcome resistance in C. pomonella, with biological activity similar to CpGV-M (Carpovirusine®) and important for the control of C. pomonella was recovered. The CpGV-SA isolate could therefore potentially be developed into a biopesticide for use in resistance management strategies against C. pomonella populations in South Africa.
- Full Text:
- Authors: Motsoeneng, Boitumelo Madika
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: vital:20503 , http://hdl.handle.net/10962/d1021266
- Description: The codling moth, Cydia pomonella (L.) (Lepidoptera: Tortricidae), is the primary pest of pome fruit cultivated worldwide. The control of this insect pest has been dependent on the frequent use of broad-spectrum chemical pesticides, which has led to the development of resistance in pest populations and negative effects on human health and the environment. The Betabaculovirus of C. pomonella has successfully been applied as a biological control agent in integrated pest management (IPM) programmes for the suppression of pest populations worldwide. Previously, all Cydia pomonella granulovirus (CpGV) biopesticides were based on a Mexican isolate (CpGV-M) and although these products are highly efficient at controlling C. pomonella, resistance cases have been reported across Europe. The identification of novel CpGV isolates as additional or alternative control agents to manage resistance is therefore necessary. This study aimed to genetically and biologically characterise a novel South African C. pomonella granulovirus isolate and to test its virulence against neonate larvae. Based on the morphology of the occlusion bodies observed using transmission electron microscopy, granuloviruses were recovered from diseased and dead larvae collected from an orchard in South Africa where no virus applications had been made. DNA was extracted and the identification of the isolated granulovirus was achieved through the PCR amplification and sequencing of the lef-8, lef-9, granulin and egt genes. Submission of the gene sequences to BLAST revealed high percentage identities to sequences from various CpGV isolates, resulting in the naming of the isolate in this study as the South African Cydia pomonella granulovirus (CpGV-SA) isolate. Phylogenetic analysis based on the single nucleotide polymorphisms (SNPs) detected in the lef-8, lef-9 and granulin nucleotide sequences grouped the South African isolate with CpGV-E2 (genome type B) and CpGV-S (genome type E). The CpGV-SA isolate was further genetically characterised by restriction endonuclease analysis and complete sequencing of the genomic DNA. Differences were observed for the BamHI, EcoRI, PstI and XhoI profiles of CpGV-SA in comparison to the respective profiles generated for CpGV-M extracted from a biopesticide, Carpovirusine® (Arysta Lifescience, France). Several genetic variations between the complete genome sequence of CpGV-SA and the reference isolate, CpGV-M1, as well as a recent genome submission of CpGV-M, both representing genome type A were observed. The complete genome analysis confirmed that CpGV-SA is genetically different from the Mexican CpGV isolate, used in thedevelopment of most biopesticides. In silico restriction profiles of the genome sequence obtained for CpGV-SA and genome sequences of genetically different CpGV isolates originating from Mexico (M1 and M), England (E2), Canada (S) and Iran (I12 and I07), available on the NCBI’s GenBank database confirmed that CpGV-SA is of mixed genotypes. Furthermore, the South African isolate shared the single common difference found in the pe38 gene of resistance overcoming isolates, which was the absence of an internal 24 nucleotide repeat present in CpGV-M1. In addition to the common difference, SNPs detected in the pe38 gene grouped the isolate with the CpGV-S isolate, suggesting that the CpGV-SA isolate is predominantly of genome type E. To determine the biological activity of CpGV-SA against neonate C. pomonella larvae, surface bioassays were conducted alongside CpGV-M (Carpovirusine®) bioassays. The LC50 and LC90 values for the South African isolate were 1.6 × 103 and 1.2 × 105 OBs/ml respectively. The LT50 was determined to be 135 hours. These values were similar to the values obtained for CpGV-M (Carpovirusine®). The results in this study suggest that a novel South African CpGV isolate of mixed genotypes, potentially able to overcome resistance in C. pomonella, with biological activity similar to CpGV-M (Carpovirusine®) and important for the control of C. pomonella was recovered. The CpGV-SA isolate could therefore potentially be developed into a biopesticide for use in resistance management strategies against C. pomonella populations in South Africa.
- Full Text:
Genetic structure and biogeography of three wrasse species (Labridae) within the Western Indian Ocean
- Authors: Mayekiso, Sisanda
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/1114 , vital:20021
- Description: The biogeography of wrasses within the Western Indian Ocean (WIO) is poorly understood, with regard to origins and genetic differentiation or connectivity among the regions of the WIO. This region is a good model for studying the influence of physical complexities and biogeographic breaks in shaping patterns of differentiation in wrasses. Three reef-associated fish species, Cheilio inermis, Thalassoma hebraicum and T. lunare, were selected to examine the factors that have influenced patterns of differentiation across the WIO. Each species was sampled from various localities of the WIO, the Red Sea and Indo-West Pacific Islands. Sequence data were generated from two mitochondrial gene fragments (cytochrome b and ATPase 6) and one nuclear locus (the first intron of the ribosomal protein S7 gene). Genetic analyses were used to calculate genetic diversity indices within species, which were then compared among species. The relationships among haplotypes and alleles were constructed using median-joining networks. Where necessary, neighbour-joining trees (NJ) were constructed to examine relationships among haplotypes and alleles for the Thalassoma species. Population structure was analysed using AMOVA and pairwise ФST to compare and calculate differentiation between the WIO localities. Mismatch distributions were used to examine population growth and decline or stability, and demographic parameters were used to calculate time of population expansion. There was high haplotype (h = 0.88 to 0.98) and low nucleotide diversities (π = 0.003 to 0.008) among all species for mitochondrial markers. For S7 intron I, high allelic (A = 0.95 to 0.98) and low nucleotide diversities (π = 0.002 to 0.014) were observed for all species. The pairwise ФST values revealed little to great (ФST = -0.02 to 0.67) genetic differentiation between localities, across all species for the three gene regions. The pairwise comparisons indicated the differentiation in C. inermis of Tanzania and Kenya from Mozambique and Nosy Be (Madagascar). The widespread C. inermis also revealed the differentiation of Kenya and Tanzania. For C. inermis, the AMOVAs of ATPase 6 and cytochrome b data indicated high differentiation among defined locality groups. The groups were defined according to geographic proximity. However, the AMOVA of the nuclear gene (S7 intron I) did not find variation among defined locality groups. Cheilio inermis revealed a sequence divergence of 0.4%. The divergence that was found in C. inermis was not enough to suggest a cryptic species within the WIO. Overall, the widespread and monotypic C. inermis revealed genetic differentiation within the WIO. Thalassoma hebraicum generally revealed little genetic differentiation across the WIO. The AMOVAs of the three gene regions showed no variation among specimens of the defined locality groups. However, some differentiation was found between localities. The pairwise comparisons of T. hebraicum revealed the differentiation of Seychelles from the African mainland and Madagascar. Southern Africa was observed to be differentiated from Nosy Be and Zanzibar. The observed differentiation could be caused by oceanic barriers such as the South Equatorial Current (SEQC), East African Coastal Current (EACC), and the Comoros Gyres and eddies in the Mozambique Channel, and Agulhas Current. Thalassoma lunare revealed genetic isolation between the WIO and the Red Sea as well as within the WIO. The genetic isolation between the WIO and the Red Sea is probably due to the historical isolation by the Bab al Mandab and contemporary barriers such as the cold upwelling cells in Somalia. The differentiation of Maldives from the African mainland and Seychelles could be due to distance and the upwelling cells created by monsoon winds. Mismatch distributions suggested that C. inermis and T. hebraicum had undergone demographic expansion during the Pleistocene (92 678 to 40 219 years ago). The results of the current study are similar to those from previous studies of WIO reef fish species, and the results of the present study could have potential implications for conservation and fisheries management. Single genetic markers and single species studies do not detect all barriers to dispersal in the WIO, thus they are insufficient to inform conservation management. Thus, the use of multispecies and genetic markers in the current study can be adopted by other studies of the marine taxa of the WIO.
- Full Text:
- Authors: Mayekiso, Sisanda
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/1114 , vital:20021
- Description: The biogeography of wrasses within the Western Indian Ocean (WIO) is poorly understood, with regard to origins and genetic differentiation or connectivity among the regions of the WIO. This region is a good model for studying the influence of physical complexities and biogeographic breaks in shaping patterns of differentiation in wrasses. Three reef-associated fish species, Cheilio inermis, Thalassoma hebraicum and T. lunare, were selected to examine the factors that have influenced patterns of differentiation across the WIO. Each species was sampled from various localities of the WIO, the Red Sea and Indo-West Pacific Islands. Sequence data were generated from two mitochondrial gene fragments (cytochrome b and ATPase 6) and one nuclear locus (the first intron of the ribosomal protein S7 gene). Genetic analyses were used to calculate genetic diversity indices within species, which were then compared among species. The relationships among haplotypes and alleles were constructed using median-joining networks. Where necessary, neighbour-joining trees (NJ) were constructed to examine relationships among haplotypes and alleles for the Thalassoma species. Population structure was analysed using AMOVA and pairwise ФST to compare and calculate differentiation between the WIO localities. Mismatch distributions were used to examine population growth and decline or stability, and demographic parameters were used to calculate time of population expansion. There was high haplotype (h = 0.88 to 0.98) and low nucleotide diversities (π = 0.003 to 0.008) among all species for mitochondrial markers. For S7 intron I, high allelic (A = 0.95 to 0.98) and low nucleotide diversities (π = 0.002 to 0.014) were observed for all species. The pairwise ФST values revealed little to great (ФST = -0.02 to 0.67) genetic differentiation between localities, across all species for the three gene regions. The pairwise comparisons indicated the differentiation in C. inermis of Tanzania and Kenya from Mozambique and Nosy Be (Madagascar). The widespread C. inermis also revealed the differentiation of Kenya and Tanzania. For C. inermis, the AMOVAs of ATPase 6 and cytochrome b data indicated high differentiation among defined locality groups. The groups were defined according to geographic proximity. However, the AMOVA of the nuclear gene (S7 intron I) did not find variation among defined locality groups. Cheilio inermis revealed a sequence divergence of 0.4%. The divergence that was found in C. inermis was not enough to suggest a cryptic species within the WIO. Overall, the widespread and monotypic C. inermis revealed genetic differentiation within the WIO. Thalassoma hebraicum generally revealed little genetic differentiation across the WIO. The AMOVAs of the three gene regions showed no variation among specimens of the defined locality groups. However, some differentiation was found between localities. The pairwise comparisons of T. hebraicum revealed the differentiation of Seychelles from the African mainland and Madagascar. Southern Africa was observed to be differentiated from Nosy Be and Zanzibar. The observed differentiation could be caused by oceanic barriers such as the South Equatorial Current (SEQC), East African Coastal Current (EACC), and the Comoros Gyres and eddies in the Mozambique Channel, and Agulhas Current. Thalassoma lunare revealed genetic isolation between the WIO and the Red Sea as well as within the WIO. The genetic isolation between the WIO and the Red Sea is probably due to the historical isolation by the Bab al Mandab and contemporary barriers such as the cold upwelling cells in Somalia. The differentiation of Maldives from the African mainland and Seychelles could be due to distance and the upwelling cells created by monsoon winds. Mismatch distributions suggested that C. inermis and T. hebraicum had undergone demographic expansion during the Pleistocene (92 678 to 40 219 years ago). The results of the current study are similar to those from previous studies of WIO reef fish species, and the results of the present study could have potential implications for conservation and fisheries management. Single genetic markers and single species studies do not detect all barriers to dispersal in the WIO, thus they are insufficient to inform conservation management. Thus, the use of multispecies and genetic markers in the current study can be adopted by other studies of the marine taxa of the WIO.
- Full Text:
Geochemical exploration for copper - cobalt in the Democratic Republic of Congo, Central African Copperbelt: a case study on PR851
- Authors: Katombe-Kisumbule, Paul
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/3035 , vital:20354
- Description: The PR851 licence area is located at about 80 km west from the town of Likasi in the district of Haut-Katanga and 175 km north-west of Lubumbashi, the capital city of Katanga Province in the Democratic Republic of Congo. The exploration licence was granted by the government of the Democratic Republic of Congo to First Quantum Minerals Ltd through its Congolese subsidiary Compagnie Minière de Sakania Sprl (CoMiSa Sprl) under certificate N˚ CAMI/CR/70/2003 on the 10th of October 2003 for a period of 5 years with a possibility of renewal for 3 years in respect to the new Congolese mining code. The PR851 area lies on fragments of Mines Subgroup rocks of the Roan Group in the Congolese Copperbelt where most of the Cu-Co and stratiform-stratabound deposits such as Kipushi, Ruashi-Etoile, Kinsevere, Kipoi, Luishya, Luswishi, Shituru, Kamoya, Kambove, Tenke- Fungurume, Shinkolobwe, Swambo, Mindingi and Kamoto among others are found. During the 20th century, the Union Minière du Haut Katanga (U.M.H.K.) undertook mineral exploration in the Congolese Copperbelt and numerous copper- and cobalt-occurrences were identified (for instance Kibamba copper occurrence in PR851 area). From 2003, the Compagnie Minière de Sakania Sprl initiated a grassroots exploration program in PR851 area and geochemical exploration survey as one of the mineral exploration tools was implemented to aim at detecting copper and cobalt concentration in soil. The B horizon of the thick tropical soil in the area was sampled and soil samples were sent to Genalysis laboratories in Johannesburg, Republic of South Africa for main chemical analysis of Cu and Co only, whereas 10% of analyzed samples were dispatched to Perth, Western Australia for quality control analysis. Thresholds for anomalies of copper and cobalt were defined by literature comparison, standard deviations and spatial analysis. The anomalies were tested at a later stage by reverse circulation / diamond drilling during the year of 2005 to 2008 and the Cu-Co resources were estimated by Digital Mining Services of Harare, Zimbabwe in the year of 2008. Geological logging of chips from reverse circulation and diamond drill cores revealed that copper mineralization is represented by malachite, chrysocolla, chalcopyrite and bornite whereas cobalt mineralization appeared in form of heterogenite. The source of supergene mineralization remains unknown. Recommendations have been made to undertake more geological exploration work in order to fully investigate the geological setting and structural architecture of the region, which may result in a better understanding of the Cu-Co mineralization system and ore genesis. The latter has been no consensus up-to-date and different theories have been proposed to discuss the ore genesis, including syn- and dia- genetic, synorogenic and sulphide remobilization to late-to-post- orogenic Cu-Zn-Pb Kipushi-type deposit. However, geological observations favored that the diagenetic and syngenetic models are applicable to numerous deposits in the Central African Copperbelt.
- Full Text:
- Authors: Katombe-Kisumbule, Paul
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/3035 , vital:20354
- Description: The PR851 licence area is located at about 80 km west from the town of Likasi in the district of Haut-Katanga and 175 km north-west of Lubumbashi, the capital city of Katanga Province in the Democratic Republic of Congo. The exploration licence was granted by the government of the Democratic Republic of Congo to First Quantum Minerals Ltd through its Congolese subsidiary Compagnie Minière de Sakania Sprl (CoMiSa Sprl) under certificate N˚ CAMI/CR/70/2003 on the 10th of October 2003 for a period of 5 years with a possibility of renewal for 3 years in respect to the new Congolese mining code. The PR851 area lies on fragments of Mines Subgroup rocks of the Roan Group in the Congolese Copperbelt where most of the Cu-Co and stratiform-stratabound deposits such as Kipushi, Ruashi-Etoile, Kinsevere, Kipoi, Luishya, Luswishi, Shituru, Kamoya, Kambove, Tenke- Fungurume, Shinkolobwe, Swambo, Mindingi and Kamoto among others are found. During the 20th century, the Union Minière du Haut Katanga (U.M.H.K.) undertook mineral exploration in the Congolese Copperbelt and numerous copper- and cobalt-occurrences were identified (for instance Kibamba copper occurrence in PR851 area). From 2003, the Compagnie Minière de Sakania Sprl initiated a grassroots exploration program in PR851 area and geochemical exploration survey as one of the mineral exploration tools was implemented to aim at detecting copper and cobalt concentration in soil. The B horizon of the thick tropical soil in the area was sampled and soil samples were sent to Genalysis laboratories in Johannesburg, Republic of South Africa for main chemical analysis of Cu and Co only, whereas 10% of analyzed samples were dispatched to Perth, Western Australia for quality control analysis. Thresholds for anomalies of copper and cobalt were defined by literature comparison, standard deviations and spatial analysis. The anomalies were tested at a later stage by reverse circulation / diamond drilling during the year of 2005 to 2008 and the Cu-Co resources were estimated by Digital Mining Services of Harare, Zimbabwe in the year of 2008. Geological logging of chips from reverse circulation and diamond drill cores revealed that copper mineralization is represented by malachite, chrysocolla, chalcopyrite and bornite whereas cobalt mineralization appeared in form of heterogenite. The source of supergene mineralization remains unknown. Recommendations have been made to undertake more geological exploration work in order to fully investigate the geological setting and structural architecture of the region, which may result in a better understanding of the Cu-Co mineralization system and ore genesis. The latter has been no consensus up-to-date and different theories have been proposed to discuss the ore genesis, including syn- and dia- genetic, synorogenic and sulphide remobilization to late-to-post- orogenic Cu-Zn-Pb Kipushi-type deposit. However, geological observations favored that the diagenetic and syngenetic models are applicable to numerous deposits in the Central African Copperbelt.
- Full Text:
Geology, regional diamond exploration and diamond provenance of the proterozoic diamondiferous Umkondo conglomerates, Umkondo group, eastern Zimbabwe
- Authors: Zhou, Takawira
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/3598 , vital:20528
- Description: The Umkondo Sedimentary Basin in eastern Zimbabwe has been studied by various individuals and organizations since 1901. Their interest had been in finding limestone and beryl and base metal deposits, especially copper, iron and uranium, but these occurrences had proved to be of no economic value (Watson, 1969). The recent discovery of placer diamonds within the Proterozoic basal conglomerates of the Umkondo Sedimentary Basin has now attracted worldwide interest in the basin’s diamond economic potential, in understanding of the geology, and the diamond provenance of the Umkondo conglomerates. The Umkondo Sedimentary Basin basal conglomerate placer deposit might narrowly be defined as a mega-placer because of its sheer large size and high grades, especially on the 70,000 hectare western margin diamond dispersion halo where alluvial diamonds are being mined. Bluck, et al., (2005, pp 213) defines a diamond mega-placer as: . . . a number of linked deposits that are a result of one or a continuous process of transportation and deposition and holds or have held at least >= 50 million carats at >= 95% gem quality, for example, the Orange-Vaal dispersal, off the Kaapvaal craton in South Africa. On craton placers are residual, and transient placers are eroded and deposited into the exit drainage, while terminal placers, the final depositories of diamonds with the highest chances of being mega-placers are deposited into terminal basins like oceans and foreland basins. Though data is limited at the moment, the Umkondo conglomerates caratage is likely to run into hundreds of millions of carats, with a diamond gem content of between twenty and twenty-five percent, as is indicated from recent diamond production data. The greater part of the Umkondo diamonds are likely to be lodged beneath the deep gravels of the Middle and Lower Save River basin, because small remnant tilted mountain blocks and inselbergs forming the caps of hills are what remains of the host conglomerate on the western margin of the Umkondo Basin. Areas to be examined in this document will be the geology; the mode of formation of the Umkondo basin and its sedimentary system; the regional kimberlite exploration around the basin; and diamond production in the Marange diamond field, in order to come up with indications of the provenance of the diamonds within the Umkondo conglomerates. The kimberlite clusters in and around the Umkondo sedimentary basin have all proved to be barren or only nominally diamondiferous and that the kimberlites are between 200Ma and 500Ma and thus much younger than the greater than 1.1Ga Umkondo diamondiferous conglomerates. Studies so far undertaken have not managed to point to the origin, or provenance, of the Umkondo or Marange diamonds, which were discovered on the western edge of the Umkondo Basin and in the east of the basin below the Chimanimani Mountains along the Haroni River. This paper is an attempt to clear up some of the misconceptions surrounding the Marange diamond deposit and to raise interest in the urgent rquirement to study and understand the Umkondo Basin and the origin of its diamonds. The only meaningful studies on diamond occurrence and diamond exploration of the basin were undertaken from 1996 to 2006 by Kimberlitic Searches Zimbabwe (Pvt) Ltd, the then Zimbabwe kimberlite exploration arm of De Beers, Zimbabwe, in their quest to find kimberlites, which were thought to be related to the Umkondo alluvial diamond deposit. As will be shown in the following chapters, many of the discovered kimberlites range from very low grade to non-diamondiferous, and are much younger than the Umkondo conglomerates, whose diamonds are in turn a great deal older. Thus the basic question concerning the origin or provenance of the Umkondo placer diamonds still remains unresolved. Because of the sheer size of the basin, modern, wide-area-coverage, geophysical exploration methods become appropriate to effectively generate diamond potential targets for further examination. This document will attempt to collate various data available to paint a true picture of the state of exploration within the Umkondo Basin.
- Full Text:
- Authors: Zhou, Takawira
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/3598 , vital:20528
- Description: The Umkondo Sedimentary Basin in eastern Zimbabwe has been studied by various individuals and organizations since 1901. Their interest had been in finding limestone and beryl and base metal deposits, especially copper, iron and uranium, but these occurrences had proved to be of no economic value (Watson, 1969). The recent discovery of placer diamonds within the Proterozoic basal conglomerates of the Umkondo Sedimentary Basin has now attracted worldwide interest in the basin’s diamond economic potential, in understanding of the geology, and the diamond provenance of the Umkondo conglomerates. The Umkondo Sedimentary Basin basal conglomerate placer deposit might narrowly be defined as a mega-placer because of its sheer large size and high grades, especially on the 70,000 hectare western margin diamond dispersion halo where alluvial diamonds are being mined. Bluck, et al., (2005, pp 213) defines a diamond mega-placer as: . . . a number of linked deposits that are a result of one or a continuous process of transportation and deposition and holds or have held at least >= 50 million carats at >= 95% gem quality, for example, the Orange-Vaal dispersal, off the Kaapvaal craton in South Africa. On craton placers are residual, and transient placers are eroded and deposited into the exit drainage, while terminal placers, the final depositories of diamonds with the highest chances of being mega-placers are deposited into terminal basins like oceans and foreland basins. Though data is limited at the moment, the Umkondo conglomerates caratage is likely to run into hundreds of millions of carats, with a diamond gem content of between twenty and twenty-five percent, as is indicated from recent diamond production data. The greater part of the Umkondo diamonds are likely to be lodged beneath the deep gravels of the Middle and Lower Save River basin, because small remnant tilted mountain blocks and inselbergs forming the caps of hills are what remains of the host conglomerate on the western margin of the Umkondo Basin. Areas to be examined in this document will be the geology; the mode of formation of the Umkondo basin and its sedimentary system; the regional kimberlite exploration around the basin; and diamond production in the Marange diamond field, in order to come up with indications of the provenance of the diamonds within the Umkondo conglomerates. The kimberlite clusters in and around the Umkondo sedimentary basin have all proved to be barren or only nominally diamondiferous and that the kimberlites are between 200Ma and 500Ma and thus much younger than the greater than 1.1Ga Umkondo diamondiferous conglomerates. Studies so far undertaken have not managed to point to the origin, or provenance, of the Umkondo or Marange diamonds, which were discovered on the western edge of the Umkondo Basin and in the east of the basin below the Chimanimani Mountains along the Haroni River. This paper is an attempt to clear up some of the misconceptions surrounding the Marange diamond deposit and to raise interest in the urgent rquirement to study and understand the Umkondo Basin and the origin of its diamonds. The only meaningful studies on diamond occurrence and diamond exploration of the basin were undertaken from 1996 to 2006 by Kimberlitic Searches Zimbabwe (Pvt) Ltd, the then Zimbabwe kimberlite exploration arm of De Beers, Zimbabwe, in their quest to find kimberlites, which were thought to be related to the Umkondo alluvial diamond deposit. As will be shown in the following chapters, many of the discovered kimberlites range from very low grade to non-diamondiferous, and are much younger than the Umkondo conglomerates, whose diamonds are in turn a great deal older. Thus the basic question concerning the origin or provenance of the Umkondo placer diamonds still remains unresolved. Because of the sheer size of the basin, modern, wide-area-coverage, geophysical exploration methods become appropriate to effectively generate diamond potential targets for further examination. This document will attempt to collate various data available to paint a true picture of the state of exploration within the Umkondo Basin.
- Full Text:
Geospatial technologies and indigenous Knowledge Systems:
- Authors: Aswani, Shankar
- Date: 2016
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/145448 , vital:38439 , ISBN 9781315181523 , https://www.taylorfrancis.com/books/e/9781315181523/chapters/10.1201/9781315181523-18
- Description: During the twentieth and twenty-first centuries, pressure on coastal ecosystems has amplified and resulted in the widespread degradation of adjacent marine and terrestrial habitats globally (Burke et al., 2001). The ecosystem services provided by coastal habitats, including coastal protection and food procurement, have been heavily compromised by anthropogenic disturbance such as overfishing, pollution, sedimentation and alteration of coastal vegetation (Costanza et al., 1997; Agardy et al., 2009). In the context of small islands, this continued degradation in tandem with the ongoing effects of climate change is putting the livelihoods of coastal peoples at risk (e.g. Bell et al., 2009). While international efforts at curtailing these negative trends are ongoing, many researchers are working directly with coastal local/ indigenous communities to seek more effective management of coastal terrestrial and marine resources. Among various approaches, researchers are increasingly incorporating local knowledge systems for designing resource management and conservation plans (e.g. Gadgil et al., 1993).
- Full Text:
- Authors: Aswani, Shankar
- Date: 2016
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/145448 , vital:38439 , ISBN 9781315181523 , https://www.taylorfrancis.com/books/e/9781315181523/chapters/10.1201/9781315181523-18
- Description: During the twentieth and twenty-first centuries, pressure on coastal ecosystems has amplified and resulted in the widespread degradation of adjacent marine and terrestrial habitats globally (Burke et al., 2001). The ecosystem services provided by coastal habitats, including coastal protection and food procurement, have been heavily compromised by anthropogenic disturbance such as overfishing, pollution, sedimentation and alteration of coastal vegetation (Costanza et al., 1997; Agardy et al., 2009). In the context of small islands, this continued degradation in tandem with the ongoing effects of climate change is putting the livelihoods of coastal peoples at risk (e.g. Bell et al., 2009). While international efforts at curtailing these negative trends are ongoing, many researchers are working directly with coastal local/ indigenous communities to seek more effective management of coastal terrestrial and marine resources. Among various approaches, researchers are increasingly incorporating local knowledge systems for designing resource management and conservation plans (e.g. Gadgil et al., 1993).
- Full Text:
Global proliferation of cephalopods
- Doubleday, Zoë A, Prowse, Thomas A A, Arkhipkin, Alexander, Pierce, Graham J, Semmens, Jayson, Steer, Michael, Leporati, Stephen C, Lourenço, Sílvia, Quetglas, Antoni, Sauer, Warwick H H, Gillanders, Bronwyn M
- Authors: Doubleday, Zoë A , Prowse, Thomas A A , Arkhipkin, Alexander , Pierce, Graham J , Semmens, Jayson , Steer, Michael , Leporati, Stephen C , Lourenço, Sílvia , Quetglas, Antoni , Sauer, Warwick H H , Gillanders, Bronwyn M
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/124560 , vital:35628 , https://doi.10.1016/j.cub.2016.04.002
- Description: Human activities have substantially changed the world’s oceans in recent decades, altering marine food webs, habitats and biogeochemical processes [1]. Cephalopods (squid, cuttlefish and octopuses) have a unique set of biological traits, including rapid growth, short lifespans and strong life-history plasticity, allowing them to adapt quickly to changing environmental conditions [2–4]. There has been growing speculation that cephalopod populations are proliferating in response to a changing environment, a perception fuelled by increasing trends in cephalopod fisheries catch [4,5]. To investigate long-term trends in cephalopod abundance, we assembled global time-series of cephalopod catch rates (catch per unit of fishing or sampling effort). We show that cephalopod populations have increased over the last six decades, a result that was remarkably consistent across a highly diverse set of cephalopod taxa. Positive trends were also evident for both fisheries-dependent and fisheries independent time-series, suggesting that trends are not solely due to factors associated with developing fisheries. Our results suggest that large-scale, directional processes, common to a range of coastal and oceanic environments, are responsible. This study presents the first evidence that cephalopod populations have increased globally, indicating that these ecologically and commercially important invertebrates may have benefited from a changing ocean environment.
- Full Text:
- Authors: Doubleday, Zoë A , Prowse, Thomas A A , Arkhipkin, Alexander , Pierce, Graham J , Semmens, Jayson , Steer, Michael , Leporati, Stephen C , Lourenço, Sílvia , Quetglas, Antoni , Sauer, Warwick H H , Gillanders, Bronwyn M
- Date: 2016
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/124560 , vital:35628 , https://doi.10.1016/j.cub.2016.04.002
- Description: Human activities have substantially changed the world’s oceans in recent decades, altering marine food webs, habitats and biogeochemical processes [1]. Cephalopods (squid, cuttlefish and octopuses) have a unique set of biological traits, including rapid growth, short lifespans and strong life-history plasticity, allowing them to adapt quickly to changing environmental conditions [2–4]. There has been growing speculation that cephalopod populations are proliferating in response to a changing environment, a perception fuelled by increasing trends in cephalopod fisheries catch [4,5]. To investigate long-term trends in cephalopod abundance, we assembled global time-series of cephalopod catch rates (catch per unit of fishing or sampling effort). We show that cephalopod populations have increased over the last six decades, a result that was remarkably consistent across a highly diverse set of cephalopod taxa. Positive trends were also evident for both fisheries-dependent and fisheries independent time-series, suggesting that trends are not solely due to factors associated with developing fisheries. Our results suggest that large-scale, directional processes, common to a range of coastal and oceanic environments, are responsible. This study presents the first evidence that cephalopod populations have increased globally, indicating that these ecologically and commercially important invertebrates may have benefited from a changing ocean environment.
- Full Text:
Goeie maniere en etiket
- Authors: Van Staden, Antoinique
- Date: 2016
- Language: Afrikaans , English
- Type: Thesis , Masters , MA
- Identifier: vital:6011 , http://hdl.handle.net/10962/d1021226
- Description: This bilingual collection of short stories combines the fairy tale form and the Bizarro genre to explore the value system ingrained in me at a very early age via my conservative Afrikaans upbringing. To my mind the four characteristics of the fairy tale form as identified by Kate Bernheimer (in her path-breaking essay “Fairy Tale is form, Form is Fairy Tale”) namely flatness, abstraction, intuitive logic and normalized magic, also apply to the Bizarro genre. This intersection is exemplified by some of Bernheimer's own fiction, as well as the fiction of Aimee Bender and Lucy Corin, among others, all of whom have influenced my writing. , Hierdie tweetalige versameling kortverhale kombineer sprokieselemente met die van die Bizarro-genre, om die waardesisteem wat van kleins af deur my konserwatiewe Afrikaanse opvoeding by my ingeprent is, te ondersoek. Die vier eienskappe van die sprokie word deur Kate Bernheimer in haar baanbrekende opstel Fairy tale is form, Form is fairy tale uitgelê as: ’n onbetrokke verteller, abstraksie, instinktiewe logika en genormaliseerde towerkuns. Na my mening is dié vier eienskappe ook op die Bizarro-genre van toepassing. Hierdie oorvleuling is sigbaar in Bernheimer se fiksie en ook onder andere Lucy Corin en Aimee Bender s'n – skrywers wat my werk beïnvloed het. , This thesis is presented in two parts: Afrikaans and English.
- Full Text:
- Authors: Van Staden, Antoinique
- Date: 2016
- Language: Afrikaans , English
- Type: Thesis , Masters , MA
- Identifier: vital:6011 , http://hdl.handle.net/10962/d1021226
- Description: This bilingual collection of short stories combines the fairy tale form and the Bizarro genre to explore the value system ingrained in me at a very early age via my conservative Afrikaans upbringing. To my mind the four characteristics of the fairy tale form as identified by Kate Bernheimer (in her path-breaking essay “Fairy Tale is form, Form is Fairy Tale”) namely flatness, abstraction, intuitive logic and normalized magic, also apply to the Bizarro genre. This intersection is exemplified by some of Bernheimer's own fiction, as well as the fiction of Aimee Bender and Lucy Corin, among others, all of whom have influenced my writing. , Hierdie tweetalige versameling kortverhale kombineer sprokieselemente met die van die Bizarro-genre, om die waardesisteem wat van kleins af deur my konserwatiewe Afrikaanse opvoeding by my ingeprent is, te ondersoek. Die vier eienskappe van die sprokie word deur Kate Bernheimer in haar baanbrekende opstel Fairy tale is form, Form is fairy tale uitgelê as: ’n onbetrokke verteller, abstraksie, instinktiewe logika en genormaliseerde towerkuns. Na my mening is dié vier eienskappe ook op die Bizarro-genre van toepassing. Hierdie oorvleuling is sigbaar in Bernheimer se fiksie en ook onder andere Lucy Corin en Aimee Bender s'n – skrywers wat my werk beïnvloed het. , This thesis is presented in two parts: Afrikaans and English.
- Full Text:
Gold nanoparticle–based colorimetric probes for dopamine detection
- Authors: Ngomane, Nokuthula
- Date: 2016
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4565 , http://hdl.handle.net/10962/d1021261
- Description: Colorimetric probes have become important tools in analysis and biomedical technology. This thesis reports on the development of such probes for the detection of dopamine (DA). Liquid and different solid state probes were developed utilizing un–functionalized gold nanoparticles (UF–AuNPs). The liquid state probe is based on the growth and aggregation of the UF–AuNPs in the presence of DA. Upon addition of the UF–AuNPs to various concentrations of DA, the shape, size and colour change results in spectral shifts from lower to higher wavelengths. The analyte can be easily monitored by the naked eye from 5.0 nM DA with a calculated limit of detection of 2.5 nM (3σ) under optimal pH. Ascorbic acid (AA) has a potential to interfere with DA detection in solution since it is often present in biological fluids, but in this case the interference was limited to solutions where its concentration was beyond 200 times greater than that of DA. Since most of the previously reported colorimetric probes, especially those for DA are solution based, the main focus of the thesis was in the development of a solid state based colorimetric probe in the form of nanofibre mats. To overcome the interference challenges experienced in the solution studies (the interference by high concentrations of AA), the suitability of molecularly imprinted polymers (MIPs) for the selective detection of DA was investigated. The results showed that the MIPs produced did not play a significant role in enhancing the selectivity towards DA. A probe composed of just the UF–AuNPs and Nylon–6 (UF–AuNPs + N6) was also developed. The UF–AuNPs were synthesized following an in situ reduction method. The probe was only selective to DA and insensitive to other catecholamines at physiological pH. Thus, the probe did not require any addition functionalities to achieve selectivity and sensitive to DA. The liquid state probe and the composite UF–AuNPs + N6 nanofibre probe were successfully applied to a whole blood sample and showed good selectivity towards DA. The simple, sensitive and selective probe could be an excellent alternative for on–site and immediate detection of DA without the use of instrumentation. For quantification of DA using the solid state probe, open–source software imageJ was used to assist in the analysis of the nanofibre colours. It was observed that the intensity of the colour increased with the increase in concentration of DA in a linear fashion. The use of imageJ can also be a great alternative where the colour changes are not so clear or for visually impaired people. The solid state probe developed can detect DA qualitatively and quantitatively. The work also forms a good foundation for development of such probes for other analyte.
- Full Text:
- Authors: Ngomane, Nokuthula
- Date: 2016
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: vital:4565 , http://hdl.handle.net/10962/d1021261
- Description: Colorimetric probes have become important tools in analysis and biomedical technology. This thesis reports on the development of such probes for the detection of dopamine (DA). Liquid and different solid state probes were developed utilizing un–functionalized gold nanoparticles (UF–AuNPs). The liquid state probe is based on the growth and aggregation of the UF–AuNPs in the presence of DA. Upon addition of the UF–AuNPs to various concentrations of DA, the shape, size and colour change results in spectral shifts from lower to higher wavelengths. The analyte can be easily monitored by the naked eye from 5.0 nM DA with a calculated limit of detection of 2.5 nM (3σ) under optimal pH. Ascorbic acid (AA) has a potential to interfere with DA detection in solution since it is often present in biological fluids, but in this case the interference was limited to solutions where its concentration was beyond 200 times greater than that of DA. Since most of the previously reported colorimetric probes, especially those for DA are solution based, the main focus of the thesis was in the development of a solid state based colorimetric probe in the form of nanofibre mats. To overcome the interference challenges experienced in the solution studies (the interference by high concentrations of AA), the suitability of molecularly imprinted polymers (MIPs) for the selective detection of DA was investigated. The results showed that the MIPs produced did not play a significant role in enhancing the selectivity towards DA. A probe composed of just the UF–AuNPs and Nylon–6 (UF–AuNPs + N6) was also developed. The UF–AuNPs were synthesized following an in situ reduction method. The probe was only selective to DA and insensitive to other catecholamines at physiological pH. Thus, the probe did not require any addition functionalities to achieve selectivity and sensitive to DA. The liquid state probe and the composite UF–AuNPs + N6 nanofibre probe were successfully applied to a whole blood sample and showed good selectivity towards DA. The simple, sensitive and selective probe could be an excellent alternative for on–site and immediate detection of DA without the use of instrumentation. For quantification of DA using the solid state probe, open–source software imageJ was used to assist in the analysis of the nanofibre colours. It was observed that the intensity of the colour increased with the increase in concentration of DA in a linear fashion. The use of imageJ can also be a great alternative where the colour changes are not so clear or for visually impaired people. The solid state probe developed can detect DA qualitatively and quantitatively. The work also forms a good foundation for development of such probes for other analyte.
- Full Text:
GPU Accelerated protocol analysis for large and long-term traffic traces
- Nottingham, Alastair Timothy
- Authors: Nottingham, Alastair Timothy
- Date: 2016
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/910 , vital:20002
- Description: This thesis describes the design and implementation of GPF+, a complete general packet classification system developed using Nvidia CUDA for Compute Capability 3.5+ GPUs. This system was developed with the aim of accelerating the analysis of arbitrary network protocols within network traffic traces using inexpensive, massively parallel commodity hardware. GPF+ and its supporting components are specifically intended to support the processing of large, long-term network packet traces such as those produced by network telescopes, which are currently difficult and time consuming to analyse. The GPF+ classifier is based on prior research in the field, which produced a prototype classifier called GPF, targeted at Compute Capability 1.3 GPUs. GPF+ greatly extends the GPF model, improving runtime flexibility and scalability, whilst maintaining high execution efficiency. GPF+ incorporates a compact, lightweight registerbased state machine that supports massively-parallel, multi-match filter predicate evaluation, as well as efficient arbitrary field extraction. GPF+ tracks packet composition during execution, and adjusts processing at runtime to avoid redundant memory transactions and unnecessary computation through warp-voting. GPF+ additionally incorporates a 128-bit in-thread cache, accelerated through register shuffling, to accelerate access to packet data in slow GPU global memory. GPF+ uses a high-level DSL to simplify protocol and filter creation, whilst better facilitating protocol reuse. The system is supported by a pipeline of multi-threaded high-performance host components, which communicate asynchronously through 0MQ messaging middleware to buffer, index, and dispatch packet data on the host system. The system was evaluated using high-end Kepler (Nvidia GTX Titan) and entry level Maxwell (Nvidia GTX 750) GPUs. The results of this evaluation showed high system performance, limited only by device side IO (600MBps) in all tests. GPF+ maintained high occupancy and device utilisation in all tests, without significant serialisation, and showed improved scaling to more complex filter sets. Results were used to visualise captures of up to 160 GB in seconds, and to extract and pre-filter captures small enough to be easily analysed in applications such as Wireshark.
- Full Text:
- Authors: Nottingham, Alastair Timothy
- Date: 2016
- Language: English
- Type: Thesis , Doctoral , PhD
- Identifier: http://hdl.handle.net/10962/910 , vital:20002
- Description: This thesis describes the design and implementation of GPF+, a complete general packet classification system developed using Nvidia CUDA for Compute Capability 3.5+ GPUs. This system was developed with the aim of accelerating the analysis of arbitrary network protocols within network traffic traces using inexpensive, massively parallel commodity hardware. GPF+ and its supporting components are specifically intended to support the processing of large, long-term network packet traces such as those produced by network telescopes, which are currently difficult and time consuming to analyse. The GPF+ classifier is based on prior research in the field, which produced a prototype classifier called GPF, targeted at Compute Capability 1.3 GPUs. GPF+ greatly extends the GPF model, improving runtime flexibility and scalability, whilst maintaining high execution efficiency. GPF+ incorporates a compact, lightweight registerbased state machine that supports massively-parallel, multi-match filter predicate evaluation, as well as efficient arbitrary field extraction. GPF+ tracks packet composition during execution, and adjusts processing at runtime to avoid redundant memory transactions and unnecessary computation through warp-voting. GPF+ additionally incorporates a 128-bit in-thread cache, accelerated through register shuffling, to accelerate access to packet data in slow GPU global memory. GPF+ uses a high-level DSL to simplify protocol and filter creation, whilst better facilitating protocol reuse. The system is supported by a pipeline of multi-threaded high-performance host components, which communicate asynchronously through 0MQ messaging middleware to buffer, index, and dispatch packet data on the host system. The system was evaluated using high-end Kepler (Nvidia GTX Titan) and entry level Maxwell (Nvidia GTX 750) GPUs. The results of this evaluation showed high system performance, limited only by device side IO (600MBps) in all tests. GPF+ maintained high occupancy and device utilisation in all tests, without significant serialisation, and showed improved scaling to more complex filter sets. Results were used to visualise captures of up to 160 GB in seconds, and to extract and pre-filter captures small enough to be easily analysed in applications such as Wireshark.
- Full Text:
Grace “The story of a wise cat and a cruel man.”
- Authors: Phepheng, Maruping
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:6005 , http://hdl.handle.net/10962/d1021213
- Description: Morgan, a widower and police detective, is quickly promoted from constable to captain after a series of successful criminal investigations, creating enemies in the process. One of the convicted and incarcerated is Paper, a gang leader with a violent upbringing. After a lucky release from prison, Paper, now Morgan’s fierce foe, plots to hurt him in the worst way possible – by killing his only son. The story is narrated by a cat named Grace, whose presence and companionship comes from another dimension and helps Morgan to keep sane.
- Full Text:
- Authors: Phepheng, Maruping
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:6005 , http://hdl.handle.net/10962/d1021213
- Description: Morgan, a widower and police detective, is quickly promoted from constable to captain after a series of successful criminal investigations, creating enemies in the process. One of the convicted and incarcerated is Paper, a gang leader with a violent upbringing. After a lucky release from prison, Paper, now Morgan’s fierce foe, plots to hurt him in the worst way possible – by killing his only son. The story is narrated by a cat named Grace, whose presence and companionship comes from another dimension and helps Morgan to keep sane.
- Full Text:
Gradient high performance liquid chromatographic method for the simultaneous analysis of efavirenz, emtricitabine and tenofovir
- Authors: Koekemoer, Sonya Mariana
- Date: 2016
- Language: English
- Type: text , Thesis , Masters , MPharm
- Identifier: http://hdl.handle.net/10962/54679 , vital:26599
- Description: In 2014, approximately 6.8 million people in South Africa were HIV-positive, and the majority of those affected are aged 15 or older. A fixed-dose combination (FDC) antiretroviral (ARV) dosage form containing one non-nucleotide reverse transcriptase inhibitor (efavirenz) and two nucleotide reverse transcriptase inhibitors (emtricitabine and tenofovir) was licensed in South Africa in April 2013. New consolidated guidelines for HIV management and prevention of mother to child transmission (PMTCT) were published by the South African Department of Health in December 2014 and the FDC is now the recommended first-line treatment for HIV-positive patients. According to these guidelines all such people aged 15 and older, and weighing more than 40 kg, with a CD4 count of ≤ 500/ μl will be eligible for antiretroviral therapy (ART) using the FDC. In addition every pregnant and breastfeeding woman is eligible for lifelong ART regardless of CD4 count and EFV can be used as first-line treatment for pregnant women regardless of the length of gestation state of the pregnancy at that time. The use of this simplified regime is likely to promote much needed and improved adherence to therapy. An investigation into the development of a stability-indicating reversed-phase high performance liquid chromatography (RP-HPLC) method for the simultaneous quantitation of EFV, FTC and TNF was undertaken. Isocratic HPLC analysis was found to be unsuitable due to the highly polar FTC molecule eluting in the void. Therefore a gradient HPLC method was developed and validated. The method was validated according to the International Conference on Harmonisation, now known as International Council for Harmonization (ICH). Correlation coefficients > 0.999 were obtained for each assessment of linearity and FTC, TNF and EFV are linear in the range 0.4-40 μg/ml, 0.6-60 μg/ml and 1.2-120 μg/ml. The equation of the best-fit least squares regression lines for FTC, TNF and EFV were y = 0.0191x+0.0007, y = 0.0163x+0.0116 and y = 0.01x+0.016, respectively. The method is accurate as the y-intercept was < 2% of the detector response for all ARV, and the method is precise in terms of intra- and inter-assay precision as all % RSD < 2%. The stability-indicating nature of the method was demonstrated under acidic, alkaline and oxidative stress in addition to UV exposure and elevated temperatures, and the individual chromatograms were overlaid using Empower® 3 Software to establish whether there was interference with the peaks of interest. The forced degradation studies demonstrated the selectivity of the method for the ARV compounds. The method was applied to assay and in vitro dissolution studies of commercially available tablets. The amount of each active ingredient released from Atripla® was determined and compared to the amount of each drug released from Aspen Efavirenz® and Truvada® (a combination of FTC and TNF). The percent FTC released from Atripla® and Truvada® was similar based on the acceptance criteria for immediate-release BCS class 1 compounds. Statistical analysis was undertaken to compare the dissolution profiles of FTC, TNF and EFV. The percent of these compounds released in these studies indicate that bioequivalence testing would be required to declare these products interchangeable. The validated RP-HPLC and in vitro dissolution test method are suitable for routine quality control testing of solid oral dosage forms containing EFV, FTC and TNF, and as the dissolution method can discriminate between different formulations of the same molecule, these tools can also be used for analysis during formulation development studies. The method is not suitable for the analysis of the ARV plasma due to lack of sensitivity and an inability to quantitate the compounds at the required concentration levels. The use of HPLC with mass spectroscopy for quantitation would enhance the sensitivity of the method and may eliminate the quantitation of the molecules in the presence of interference that was observed when using UV detection. Fixed dose combination tablets are convenient for patient therapy and it is likely that in the future more molecules will be formulated into such dosage forms. However formulations such as these can pose significant difficulties when developing and using analytical methods for the quantitation of all compounds in the dosage form at the same time, in particular when the compounds have vastly different physico-chemical properties that impact the quality of a separation and therefore the analysis. Therefore when embarking on the development of FDC product cognisance of the difficulties of developing single methods for the analyses is required and approaches to overcome these difficulties should be considered.
- Full Text:
- Authors: Koekemoer, Sonya Mariana
- Date: 2016
- Language: English
- Type: text , Thesis , Masters , MPharm
- Identifier: http://hdl.handle.net/10962/54679 , vital:26599
- Description: In 2014, approximately 6.8 million people in South Africa were HIV-positive, and the majority of those affected are aged 15 or older. A fixed-dose combination (FDC) antiretroviral (ARV) dosage form containing one non-nucleotide reverse transcriptase inhibitor (efavirenz) and two nucleotide reverse transcriptase inhibitors (emtricitabine and tenofovir) was licensed in South Africa in April 2013. New consolidated guidelines for HIV management and prevention of mother to child transmission (PMTCT) were published by the South African Department of Health in December 2014 and the FDC is now the recommended first-line treatment for HIV-positive patients. According to these guidelines all such people aged 15 and older, and weighing more than 40 kg, with a CD4 count of ≤ 500/ μl will be eligible for antiretroviral therapy (ART) using the FDC. In addition every pregnant and breastfeeding woman is eligible for lifelong ART regardless of CD4 count and EFV can be used as first-line treatment for pregnant women regardless of the length of gestation state of the pregnancy at that time. The use of this simplified regime is likely to promote much needed and improved adherence to therapy. An investigation into the development of a stability-indicating reversed-phase high performance liquid chromatography (RP-HPLC) method for the simultaneous quantitation of EFV, FTC and TNF was undertaken. Isocratic HPLC analysis was found to be unsuitable due to the highly polar FTC molecule eluting in the void. Therefore a gradient HPLC method was developed and validated. The method was validated according to the International Conference on Harmonisation, now known as International Council for Harmonization (ICH). Correlation coefficients > 0.999 were obtained for each assessment of linearity and FTC, TNF and EFV are linear in the range 0.4-40 μg/ml, 0.6-60 μg/ml and 1.2-120 μg/ml. The equation of the best-fit least squares regression lines for FTC, TNF and EFV were y = 0.0191x+0.0007, y = 0.0163x+0.0116 and y = 0.01x+0.016, respectively. The method is accurate as the y-intercept was < 2% of the detector response for all ARV, and the method is precise in terms of intra- and inter-assay precision as all % RSD < 2%. The stability-indicating nature of the method was demonstrated under acidic, alkaline and oxidative stress in addition to UV exposure and elevated temperatures, and the individual chromatograms were overlaid using Empower® 3 Software to establish whether there was interference with the peaks of interest. The forced degradation studies demonstrated the selectivity of the method for the ARV compounds. The method was applied to assay and in vitro dissolution studies of commercially available tablets. The amount of each active ingredient released from Atripla® was determined and compared to the amount of each drug released from Aspen Efavirenz® and Truvada® (a combination of FTC and TNF). The percent FTC released from Atripla® and Truvada® was similar based on the acceptance criteria for immediate-release BCS class 1 compounds. Statistical analysis was undertaken to compare the dissolution profiles of FTC, TNF and EFV. The percent of these compounds released in these studies indicate that bioequivalence testing would be required to declare these products interchangeable. The validated RP-HPLC and in vitro dissolution test method are suitable for routine quality control testing of solid oral dosage forms containing EFV, FTC and TNF, and as the dissolution method can discriminate between different formulations of the same molecule, these tools can also be used for analysis during formulation development studies. The method is not suitable for the analysis of the ARV plasma due to lack of sensitivity and an inability to quantitate the compounds at the required concentration levels. The use of HPLC with mass spectroscopy for quantitation would enhance the sensitivity of the method and may eliminate the quantitation of the molecules in the presence of interference that was observed when using UV detection. Fixed dose combination tablets are convenient for patient therapy and it is likely that in the future more molecules will be formulated into such dosage forms. However formulations such as these can pose significant difficulties when developing and using analytical methods for the quantitation of all compounds in the dosage form at the same time, in particular when the compounds have vastly different physico-chemical properties that impact the quality of a separation and therefore the analysis. Therefore when embarking on the development of FDC product cognisance of the difficulties of developing single methods for the analyses is required and approaches to overcome these difficulties should be considered.
- Full Text:
Graphene quantum dots functionalized with 4-amino-2, 2, 6, 6-tetramethylpiperidine-N-oxide as fluorescence “turn-on” nanosensors
- Achadu, Ojodomo J, Britton, Jonathan, Nyokong, Tebello
- Authors: Achadu, Ojodomo J , Britton, Jonathan , Nyokong, Tebello
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/239854 , vital:50773 , xlink:href="https://doi.org/10.1007/s10895-016-1916-y"
- Description: In this study, we report on the fabrication of simple and rapid graphene quantum dots (GQDs)-based fluorescence “turn-ON” nanoprobes for sensitive and selective detection of ascorbic acid (AA). Pristine GQDs and S and N co-doped-GQDs (SN-GQDs) were functionalized with 4-amino-2,2,6,6-tetramethylpiperidine-N-oxide (4-amino-TEMPO, a nitroxide free radical). The nitroxide free radicals efficiently quenched the fluorescence of the GQDs and upon interaction of the nanoconjugates with ascorbic acid, the quenched fluorescence was restored. The linear ranges recorded were 0.5–5.7 μM and 0.1–5.5 μM for GQDs-4-amino-TEMPO and SN-GQDs-4amino-TEMPO nanoprobes, respectively. Limits of detection were found to be 60 nM and 84 nM for SN-GQDS-4-amino-TEMPO and GQDs-4-amino-TEMPO for AA detection, respectively. This novel fluorescence “turn-ON” technique showed to be highly rapid and selective towards AA detection.
- Full Text:
- Authors: Achadu, Ojodomo J , Britton, Jonathan , Nyokong, Tebello
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/239854 , vital:50773 , xlink:href="https://doi.org/10.1007/s10895-016-1916-y"
- Description: In this study, we report on the fabrication of simple and rapid graphene quantum dots (GQDs)-based fluorescence “turn-ON” nanoprobes for sensitive and selective detection of ascorbic acid (AA). Pristine GQDs and S and N co-doped-GQDs (SN-GQDs) were functionalized with 4-amino-2,2,6,6-tetramethylpiperidine-N-oxide (4-amino-TEMPO, a nitroxide free radical). The nitroxide free radicals efficiently quenched the fluorescence of the GQDs and upon interaction of the nanoconjugates with ascorbic acid, the quenched fluorescence was restored. The linear ranges recorded were 0.5–5.7 μM and 0.1–5.5 μM for GQDs-4-amino-TEMPO and SN-GQDs-4amino-TEMPO nanoprobes, respectively. Limits of detection were found to be 60 nM and 84 nM for SN-GQDS-4-amino-TEMPO and GQDs-4-amino-TEMPO for AA detection, respectively. This novel fluorescence “turn-ON” technique showed to be highly rapid and selective towards AA detection.
- Full Text:
HealthMessenger: an e-Health service to support ICT deployments in poor areas
- Gremu, Chikumbutso, Terzoli, Alfredo, Tsietsi, Mosiuoa
- Authors: Gremu, Chikumbutso , Terzoli, Alfredo , Tsietsi, Mosiuoa
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/430609 , vital:72703 , 10.1109/ISTAFRICA.2016.7530632
- Description: Development and implementation of appropriate e-services as well as revenue generation are key to deploying and sustaining ICT installa-tions in poor areas in developing countries. The area of e-Health is a promising area for e-Services that are important to populations in poor areas and health service organisations that are already spending mon-ey on different health initiatives in these areas. This paper discusses an e-Health service that facilitates dissemination of health information to people living in poor areas and that is subsequently used to generate revenue to support deployment and development of ICT in the areas. The tool was developed within the context of the Siyakhula Living Lab (SLL), a multi-stakeholder operation that promotes ICT for Development in the Eastern Cape Province of South Africa. Creation of health con-tent for the e-health service and its subsequent consumption happen in two different environments from a technological and social point of view. To enable this to happen, the e-service comprises two component applications that run in a peer-to-peer fashion. The component that is used to disseminate content for end-user consumption is called the HealthMessenger. The HealthMessenger is hosted on an environment called TeleWeaver, an application integration platform developed within the SLL to host software applications targeting people living in poor ar-eas. The platform is customised to support services with a revenue generation component.
- Full Text:
- Authors: Gremu, Chikumbutso , Terzoli, Alfredo , Tsietsi, Mosiuoa
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/430609 , vital:72703 , 10.1109/ISTAFRICA.2016.7530632
- Description: Development and implementation of appropriate e-services as well as revenue generation are key to deploying and sustaining ICT installa-tions in poor areas in developing countries. The area of e-Health is a promising area for e-Services that are important to populations in poor areas and health service organisations that are already spending mon-ey on different health initiatives in these areas. This paper discusses an e-Health service that facilitates dissemination of health information to people living in poor areas and that is subsequently used to generate revenue to support deployment and development of ICT in the areas. The tool was developed within the context of the Siyakhula Living Lab (SLL), a multi-stakeholder operation that promotes ICT for Development in the Eastern Cape Province of South Africa. Creation of health con-tent for the e-health service and its subsequent consumption happen in two different environments from a technological and social point of view. To enable this to happen, the e-service comprises two component applications that run in a peer-to-peer fashion. The component that is used to disseminate content for end-user consumption is called the HealthMessenger. The HealthMessenger is hosted on an environment called TeleWeaver, an application integration platform developed within the SLL to host software applications targeting people living in poor ar-eas. The platform is customised to support services with a revenue generation component.
- Full Text:
Hearing things
- Authors: Claassen, José
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:6019 , http://hdl.handle.net/10962/d1021243
- Description: William Carlos Williams wrote: "It isn't what the poet says that counts as a work of art, it's what he makes, with such intensity of purpose that it lives with an intrinsic movement of its own to verify its authenticity." I would like to think that my poems only borrow life from my pen, taking on an identity and music of their own with the help of some ‘making’ on my part. My poems embrace a continuum of human experience from the intrapersonal to the societal. Using imagistic and cinematic forms, they preserve the vitality of their sources, from the music of cityscapes, to the texture of emotions, to the narratives of particular characters.
- Full Text:
- Authors: Claassen, José
- Date: 2016
- Language: English
- Type: Thesis , Masters , MA
- Identifier: vital:6019 , http://hdl.handle.net/10962/d1021243
- Description: William Carlos Williams wrote: "It isn't what the poet says that counts as a work of art, it's what he makes, with such intensity of purpose that it lives with an intrinsic movement of its own to verify its authenticity." I would like to think that my poems only borrow life from my pen, taking on an identity and music of their own with the help of some ‘making’ on my part. My poems embrace a continuum of human experience from the intrapersonal to the societal. Using imagistic and cinematic forms, they preserve the vitality of their sources, from the music of cityscapes, to the texture of emotions, to the narratives of particular characters.
- Full Text:
Heat accumulation and development rate of massed maggots of the sheep blowfly, Lucilia cuprina (Diptera: Calliphoridae)
- Kotzé, Zanthé, Villet, Martin H, Weldon, Christopher W
- Authors: Kotzé, Zanthé , Villet, Martin H , Weldon, Christopher W
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/442168 , vital:73964 , https://doi.org/10.1016/j.jinsphys.2016.09.009
- Description: Blowfly larvae aggregate on exposed carcasses and corpses and pass through three instars before wandering from the carcass and pupating. The developmental landmarks in this process can be used by forensic entomologists to estimate the time since the insects colonised the carcass, which sets a minimum post mortem interval. Large aggregations of feeding larvae generate a microclimate with temperatures up to 15 °C above ambient conditions, which may accelerate larval development and affect forensic estimates of post-mortem intervals. This study investigated the effects of heat accumulated by maggot masses of Lucilia cuprina at aggregations of 20, 50 and 100 larvae, each at incubation temperatures of 18 °C, 24 °C and 30 °C, using body length and life stage as developmental indicators. Aggregation temperatures reached up to 18.7 °C above ambient temperature, with significant effects of both size and temperature of the aggregation on the development time of its larvae. Survivorship was highest for all life stages at 24 °C, which is near the developmental optimum of L. cuprina. The results of this study provide a broadly applicable method of quantifying heat accumulation by aggregations of a wide range of species of forensic importance, and the results obtained from such studies will demonstrate that ambient temperature cannot be considered the only source of heat that blowfly larvae experience when they develop on a carcass. Neglect of temperatures within larval aggregations will result in an overestimation of post-mortem intervals and thus have far-reaching medicolegal consequences.
- Full Text:
- Authors: Kotzé, Zanthé , Villet, Martin H , Weldon, Christopher W
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/442168 , vital:73964 , https://doi.org/10.1016/j.jinsphys.2016.09.009
- Description: Blowfly larvae aggregate on exposed carcasses and corpses and pass through three instars before wandering from the carcass and pupating. The developmental landmarks in this process can be used by forensic entomologists to estimate the time since the insects colonised the carcass, which sets a minimum post mortem interval. Large aggregations of feeding larvae generate a microclimate with temperatures up to 15 °C above ambient conditions, which may accelerate larval development and affect forensic estimates of post-mortem intervals. This study investigated the effects of heat accumulated by maggot masses of Lucilia cuprina at aggregations of 20, 50 and 100 larvae, each at incubation temperatures of 18 °C, 24 °C and 30 °C, using body length and life stage as developmental indicators. Aggregation temperatures reached up to 18.7 °C above ambient temperature, with significant effects of both size and temperature of the aggregation on the development time of its larvae. Survivorship was highest for all life stages at 24 °C, which is near the developmental optimum of L. cuprina. The results of this study provide a broadly applicable method of quantifying heat accumulation by aggregations of a wide range of species of forensic importance, and the results obtained from such studies will demonstrate that ambient temperature cannot be considered the only source of heat that blowfly larvae experience when they develop on a carcass. Neglect of temperatures within larval aggregations will result in an overestimation of post-mortem intervals and thus have far-reaching medicolegal consequences.
- Full Text:
Heat shock protein inhibitors: success stories
- McAlpine, Shelli R, Edkins, Adrienne L
- Authors: McAlpine, Shelli R , Edkins, Adrienne L
- Date: 2016
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/66359 , vital:28940 , https://doi.org/10.1007/978-3-319-32607-8
- Description: publisher version , Introduction: Medicinal chemistry is both science and art. The science of medicinal chemistry offers mankind one of its best hopes for improving the quality of life. The art of medicinal chemistry continues to challenge its practitioners with the need for both intuition and experience to discover new drugs. Hence sharing the experience of drug research is uniquely beneficial to the field of medicinal chemistry. Drug research requires interdisciplinary team-work at the interface between chemistry, biology and medicine. Therefore, the topic-related series Topics in Medicinal Chemistry covers all relevant aspects of drug research, e.g. pathobiochemistry of diseases, identification and validation of (emerging) drug targets, structural biology, drugability of targets, drug design approaches, chemogenomics, synthetic chemistry including combinatorial methods, bioorganic chemistry, natural compounds, high-throughput screening, pharmacological in vitro and in vivo investigations, drug-receptor interactions on the molecular level, structure-activity relationships, drug absorption, distribution, metabolism, elimination, toxicology and pharmacogenomics. In general, special volumes are edited by well known guest editors. , This work is based on the research supported by the South African Research Chairs Initiative of the Department of Science and Technology and National Research Foundation of South Africa (Grant No 98566), the Cancer Association of South Africa (CANSA), Medical Research Council South Africa (MRC-SA) and Rhodes University. The views expressed are those of the authors and should not be attributed to the DST, NRF, CANSA, MRC-SA or Rhodes University. We apologize if we have inadvertently missed any important contributions to the field.
- Full Text: false
- Authors: McAlpine, Shelli R , Edkins, Adrienne L
- Date: 2016
- Language: English
- Type: text , book
- Identifier: http://hdl.handle.net/10962/66359 , vital:28940 , https://doi.org/10.1007/978-3-319-32607-8
- Description: publisher version , Introduction: Medicinal chemistry is both science and art. The science of medicinal chemistry offers mankind one of its best hopes for improving the quality of life. The art of medicinal chemistry continues to challenge its practitioners with the need for both intuition and experience to discover new drugs. Hence sharing the experience of drug research is uniquely beneficial to the field of medicinal chemistry. Drug research requires interdisciplinary team-work at the interface between chemistry, biology and medicine. Therefore, the topic-related series Topics in Medicinal Chemistry covers all relevant aspects of drug research, e.g. pathobiochemistry of diseases, identification and validation of (emerging) drug targets, structural biology, drugability of targets, drug design approaches, chemogenomics, synthetic chemistry including combinatorial methods, bioorganic chemistry, natural compounds, high-throughput screening, pharmacological in vitro and in vivo investigations, drug-receptor interactions on the molecular level, structure-activity relationships, drug absorption, distribution, metabolism, elimination, toxicology and pharmacogenomics. In general, special volumes are edited by well known guest editors. , This work is based on the research supported by the South African Research Chairs Initiative of the Department of Science and Technology and National Research Foundation of South Africa (Grant No 98566), the Cancer Association of South Africa (CANSA), Medical Research Council South Africa (MRC-SA) and Rhodes University. The views expressed are those of the authors and should not be attributed to the DST, NRF, CANSA, MRC-SA or Rhodes University. We apologize if we have inadvertently missed any important contributions to the field.
- Full Text: false
Herman Charles Bosman: A man of profound contradictions
- Authors: Leff, Carol W
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/458524 , vital:75751 , https://hdl.handle.net/10520/EJC190700
- Description: Herman Charles Bosman is best known for his humorous short stories with signature twist endings. He is less well known for his essays and journalistic writing, which illustrate another side of this enigmatic man. This essay focuses on the paradox that Bosman was (and continues to be) and aims to respond to the following questions: How are we to understand the identity of Herman Charles Bosman? How did he contribute towards an understanding of language and culture in South Africa during his lifetime? Was Bosman consistent in expressing his views through his literary works, or is there evidence of incongruity? These complexities are explored by, first, paying close attention to various biographies on Bosman. His hybrid identity is then illustrated by providing examples of the many pseudonyms he employed over a period of time. This is followed by discussion of several of Bosman's non-fiction pieces, highlighting how contradictions are apparent in his beliefs and philosophy. The essay concludes that Bosman vacillated in both his writing and his beliefs, and was indeed a man of contradictions.
- Full Text:
- Authors: Leff, Carol W
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/458524 , vital:75751 , https://hdl.handle.net/10520/EJC190700
- Description: Herman Charles Bosman is best known for his humorous short stories with signature twist endings. He is less well known for his essays and journalistic writing, which illustrate another side of this enigmatic man. This essay focuses on the paradox that Bosman was (and continues to be) and aims to respond to the following questions: How are we to understand the identity of Herman Charles Bosman? How did he contribute towards an understanding of language and culture in South Africa during his lifetime? Was Bosman consistent in expressing his views through his literary works, or is there evidence of incongruity? These complexities are explored by, first, paying close attention to various biographies on Bosman. His hybrid identity is then illustrated by providing examples of the many pseudonyms he employed over a period of time. This is followed by discussion of several of Bosman's non-fiction pieces, highlighting how contradictions are apparent in his beliefs and philosophy. The essay concludes that Bosman vacillated in both his writing and his beliefs, and was indeed a man of contradictions.
- Full Text:
Heterogeneity in virulence relationships between Cryptophlebia leucotreta granulovirus isolates and geographically distinct host populations: Lessons from codling moth resistance to CpGV-M
- Opoku-Debrah, John K, Hill, Martin P, Knox, Caroline M, Moore, Sean D
- Authors: Opoku-Debrah, John K , Hill, Martin P , Knox, Caroline M , Moore, Sean D
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/417998 , vital:71500 , xlink:href="https://doi.org/10.1007/s10526-016-9728-1"
- Description: In South Africa, the baculovirus Cryptophlebia leucotreta granulovirus (CrleGV) is used to control the citrus pest Thaumatotibia leucotreta (Meyrick) (Lepidoptera: Tortricidae). However, the risk associated with continuous application of a single active ingredient is resistance. In order to manage resistance should it occur in field populations of T. leucotreta in South Africa, five new CrleGV genotypes from geographically distinct insect populations which were shown to exhibit some degree of phenotypic variation were recovered and genetically characterized. In droplet bioassays using seven CrleGV isolates against five T. leucotreta populations, some isolates were found to show higher virulence to some host populations than others. There were marked differences in the LD50 values of isolates and the number of occlusion bodies required per larva ranged between 0.79 and 3.12. The significance of these findings with respect to the application of CrleGV biopesticides and the management of resistance is discussed.
- Full Text:
- Authors: Opoku-Debrah, John K , Hill, Martin P , Knox, Caroline M , Moore, Sean D
- Date: 2016
- Subjects: To be catalogued
- Language: English
- Type: text , article
- Identifier: http://hdl.handle.net/10962/417998 , vital:71500 , xlink:href="https://doi.org/10.1007/s10526-016-9728-1"
- Description: In South Africa, the baculovirus Cryptophlebia leucotreta granulovirus (CrleGV) is used to control the citrus pest Thaumatotibia leucotreta (Meyrick) (Lepidoptera: Tortricidae). However, the risk associated with continuous application of a single active ingredient is resistance. In order to manage resistance should it occur in field populations of T. leucotreta in South Africa, five new CrleGV genotypes from geographically distinct insect populations which were shown to exhibit some degree of phenotypic variation were recovered and genetically characterized. In droplet bioassays using seven CrleGV isolates against five T. leucotreta populations, some isolates were found to show higher virulence to some host populations than others. There were marked differences in the LD50 values of isolates and the number of occlusion bodies required per larva ranged between 0.79 and 3.12. The significance of these findings with respect to the application of CrleGV biopesticides and the management of resistance is discussed.
- Full Text:
Historical perspectives and future directions for access to land, water and related ecosystem services in the Lower Sundays River Valley, South Africa: implications for human well being
- Authors: Chadzingwa, Karabo
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/442 , vital:19959
- Description: The legacy of South Africa’s history has facilitated unequal access to land and water resources. In the Lower Sundays River Valley (LSRV), a predominantly commercial farming area, differential access to land and water has impacted aspects of ecosystem service access and human well‐being for disenfranchised families over time. Despite the social, political and economic reform efforts in the past two decades, severe inertia towards efforts attempting to increase equitable access to land and water has been experienced. As a result, communities have mobilized and claimed their land from the government through the land restitution process. Based on a mixed‐methods approach, this research explores the ways in which access to land and water over time has influenced current levels of human well‐being among disenfranchised families. Provisioning and cultural ecosystem services were identified as key areas of loss as a result of forced evictions from land. Freedom of choice was a central and cross‐cutting theme regarding the ability to change levels of human well‐being. Although the loss of ecosystem services associated with land and water had an impact on households, the ecosystem services which are regarded as fundamental to human well‐being do not seem to have been lost. The study recommends the wide use of freedom of choice as an indicator for well‐being in the LSRV, as well as a consideration of subjective, objective and psychological measures of well‐being with regards to natural resources and ecosystem services access. Key agents in fostering desirable pathways toward equity and sustainability in the LSRV are identified with stakeholders as private businesses; inclusive governance; empowered and skilled individuals, as well as NGOs.
- Full Text:
- Authors: Chadzingwa, Karabo
- Date: 2016
- Language: English
- Type: Thesis , Masters , MSc
- Identifier: http://hdl.handle.net/10962/442 , vital:19959
- Description: The legacy of South Africa’s history has facilitated unequal access to land and water resources. In the Lower Sundays River Valley (LSRV), a predominantly commercial farming area, differential access to land and water has impacted aspects of ecosystem service access and human well‐being for disenfranchised families over time. Despite the social, political and economic reform efforts in the past two decades, severe inertia towards efforts attempting to increase equitable access to land and water has been experienced. As a result, communities have mobilized and claimed their land from the government through the land restitution process. Based on a mixed‐methods approach, this research explores the ways in which access to land and water over time has influenced current levels of human well‐being among disenfranchised families. Provisioning and cultural ecosystem services were identified as key areas of loss as a result of forced evictions from land. Freedom of choice was a central and cross‐cutting theme regarding the ability to change levels of human well‐being. Although the loss of ecosystem services associated with land and water had an impact on households, the ecosystem services which are regarded as fundamental to human well‐being do not seem to have been lost. The study recommends the wide use of freedom of choice as an indicator for well‐being in the LSRV, as well as a consideration of subjective, objective and psychological measures of well‐being with regards to natural resources and ecosystem services access. Key agents in fostering desirable pathways toward equity and sustainability in the LSRV are identified with stakeholders as private businesses; inclusive governance; empowered and skilled individuals, as well as NGOs.
- Full Text:
Hoofletter K Kleurling - 'n besonderse Suid-Afrikaanse
- Authors: Thomas, Cornelius
- Date: 2016
- Language: Afrikaans
- Type: book chapter , text
- Identifier: http://hdl.handle.net/10962/59771 , vital:27647 , http://naledi.online/product/ons-kom-van-ver/
- Description: Hoofletter K Kleurling.
- Full Text: false
- Authors: Thomas, Cornelius
- Date: 2016
- Language: Afrikaans
- Type: book chapter , text
- Identifier: http://hdl.handle.net/10962/59771 , vital:27647 , http://naledi.online/product/ons-kom-van-ver/
- Description: Hoofletter K Kleurling.
- Full Text: false